Rw3G003130

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017098
Physical Location & Seq
Reverse (-)
2744787 .. 2747371
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003130.1

Sequence Viewer

Length: 990 bp
ATGGCCCCCGACCAAGACGACGCCGTTCTCCCCGAGGGCGACCTCTCCTCCTCCTCCCAAGACGACGAGGAAGAAGACGTCGACGTTGACGAGGACGAGGACGACGACGTCGACGACGACGATGACGTCATCAATTCCGCCTCTCCTTCCTCCACCTCCAATTCCCTCCCCGTCGCCATAGCCACCGCTGCTCCCACCGTCACCGTCGCGCTCCCGGCTCCTCACGCCACCACCGCTCCGGCGACGACTACGACGACGGCCGTGCTTTCCGAGGGTTTGGATCCGAAGCGGCCGCGGACGATTCAGTACGACCCGGTCTTGCTGCCGCTGGCGGCGCCGGAGGAGAAGAAGCCGGCGGCGGCGCTGGACGACTCGAGGAGGCTGTTCCAGCGGCTGTGGACGGACGAGGACGAGATCGAGCTGCTGCAAGGGTTTCTGGACTACACGACGCAGAGAGGGAACAAAGGCGGGTCGCACCAAAACGACACCGCTTTGTTCTACGATCAGATCAAGTCGAAGCTCCAGCTGGAATTCAACAAGAACCAGCTGGTGGAGAAGCTCCGGCGCCTGAAGAAGAAGTACCGGAATGTTCTCAACAAAATCGCCAGCGGCAAAGAGGTCAGCTTCAAGAGCCCTCACGATCAGGCCACCTTCGAGATCTCCAGGAAGATATGGAGCAACATCGGCCGTATCGGAGTTGCTGGCGCCGACGACAACGACGACGAAGACCCTAACCCTAACCCCAACAATCCCAATTTCAACCATTTCGAGATCAAGACGGAAGAGGCCGCCGGATTTCAGAAGTCCACGCCGAAATCGCGGAAGCGGCAGCGGACGCCGGCGCAGCCGAAAACGCCGAGTTTCGTCGAGCCGGCGGTGCCGGTGATCAAAGAGAATCATCACAATGTGAACAATGGCGGTGGCGTTGGTGTTGGTGGTGGTGGTGGTGGGGTGGGAGTAATTGTGGAAATTGTAATGTTCAGGGGATGA

Protein Analysis

329

Amino Acids

35.78

Weight (kDa)

