RchiOBHm_Chr2g0091071

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016610
Physical Location & Seq
Forward (+)
4780849 .. 4784374
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1500 bp
ATGTTGGATGAGAGTAAATTTGATGTGCATTTAAAATTGTGGGCGCTTCGGATTCCTCGTGAGCTTTGCAAGGTCGCCACTCGAATACTAAATGGATATTTGCTTGATAAACCACGCATTAAGCCGATTACGGAGGACCCAACATGTGAAAGAAACCGTTACATGATATTATCGGAGAGGGTGCAAGATTCCGACCTATCTATTATTCCGGATACAATTCTCAATGAACTGAAGGATTTATGCAAGATTGAAGTAGTTCCATATTCGTTGACTCTCGGGTACTCATATTGGGGTGCAGACCATATTTTGAAGCAGATTCTGCCCCCCGGAGTGGAGGTTCCTTCATCTTTTGAAACAATAGTTAAGTACTCAGCTCATGATTCTACTTGGAGTCACGTTGCCCATCTGAATATACATGATGAGTTAATTCCCTACAAGGATGTGATTGCCAAAGTTATTTATGATAAGAATTATCCAAGAATCAAGACAATTGTCAATAAAGTTGGATCTATTTCAAATGAGTTTCGCGTGCCAAAGTTTGAAGTTTTAGCAGGAGAAAAAGATATGGTTACAGAAGTGAAACAATATGGAGCGACTTTCAAGCTTGACTACAGCTTGGTCTATTGGAATTCAAGATTGGAACATGAACATATAAGGTTGGTTTCTCAGTTTCGAGCTGGGGAAGTCATATGTGATATGTTTGCTGGTATTGGCCCTTTTGCTATTCCTGCCGCACAGAAAGGATGCATCGTGTATGCAAATGACTTAAATCCGGATAGCATTCATTATCTGAAGATCAATGCAGAGATTAACAAGGTTGGTGACCATGTTCGCACATACAATCTTGATGCGAGAGAATTTGTTTCTCAATTGATGGCAGTGCCTGACTGTGGGAAAGAATTGAATTCAGATGCGTCCATGCTCAAATCTTGCGAGACATGTACCAATCAGGGTAGTCAGGAATCAGAATCAGGCAATGGAATGCTAAACGTTGAGGCAAAAGAGGTACTAGATAGTGTTACTTCTAATGTGGACGGTCTACAAAGTTCTAGTAGGAATACAGATGCTTCAGTACCTCCAGTTAAAAGACCTGCAGATATTTGTCAATCAGAGAATGGAATTGTTAATGGCACAAACGTTCTTGTAGCTGGTAGGAGCAAAGGAAACAAGAATAAGAGATTAAGAGGCTCTGAGATGATCAATGTCAAGACTTGGGAGCATGTTGATCATGTGATAATGAACCTTCCGGCTTCTGCTCTTGAATTTCTAGATGCATTTAGAGGGGTGATCCAGAGAAAATATTGGAGGGAAACTCTTCCTTTGATTCACTGCTATTGCTTCATCCGAGCAAGTGAAACGCAAGAATATATTATTTCCGAGGCAGAGGCTGCTTTGAAGGCCCATATAAAAGACCCAATATTTCATAGGGTTAGGGATGTTGCTCCAAACAAGGCAATGTTTTGCTTAAGCTTTAGGCTGCCAGAAGCGTGCCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_10

Protein Analysis

499

Amino Acids

56.36

Weight (kDa)

6.23

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW2_N_2 PF25133 132 - 187 6.4e-14 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 191 - 290 1.1e-24 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016610)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56120
fragaria_vesca FvH4_1g05380
malus_domestica MD02G1062400.v1.1
pyrus_communis pycom02g04360
rosa_chinensis RchiOBHm_Chr2g0091071
rosa_laevigata RLG00000016174
rosa_multiflora Rmu_sc0005171.1_g000009
rosa_roxburghii Rroxscaffold_2G00150620
rosa_rugosa Rorug02G0012300
