RchiOBHm_Chr2g0107141

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0019884
Physical Location & Seq
Reverse (-)
18508668 .. 18509889
1222 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGAAGGGAAAAATAACACACACGCACTCTCTGTTTCTCTCTGTGTTCCTCTCGTCCTGCATATCGAAGGAGCTCAAATCCAAAGTGAGCCCAACACCTGCAGTAGTAGATTTATTGCTACAAATCGAGCAATCAAAAGGTGGAGGAGATCCAGCCTGGCACGAGCGGCTAAACCCTGACCGTCAAGGTCGTCACCACCAATCTGGTCGAGAGGTCACCTCTAAGAATGGCGGTGCTCGATCCTCTCTCTCGTCTCTCCCTCTTCAGCCCCCTCGCATCACTGAGTGCCTCGTTAGCGATGAGACTAGAACCATTGTCTTCACTGCTCGCAATGACCAAGTTGACATTATGAAGCCTAGTACCACTGTGATTCCATGCAATACAAAGATTGACATGTTCATCAAAGGCACTATGAGGCTTGCAGTTGATAAATGGGGTCGTGTTGAAGTCACAGAACCAGCCAACTTTGAAGTTAAGGAGGTCAACAATCTTTCTCTTGTCGAGTATGAATTACTTAGTGTTGCTGAATGA

Protein Analysis

176

Amino Acids

19.53

Weight (kDa)

