RchiOBHm_Chr2g0143451

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010424
Physical Location & Seq
Reverse (-)
61087748 .. 61094309
6562 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2163 bp
ATGAAGTATAGTAAGAGCTTTCACCATCACCATAATAGTTCAGGAGTGTCGAGCTCCAACTGGTCAACAATGGCCAGGAAGAGAAGCACAAGACTCACCTTGTTCTTCTTCCTCCTCCTCCTCTCTCTCATAGCCTTCGGGGTCTTTGTCCCTGTCTTTGCTCTTCTACCTTCTCTTTCCCAATCTCAAAATGACCAGCAGCACCATCCAACAACCAAAAGTAGTCCAGATTATGTCAGAAAGTTTGAAATTTCAGAAGACAGGTTCTGGAAAGATGGTCAGCCTTTCCAGATCATTGGGGGTGACTTGCATTATTTTCGGGTTCTTCCTGAGTACTGGGAAGATAGGCTGTTGAGAGCAAAGGCGCTGGGATTAAATACTATTCAAACTTATGTACCCTGGAACCTGCATGAACCAAGAGCTGGTACTTCGAATTTTGCGGGTATTGCAGATTTAGTGTCATTTCTCGAACTCTGCCAGAAGCTTGGGTTTGCTGTTATGCTTAGACCTGGGCCATATATATGTGGAGAGTGGGATTTGGGTGGTTTCCCTGCTTGGTTACTTGCCATCAAGCCAGCTCTCACACTAAGATCATCTGACCCAGCTTTCCTTCAATTGGTTGAAAGATGGTGGGGAAGCCTACTTCCAAAGATAGCTCCTCTTCTTTATGGAAATGGTGGTCCAATTATAATGGTTCAGATTGAAAATGAGTATGGTTCATATGCAAGTGACAAAGCTTATCTTCATCACTTGGTCTCACTGGCTAGAAAGCACCTTGGGGATGAAGTAATTTTGTATACTACAGATGGAGGTTCTAGGGAAACTCTGGAGAAAGGAACAATTCGTGGAGATGCTGTCTTTTCTGCTGTTGACTTCACTACTGGTGATAATCCTTGGCCTATATTTGAGTTACAAAAAGAGTTCAATGCCGCAGGGAAATCACCGCCACTTTCTTCGGAGTTTTACACAGGTTGGCTAACACACTGGGGGGAGCAGAATGCGCAAACTGACGCTGATTTTACAGCAGCTTCCTTAAAAAGAATTTTAGAAAGAAATGGTTCTGCAGTGCTTTATATGGCACATGGAGGAACAAACTTTGGATTTTATAATGGAGCAAACACTGGTTCAGATGAGTCTGATTACAAGCCTGATCTCACTTCCTATGATTATGATGCACCAATTAGGGAGTCTGGGGATGTGAGCAATGCAAAATTCAAAGCACTTAGGAAGGTTATAGAGCAATACAGTTCAGAATCTCTCCCTCCAATTCCATCCGATATTGAAAAGAAAACATACGGATCTATTCACGTATCAAAAACCGGGAATTTGTTTGATATGATTCATGATTTTGATGTGGTTGAATCTGAAAACCCAATAACAATGGAGTCGGTAGGCCAGATGTCTGGATTTTTGTTATATGTAACTGAATATGCTGCAAAGGACTATGGTGGTGGAAACATTGTATCCATACCAAAGGTGCATGACAGAGCTCAAGTTTTTATATCATGTCCTTCTCAAGATGGTCATGGAAAACCAACATACGTTGGCACATTTGAAAGATGGTCGAATCAACCACTGTCCCTTCCTAATACTAAATGTCTCTCCAAAGTCAGCTTATATGTGCTGGTTGAAAATATGGGACGTTTAAATTATGGGCCATATATCTTTGACATGAAGGGTATTCTATCTTCTGTTTATCTGGATGGAAGAATTCTCCGTGGATGGAAAATGTACTTGTTGCCTCTAATCAACCTGAACGAGAATCCGAAAGTCAATCCCATAATTCAGGCTACAGATTCTGGATTCATTACAATGTCAACCCATAAAAAGTTAAAACCGAAGTCTGCAACTGCTTCAAATGAACCAGCATTCTATGCTGGAAAGTTTTCTATCAGCAAACAAGACAAAATAAAAGATACGTTCATATCATTGAGTGGTTGGGGTAAAGGAATTGTGACTATTAACAATTTCAATATAGGAAGATTTTGGCCGTTAAAGGGACCACAGTGCAACCTTTATGTTCCTGCTCCTATCCTTAAGCATGGGGAGAACATTGTGGTGATATTTGAGTTACAAGCGCCAAACCCTGATCTTGTGGTACACTTGGTCGATCAGCCAGACTTTACCTGTGGTCCAAGTAAATCGAATCTGCATCAATTGTAG

Protein Analysis

720

Amino Acids

80.44

Weight (kDa)

6.73

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 89 - 414 2.8e-118 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 108 - 242 4.6e-06 Beta-galactosidase