rosa_samantha Rh2AG058400 Rh2BG056800 Rh2CG059000 Rh2DG058300
rosa_wichuraiana Rw2G005080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1099
AccI GTMKAC 1 cut(s) 1039
AccII CGCG 1 cut(s) 528
AccIII TCCGGA 2 cut(s) 208, 772
AciI CCGC 1 cut(s) 732
AclI AACGTT 2 cut(s) 990, 1137
AclWI GGATC 2 cut(s) 514, 1282
AcsI RAATTY 5 cut(s) 17, 628, 857, 904, 1262
AcuI CTGAAG 3 cut(s) 251, 812, 1053
AfaI GTAC 5 cut(s) 281, 368, 943, 1008, 1074
AfiI CCNNNNNNNGG 2 cut(s) 331, 890
AflII CTTAAG 1 cut(s) 1465
AflIII ACRYGT 2 cut(s) 143, 938
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AloI GAACNNNNNNTCC 2 cut(s) 321, 353
AluBI AGCT 7 cut(s) 64, 374, 604, 615, 677, 1148, 1470
AluI AGCT 7 cut(s) 64, 374, 604, 615, 677, 1148, 1470
Alw26I GTCTC 1 cut(s) 929
AlwI GGATC 2 cut(s) 514, 1282
AlwNI CAGNNNCTG 3 cut(s) 319, 884, 1388
Ama87I CYCGRG 1 cut(s) 275
Aor13HI TCCGGA 2 cut(s) 208, 772
AoxI GGCC 2 cut(s) 712, 1398
ApeKI GCWGC 2 cut(s) 1388, 1477
ApoI RAATTY 5 cut(s) 17, 628, 857, 904, 1262
Asp700I GAANNNNTTC 2 cut(s) 255, 1314
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 3 cut(s) 136, 713, 1399
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 833, 1297
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 136
BauI CACGAG 1 cut(s) 57
BbvI GCAGC 2 cut(s) 1375, 1464
BccI CCATC 2 cut(s) 411, 868
BciVI GTATCC 1 cut(s) 205
BclI TGATCA 2 cut(s) 1197, 1225
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 929
BfaI CTAG 3 cut(s) 1010, 1050, 1268
BfmI CTRYAG 2 cut(s) 610, 1092
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 1465
BfuAI ACCTGC 1 cut(s) 1099
BfuI GTATCC 1 cut(s) 205
BisI GCNGC 3 cut(s) 732, 1389, 1478
BlsI GCNGC 3 cut(s) 733, 1390, 1479
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 3 cut(s) 136, 713, 1399
BmiI GGNNCC 2 cut(s) 138, 339
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 6 cut(s) 734, 756, 838, 901, 1054, 1261
BoxI GACNNNNGTC 1 cut(s) 491
BplI GAGNNNNNCTC 2 cut(s) 1297, 1329
BpmI CTGGAG 1 cut(s) 1062
BpuMI CCSGG 1 cut(s) 327
BsaJI CCNNGG 2 cut(s) 325, 1377
BsaWI WCCGGW 2 cut(s) 208, 772
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 890
Bse1I ACTGG 1 cut(s) 1079
Bse3DI GCAATG 2 cut(s) 982, 1461
BseAI TCCGGA 2 cut(s) 208, 772
BseDI CCNNGG 2 cut(s) 325, 1377
BseGI GGATG 5 cut(s) 13, 445, 749, 1341, 1441
BseLI CCNNNNNNNGG 2 cut(s) 331, 890
BseMI GCAATG 2 cut(s) 982, 1461
BseMII CTCAG 3 cut(s) 384, 680, 1182
