RLG00000013009

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017098
Physical Location & Seq
Forward (+)
24835060 .. 24836019
960 bp
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UTR
Exon/CDS
Intron
RLM00000013009

Sequence Viewer

Length: 489 bp
ATGATAGAGAAAATAAAGGGGATCAAACTGAGAGAAAAGAGAAGAGGAGAGACATGGTCAAATAAAAGGCTTGGAACGCTGGTTGGTGGCAAGTTGGCATCGTCCCACTGCCATGGCTCCATTGGTCCCTCACCCGGAGGGGAGGAAGCCTCTTACTACGAGGAAGAATACGACGTCGTTGACGAGGACGAGGTCGATGACTCTGCCTCTCCTTTCTCCACCTTTGATTCCCTCCCCGTCGACATAGCCACCGTCACCGTTGCGCTCCATGCTCCGCTTACCACCACCGAGCCGGCGACGACCACGATTCAGTACAACCCATTCTTGCTGCTGCAAGAGGCGCTAAAGGAGAAGAAGCCGGCAACGGCGCTGGACGACTCGAGGAGGCTATTCCAGCGGCTGTGGACAGACGAGGACGAGATCAAGTTGCTGCAAGGGTTTTTGGACTACACAACACAGAGGGGGAACCAAAGCAGGCCCAGTGCTTGA

Protein Analysis

163

Amino Acids

18.05

Weight (kDa)

4.92

Isoelectric Point (pI)

