Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0017342

Physical Location & Seq

Genomic Coordinates Reverse (-)

68569644 .. 68575782

Gene Structure 6139 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG473800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4545 bp

ATGGATGCAGACTCCTCATTCGAGCTGGAAAACTCTATTTCCGGCCACCTCCCACGTTACACCACCCCTGTGGCAGGCTCGTCGCATTATGACTCCCGCTCATTCACACCGGTTAGCTCCGCTCACTTGAGAACTCCGAGAACTCCCAGAAGGTCTCGCCACCCCACTCCAACTACGCCATTCGCCTCGGACAATGACATGTCGTGGCAAGGCGAGATCTCCTGGCAGTTTGAGCCGACGGGGTGGAACGAGACTCGCAACTTGGGCTCAGCTATGAGCCCATGGGCCACCGTGAGCACGTCCTCTCGCCAGGCCCAGCGTGCCCACCGCTCAGCAAATTACTACTACCTCTCCCGTACCACTGGCGGGTTTCGTACCTCCCCAAATCAATATTACAACGAGTACTCCAGCTATGGTGCTGTGACCGATGGGAGACTTGAGCTTAAGAGCTACGTTGCTAGGGACAATGAAAGCTCAACTTTTGGGAGGAGTAGTTACAATTATGGGGAGCAGAAGAAACCCTTTAGGGCAGTTACTCCAAGGTTGGAGGGTATTAAAGAAGCAGCTGGGAGTGGAAATTTCAGTGGCAGTGGCCCTTTGGCTAAGAAAGATTTGCTTGGTTTGATCGATTATCAAACAGCAGAAGATGATGATGAAGATGAGAGTTTGGGTCATAGTGGACACCATGGTCATGGCCTATATCTTGATCATGGCCATAGGCCATCACACAAAGGACATGATCATGGGTTGTCACCCATGGGTCATCATGGTCATGACACGTGGCCGCATTCAATTACTAGTCACGATATTGATGATGAGAGTGATCAGGGTTCTGGGTATGAGGATGAGGATGAGGATGATGCAAGGCCAGTGAAGCAAGTTGGGTTGTTTAGCTTGTTCAAGTACTCAACAAAATGGGATATGTTTCTTGTGTTTTTGGGTTGTGTTGGTGCTCTTATCAATGGAGGGTCTCTTCCTTGGTATTCTTATCTCTTTGGAAAGTTTGTGAATAAGATTGCTAAAGAATCAAAGGACGTTGACAAAGGCCCCATGATGAGAGATGTAGAGAAGATATGCCTGCTTATGACTGGTTTAGCTGCAATAGTAGTAGTTGGAGCATATATGGAGATCACTTGTTGGAGAATGGTGGGGGAGAGATCTGCTCAGAGAATAAGAAGAGAATATCTAAAAGCAGTTCTGCGACAAGACATTAGCTTTTTTGATACTGAGATTGCTGCTGGTGATATTATGCATGGAATTTCAAGTGATGTTGCTGCAATCCAAGAAGTCATGGGGGAGAAGATGGCACATTTCATTCACCATATATGTACCTTCATATGTGGATACACAGTGGGGTTTCTAAGGTCATGGAAAGTAACTCTAGTAGTCTTCTCAGTCATACCATTGATGATGTTAACTGGTATCGCTTACAAGGCTATTTATGTTGGCCTAACCACAAAAGAGGAGTTTTCTTACAGGACAGCTGGTACTGTAGTCGAGCAAGCTGTCAGTTCAATCAGAACAGTATTTTCCTTTGTTGCGGAGGATCATTTGGCTGAAAAATATTCTGCATTAATTGCCAACTTGGTGCCTTTTGGAGCAAAGATTGGCTTTGCTAAGGGTGCAGGAATCGGAGTTATCTATCTTGTTAGTTATTCGACATGGGCGTTGGCTTTCTGGTACGGTTCTCTTTTGGTTGCTAAGGGTGAGATCACTGGAGGTGCTGCCATTGCTTGTTTCTTTGGTGTAAATGTCGGGGGAAGGGGCTTGGCATTGTCACTGTCATACTTTGCTCAGTTTGCACAAGGGACCGTAGCAGCAGGCCGAGTATTTGAAATTATAGATAGAGTTCCAGAAATAGATCCCTACAGCCCAGTAGGTAGGAAACTGTCAAATGTTCGAGGAAGGATTGAATTTAAAGGCATTTATTTCGCATACCCGTCTCGTCCTGAAGCTCCAATTTTTCATTCGCTTAATCTGGTCATTCCATCTTCAAAGACGCTAGCACTTGTTGGCTCTAGCGGAGGTGGAAAGTCTACGATCTTTGCTCTTATAGAGAGATTCTATGACCCTACCAAAGGTACAATAACGTTGGATGGTCATGATTTAAGGTCACTTCAGGTGAAGTGGCTAAGGGGTCAGATGGGCATGGTTGGTCAGGAGCCAGTGCTCTTTGCCACCAGCATTTTAGAAAACGTGTTGATGGGGAAAGAGAATGCCACCAAGAAAGAAGCAATTACTGCCTGCATTGCTGCCAATGCTCACAGCTTCATTTCTGGACTTCCGCATGGCTATGACACTCAGGTTGGAGACAGAGGAACACTACTCTCAGGTGGTCAGAAACAAAGAATTGCATTGGCTCGAGCGATGATCAAGGACCCTAGAATCCTTCTCTTGGATGAACCTACCAGTGCCTTAGACCCTGAATCCGAGACTGTAGTCCAACAGGCTATTGACAAAATTTCCTCAGGACGAACAACTATTGTAATTGCTCACAGGCTATCAACTGTAAGAAACTCTCATGCCATTGTTGTTCTTGACAGTGGCTCTGTCATTGAGATTGGTAACCACCGCCAGCTCATGGAAAAAGCCGGGGCGTATTATAGCCTCGTTGAGCTTGCTTCTGATGGAGTGACAAAACCTTTTTCGGAACAATATGATACTGGAATTAGCCCTCAGTTTTCCAGGTTTGACAAATCAGCTTATGATGTATCAAGATCAACATTCATGCAAGATCGAAACCAAGTAGAAGAGAAAGAGGTCCAAAAACCAATTCCAAGAAAGGTTCAGCTTTCAGATATATGGTTGTTACAGAGGCCTGAGCTCCCAATGCTATTAGTTGGATTTCTTTTGGGCATGCATGCAGGTGCGATTCTATCTATCTTCCCTTATATTCTAGGCCAAGCACTTGTAGTTTATTTTGATAAAGAGCCATCAAAGATCAAAACAGGAATCGCACCCCTTTGCTTAGTACTTGTTGCACTTGGCTTTGGGAATATACTCTTCATGACAGGACAACAAGGCTTGTGTGGCTGGGCTGGAACAAAGCTCACAATGAGAGTGAGAAACCTTCTTTTCCGTTCCATACTTAAACAAGAACCTGGTTGGTTTGATTTTGAAGAAAACTCAAAAGCGGTACTTGTCTCTAGGATTTCAATGGATTCTGTGGCTTTTCGTGCCGTCCATGTTGATCGATTATCAGTCTTGTTTATGGGGTTGAGTTCAGCTATGGTTGGATTGGGCATATCCTTTTGGCTTGAATGGAGGCTAGCTCTTTTGGCTGCTGCTCTCACTCCTTTCACTCTTGGTGCAAGTTACTTGAGCTTGATCATAAATATAGGGCCCAAACTTGACAATGATGCTTATGCCAAGGCTAGCAATATTGCTTCTGGTGCAGTTTCAAACATAAGGACGGTTGCAACATTTTCTGCTCAAGACCAGTTGATCAAGTCCTTTGATAGTGCTTTATCGGAGCCAAAGACAAAATCAATGAGAAGAACGCAAATAATGGGCCTAGCACTCGGGTTCTCTCAAGGTGTTATGTACGGAGCTTACACCTTAACTCTCTTGTTTGGTGCTTACCTTATGAAAGAAAACAAAACTAATTTCGGTGATGTTTACAAAATCTTTCTCATTCTTGTTCTGAGCTCATTTTCTGTGGGACAATTAGCCGGTCTTGCACCAGACACATCTATGGCTGAAACAGCAATTCCAGCAGTTTTTGATATCATGACTCGTAAGCCTTTAATCAGCAGTGGGAGAGGAAAGGATAAGAAGATTGAAAGGTTGAAACGTTTAGATATTCACTTCAAAATGGTGACGTTTGCATATCCATCAAGGCCTGAGGTAACTGTGTTGAGTGAATTTTCCCTCAAGGTCAAAGGTGGTAGCACAGTGGCATTGGTGGGTGGAAGTGGATCGGGAAAATCAACTGTCATATGGTTAATACAAAGGTTTTATGATCCAACTCAAGGGAAGGTGATGATGGGGGGAGTGGATTTGAGGGAGATTGATGTGAAGTGGTTAAGAAGGCAAACAGCTTTAGTTGGTCAAGAGCCTACATTGTTTGCTGGCACTATAAGAGGGAACATTGCTTTTGGTAACCCGGATGCTTCATGGACTGAAATTGAAGATGCTGCAAGAGAAGCTTACATCCACAGCTTCATTAGTGGCCTCCCTCAAGGCTATGAAACTCAGGTTGGTGAGAGTGGGGTTCAGCTATCTGGGGGGCAGAAGCAAAGGATTGCCATAGCAAGAGCTATACTGAAGAAATCAAAGGTACTACTTCTAGATGAAGCAAGTAGTGCACTAGATTTGGAATCTGAGAGACATATACAAGACGCACTTAGGAAAGTCAGCAAAAGGGCCACGACAATCATAGTCGCTCACCGGCTTTCTACTATCAGGGAAGCTGATATGATAGCAGTTATGAAAGATGGTGGCATCACTGAATATGGGAGCCATGATACGCTCATGAAATCTCATCTTAATGGCGTGTATGCCAGCTTGGTGCGCGCAGAAACCGAAGCCAATGCATTTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009966 