7.76

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 71 - 150 1.8e-27 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019884)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0107141 RchiOBHm_Chr5g0050941
rosa_multiflora Rmu_sc0002539.1_g000047 Rmu_sc0002676.1_g000003
rosa_roxburghii Rroxscaffold_3G00239490
rosa_rugosa Rorug05G0382400
rosa_samantha Rh5DG359800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 106
AccBSI CCGCTC 1 cut(s) 166
AciI CCGC 2 cut(s) 166, 231
AclWI GGATC 2 cut(s) 143, 234
AcuI CTGAAG 1 cut(s) 248
AdeI CACNNNGTG 1 cut(s) 285
AfaI GTAC 1 cut(s) 361
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 2 cut(s) 446, 470
AjnI CCWGG 1 cut(s) 155
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
Alw21I GWGCWC 2 cut(s) 75, 238
Alw26I GTCTC 2 cut(s) 258, 296
AlwI GGATC 2 cut(s) 143, 234
AsuHPI GGTGA 2 cut(s) 185, 208
BanII GRGCYC 2 cut(s) 75, 92
BauI CACGAG 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 2 cut(s) 75, 238
BciT130I CCWGG 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 258, 296
BfaI CTAG 2 cut(s) 306, 357
BfmI CTRYAG 1 cut(s) 99
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 168
Bme1390I CCNGG 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 310
BplI GAGNNNNNCTC 2 cut(s) 203, 235
Bse3DI GCAATG 1 cut(s) 337
BseBI CCWGG 1 cut(s) 157
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 273
BseRI GAGGAG 1 cut(s) 159
BsiHKAI GWGCWC 2 cut(s) 75, 238
BsmAI GTCTC 2 cut(s) 258, 296
BsmBI CGTCTC 1 cut(s) 258
Bsp1286I GDGCHC 3 cut(s) 75, 92, 238
Bsp143I GATC 2 cut(s) 148, 239
BspACI CCGC 2 cut(s) 166, 231
BspCNI CTCAG 1 cut(s) 274
BspMAI CTGCAG 1 cut(s) 103
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 2 cut(s) 143, 234
BsrBI CCGCTC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 337
BssMI GATC 2 cut(s) 148, 239
BssSI CACGAG 1 cut(s) 161
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 1 cut(s) 157
Bst4CI ACNGT 2 cut(s) 182, 367
Bst6I CTCTTC 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 328, 420
BstDEI CTNAG 3 cut(s) 222, 282, 515
BstEII GGTNACC 1 cut(s) 214
BstKTI GATC 2 cut(s) 151, 242
BstMAI GTCTC 2 cut(s) 258, 296
BstMBI GATC 2 cut(s) 148, 239
BstMWI GCNNNNNNNGC 2 cut(s) 166, 294
BstNI CCWGG 1 cut(s) 157
BstNSI RCATGY 1 cut(s) 397
BstPI GGTNACC 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 155
BstSFI CTRYAG 1 cut(s) 99
BstV2I GAAGAC 1 cut(s) 310
BstX2I RGATCY 1 cut(s) 148
BstXI CCANNNNNNTGG 1 cut(s) 203
BstYI RGATCY 1 cut(s) 148
BtgZI GCGATG 1 cut(s) 312
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 3 cut(s) 279, 321, 363
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 328, 420
Csp6I GTAC 1 cut(s) 360
CviAII CATG 2 cut(s) 375, 394
CviJI RGCY 8 cut(s) 73, 90, 155, 169, 268, 355, 418, 461
CviKI_1 RGCY 8 cut(s) 73, 90, 155, 169, 268, 355, 418, 461
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 3 cut(s) 222, 282, 515
DpnI GATC 2 cut(s) 150, 241
DpnII GATC 2 cut(s) 148, 239
DraIII CACNNNGTG 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 73
Eco24I GRGCYC 2 cut(s) 75, 92
Eco53kI GAGCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 248
Eco91I GGTNACC 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 73
EcoO65I GGTNACC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 155
EcoT38I GRGCYC 2 cut(s) 75, 92
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 2 cut(s) 378, 397
FaiI YATR 6 cut(s) 62, 350, 376, 395, 413, 507
FatI CATG 2 cut(s) 374, 393
Fnu4HI GCNGC 1 cut(s) 167
FriOI GRGCYC 2 cut(s) 75, 92
Fsp4HI GCNGC 1 cut(s) 167
FspBI CTAG 2 cut(s) 306, 357
GluI GCNGC 1 cut(s) 167
Hin1II CATG 2 cut(s) 378, 397
HincII GTYRAC 2 cut(s) 343, 484
HindII GTYRAC 2 cut(s) 343, 484
HinfI GANTC 1 cut(s) 370
HphI GGTGA 2 cut(s) 185, 208
Hpy166II GTNNAC 2 cut(s) 343, 484
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 2 cut(s) 343, 484
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 2 cut(s) 182, 367
HpyCH4V TGCA 4 cut(s) 60, 101, 378, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 294
HpyF3I CTNAG 3 cut(s) 222, 282, 515
Hsp92II CATG 2 cut(s) 378, 397
Kzo9I GATC 2 cut(s) 148, 239
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 8 cut(s) 70, 111, 142, 165, 169, 189, 189, 471
LweI GCATC 1 cut(s) 285
MaeI CTAG 2 cut(s) 306, 357
MaeIII GTNAC 3 cut(s) 191, 214, 448
MalI GATC 2 cut(s) 150, 241
MbiI CCGCTC 1 cut(s) 166
MboI GATC 2 cut(s) 148, 239
MboII GAAGA 2 cut(s) 254, 310
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 3 cut(s) 75, 92, 238
MluCI AATT 1 cut(s) 509
MseI TTAA 1 cut(s) 474
MspR9I CCNGG 1 cut(s) 157
MvaI CCWGG 1 cut(s) 157
MwoI GCNNNNNNNGC 2 cut(s) 166, 294
NdeII GATC 2 cut(s) 148, 239
NlaIII CATG 2 cut(s) 378, 397
NmuCI GTSAC 3 cut(s) 191, 214, 448
NspI RCATGY 1 cut(s) 397
PaqCI CACCTGC 1 cut(s) 106
PciI ACATGT 1 cut(s) 393
PfeI GAWTC 1 cut(s) 370
PkrI GCNGC 1 cut(s) 168
PscI ACATGT 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 155
PspEI GGTNACC 1 cut(s) 214
PspGI CCWGG 1 cut(s) 155
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 1 cut(s) 148
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 1 cut(s) 167
Sau3AI GATC 2 cut(s) 148, 239
ScrFI CCNGG 1 cut(s) 157
SduI GDGCHC 3 cut(s) 75, 92, 238
SetI ASST 7 cut(s) 75, 100, 142, 190, 216, 221, 483
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 1 cut(s) 99
Sse9I AATT 1 cut(s) 509
SsiI CCGC 2 cut(s) 166, 231
SspMI CTAG 2 cut(s) 306, 357
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 155
TaaI ACNGT 2 cut(s) 182, 367
TaqI TCGA 5 cut(s) 65, 126, 208, 238, 501
TasI AATT 1 cut(s) 509
TauI GCSGC 1 cut(s) 169
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 3 cut(s) 286, 328, 370
TseFI GTSAC 3 cut(s) 191, 214, 448
Tsp45I GTSAC 3 cut(s) 191, 214, 448
TspDTI ATGAA 4 cut(s) 17, 365, 388, 522
TspRI CASTG 3 cut(s) 286, 328, 370
XceI RCATGY 1 cut(s) 397
XspI CTAG 2 cut(s) 306, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.