Rh5DG359800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0019884
Physical Location & Seq
Forward (+)
53741229 .. 53747974
6746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG359800.1

Sequence Viewer

Length: 492 bp
ATGCCACCCCTCAAAAGTATAACCTACAATCTGTTTCCCATACAGGCGAGCCCAGCACCTGCAGTAGTAGATTTATTGCTACAAATCGAGCAATCAAAAGGTGGAGGAGATCCAGCCTGGCACGAGCGGCTAAACCCTGACCGTCAAGGTCGTCACCACCAATCTGGTCGAGAGGTCACCTCCAAGAATGGCGGTGCTCGATCCTCTCTCTCGTCTCTCCCTCTTCAGCCCCCTCGCATCACTGAGTGCCTCGTTAGCGATGAGACTGGAACCATTGTCTTCACTGCTCGCAATGACCAAGTTGACATTATGAAGCCTAGTACCACTGTGATTCCACGCAATACAAAGATTGACATGTTCATCAAAGGCACTATGAGGCTTGCAGTTGATAAATGGGGTTGTGTTGAAGTCACAGAACCAGCCAGCTTTGAAGTTAAAGAGGTCAACAATCTTTCTCTTGTCGAGTATGAATTACTTAGTGTTGCTGAATGA

Protein Analysis

163

Amino Acids

17.95

Weight (kDa)

