Rh3CG041000

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0017098
Physical Location & Seq
Reverse (-)
2758634 .. 2759905
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG041000.1

Sequence Viewer

Length: 1272 bp
ATGGCCCCCGACCAAGACGACGCCGTTCTGCCCGAGGGCGACCTCTCCTCCTCCTCCCAAGACGACGAGGAAGAAGACGTCGATGTTGACGAGGACGAGGACGACGACGTTGACGACGACGATGACGTCATCAACTCCGCCTCTCCTTCCTCCACCTCCAATTCCCTCCCCGTCGCCATAGCCACCGCTGCTCCCACCGTCACCGTCGCGCTCCCGGCTCCGCACGCCACCACCGCGCCGGCGACGACCACGACGACGGCCGTGATTTCCGAGGGTTTGGATCCGAAGCGGCCGCGGACGTTTCAGTACGACCCGGTCTTGCTGCCGCTGGCGGCGCCCGAGGAGAAGAAGCCGGCGGCGGCGCTGGACGACTCGAGGAGGCTGTTCCAGCGGCTGTGGACGGACGAGGACGAGATCGAGCTGCTGCAAGGGTTTCTGGACTACACGACGCAGAGAGGGAACAAAGGCGGGTCGCACCAAAACGACACCGCTTTGTTCTACGATCAGATCAAGTCGAAGCTCCAGCTGGAATTCAACAAGAACCAGCTGGTGGAGAAGCTCCGGCGCCTGAAGAAGAAGTACCGGAATGTTCTCAACAAAATCGCCAGCGGCAAAGAGGTCAGCTTCAAGAGCCCTCACGATCAGGCCACCTTCGAGATCTCCAGGAAGATATGGAGCAACATCGGTCGTATCGGAGTTGCTGGCGCCGACGACAACGACGACGAAGACCCTAACCCTAACCCTAACAATCCCAATTTCAACCATTTCGAGATCAAGACGGAAGAGGCCGCCGGATTTCAGAAGTCCACGCCGAAATCGCGGAAGCGGCAGCGGACGCCGGCGCAGCCGAAAACGCCGAGTTTCGTCGAGCCGGCGGTGCCGGTGATCAAAGAGAATCATCACAATGTGAACAATGGCGGTGGCGTTGGTGGTGGTGGTGGTGGGAGTAATTGTGGAAATTGTAATGTTCAGGGGATGATTGAGGAGACGGTGAAGAGTTGTTTGTCTCCTGTGTTGAGGGAGTTGATGAGCAGTTCAATGGGTGGAGGAGGGCCGTCTAGAGGGTTTGGAGGGCTGTCATTGAATCCGATTCCGCTGAGCTTTGGCGGTCCTTCGATGAGTTTGAATTTTGGGGGTGGGGAAGCGGCGGATGAGCAGTGGAGGAAGCAGCAGATTTTGGAGTTGGAGGTGTATTCGAAGCGATTGGAGTTGGTGCAGAATCAGATAAAAGTGGCATTGGATGAGTTGCGGTCCACAGGAGGAGGAGGCTGA

Protein Analysis

423

Amino Acids

45.7

Weight (kDa)

4.75

Isoelectric Point (pI)