BetaGal_ABD_1 PF21317 458 - 580 6.6e-23 Beta-galactosidase, first all-beta domain
BetaGal_gal-bd PF21467 622 - 684 4e-22 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72990 AT1G72990 AT1G72990 AT1G72990
fragaria_vesca FvH4_6g33540 FvH4_6g33540 FvH4_6g33540
malus_domestica MD09G1192500.v1.1
prunus_persica Prupe.3G048800_v2.0.a1 Prupe.3G048800_v2.0.a1
pyrus_communis pycom09g10910
rosa_chinensis RchiOBHm_Chr2g0143451
rosa_laevigata RLG00000020036
rosa_multiflora Rmu_sc0003507.1_g000021
rosa_roxburghii Rroxscaffold_2G00101830
rosa_rugosa Rorug02G0381500
rosa_samantha Rh2AG434500 Rh2BG442300 Rh2CG420400 Rh2DG452500
rosa_wichuraiana Rw2G035420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 689, 1107
Acc16I TGCGCA 1 cut(s) 1002
Acc36I ACCTGC 1 cut(s) 414
AccB7I CCANNNNNTGG 1 cut(s) 422
AccI GTMKAC 1 cut(s) 797
AciI CCGC 3 cut(s) 440, 930, 944
AclWI GGATC 1 cut(s) 1306
AcoI YGGCCR 2 cut(s) 72, 1988
AcsI RAATTY 6 cut(s) 249, 433, 1041, 1211, 1324, 1710
AfaI GTAC 5 cut(s) 335, 396, 427, 1733, 2100
AfiI CCNNNNNNNGG 3 cut(s) 422, 616, 1997
AflII CTTAAG 1 cut(s) 2036
AjnI CCWGG 3 cut(s) 74, 398, 508
Alw21I GWGCWC 2 cut(s) 56, 1492
Alw26I GTCTC 2 cut(s) 760, 1604
AlwI GGATC 1 cut(s) 1306
AlwNI CAGNNNCTG 2 cut(s) 267, 1799
AoxI GGCC 6 cut(s) 72, 512, 896, 1393, 1655, 1988
ApeKI GCWGC 3 cut(s) 199, 1025, 1433
ApoI RAATTY 6 cut(s) 249, 433, 1041, 1211, 1324, 1710
Asp700I GAANNNNTTC 2 cut(s) 1057, 1885
AspLEI GCGC 3 cut(s) 367, 1003, 2080
AspS9I GGNCC 5 cut(s) 512, 680, 1655, 2000, 2132
AsuC2I CCSGG 1 cut(s) 1321
AsuHPI GGTGA 7 cut(s) 14, 20, 88, 314, 896, 933, 2071
AsuII TTCGAA 1 cut(s) 431
AvaII GGWCC 3 cut(s) 680, 2000, 2132
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BalI TGGCCA 1 cut(s) 74
BanII GRGCYC 2 cut(s) 56, 1492
BarI GAAGNNNNNNTAC 2 cut(s) 1672, 1704
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 2 cut(s) 56, 1492
BbvI GCAGC 3 cut(s) 211, 1037, 1420
BceAI ACGGC 1 cut(s) 1975
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BciT130I CCWGG 3 cut(s) 76, 400, 510
BciVI GTATCC 1 cut(s) 1474
BcnI CCSGG 1 cut(s) 1321
BcoDI GTCTC 2 cut(s) 760, 1604
BfaI CTAG 2 cut(s) 765, 816
BfmI CTRYAG 3 cut(s) 801, 1062, 1791
BfoI RGCGCY 2 cut(s) 368, 2081
BfrI CTTAAG 1 cut(s) 2036
BfuAI ACCTGC 1 cut(s) 414
BfuI GTATCC 1 cut(s) 1474
BisI GCNGC 4 cut(s) 200, 930, 1026, 1434
BlsI GCNGC 4 cut(s) 201, 931, 1027, 1435
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 4 cut(s) 76, 400, 510, 1321
Bme18I GGWCC 3 cut(s) 680, 2000, 2132