BseNI ACTGG 1 cut(s) 1079
BseXI GCAGC 2 cut(s) 1375, 1464
BseYI CCCAGC 1 cut(s) 677
BsgI GTGCAG 1 cut(s) 315
Bsh1236I CGCG 1 cut(s) 528
BshFI GGCC 2 cut(s) 714, 1400
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 4 cut(s) 209, 327, 773, 1247
BslI CCNNNNNNNGG 2 cut(s) 331, 890
BsmAI GTCTC 1 cut(s) 929
BsmI GAATGC 2 cut(s) 780, 987
BsnI GGCC 2 cut(s) 714, 1400
BsoBI CYCGRG 1 cut(s) 275
Bsp13I TCCGGA 2 cut(s) 208, 772
Bsp143I GATC 5 cut(s) 506, 795, 1197, 1225, 1287
BspACI CCGC 1 cut(s) 732
BspANI GGCC 2 cut(s) 714, 1400
BspCNI CTCAG 3 cut(s) 383, 679, 1183
BspEI TCCGGA 2 cut(s) 208, 772
BspFNI CGCG 1 cut(s) 528
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 138, 339
BspMAI CTGCAG 1 cut(s) 1096
BspMI ACCTGC 1 cut(s) 1099
BspPI GGATC 2 cut(s) 514, 1282
BspTI CTTAAG 1 cut(s) 1465
BsrDI GCAATG 2 cut(s) 982, 1461
BsrI ACTGG 1 cut(s) 1079
BssECI CCNNGG 2 cut(s) 325, 1377
BssMI GATC 5 cut(s) 506, 795, 1197, 1225, 1287
BssSI CACGAG 1 cut(s) 57
Bst2BI CACGAG 1 cut(s) 57
Bst4CI ACNGT 3 cut(s) 158, 890, 1037
Bst6I CTCTTC 1 cut(s) 1320
BstAFI CTTAAG 1 cut(s) 1465
BstAPI GCANNNNNTGC 2 cut(s) 319, 1388
BstC8I GCNNGC 2 cut(s) 530, 1489
BstDEI CTNAG 3 cut(s) 370, 666, 1191
BstEII GGTNACC 1 cut(s) 821
BstF5I GGATG 5 cut(s) 13, 445, 749, 1341, 1441
BstFNI CGCG 1 cut(s) 528
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 5 cut(s) 509, 798, 1200, 1228, 1290
BstMAI GTCTC 1 cut(s) 929
BstMBI GATC 5 cut(s) 506, 795, 1197, 1225, 1287
BstMWI GCNNNNNNNGC 4 cut(s) 319, 728, 1388, 1397
BstNSI RCATGY 3 cut(s) 147, 942, 1223
BstPAI GACNNNNGTC 1 cut(s) 491
BstPI GGTNACC 1 cut(s) 821
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 2 cut(s) 610, 1092
BstUI CGCG 1 cut(s) 528
BstV1I GCAGC 2 cut(s) 1375, 1464
BstX2I RGATCY 1 cut(s) 506
BstYI RGATCY 1 cut(s) 506
BsuI GTATCC 1 cut(s) 205
BsuRI GGCC 2 cut(s) 714, 1400
BtsCI GGATG 5 cut(s) 13, 445, 749, 1341, 1441
BtsI GCAGTG 2 cut(s) 885, 1327
BtsIMutI CAGTG 2 cut(s) 885, 1327
BveI ACCTGC 1 cut(s) 1099
Cac8I GCNNGC 2 cut(s) 530, 1489
CaiI CAGNNNCTG 3 cut(s) 319, 884, 1388
CciI TCATGA 1 cut(s) 376
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 3 cut(s) 136, 713, 1399
CseI GACGC 1 cut(s) 903
Csp6I GTAC 5 cut(s) 280, 367, 942, 1007, 1073
CviQI GTAC 5 cut(s) 280, 367, 942, 1007, 1073
DdeI CTNAG 3 cut(s) 370, 666, 1191
DpnI GATC 5 cut(s) 508, 797, 1199, 1227, 1289
DpnII GATC 5 cut(s) 506, 795, 1197, 1225, 1287
