Rroxscaffold_164G00435970

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000164
OG0017098
Physical Location & Seq
Forward (+)
16478 .. 17740
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_164G00435970.1

Sequence Viewer

Length: 1263 bp
ATGGCCCCCGACCAAGACGACGCCGTTCTCCCCGAGGGCGACCTCTCCTCCTCCTCCCAAGACGACGAGGAAGAAGACGACGTCGTTGACGAGGACGATGATGACGACGTCGACGACGACGATGACGTCATCAACTCCGCCTCTCCTTCCTCCACCTCCAATTCCCTCCCCGTCGCCATAGCCACCGCTCCTCCCACCGTCACCGTCGCGCTCCCGGCCCCGCTCGCCACAACCGCGCCGGCGGCGGCCACGACGGCGGTGGTTTCCGAGGGTTTGGATCCGAAGCGGCCGCGGACGATTCAGTACGACCCGGTCTTGCTGCCGCTGGCGGCGCCGGAGGAGAAGAAGCCGGCGGCGGCGCTGGACGACTCGAGGAGGCTGTTCCAGCGGCTGTGGACGGACGAGGACGAGATCGAGCTACTGCAAGGGTTTCTGGACTACACGACGCAGAGAGGGAACAAAGGCGGGTCGCACCAAAACGACACCGCTTTGTTCTACGATCAGATCAAGTCGAAGCTCCAGCTGGAATTCAACAAGAACCAGCTGGTGGAGAAGCTCCGGCGCCTGAAGAAGAAGTACCGGAATGTTCTCAACAAAATCGCCAGCGGCAAAGAGGTCAGCTTCAAGAGCCCTCACGATCAGGCCACGTTTGAGATCTCAAGGAAGATATGGAGCAACATCGGCCGTATAGGGGTTGGTGGCGCCGACGACAACGACGACGAAGACCCTAACCCTAACCCTAACAATCCCAATTTCAACCATTTCGAGATCAAGACGGAAGAGGCCGCTGGATTTCAGAAGTCCACGCCAAAATCAAGGAAGCGCCAGCGGTCGCCGGCGCAGCCGAAGACGCCGAGTTTCGTTGAGCCGGCGGTGCCGGTGATCAAGGAGAATCATCACAATGTGAACAATGGCGGTGGCGTTGGTGGTGGTGGGAGTAATTGTGGAAATTGTAATGTTCAGGGGATGATTGAGGAGACCGTGAAGAGTTGTTTGTCTCCTGTGTTGAGGGAGTTGATGAGCAGTTCAATGGGGGGAGGAGGGCCGTCTAGAGGGTTTGGAGGGCTGTCGTTGAATCCAATTCCGCTGAGCTTTGGCGGCCCTTCGATGAGTTTGAATTTTGGAGGTGGGGAAGCGGCGGATGAGCAGTGGAGGAAGCAGCAGATTTTGGAGTTGGAGGTGTATTCGAAGCGATTGGAGTTGGTGCAGAATCAGATAAAAGTGGCATTGGATGAGTTGCGGTCCACAGGAGGAGGAGGCTGA

Protein Analysis

420

Amino Acids

45.33

Weight (kDa)

4.71

Isoelectric Point (pI)