GO:0009987 GO:0010033 GO:0010218 GO:0010315 GO:0010329 GO:0010540 GO:0010541 GO:0010646 GO:0010817 GO:0010928 GO:0015562 GO:0016020 GO:0016043 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0032501 GO:0032502 GO:0032535 GO:0042221 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044464 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048527 GO:0048528 GO:0048583 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0048859 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080161 GO:0090066 GO:0090567 GO:0090691 GO:0090696 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1514

Amino Acids

166.06

Weight (kDa)

8.42

Isoelectric Point (pI)

35.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	311 - 584	4.4e-56	ABC transporter transmembrane region
ABC_tran	PF00005	656 - 804	1.3e-31	ABC transporter
SMC_N	PF02463	909 - 1477	7.2e-07	RecF/RecN/SMC N terminal domain
ABC_membrane	PF00664	945 - 1216	1.7e-43	ABC transporter transmembrane region
ABC_tran	PF00005	1287 - 1434	1.6e-29	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017342)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35260
malus_domestica	MD09G1177100.v1.1 MD17G1149200.v1.1
prunus_persica	Prupe.3G036400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0146621
rosa_laevigata	RLG00000020211
rosa_multiflora	Rmu_sc0001986.1_g000032
rosa_roxburghii	Rroxscaffold_2G00100000
rosa_rugosa	Rorug02G0395800 Rorug02G0395800
rosa_samantha	Rh2BG464800 Rh2DG473800
rosa_wichuraiana	Rw2G036930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2855
AasI	GACNNNNNNGTC	1 cut(s)	687
Acc36I	ACCTGC	1 cut(s)	2855
AccB1I	GGYRCC	1 cut(s)	1588
AccB7I	CCANNNNNTGG	1 cut(s)	2581
AccBSI	CCGCTC	3 cut(s)	99, 122, 330
AccI	GTMKAC	1 cut(s)	2036
AccII	CGCG	1 cut(s)	4515
AclI	AACGTT	2 cut(s)	2090, 3793
AclWI	GGATC	4 cut(s)	1554, 1856, 3925, 3956
AcoI	YGGCCR	3 cut(s)	43, 712, 782
AcsI	RAATTY	5 cut(s)	577, 1257, 1913, 2460, 3863
AcuI	CTGAAG	3 cut(s)	1971, 2102, 4286
AcvI	CACGTG	1 cut(s)	780
AfiI	CCNNNNNNNGG	6 cut(s)	74, 366, 2078, 2395, 2581, 3971
AflII	CTTAAG	1 cut(s)	443
AflIII	ACRYGT	3 cut(s)	198, 777, 2196
AgeI	ACCGGT	1 cut(s)	109
AhlI	ACTAGT	1 cut(s)	797
AjiI	CACGTC	1 cut(s)	300
AjnI	CCWGG	4 cut(s)	221, 309, 2684, 3100
AjuI	GAANNNNNNNTTGG	6 cut(s)	1951, 1983, 2757, 2789, 4182, 4214
AleI	CACNNNNGTG	1 cut(s)	68
Alw21I	GWGCWC	6 cut(s)	299, 955, 2172, 2826, 3650, 4309
Alw26I	GTCTC	9 cut(s)	159, 245, 427, 975, 1947, 2304, 2426, 3148, 4321