5.66

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 58 - 137 3.9e-29 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019884)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0107141 RchiOBHm_Chr5g0050941
rosa_multiflora Rmu_sc0002539.1_g000047 Rmu_sc0002676.1_g000003
rosa_roxburghii Rroxscaffold_3G00239490
rosa_rugosa Rorug05G0382400
rosa_samantha Rh5DG359800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 67
Acc36I ACCTGC 1 cut(s) 67
AccBSI CCGCTC 1 cut(s) 127
AciI CCGC 2 cut(s) 127, 192
AclWI GGATC 2 cut(s) 104, 195
AcuI CTGAAG 1 cut(s) 209
AdeI CACNNNGTG 1 cut(s) 246
AfaI GTAC 1 cut(s) 322
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 2 cut(s) 407, 431
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 1 cut(s) 426
AluI AGCT 1 cut(s) 426
Alw21I GWGCWC 1 cut(s) 199
Alw26I GTCTC 2 cut(s) 219, 257
AlwI GGATC 2 cut(s) 104, 195
AlwNI CAGNNNCTG 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 146, 169
BanII GRGCYC 1 cut(s) 53
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 199
BciT130I CCWGG 1 cut(s) 118
BcoDI GTCTC 2 cut(s) 219, 257
BfaI CTAG 1 cut(s) 318
BfmI CTRYAG 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 67
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 271
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bse1I ACTGG 1 cut(s) 271
Bse3DI GCAATG 1 cut(s) 298
BseBI CCWGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 120
BseYI CCCAGC 1 cut(s) 52
BsiHKAI GWGCWC 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 219, 257
BsmBI CGTCTC 1 cut(s) 219
Bsp1286I GDGCHC 2 cut(s) 53, 199
Bsp143I GATC 2 cut(s) 109, 200
BspACI CCGC 2 cut(s) 127, 192
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 64
BspMI ACCTGC 1 cut(s) 67
BspPI GGATC 2 cut(s) 104, 195
BsrBI CCGCTC 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 298
BsrI ACTGG 1 cut(s) 271
BssMI GATC 2 cut(s) 109, 200
BssSI CACGAG 1 cut(s) 122
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 143, 328
Bst6I CTCTTC 1 cut(s) 228
BstC8I GCNNGC 4 cut(s) 49, 289, 381, 424
BstDEI CTNAG 2 cut(s) 243, 476
BstEII GGTNACC 1 cut(s) 175
BstKTI GATC 2 cut(s) 112, 203
BstMAI GTCTC 2 cut(s) 219, 257
BstMBI GATC 2 cut(s) 109, 200
BstMWI GCNNNNNNNGC 3 cut(s) 53, 127, 255
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 1 cut(s) 358
BstPI GGTNACC 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 60
BstV2I GAAGAC 1 cut(s) 271
BstX2I RGATCY 1 cut(s) 109
BstXI CCANNNNNNTGG 1 cut(s) 164
BstYI RGATCY 1 cut(s) 109
BtgZI GCGATG 1 cut(s) 273
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 3 cut(s) 240, 282, 324
BveI ACCTGC 1 cut(s) 67
Cac8I GCNNGC 4 cut(s) 49, 289, 381, 424
CaiI CAGNNNCTG 1 cut(s) 59
Csp6I GTAC 1 cut(s) 321
CviAII CATG 1 cut(s) 355
CviJI RGCY 8 cut(s) 51, 116, 130, 229, 316, 379, 422, 426
CviKI_1 RGCY 8 cut(s) 51, 116, 130, 229, 316, 379, 422, 426
CviQI GTAC 1 cut(s) 321
DdeI CTNAG 2 cut(s) 243, 476
DpnI GATC 2 cut(s) 111, 202
DpnII GATC 2 cut(s) 109, 200
DraIII CACNNNGTG 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 53
Eco57I CTGAAG 1 cut(s) 209
Eco91I GGTNACC 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 116
EcoT38I GRGCYC 1 cut(s) 53
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 1 cut(s) 358
FaiI YATR 6 cut(s) 20, 41, 311, 356, 374, 468
FatI CATG 1 cut(s) 354
Fnu4HI GCNGC 1 cut(s) 128
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 128
FspBI CTAG 1 cut(s) 318
GluI GCNGC 1 cut(s) 128
GsaI CCCAGC 1 cut(s) 56
Hin1II CATG 1 cut(s) 358
HincII GTYRAC 2 cut(s) 304, 445
HindII GTYRAC 2 cut(s) 304, 445
HinfI GANTC 1 cut(s) 331
HphI GGTGA 2 cut(s) 146, 169
Hpy166II GTNNAC 2 cut(s) 304, 445
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 2 cut(s) 304, 445
HpyCH4III ACNGT 2 cut(s) 143, 328
HpyCH4V TGCA 2 cut(s) 62, 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 127, 255
HpyF3I CTNAG 2 cut(s) 243, 476
Hsp92II CATG 1 cut(s) 358
Kzo9I GATC 2 cut(s) 109, 200
LweI GCATC 1 cut(s) 246
MaeI CTAG 1 cut(s) 318
MaeIII GTNAC 3 cut(s) 152, 175, 409
MalI GATC 2 cut(s) 111, 202
MbiI CCGCTC 1 cut(s) 127
MboI GATC 2 cut(s) 109, 200
MboII GAAGA 2 cut(s) 215, 271
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 53, 199
MluCI AATT 1 cut(s) 470
MseI TTAA 1 cut(s) 435
MspR9I CCNGG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 3 cut(s) 53, 127, 255
NdeII GATC 2 cut(s) 109, 200
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 1 cut(s) 271
NmuCI GTSAC 3 cut(s) 152, 175, 409
NspI RCATGY 1 cut(s) 358
PaqCI CACCTGC 1 cut(s) 67
PciI ACATGT 1 cut(s) 354
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 1 cut(s) 129
PscI ACATGT 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 116
PspEI GGTNACC 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 271
PstI CTGCAG 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 59
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 1 cut(s) 322
RsaNI GTAC 1 cut(s) 321
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 2 cut(s) 109, 200
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 2 cut(s) 53, 199
SetI ASST 8 cut(s) 26, 61, 103, 151, 177, 182, 428, 444
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 1 cut(s) 60
Sse9I AATT 1 cut(s) 470
SsiI CCGC 2 cut(s) 127, 192
SspMI CTAG 1 cut(s) 318
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 2 cut(s) 143, 328
TaqI TCGA 4 cut(s) 87, 169, 199, 462
TasI AATT 1 cut(s) 470
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 3 cut(s) 247, 289, 331
TseFI GTSAC 3 cut(s) 152, 175, 409
Tsp45I GTSAC 3 cut(s) 152, 175, 409
TspDTI ATGAA 3 cut(s) 326, 349, 483
TspRI CASTG 3 cut(s) 247, 289, 331
XceI RCATGY 1 cut(s) 358
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.