60.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 128 - 225 2.9e-32 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20613
rosa_chinensis RchiOBHm_Chr3g0452191
rosa_laevigata RLG00000013009 RLG00000025619
rosa_multiflora Rmu_sc0021298.1_g000001
rosa_roxburghii Rroxscaffold_164G00435970 Rroxscaffold_6G00429330
rosa_rugosa Rorug02G0639100
rosa_samantha Rh3AG040900 Rh3BG042500 Rh3CG041000 Rh3DG041900
rosa_wichuraiana Rw3G003130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AatII GACGTC 3 cut(s) 84, 111, 129
AccB1I GGYRCC 4 cut(s) 331, 561, 701, 874
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 2 cut(s) 188, 223
AccI GTMKAC 1 cut(s) 111
AccII CGCG 3 cut(s) 209, 236, 292
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 3 cut(s) 246, 287, 682
AcsI RAATTY 2 cut(s) 527, 1117
AcuI CTGAAG 1 cut(s) 587
AcyI GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
AdeI CACNNNGTG 1 cut(s) 904
AfaI GTAC 2 cut(s) 305, 578
AfiI CCNNNNNNNGG 3 cut(s) 547, 691, 1052
AgsI TTSAA 6 cut(s) 532, 625, 757, 1029, 1075, 1117
AluBI AGCT 7 cut(s) 418, 517, 523, 544, 556, 621, 1092
AluI AGCT 7 cut(s) 418, 517, 523, 544, 556, 621, 1092
Alw26I GTCTC 2 cut(s) 971, 1002
AlwI GGATC 2 cut(s) 272, 285
AlwNI CAGNNNCTG 1 cut(s) 391
Ama87I CYCGRG 2 cut(s) 32, 370
AoxI GGCC 9 cut(s) 3, 216, 246, 287, 642, 682, 783, 1043, 1099
ApeKI GCWGC 3 cut(s) 319, 841, 1159
ApoI RAATTY 2 cut(s) 527, 1117
AspLEI GCGC 8 cut(s) 211, 238, 334, 361, 564, 704, 825, 841
AspS9I GGNCC 5 cut(s) 4, 217, 1043, 1100, 1242
AsuC2I CCSGG 2 cut(s) 215, 311
AsuHPI GGTGA 2 cut(s) 193, 892
AsuII TTCGAA 1 cut(s) 1187
AvaI CYCGRG 2 cut(s) 32, 370
AvaII GGWCC 1 cut(s) 1242
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 4 cut(s) 331, 561, 701, 874
BanII GRGCYC 1 cut(s) 632
BarI GAAGNNNNNNTAC 2 cut(s) 560, 592
BbsI GAAGAC 3 cut(s) 81, 729, 854
BbvI GCAGC 3 cut(s) 306, 853, 1171
BceAI ACGGC 4 cut(s) 8, 270, 669, 1030
BclI TGATCA 1 cut(s) 882
BcnI CCSGG 2 cut(s) 215, 311
BcoDI GTCTC 2 cut(s) 971, 1002
BfaI CTAG 1 cut(s) 1050
BfoI RGCGCY 5 cut(s) 335, 362, 565, 705, 826
BglI GCCNNNNNGGC 1 cut(s) 254
BglII AGATCT 1 cut(s) 654
BlpI GCTNAGC 1 cut(s) 1088
Bme1390I CCNGG 2 cut(s) 215, 311
Bme18I GGWCC 1 cut(s) 1242
BmeT110I CYCGRG 2 cut(s) 32, 370
BmgT120I GGNCC 5 cut(s) 4, 217, 1043, 1100, 1242
BmiI GGNNCC 7 cut(s) 6, 219, 279, 333, 563, 703, 876
BmrFI CCNGG 2 cut(s) 215, 311
BpiI GAAGAC 3 cut(s) 81, 729, 854
BpmI CTGGAG 1 cut(s) 503
Bpu1102I GCTNAGC 1 cut(s) 1088
Bpu14I TTCGAA 1 cut(s) 1187
BpuEI CTTGAG 1 cut(s) 643
BpuMI CCSGG 2 cut(s) 215, 311
BsaHI GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
BsaI GGTCTC 1 cut(s) 971
BsaJI CCNNGG 3 cut(s) 33, 267, 290
BsaWI WCCGGW 1 cut(s) 579
BsaXI ACNNNNNCTCC 6 cut(s) 32, 62, 175, 205, 928, 958
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 691, 1052
Bse118I RCCGGY 5 cut(s) 238, 349, 835, 868, 877
BseDI CCNNGG 3 cut(s) 33, 267, 290
