RchiOBHm_Chr3g0472961

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0013245
Physical Location & Seq
Forward (+)
18912712 .. 18915708
2997 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ATGGGTGCGCAGAGGCTGGGACTGTTTCTCGTTCTGACATTATTGGGGTTGGCCTTGCCGGAGTTCTCTGGTGTTGCAGAGGGGTATAACCGCCCACCGGCCCGGAAGAATCTCGACGTGCCGTCGGATACAGATCCTGACTCCAATTCTCCAGAACAGGTGCATATATCTCTGGTTGGTGAAGACAAAATGAGAGTATCATGGACGACAAAGAACCCAAGTCCAGCAACAGTGGAATACGGCACATCTCCTGGTGCTTATTCAAGCTCTGCAACTGGAAGTACAACTTCATACTCCTATTTATTGTACCACAGTGGAGAATTCCATCATGTTGACATCGGTCCATTGAATGCCAACACAGTATACTACTACCGCTGCGGCTCCAGTCCGGGTCCTGAGTTCAGTTTCAAAACCCCTCCTGCTGCCTTCCCTCTCACATTTGCAGTGGCAGGTGATCTTGGACAGACTGAGTGGACTAACGAAACCCTCCAACACATAAGCAAATCACCATATGACGTACTCCTATTGCCCGGAGACTTGTCCTATGCTGATTTCAAGCAAAATTTATGGGACTCATTCGGCCGATTGGTCCAGCCACTGGCAAGCCAGCGGCCGTGGATGGTCACAGAAGGCAACCATGAGGTCGAGAAGATCCCAGTGGTGCATTCTGAACCCTTCACGGCATACAACGCCAGGTGGAGAATGCCGTTCGAACAGAGTGGGTCGGAATCCAACCTCTACTATTCCTTCAATGTGGCAGGAGTCCATGTTATCATGTTGGGTTCATACACCGATTTTGATCCTGCTTCTTTACAATACAAGTGGCTACAGACGGATTTGGCAAAGGTTGACAGGGGGAGAACTCCTTGGGTTGTGGTGCTTATCCATGCCCCATGGTACAACTCTAACGAAGCTCATCAGGGTGAATCCGAATCAATTGACATGAAGAGTTCCATGGAAGAATTACTCTATAAAGGTCGTGTTGATCTTGTTTTTGCAGGACATGTACATGCCTATGAGCGTTTCACTCGAGTTTACAAAGACAAAGCAGATGATTGTGGTCCGGTGCATATTACTATTGGAGATGGAGGCAACCGCGAAGGACTTGCCACAAAGTATGAAGAACCAAAACCGGCAATATCAATGTTCAGGGAGGCGAGCTTTGGGCATGGACAGTTGCAGGTAGTGAATGGAACCCATGCGCTGTGGACATGGCGTACGAATCAGGATGATAATGAGACTGTTGCAAGTGATAGCATTTGGCTCACAAGCCTCTTGGCTACTCCTGCTTGTAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

433

Amino Acids

48.15

Weight (kDa)

5.36

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 51 - 138 2.5e-22 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 146 - 339 7.9e-20 Calcineurin-like phosphoesterase
Metallophos_C PF14008 354 - 414 1.5e-19 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52780 AT3G52780
fragaria_vesca FvH4_6g18430 FvH4_6g18430
malus_domestica MD12G1023400.v1.1 MD14G1020400.v1.1
prunus_persica Prupe.7G113100_v2.0.a1
pyrus_communis pycom12g02180
rosa_chinensis RchiOBHm_Chr3g0472961
rosa_laevigata RLG00000024044
rosa_multiflora Rmu_sc0021557.1_g000005
rosa_roxburghii Rroxscaffold_6G00408460
rosa_rugosa Rorug03G0129900
rosa_samantha Rh3AG182000 Rh3BG208300 Rh3CG205300 Rh3DG167800
rosa_wichuraiana Rw3G016150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 440
Acc16I TGCGCA 1 cut(s) 9
Acc36I ACCTGC 2 cut(s) 440, 1171
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 1 cut(s) 363
AccII CGCG 1 cut(s) 1098
AciI CCGC 5 cut(s) 91, 373, 378, 610, 1096
AclWI GGATC 3 cut(s) 128, 646, 794
AcoI YGGCCR 2 cut(s) 580, 611
AcsI RAATTY 2 cut(s) 320, 562
