RchiOBHm_Chr3g0466361

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0011735
Physical Location & Seq
Forward (+)
12834410 .. 12837259
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 909 bp
ATGGAGTTCCATGAAGACCGTTACAGGCAGGCTCAGAGGCCAAAATATGATTGTCTTCTATTTGATCTAGATGATACCCTTTATCCCCTAAGTACTGGTGTTGCTACTGCTTGCCGCAATAACATAGAAGATTATATGGTTGAAAAGCTTGGCATTGAGAGGAGCATCATCCCTGAATTGGGTAACCTGCTGTACAAGAATTATGGAACTACAATGGCTGGACTTAGGGCAATTGGCTATGACTTTGACTATGATGAATACCACAGTTTTGTCCATGGTAGATTACCTTATGAGAACATAAAACCAGACCCTGTTTTGAGGAGTCTTTTGCTCAACCTGCCTTACAGGAAAATTATATTCACAAATGCAGACAAGGTCCATGCTGCTAAAGCACTAAGCAGGCTTGGTTTAGAAGATTGCTTTGAGGGAATTATCTGCTTTGAGACCCTCAACCCTATTCACAAAAGCACTGTTTCTGATGATGAAGATGACATTGAGTTTGTGGGATTAAGCACTGCTCAAATTCCTGCCACAACAACTACTAGTAGCTCTGAGATTTTTGACATCATTGCCCATTTTGCTCAAGCAAACCCCTCTTCAAAATTACCCAAAACCCCAATTGTGTGCAAGCCATCAGAAGCTGCCATAGAAAAAGCCCTCGAGATTGCCCACATTAACCCTCAGAGAACACTGTTCTTTGAAGATAGTGTCAGGAACATACAAGCTGGCAAAAGAGTGGGTCTTCACACTGTGCTGGTTGGCACATCACAGAGAGTTAAAGGTGCTGACTTTGCACTAGAAAGCATCCACAATCTGAGGGAAGCACTACCAGAGCTTTGGGAAGCTGATAGGAAATCTGAAGTTGGTTACCCTGGAAAAGTAGCAGTGGAAACTTCAGTCACAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 map00760
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

302

Amino Acids

33.82

Weight (kDa)