BseGI GGATG 3 cut(s) 972, 1147, 1237
BseLI CCNNNNNNNGG 3 cut(s) 547, 691, 1052
BseMII CTCAG 1 cut(s) 1079
BseRI GAGGAG 8 cut(s) 37, 40, 43, 180, 353, 388, 989, 1053
BseX3I CGGCCG 2 cut(s) 287, 682
BseXI GCAGC 3 cut(s) 306, 853, 1171
BsgI GTGCAG 1 cut(s) 1226
Bsh1236I CGCG 3 cut(s) 209, 236, 292
Bsh1285I CGRYCG 3 cut(s) 290, 685, 833
BshFI GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 1045, 1101
BshNI GGYRCC 4 cut(s) 331, 561, 701, 874
BsiEI CGRYCG 3 cut(s) 290, 685, 833
BsiHKCI CYCGRG 2 cut(s) 32, 370
BslI CCNNNNNNNGG 3 cut(s) 547, 691, 1052
BsmAI GTCTC 2 cut(s) 971, 1002
BsnI GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 1045, 1101
Bso31I GGTCTC 1 cut(s) 971
BsoBI CYCGRG 2 cut(s) 32, 370
Bsp119I TTCGAA 1 cut(s) 1187
Bsp1286I GDGCHC 1 cut(s) 632
Bsp143I GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
Bsp1720I GCTNAGC 1 cut(s) 1088
BspANI GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 1045, 1101
BspCNI CTCAG 1 cut(s) 1080
BspFNI CGCG 3 cut(s) 209, 236, 292
BspLI GGNNCC 7 cut(s) 6, 219, 279, 333, 563, 703, 876
BspPI GGATC 2 cut(s) 272, 285
BspT104I TTCGAA 1 cut(s) 1187
BspT107I GGYRCC 4 cut(s) 331, 561, 701, 874
BspTNI GGTCTC 1 cut(s) 971
BsrBI CCGCTC 2 cut(s) 188, 223
BsrFI RCCGGY 5 cut(s) 238, 349, 835, 868, 877
BssAI RCCGGY 5 cut(s) 238, 349, 835, 868, 877
BssECI CCNNGG 3 cut(s) 33, 267, 290
BssMI GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
BssNI GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
Bst4CI ACNGT 3 cut(s) 199, 205, 982
Bst6I CTCTTC 2 cut(s) 774, 980
BstACI GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
BstBI TTCGAA 1 cut(s) 1187
BstC8I GCNNGC 8 cut(s) 225, 240, 327, 351, 604, 827, 837, 870
BstDEI CTNAG 1 cut(s) 1088
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 3 cut(s) 972, 1147, 1237
BstFNI CGCG 3 cut(s) 209, 236, 292
BstH2I RGCGCY 5 cut(s) 335, 362, 565, 705, 826
BstHHI GCGC 8 cut(s) 211, 238, 334, 361, 564, 704, 825, 841
BstKTI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
BstMAI GTCTC 2 cut(s) 971, 1002
BstMBI GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
BstMCI CGRYCG 3 cut(s) 290, 685, 833
BstSCI CCNGG 2 cut(s) 213, 309
BstUI CGCG 3 cut(s) 209, 236, 292
BstV1I GCAGC 3 cut(s) 306, 853, 1171
BstV2I GAAGAC 3 cut(s) 81, 729, 854
BstX2I RGATCY 2 cut(s) 277, 654
BstYI RGATCY 2 cut(s) 277, 654
BstZI CGGCCG 2 cut(s) 287, 682
BsuRI GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 1045, 1101
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 3 cut(s) 972, 1147, 1237
BtsI GCAGTG 1 cut(s) 1154
BtsIMutI CAGTG 1 cut(s) 1154
Cac8I GCNNGC 8 cut(s) 225, 240, 327, 351, 604, 827, 837, 870
CaiI CAGNNNCTG 1 cut(s) 391
CciNI GCGGCCGC 1 cut(s) 287
CfoI GCGC 8 cut(s) 211, 238, 334, 361, 564, 704, 825, 841
Cfr10I RCCGGY 5 cut(s) 238, 349, 835, 868, 877
Cfr13I GGNCC 5 cut(s) 4, 217, 1043, 1100, 1242
Cfr42I CCGCGG 1 cut(s) 293
