RchiOBHm_Chr2g0137351

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017339
Physical Location & Seq
Forward (+)
55070996 .. 55072453
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGGGTAAGGAAGTGTTTGATACATTCTTTTTGATTCACGGAGCACCGCTGGTGCTGATCGACGACACATTACCCGCCAGAACTTTCTTGAAGTCGTGGAGGGAGAAGATCTTGCCGGAGAAGCCCAAATATGTCCTCGTGATTTCGGGTCACTGGGAGACCAACGAACCCTCCATCAATGTCGTCGACCGCCATGAAACAATCCACGATTTCGAAGACTTCCCGCCCGAGATGTACGAGGTACCTCCAGTACCCAGCTCCGGGATCTCAAGAACTGGCCACCAAGGTGAACGACGTCCTAGTGGCCGCCGGGTACAAGCCTCTCCACGTGGACACAAAGCGAGGCCTCGACCACGGAGCCTGGGTCCCACTCATGCAAATGTACCCCGAAGCCGACGTGCCGGTGTGCCAGCTCTCCATCCAGTCCCACGAGAACGGAACCTATCAGTCTATCACTTCAACTTGGGCAAGGCCCTGGCTCCTCTCAGGGAGCAAGGAGTTCTCATCATCGGCACCGGAAGCGTCACCCACAACATGGAGTCCCTCATCATCAACAACGTCGGCGGCACAGTGGCCGACTGGGCTCTCGAGTTCGACGACTATGTCAAGGACGCGCTGCTCAACGGAAGGTACGATGAGGTGAACCAGGCTTTGGAGAAACATCCGCACGCCAAGATGGCTCACCCGGATGGACCAGTGCATTTCTATCCCCTACTGGCGGCCATCGGGGCCGCCGGAGGGGAAAAAGCCAAGGCGGAGCTCATTCATCATAGCTTTAGCCGGCATACTATTTCCTATGCCAACTACCGGTTTAAGTCAGCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

275

Amino Acids

30.72

Weight (kDa)

9.02

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 8 - 78 4.1e-06 Catalytic LigB subunit of aromatic ring-opening dioxygenase
LigB PF02900 151 - 271 2.1e-17 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017339)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30880
malus_domestica MD05G1186100.v1.1
rosa_chinensis RchiOBHm_Chr2g0137351
rosa_laevigata RLG00000019652
rosa_multiflora Rmu_sc0003208.1_g000024
rosa_roxburghii Rroxscaffold_2G00105650 Rroxscaffold_2G00107130
rosa_rugosa Rorug02G0341300
rosa_samantha Rh2AG389800 Rh2BG397300 Rh2CG377300 Rh2DG412500
rosa_wichuraiana Rw2G031930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 602
AatII GACGTC 1 cut(s) 298
Acc65I GGTACC 1 cut(s) 241
AccB1I GGYRCC 2 cut(s) 241, 512
AccB7I CCANNNNNTGG 2 cut(s) 535, 652
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 614
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 4 cut(s) 277, 304, 573, 720
AcvI CACGTG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 295
AfaI GTAC 6 cut(s) 236, 243, 252, 315, 384, 632
AfiI CCNNNNNNNGG 7 cut(s) 260, 261, 535, 652, 718, 738, 807
AgeI ACCGGT 1 cut(s) 807
AgsI TTSAA 2 cut(s) 91, 460
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 3 cut(s) 360, 474, 645
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AleI CACNNNNGTG 1 cut(s) 285
AluBI AGCT 5 cut(s) 258, 413, 760, 774, 821
AluI AGCT 5 cut(s) 258, 413, 760, 774, 821
Alw21I GWGCWC 2 cut(s) 46, 762
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 272
Ama87I CYCGRG 2 cut(s) 227, 587
AoxI GGCC 7 cut(s) 277, 304, 344, 471, 573, 720, 729