DraI TTTAAA 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 1320
EarI CTCTTC 1 cut(s) 1320
Eco47I GGWCC 1 cut(s) 136
Eco57I CTGAAG 3 cut(s) 251, 812, 1053
Eco88I CYCGRG 1 cut(s) 275
Eco91I GGTNACC 1 cut(s) 821
EcoO109I RGGNCCY 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 821
EcoRI GAATTC 2 cut(s) 628, 904
EcoT22I ATGCAT 2 cut(s) 749, 1276
FalI AAGNNNNNCTT 1 cut(s) 1476
FauNDI CATATG 1 cut(s) 689
FbaI TGATCA 2 cut(s) 1197, 1225
FblI GTMKAC 1 cut(s) 1039
Fnu4HI GCNGC 3 cut(s) 732, 1389, 1478
FokI GGATG 5 cut(s) 20, 452, 756, 1328, 1448
Fsp4HI GCNGC 3 cut(s) 732, 1389, 1478
FspBI CTAG 3 cut(s) 1010, 1050, 1268
GlaI GCGC 1 cut(s) 45
GluI GCNGC 3 cut(s) 732, 1389, 1478
GsaI CCCAGC 1 cut(s) 681
GsuI CTGGAG 1 cut(s) 1062
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 2 cut(s) 714, 1400
HapII CCGG 4 cut(s) 209, 327, 773, 1247
HgaI GACGC 1 cut(s) 903
HhaI GCGC 1 cut(s) 46
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HindIII AAGCTT 2 cut(s) 602, 1468
HpaII CCGG 4 cut(s) 209, 327, 773, 1247
HphI GGTGA 2 cut(s) 833, 1297
Hpy166II GTNNAC 3 cut(s) 270, 1033, 1040
Hpy8I GTNNAC 3 cut(s) 270, 1033, 1040
HpyAV CCTTC 4 cut(s) 226, 351, 1253, 1390
HpyCH4III ACNGT 3 cut(s) 158, 890, 1037
HpyCH4IV ACGT 3 cut(s) 396, 990, 1137
HpyF10VI GCNNNNNNNGC 4 cut(s) 319, 728, 1388, 1397
HpyF3I CTNAG 3 cut(s) 370, 666, 1191
HpySE526I ACGT 3 cut(s) 396, 990, 1137
HspAI GCGC 1 cut(s) 44
Kpn2I TCCGGA 2 cut(s) 208, 772
Ksp22I TGATCA 2 cut(s) 1197, 1225
Kzo9I GATC 5 cut(s) 506, 795, 1197, 1225, 1287
LmnI GCTCC 4 cut(s) 590, 1155, 1216, 1447
Lsp1109I GCAGC 2 cut(s) 1375, 1464
LweI GCATC 6 cut(s) 734, 756, 838, 901, 1054, 1261
MaeI CTAG 3 cut(s) 1010, 1050, 1268
MaeII ACGT 3 cut(s) 396, 990, 1137
MaeIII GTNAC 5 cut(s) 158, 392, 568, 821, 1018
MalI GATC 5 cut(s) 508, 797, 1199, 1227, 1289
MboI GATC 5 cut(s) 506, 795, 1197, 1225, 1287
MboII GAAGA 2 cut(s) 805, 1307
MfeI CAATTG 2 cut(s) 489, 869
MflI RGATCY 1 cut(s) 506
MlyI GAGTC 2 cut(s) 265, 400
MmeI TCCRAC 2 cut(s) 216, 484
Mph1103I ATGCAT 2 cut(s) 749, 1276
MroI TCCGGA 2 cut(s) 208, 772
MroXI GAANNNNTTC 2 cut(s) 255, 1314
MspCI CTTAAG 1 cut(s) 1465
MspI CCGG 4 cut(s) 209, 327, 773, 1247
MspR9I CCNGG 1 cut(s) 327
MunI CAATTG 2 cut(s) 489, 869
Mva1269I GAATGC 2 cut(s) 780, 987
MvnI CGCG 1 cut(s) 528
MwoI GCNNNNNNNGC 4 cut(s) 319, 728, 1388, 1397
NciI CCSGG 1 cut(s) 327
NdeI CATATG 1 cut(s) 689
