Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0017342

Physical Location & Seq

Genomic Coordinates Reverse (-)

64238982 .. 64244637

Gene Structure 5656 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4542 bp

ATGGATGCAGACTCCTCATTCGAGCTGGAAAACTCTATTTCCGGCCACCTCCCACGTTACACCACCCCTGTGGCAGGCTCGTCGCATTATGACTCCCGCTCATTCACACCGGTTAGCTCCGCTCACTCGAGAACTCCCAGAAGGTCTCGCCTCCCCACTCCAACTACGCCTTTCGCCTCGGACAATGACACGTCGTGGCAAGGCGAGATCTCCTGGCAGTTTGAGCCGACGGGGTGGAACGAAACTCGCAACTTGGGCTCAGCTATGAGCCCATGGGCCACCGTGAGCACGTCCTCTCGCCAGGCCCAGCGTGCCCACCGCTCAGCAAATTACTACTACCTCTCCCGCACCACTGGCGGGTTTCGTACCTCCCCAAATCAATATTACAACGAGTACTCCAGCTATGGTGCTGTGACAGATGGGAGACTTGAACTTAAGAGCTACGTTGCTAGGGACAGTGAAAGCTCAACTTTTGGGAGAAGTAGTTACAATTATGGGGAGCAGAAGAAACCCTTTAGGGCAGTTACTCCAAGGTTGGAGGGTATTAAAGAAGCAGCTGGGAGTGGAAATTTCAGCGGCAGTGGCCCTTTGGCTAAGAAAGATTTGCTTGGTTTGATCGATTATCAAACAGCAGAAGATGATGATGAAGATGAGAGTTTGGGTCATAGTGGACACCATGGTCATGGCCTATATCTTGATCATGGTCATAGGCCATCACACAAAGGACATGATCATGGGTTGTCACCCATGGGTCATCATGGTCATGACACGTGGCCGCATTCAATTACTAGTCACGATATTGATGATGAGAGTGATCAGGGTTCTGGGTATGAGGATGAGGATGAGGATGAGGATGATGCAAGGCCAGTGAAGCAAGTTGGGTTGTTTAGCTTGTTCAAGTACTCAACAAAATGGGATATGTTTCTTGTGTTTTTGGGTTGTGTTGGTGCTCTTATCAATGGAGGGTCTCTTCCTTGGTATTCTTATCTCTTTGGAAAGTTTGTGAATAAGATTGCTAAAGAATCAAAGGACGTTGACAAAGGCCCCATGATGAGAGATGTAGAGAAGATATGCCTGCTTATGACTGGTTTAGCTGCAATAGTAGTAGTTGGAGCATATATGGAGATCACTTGTTGGAGAATGGTGGGGGAAAGATCTGCTCAGAGAATAAGAAGAGAATATCTAAAAGCAGTTCTGCGACAAGACATTAGCTTTTTTGATACTGAGATTGCTGCTGGTGATATTATGCATGGAATTTCAAGTGATGTTGCTGCAATCCAAGAAGTCATGGGGGAGAAGATGGCACATTTCATTCACCATATATGTACCTTCATATGTGGATACACAGTTGGGTTTCTAAGGTCATGGAAAGTAACTCTAGTAGTCTTCTCAGTCATACCATTGATGATGTTAACTGGTATCGCTTACAAGGCTATTTATGTTGGCCTAACCACAAAAGAGGAGTTTTCTTACAGGACAGCTGGTACTGTAGTCGAGCAAGCTGTCAGTTCAATCAGAACAGTATTTTCCTTTGTTGCGGAGGATCATTTGGCTGAAAAATATTCTGCATTAATTGCCAACTTGGTGCCTTTTGGAGCAAAGATTGGCTTTGCTAAGGGTGCAGGAATCGGAGTTATCTATCTTGTTAGTTATTCTACATGGGCGTTGGCTTTCTGGTACGGTTCTCTTTTGGTTGCTAAGGGTGAGATCACTGGAGGTGCTGCCATTGCTTGTTTCTTTGGTGTAAATGTCGGGGGAAGGGGCTTGGCATTGTCACTGTCATACTTTGCTCAGTTTGCACAAGGGACCGTAGCAGCAGGCCGAGTATTTGAAATTATAGATAGAGTTCCAGAAATAGATCCCTACAGCCCAGTAGGTAGGAAACTGTCAAATGTTCGAGGAAGGATTGAATTTAAAGGCATTTATTTCGCATACCCGTCTCGTCCTGAAGCTCCAATTTTTCATTCGCTTAATCTGGTCATTCCATCTTCAAAGACGCTAGCACTTGTTGGCTCTAGCGGAGGTGGAAAGTCTACGATCTTTGCTCTTATAGAGAGATTCTATGACCCTACCAAAGGTACAATAACATTGGATGGTCATGATTTAAGGTCACTTCAGGTGAAGTGGCTAAGGGGTCAGATGGGCATGGTTGGTCAGGAGCCAGTGCTCTTTGCCACCAGCATTTTAGAAAACGTGTTGATGGGGAAAGAGAATGCCACCAAGAAAGAAGCAATTACTGCCTGCATTGCTGCCAATGCTCACAACTTCATTTCTGGACTTCCGCATGGCTATGACACTCAGGTTGGAGACAGAGGAGCACTACTCTCAGGTGGTCAGAAACAAAGAATTGCATTGGCTCGAGCGATGATCAAGGACCCTAGAATCCTTCTCTTGGATGAACCTACCAGTGCCTTAGACCCTGAATCCGAGACTGTAGTCCAACAGGCTATTGACAAAATTTCCTCAGGACGAACAACTATTGTAATTGCTCACAGGCTATCAACTGTAAGAAACTCTCATGCCATTGTTGTTCTTGACAGTGGCTCTGTCATTGAGATTGGTAACCACCGCCAGCTCATGGAAAAAGCCGGGGCTTATTATAGCCTCGTTGAGCTTGCTTCTGATGGAGTGACAAAACCTTTTTCGGAACAATATGATACTGGAATTAGCCCTCAGTTTTCCAGGTTTGACAAATCAGCTTATGATGTATCAAGATCAACATTCATGCAAGATCGAAACCAAGTAGAAGAGAAAGAGGTCCAAAAACCAATTCCAAGAAAGGTTCAGCTTTCAGATATATGGTTGTTACAGAGGCCTGAGCTCCCAATGCTATTAGTTGGATTTCTTTTGGGCATGCATGCAGGTGCGATTCTATCTATCTTCCCTTATATTCTAGGCCAAGCACTTGTAGTTTATTTTGATAAAGAGCCATCAAAGATCAAAACAGGAATCGCGCCCCTTTGCTTAGTACTTGTTGCACTTGGCTTTGGGAATATACTCTTCATGACAGGACAACAAGGCTTGTGTGGCTGGGCTGGAACAAAGCTCACAATGAGAGTGAGAAACCTTCTTTTCCGTTCCATACTTAAACAAGAACCTGGTTGGTTTGATTTTGAAGAAAACTCAAAAGCGGTACTTGTCTCTAGGATTTCAATGGATTCTGTGGCTTTTCGTGCCGTCCATGTTGATCGATTATCAGTCTTGTTTATGGGGTTGAGTTCAGCTATGGTTGGATTGGGCATATCCTTTTGGCTTGAATGGAGGCTAGCTCTTTTGGCTGCTGCTCTCACTCCTTTCACTCTTGGTGCAAGTTACTTGAGCTTGATCATAAATATAGGGCCCAAACTTGACAATGATGCTTATGCCAAGGCTAGCAATATTGCTTCTGGTGCAGTTTCAAACATAAGGACGGTTGCAACATTTTCTGCTCAAGACCAGTTGATCAAGTCCTTTGATAGTGCTTTATCGGAGCCAAAGACAAAATCAATGAGAAGAACGCAAATAATGGGCCTAGCACTCGGGTTCTCTCAAGGTGTTATGTACGGAGCTTACACCTTAACTCTCTTGTTTGGTGCTTACCTTATGAAAGAAAACAAAACTAATTTCGGTGATGTTTACAAAATCTTTCTCATTCTTGTTCTGAGCTCATTTTCTGTGGGACAATTAGCCGGTCTTGCACCAGACACATCTATGGCTGAAACAGCAATTCCAGCAGTTTTTGATATCATGACTCGTAAGCCTTTAATCAGCAGTGGGAGAGGAAAGGATAAGAAGATTGAAAGGTCGAAACGTTTAGATATTCACTTCAAAATGGTGACGTTTGCATATCCATCAAGGCCTGAGGTAACTGTGTTGAGTGAATTTTCCCTCAAGGTCAAAGGTGGTAGCACAGTGGCATTGGTGGGTGGAAGTGGATCGGGAAAATCAACTGTCATATGGTTAATACAAAGGTTTTATGATCCAACTCAAGGGAAGGTGATGATGGGGGGAGTGGATTTGAGGGAGATTGATGTGAAGTGGTTAAGAAGGCAAACAGCTTTAGTTGGTCAAGAGCCTACATTGTTTGCTGGCACTATAAGAGAGAACATTGCTTTTGGTAACCCGGATGCTTCATGGACTGAAATTGAAGATGCTGCAAGAGAAGCTTACATCCACAGCTTCATTAGTGGCCTCCCTCAAGGCTATGAAACTCAGGTTGGTGAGAGTGGGGTTCAGCTATCTGGGGGGCAGAAGCAAAGGATTGCCATAGCAAGAGCTATACTGAAGAAATCAAAGGTACTACTTCTAGATGAAGCAAGTAGTGCACTAGATTTGGAATCTGAGAGACATATACAAGACGCACTTAGGAAAGTCAGCAAAAGGGCCACGACAATCATAGTCGCTCACCGGCTTTCTACTATCAGGGAAGCTGATATGATAGCAGTTATGAAAGATGGTGGCATCACTGAATATGGGAGCCATGATACGCTCATGAAATCTCATCTTAATGGCGTGTATGCCAGCTTGGTGCGTGCAGAAACCGAAGCCAATGCATTTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009966 GO:0009987 GO:0010033 