58.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 129 - 226 2.9e-32 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20613
rosa_chinensis RchiOBHm_Chr3g0452191
rosa_laevigata RLG00000013009 RLG00000025619
rosa_multiflora Rmu_sc0021298.1_g000001
rosa_roxburghii Rroxscaffold_164G00435970 Rroxscaffold_6G00429330
rosa_rugosa Rorug02G0639100
rosa_samantha Rh3AG040900 Rh3BG042500 Rh3CG041000 Rh3DG041900
rosa_wichuraiana Rw3G003130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AatII GACGTC 2 cut(s) 81, 129
AccB1I GGYRCC 4 cut(s) 334, 564, 704, 877
AccB7I CCANNNNNTGG 1 cut(s) 550
AccII CGCG 4 cut(s) 209, 236, 295, 820
AclWI GGATC 2 cut(s) 275, 288
AcoI YGGCCR 2 cut(s) 258, 290
AcsI RAATTY 2 cut(s) 530, 1126
AcuI CTGAAG 1 cut(s) 590
AcyI GRCGYC 7 cut(s) 21, 78, 126, 335, 565, 705, 836
AdeI CACNNNGTG 1 cut(s) 907
AfaI GTAC 2 cut(s) 308, 581
AfiI CCNNNNNNNGG 2 cut(s) 550, 1061
AgsI TTSAA 6 cut(s) 535, 628, 760, 1038, 1084, 1126
AjnI CCWGG 1 cut(s) 662
AluBI AGCT 7 cut(s) 421, 520, 526, 547, 559, 624, 1101
AluI AGCT 7 cut(s) 421, 520, 526, 547, 559, 624, 1101
Alw26I GTCTC 2 cut(s) 980, 1011
AlwI GGATC 2 cut(s) 275, 288
AlwNI CAGNNNCTG 1 cut(s) 394
Ama87I CYCGRG 3 cut(s) 32, 338, 373
AoxI GGCC 6 cut(s) 3, 258, 290, 645, 786, 1052
ApeKI GCWGC 7 cut(s) 188, 322, 421, 424, 829, 844, 1168
ApoI RAATTY 2 cut(s) 530, 1126
AspLEI GCGC 7 cut(s) 211, 238, 337, 364, 567, 707, 844
AspS9I GGNCC 4 cut(s) 4, 1052, 1109, 1251
AsuC2I CCSGG 2 cut(s) 215, 314
AsuHPI GGTGA 3 cut(s) 193, 895, 1003
AsuII TTCGAA 1 cut(s) 1196
AvaI CYCGRG 3 cut(s) 32, 338, 373
AvaII GGWCC 2 cut(s) 1109, 1251
BamHI GGATCC 1 cut(s) 280
BanI GGYRCC 4 cut(s) 334, 564, 704, 877
BanII GRGCYC 1 cut(s) 635
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BbsI GAAGAC 2 cut(s) 81, 732
BbvI GCAGC 7 cut(s) 175, 309, 408, 411, 841, 856, 1180
BceAI ACGGC 4 cut(s) 8, 245, 273, 1039
BciT130I CCWGG 1 cut(s) 664
BclI TGATCA 1 cut(s) 885
BcnI CCSGG 2 cut(s) 215, 314
BcoDI GTCTC 2 cut(s) 980, 1011
BfaI CTAG 1 cut(s) 1059
BfoI RGCGCY 4 cut(s) 338, 365, 568, 708
BglII AGATCT 1 cut(s) 657
BlpI GCTNAGC 1 cut(s) 1097
Bme1390I CCNGG 3 cut(s) 215, 314, 664
Bme18I GGWCC 2 cut(s) 1109, 1251
BmeT110I CYCGRG 3 cut(s) 32, 338, 373
BmgT120I GGNCC 4 cut(s) 4, 1052, 1109, 1251
BmiI GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 879
BmrFI CCNGG 3 cut(s) 215, 314, 664
BpiI GAAGAC 2 cut(s) 81, 732
BpmI CTGGAG 2 cut(s) 506, 646
Bpu1102I GCTNAGC 1 cut(s) 1097
Bpu14I TTCGAA 1 cut(s) 1196
BpuMI CCSGG 2 cut(s) 215, 314
BsaHI GRCGYC 7 cut(s) 21, 78, 126, 335, 565, 705, 836
BsaJI CCNNGG 4 cut(s) 33, 270, 293, 339
BsaWI WCCGGW 1 cut(s) 582
BsaXI ACNNNNNCTCC 6 cut(s) 32, 62, 175, 205, 937, 967
Bsc4I CCNNNNNNNGG 2 cut(s) 550, 1061
Bse118I RCCGGY 5 cut(s) 238, 352, 838, 871, 880
BseBI CCWGG 1 cut(s) 664
BseDI CCNNGG 4 cut(s) 33, 270, 293, 339
BseGI GGATG 3 cut(s) 981, 1156, 1246