BmgT120I GGNCC 5 cut(s) 512, 680, 1655, 2000, 2132
BmiI GGNNCC 2 cut(s) 404, 2001
BmrFI CCNGG 4 cut(s) 76, 400, 510, 1321
BmrI ACTGGG 2 cut(s) 346, 994
BmsI GCATC 2 cut(s) 841, 1162
BmuI ACTGGG 2 cut(s) 346, 994
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 848
Bpu14I TTCGAA 1 cut(s) 431
BpuEI CTTGAG 2 cut(s) 1476, 1500
BpuMI CCSGG 1 cut(s) 1321
BsaAI YACGTR 1 cut(s) 1309
BsaI GGTCTC 1 cut(s) 760
BsaJI CCNNGG 5 cut(s) 398, 509, 775, 893, 1717
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bsc4I CCNNNNNNNGG 3 cut(s) 422, 616, 1997
Bse1I ACTGG 6 cut(s) 65, 341, 765, 886, 989, 1126
Bse3DI GCAATG 1 cut(s) 1210
BseBI CCWGG 3 cut(s) 76, 400, 510
BseDI CCNNGG 5 cut(s) 398, 509, 775, 893, 1717
BseGI GGATG 6 cut(s) 205, 787, 1201, 1271, 1708, 1727
BseLI CCNNNNNNNGG 3 cut(s) 422, 616, 1997
BseMI GCAATG 1 cut(s) 1210
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 6 cut(s) 65, 341, 765, 886, 989, 1126
BseRI GAGGAG 4 cut(s) 104, 107, 110, 648
BseXI GCAGC 3 cut(s) 211, 1037, 1420
BseYI CCCAGC 2 cut(s) 367, 601
BshFI GGCC 6 cut(s) 74, 514, 898, 1395, 1657, 1990
BsiHKAI GWGCWC 2 cut(s) 56, 1492
BsiSI CCGG 1 cut(s) 1320
BslFI GGGAC 4 cut(s) 134, 1564, 1653, 2013
BslI CCNNNNNNNGG 3 cut(s) 422, 616, 1997
BsmAI GTCTC 2 cut(s) 760, 1604
BsmFI GGGAC 4 cut(s) 134, 1564, 1653, 2013
BsmI GAATGC 2 cut(s) 1003, 1868
BsnI GGCC 6 cut(s) 74, 514, 898, 1395, 1657, 1990
Bso31I GGTCTC 1 cut(s) 760
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 56, 1492
Bsp143I GATC 6 cut(s) 291, 590, 1150, 1298, 2089, 2110
BspACI CCGC 3 cut(s) 440, 930, 944
BspANI GGCC 6 cut(s) 74, 514, 898, 1395, 1657, 1990
BspCNI CTCAG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 1342
BspLI GGNNCC 2 cut(s) 404, 2001
BspMAI CTGCAG 1 cut(s) 1066
BspMI ACCTGC 1 cut(s) 414
BspPI GGATC 1 cut(s) 1306
BspQI GCTCTTC 1 cut(s) 168
BspT104I TTCGAA 1 cut(s) 431
BspTI CTTAAG 1 cut(s) 2036
BspTNI GGTCTC 1 cut(s) 760
BsrDI GCAATG 1 cut(s) 1210
BsrI ACTGG 6 cut(s) 65, 341, 765, 886, 989, 1126
BssECI CCNNGG 5 cut(s) 398, 509, 775, 893, 1717
BssMI GATC 6 cut(s) 291, 590, 1150, 1298, 2089, 2110
BssNAI GTATAC 1 cut(s) 798
BssT1I CCWWGG 2 cut(s) 775, 893
Bst1107I GTATAC 1 cut(s) 798
Bst2UI CCWGG 3 cut(s) 76, 400, 510
Bst4CI ACNGT 3 cut(s) 1247, 1578, 2007
Bst6I CTCTTC 3 cut(s) 74, 168, 666
BstAFI CTTAAG 1 cut(s) 2036
BstAPI GCANNNNNTGC 1 cut(s) 1874
BstBAI YACGTR 1 cut(s) 1309
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 4 cut(s) 330, 503, 587, 1223
BstDSI CCRYGG 1 cut(s) 1717
BstF5I GGATG 6 cut(s) 205, 787, 1201, 1271, 1708, 1727