Alw44I	GTGCAC	1 cut(s)	4305
AlwI	GGATC	4 cut(s)	1554, 1856, 3925, 3956
Ama87I	CYCGRG	2 cut(s)	2361, 3521
ApaI	GGGCCC	1 cut(s)	3345
ApaLI	GTGCAC	1 cut(s)	4305
ApoI	RAATTY	5 cut(s)	577, 1257, 1913, 2460, 3863
ArsI	GACNNNNNNTTYG	1 cut(s)	34
AseI	ATTAAT	1 cut(s)	1574
AsiGI	ACCGGT	1 cut(s)	109
AspLEI	GCGC	2 cut(s)	4515, 4517
AsuC2I	CCSGG	2 cut(s)	2593, 4106
AsuNHI	GCTAGC	3 cut(s)	2002, 3268, 3374
AvaI	CYCGRG	2 cut(s)	2361, 3521
AvaII	GGWCC	3 cut(s)	1809, 2377, 2761
AxyI	CCTNAGG	2 cut(s)	2467, 3843
BaeGI	GKGCMC	3 cut(s)	325, 3345, 4309
BaeI	ACNNNNGTAYC	2 cut(s)	1479, 1512
BalI	TGGCCA	1 cut(s)	714
BanI	GGYRCC	1 cut(s)	1588
BanII	GRGCYC	5 cut(s)	269, 281, 2826, 3345, 3650
BbrPI	CACGTG	1 cut(s)	780
BbsI	GAAGAC	1 cut(s)	1381
Bbv12I	GWGCWC	6 cut(s)	299, 955, 2172, 2826, 3650, 4309
BceAI	ACGGC	1 cut(s)	3164
BcgI	CGANNNNNNTGC	5 cut(s)	63, 97, 1912, 1946, 4514
BciT130I	CCWGG	4 cut(s)	223, 311, 2686, 3102
BciVI	GTATCC	1 cut(s)	1337
BclI	TGATCA	6 cut(s)	706, 739, 823, 2370, 3327, 3444
BcnI	CCSGG	2 cut(s)	2593, 4106
BcoDI	GTCTC	9 cut(s)	159, 245, 427, 975, 1947, 2304, 2426, 3148, 4321
BcuI	ACTAGT	1 cut(s)	797
BfmI	CTRYAG	3 cut(s)	1491, 1867, 2436
BfrI	CTTAAG	1 cut(s)	443
BfuAI	ACCTGC	1 cut(s)	2855
BfuI	GTATCC	1 cut(s)	1337
BglII	AGATCT	2 cut(s)	216, 1157
BlpI	GCTNAGC	2 cut(s)	268, 331
BmcAI	AGTACT	3 cut(s)	404, 905, 2973
Bme1390I	CCNGG	6 cut(s)	223, 311, 2593, 2686, 3102, 4106
Bme18I	GGWCC	3 cut(s)	1809, 2377, 2761
BmeT110I	CYCGRG	2 cut(s)	2361, 3521
BmgBI	CACGTC	1 cut(s)	300
BmiI	GGNNCC	8 cut(s)	1048, 1590, 1810, 2163, 2379, 3343, 3474, 4460
BmrFI	CCNGG	6 cut(s)	223, 311, 2593, 2686, 3102, 4106
BmrI	ACTGGG	1 cut(s)	1868
BmsI	GCATC	5 cut(s)	850, 3349, 4099, 4123, 4452
BmtI	GCTAGC	3 cut(s)	2006, 3272, 3378
BmuI	ACTGGG	1 cut(s)	1868
BoxI	GACNNNNGTC	1 cut(s)	2438
BpiI	GAAGAC	1 cut(s)	1381
BplI	GAGNNNNNCTC	4 cut(s)	1147, 1179, 3256, 3288
BpmI	CTGGAG	2 cut(s)	391, 1737
Bpu10I	CCTNAGC	4 cut(s)	1617, 1701, 2132, 2820
Bpu1102I	GCTNAGC	2 cut(s)	268, 331
BpuEI	CTTGAG	8 cut(s)	148, 458, 3340, 3417, 3516, 3857, 3954, 4164
BpuMI	CCSGG	2 cut(s)	2593, 4106
Bsa29I	ATCGAT	2 cut(s)	627, 3193
BsaAI	YACGTR	1 cut(s)	780
BsaI	GGTCTC	2 cut(s)	159, 975
BsaJI	CCNNGG	8 cut(s)	186, 281, 539, 685, 756, 977, 2592, 3369
BsaWI	WCCGGW	1 cut(s)	109
BsaXI	ACNNNNNCTCC	6 cut(s)	335, 365, 389, 419, 478, 508
Bsc4I	CCNNNNNNNGG	6 cut(s)	74, 366, 2078, 2395, 2581, 3971
Bse118I	RCCGGY	3 cut(s)	109, 3671, 4389
Bse21I	CCTNAGG	2 cut(s)	2467, 3843