BseLI CCNNNNNNNGG 2 cut(s) 550, 1061
BseMII CTCAG 1 cut(s) 1088
BseRI GAGGAG 7 cut(s) 37, 40, 43, 356, 391, 998, 1062
BseX3I CGGCCG 2 cut(s) 258, 290
BseXI GCAGC 7 cut(s) 175, 309, 408, 411, 841, 856, 1180
BsgI GTGCAG 1 cut(s) 1235
Bsh1236I CGCG 4 cut(s) 209, 236, 295, 820
Bsh1285I CGRYCG 3 cut(s) 261, 293, 688
BshFI GGCC 6 cut(s) 5, 260, 292, 647, 788, 1054
BshNI GGYRCC 4 cut(s) 334, 564, 704, 877
BsiEI CGRYCG 3 cut(s) 261, 293, 688
BsiHKCI CYCGRG 3 cut(s) 32, 338, 373
BslI CCNNNNNNNGG 2 cut(s) 550, 1061
BsmAI GTCTC 2 cut(s) 980, 1011
BsmBI CGTCTC 1 cut(s) 980
BsnI GGCC 6 cut(s) 5, 260, 292, 647, 788, 1054
BsoBI CYCGRG 3 cut(s) 32, 338, 373
Bsp119I TTCGAA 1 cut(s) 1196
Bsp1286I GDGCHC 1 cut(s) 635
Bsp143I GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
Bsp1720I GCTNAGC 1 cut(s) 1097
BspANI GGCC 6 cut(s) 5, 260, 292, 647, 788, 1054
BspCNI CTCAG 1 cut(s) 1089
BspFNI CGCG 4 cut(s) 209, 236, 295, 820
BspLI GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 879
BspPI GGATC 2 cut(s) 275, 288
BspT104I TTCGAA 1 cut(s) 1196
BspT107I GGYRCC 4 cut(s) 334, 564, 704, 877
BsrFI RCCGGY 5 cut(s) 238, 352, 838, 871, 880
BssAI RCCGGY 5 cut(s) 238, 352, 838, 871, 880
BssECI CCNNGG 4 cut(s) 33, 270, 293, 339
BssMI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
BssNI GRCGYC 7 cut(s) 21, 78, 126, 335, 565, 705, 836
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 3 cut(s) 199, 205, 991
Bst6I CTCTTC 2 cut(s) 777, 989
BstACI GRCGYC 7 cut(s) 21, 78, 126, 335, 565, 705, 836
BstBI TTCGAA 1 cut(s) 1196
BstC8I GCNNGC 8 cut(s) 225, 240, 330, 354, 607, 703, 840, 873
BstDEI CTNAG 1 cut(s) 1097
BstDSI CCRYGG 1 cut(s) 293
BstF5I GGATG 3 cut(s) 981, 1156, 1246
BstFNI CGCG 4 cut(s) 209, 236, 295, 820
BstH2I RGCGCY 4 cut(s) 338, 365, 568, 708
BstHHI GCGC 7 cut(s) 211, 238, 337, 364, 567, 707, 844
BstKTI GATC 8 cut(s) 283, 417, 505, 510, 643, 660, 774, 888
BstMAI GTCTC 2 cut(s) 980, 1011
BstMBI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
BstMCI CGRYCG 3 cut(s) 261, 293, 688
BstNI CCWGG 1 cut(s) 664
BstSCI CCNGG 3 cut(s) 213, 312, 662
BstUI CGCG 4 cut(s) 209, 236, 295, 820
BstV1I GCAGC 7 cut(s) 175, 309, 408, 411, 841, 856, 1180
BstV2I GAAGAC 2 cut(s) 81, 732
BstX2I RGATCY 2 cut(s) 280, 657
BstYI RGATCY 2 cut(s) 280, 657
BstZI CGGCCG 2 cut(s) 258, 290
BsuRI GGCC 6 cut(s) 5, 260, 292, 647, 788, 1054
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 3 cut(s) 981, 1156, 1246
BtsI GCAGTG 1 cut(s) 1163
BtsIMutI CAGTG 1 cut(s) 1163
Cac8I GCNNGC 8 cut(s) 225, 240, 330, 354, 607, 703, 840, 873
CaiI CAGNNNCTG 1 cut(s) 394
CciNI GCGGCCGC 1 cut(s) 290
CfoI GCGC 7 cut(s) 211, 238, 337, 364, 567, 707, 844
Cfr10I RCCGGY 5 cut(s) 238, 352, 838, 871, 880
Cfr13I GGNCC 4 cut(s) 4, 1052, 1109, 1251
Cfr42I CCGCGG 1 cut(s) 296
CseI GACGC 3 cut(s) 29, 457, 844
Csp6I GTAC 2 cut(s) 307, 580
CspCI CAANNNNNGTGG 2 cut(s) 901, 936