AfaI GTAC 6 cut(s) 283, 308, 519, 899, 1008, 1219
AfiI CCNNNNNNNGG 2 cut(s) 97, 598
AflIII ACRYGT 1 cut(s) 1003
AgsI TTSAA 5 cut(s) 264, 349, 409, 556, 751
AhdI GACNNNNNGTC 1 cut(s) 121
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 2 cut(s) 250, 692
AluBI AGCT 3 cut(s) 267, 914, 1161
AluI AGCT 3 cut(s) 267, 914, 1161
Alw26I GTCTC 2 cut(s) 528, 1232
AlwI GGATC 3 cut(s) 128, 646, 794
AlwNI CAGNNNCTG 3 cut(s) 16, 137, 598
Ama87I CYCGRG 1 cut(s) 1029
AoxI GGCC 4 cut(s) 51, 99, 580, 611
ApeKI GCWGC 2 cut(s) 375, 422
ApoI RAATTY 2 cut(s) 320, 562
AspLEI GCGC 2 cut(s) 10, 1204
AspS9I GGNCC 5 cut(s) 100, 341, 392, 589, 1061
AsuC2I CCSGG 3 cut(s) 103, 390, 531
AsuHPI GGTGA 4 cut(s) 191, 464, 498, 935
AsuII TTCGAA 1 cut(s) 711
AvaI CYCGRG 1 cut(s) 1029
AvaII GGWCC 4 cut(s) 341, 392, 589, 1061
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 362, 409
BccI CCATC 3 cut(s) 333, 613, 1079
BceAI ACGGC 5 cut(s) 106, 256, 598, 691, 696
BcgI CGANNNNNNTGC 2 cut(s) 1088, 1122
BciT130I CCWGG 2 cut(s) 252, 694
BciVI GTATCC 1 cut(s) 121
BcnI CCSGG 3 cut(s) 103, 390, 531
BcoDI GTCTC 2 cut(s) 528, 1232
BfmI CTRYAG 1 cut(s) 827
BfuAI ACCTGC 2 cut(s) 440, 1171
BfuI GTATCC 1 cut(s) 121
BisI GCNGC 4 cut(s) 376, 379, 423, 611
BlsI GCNGC 4 cut(s) 377, 380, 424, 612
Bme1390I CCNGG 5 cut(s) 103, 252, 390, 531, 694
Bme18I GGWCC 4 cut(s) 341, 392, 589, 1061
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 1029
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 5 cut(s) 100, 341, 392, 589, 1061
BmiI GGNNCC 3 cut(s) 382, 393, 1195
BmrFI CCNGG 5 cut(s) 103, 252, 390, 531, 694
BmrI ACTGGG 1 cut(s) 650
BmuI ACTGGG 1 cut(s) 650
BoxI GACNNNNGTC 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 189
BpmI CTGGAG 2 cut(s) 135, 367
Bpu14I TTCGAA 1 cut(s) 711
BpuMI CCSGG 3 cut(s) 103, 390, 531
BsaBI GATNNNNATC 2 cut(s) 132, 798
BsaJI CCNNGG 4 cut(s) 614, 866, 893, 954
BsaWI WCCGGW 1 cut(s) 1063
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 598
Bse118I RCCGGY 2 cut(s) 97, 1132
Bse1I ACTGG 4 cut(s) 280, 384, 603, 656
Bse8I GATNNNNATC 2 cut(s) 132, 798
BseBI CCWGG 2 cut(s) 252, 694
BseDI CCNNGG 4 cut(s) 614, 866, 893, 954
BseGI GGATG 2 cut(s) 624, 1234
BseJI GATNNNNATC 2 cut(s) 132, 798
BseLI CCNNNNNNNGG 2 cut(s) 97, 598
BseMII CTCAG 2 cut(s) 387, 459
BseNI ACTGG 4 cut(s) 280, 384, 603, 656
BseX3I CGGCCG 2 cut(s) 580, 611
BseXI GCAGC 2 cut(s) 362, 409
BseYI CCCAGC 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 1098
Bsh1285I CGRYCG 2 cut(s) 583, 614
BshFI GGCC 4 cut(s) 53, 101, 582, 613
BsiEI CGRYCG 2 cut(s) 583, 614
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 7 cut(s) 59, 98, 103, 389, 531, 1064, 1133