4.7

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 129 - 226 1.8e-32 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20613
rosa_chinensis RchiOBHm_Chr3g0452191
rosa_laevigata RLG00000013009 RLG00000025619
rosa_multiflora Rmu_sc0021298.1_g000001
rosa_roxburghii Rroxscaffold_164G00435970 Rroxscaffold_6G00429330
rosa_rugosa Rorug02G0639100
rosa_samantha Rh3AG040900 Rh3BG042500 Rh3CG041000 Rh3DG041900
rosa_wichuraiana Rw3G003130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 107, 125
AatII GACGTC 3 cut(s) 81, 111, 129
AccB1I GGYRCC 4 cut(s) 334, 564, 704, 877
AccB7I CCANNNNNTGG 1 cut(s) 550
AccBSI CCGCTC 1 cut(s) 236
AccI GTMKAC 2 cut(s) 81, 111
AccII CGCG 3 cut(s) 209, 295, 820
AclWI GGATC 2 cut(s) 275, 288
AcoI YGGCCR 3 cut(s) 258, 290, 685
AcsI RAATTY 1 cut(s) 530
AcuI CTGAAG 1 cut(s) 590
AcyI GRCGYC 8 cut(s) 21, 78, 108, 126, 335, 565, 705, 836
AdeI CACNNNGTG 1 cut(s) 907
AfaI GTAC 2 cut(s) 308, 581
AfiI CCNNNNNNNGG 1 cut(s) 550
AgsI TTSAA 3 cut(s) 535, 628, 760
AjnI CCWGG 1 cut(s) 662
AluBI AGCT 6 cut(s) 421, 520, 526, 547, 559, 624
AluI AGCT 6 cut(s) 421, 520, 526, 547, 559, 624
AlwI GGATC 2 cut(s) 275, 288
AlwNI CAGNNNCTG 1 cut(s) 394
Ama87I CYCGRG 2 cut(s) 32, 373
AoxI GGCC 6 cut(s) 3, 258, 290, 645, 685, 786
ApeKI GCWGC 6 cut(s) 188, 322, 421, 424, 829, 844
ApoI RAATTY 1 cut(s) 530
AspLEI GCGC 6 cut(s) 211, 337, 364, 567, 707, 844
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 2 cut(s) 215, 314
AsuHPI GGTGA 2 cut(s) 193, 895
AvaI CYCGRG 2 cut(s) 32, 373
BamHI GGATCC 1 cut(s) 280
BanI GGYRCC 4 cut(s) 334, 564, 704, 877
BanII GRGCYC 1 cut(s) 635
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BbsI GAAGAC 2 cut(s) 81, 732
BbvI GCAGC 6 cut(s) 175, 309, 408, 411, 841, 856
BceAI ACGGC 4 cut(s) 8, 245, 273, 672
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 1 cut(s) 664
BclI TGATCA 1 cut(s) 885
BcnI CCSGG 2 cut(s) 215, 314
BfoI RGCGCY 4 cut(s) 338, 365, 568, 708
BglII AGATCT 1 cut(s) 657
Bme1390I CCNGG 3 cut(s) 215, 314, 664
BmeT110I CYCGRG 2 cut(s) 32, 373
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 879
BmrFI CCNGG 3 cut(s) 215, 314, 664
BpiI GAAGAC 2 cut(s) 81, 732
BpmI CTGGAG 2 cut(s) 506, 646
BpuMI CCSGG 2 cut(s) 215, 314
BsaHI GRCGYC 8 cut(s) 21, 78, 108, 126, 335, 565, 705, 836
BsaJI CCNNGG 3 cut(s) 33, 270, 293
BsaWI WCCGGW 1 cut(s) 582
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 175, 205, 220, 250, 948, 978
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse118I RCCGGY 4 cut(s) 352, 838, 871, 880
BseBI CCWGG 1 cut(s) 664
BseDI CCNNGG 3 cut(s) 33, 270, 293
BseLI CCNNNNNNNGG 1 cut(s) 550
BseRI GAGGAG 6 cut(s) 37, 40, 43, 210, 356, 391
BseX3I CGGCCG 3 cut(s) 258, 290, 685
BseXI GCAGC 6 cut(s) 175, 309, 408, 411, 841, 856
Bsh1236I CGCG 3 cut(s) 209, 295, 820
Bsh1285I CGRYCG 3 cut(s) 261, 293, 688
BshFI GGCC 6 cut(s) 5, 260, 292, 647, 687, 788
BshNI GGYRCC 4 cut(s) 334, 564, 704, 877
BsiEI CGRYCG 3 cut(s) 261, 293, 688
BsiHKCI CYCGRG 2 cut(s) 32, 373
BslI CCNNNNNNNGG 1 cut(s) 550
BsnI GGCC 6 cut(s) 5, 260, 292, 647, 687, 788
BsoBI CYCGRG 2 cut(s) 32, 373
Bsp1286I GDGCHC 1 cut(s) 635
Bsp143I GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
BspANI GGCC 6 cut(s) 5, 260, 292, 647, 687, 788
BspFNI CGCG 3 cut(s) 209, 295, 820
BspLI GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 879
BspPI GGATC 2 cut(s) 275, 288