CviQI GTAC 2 cut(s) 307, 580
DdeI CTNAG 1 cut(s) 1097
DinI GGCGCC 3 cut(s) 336, 566, 706
DpnI GATC 8 cut(s) 282, 416, 504, 509, 642, 659, 773, 887
DpnII GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
DraIII CACNNNGTG 1 cut(s) 907
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 2 cut(s) 258, 290
EagI CGGCCG 2 cut(s) 258, 290
Eam1104I CTCTTC 2 cut(s) 777, 989
EarI CTCTTC 2 cut(s) 777, 989
EciI GGCGGA 2 cut(s) 127, 1163
EclXI CGGCCG 2 cut(s) 258, 290
Eco24I GRGCYC 1 cut(s) 635
Eco47I GGWCC 2 cut(s) 1109, 1251
Eco52I CGGCCG 2 cut(s) 258, 290
Eco57I CTGAAG 1 cut(s) 590
Eco88I CYCGRG 3 cut(s) 32, 338, 373
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 662
EcoT38I GRGCYC 1 cut(s) 635
EgeI GGCGCC 3 cut(s) 336, 566, 706
EheI GGCGCC 3 cut(s) 336, 566, 706
Esp3I CGTCTC 1 cut(s) 980
FaiI YATR 2 cut(s) 179, 673
FauI CCCGC 1 cut(s) 461
FbaI TGATCA 1 cut(s) 885
FokI GGATG 3 cut(s) 988, 1163, 1253
FriOI GRGCYC 1 cut(s) 635
FspBI CTAG 1 cut(s) 1059
GlaI GCGC 7 cut(s) 210, 237, 336, 363, 566, 706, 843
GsuI CTGGAG 2 cut(s) 506, 646
HaeII RGCGCY 4 cut(s) 338, 365, 568, 708
HaeIII GGCC 6 cut(s) 5, 260, 292, 647, 788, 1054
HgaI GACGC 3 cut(s) 29, 457, 844
HhaI GCGC 7 cut(s) 211, 238, 337, 364, 567, 707, 844
Hin1I GRCGYC 7 cut(s) 21, 78, 126, 335, 565, 705, 836
Hin6I GCGC 7 cut(s) 209, 236, 335, 362, 565, 705, 842
HinP1I GCGC 7 cut(s) 209, 236, 335, 362, 565, 705, 842
HincII GTYRAC 2 cut(s) 88, 112
HindII GTYRAC 2 cut(s) 88, 112
HinfI GANTC 5 cut(s) 371, 895, 1084, 1090, 1219
HphI GGTGA 3 cut(s) 193, 895, 1003
Hpy166II GTNNAC 6 cut(s) 88, 112, 399, 807, 910, 1254
Hpy188I TCNGA 7 cut(s) 271, 285, 507, 695, 801, 1089, 1224
Hpy188III TCNNGA 7 cut(s) 437, 628, 638, 655, 769, 775, 1059
Hpy8I GTNNAC 6 cut(s) 88, 112, 399, 807, 910, 1254
HpyAV CCTTC 3 cut(s) 156, 661, 1122
HpyCH4III ACNGT 3 cut(s) 199, 205, 991
HpyCH4IV ACGT 4 cut(s) 78, 108, 126, 299
HpyCH4V TGCA 2 cut(s) 427, 1216
HpyF3I CTNAG 1 cut(s) 1097
HpySE526I ACGT 4 cut(s) 78, 108, 126, 299
Hsp92I GRCGYC 7 cut(s) 21, 78, 126, 335, 565, 705, 836
HspAI GCGC 7 cut(s) 209, 236, 335, 362, 565, 705, 842
KasI GGCGCC 3 cut(s) 334, 564, 704
KroI GCCGGC 4 cut(s) 238, 352, 838, 871
KroNI GCCGGC 4 cut(s) 240, 354, 840, 873
Ksp22I TGATCA 1 cut(s) 885
KspI CCGCGG 1 cut(s) 296
Kzo9I GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
LmnI GCTCC 6 cut(s) 196, 216, 223, 525, 564, 675
Lsp1109I GCAGC 7 cut(s) 175, 309, 408, 411, 841, 856, 1180
MaeI CTAG 1 cut(s) 1059
MaeII ACGT 4 cut(s) 78, 108, 126, 299
MaeIII GTNAC 1 cut(s) 199
MalI GATC 8 cut(s) 282, 416, 504, 509, 642, 659, 773, 887
MboI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
MboII GAAGA 9 cut(s) 83, 86, 358, 583, 586, 679, 737, 794, 1006
MflI RGATCY 2 cut(s) 280, 657
MhlI GDGCHC 1 cut(s) 635
MluCI AATT 6 cut(s) 160, 530, 754, 949, 958, 1126
Mly113I GGCGCC 3 cut(s) 335, 565, 705