BsiWI CGTACG 1 cut(s) 1217
BslFI GGGAC 2 cut(s) 33, 584
BslI CCNNNNNNNGG 2 cut(s) 97, 598
BsmAI GTCTC 2 cut(s) 528, 1232
BsmFI GGGAC 2 cut(s) 33, 584
BsmI GAATGC 3 cut(s) 355, 664, 708
BsnI GGCC 4 cut(s) 53, 101, 582, 613
BsoBI CYCGRG 1 cut(s) 1029
Bsp119I TTCGAA 1 cut(s) 711
Bsp1407I TGTACA 1 cut(s) 1006
Bsp143I GATC 5 cut(s) 133, 454, 651, 799, 985
Bsp19I CCATGG 2 cut(s) 893, 954
BspACI CCGC 5 cut(s) 91, 373, 378, 610, 1096
BspANI GGCC 4 cut(s) 53, 101, 582, 613
BspCNI CTCAG 2 cut(s) 388, 460
BspFNI CGCG 1 cut(s) 1098
BspLI GGNNCC 3 cut(s) 382, 393, 1195
BspMI ACCTGC 2 cut(s) 440, 1171
BspPI GGATC 3 cut(s) 128, 646, 794
BspT104I TTCGAA 1 cut(s) 711
BsrFI RCCGGY 2 cut(s) 97, 1132
BsrGI TGTACA 1 cut(s) 1006
BsrI ACTGG 4 cut(s) 280, 384, 603, 656
BssAI RCCGGY 2 cut(s) 97, 1132
BssECI CCNNGG 4 cut(s) 614, 866, 893, 954
BssMI GATC 5 cut(s) 133, 454, 651, 799, 985
BssNAI GTATAC 1 cut(s) 364
BssT1I CCWWGG 3 cut(s) 866, 893, 954
Bst1107I GTATAC 1 cut(s) 364
Bst2UI CCWGG 2 cut(s) 252, 694
Bst4CI ACNGT 6 cut(s) 24, 232, 314, 361, 1176, 1243
Bst6I CTCTTC 1 cut(s) 941
BstAUI TGTACA 1 cut(s) 1006
BstBI TTCGAA 1 cut(s) 711
BstC8I GCNNGC 3 cut(s) 604, 608, 1159
BstDEI CTNAG 2 cut(s) 396, 468
BstDSI CCRYGG 3 cut(s) 614, 893, 954
BstF5I GGATG 2 cut(s) 624, 1234
BstFNI CGCG 1 cut(s) 1098
BstHHI GCGC 2 cut(s) 10, 1204
BstKTI GATC 5 cut(s) 136, 457, 654, 802, 988
BstMAI GTCTC 2 cut(s) 528, 1232
BstMBI GATC 5 cut(s) 133, 454, 651, 799, 985
BstMCI CGRYCG 2 cut(s) 583, 614
BstMWI GCNNNNNNNGC 2 cut(s) 689, 1286
BstNI CCWGG 2 cut(s) 252, 694
BstNSI RCATGY 2 cut(s) 1007, 1013
BstPAI GACNNNNGTC 1 cut(s) 339
BstSCI CCNGG 5 cut(s) 101, 250, 388, 529, 692
BstSFI CTRYAG 1 cut(s) 827
BstUI CGCG 1 cut(s) 1098
BstV1I GCAGC 2 cut(s) 362, 409
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 133, 651
BstYI RGATCY 2 cut(s) 133, 651
BstZ17I GTATAC 1 cut(s) 364
BstZI CGGCCG 2 cut(s) 580, 611
BsuI GTATCC 1 cut(s) 121
BsuRI GGCC 4 cut(s) 53, 101, 582, 613
BtgI CCRYGG 3 cut(s) 614, 893, 954
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 2 cut(s) 624, 1234
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 5 cut(s) 237, 319, 450, 596, 663
BveI ACCTGC 2 cut(s) 440, 1171
Cac8I GCNNGC 3 cut(s) 604, 608, 1159
CaiI CAGNNNCTG 3 cut(s) 16, 137, 598
CfoI GCGC 2 cut(s) 10, 1204
Cfr10I RCCGGY 2 cut(s) 97, 1132
Cfr13I GGNCC 5 cut(s) 100, 341, 392, 589, 1061
Csp6I GTAC 6 cut(s) 282, 307, 518, 898, 1007, 1218
CspCI CAANNNNNGTGG 2 cut(s) 803, 838
CviQI GTAC 6 cut(s) 282, 307, 518, 898, 1007, 1218
DdeI CTNAG 2 cut(s) 396, 468
DpnI GATC 5 cut(s) 135, 456, 653, 801, 987
DpnII GATC 5 cut(s) 133, 454, 651, 799, 985