Bse3DI	GCAATG	3 cut(s)	1728, 2247, 4089
BseBI	CCWGG	4 cut(s)	223, 311, 2686, 3102
BseCI	ATCGAT	2 cut(s)	627, 3193
BseDI	CCNNGG	8 cut(s)	186, 281, 539, 685, 756, 977, 2592, 3369
BseGI	GGATG	8 cut(s)	10, 850, 856, 862, 2101, 2404, 4114, 4152
BseLI	CCNNNNNNNGG	6 cut(s)	74, 366, 2078, 2395, 2581, 3971
BseMI	GCAATG	3 cut(s)	1728, 2247, 4089
BsePI	GCGCGC	1 cut(s)	4513
BseRI	GAGGAG	3 cut(s)	4, 502, 1478
BseSI	GKGCMC	3 cut(s)	325, 3345, 4309
BseYI	CCCAGC	3 cut(s)	315, 566, 3033
BsgI	GTGCAG	2 cut(s)	1644, 3414
Bsh1236I	CGCG	1 cut(s)	4515
BshNI	GGYRCC	1 cut(s)	1588
BshTI	ACCGGT	1 cut(s)	109
BshVI	ATCGAT	2 cut(s)	627, 3193
BsiHKAI	GWGCWC	6 cut(s)	299, 955, 2172, 2826, 3650, 4309
BsiHKCI	CYCGRG	2 cut(s)	2361, 3521
BsiSI	CCGG	6 cut(s)	42, 110, 2592, 3672, 4106, 4390
BslFI	GGGAC	3 cut(s)	476, 1822, 3675
BslI	CCNNNNNNNGG	6 cut(s)	74, 366, 2078, 2395, 2581, 3971
BsmAI	GTCTC	9 cut(s)	159, 245, 427, 975, 1947, 2304, 2426, 3148, 4321
BsmBI	CGTCTC	1 cut(s)	1947
BsmFI	GGGAC	3 cut(s)	476, 1822, 3675
BsmI	GAATGC	2 cut(s)	787, 2221
Bso31I	GGTCTC	2 cut(s)	159, 975
BsoBI	CYCGRG	2 cut(s)	2361, 3521
Bsp120I	GGGCCC	1 cut(s)	3341
Bsp1720I	GCTNAGC	2 cut(s)	268, 331
Bsp19I	CCATGG	3 cut(s)	281, 685, 756
BspDI	ATCGAT	2 cut(s)	627, 3193
BspFNI	CGCG	1 cut(s)	4515
BspHI	TCATGA	5 cut(s)	772, 2101, 3006, 3729, 4473
BspLI	GGNNCC	8 cut(s)	1048, 1590, 1810, 2163, 2379, 3343, 3474, 4460
BspMI	ACCTGC	1 cut(s)	2855
BspOI	GCTAGC	3 cut(s)	2006, 3272, 3378
BspPI	GGATC	4 cut(s)	1554, 1856, 3925, 3956
BspT107I	GGYRCC	1 cut(s)	1588
BspTI	CTTAAG	1 cut(s)	443
BspTNI	GGTCTC	2 cut(s)	159, 975
BsrBI	CCGCTC	3 cut(s)	99, 122, 330
BsrDI	GCAATG	3 cut(s)	1728, 2247, 4089
BsrFI	RCCGGY	3 cut(s)	109, 3671, 4389
BssAI	RCCGGY	3 cut(s)	109, 3671, 4389
BssECI	CCNNGG	8 cut(s)	186, 281, 539, 685, 756, 977, 2592, 3369
BssHII	GCGCGC	1 cut(s)	4513
BssT1I	CCWWGG	6 cut(s)	281, 539, 685, 756, 977, 3369
Bst2UI	CCWGG	4 cut(s)	223, 311, 2686, 3102
Bst6I	CTCTTC	4 cut(s)	978, 1171, 2745, 3008
BstAFI	CTTAAG	1 cut(s)	443
BstAPI	GCANNNNNTGC	3 cut(s)	1577, 2240, 4304
BstBAI	YACGTR	1 cut(s)	780
BstDSI	CCRYGG	3 cut(s)	281, 685, 756
BstEII	GGTNACC	2 cut(s)	2564, 4100
BstENI	CCTNNNNNAGG	2 cut(s)	72, 2076
BstF5I	GGATG	8 cut(s)	10, 850, 856, 862, 2101, 2404, 4114, 4152
BstFNI	CGCG	1 cut(s)	4515
BstHHI	GCGC	2 cut(s)	4515, 4517
BstMAI	GTCTC	9 cut(s)	159, 245, 427, 975, 1947, 2304, 2426, 3148, 4321
BstNI	CCWGG	4 cut(s)	223, 311, 2686, 3102
BstNSI	RCATGY	3 cut(s)	202, 2860, 2864
BstPAI	GACNNNNGTC	1 cut(s)	2438