GO:0010218 GO:0010315 GO:0010329 GO:0010540 GO:0010541 GO:0010646 GO:0010817 GO:0010928 GO:0015562 GO:0016020 GO:0016043 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0032501 GO:0032502 GO:0032535 GO:0042221 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044464 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048527 GO:0048528 GO:0048583 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0048859 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080161 GO:0090066 GO:0090567 GO:0090691 GO:0090696 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1513

Amino Acids

165.88

Weight (kDa)

7.89

Isoelectric Point (pI)

35.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	310 - 583	4.4e-56	ABC transporter transmembrane region
ABC_tran	PF00005	655 - 803	1.1e-31	ABC transporter
SMC_N	PF02463	911 - 1476	7.4e-07	RecF/RecN/SMC N terminal domain
ABC_membrane	PF00664	944 - 1215	1.7e-43	ABC transporter transmembrane region
ABC_tran	PF00005	1286 - 1433	2.2e-30	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017342)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35260
malus_domestica	MD09G1177100.v1.1 MD17G1149200.v1.1
prunus_persica	Prupe.3G036400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0146621
rosa_laevigata	RLG00000020211
rosa_multiflora	Rmu_sc0001986.1_g000032
rosa_roxburghii	Rroxscaffold_2G00100000
rosa_rugosa	Rorug02G0395800 Rorug02G0395800
rosa_samantha	Rh2BG464800 Rh2DG473800
rosa_wichuraiana	Rw2G036930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2852
AasI	GACNNNNNNGTC	1 cut(s)	678
Acc36I	ACCTGC	1 cut(s)	2852
AccB1I	GGYRCC	1 cut(s)	1585
AccB7I	CCANNNNNTGG	1 cut(s)	2578
AccBSI	CCGCTC	3 cut(s)	99, 122, 321
AccI	GTMKAC	1 cut(s)	2033
AccII	CGCG	1 cut(s)	2954
AclI	AACGTT	1 cut(s)	3790
AclWI	GGATC	4 cut(s)	1551, 1853, 3922, 3953
AcoI	YGGCCR	2 cut(s)	43, 773
AcsI	RAATTY	5 cut(s)	568, 1254, 1910, 2457, 3860
AcuI	CTGAAG	3 cut(s)	1968, 2099, 4283
AcvI	CACGTG	1 cut(s)	771
AdeI	CACNNNGTG	1 cut(s)	195
AfiI	CCNNNNNNNGG	6 cut(s)	74, 357, 2075, 2392, 2578, 3968
AflII	CTTAAG	1 cut(s)	434
AflIII	ACRYGT	3 cut(s)	189, 768, 2193
AgeI	ACCGGT	1 cut(s)	109
AhlI	ACTAGT	1 cut(s)	788
AjiI	CACGTC	2 cut(s)	192, 291
AjnI	CCWGG	4 cut(s)	212, 300, 2681, 3097
AjuI	GAANNNNNNNTTGG	6 cut(s)	1948, 1980, 2754, 2786, 4179, 4211
AleI	CACNNNNGTG	1 cut(s)	68
Alw21I	GWGCWC	7 cut(s)	290, 952, 2169, 2320, 2823, 3647, 4306
Alw26I	GTCTC	8 cut(s)	150, 418, 972, 1944, 2301, 2423, 3145, 4318