ApeKI GCWGC 1 cut(s) 616
AsiGI ACCGGT 1 cut(s) 807
Asp718I GGTACC 1 cut(s) 241
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 4 cut(s) 365, 472, 692, 729
AsuC2I CCSGG 3 cut(s) 262, 311, 686
AsuHPI GGTGA 4 cut(s) 299, 517, 652, 674
AsuII TTCGAA 1 cut(s) 213
AvaI CYCGRG 2 cut(s) 227, 587
AvaII GGWCC 2 cut(s) 365, 692
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 2 cut(s) 241, 512
BanII GRGCYC 2 cut(s) 586, 762
BauI CACGAG 2 cut(s) 137, 429
BbrPI CACGTG 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 2 cut(s) 46, 762
BbvI GCAGC 1 cut(s) 603
BccI CCATC 5 cut(s) 182, 426, 670, 683, 731
BciT130I CCWGG 3 cut(s) 362, 476, 647
BcnI CCSGG 3 cut(s) 262, 311, 686
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 300
BglI GCCNNNNNGGC 3 cut(s) 581, 677, 728
BglII AGATCT 1 cut(s) 108
BisI GCNGC 5 cut(s) 307, 565, 617, 720, 732
BlsI GCNGC 5 cut(s) 308, 566, 618, 721, 733
Bme1390I CCNGG 6 cut(s) 262, 311, 362, 476, 647, 686
Bme18I GGWCC 2 cut(s) 365, 692
BmeT110I CYCGRG 2 cut(s) 227, 587
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 4 cut(s) 365, 472, 692, 729
BmiI GGNNCC 8 cut(s) 243, 359, 366, 367, 440, 480, 514, 730
BmrFI CCNGG 6 cut(s) 262, 311, 362, 476, 647, 686
BmrI ACTGGG 2 cut(s) 163, 589
BmuI ACTGGG 2 cut(s) 163, 589
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 231
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 253
BpuMI CCSGG 3 cut(s) 262, 311, 686
BsaAI YACGTR 1 cut(s) 329
BsaHI GRCGYC 1 cut(s) 295
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 5 cut(s) 283, 353, 361, 474, 750
BsaWI WCCGGW 2 cut(s) 515, 807
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 349, 379
Bsc4I CCNNNNNNNGG 7 cut(s) 260, 261, 535, 652, 718, 738, 807
Bse118I RCCGGY 3 cut(s) 401, 780, 807
Bse1I ACTGG 7 cut(s) 158, 248, 280, 422, 584, 695, 720
BseBI CCWGG 3 cut(s) 362, 476, 647
BseDI CCNNGG 5 cut(s) 283, 353, 361, 474, 750
BseGI GGATG 3 cut(s) 418, 661, 694
BseLI CCNNNNNNNGG 7 cut(s) 260, 261, 535, 652, 718, 738, 807
BseMII CTCAG 2 cut(s) 499, 813
BseNI ACTGG 7 cut(s) 158, 248, 280, 422, 584, 695, 720
BseRI GAGGAG 1 cut(s) 471
BseXI GCAGC 1 cut(s) 603
BseYI CCCAGC 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 614
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 7 cut(s) 279, 306, 346, 473, 575, 722, 731
BshNI GGYRCC 2 cut(s) 241, 512
BshTI ACCGGT 1 cut(s) 807
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 2 cut(s) 46, 762
BsiHKCI CYCGRG 2 cut(s) 227, 587
BsiSI CCGG 9 cut(s) 116, 261, 310, 402, 516, 686, 735, 781, 808
BslFI GGGAC 3 cut(s) 351, 410, 526
BslI CCNNNNNNNGG 7 cut(s) 260, 261, 535, 652, 718, 738, 807
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 3 cut(s) 351, 410, 526
