Rroxscaffold_6G00429330

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017098
Physical Location & Seq
Reverse (-)
49627174 .. 49629175
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00429330.1

Sequence Viewer

Length: 1074 bp
ATGGCCCCCGACCAAGACGACGCCGTTCTCCCCGAGGGCGACCTCTCCTCCTCCTCCCAAGACGACGAGGAAGAAGACGACGTCGTTGACGAGGACGATGATGACGACGTCGACGACGACGATGACGTCATCAACTCCGCCTCTCCTTCCTCCACCTCCAATTCCCTCCCCGTCGCCATAGCCACCGCTGCTCCCACCGTCACCGTCGCGCTCCCGGCCCCGCTCGCCACAACCGCGCCGGCGGCGGCCACGACGGCGGTGGTTTCCGAGGGTTTGGATCCGAAGCGGCCGCGGACGATTCAGTACGACCCGGTCTTGCTGCCGCTGGCGGCGCCGGAGGAGAAGAAGCCGGCGGCGGCGCTGGACGACTCGAGGAGGCTGTTCCAGCGGCTGTGGACGGACGAGGACGAGATCGAGCTACTGCAAGGGTTTCTGGACTACACGACGCAGAGAGGGAACAAAGGCGGGTCGCACCAAAACGACACCGCTTTGTTCTACGATCAGATCAAGTCGAAGCTCCAGCTGGAATTCAACAAGAACCAGCTGGTGGAGAAGCTCCGGCGCCTGAAGAAGAAGTACCGGAATGTTCTCAACAAAATCGCCAGCGGCAAAGAGGTCAGCTTCAAGAGCCCTCACGATCAGGCCACGTTTGAGATCTCAAGGAAGATATGGAGCAACATCGGCCGTATAGGGGTTGGTGGCGCCGACGACAACGACGACGAAGACCCTAACCCTAACCCTAACAATCCCAATTTCAACCATTTCGAGATCAAGACGGAAGAGGCCGCTGGATTTCAGAAGTCCACGCCAAAATCAAGGAAGCGCCAGCGGCCGCCGGCGCAGCCGAAGACGCCGAGTTTCGTTGAGCCGGCGGTGCCGGTGATCAAGGAGAATCATCACAATGTGAACAATGGCGGTGGCGTTGGTGGTGGTGGGAGTAATTGTGGAAATTGTAATGTTCAGGGGATGATTGAGGAGACCGTGAAGAGTTGTTTGTCTCCTGTGTTGAGGGAGTTGATGAGCAGTTCAATGGGGGAGGAGGGCCGTCTAGAGGGTTTGGAGGGCTGTCGTTGA

Protein Analysis

357

Amino Acids

38.79

Weight (kDa)

4.63

Isoelectric Point (pI)