Alw44I	GTGCAC	1 cut(s)	4302
AlwI	GGATC	4 cut(s)	1551, 1853, 3922, 3953
Ama87I	CYCGRG	3 cut(s)	127, 2358, 3518
ApaI	GGGCCC	1 cut(s)	3342
ApaLI	GTGCAC	1 cut(s)	4302
ApoI	RAATTY	5 cut(s)	568, 1254, 1910, 2457, 3860
ArsI	GACNNNNNNTTYG	1 cut(s)	34
AseI	ATTAAT	1 cut(s)	1571
AsiGI	ACCGGT	1 cut(s)	109
AspLEI	GCGC	1 cut(s)	2956
AsuC2I	CCSGG	2 cut(s)	2590, 4103
AsuNHI	GCTAGC	3 cut(s)	1999, 3265, 3371
AvaI	CYCGRG	3 cut(s)	127, 2358, 3518
AvaII	GGWCC	3 cut(s)	1806, 2374, 2758
AxyI	CCTNAGG	2 cut(s)	2464, 3840
BaeGI	GKGCMC	3 cut(s)	316, 3342, 4306
BaeI	ACNNNNGTAYC	2 cut(s)	1476, 1509
BanI	GGYRCC	1 cut(s)	1585
BanII	GRGCYC	5 cut(s)	260, 272, 2823, 3342, 3647
BbrPI	CACGTG	1 cut(s)	771
BbsI	GAAGAC	1 cut(s)	1378
Bbv12I	GWGCWC	7 cut(s)	290, 952, 2169, 2320, 2823, 3647, 4306
BceAI	ACGGC	1 cut(s)	3161
BcgI	CGANNNNNNTGC	5 cut(s)	63, 97, 1909, 1943, 4511
BciT130I	CCWGG	4 cut(s)	214, 302, 2683, 3099
BciVI	GTATCC	1 cut(s)	1334
BclI	TGATCA	6 cut(s)	697, 730, 814, 2367, 3324, 3441
BcnI	CCSGG	2 cut(s)	2590, 4103
BcoDI	GTCTC	8 cut(s)	150, 418, 972, 1944, 2301, 2423, 3145, 4318
BcuI	ACTAGT	1 cut(s)	788
BfmI	CTRYAG	3 cut(s)	1488, 1864, 2433
BfrI	CTTAAG	1 cut(s)	434
BfuAI	ACCTGC	1 cut(s)	2852
BfuI	GTATCC	1 cut(s)	1334
BglII	AGATCT	2 cut(s)	207, 1154
BlpI	GCTNAGC	2 cut(s)	259, 322
BmcAI	AGTACT	3 cut(s)	395, 902, 2970
Bme1390I	CCNGG	6 cut(s)	214, 302, 2590, 2683, 3099, 4103
Bme18I	GGWCC	3 cut(s)	1806, 2374, 2758
BmeT110I	CYCGRG	3 cut(s)	127, 2358, 3518
BmgBI	CACGTC	2 cut(s)	192, 291
BmiI	GGNNCC	8 cut(s)	1045, 1587, 1807, 2160, 2376, 3340, 3471, 4457
BmrFI	CCNGG	6 cut(s)	214, 302, 2590, 2683, 3099, 4103
BmrI	ACTGGG	1 cut(s)	1865
BmsI	GCATC	5 cut(s)	847, 3346, 4096, 4120, 4449
BmtI	GCTAGC	3 cut(s)	2003, 3269, 3375
BmuI	ACTGGG	1 cut(s)	1865
BoxI	GACNNNNGTC	1 cut(s)	2435
BpiI	GAAGAC	1 cut(s)	1378
BplI	GAGNNNNNCTC	4 cut(s)	2307, 2339, 3253, 3285
BpmI	CTGGAG	2 cut(s)	382, 1734
Bpu10I	CCTNAGC	4 cut(s)	1614, 1698, 2129, 2817
Bpu1102I	GCTNAGC	2 cut(s)	259, 322
BpuEI	CTTGAG	6 cut(s)	3337, 3414, 3513, 3854, 3951, 4161
BpuMI	CCSGG	2 cut(s)	2590, 4103
Bsa29I	ATCGAT	2 cut(s)	618, 3190
BsaAI	YACGTR	1 cut(s)	771
BsaI	GGTCTC	2 cut(s)	150, 972
BsaJI	CCNNGG	8 cut(s)	177, 272, 530, 676, 747, 974, 2589, 3366
BsaWI	WCCGGW	1 cut(s)	109
BsaXI	ACNNNNNCTCC	6 cut(s)	326, 356, 380, 410, 469, 499
Bsc4I	CCNNNNNNNGG	6 cut(s)	74, 357, 2075, 2392, 2578, 3968
Bse118I	RCCGGY	3 cut(s)	109, 3668, 4386
Bse21I	CCTNAGG	2 cut(s)	2464, 3840