BsnI GGCC 7 cut(s) 279, 306, 346, 473, 575, 722, 731
Bso31I GGTCTC 1 cut(s) 152
BsoBI CYCGRG 2 cut(s) 227, 587
Bsp119I TTCGAA 1 cut(s) 213
Bsp1286I GDGCHC 3 cut(s) 46, 586, 762
Bsp143I GATC 3 cut(s) 57, 108, 264
BspANI GGCC 7 cut(s) 279, 306, 346, 473, 575, 722, 731
BspCNI CTCAG 2 cut(s) 498, 814
BspFNI CGCG 1 cut(s) 614
BspLI GGNNCC 8 cut(s) 243, 359, 366, 367, 440, 480, 514, 730
BspPI GGATC 1 cut(s) 272
BspT104I TTCGAA 1 cut(s) 213
BspT107I GGYRCC 2 cut(s) 241, 512
BspTNI GGTCTC 1 cut(s) 152
BsrFI RCCGGY 3 cut(s) 401, 780, 807
BsrI ACTGG 7 cut(s) 158, 248, 280, 422, 584, 695, 720
BssAI RCCGGY 3 cut(s) 401, 780, 807
BssECI CCNNGG 5 cut(s) 283, 353, 361, 474, 750
BssMI GATC 3 cut(s) 57, 108, 264
BssNI GRCGYC 1 cut(s) 295
BssSI CACGAG 2 cut(s) 137, 429
BssT1I CCWWGG 2 cut(s) 283, 750
Bst2BI CACGAG 2 cut(s) 137, 429
Bst2UI CCWGG 3 cut(s) 362, 476, 647
Bst4CI ACNGT 1 cut(s) 571
BstACI GRCGYC 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 411, 669, 782
BstDEI CTNAG 2 cut(s) 485, 822
BstDSI CCRYGG 1 cut(s) 353
BstF5I GGATG 3 cut(s) 418, 661, 694
BstFNI CGCG 1 cut(s) 614
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 3 cut(s) 60, 111, 267
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 3 cut(s) 57, 108, 264
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 5 cut(s) 121, 519, 581, 677, 728
BstNI CCWGG 3 cut(s) 362, 476, 647
BstSCI CCNGG 6 cut(s) 260, 309, 360, 474, 645, 684
BstUI CGCG 1 cut(s) 614
BstV1I GCAGC 1 cut(s) 603
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 2 cut(s) 108, 264
BstYI RGATCY 2 cut(s) 108, 264
BsuRI GGCC 7 cut(s) 279, 306, 346, 473, 575, 722, 731
BtgI CCRYGG 1 cut(s) 353
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 3 cut(s) 418, 661, 694
BtsIMutI CAGTG 3 cut(s) 151, 576, 702
Cac8I GCNNGC 3 cut(s) 411, 669, 782
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 3 cut(s) 401, 780, 807
Cfr13I GGNCC 4 cut(s) 365, 472, 692, 729
CseI GACGC 2 cut(s) 511, 620
Csp6I GTAC 6 cut(s) 235, 242, 251, 314, 383, 631
CspAI ACCGGT 1 cut(s) 807
CviAII CATG 3 cut(s) 194, 374, 535
CviQI GTAC 6 cut(s) 235, 242, 251, 314, 383, 631
DdeI CTNAG 2 cut(s) 485, 822
DpnI GATC 3 cut(s) 59, 110, 266
DpnII GATC 3 cut(s) 57, 108, 264
DrdI GACNNNNNNGTC 1 cut(s) 602
DseDI GACNNNNNNGTC 1 cut(s) 602
EaeI YGGCCR 4 cut(s) 277, 304, 573, 720
EciI GGCGGA 1 cut(s) 770
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 2 cut(s) 283, 750
Eco147I AGGCCT 1 cut(s) 346
Eco24I GRGCYC 2 cut(s) 586, 762
Eco31I GGTCTC 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 365, 692
Eco53kI GAGCTC 1 cut(s) 760
Eco72I CACGTG 1 cut(s) 329
Eco88I CYCGRG 2 cut(s) 227, 587
EcoICRI GAGCTC 1 cut(s) 760
EcoO109I RGGNCCY 2 cut(s) 365, 472