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 1164
MreI CGCCGGCG 2 cut(s) 238, 838
MroNI GCCGGC 4 cut(s) 238, 352, 838, 871
MslI CAYNNNNRTG 1 cut(s) 903
MspA1I CMGCKG 9 cut(s) 188, 295, 328, 391, 526, 547, 609, 832, 1096
MspR9I CCNGG 3 cut(s) 215, 314, 664
MvaI CCWGG 1 cut(s) 664
MvnI CGCG 4 cut(s) 209, 236, 295, 820
NaeI GCCGGC 4 cut(s) 240, 354, 840, 873
NarI GGCGCC 3 cut(s) 335, 565, 705
NciI CCSGG 2 cut(s) 215, 314
NdeII GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
NgoMIV GCCGGC 4 cut(s) 238, 352, 838, 871
NlaIV GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 879
NmeAIII GCCGAG 1 cut(s) 882
NmuCI GTSAC 1 cut(s) 199
NotI GCGGCCGC 1 cut(s) 290
NspV TTCGAA 1 cut(s) 1196
PaeR7I CTCGAG 1 cut(s) 373
PcsI WCGNNNNNNNCGW 7 cut(s) 87, 102, 111, 114, 123, 251, 717
PdiI GCCGGC 4 cut(s) 240, 354, 840, 873
PfeI GAWTC 4 cut(s) 895, 1084, 1090, 1219
PflFI GACNNNGTC 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 550
PfoI TCCNGGA 1 cut(s) 662
PleI GAGTC 1 cut(s) 365
PluTI GGCGCC 3 cut(s) 338, 568, 708
PpsI GAGTC 1 cut(s) 365
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 879
PspPI GGNCC 4 cut(s) 4, 1052, 1109, 1251
PspXI VCTCGAGB 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 280, 657
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 2 cut(s) 526, 547
RsaI GTAC 2 cut(s) 308, 581
RsaNI GTAC 2 cut(s) 307, 580
RseI CAYNNNNRTG 1 cut(s) 903
SacII CCGCGG 1 cut(s) 296
Sau3AI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
Sau96I GGNCC 4 cut(s) 4, 1052, 1109, 1251
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 3 cut(s) 215, 314, 664
SduI GDGCHC 1 cut(s) 635
SfoI GGCGCC 3 cut(s) 336, 566, 706
Sfr274I CTCGAG 1 cut(s) 373
Sfr303I CCGCGG 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 1196
SgrAI CRCCGGYG 2 cut(s) 238, 838
SgrBI CCGCGG 1 cut(s) 296
SinI GGWCC 2 cut(s) 1109, 1251
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 903
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 6 cut(s) 160, 530, 754, 949, 958, 1126
SspDI GGCGCC 3 cut(s) 334, 564, 704
SspMI CTAG 1 cut(s) 1059
StyD4I CCNGG 3 cut(s) 213, 312, 662
TaaI ACNGT 3 cut(s) 199, 205, 991
TaiI ACGT 4 cut(s) 81, 111, 129, 302
TaqI TCGA 9 cut(s) 81, 374, 417, 515, 654, 768, 867, 1115, 1196
TaqII GACCGA 1 cut(s) 674
TasI AATT 6 cut(s) 160, 530, 754, 949, 958, 1126
TfiI GAWTC 4 cut(s) 895, 1084, 1090, 1219
TscAI CASTG 1 cut(s) 1163
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 7 cut(s) 188, 322, 421, 424, 829, 844, 1168
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 2 cut(s) 416, 794
TspRI CASTG 1 cut(s) 1163
Tth111I GACNNNGTC 1 cut(s) 314
Van91I CCANNNNNTGG 1 cut(s) 550
VpaK11BI GGWCC 2 cut(s) 1109, 1251
XapI RAATTY 2 cut(s) 530, 1126
XbaI TCTAGA 1 cut(s) 1058
XhoI CTCGAG 1 cut(s) 373
XspI CTAG 1 cut(s) 1059
ZraI GACGTC 2 cut(s) 79, 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.