CseI GACGC 3 cut(s) 29, 454, 859
Csp6I GTAC 2 cut(s) 304, 577
CspCI CAANNNNNGTGG 2 cut(s) 898, 933
CviQI GTAC 2 cut(s) 304, 577
DdeI CTNAG 1 cut(s) 1088
DinI GGCGCC 3 cut(s) 333, 563, 703
DpnI GATC 8 cut(s) 279, 413, 501, 506, 639, 656, 770, 884
DpnII GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
DraIII CACNNNGTG 1 cut(s) 904
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 3 cut(s) 246, 287, 682
EagI CGGCCG 2 cut(s) 287, 682
Eam1104I CTCTTC 2 cut(s) 774, 980
EarI CTCTTC 2 cut(s) 774, 980
EciI GGCGGA 2 cut(s) 127, 1154
EclXI CGGCCG 2 cut(s) 287, 682
Eco24I GRGCYC 1 cut(s) 632
Eco31I GGTCTC 1 cut(s) 971
Eco47I GGWCC 1 cut(s) 1242
Eco52I CGGCCG 2 cut(s) 287, 682
Eco57I CTGAAG 1 cut(s) 587
Eco88I CYCGRG 2 cut(s) 32, 370
EcoRI GAATTC 1 cut(s) 527
EcoT38I GRGCYC 1 cut(s) 632
EgeI GGCGCC 3 cut(s) 333, 563, 703
EheI GGCGCC 3 cut(s) 333, 563, 703
FaiI YATR 3 cut(s) 179, 670, 689
FauI CCCGC 2 cut(s) 228, 458
FbaI TGATCA 1 cut(s) 882
FblI GTMKAC 1 cut(s) 111
FokI GGATG 3 cut(s) 979, 1154, 1244
FriOI GRGCYC 1 cut(s) 632
FspBI CTAG 1 cut(s) 1050
GlaI GCGC 8 cut(s) 210, 237, 333, 360, 563, 703, 824, 840
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 5 cut(s) 335, 362, 565, 705, 826
HaeIII GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 1045, 1101
HgaI GACGC 3 cut(s) 29, 454, 859
HhaI GCGC 8 cut(s) 211, 238, 334, 361, 564, 704, 825, 841
Hin1I GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
Hin6I GCGC 8 cut(s) 209, 236, 332, 359, 562, 702, 823, 839
HinP1I GCGC 8 cut(s) 209, 236, 332, 359, 562, 702, 823, 839
HincII GTYRAC 2 cut(s) 88, 112
HindII GTYRAC 2 cut(s) 88, 112
HinfI GANTC 5 cut(s) 298, 368, 892, 1075, 1210
HphI GGTGA 2 cut(s) 193, 892
Hpy166II GTNNAC 6 cut(s) 88, 112, 396, 804, 907, 1245
Hpy188I TCNGA 5 cut(s) 268, 282, 504, 798, 1215
Hpy188III TCNNGA 6 cut(s) 434, 625, 635, 766, 772, 1050
Hpy8I GTNNAC 6 cut(s) 88, 112, 396, 804, 907, 1245
HpyAV CCTTC 2 cut(s) 156, 1113
HpyCH4III ACNGT 3 cut(s) 199, 205, 982
HpyCH4IV ACGT 4 cut(s) 81, 108, 126, 647
HpyCH4V TGCA 2 cut(s) 424, 1207
HpyF3I CTNAG 1 cut(s) 1088
HpySE526I ACGT 4 cut(s) 81, 108, 126, 647
Hsp92I GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
HspAI GCGC 8 cut(s) 209, 236, 332, 359, 562, 702, 823, 839
KasI GGCGCC 3 cut(s) 331, 561, 701
KroI GCCGGC 4 cut(s) 238, 349, 835, 868
KroNI GCCGGC 4 cut(s) 240, 351, 837, 870
Ksp22I TGATCA 1 cut(s) 882
KspI CCGCGG 1 cut(s) 293
Kzo9I GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
LmnI GCTCC 5 cut(s) 193, 216, 522, 561, 672
Lsp1109I GCAGC 3 cut(s) 306, 853, 1171
MaeI CTAG 1 cut(s) 1050
MaeII ACGT 4 cut(s) 81, 108, 126, 647
MaeIII GTNAC 1 cut(s) 199
MalI GATC 8 cut(s) 279, 413, 501, 506, 639, 656, 770, 884
MbiI CCGCTC 2 cut(s) 188, 223
MboI GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
MflI RGATCY 2 cut(s) 277, 654
MhlI GDGCHC 1 cut(s) 632
MluCI AATT 7 cut(s) 160, 527, 751, 940, 949, 1080, 1117