BstPI	GGTNACC	2 cut(s)	2564, 4100
BstSCI	CCNGG	6 cut(s)	221, 309, 2591, 2684, 3100, 4104
BstSFI	CTRYAG	3 cut(s)	1491, 1867, 2436
BstSLI	GKGCMC	3 cut(s)	325, 3345, 4309
BstUI	CGCG	1 cut(s)	4515
BstV2I	GAAGAC	1 cut(s)	1381
BstX2I	RGATCY	3 cut(s)	216, 1157, 1861
BstXI	CCANNNNNNTGG	2 cut(s)	70, 692
BstYI	RGATCY	3 cut(s)	216, 1157, 1861
Bsu15I	ATCGAT	2 cut(s)	627, 3193
Bsu36I	CCTNAGG	2 cut(s)	2467, 3843
BsuI	GTATCC	1 cut(s)	1337
BsuTUI	ATCGAT	2 cut(s)	627, 3193
BtgI	CCRYGG	3 cut(s)	281, 685, 756
BtgZI	GCGATG	1 cut(s)	2381
BtrI	CACGTC	1 cut(s)	300
BtsCI	GGATG	8 cut(s)	10, 850, 856, 862, 2101, 2404, 4114, 4152
BtsI	GCAGTG	2 cut(s)	595, 3760
BveI	ACCTGC	1 cut(s)	2855
CciI	TCATGA	5 cut(s)	772, 2101, 3006, 3729, 4473
CfoI	GCGC	2 cut(s)	4515, 4517
Cfr10I	RCCGGY	3 cut(s)	109, 3671, 4389
ClaI	ATCGAT	2 cut(s)	627, 3193
CseI	GACGC	2 cut(s)	2008, 4349
CsiI	ACCWGGT	1 cut(s)	3100
CspAI	ACCGGT	1 cut(s)	109
DraI	TTTAAA	1 cut(s)	1918
DrdI	GACNNNNNNGTC	1 cut(s)	687
DseDI	GACNNNNNNGTC	1 cut(s)	687
EaeI	YGGCCR	3 cut(s)	43, 712, 782
Eam1104I	CTCTTC	4 cut(s)	978, 1171, 2745, 3008
EarI	CTCTTC	4 cut(s)	978, 1171, 2745, 3008
Ecl136II	GAGCTC	2 cut(s)	2824, 3648
Eco130I	CCWWGG	6 cut(s)	281, 539, 685, 756, 977, 3369
Eco147I	AGGCCT	2 cut(s)	2818, 3841
Eco24I	GRGCYC	5 cut(s)	269, 281, 2826, 3345, 3650
Eco31I	GGTCTC	2 cut(s)	159, 975
Eco32I	GATATC	1 cut(s)	3727
Eco47I	GGWCC	3 cut(s)	1809, 2377, 2761
Eco53kI	GAGCTC	2 cut(s)	2824, 3648
Eco57I	CTGAAG	3 cut(s)	1971, 2102, 4286
Eco72I	CACGTG	1 cut(s)	780
Eco81I	CCTNAGG	2 cut(s)	2467, 3843
Eco88I	CYCGRG	2 cut(s)	2361, 3521
Eco91I	GGTNACC	2 cut(s)	2564, 4100
EcoICRI	GAGCTC	2 cut(s)	2824, 3648
EcoNI	CCTNNNNNAGG	2 cut(s)	72, 2076
EcoO109I	RGGNCCY	3 cut(s)	1046, 2377, 3341
EcoO65I	GGTNACC	2 cut(s)	2564, 4100
EcoRII	CCWGG	4 cut(s)	221, 309, 2684, 3100
EcoRV	GATATC	1 cut(s)	3727
EcoT14I	CCWWGG	6 cut(s)	281, 539, 685, 756, 977, 3369
EcoT22I	ATGCAT	3 cut(s)	1254, 2862, 4537
EcoT38I	GRGCYC	5 cut(s)	269, 281, 2826, 3345, 3650
ErhI	CCWWGG	6 cut(s)	281, 539, 685, 756, 977, 3369
Esp3I	CGTCTC	1 cut(s)	1947
FaqI	GGGAC	3 cut(s)	476, 1822, 3675
FauI	CCCGC	2 cut(s)	104, 359
FauNDI	CATATG	2 cut(s)	1337, 3938
FbaI	TGATCA	6 cut(s)	706, 739, 823, 2370, 3327, 3444
FblI	GTMKAC	1 cut(s)	2036
FokI	GGATG	8 cut(s)	17, 857, 863, 869, 2108, 2411, 4121, 4139
FriOI	GRGCYC	5 cut(s)	269, 281, 2826, 3345, 3650
GlaI	GCGC	2 cut(s)	4514, 4516
GsaI	CCCAGC	3 cut(s)	319, 570, 3037
GsuI	CTGGAG	2 cut(s)	391, 1737