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 2 cut(s) 580, 611
EagI CGGCCG 2 cut(s) 580, 611
Eam1104I CTCTTC 1 cut(s) 941
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 941
EclXI CGGCCG 2 cut(s) 580, 611
Eco130I CCWWGG 3 cut(s) 866, 893, 954
Eco47I GGWCC 4 cut(s) 341, 392, 589, 1061
Eco52I CGGCCG 2 cut(s) 580, 611
Eco88I CYCGRG 1 cut(s) 1029
EcoO109I RGGNCCY 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 2 cut(s) 250, 692
EcoT14I CCWWGG 3 cut(s) 866, 893, 954
ErhI CCWWGG 3 cut(s) 866, 893, 954
FalI AAGNNNNNCTT 2 cut(s) 271, 303
FaqI GGGAC 2 cut(s) 33, 584
FauNDI CATATG 1 cut(s) 511
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 4 cut(s) 376, 379, 423, 611
FokI GGATG 2 cut(s) 631, 1241
Fsp4HI GCNGC 4 cut(s) 376, 379, 423, 611
FspI TGCGCA 1 cut(s) 9
GlaI GCGC 2 cut(s) 9, 1203
GluI GCNGC 4 cut(s) 376, 379, 423, 611
GsaI CCCAGC 1 cut(s) 20
GsuI CTGGAG 2 cut(s) 135, 367
HaeIII GGCC 4 cut(s) 53, 101, 582, 613
HapII CCGG 7 cut(s) 59, 98, 103, 389, 531, 1064, 1133
HhaI GCGC 2 cut(s) 10, 1204
Hin6I GCGC 2 cut(s) 8, 1202
HinP1I GCGC 2 cut(s) 8, 1202
HincII GTYRAC 2 cut(s) 334, 850
HindII GTYRAC 2 cut(s) 334, 850
HinfI GANTC 8 cut(s) 109, 140, 572, 728, 762, 926, 932, 1222
HpaII CCGG 7 cut(s) 59, 98, 103, 389, 531, 1064, 1133
HphI GGTGA 4 cut(s) 191, 464, 498, 935
Hpy166II GTNNAC 6 cut(s) 334, 364, 474, 850, 1036, 1209
Hpy188I TCNGA 5 cut(s) 36, 127, 670, 727, 931
Hpy188III TCNNGA 6 cut(s) 113, 137, 152, 395, 646, 1226
Hpy8I GTNNAC 6 cut(s) 334, 364, 474, 850, 1036, 1209
Hpy99I CGWCG 2 cut(s) 119, 127
HpyAV CCTTC 5 cut(s) 436, 623, 685, 757, 1094
HpyCH4III ACNGT 6 cut(s) 24, 232, 314, 361, 1176, 1243
HpyCH4IV ACGT 2 cut(s) 117, 516
HpyCH4V TGCA 9 cut(s) 77, 163, 272, 443, 664, 998, 1069, 1180, 1247
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 1286
HpyF3I CTNAG 2 cut(s) 396, 468
HpySE526I ACGT 2 cut(s) 117, 516
HspAI GCGC 2 cut(s) 8, 1202
Kzo9I GATC 5 cut(s) 133, 454, 651, 799, 985
LmnI GCTCC 1 cut(s) 386
Lsp1109I GCAGC 2 cut(s) 362, 409
MaeII ACGT 2 cut(s) 117, 516
MaeIII GTNAC 1 cut(s) 622
MalI GATC 5 cut(s) 135, 456, 653, 801, 987
MboI GATC 5 cut(s) 133, 454, 651, 799, 985
MboII GAAGA 6 cut(s) 118, 194, 661, 958, 971, 1133
MfeI CAATTG 1 cut(s) 936
MflI RGATCY 2 cut(s) 133, 651
MluCI AATT 5 cut(s) 145, 320, 562, 936, 962
MlyI GAGTC 3 cut(s) 134, 566, 771
MmeI TCCRAC 4 cut(s) 105, 514, 705, 756
MslI CAYNNNNRTG 3 cut(s) 921, 1008, 1014
MspA1I CMGCKG 2 cut(s) 375, 610
MspI CCGG 7 cut(s) 59, 98, 103, 389, 531, 1064, 1133
MspR9I CCNGG 5 cut(s) 103, 252, 390, 531, 694
MunI CAATTG 1 cut(s) 936
Mva1269I GAATGC 3 cut(s) 355, 664, 708
MvaI CCWGG 2 cut(s) 252, 694
MvnI CGCG 1 cut(s) 1098