Mly113I GGCGCC 3 cut(s) 332, 562, 702
MlyI GAGTC 1 cut(s) 362
MmeI TCCRAC 1 cut(s) 1155
MreI CGCCGGCG 2 cut(s) 238, 835
MroNI GCCGGC 4 cut(s) 238, 349, 835, 868
MslI CAYNNNNRTG 1 cut(s) 900
MspA1I CMGCKG 9 cut(s) 292, 325, 388, 523, 544, 606, 788, 829, 1087
MspR9I CCNGG 2 cut(s) 215, 311
MvnI CGCG 3 cut(s) 209, 236, 292
NaeI GCCGGC 4 cut(s) 240, 351, 837, 870
NarI GGCGCC 3 cut(s) 332, 562, 702
NciI CCSGG 2 cut(s) 215, 311
NdeII GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
NgoMIV GCCGGC 4 cut(s) 238, 349, 835, 868
NlaIV GGNNCC 7 cut(s) 6, 219, 279, 333, 563, 703, 876
NmeAIII GCCGAG 1 cut(s) 879
NmuCI GTSAC 1 cut(s) 199
NotI GCGGCCGC 1 cut(s) 287
NspV TTCGAA 1 cut(s) 1187
PaeR7I CTCGAG 1 cut(s) 370
PcsI WCGNNNNNNNCGW 7 cut(s) 87, 102, 111, 114, 117, 123, 714
PdiI GCCGGC 4 cut(s) 240, 351, 837, 870
PfeI GAWTC 4 cut(s) 298, 892, 1075, 1210
PflFI GACNNNGTC 3 cut(s) 80, 107, 311
PflMI CCANNNNNTGG 1 cut(s) 547
PleI GAGTC 1 cut(s) 362
PluTI GGCGCC 3 cut(s) 335, 565, 705
PpsI GAGTC 1 cut(s) 362
PspN4I GGNNCC 7 cut(s) 6, 219, 279, 333, 563, 703, 876
PspPI GGNCC 5 cut(s) 4, 217, 1043, 1100, 1242
PspXI VCTCGAGB 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 2 cut(s) 277, 654
PsyI GACNNNGTC 3 cut(s) 80, 107, 311
PvuII CAGCTG 2 cut(s) 523, 544
RsaI GTAC 2 cut(s) 305, 578
RsaNI GTAC 2 cut(s) 304, 577
RseI CAYNNNNRTG 1 cut(s) 900
SacII CCGCGG 1 cut(s) 293
SalI GTCGAC 1 cut(s) 110
Sau3AI GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
Sau96I GGNCC 5 cut(s) 4, 217, 1043, 1100, 1242
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 215, 311
SduI GDGCHC 1 cut(s) 632
SfoI GGCGCC 3 cut(s) 333, 563, 703
Sfr274I CTCGAG 1 cut(s) 370
Sfr303I CCGCGG 1 cut(s) 293
SfuI TTCGAA 1 cut(s) 1187
SgrAI CRCCGGYG 2 cut(s) 238, 835
SgrBI CCGCGG 1 cut(s) 293
SgrDI CGTCGACG 1 cut(s) 110
SinI GGWCC 1 cut(s) 1242
SlaI CTCGAG 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 900
SmlI CTYRAG 2 cut(s) 370, 658
SmoI CTYRAG 2 cut(s) 370, 658
Sse9I AATT 7 cut(s) 160, 527, 751, 940, 949, 1080, 1117
SspDI GGCGCC 3 cut(s) 331, 561, 701
SspMI CTAG 1 cut(s) 1050
StyD4I CCNGG 2 cut(s) 213, 309
TaaI ACNGT 3 cut(s) 199, 205, 982
TaiI ACGT 4 cut(s) 84, 111, 129, 650
TaqI TCGA 7 cut(s) 111, 371, 414, 512, 765, 1106, 1187
TasI AATT 7 cut(s) 160, 527, 751, 940, 949, 1080, 1117
TfiI GAWTC 4 cut(s) 298, 892, 1075, 1210
TscAI CASTG 1 cut(s) 1154
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 3 cut(s) 319, 841, 1159
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 2 cut(s) 413, 791
TspRI CASTG 1 cut(s) 1154
Tth111I GACNNNGTC 3 cut(s) 80, 107, 311
Van91I CCANNNNNTGG 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 1242
XapI RAATTY 2 cut(s) 527, 1117
XbaI TCTAGA 1 cut(s) 1049
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 1 cut(s) 1050
ZraI GACGTC 3 cut(s) 82, 109, 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.