HapII	CCGG	6 cut(s)	42, 110, 2592, 3672, 4106, 4390
HgaI	GACGC	2 cut(s)	2008, 4349
HhaI	GCGC	2 cut(s)	4515, 4517
Hin6I	GCGC	2 cut(s)	4513, 4515
HinP1I	GCGC	2 cut(s)	4513, 4515
HincII	GTYRAC	2 cut(s)	1039, 1416
HindII	GTYRAC	2 cut(s)	1039, 1416
HindIII	AAGCTT	1 cut(s)	4146
HpaI	GTTAAC	1 cut(s)	1416
HpaII	CCGG	6 cut(s)	42, 110, 2592, 3672, 4106, 4390
Hpy166II	GTNNAC	6 cut(s)	680, 1039, 1416, 2037, 3619, 4307
Hpy8I	GTNNAC	6 cut(s)	680, 1039, 1416, 2037, 3619, 4307
Hpy99I	CGWCG	2 cut(s)	85, 241
HpyAV	CCTTC	8 cut(s)	144, 1342, 1755, 1899, 2399, 3080, 3970, 4023
HpyCH4IV	ACGT	9 cut(s)	55, 299, 453, 779, 1035, 2090, 2196, 3793, 3821
HpySE526I	ACGT	9 cut(s)	55, 299, 453, 779, 1035, 2090, 2196, 3793, 3821
HspAI	GCGC	2 cut(s)	4513, 4515
Ksp22I	TGATCA	6 cut(s)	706, 739, 823, 2370, 3327, 3444
KspAI	GTTAAC	1 cut(s)	1416
LweI	GCATC	5 cut(s)	850, 3349, 4099, 4123, 4452
MabI	ACCWGGT	1 cut(s)	3100
MaeII	ACGT	9 cut(s)	55, 299, 453, 779, 1035, 2090, 2196, 3793, 3821
MbiI	CCGCTC	3 cut(s)	99, 122, 330
MflI	RGATCY	3 cut(s)	216, 1157, 1861
MlsI	TGGCCA	1 cut(s)	714
MluNI	TGGCCA	1 cut(s)	714
MlyI	GAGTC	4 cut(s)	5, 86, 247, 3727
Mox20I	TGGCCA	1 cut(s)	714
Mph1103I	ATGCAT	3 cut(s)	1254, 2862, 4537
MscI	TGGCCA	1 cut(s)	714
MslI	CAYNNNNRTG	8 cut(s)	68, 690, 741, 771, 2292, 2865, 3692, 4488
Msp20I	TGGCCA	1 cut(s)	714
MspA1I	CMGCKG	2 cut(s)	566, 1484
MspCI	CTTAAG	1 cut(s)	443
MspI	CCGG	6 cut(s)	42, 110, 2592, 3672, 4106, 4390
MspR9I	CCNGG	6 cut(s)	223, 311, 2593, 2686, 3102, 4106
Mva1269I	GAATGC	2 cut(s)	787, 2221
MvaI	CCWGG	4 cut(s)	223, 311, 2686, 3102
MvnI	CGCG	1 cut(s)	4515
NciI	CCSGG	2 cut(s)	2593, 4106
NcoI	CCATGG	3 cut(s)	281, 685, 756
NdeI	CATATG	2 cut(s)	1337, 3938
NheI	GCTAGC	3 cut(s)	2002, 3268, 3374
NlaIV	GGNNCC	8 cut(s)	1048, 1590, 1810, 2163, 2379, 3343, 3474, 4460
NmeAIII	GCCGAG	1 cut(s)	1850
NmuCI	GTSAC	7 cut(s)	421, 750, 800, 1776, 2112, 2632, 3817
NsiI	ATGCAT	3 cut(s)	1254, 2862, 4537
NspI	RCATGY	3 cut(s)	202, 2860, 2864
OliI	CACNNNNGTG	1 cut(s)	68
PaeI	GCATGC	2 cut(s)	2860, 2864
PaeR7I	CTCGAG	1 cut(s)	2361
PagI	TCATGA	5 cut(s)	772, 2101, 3006, 3729, 4473
PaqCI	CACCTGC	1 cut(s)	2855
PauI	GCGCGC	1 cut(s)	4513
PceI	AGGCCT	2 cut(s)	2818, 3841
PciI	ACATGT	1 cut(s)	198
PcsI	WCGNNNNNNNCGW	1 cut(s)	1664
PctI	GAATGC	2 cut(s)	787, 2221
PfeI	GAWTC	9 cut(s)	1025, 1629, 2061, 2385, 2426, 2872, 2952, 3161, 4319
PflMI	CCANNNNNTGG	1 cut(s)	2581
PinAI	ACCGGT	1 cut(s)	109
PleI	GAGTC	4 cut(s)	5, 86, 247, 3727
PmaCI	CACGTG	1 cut(s)	780