NdeII GATC 5 cut(s) 506, 795, 1197, 1225, 1287
NlaIV GGNNCC 2 cut(s) 138, 339
NmuCI GTSAC 2 cut(s) 392, 821
NsiI ATGCAT 2 cut(s) 749, 1276
NspI RCATGY 3 cut(s) 147, 942, 1223
PacI TTAATTAA 1 cut(s) 1498
PagI TCATGA 1 cut(s) 376
PciI ACATGT 2 cut(s) 143, 938
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PctI GAATGC 2 cut(s) 780, 987
PdmI GAANNNNTTC 2 cut(s) 255, 1314
PfeI GAWTC 8 cut(s) 52, 188, 316, 380, 480, 962, 968, 1324
PkrI GCNGC 3 cut(s) 733, 1390, 1479
PleI GAGTC 2 cut(s) 265, 399
PpsI GAGTC 2 cut(s) 265, 399
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 2 cut(s) 143, 938
PshAI GACNNNNGTC 1 cut(s) 491
Psp1406I AACGTT 2 cut(s) 990, 1137
Psp5II RGGWCCY 1 cut(s) 136
PspEI GGTNACC 1 cut(s) 821
PspFI CCCAGC 1 cut(s) 677
PspN4I GGNNCC 2 cut(s) 138, 339
PspPI GGNCC 3 cut(s) 136, 713, 1399
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 1096
PstNI CAGNNNCTG 3 cut(s) 319, 884, 1388
PsuI RGATCY 1 cut(s) 506
RsaI GTAC 5 cut(s) 281, 368, 943, 1008, 1074
RsaNI GTAC 5 cut(s) 280, 367, 942, 1007, 1073
SatI GCNGC 3 cut(s) 732, 1389, 1478
Sau3AI GATC 5 cut(s) 506, 795, 1197, 1225, 1287
Sau96I GGNCC 3 cut(s) 136, 713, 1399
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 2 cut(s) 265, 400
ScrFI CCNGG 1 cut(s) 327
SfaNI GCATC 6 cut(s) 734, 756, 838, 901, 1054, 1261
SfcI CTRYAG 2 cut(s) 610, 1092
SinI GGWCC 1 cut(s) 136
SmlI CTYRAG 1 cut(s) 1465
SmoI CTYRAG 1 cut(s) 1465
SsiI CCGC 1 cut(s) 732
SspI AATATT 2 cut(s) 1301, 1419
SspMI CTAG 3 cut(s) 1010, 1050, 1268
StyD4I CCNGG 1 cut(s) 325
TaaI ACNGT 3 cut(s) 158, 890, 1037
TaiI ACGT 3 cut(s) 399, 993, 1140
TaqI TCGA 2 cut(s) 82, 673
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 734
TfiI GAWTC 8 cut(s) 52, 188, 316, 380, 480, 962, 968, 1324
TscAI CASTG 2 cut(s) 885, 1334
TseFI GTSAC 2 cut(s) 392, 821
TseI GCWGC 2 cut(s) 1388, 1477
Tsp45I GTSAC 2 cut(s) 392, 821
TspDTI ATGAA 7 cut(s) 240, 333, 660, 773, 1253, 1330, 1412
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 2 cut(s) 885, 1334
Vha464I CTTAAG 1 cut(s) 1465
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 5 cut(s) 17, 628, 857, 904, 1262
XbaI TCTAGA 1 cut(s) 1267
XceI RCATGY 3 cut(s) 147, 942, 1223
XmiI GTMKAC 1 cut(s) 1039
XmnI GAANNNNTTC 2 cut(s) 255, 1314
XspI CTAG 3 cut(s) 1010, 1050, 1268
ZrmI AGTACT 1 cut(s) 368
Zsp2I ATGCAT 2 cut(s) 749, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.