59.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 128 - 225 2.1e-32 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20613
rosa_chinensis RchiOBHm_Chr3g0452191
rosa_laevigata RLG00000013009 RLG00000025619
rosa_multiflora Rmu_sc0021298.1_g000001
rosa_roxburghii Rroxscaffold_164G00435970 Rroxscaffold_6G00429330
rosa_rugosa Rorug02G0639100
rosa_samantha Rh3AG040900 Rh3BG042500 Rh3CG041000 Rh3DG041900
rosa_wichuraiana Rw3G003130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AatII GACGTC 3 cut(s) 84, 111, 129
AccB1I GGYRCC 4 cut(s) 331, 561, 701, 874
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 111
AccII CGCG 3 cut(s) 209, 236, 292
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 4 cut(s) 246, 287, 682, 830
AcsI RAATTY 1 cut(s) 527
AcuI CTGAAG 1 cut(s) 587
AcyI GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
AdeI CACNNNGTG 1 cut(s) 904
AfaI GTAC 2 cut(s) 305, 578
AfiI CCNNNNNNNGG 3 cut(s) 547, 691, 1051
AgsI TTSAA 4 cut(s) 532, 625, 757, 1029
AluBI AGCT 6 cut(s) 418, 517, 523, 544, 556, 621
AluI AGCT 6 cut(s) 418, 517, 523, 544, 556, 621
Alw26I GTCTC 2 cut(s) 971, 1002
AlwI GGATC 2 cut(s) 272, 285
AlwNI CAGNNNCTG 1 cut(s) 391
Ama87I CYCGRG 2 cut(s) 32, 370
AoxI GGCC 9 cut(s) 3, 216, 246, 287, 642, 682, 783, 830, 1042
ApeKI GCWGC 3 cut(s) 188, 319, 841
ApoI RAATTY 1 cut(s) 527
AspLEI GCGC 8 cut(s) 211, 238, 334, 361, 564, 704, 825, 841
AspS9I GGNCC 3 cut(s) 4, 217, 1042
AsuC2I CCSGG 2 cut(s) 215, 311
AsuHPI GGTGA 2 cut(s) 193, 892
AvaI CYCGRG 2 cut(s) 32, 370
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 4 cut(s) 331, 561, 701, 874
BanII GRGCYC 1 cut(s) 632
BarI GAAGNNNNNNTAC 2 cut(s) 560, 592
BbsI GAAGAC 3 cut(s) 81, 729, 854
BbvI GCAGC 3 cut(s) 175, 306, 853
BceAI ACGGC 4 cut(s) 8, 270, 669, 1029
BclI TGATCA 1 cut(s) 882
BcnI CCSGG 2 cut(s) 215, 311
BcoDI GTCTC 2 cut(s) 971, 1002
BfaI CTAG 1 cut(s) 1049
BfoI RGCGCY 5 cut(s) 335, 362, 565, 705, 826
BglI GCCNNNNNGGC 1 cut(s) 254
BglII AGATCT 1 cut(s) 654
Bme1390I CCNGG 2 cut(s) 215, 311
BmeT110I CYCGRG 2 cut(s) 32, 370
BmgT120I GGNCC 3 cut(s) 4, 217, 1042
BmiI GGNNCC 7 cut(s) 6, 219, 279, 333, 563, 703, 876
BmrFI CCNGG 2 cut(s) 215, 311
BpiI GAAGAC 3 cut(s) 81, 729, 854
BpmI CTGGAG 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 643
BpuMI CCSGG 2 cut(s) 215, 311
BsaHI GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
BsaI GGTCTC 1 cut(s) 971
BsaJI CCNNGG 3 cut(s) 33, 267, 290
BsaWI WCCGGW 1 cut(s) 579
BsaXI ACNNNNNCTCC 6 cut(s) 32, 62, 175, 205, 928, 958
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 691, 1051
Bse118I RCCGGY 5 cut(s) 238, 349, 835, 868, 877
BseDI CCNNGG 3 cut(s) 33, 267, 290
BseGI GGATG 1 cut(s) 972
BseLI CCNNNNNNNGG 3 cut(s) 547, 691, 1051
BseRI GAGGAG 7 cut(s) 37, 40, 43, 353, 388, 989, 1052
BseX3I CGGCCG 3 cut(s) 287, 682, 830
BseXI GCAGC 3 cut(s) 175, 306, 853
Bsh1236I CGCG 3 cut(s) 209, 236, 292
Bsh1285I CGRYCG 3 cut(s) 290, 685, 833
BshFI GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 832, 1044
BshNI GGYRCC 4 cut(s) 331, 561, 701, 874
BsiEI CGRYCG 3 cut(s) 290, 685, 833
BsiHKCI CYCGRG 2 cut(s) 32, 370
BslI CCNNNNNNNGG 3 cut(s) 547, 691, 1051
BsmAI GTCTC 2 cut(s) 971, 1002
BsnI GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 832, 1044
Bso31I GGTCTC 1 cut(s) 971
BsoBI CYCGRG 2 cut(s) 32, 370
Bsp1286I GDGCHC 1 cut(s) 632
Bsp143I GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
BspANI GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 832, 1044
BspFNI CGCG 3 cut(s) 209, 236, 292