PmlI	CACGTG	1 cut(s)	780
PpsI	GAGTC	4 cut(s)	5, 86, 247, 3727
Ppu21I	YACGTR	1 cut(s)	780
PpuMI	RGGWCCY	1 cut(s)	2377
PscI	ACATGT	1 cut(s)	198
PshAI	GACNNNNGTC	1 cut(s)	2438
PshBI	ATTAAT	1 cut(s)	1574
Psp124BI	GAGCTC	2 cut(s)	2826, 3650
Psp1406I	AACGTT	2 cut(s)	2090, 3793
Psp5II	RGGWCCY	1 cut(s)	2377
Psp6I	CCWGG	4 cut(s)	221, 309, 2684, 3100
PspCI	CACGTG	1 cut(s)	780
PspEI	GGTNACC	2 cut(s)	2564, 4100
PspFI	CCCAGC	3 cut(s)	315, 566, 3033
PspGI	CCWGG	4 cut(s)	221, 309, 2684, 3100
PspN4I	GGNNCC	8 cut(s)	1048, 1590, 1810, 2163, 2379, 3343, 3474, 4460
PspOMI	GGGCCC	1 cut(s)	3341
PspPPI	RGGWCCY	1 cut(s)	2377
PspXI	VCTCGAGB	1 cut(s)	2361
PsuI	RGATCY	3 cut(s)	216, 1157, 1861
PteI	GCGCGC	1 cut(s)	4513
PvuII	CAGCTG	2 cut(s)	566, 1484
RseI	CAYNNNNRTG	8 cut(s)	68, 690, 741, 771, 2292, 2865, 3692, 4488
SacI	GAGCTC	2 cut(s)	2826, 3650
ScaI	AGTACT	3 cut(s)	404, 905, 2973
SchI	GAGTC	4 cut(s)	5, 86, 247, 3727
ScrFI	CCNGG	6 cut(s)	223, 311, 2593, 2686, 3102, 4106
SexAI	ACCWGGT	1 cut(s)	3100
SfaNI	GCATC	5 cut(s)	850, 3349, 4099, 4123, 4452
SfcI	CTRYAG	3 cut(s)	1491, 1867, 2436
Sfr274I	CTCGAG	1 cut(s)	2361
SinI	GGWCC	3 cut(s)	1809, 2377, 2761
SlaI	CTCGAG	1 cut(s)	2361
SmiMI	CAYNNNNRTG	8 cut(s)	68, 690, 741, 771, 2292, 2865, 3692, 4488
SpeI	ACTAGT	1 cut(s)	797
SphI	GCATGC	2 cut(s)	2860, 2864
SseBI	AGGCCT	2 cut(s)	2818, 3841
SspI	AATATT	3 cut(s)	392, 1565, 3382
SstI	GAGCTC	2 cut(s)	2826, 3650
StuI	AGGCCT	2 cut(s)	2818, 3841
StyD4I	CCNGG	6 cut(s)	221, 309, 2591, 2684, 3100, 4104
StyI	CCWWGG	6 cut(s)	281, 539, 685, 756, 977, 3369
TaiI	ACGT	9 cut(s)	58, 302, 456, 782, 1038, 2093, 2199, 3796, 3824
TaqI	TCGA	8 cut(s)	21, 627, 1497, 1658, 1900, 2362, 2737, 3193
TaqII	GACCGA	1 cut(s)	440
TatI	WGTACW	3 cut(s)	402, 903, 2971
TauI	GCSGC	1 cut(s)	787
TfiI	GAWTC	9 cut(s)	1025, 1629, 2061, 2385, 2426, 2872, 2952, 3161, 4319
TseFI	GTSAC	7 cut(s)	421, 750, 800, 1776, 2112, 2632, 3817
Tsp45I	GTSAC	7 cut(s)	421, 750, 800, 1776, 2112, 2632, 3817
TspGWI	ACGGA	2 cut(s)	3068, 3561
Van91I	CCANNNNNTGG	1 cut(s)	2581
Vha464I	CTTAAG	1 cut(s)	443
VneI	GTGCAC	1 cut(s)	4305
VpaK11BI	GGWCC	3 cut(s)	1809, 2377, 2761
VspI	ATTAAT	1 cut(s)	1574
XagI	CCTNNNNNAGG	2 cut(s)	72, 2076
XapI	RAATTY	5 cut(s)	577, 1257, 1913, 2460, 3863
XbaI	TCTAGA	1 cut(s)	4288
XceI	RCATGY	3 cut(s)	202, 2860, 2864
XhoI	CTCGAG	1 cut(s)	2361
XmiI	GTMKAC	1 cut(s)	2036
ZrmI	AGTACT	3 cut(s)	404, 905, 2973
Zsp2I	ATGCAT	3 cut(s)	1254, 2862, 4537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.