Rroxscaffold_3G00239490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0019884
Physical Location & Seq
Reverse (-)
29858113 .. 29859220
1108 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00239490.1

Sequence Viewer

Length: 450 bp
ATGAATAGCCCAGCACCTGCAGTAGTAGATTTATTGCTACAAATCGAGCAATTAAAAGGTGGAGGAGATCCAGCCTGGCACGAGCGGCTAAACCCTGACCGTCAAGGTCGACACCACCAATCTGGTCGAGAGGTCACCTCCAAGAATGGCGGTGCTCGATCCTCTCTCTCCTCTCTCCCTCTTCAGCCCCCTCGCATCACTGAGTGCCTCGTTAGCGATGAGACTGGAACCATTGTCTTCACTGCTCGCAATGACCAAGTTGACATTATGAAGCCTAGTACCACTGTGATTCCACGCAATACAAAGATTGACATGTTCATCAAAGGCACTATGAGGCTTGCAGTTGATAAATGGGGTCGTGTTGAAGTCACAGAACCAGCCAGCTTTGAAGTTAAGGAGGTCAACAATCTTTCTCTTGTCGAGTATGAATTACTTAGTGTTGCTGAATGA

Protein Analysis

149

Amino Acids

16.46

Weight (kDa)

5.66

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 44 - 123 3.8e-30 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019884)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0107141 RchiOBHm_Chr5g0050941
rosa_multiflora Rmu_sc0002539.1_g000047 Rmu_sc0002676.1_g000003
rosa_roxburghii Rroxscaffold_3G00239490
rosa_rugosa Rorug05G0382400
rosa_samantha Rh5DG359800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 25
Acc36I ACCTGC 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 85
AccI GTMKAC 1 cut(s) 109
AciI CCGC 2 cut(s) 85, 150
AclWI GGATC 2 cut(s) 62, 153
AcuI CTGAAG 1 cut(s) 167
AdeI CACNNNGTG 1 cut(s) 204
AfaI GTAC 1 cut(s) 280
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 2 cut(s) 365, 389
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 1 cut(s) 384
AluI AGCT 1 cut(s) 384
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 2 cut(s) 62, 153
AlwNI CAGNNNCTG 1 cut(s) 17
AsuHPI GGTGA 1 cut(s) 127
BauI CACGAG 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 157
BciT130I CCWGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 1 cut(s) 276
BfmI CTRYAG 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 25
BisI GCNGC 1 cut(s) 86
BlsI GCNGC 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 76
BmiI GGNNCC 1 cut(s) 229
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 204
BpiI GAAGAC 1 cut(s) 229
BplI GAGNNNNNCTC 2 cut(s) 122, 154
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 256
BseBI CCWGG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 229
BseRI GAGGAG 2 cut(s) 78, 160
BseYI CCCAGC 1 cut(s) 10
BsiHKAI GWGCWC 1 cut(s) 157
BsmAI GTCTC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 2 cut(s) 67, 158
BspACI CCGC 2 cut(s) 85, 150
BspCNI CTCAG 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 229
BspMAI CTGCAG 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 25
BspPI GGATC 2 cut(s) 62, 153
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 256
BsrI ACTGG 1 cut(s) 229
BssMI GATC 2 cut(s) 67, 158
BssSI CACGAG 1 cut(s) 80
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 2 cut(s) 101, 286
Bst6I CTCTTC 1 cut(s) 186
BstC8I GCNNGC 3 cut(s) 247, 339, 382
BstDEI CTNAG 2 cut(s) 201, 434
BstEII GGTNACC 1 cut(s) 133
BstKTI GATC 2 cut(s) 70, 161
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 2 cut(s) 67, 158
BstMWI GCNNNNNNNGC 2 cut(s) 85, 213
BstNI CCWGG 1 cut(s) 76
BstNSI RCATGY 1 cut(s) 316
BstPI GGTNACC 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 74