BspLI GGNNCC 7 cut(s) 6, 219, 279, 333, 563, 703, 876
BspPI GGATC 2 cut(s) 272, 285
BspT107I GGYRCC 4 cut(s) 331, 561, 701, 874
BspTNI GGTCTC 1 cut(s) 971
BsrBI CCGCTC 1 cut(s) 223
BsrFI RCCGGY 5 cut(s) 238, 349, 835, 868, 877
BssAI RCCGGY 5 cut(s) 238, 349, 835, 868, 877
BssECI CCNNGG 3 cut(s) 33, 267, 290
BssMI GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
BssNI GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
Bst4CI ACNGT 3 cut(s) 199, 205, 982
Bst6I CTCTTC 2 cut(s) 774, 980
BstACI GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
BstC8I GCNNGC 8 cut(s) 225, 240, 327, 351, 604, 827, 837, 870
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 1 cut(s) 972
BstFNI CGCG 3 cut(s) 209, 236, 292
BstH2I RGCGCY 5 cut(s) 335, 362, 565, 705, 826
BstHHI GCGC 8 cut(s) 211, 238, 334, 361, 564, 704, 825, 841
BstKTI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
BstMAI GTCTC 2 cut(s) 971, 1002
BstMBI GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
BstMCI CGRYCG 3 cut(s) 290, 685, 833
BstSCI CCNGG 2 cut(s) 213, 309
BstUI CGCG 3 cut(s) 209, 236, 292
BstV1I GCAGC 3 cut(s) 175, 306, 853
BstV2I GAAGAC 3 cut(s) 81, 729, 854
BstX2I RGATCY 2 cut(s) 277, 654
BstYI RGATCY 2 cut(s) 277, 654
BstZI CGGCCG 3 cut(s) 287, 682, 830
BsuRI GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 832, 1044
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 1 cut(s) 972
Cac8I GCNNGC 8 cut(s) 225, 240, 327, 351, 604, 827, 837, 870
CaiI CAGNNNCTG 1 cut(s) 391
CciNI GCGGCCGC 2 cut(s) 287, 830
CfoI GCGC 8 cut(s) 211, 238, 334, 361, 564, 704, 825, 841
Cfr10I RCCGGY 5 cut(s) 238, 349, 835, 868, 877
Cfr13I GGNCC 3 cut(s) 4, 217, 1042
Cfr42I CCGCGG 1 cut(s) 293
CseI GACGC 3 cut(s) 29, 454, 859
Csp6I GTAC 2 cut(s) 304, 577
CspCI CAANNNNNGTGG 2 cut(s) 898, 933
CviQI GTAC 2 cut(s) 304, 577
DinI GGCGCC 3 cut(s) 333, 563, 703
DpnI GATC 8 cut(s) 279, 413, 501, 506, 639, 656, 770, 884
DpnII GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
DraIII CACNNNGTG 1 cut(s) 904
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 4 cut(s) 246, 287, 682, 830
EagI CGGCCG 3 cut(s) 287, 682, 830
Eam1104I CTCTTC 2 cut(s) 774, 980
EarI CTCTTC 2 cut(s) 774, 980
EciI GGCGGA 1 cut(s) 127
EclXI CGGCCG 3 cut(s) 287, 682, 830
Eco24I GRGCYC 1 cut(s) 632
Eco31I GGTCTC 1 cut(s) 971
Eco52I CGGCCG 3 cut(s) 287, 682, 830
Eco57I CTGAAG 1 cut(s) 587
Eco88I CYCGRG 2 cut(s) 32, 370
EcoRI GAATTC 1 cut(s) 527
EcoT38I GRGCYC 1 cut(s) 632
EgeI GGCGCC 3 cut(s) 333, 563, 703
EheI GGCGCC 3 cut(s) 333, 563, 703
FaiI YATR 3 cut(s) 179, 670, 689
FauI CCCGC 2 cut(s) 228, 458
FbaI TGATCA 1 cut(s) 882
FblI GTMKAC 1 cut(s) 111
FokI GGATG 1 cut(s) 979
FriOI GRGCYC 1 cut(s) 632
FspBI CTAG 1 cut(s) 1049
GlaI GCGC 8 cut(s) 210, 237, 333, 360, 563, 703, 824, 840
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 5 cut(s) 335, 362, 565, 705, 826
HaeIII GGCC 9 cut(s) 5, 218, 248, 289, 644, 684, 785, 832, 1044
HgaI GACGC 3 cut(s) 29, 454, 859
HhaI GCGC 8 cut(s) 211, 238, 334, 361, 564, 704, 825, 841
Hin1I GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
Hin6I GCGC 8 cut(s) 209, 236, 332, 359, 562, 702, 823, 839
HinP1I GCGC 8 cut(s) 209, 236, 332, 359, 562, 702, 823, 839
HincII GTYRAC 2 cut(s) 88, 112
HindII GTYRAC 2 cut(s) 88, 112
HinfI GANTC 3 cut(s) 298, 368, 892
HphI GGTGA 2 cut(s) 193, 892
Hpy166II GTNNAC 5 cut(s) 88, 112, 396, 804, 907
Hpy188I TCNGA 4 cut(s) 268, 282, 504, 798
Hpy188III TCNNGA 6 cut(s) 434, 625, 635, 766, 772, 1049
Hpy8I GTNNAC 5 cut(s) 88, 112, 396, 804, 907