BspT107I GGYRCC 4 cut(s) 334, 564, 704, 877
BsrBI CCGCTC 1 cut(s) 236
BsrFI RCCGGY 4 cut(s) 352, 838, 871, 880
BssAI RCCGGY 4 cut(s) 352, 838, 871, 880
BssECI CCNNGG 3 cut(s) 33, 270, 293
BssMI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
BssNI GRCGYC 8 cut(s) 21, 78, 108, 126, 335, 565, 705, 836
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 2 cut(s) 199, 205
Bst6I CTCTTC 1 cut(s) 777
BstACI GRCGYC 8 cut(s) 21, 78, 108, 126, 335, 565, 705, 836
BstC8I GCNNGC 6 cut(s) 330, 354, 607, 703, 840, 873
BstDSI CCRYGG 1 cut(s) 293
BstFNI CGCG 3 cut(s) 209, 295, 820
BstH2I RGCGCY 4 cut(s) 338, 365, 568, 708
BstHHI GCGC 6 cut(s) 211, 337, 364, 567, 707, 844
BstKTI GATC 8 cut(s) 283, 417, 505, 510, 643, 660, 774, 888
BstMBI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
BstMCI CGRYCG 3 cut(s) 261, 293, 688
BstNI CCWGG 1 cut(s) 664
BstSCI CCNGG 3 cut(s) 213, 312, 662
BstUI CGCG 3 cut(s) 209, 295, 820
BstV1I GCAGC 6 cut(s) 175, 309, 408, 411, 841, 856
BstV2I GAAGAC 2 cut(s) 81, 732
BstX2I RGATCY 2 cut(s) 280, 657
BstYI RGATCY 2 cut(s) 280, 657
BstZI CGGCCG 3 cut(s) 258, 290, 685
BsuRI GGCC 6 cut(s) 5, 260, 292, 647, 687, 788
BtgI CCRYGG 1 cut(s) 293
Cac8I GCNNGC 6 cut(s) 330, 354, 607, 703, 840, 873
CaiI CAGNNNCTG 1 cut(s) 394
CciNI GCGGCCGC 1 cut(s) 290
CfoI GCGC 6 cut(s) 211, 337, 364, 567, 707, 844
Cfr10I RCCGGY 4 cut(s) 352, 838, 871, 880
Cfr13I GGNCC 1 cut(s) 4
Cfr42I CCGCGG 1 cut(s) 296
CseI GACGC 3 cut(s) 29, 457, 844
Csp6I GTAC 2 cut(s) 307, 580
CspCI CAANNNNNGTGG 2 cut(s) 901, 936
CviQI GTAC 2 cut(s) 307, 580
DinI GGCGCC 3 cut(s) 336, 566, 706
DpnI GATC 8 cut(s) 282, 416, 504, 509, 642, 659, 773, 887
DpnII GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
DraIII CACNNNGTG 1 cut(s) 907
DrdI GACNNNNNNGTC 2 cut(s) 107, 125
DseDI GACNNNNNNGTC 2 cut(s) 107, 125
EaeI YGGCCR 3 cut(s) 258, 290, 685
EagI CGGCCG 3 cut(s) 258, 290, 685
Eam1104I CTCTTC 1 cut(s) 777
EarI CTCTTC 1 cut(s) 777
EciI GGCGGA 1 cut(s) 127
EclXI CGGCCG 3 cut(s) 258, 290, 685
Eco24I GRGCYC 1 cut(s) 635
Eco52I CGGCCG 3 cut(s) 258, 290, 685
Eco57I CTGAAG 1 cut(s) 590
Eco88I CYCGRG 2 cut(s) 32, 373
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 662
EcoT38I GRGCYC 1 cut(s) 635
EgeI GGCGCC 3 cut(s) 336, 566, 706
EheI GGCGCC 3 cut(s) 336, 566, 706
FaiI YATR 2 cut(s) 179, 673
FauI CCCGC 1 cut(s) 461
FbaI TGATCA 1 cut(s) 885
FblI GTMKAC 2 cut(s) 81, 111
FriOI GRGCYC 1 cut(s) 635
GlaI GCGC 6 cut(s) 210, 336, 363, 566, 706, 843
GsuI CTGGAG 2 cut(s) 506, 646
HaeII RGCGCY 4 cut(s) 338, 365, 568, 708
HaeIII GGCC 6 cut(s) 5, 260, 292, 647, 687, 788
HgaI GACGC 3 cut(s) 29, 457, 844
HhaI GCGC 6 cut(s) 211, 337, 364, 567, 707, 844
Hin1I GRCGYC 8 cut(s) 21, 78, 108, 126, 335, 565, 705, 836
Hin6I GCGC 6 cut(s) 209, 335, 362, 565, 705, 842
HinP1I GCGC 6 cut(s) 209, 335, 362, 565, 705, 842
HincII GTYRAC 3 cut(s) 82, 88, 112
HindII GTYRAC 3 cut(s) 82, 88, 112
HinfI GANTC 3 cut(s) 301, 371, 895
HphI GGTGA 2 cut(s) 193, 895
Hpy166II GTNNAC 6 cut(s) 82, 88, 112, 399, 807, 910
Hpy188I TCNGA 5 cut(s) 271, 285, 507, 695, 801
Hpy188III TCNNGA 6 cut(s) 437, 628, 638, 655, 769, 775
Hpy8I GTNNAC 6 cut(s) 82, 88, 112, 399, 807, 910
HpyAV CCTTC 2 cut(s) 156, 661
HpyCH4III ACNGT 2 cut(s) 199, 205
HpyCH4IV ACGT 4 cut(s) 78, 84, 108, 126
HpyCH4V TGCA 1 cut(s) 427
HpySE526I ACGT 4 cut(s) 78, 84, 108, 126