Bse3DI	GCAATG	3 cut(s)	1725, 2244, 4086
BseBI	CCWGG	4 cut(s)	214, 302, 2683, 3099
BseCI	ATCGAT	2 cut(s)	618, 3190
BseDI	CCNNGG	8 cut(s)	177, 272, 530, 676, 747, 974, 2589, 3366
BseGI	GGATG	9 cut(s)	10, 841, 847, 853, 859, 2098, 2401, 4111, 4149
BseLI	CCNNNNNNNGG	6 cut(s)	74, 357, 2075, 2392, 2578, 3968
BseMI	GCAATG	3 cut(s)	1725, 2244, 4086
BseRI	GAGGAG	3 cut(s)	4, 1475, 2328
BseSI	GKGCMC	3 cut(s)	316, 3342, 4306
BseYI	CCCAGC	3 cut(s)	306, 557, 3030
BsgI	GTGCAG	3 cut(s)	1641, 3411, 4533
Bsh1236I	CGCG	1 cut(s)	2954
BshNI	GGYRCC	1 cut(s)	1585
BshTI	ACCGGT	1 cut(s)	109
BshVI	ATCGAT	2 cut(s)	618, 3190
BsiHKAI	GWGCWC	7 cut(s)	290, 952, 2169, 2320, 2823, 3647, 4306
BsiHKCI	CYCGRG	3 cut(s)	127, 2358, 3518
BsiSI	CCGG	6 cut(s)	42, 110, 2589, 3669, 4103, 4387
BslFI	GGGAC	3 cut(s)	467, 1819, 3672
BslI	CCNNNNNNNGG	6 cut(s)	74, 357, 2075, 2392, 2578, 3968
BsmAI	GTCTC	8 cut(s)	150, 418, 972, 1944, 2301, 2423, 3145, 4318
BsmBI	CGTCTC	1 cut(s)	1944
BsmFI	GGGAC	3 cut(s)	467, 1819, 3672
BsmI	GAATGC	2 cut(s)	778, 2218
Bso31I	GGTCTC	2 cut(s)	150, 972
BsoBI	CYCGRG	3 cut(s)	127, 2358, 3518
Bsp120I	GGGCCC	1 cut(s)	3338
Bsp1720I	GCTNAGC	2 cut(s)	259, 322
Bsp19I	CCATGG	3 cut(s)	272, 676, 747
BspDI	ATCGAT	2 cut(s)	618, 3190
BspFNI	CGCG	1 cut(s)	2954
BspHI	TCATGA	5 cut(s)	763, 2098, 3003, 3726, 4470
BspLI	GGNNCC	8 cut(s)	1045, 1587, 1807, 2160, 2376, 3340, 3471, 4457
BspMI	ACCTGC	1 cut(s)	2852
BspOI	GCTAGC	3 cut(s)	2003, 3269, 3375
BspPI	GGATC	4 cut(s)	1551, 1853, 3922, 3953
BspT107I	GGYRCC	1 cut(s)	1585
BspTI	CTTAAG	1 cut(s)	434
BspTNI	GGTCTC	2 cut(s)	150, 972
BsrBI	CCGCTC	3 cut(s)	99, 122, 321
BsrDI	GCAATG	3 cut(s)	1725, 2244, 4086
BsrFI	RCCGGY	3 cut(s)	109, 3668, 4386
BssAI	RCCGGY	3 cut(s)	109, 3668, 4386
BssECI	CCNNGG	8 cut(s)	177, 272, 530, 676, 747, 974, 2589, 3366
BssT1I	CCWWGG	6 cut(s)	272, 530, 676, 747, 974, 3366
Bst2UI	CCWGG	4 cut(s)	214, 302, 2683, 3099
Bst6I	CTCTTC	4 cut(s)	975, 1168, 2742, 3005
BstAFI	CTTAAG	1 cut(s)	434
BstAPI	GCANNNNNTGC	3 cut(s)	1574, 2237, 4301
BstBAI	YACGTR	1 cut(s)	771
BstDSI	CCRYGG	3 cut(s)	272, 676, 747
BstEII	GGTNACC	2 cut(s)	2561, 4097
BstENI	CCTNNNNNAGG	2 cut(s)	72, 2073
BstF5I	GGATG	9 cut(s)	10, 841, 847, 853, 859, 2098, 2401, 4111, 4149
BstFNI	CGCG	1 cut(s)	2954
BstHHI	GCGC	1 cut(s)	2956
BstMAI	GTCTC	8 cut(s)	150, 418, 972, 1944, 2301, 2423, 3145, 4318
BstNI	CCWGG	4 cut(s)	214, 302, 2683, 3099
BstNSI	RCATGY	2 cut(s)	2857, 2861
BstPAI	GACNNNNGTC	1 cut(s)	2435