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 3 cut(s) 199, 205, 982
HpyCH4IV ACGT 4 cut(s) 81, 108, 126, 647
HpyCH4V TGCA 1 cut(s) 424
HpySE526I ACGT 4 cut(s) 81, 108, 126, 647
Hsp92I GRCGYC 8 cut(s) 21, 81, 108, 126, 332, 562, 702, 851
HspAI GCGC 8 cut(s) 209, 236, 332, 359, 562, 702, 823, 839
KasI GGCGCC 3 cut(s) 331, 561, 701
KroI GCCGGC 4 cut(s) 238, 349, 835, 868
KroNI GCCGGC 4 cut(s) 240, 351, 837, 870
Ksp22I TGATCA 1 cut(s) 882
KspI CCGCGG 1 cut(s) 293
Kzo9I GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
LmnI GCTCC 5 cut(s) 196, 216, 522, 561, 672
Lsp1109I GCAGC 3 cut(s) 175, 306, 853
MaeI CTAG 1 cut(s) 1049
MaeII ACGT 4 cut(s) 81, 108, 126, 647
MaeIII GTNAC 1 cut(s) 199
MalI GATC 8 cut(s) 279, 413, 501, 506, 639, 656, 770, 884
MbiI CCGCTC 1 cut(s) 223
MboI GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
MflI RGATCY 2 cut(s) 277, 654
MhlI GDGCHC 1 cut(s) 632
MluCI AATT 5 cut(s) 160, 527, 751, 940, 949
Mly113I GGCGCC 3 cut(s) 332, 562, 702
MlyI GAGTC 1 cut(s) 362
MreI CGCCGGCG 2 cut(s) 238, 835
MroNI GCCGGC 4 cut(s) 238, 349, 835, 868
MslI CAYNNNNRTG 1 cut(s) 900
MspA1I CMGCKG 9 cut(s) 188, 292, 325, 388, 523, 544, 606, 788, 829
MspR9I CCNGG 2 cut(s) 215, 311
MvnI CGCG 3 cut(s) 209, 236, 292
NaeI GCCGGC 4 cut(s) 240, 351, 837, 870
NarI GGCGCC 3 cut(s) 332, 562, 702
NciI CCSGG 2 cut(s) 215, 311
NdeII GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
NgoMIV GCCGGC 4 cut(s) 238, 349, 835, 868
NlaIV GGNNCC 7 cut(s) 6, 219, 279, 333, 563, 703, 876
NmeAIII GCCGAG 1 cut(s) 879
NmuCI GTSAC 1 cut(s) 199
NotI GCGGCCGC 2 cut(s) 287, 830
PaeR7I CTCGAG 1 cut(s) 370
PcsI WCGNNNNNNNCGW 7 cut(s) 87, 102, 111, 114, 117, 123, 714
PdiI GCCGGC 4 cut(s) 240, 351, 837, 870
PfeI GAWTC 2 cut(s) 298, 892
PflFI GACNNNGTC 3 cut(s) 80, 107, 311
PflMI CCANNNNNTGG 1 cut(s) 547
PleI GAGTC 1 cut(s) 362
PluTI GGCGCC 3 cut(s) 335, 565, 705
PpsI GAGTC 1 cut(s) 362
PspN4I GGNNCC 7 cut(s) 6, 219, 279, 333, 563, 703, 876
PspPI GGNCC 3 cut(s) 4, 217, 1042
PspXI VCTCGAGB 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 2 cut(s) 277, 654
PsyI GACNNNGTC 3 cut(s) 80, 107, 311
PvuII CAGCTG 2 cut(s) 523, 544
RsaI GTAC 2 cut(s) 305, 578
RsaNI GTAC 2 cut(s) 304, 577
RseI CAYNNNNRTG 1 cut(s) 900
SacII CCGCGG 1 cut(s) 293
SalI GTCGAC 1 cut(s) 110
Sau3AI GATC 8 cut(s) 277, 411, 499, 504, 637, 654, 768, 882
Sau96I GGNCC 3 cut(s) 4, 217, 1042
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 215, 311
SduI GDGCHC 1 cut(s) 632
SfoI GGCGCC 3 cut(s) 333, 563, 703
Sfr274I CTCGAG 1 cut(s) 370
Sfr303I CCGCGG 1 cut(s) 293
SgrAI CRCCGGYG 2 cut(s) 238, 835
SgrBI CCGCGG 1 cut(s) 293
SgrDI CGTCGACG 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 900
SmlI CTYRAG 2 cut(s) 370, 658
SmoI CTYRAG 2 cut(s) 370, 658
Sse9I AATT 5 cut(s) 160, 527, 751, 940, 949
SspDI GGCGCC 3 cut(s) 331, 561, 701
SspMI CTAG 1 cut(s) 1049
StyD4I CCNGG 2 cut(s) 213, 309
TaaI ACNGT 3 cut(s) 199, 205, 982
TaiI ACGT 4 cut(s) 84, 111, 129, 650
TaqI TCGA 5 cut(s) 111, 371, 414, 512, 765
TasI AATT 5 cut(s) 160, 527, 751, 940, 949
TfiI GAWTC 2 cut(s) 298, 892
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 3 cut(s) 188, 319, 841
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 2 cut(s) 413, 791
Tth111I GACNNNGTC 3 cut(s) 80, 107, 311
Van91I CCANNNNNTGG 1 cut(s) 547
XapI RAATTY 1 cut(s) 527
XbaI TCTAGA 1 cut(s) 1048
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 1 cut(s) 1049
ZraI GACGTC 3 cut(s) 82, 109, 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.