Hsp92I GRCGYC 8 cut(s) 21, 78, 108, 126, 335, 565, 705, 836
HspAI GCGC 6 cut(s) 209, 335, 362, 565, 705, 842
KasI GGCGCC 3 cut(s) 334, 564, 704
KroI GCCGGC 3 cut(s) 352, 838, 871
KroNI GCCGGC 3 cut(s) 354, 840, 873
Ksp22I TGATCA 1 cut(s) 885
KspI CCGCGG 1 cut(s) 296
Kzo9I GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
LmnI GCTCC 7 cut(s) 196, 216, 223, 241, 525, 564, 675
Lsp1109I GCAGC 6 cut(s) 175, 309, 408, 411, 841, 856
MaeII ACGT 4 cut(s) 78, 84, 108, 126
MaeIII GTNAC 1 cut(s) 199
MalI GATC 8 cut(s) 282, 416, 504, 509, 642, 659, 773, 887
MbiI CCGCTC 1 cut(s) 236
MboI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
MboII GAAGA 8 cut(s) 83, 86, 358, 583, 586, 679, 737, 794
MflI RGATCY 2 cut(s) 280, 657
MhlI GDGCHC 1 cut(s) 635
MluCI AATT 6 cut(s) 133, 160, 530, 754, 960, 969
Mly113I GGCGCC 3 cut(s) 335, 565, 705
MlyI GAGTC 1 cut(s) 365
MreI CGCCGGCG 1 cut(s) 838
MroNI GCCGGC 3 cut(s) 352, 838, 871
MslI CAYNNNNRTG 1 cut(s) 903
MspA1I CMGCKG 8 cut(s) 188, 295, 328, 391, 526, 547, 609, 832
MspR9I CCNGG 3 cut(s) 215, 314, 664
MvaI CCWGG 1 cut(s) 664
MvnI CGCG 3 cut(s) 209, 295, 820
NaeI GCCGGC 3 cut(s) 354, 840, 873
NarI GGCGCC 3 cut(s) 335, 565, 705
NciI CCSGG 2 cut(s) 215, 314
NdeII GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
NgoMIV GCCGGC 3 cut(s) 352, 838, 871
NlaIV GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 879
NmeAIII GCCGAG 1 cut(s) 882
NmuCI GTSAC 1 cut(s) 199
NotI GCGGCCGC 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 373
PcsI WCGNNNNNNNCGW 9 cut(s) 87, 102, 108, 111, 114, 117, 123, 251, 717
PdiI GCCGGC 3 cut(s) 354, 840, 873
PfeI GAWTC 2 cut(s) 301, 895
PflFI GACNNNGTC 2 cut(s) 107, 314
PflMI CCANNNNNTGG 1 cut(s) 550
PfoI TCCNGGA 1 cut(s) 662
PleI GAGTC 1 cut(s) 365
PluTI GGCGCC 3 cut(s) 338, 568, 708
PpsI GAGTC 1 cut(s) 365
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 7 cut(s) 6, 219, 282, 336, 566, 706, 879
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 2 cut(s) 280, 657
PsyI GACNNNGTC 2 cut(s) 107, 314
PvuII CAGCTG 2 cut(s) 526, 547
RsaI GTAC 2 cut(s) 308, 581
RsaNI GTAC 2 cut(s) 307, 580
RseI CAYNNNNRTG 1 cut(s) 903
SacII CCGCGG 1 cut(s) 296
SalI GTCGAC 2 cut(s) 80, 110
Sau3AI GATC 8 cut(s) 280, 414, 502, 507, 640, 657, 771, 885
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 3 cut(s) 215, 314, 664
SduI GDGCHC 1 cut(s) 635
SfoI GGCGCC 3 cut(s) 336, 566, 706
Sfr274I CTCGAG 1 cut(s) 373
Sfr303I CCGCGG 1 cut(s) 296
SgrAI CRCCGGYG 1 cut(s) 838
SgrBI CCGCGG 1 cut(s) 296
SgrDI CGTCGACG 2 cut(s) 80, 110
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 903
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 6 cut(s) 133, 160, 530, 754, 960, 969
SspDI GGCGCC 3 cut(s) 334, 564, 704
StyD4I CCNGG 3 cut(s) 213, 312, 662
TaaI ACNGT 2 cut(s) 199, 205
TaiI ACGT 4 cut(s) 81, 87, 111, 129
TaqI TCGA 8 cut(s) 81, 111, 374, 417, 515, 654, 768, 867
TasI AATT 6 cut(s) 133, 160, 530, 754, 960, 969
TfiI GAWTC 2 cut(s) 301, 895
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 6 cut(s) 188, 322, 421, 424, 829, 844
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 2 cut(s) 416, 794
Tth111I GACNNNGTC 2 cut(s) 107, 314
Van91I CCANNNNNTGG 1 cut(s) 550
XapI RAATTY 1 cut(s) 530
XhoI CTCGAG 1 cut(s) 373
XmiI GTMKAC 2 cut(s) 81, 111
ZraI GACGTC 3 cut(s) 79, 109, 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.