BstSFI CTRYAG 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 67
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 67
BtgZI GCGATG 1 cut(s) 231
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 3 cut(s) 198, 240, 282
BveI ACCTGC 1 cut(s) 25
Cac8I GCNNGC 3 cut(s) 247, 339, 382
CaiI CAGNNNCTG 1 cut(s) 17
Csp6I GTAC 1 cut(s) 279
CviAII CATG 1 cut(s) 313
CviJI RGCY 8 cut(s) 9, 74, 88, 187, 274, 337, 380, 384
CviKI_1 RGCY 8 cut(s) 9, 74, 88, 187, 274, 337, 380, 384
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 2 cut(s) 201, 434
DpnI GATC 2 cut(s) 69, 160
DpnII GATC 2 cut(s) 67, 158
DraIII CACNNNGTG 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 74
FaeI CATG 1 cut(s) 316
FaiI YATR 4 cut(s) 269, 314, 332, 426
FatI CATG 1 cut(s) 312
FblI GTMKAC 1 cut(s) 109
Fnu4HI GCNGC 1 cut(s) 86
Fsp4HI GCNGC 1 cut(s) 86
FspBI CTAG 1 cut(s) 276
GluI GCNGC 1 cut(s) 86
GsaI CCCAGC 1 cut(s) 14
Hin1II CATG 1 cut(s) 316
HincII GTYRAC 3 cut(s) 110, 262, 403
HindII GTYRAC 3 cut(s) 110, 262, 403
HinfI GANTC 1 cut(s) 289
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 3 cut(s) 110, 262, 403
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 3 cut(s) 110, 262, 403
HpyCH4III ACNGT 2 cut(s) 101, 286
HpyCH4V TGCA 2 cut(s) 20, 341
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 213
HpyF3I CTNAG 2 cut(s) 201, 434
Hsp92II CATG 1 cut(s) 316
Kzo9I GATC 2 cut(s) 67, 158
LweI GCATC 1 cut(s) 204
MaeI CTAG 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 133, 367
MalI GATC 2 cut(s) 69, 160
MbiI CCGCTC 1 cut(s) 85
MboI GATC 2 cut(s) 67, 158
MboII GAAGA 2 cut(s) 173, 229
MflI RGATCY 1 cut(s) 67
MhlI GDGCHC 1 cut(s) 157
MluCI AATT 2 cut(s) 50, 428
MseI TTAA 2 cut(s) 53, 393
MspR9I CCNGG 1 cut(s) 76
MvaI CCWGG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 85, 213
NdeII GATC 2 cut(s) 67, 158
NlaIII CATG 1 cut(s) 316
NlaIV GGNNCC 1 cut(s) 229
NmuCI GTSAC 2 cut(s) 133, 367
NspI RCATGY 1 cut(s) 316
PaqCI CACCTGC 1 cut(s) 25
PciI ACATGT 1 cut(s) 312
PfeI GAWTC 1 cut(s) 289
PkrI GCNGC 1 cut(s) 87
PscI ACATGT 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 74
PspEI GGTNACC 1 cut(s) 133
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 229
PstI CTGCAG 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 67
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
SalI GTCGAC 1 cut(s) 108
SaqAI TTAA 2 cut(s) 53, 393
SatI GCNGC 1 cut(s) 86
Sau3AI GATC 2 cut(s) 67, 158
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 1 cut(s) 157
SetI ASST 7 cut(s) 19, 61, 109, 135, 140, 386, 402
SfaNI GCATC 1 cut(s) 204
SfcI CTRYAG 1 cut(s) 18
Sse9I AATT 2 cut(s) 50, 428
SsiI CCGC 2 cut(s) 85, 150
SspMI CTAG 1 cut(s) 276
StyD4I CCNGG 1 cut(s) 74
TaaI ACNGT 2 cut(s) 101, 286
TaqI TCGA 5 cut(s) 45, 109, 127, 157, 420
TasI AATT 2 cut(s) 50, 428
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 2 cut(s) 53, 393
Tru9I TTAA 2 cut(s) 53, 393
TscAI CASTG 3 cut(s) 205, 247, 289
TseFI GTSAC 2 cut(s) 133, 367
Tsp45I GTSAC 2 cut(s) 133, 367
TspDTI ATGAA 4 cut(s) 17, 284, 307, 441
TspRI CASTG 3 cut(s) 205, 247, 289
XceI RCATGY 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 109
XspI CTAG 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.