BstH2I RGCGCY 2 cut(s) 368, 2081
BstHHI GCGC 3 cut(s) 367, 1003, 2080
BstKTI GATC 6 cut(s) 294, 593, 1153, 1301, 2092, 2113
BstMAI GTCTC 2 cut(s) 760, 1604
BstMBI GATC 6 cut(s) 291, 590, 1150, 1298, 2089, 2110
BstMWI GCNNNNNNNGC 3 cut(s) 446, 1000, 1874
BstNI CCWGG 3 cut(s) 76, 400, 510
BstSCI CCNGG 4 cut(s) 74, 398, 508, 1319
BstSFI CTRYAG 3 cut(s) 801, 1062, 1791
BstV1I GCAGC 3 cut(s) 211, 1037, 1420
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 1298
BstXI CCANNNNNNTGG 3 cut(s) 296, 485, 1403
BstYI RGATCY 1 cut(s) 1298
BstZ17I GTATAC 1 cut(s) 798
BsuI GTATCC 1 cut(s) 1474
BsuRI GGCC 6 cut(s) 74, 514, 898, 1395, 1657, 1990
BtgI CCRYGG 1 cut(s) 1717
BtsCI GGATG 6 cut(s) 205, 787, 1201, 1271, 1708, 1727
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 6 cut(s) 758, 982, 1071, 1119, 1574, 2012
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 576
CaiI CAGNNNCTG 2 cut(s) 267, 1799
CciI TCATGA 1 cut(s) 1342
CfoI GCGC 3 cut(s) 367, 1003, 2080
Cfr13I GGNCC 5 cut(s) 512, 680, 1655, 2000, 2132
CseI GACGC 1 cut(s) 1019
Csp6I GTAC 5 cut(s) 334, 395, 426, 1732, 2099
CviAII CATG 8 cut(s) 410, 1082, 1343, 1481, 1506, 1526, 1672, 2042
CviQI GTAC 5 cut(s) 334, 395, 426, 1732, 2099
DdeI CTNAG 4 cut(s) 330, 503, 587, 1223
DpnI GATC 6 cut(s) 293, 592, 1152, 1300, 2091, 2112
DpnII GATC 6 cut(s) 291, 590, 1150, 1298, 2089, 2110
DraI TTTAAA 1 cut(s) 1647
EaeI YGGCCR 2 cut(s) 72, 1988
Eam1104I CTCTTC 3 cut(s) 74, 168, 666
EarI CTCTTC 3 cut(s) 74, 168, 666
Ecl136II GAGCTC 2 cut(s) 54, 1490
Eco130I CCWWGG 2 cut(s) 775, 893
Eco24I GRGCYC 2 cut(s) 56, 1492
Eco31I GGTCTC 1 cut(s) 760
Eco47I GGWCC 3 cut(s) 680, 2000, 2132
Eco53kI GAGCTC 2 cut(s) 54, 1490
EcoICRI GAGCTC 2 cut(s) 54, 1490
EcoRI GAATTC 1 cut(s) 1710
EcoRII CCWGG 3 cut(s) 74, 398, 508
EcoT14I CCWWGG 2 cut(s) 775, 893
EcoT38I GRGCYC 2 cut(s) 56, 1492
ErhI CCWWGG 2 cut(s) 775, 893
FaeI CATG 8 cut(s) 413, 1085, 1346, 1484, 1509, 1529, 1675, 2045
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 4 cut(s) 134, 1564, 1653, 2013
FatI CATG 8 cut(s) 409, 1081, 1342, 1480, 1505, 1525, 1671, 2041
FauI CCCGC 1 cut(s) 433
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 1 cut(s) 797
Fnu4HI GCNGC 4 cut(s) 200, 930, 1026, 1434
FokI GGATG 6 cut(s) 192, 794, 1208, 1258, 1715, 1734
FriOI GRGCYC 2 cut(s) 56, 1492
Fsp4HI GCNGC 4 cut(s) 200, 930, 1026, 1434
FspBI CTAG 2 cut(s) 765, 816
FspI TGCGCA 1 cut(s) 1002
GlaI GCGC 3 cut(s) 366, 1002, 2079
GluI GCNGC 4 cut(s) 200, 930, 1026, 1434
GsaI CCCAGC 2 cut(s) 371, 605
GsuI CTGGAG 1 cut(s) 848