BstPI	GGTNACC	2 cut(s)	2561, 4097
BstSCI	CCNGG	6 cut(s)	212, 300, 2588, 2681, 3097, 4101
BstSFI	CTRYAG	3 cut(s)	1488, 1864, 2433
BstSLI	GKGCMC	3 cut(s)	316, 3342, 4306
BstUI	CGCG	1 cut(s)	2954
BstV2I	GAAGAC	1 cut(s)	1378
BstX2I	RGATCY	3 cut(s)	207, 1154, 1858
BstXI	CCANNNNNNTGG	2 cut(s)	70, 683
BstYI	RGATCY	3 cut(s)	207, 1154, 1858
Bsu15I	ATCGAT	2 cut(s)	618, 3190
Bsu36I	CCTNAGG	2 cut(s)	2464, 3840
BsuI	GTATCC	1 cut(s)	1334
BsuTUI	ATCGAT	2 cut(s)	618, 3190
BtgI	CCRYGG	3 cut(s)	272, 676, 747
BtgZI	GCGATG	1 cut(s)	2378
BtrI	CACGTC	2 cut(s)	192, 291
BtsCI	GGATG	9 cut(s)	10, 841, 847, 853, 859, 2098, 2401, 4111, 4149
BtsI	GCAGTG	2 cut(s)	586, 3757
BveI	ACCTGC	1 cut(s)	2852
CciI	TCATGA	5 cut(s)	763, 2098, 3003, 3726, 4470
CfoI	GCGC	1 cut(s)	2956
Cfr10I	RCCGGY	3 cut(s)	109, 3668, 4386
ClaI	ATCGAT	2 cut(s)	618, 3190
CseI	GACGC	2 cut(s)	2005, 4346
CsiI	ACCWGGT	1 cut(s)	3097
CspAI	ACCGGT	1 cut(s)	109
DraI	TTTAAA	1 cut(s)	1915
DraIII	CACNNNGTG	1 cut(s)	195
DrdI	GACNNNNNNGTC	1 cut(s)	678
DseDI	GACNNNNNNGTC	1 cut(s)	678
EaeI	YGGCCR	2 cut(s)	43, 773
Eam1104I	CTCTTC	4 cut(s)	975, 1168, 2742, 3005
EarI	CTCTTC	4 cut(s)	975, 1168, 2742, 3005
Ecl136II	GAGCTC	2 cut(s)	2821, 3645
Eco130I	CCWWGG	6 cut(s)	272, 530, 676, 747, 974, 3366
Eco147I	AGGCCT	2 cut(s)	2815, 3838
Eco24I	GRGCYC	5 cut(s)	260, 272, 2823, 3342, 3647
Eco31I	GGTCTC	2 cut(s)	150, 972
Eco32I	GATATC	1 cut(s)	3724
Eco47I	GGWCC	3 cut(s)	1806, 2374, 2758
Eco53kI	GAGCTC	2 cut(s)	2821, 3645
Eco57I	CTGAAG	3 cut(s)	1968, 2099, 4283
Eco72I	CACGTG	1 cut(s)	771
Eco81I	CCTNAGG	2 cut(s)	2464, 3840
Eco88I	CYCGRG	3 cut(s)	127, 2358, 3518
Eco91I	GGTNACC	2 cut(s)	2561, 4097
EcoICRI	GAGCTC	2 cut(s)	2821, 3645
EcoNI	CCTNNNNNAGG	2 cut(s)	72, 2073
EcoO109I	RGGNCCY	3 cut(s)	1043, 2374, 3338
EcoO65I	GGTNACC	2 cut(s)	2561, 4097
EcoRII	CCWGG	4 cut(s)	212, 300, 2681, 3097
EcoRV	GATATC	1 cut(s)	3724
EcoT14I	CCWWGG	6 cut(s)	272, 530, 676, 747, 974, 3366
EcoT22I	ATGCAT	3 cut(s)	1251, 2859, 4534
EcoT38I	GRGCYC	5 cut(s)	260, 272, 2823, 3342, 3647
ErhI	CCWWGG	6 cut(s)	272, 530, 676, 747, 974, 3366
Esp3I	CGTCTC	1 cut(s)	1944
FaqI	GGGAC	3 cut(s)	467, 1819, 3672
FauI	CCCGC	3 cut(s)	104, 350, 353
FauNDI	CATATG	2 cut(s)	1334, 3935
FbaI	TGATCA	6 cut(s)	697, 730, 814, 2367, 3324, 3441
FblI	GTMKAC	1 cut(s)	2033
FokI	GGATG	9 cut(s)	17, 848, 854, 860, 866, 2105, 2408, 4118, 4136
FriOI	GRGCYC	5 cut(s)	260, 272, 2823, 3342, 3647
GlaI	GCGC	1 cut(s)	2955
GsaI	CCCAGC	3 cut(s)	310, 561, 3034