61.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 131 - 158 3.8e-07 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20613
rosa_chinensis RchiOBHm_Chr3g0452191
rosa_laevigata RLG00000013009 RLG00000025619
rosa_multiflora Rmu_sc0021298.1_g000001
rosa_roxburghii Rroxscaffold_164G00435970 Rroxscaffold_6G00429330
rosa_rugosa Rorug02G0639100
rosa_samantha Rh3AG040900 Rh3BG042500 Rh3CG041000 Rh3DG041900
rosa_wichuraiana Rw3G003130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 240
AciI CCGC 2 cut(s) 275, 397
AclWI GGATC 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 134
Alw26I GTCTC 1 cut(s) 44
AlwI GGATC 1 cut(s) 29
AlwNI CAGNNNCTG 1 cut(s) 400
Ama87I CYCGRG 1 cut(s) 379
AoxI GGCC 1 cut(s) 476
ApeKI GCWGC 3 cut(s) 328, 331, 430
AspLEI GCGC 3 cut(s) 265, 343, 370
AspS9I GGNCC 2 cut(s) 125, 477
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 2 cut(s) 123, 247
AvaI CYCGRG 1 cut(s) 379
AvaII GGWCC 1 cut(s) 125
BbvI GCAGC 3 cut(s) 315, 318, 417
BceAI ACGGC 1 cut(s) 381
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 44
BfoI RGCGCY 2 cut(s) 344, 371
BisI GCNGC 4 cut(s) 329, 332, 398, 431
BlsI GCNGC 4 cut(s) 330, 333, 399, 432
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 125, 477
BmiI GGNNCC 3 cut(s) 118, 127, 467
BmrFI CCNGG 1 cut(s) 135
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 107
BmuI ACTGGG 1 cut(s) 474
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpuMI CCSGG 1 cut(s) 135
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 112
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse118I RCCGGY 2 cut(s) 292, 358
Bse1I ACTGG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 112
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 1 cut(s) 480
BseRI GAGGAG 2 cut(s) 60, 397
BseXI GCAGC 3 cut(s) 315, 318, 417
BshFI GGCC 1 cut(s) 478
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 3 cut(s) 135, 293, 359
BslFI GGGAC 2 cut(s) 88, 111
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 44
BsmFI GGGAC 2 cut(s) 88, 111
BsnI GGCC 1 cut(s) 478
BsoBI CYCGRG 1 cut(s) 379
Bsp143I GATC 2 cut(s) 21, 420
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 2 cut(s) 275, 397
BspANI GGCC 1 cut(s) 478
BspCNI CTCAG 1 cut(s) 21
BspLI GGNNCC 3 cut(s) 118, 127, 467
BspPI GGATC 1 cut(s) 29
BsrFI RCCGGY 2 cut(s) 292, 358
BsrI ACTGG 1 cut(s) 480
BssAI RCCGGY 2 cut(s) 292, 358
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 2 cut(s) 21, 420
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 2 cut(s) 253, 259
Bst6I CTCTTC 1 cut(s) 37
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 3 cut(s) 294, 360, 476
BstDEI CTNAG 1 cut(s) 29
BstDSI CCRYGG 1 cut(s) 112
BstH2I RGCGCY 2 cut(s) 344, 371
BstHHI GCGC 3 cut(s) 265, 343, 370
BstKTI GATC 2 cut(s) 24, 423
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 2 cut(s) 21, 420
BstMWI GCNNNNNNNGC 4 cut(s) 76, 269, 340, 394
BstSCI CCNGG 1 cut(s) 133
BstV1I GCAGC 3 cut(s) 315, 318, 417
BstXI CCANNNNNNTGG 1 cut(s) 113
BsuRI GGCC 1 cut(s) 478
BtgI CCRYGG 1 cut(s) 112
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 2 cut(s) 106, 487
Cac8I GCNNGC 3 cut(s) 294, 360, 476
CaiI CAGNNNCTG 1 cut(s) 400
CfoI GCGC 3 cut(s) 265, 343, 370
Cfr10I RCCGGY 2 cut(s) 292, 358
Cfr13I GGNCC 2 cut(s) 125, 477
Csp6I GTAC 1 cut(s) 313
CviAII CATG 3 cut(s) 54, 113, 269
CviJI RGCY 9 cut(s) 70, 117, 149, 248, 292, 358, 388, 400, 478
CviKI_1 RGCY 9 cut(s) 70, 117, 149, 248, 292, 358, 388, 400, 478
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 29
DpnI GATC 2 cut(s) 23, 422
DpnII GATC 2 cut(s) 21, 420
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 3 cut(s) 57, 116, 272
FaiI YATR 4 cut(s) 55, 114, 245, 270
FaqI GGGAC 2 cut(s) 88, 111
FatI CATG 3 cut(s) 53, 112, 268
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 4 cut(s) 329, 332, 398, 431
Fsp4HI GCNGC 4 cut(s) 329, 332, 398, 431
GlaI GCGC 3 cut(s) 264, 342, 369
GluI GCNGC 4 cut(s) 329, 332, 398, 431
HaeII RGCGCY 2 cut(s) 344, 371
HaeIII GGCC 1 cut(s) 478
HapII CCGG 3 cut(s) 135, 293, 359
HhaI GCGC 3 cut(s) 265, 343, 370
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 3 cut(s) 57, 116, 272
Hin6I GCGC 3 cut(s) 263, 341, 368
HinP1I GCGC 3 cut(s) 263, 341, 368
HincII GTYRAC 2 cut(s) 181, 241
HindII GTYRAC 2 cut(s) 181, 241
HinfI GANTC 4 cut(s) 200, 227, 307, 377
HpaII CCGG 3 cut(s) 135, 293, 359
HphI GGTGA 2 cut(s) 123, 247
Hpy166II GTNNAC 3 cut(s) 181, 241, 405
Hpy8I GTNNAC 3 cut(s) 181, 241, 405
Hpy99I CGWCG 4 cut(s) 176, 179, 242, 301
HpyCH4III ACNGT 2 cut(s) 253, 259
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 2 cut(s) 334, 433
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 269, 340, 394
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 3 cut(s) 57, 116, 272
HspAI GCGC 3 cut(s) 263, 341, 368
KroI GCCGGC 2 cut(s) 292, 358
KroNI GCCGGC 2 cut(s) 294, 360
Kzo9I GATC 2 cut(s) 21, 420
LmnI GCTCC 3 cut(s) 122, 270, 277
LpnPI CCDG 7 cut(s) 65, 148, 306, 356, 372, 407, 460
Lsp1109I GCAGC 3 cut(s) 315, 318, 417
LweI GCATC 1 cut(s) 107
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 253
MalI GATC 2 cut(s) 23, 422
MboI GATC 2 cut(s) 21, 420
MboII GAAGA 3 cut(s) 54, 176, 364
MlyI GAGTC 2 cut(s) 194, 371
MroNI GCCGGC 2 cut(s) 292, 358
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 397
MspI CCGG 3 cut(s) 135, 293, 359
MspR9I CCNGG 1 cut(s) 135
MwoI GCNNNNNNNGC 4 cut(s) 76, 269, 340, 394
NaeI GCCGGC 2 cut(s) 294, 360
NciI CCSGG 1 cut(s) 135
NcoI CCATGG 1 cut(s) 112
NdeII GATC 2 cut(s) 21, 420
NgoMIV GCCGGC 2 cut(s) 292, 358
NlaIII CATG 3 cut(s) 57, 116, 272
NlaIV GGNNCC 3 cut(s) 118, 127, 467
NmuCI GTSAC 1 cut(s) 253
PaeR7I CTCGAG 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PdiI GCCGGC 2 cut(s) 294, 360
PfeI GAWTC 2 cut(s) 227, 307
PflFI GACNNNGTC 2 cut(s) 55, 191
PkrI GCNGC 4 cut(s) 330, 333, 399, 432
PleI GAGTC 2 cut(s) 194, 371
PpsI GAGTC 2 cut(s) 194, 371
PspN4I GGNNCC 3 cut(s) 118, 127, 467
PspPI GGNCC 2 cut(s) 125, 477
PspXI VCTCGAGB 1 cut(s) 379
PstNI CAGNNNCTG 1 cut(s) 400
PsyI GACNNNGTC 2 cut(s) 55, 191
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 111
SalI GTCGAC 1 cut(s) 239
SatI GCNGC 4 cut(s) 329, 332, 398, 431
Sau3AI GATC 2 cut(s) 21, 420
Sau96I GGNCC 2 cut(s) 125, 477
SchI GAGTC 2 cut(s) 194, 371
ScrFI CCNGG 1 cut(s) 135
SetI ASST 3 cut(s) 177, 195, 224
SfaNI GCATC 1 cut(s) 107
Sfr274I CTCGAG 1 cut(s) 379
SinI GGWCC 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
SsiI CCGC 2 cut(s) 275, 397
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 2 cut(s) 253, 259
TaiI ACGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 195, 240, 380
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 2 cut(s) 227, 307
TscAI CASTG 2 cut(s) 113, 487
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 3 cut(s) 328, 331, 430
Tsp45I GTSAC 1 cut(s) 253
TspRI CASTG 2 cut(s) 113, 487
Tth111I GACNNNGTC 2 cut(s) 55, 191
VpaK11BI GGWCC 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 240
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.