HaeII RGCGCY 2 cut(s) 368, 2081
HaeIII GGCC 6 cut(s) 74, 514, 898, 1395, 1657, 1990
HapII CCGG 1 cut(s) 1320
HgaI GACGC 1 cut(s) 1019
HhaI GCGC 3 cut(s) 367, 1003, 2080
Hin1II CATG 8 cut(s) 413, 1085, 1346, 1484, 1509, 1529, 1675, 2045
Hin6I GCGC 3 cut(s) 365, 1001, 2078
HinP1I GCGC 3 cut(s) 365, 1001, 2078
HincII GTYRAC 3 cut(s) 66, 871, 1818
HindII GTYRAC 3 cut(s) 66, 871, 1818
HindIII AAGCTT 2 cut(s) 482, 735
HpaII CCGG 1 cut(s) 1320
HphI GGTGA 7 cut(s) 14, 20, 88, 314, 896, 933, 2071
Hpy166II GTNNAC 5 cut(s) 66, 798, 871, 1818, 2101
Hpy8I GTNNAC 5 cut(s) 66, 798, 871, 1818, 2101
HpyAV CCTTC 7 cut(s) 145, 180, 620, 1222, 1521, 1592, 1669
HpyCH4III ACNGT 3 cut(s) 1247, 1578, 2007
HpyCH4IV ACGT 4 cut(s) 1308, 1542, 1642, 1919
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 1000, 1874
HpyF3I CTNAG 4 cut(s) 330, 503, 587, 1223
HpySE526I ACGT 4 cut(s) 1308, 1542, 1642, 1919
Hsp92II CATG 8 cut(s) 413, 1085, 1346, 1484, 1509, 1529, 1675, 2045
HspAI GCGC 3 cut(s) 365, 1001, 2078
Kzo9I GATC 6 cut(s) 291, 590, 1150, 1298, 2089, 2110
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 5 cut(s) 59, 661, 991, 1112, 2032
Lsp1109I GCAGC 3 cut(s) 211, 1037, 1420
LweI GCATC 2 cut(s) 841, 1162
MaeI CTAG 2 cut(s) 765, 816
MaeII ACGT 4 cut(s) 1308, 1542, 1642, 1919
MaeIII GTNAC 7 cut(s) 302, 558, 728, 909, 1420, 1954, 2070
MalI GATC 6 cut(s) 293, 592, 1152, 1300, 2091, 2112
MboI GATC 6 cut(s) 291, 590, 1150, 1298, 2089, 2110
MfeI CAATTG 2 cut(s) 614, 2156
MflI RGATCY 1 cut(s) 1298
MhlI GDGCHC 2 cut(s) 56, 1492
MlsI TGGCCA 1 cut(s) 74
MluNI TGGCCA 1 cut(s) 74
MlyI GAGTC 4 cut(s) 87, 1142, 1196, 1394
MmeI TCCRAC 2 cut(s) 81, 233
MnlI CCTC 9 cut(s) 122, 125, 128, 131, 669, 803, 1079, 1272, 1752
Mox20I TGGCCA 1 cut(s) 74
MroXI GAANNNNTTC 2 cut(s) 1057, 1885
MscI TGGCCA 1 cut(s) 74
MseI TTAA 7 cut(s) 374, 1034, 1646, 1832, 1962, 1994, 2037
MslI CAYNNNNRTG 3 cut(s) 520, 1811, 2057
Msp20I TGGCCA 1 cut(s) 74
MspCI CTTAAG 1 cut(s) 2036
MspI CCGG 1 cut(s) 1320
MspR9I CCNGG 4 cut(s) 76, 400, 510, 1321
MunI CAATTG 2 cut(s) 614, 2156
Mva1269I GAATGC 2 cut(s) 1003, 1868
MvaI CCWGG 3 cut(s) 76, 400, 510
MwoI GCNNNNNNNGC 3 cut(s) 446, 1000, 1874
NciI CCSGG 1 cut(s) 1321
NdeI CATATG 1 cut(s) 721
NdeII GATC 6 cut(s) 291, 590, 1150, 1298, 2089, 2110
NlaIII CATG 8 cut(s) 413, 1085, 1346, 1484, 1509, 1529, 1675, 2045
NlaIV GGNNCC 2 cut(s) 404, 2001
NmuCI GTSAC 3 cut(s) 302, 728, 1954
NsbI TGCGCA 1 cut(s) 1002
NspV TTCGAA 1 cut(s) 431
PagI TCATGA 1 cut(s) 1342
PciSI GCTCTTC 1 cut(s) 168
PctI GAATGC 2 cut(s) 1003, 1868