GsuI	CTGGAG	2 cut(s)	382, 1734
HapII	CCGG	6 cut(s)	42, 110, 2589, 3669, 4103, 4387
HgaI	GACGC	2 cut(s)	2005, 4346
HhaI	GCGC	1 cut(s)	2956
Hin6I	GCGC	1 cut(s)	2954
HinP1I	GCGC	1 cut(s)	2954
HincII	GTYRAC	2 cut(s)	1036, 1413
HindII	GTYRAC	2 cut(s)	1036, 1413
HindIII	AAGCTT	1 cut(s)	4143
HpaI	GTTAAC	1 cut(s)	1413
HpaII	CCGG	6 cut(s)	42, 110, 2589, 3669, 4103, 4387
Hpy166II	GTNNAC	6 cut(s)	671, 1036, 1413, 2034, 3616, 4304
Hpy8I	GTNNAC	6 cut(s)	671, 1036, 1413, 2034, 3616, 4304
Hpy99I	CGWCG	3 cut(s)	85, 196, 232
HpyAV	CCTTC	8 cut(s)	135, 1339, 1752, 1896, 2396, 3077, 3967, 4020
HpyCH4IV	ACGT	9 cut(s)	55, 191, 290, 444, 770, 1032, 2193, 3790, 3818
HpySE526I	ACGT	9 cut(s)	55, 191, 290, 444, 770, 1032, 2193, 3790, 3818
HspAI	GCGC	1 cut(s)	2954
Ksp22I	TGATCA	6 cut(s)	697, 730, 814, 2367, 3324, 3441
KspAI	GTTAAC	1 cut(s)	1413
LweI	GCATC	5 cut(s)	847, 3346, 4096, 4120, 4449
MabI	ACCWGGT	1 cut(s)	3097
MaeII	ACGT	9 cut(s)	55, 191, 290, 444, 770, 1032, 2193, 3790, 3818
MbiI	CCGCTC	3 cut(s)	99, 122, 321
MflI	RGATCY	3 cut(s)	207, 1154, 1858
MlyI	GAGTC	3 cut(s)	5, 86, 3724
MmeI	TCCRAC	9 cut(s)	185, 516, 1090, 1115, 2284, 2464, 2818, 3211, 3986
Mph1103I	ATGCAT	3 cut(s)	1251, 2859, 4534
MslI	CAYNNNNRTG	8 cut(s)	68, 681, 732, 762, 2289, 2862, 3689, 4485
MspA1I	CMGCKG	3 cut(s)	557, 576, 1481
MspCI	CTTAAG	1 cut(s)	434
MspI	CCGG	6 cut(s)	42, 110, 2589, 3669, 4103, 4387
MspR9I	CCNGG	6 cut(s)	214, 302, 2590, 2683, 3099, 4103
Mva1269I	GAATGC	2 cut(s)	778, 2218
MvaI	CCWGG	4 cut(s)	214, 302, 2683, 3099
MvnI	CGCG	1 cut(s)	2954
NciI	CCSGG	2 cut(s)	2590, 4103
NcoI	CCATGG	3 cut(s)	272, 676, 747
NdeI	CATATG	2 cut(s)	1334, 3935
NheI	GCTAGC	3 cut(s)	1999, 3265, 3371
NlaIV	GGNNCC	8 cut(s)	1045, 1587, 1807, 2160, 2376, 3340, 3471, 4457
NmeAIII	GCCGAG	1 cut(s)	1847
NmuCI	GTSAC	7 cut(s)	412, 741, 791, 1773, 2109, 2629, 3814
NsiI	ATGCAT	3 cut(s)	1251, 2859, 4534
NspI	RCATGY	2 cut(s)	2857, 2861
OliI	CACNNNNGTG	1 cut(s)	68
PaeI	GCATGC	2 cut(s)	2857, 2861
PaeR7I	CTCGAG	2 cut(s)	127, 2358
PagI	TCATGA	5 cut(s)	763, 2098, 3003, 3726, 4470
PaqCI	CACCTGC	1 cut(s)	2852
PceI	AGGCCT	2 cut(s)	2815, 3838
PctI	GAATGC	2 cut(s)	778, 2218
PfeI	GAWTC	9 cut(s)	1022, 1626, 2058, 2382, 2423, 2869, 2949, 3158, 4316
PflMI	CCANNNNNTGG	1 cut(s)	2578
PinAI	ACCGGT	1 cut(s)	109
PleI	GAGTC	3 cut(s)	5, 86, 3724
PmaCI	CACGTG	1 cut(s)	771
PmlI	CACGTG	1 cut(s)	771
PpsI	GAGTC	3 cut(s)	5, 86, 3724
Ppu21I	YACGTR	1 cut(s)	771
PpuMI	RGGWCCY	1 cut(s)	2374