EcoRII CCWGG 3 cut(s) 360, 474, 645
EcoT14I CCWWGG 2 cut(s) 283, 750
EcoT38I GRGCYC 2 cut(s) 586, 762
ErhI CCWWGG 2 cut(s) 283, 750
FaeI CATG 3 cut(s) 197, 377, 538
FaiI YATR 8 cut(s) 132, 195, 375, 536, 603, 771, 786, 798
FaqI GGGAC 3 cut(s) 351, 410, 526
FatI CATG 3 cut(s) 193, 373, 534
FauI CCCGC 2 cut(s) 82, 231
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 307, 565, 617, 720, 732
FokI GGATG 3 cut(s) 405, 648, 701
FriOI GRGCYC 2 cut(s) 586, 762
Fsp4HI GCNGC 5 cut(s) 307, 565, 617, 720, 732
FspBI CTAG 1 cut(s) 300
GlaI GCGC 1 cut(s) 615
GluI GCNGC 5 cut(s) 307, 565, 617, 720, 732
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 7 cut(s) 279, 306, 346, 473, 575, 722, 731
HapII CCGG 9 cut(s) 116, 261, 310, 402, 516, 686, 735, 781, 808
HgaI GACGC 2 cut(s) 511, 620
HhaI GCGC 1 cut(s) 616
Hin1I GRCGYC 1 cut(s) 295
Hin1II CATG 3 cut(s) 197, 377, 538
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 2 cut(s) 34, 539
HpaII CCGG 9 cut(s) 116, 261, 310, 402, 516, 686, 735, 781, 808
HphI GGTGA 4 cut(s) 299, 517, 652, 674
Hpy166II GTNNAC 4 cut(s) 187, 290, 332, 643
Hpy188III TCNNGA 4 cut(s) 88, 139, 270, 587
Hpy8I GTNNAC 4 cut(s) 187, 290, 332, 643
Hpy99I CGWCG 6 cut(s) 65, 188, 297, 399, 563, 599
HpyAV CCTTC 1 cut(s) 621
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4IV ACGT 4 cut(s) 295, 328, 397, 558
HpyCH4V TGCA 2 cut(s) 377, 700
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 519, 581, 677, 728
HpyF3I CTNAG 2 cut(s) 485, 822
HpySE526I ACGT 4 cut(s) 295, 328, 397, 558
Hsp92I GRCGYC 1 cut(s) 295
Hsp92II CATG 3 cut(s) 197, 377, 538
HspAI GCGC 1 cut(s) 614
KflI GGGWCCC 1 cut(s) 365
KpnI GGTACC 1 cut(s) 245
KroI GCCGGC 1 cut(s) 780
KroNI GCCGGC 1 cut(s) 782
Kzo9I GATC 3 cut(s) 57, 108, 264
LmnI GCTCC 6 cut(s) 41, 263, 357, 484, 490, 757
Lsp1109I GCAGC 1 cut(s) 603
MaeI CTAG 1 cut(s) 300
MaeII ACGT 4 cut(s) 295, 328, 397, 558
MaeIII GTNAC 2 cut(s) 149, 523
MalI GATC 3 cut(s) 59, 110, 266
MboI GATC 3 cut(s) 57, 108, 264
MboII GAAGA 2 cut(s) 118, 227
MflI RGATCY 2 cut(s) 108, 264
MhlI GDGCHC 3 cut(s) 46, 586, 762
MlsI TGGCCA 1 cut(s) 279
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 1 cut(s) 548
Mox20I TGGCCA 1 cut(s) 279
MroNI GCCGGC 1 cut(s) 780
MscI TGGCCA 1 cut(s) 279
MseI TTAA 1 cut(s) 813
MslI CAYNNNNRTG 3 cut(s) 285, 378, 687
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 49, 821
MspI CCGG 9 cut(s) 116, 261, 310, 402, 516, 686, 735, 781, 808
MspR9I CCNGG 6 cut(s) 262, 311, 362, 476, 647, 686
MvaI CCWGG 3 cut(s) 362, 476, 647
MvnI CGCG 1 cut(s) 614
MwoI GCNNNNNNNGC 5 cut(s) 121, 519, 581, 677, 728
NaeI GCCGGC 1 cut(s) 782
NciI CCSGG 3 cut(s) 262, 311, 686
NdeII GATC 3 cut(s) 57, 108, 264