5.25

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_2 PF13419 183 - 253 1.4e-06 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011735)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02230 AT5G02230 AT5G02230
fragaria_vesca FvH4_6g13490 FvH4_6g13490
malus_domestica MD04G1130800.v1.1 MD12G1143900.v1.1
prunus_persica Prupe.6G257300_v2.0.a1 Prupe.6G257300_v2.0.a1 Prupe.6G257300_v2.0.a1
pyrus_communis pycom12g13850
rosa_chinensis RchiOBHm_Chr3g0466361
rosa_laevigata RLG00000024581
rosa_multiflora Rmu_sc0003480.1_g000006
rosa_roxburghii Rroxscaffold_6G00414540
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127700 Rh3DG149700
rosa_wichuraiana Rw3G012060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 195, 345
AciI CCGC 1 cut(s) 115
AcsI RAATTY 1 cut(s) 522
AcuI CTGAAG 2 cut(s) 879, 879
AdeI CACNNNGTG 1 cut(s) 751
AfaI GTAC 2 cut(s) 94, 194
AfiI CCNNNNNNNGG 2 cut(s) 178, 179
AgsI TTSAA 3 cut(s) 143, 600, 701
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 1 cut(s) 871
AluBI AGCT 7 cut(s) 148, 549, 641, 725, 835, 845, 905
AluI AGCT 7 cut(s) 148, 549, 641, 725, 835, 845, 905
Alw26I GTCTC 1 cut(s) 437
AlwNI CAGNNNCTG 2 cut(s) 311, 641
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 1 cut(s) 38
ApeKI GCWGC 2 cut(s) 383, 641
ApoI RAATTY 1 cut(s) 522
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AspS9I GGNCC 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 659
AvaII GGWCC 1 cut(s) 376
BbsI GAAGAC 3 cut(s) 21, 47, 734
BbvI GCAGC 2 cut(s) 370, 628
BccI CCATC 1 cut(s) 640
BciT130I CCWGG 1 cut(s) 873
BcoDI GTCTC 1 cut(s) 437
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 3 cut(s) 68, 543, 797
BfuAI ACCTGC 2 cut(s) 195, 345
BisI GCNGC 3 cut(s) 115, 384, 642
BlsI GCNGC 3 cut(s) 116, 385, 643
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 1 cut(s) 873
Bme18I GGWCC 1 cut(s) 376
BmeT110I CYCGRG 1 cut(s) 659
BmgT120I GGNCC 1 cut(s) 376
BmrFI CCNGG 1 cut(s) 873
BmsI GCATC 2 cut(s) 174, 813
BpiI GAAGAC 3 cut(s) 21, 47, 734
BpuEI CTTGAG 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 274, 871
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 179
Bse1I ACTGG 1 cut(s) 100
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 1 cut(s) 873
BseDI CCNNGG 2 cut(s) 274, 871
BseGI GGATG 2 cut(s) 168, 804
BseLI CCNNNNNNNGG 2 cut(s) 178, 179
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 4 cut(s) 47, 543, 695, 806
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 2 cut(s) 175, 334
BseXI GCAGC 2 cut(s) 370, 628
BshFI GGCC 1 cut(s) 40
BsiHKCI CYCGRG 1 cut(s) 659
BslI CCNNNNNNNGG 2 cut(s) 178, 179
BsmAI GTCTC 1 cut(s) 437
BsnI GGCC 1 cut(s) 40
Bso31I GGTCTC 1 cut(s) 437
BsoBI CYCGRG 1 cut(s) 659
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 1 cut(s) 64
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 1 cut(s) 115
BspANI GGCC 1 cut(s) 40
BspCNI CTCAG 4 cut(s) 46, 544, 694, 807
BspMI ACCTGC 2 cut(s) 195, 345
BspTNI GGTCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 567
BsrGI TGTACA 1 cut(s) 192
BsrI ACTGG 1 cut(s) 100
BssECI CCNNGG 2 cut(s) 274, 871
BssMI GATC 1 cut(s) 64
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 873
Bst4CI ACNGT 5 cut(s) 20, 266, 472, 693, 751
Bst6I CTCTTC 1 cut(s) 601
BstAUI TGTACA 1 cut(s) 192
BstC8I GCNNGC 5 cut(s) 30, 112, 401, 629, 727
BstDEI CTNAG 8 cut(s) 33, 89, 224, 395, 552, 681, 815, 906
BstDSI CCRYGG 1 cut(s) 274
BstEII GGTNACC 2 cut(s) 182, 866
BstF5I GGATG 2 cut(s) 168, 804
BstKTI GATC 1 cut(s) 67
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 1 cut(s) 64
BstMWI GCNNNNNNNGC 4 cut(s) 337, 389, 578, 791
BstNI CCWGG 1 cut(s) 873
BstPI GGTNACC 2 cut(s) 182, 866
BstSCI CCNGG 1 cut(s) 871
BstV1I GCAGC 2 cut(s) 370, 628
BstV2I GAAGAC 3 cut(s) 21, 47, 734
BstXI CCANNNNNNTGG 1 cut(s) 837
BsuRI GGCC 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 274