PshAI	GACNNNNGTC	1 cut(s)	2435
PshBI	ATTAAT	1 cut(s)	1571
Psp124BI	GAGCTC	2 cut(s)	2823, 3647
Psp1406I	AACGTT	1 cut(s)	3790
Psp5II	RGGWCCY	1 cut(s)	2374
Psp6I	CCWGG	4 cut(s)	212, 300, 2681, 3097
PspCI	CACGTG	1 cut(s)	771
PspEI	GGTNACC	2 cut(s)	2561, 4097
PspFI	CCCAGC	3 cut(s)	306, 557, 3030
PspGI	CCWGG	4 cut(s)	212, 300, 2681, 3097
PspN4I	GGNNCC	8 cut(s)	1045, 1587, 1807, 2160, 2376, 3340, 3471, 4457
PspOMI	GGGCCC	1 cut(s)	3338
PspPPI	RGGWCCY	1 cut(s)	2374
PspXI	VCTCGAGB	1 cut(s)	2358
PsuI	RGATCY	3 cut(s)	207, 1154, 1858
PvuII	CAGCTG	2 cut(s)	557, 1481
RseI	CAYNNNNRTG	8 cut(s)	68, 681, 732, 762, 2289, 2862, 3689, 4485
SacI	GAGCTC	2 cut(s)	2823, 3647
ScaI	AGTACT	3 cut(s)	395, 902, 2970
SchI	GAGTC	3 cut(s)	5, 86, 3724
ScrFI	CCNGG	6 cut(s)	214, 302, 2590, 2683, 3099, 4103
SexAI	ACCWGGT	1 cut(s)	3097
SfaNI	GCATC	5 cut(s)	847, 3346, 4096, 4120, 4449
SfcI	CTRYAG	3 cut(s)	1488, 1864, 2433
Sfr274I	CTCGAG	2 cut(s)	127, 2358
SinI	GGWCC	3 cut(s)	1806, 2374, 2758
SlaI	CTCGAG	2 cut(s)	127, 2358
SmiMI	CAYNNNNRTG	8 cut(s)	68, 681, 732, 762, 2289, 2862, 3689, 4485
SmlI	CTYRAG	9 cut(s)	127, 434, 2358, 3316, 3429, 3528, 3869, 3966, 4176
SmoI	CTYRAG	9 cut(s)	127, 434, 2358, 3316, 3429, 3528, 3869, 3966, 4176
SpeI	ACTAGT	1 cut(s)	788
SphI	GCATGC	2 cut(s)	2857, 2861
SseBI	AGGCCT	2 cut(s)	2815, 3838
SspI	AATATT	3 cut(s)	383, 1562, 3379
SstI	GAGCTC	2 cut(s)	2823, 3647
StuI	AGGCCT	2 cut(s)	2815, 3838
StyD4I	CCNGG	6 cut(s)	212, 300, 2588, 2681, 3097, 4101
StyI	CCWWGG	6 cut(s)	272, 530, 676, 747, 974, 3366
TaiI	ACGT	9 cut(s)	58, 194, 293, 447, 773, 1035, 2196, 3793, 3821
TaqI	TCGA	9 cut(s)	21, 128, 618, 1494, 1897, 2359, 2734, 3190, 3785
TatI	WGTACW	3 cut(s)	393, 900, 2968
TauI	GCSGC	2 cut(s)	579, 778
TfiI	GAWTC	9 cut(s)	1022, 1626, 2058, 2382, 2423, 2869, 2949, 3158, 4316
TseFI	GTSAC	7 cut(s)	412, 741, 791, 1773, 2109, 2629, 3814
Tsp45I	GTSAC	7 cut(s)	412, 741, 791, 1773, 2109, 2629, 3814
TspGWI	ACGGA	2 cut(s)	3065, 3558
Van91I	CCANNNNNTGG	1 cut(s)	2578
Vha464I	CTTAAG	1 cut(s)	434
VneI	GTGCAC	1 cut(s)	4302
VpaK11BI	GGWCC	3 cut(s)	1806, 2374, 2758
VspI	ATTAAT	1 cut(s)	1571
XagI	CCTNNNNNAGG	2 cut(s)	72, 2073
XapI	RAATTY	5 cut(s)	568, 1254, 1910, 2457, 3860
XbaI	TCTAGA	1 cut(s)	4285
XceI	RCATGY	2 cut(s)	2857, 2861
XhoI	CTCGAG	2 cut(s)	127, 2358
XmiI	GTMKAC	1 cut(s)	2033
ZrmI	AGTACT	3 cut(s)	395, 902, 2970
Zsp2I	ATGCAT	3 cut(s)	1251, 2859, 4534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.