PdmI GAANNNNTTC 2 cut(s) 1057, 1885
PfeI GAWTC 8 cut(s) 1253, 1339, 1361, 1567, 1762, 1796, 1803, 2146
PflMI CCANNNNNTGG 1 cut(s) 422
PkrI GCNGC 4 cut(s) 201, 931, 1027, 1435
PleI GAGTC 4 cut(s) 87, 1141, 1195, 1393
PpsI GAGTC 4 cut(s) 87, 1141, 1195, 1393
Ppu21I YACGTR 1 cut(s) 1309
PsiI TTATAA 2 cut(s) 689, 1107
Psp124BI GAGCTC 2 cut(s) 56, 1492
Psp6I CCWGG 3 cut(s) 74, 398, 508
PspFI CCCAGC 2 cut(s) 367, 601
PspGI CCWGG 3 cut(s) 74, 398, 508
PspN4I GGNNCC 2 cut(s) 404, 2001
PspPI GGNCC 5 cut(s) 512, 680, 1655, 2000, 2132
PstI CTGCAG 1 cut(s) 1066
PstNI CAGNNNCTG 2 cut(s) 267, 1799
PsuI RGATCY 1 cut(s) 1298
RsaI GTAC 5 cut(s) 335, 396, 427, 1733, 2100
RsaNI GTAC 5 cut(s) 334, 395, 426, 1732, 2099
RseI CAYNNNNRTG 3 cut(s) 520, 1811, 2057
SacI GAGCTC 2 cut(s) 56, 1492
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 7 cut(s) 374, 1034, 1646, 1832, 1962, 1994, 2037
SatI GCNGC 4 cut(s) 200, 930, 1026, 1434
Sau3AI GATC 6 cut(s) 291, 590, 1150, 1298, 2089, 2110
Sau96I GGNCC 5 cut(s) 512, 680, 1655, 2000, 2132
ScaI AGTACT 1 cut(s) 335
SchI GAGTC 4 cut(s) 87, 1142, 1196, 1394
ScrFI CCNGG 4 cut(s) 76, 400, 510, 1321
SduI GDGCHC 2 cut(s) 56, 1492
SfaNI GCATC 2 cut(s) 841, 1162
SfcI CTRYAG 3 cut(s) 801, 1062, 1791
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 3 cut(s) 680, 2000, 2132
SmiMI CAYNNNNRTG 3 cut(s) 520, 1811, 2057
SmlI CTYRAG 3 cut(s) 1491, 1515, 2036
SmoI CTYRAG 3 cut(s) 1491, 1515, 2036
SsiI CCGC 3 cut(s) 440, 930, 944
SspMI CTAG 2 cut(s) 765, 816
SstI GAGCTC 2 cut(s) 56, 1492
StyD4I CCNGG 4 cut(s) 74, 398, 508, 1319
StyI CCWWGG 2 cut(s) 775, 893
TaaI ACNGT 3 cut(s) 1247, 1578, 2007
TaiI ACGT 4 cut(s) 1311, 1545, 1645, 1922
TaqI TCGA 6 cut(s) 50, 431, 468, 1565, 2109, 2144
TatI WGTACW 2 cut(s) 333, 1731
TauI GCSGC 1 cut(s) 932
TfiI GAWTC 8 cut(s) 1253, 1339, 1361, 1567, 1762, 1796, 1803, 2146
Tru1I TTAA 7 cut(s) 374, 1034, 1646, 1832, 1962, 1994, 2037
Tru9I TTAA 7 cut(s) 374, 1034, 1646, 1832, 1962, 1994, 2037
TscAI CASTG 6 cut(s) 765, 989, 1071, 1126, 1581, 2012
TseFI GTSAC 3 cut(s) 302, 728, 1954
TseI GCWGC 3 cut(s) 199, 1025, 1433
Tsp45I GTSAC 3 cut(s) 302, 728, 1954
TspGWI ACGGA 2 cut(s) 1311, 1706
TspRI CASTG 6 cut(s) 765, 989, 1071, 1126, 1581, 2012
Van91I CCANNNNNTGG 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 2036
VpaK11BI GGWCC 3 cut(s) 680, 2000, 2132
XapI RAATTY 6 cut(s) 249, 433, 1041, 1211, 1324, 1710
XmiI GTMKAC 1 cut(s) 797
XmnI GAANNNNTTC 2 cut(s) 1057, 1885
XspI CTAG 2 cut(s) 765, 816
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.