BtsCI GGATG 2 cut(s) 168, 804
BtsI GCAGTG 2 cut(s) 513, 891
BtsIMutI CAGTG 5 cut(s) 468, 513, 689, 747, 891
BveI ACCTGC 2 cut(s) 195, 345
Cac8I GCNNGC 5 cut(s) 30, 112, 401, 629, 727
CaiI CAGNNNCTG 2 cut(s) 311, 641
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 2 cut(s) 93, 193
CviAII CATG 3 cut(s) 11, 275, 380
CviQI GTAC 2 cut(s) 93, 193
DdeI CTNAG 8 cut(s) 33, 89, 224, 395, 552, 681, 815, 906
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
DraIII CACNNNGTG 1 cut(s) 751
Eam1104I CTCTTC 1 cut(s) 601
EarI CTCTTC 1 cut(s) 601
Eco130I CCWWGG 1 cut(s) 274
Eco31I GGTCTC 1 cut(s) 437
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 2 cut(s) 879, 879
Eco88I CYCGRG 1 cut(s) 659
Eco91I GGTNACC 2 cut(s) 182, 866
EcoO65I GGTNACC 2 cut(s) 182, 866
EcoRII CCWGG 1 cut(s) 871
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 3 cut(s) 14, 278, 383
FatI CATG 3 cut(s) 10, 274, 379
Fnu4HI GCNGC 3 cut(s) 115, 384, 642
FokI GGATG 2 cut(s) 155, 791
Fsp4HI GCNGC 3 cut(s) 115, 384, 642
FspBI CTAG 3 cut(s) 68, 543, 797
GluI GCNGC 3 cut(s) 115, 384, 642
HaeIII GGCC 1 cut(s) 40
Hin1II CATG 3 cut(s) 14, 278, 383
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 1 cut(s) 322
Hpy188I TCNGA 7 cut(s) 36, 478, 553, 637, 684, 816, 859
Hpy188III TCNNGA 3 cut(s) 68, 661, 712
HpyCH4III ACNGT 5 cut(s) 20, 266, 472, 693, 751
HpyCH4V TGCA 3 cut(s) 368, 627, 794
HpyF10VI GCNNNNNNNGC 4 cut(s) 337, 389, 578, 791
HpyF3I CTNAG 8 cut(s) 33, 89, 224, 395, 552, 681, 815, 906
Hsp92II CATG 3 cut(s) 14, 278, 383
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 1 cut(s) 162
Lsp1109I GCAGC 2 cut(s) 370, 628
LweI GCATC 2 cut(s) 174, 813
MaeI CTAG 3 cut(s) 68, 543, 797
MaeIII GTNAC 4 cut(s) 20, 182, 866, 898
MalI GATC 1 cut(s) 66
MboI GATC 1 cut(s) 64
MboII GAAGA 8 cut(s) 26, 47, 140, 425, 497, 588, 713, 734
MfeI CAATTG 2 cut(s) 231, 618
MluCI AATT 8 cut(s) 176, 199, 231, 351, 429, 522, 602, 618
MlyI GAGTC 1 cut(s) 331
MnlI CCTC 9 cut(s) 30, 153, 312, 418, 458, 604, 668, 690, 810
MseI TTAA 3 cut(s) 509, 675, 777
MspR9I CCNGG 1 cut(s) 873
MunI CAATTG 2 cut(s) 231, 618
MvaI CCWGG 1 cut(s) 873
MwoI GCNNNNNNNGC 4 cut(s) 337, 389, 578, 791
NcoI CCATGG 1 cut(s) 274
NdeII GATC 1 cut(s) 64
NlaIII CATG 3 cut(s) 14, 278, 383
NmuCI GTSAC 1 cut(s) 898
PaeR7I CTCGAG 1 cut(s) 659
PflFI GACNNNGTC 1 cut(s) 374
PkrI GCNGC 3 cut(s) 116, 385, 643
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 871
PspEI GGTNACC 2 cut(s) 182, 866
PspGI CCWGG 1 cut(s) 871
PspPI GGNCC 1 cut(s) 376
PstNI CAGNNNCTG 2 cut(s) 311, 641
PsyI GACNNNGTC 1 cut(s) 374
RsaI GTAC 2 cut(s) 94, 194
RsaNI GTAC 2 cut(s) 93, 193
SaqAI TTAA 3 cut(s) 509, 675, 777
SatI GCNGC 3 cut(s) 115, 384, 642
Sau3AI GATC 1 cut(s) 64
Sau96I GGNCC 1 cut(s) 376
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 1 cut(s) 873
SfaNI GCATC 2 cut(s) 174, 813
Sfr274I CTCGAG 1 cut(s) 659
SinI GGWCC 1 cut(s) 376
SlaI CTCGAG 1 cut(s) 659
SmlI CTYRAG 2 cut(s) 582, 659
SmoI CTYRAG 2 cut(s) 582, 659
SpeI ACTAGT 1 cut(s) 542
Sse9I AATT 8 cut(s) 176, 199, 231, 351, 429, 522, 602, 618
SsiI CCGC 1 cut(s) 115
SspMI CTAG 3 cut(s) 68, 543, 797
StyD4I CCNGG 1 cut(s) 871
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 5 cut(s) 20, 266, 472, 693, 751
TaqI TCGA 1 cut(s) 660
TasI AATT 8 cut(s) 176, 199, 231, 351, 429, 522, 602, 618
TatI WGTACW 2 cut(s) 92, 192
TauI GCSGC 1 cut(s) 117
Tru1I TTAA 3 cut(s) 509, 675, 777
Tru9I TTAA 3 cut(s) 509, 675, 777
TscAI CASTG 5 cut(s) 475, 520, 696, 754, 891
TseFI GTSAC 1 cut(s) 898
TseI GCWGC 2 cut(s) 383, 641
Tsp45I GTSAC 1 cut(s) 898
TspDTI ATGAA 3 cut(s) 27, 270, 498
TspRI CASTG 5 cut(s) 475, 520, 696, 754, 891
Tth111I GACNNNGTC 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 1 cut(s) 522
XbaI TCTAGA 1 cut(s) 67
XhoI CTCGAG 1 cut(s) 659
XspI CTAG 3 cut(s) 68, 543, 797
ZrmI AGTACT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.