NgoMIV GCCGGC 1 cut(s) 780
NlaIII CATG 3 cut(s) 197, 377, 538
NlaIV GGNNCC 8 cut(s) 243, 359, 366, 367, 440, 480, 514, 730
NmuCI GTSAC 2 cut(s) 149, 523
NspV TTCGAA 1 cut(s) 213
OliI CACNNNNGTG 1 cut(s) 285
PaeR7I CTCGAG 1 cut(s) 587
PceI AGGCCT 1 cut(s) 346
PcsI WCGNNNNNNNCGW 2 cut(s) 594, 630
PdiI GCCGGC 1 cut(s) 782
PfeI GAWTC 1 cut(s) 34
PflFI GACNNNGTC 1 cut(s) 602
PflMI CCANNNNNTGG 2 cut(s) 535, 652
PfoI TCCNGGA 1 cut(s) 260
PinAI ACCGGT 1 cut(s) 807
PkrI GCNGC 5 cut(s) 308, 566, 618, 721, 733
PleI GAGTC 1 cut(s) 547
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 1 cut(s) 547
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 365
Psp124BI GAGCTC 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 365
Psp6I CCWGG 3 cut(s) 360, 474, 645
PspCI CACGTG 1 cut(s) 329
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 3 cut(s) 360, 474, 645
PspN4I GGNNCC 8 cut(s) 243, 359, 366, 367, 440, 480, 514, 730
PspPI GGNCC 4 cut(s) 365, 472, 692, 729
PspPPI RGGWCCY 1 cut(s) 365
PsuI RGATCY 2 cut(s) 108, 264
PsyI GACNNNGTC 1 cut(s) 602
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 6 cut(s) 236, 243, 252, 315, 384, 632
RsaNI GTAC 6 cut(s) 235, 242, 251, 314, 383, 631
RseI CAYNNNNRTG 3 cut(s) 285, 378, 687
SacI GAGCTC 1 cut(s) 762
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 5 cut(s) 307, 565, 617, 720, 732
Sau3AI GATC 3 cut(s) 57, 108, 264
Sau96I GGNCC 4 cut(s) 365, 472, 692, 729
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 6 cut(s) 262, 311, 362, 476, 647, 686
SduI GDGCHC 3 cut(s) 46, 586, 762
SfiI GGCCNNNNNGGCC 1 cut(s) 728
Sfr274I CTCGAG 1 cut(s) 587
SfuI TTCGAA 1 cut(s) 213
SinI GGWCC 2 cut(s) 365, 692
SlaI CTCGAG 1 cut(s) 587
SmiMI CAYNNNNRTG 3 cut(s) 285, 378, 687
SmlI CTYRAG 2 cut(s) 268, 587
SmoI CTYRAG 2 cut(s) 268, 587
SseBI AGGCCT 1 cut(s) 346
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 1 cut(s) 762
StuI AGGCCT 1 cut(s) 346
StyD4I CCNGG 6 cut(s) 260, 309, 360, 474, 645, 684
StyI CCWWGG 2 cut(s) 283, 750
TaaI ACNGT 1 cut(s) 571
TaiI ACGT 4 cut(s) 298, 331, 400, 561
TaqI TCGA 6 cut(s) 60, 186, 213, 349, 588, 594
TauI GCSGC 4 cut(s) 309, 567, 722, 734
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TscAI CASTG 3 cut(s) 158, 576, 702
TseFI GTSAC 2 cut(s) 149, 523
TseI GCWGC 1 cut(s) 616
Tsp45I GTSAC 2 cut(s) 149, 523
TspDTI ATGAA 2 cut(s) 210, 755
TspGWI ACGGA 4 cut(s) 54, 370, 451, 639
TspRI CASTG 3 cut(s) 158, 576, 702
Tth111I GACNNNGTC 1 cut(s) 602
Van91I CCANNNNNTGG 2 cut(s) 535, 652
VpaK11BI GGWCC 2 cut(s) 365, 692
XhoI CTCGAG 1 cut(s) 587
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 300
ZraI GACGTC 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.