MwoI GCNNNNNNNGC 2 cut(s) 689, 1286
NciI CCSGG 3 cut(s) 103, 390, 531
NcoI CCATGG 2 cut(s) 893, 954
NdeI CATATG 1 cut(s) 511
NdeII GATC 5 cut(s) 133, 454, 651, 799, 985
NlaIV GGNNCC 3 cut(s) 382, 393, 1195
NmuCI GTSAC 1 cut(s) 622
NsbI TGCGCA 1 cut(s) 9
NspI RCATGY 2 cut(s) 1007, 1013
NspV TTCGAA 1 cut(s) 711
PaeR7I CTCGAG 1 cut(s) 1029
PaqCI CACCTGC 1 cut(s) 440
PciI ACATGT 1 cut(s) 1003
PctI GAATGC 3 cut(s) 355, 664, 708
PfeI GAWTC 5 cut(s) 109, 728, 926, 932, 1222
Pfl23II CGTACG 1 cut(s) 1217
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 4 cut(s) 377, 380, 424, 612
PleI GAGTC 3 cut(s) 134, 566, 770
PpsI GAGTC 3 cut(s) 134, 566, 770
PpuMI RGGWCCY 1 cut(s) 392
PscI ACATGT 1 cut(s) 1003
PshAI GACNNNNGTC 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 250, 692
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 2 cut(s) 250, 692
PspLI CGTACG 1 cut(s) 1217
PspN4I GGNNCC 3 cut(s) 382, 393, 1195
PspPI GGNCC 5 cut(s) 100, 341, 392, 589, 1061
PspPPI RGGWCCY 1 cut(s) 392
PspXI VCTCGAGB 1 cut(s) 1029
PstNI CAGNNNCTG 3 cut(s) 16, 137, 598
PsuI RGATCY 2 cut(s) 133, 651
RsaI GTAC 6 cut(s) 283, 308, 519, 899, 1008, 1219
RsaNI GTAC 6 cut(s) 282, 307, 518, 898, 1007, 1218
RseI CAYNNNNRTG 3 cut(s) 921, 1008, 1014
SatI GCNGC 4 cut(s) 376, 379, 423, 611
Sau3AI GATC 5 cut(s) 133, 454, 651, 799, 985
Sau96I GGNCC 5 cut(s) 100, 341, 392, 589, 1061
SchI GAGTC 3 cut(s) 134, 566, 771
ScrFI CCNGG 5 cut(s) 103, 252, 390, 531, 694
SfcI CTRYAG 1 cut(s) 827
Sfr274I CTCGAG 1 cut(s) 1029
SfuI TTCGAA 1 cut(s) 711
SinI GGWCC 4 cut(s) 341, 392, 589, 1061
SlaI CTCGAG 1 cut(s) 1029
SmiMI CAYNNNNRTG 3 cut(s) 921, 1008, 1014
SmlI CTYRAG 1 cut(s) 1029
SmoI CTYRAG 1 cut(s) 1029
Sse9I AATT 5 cut(s) 145, 320, 562, 936, 962
SsiI CCGC 5 cut(s) 91, 373, 378, 610, 1096
StyD4I CCNGG 5 cut(s) 101, 250, 388, 529, 692
StyI CCWWGG 3 cut(s) 866, 893, 954
TaaI ACNGT 6 cut(s) 24, 232, 314, 361, 1176, 1243
TaiI ACGT 2 cut(s) 120, 519
TaqI TCGA 4 cut(s) 114, 645, 711, 1030
TaqII GACCGA 1 cut(s) 329
TasI AATT 5 cut(s) 145, 320, 562, 936, 962
TatI WGTACW 2 cut(s) 281, 1006
TauI GCSGC 2 cut(s) 381, 613
TfiI GAWTC 5 cut(s) 109, 728, 926, 932, 1222
TscAI CASTG 5 cut(s) 237, 319, 450, 603, 663
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 2 cut(s) 375, 422
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 4 cut(s) 279, 774, 959, 1134
TspGWI ACGGA 1 cut(s) 848
TspRI CASTG 5 cut(s) 237, 319, 450, 603, 663
Van91I CCANNNNNTGG 1 cut(s) 598
VpaK11BI GGWCC 4 cut(s) 341, 392, 589, 1061
XapI RAATTY 2 cut(s) 320, 562
XceI RCATGY 2 cut(s) 1007, 1013
XhoI CTCGAG 1 cut(s) 1029
XmiI GTMKAC 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.