Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0017342

Physical Location & Seq

Genomic Coordinates Reverse (-)

60755090 .. 60760679

Gene Structure 5590 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G036930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4506 bp

ATGGATGCAGACTCCTCATTCGAGCTGGAAAACTCTATTTCCGGCCACCTTCCACGTTACACCACCCCTGTGGCAGGCTCGTCGCATTATGACTCCCGCTCATTCACACCGGTTAGCTCCGCTCACTCGAGAACTCCCAGAAGGTCTCGCCACCGGACTCCAACTACGCCTTTCGCCTCGGACAATGACATGTCGTGGCAAGGGGAGATCTCCTGGCAGTTTGAGCCGACGGGCTGGAACGAAACTCGCAACTTGGGCTCAGCTATGAGCCCGTGGGCCACCGTGAGCACGTCCTCTCGCCAGGCCCAGCGTGCCCACCGCTCAGCAAATTACTACTACCTCTCCCGCACCACTGGCGGGTTTCGTACCTCCCCAAATCAATATTACAACGAGTACTCCAGCTATGGTGCTGTGACAGATGGGAGACTTGAGCTTAAGAGCTACGTTGCTAGGGACAATGAAAGCTCAACTTTTGGGAGGAGTAGTTACAATTATGGGGAGCAGAAGAAACCCTTTAAGGCAGTTACTCCAAGGTTGGAGGGTATTAAAGAAGCAGCTGGGAGTGGAAATTTCAGTGGCAGTGGCCCTTTGTCTAAGAAAGATTTGCTTGGTTTGATCGATTATCAAACAGCAGAAGATGATGATGAAGATGAGAGTTTGGGTCATAGTGGACACCATGGTCATGGCCTACATCTTGATCATGGTCATAGGCCATCACACAAAGGACATGATCATAGGTTGTCACCCATGGGTCATCATGGTCATGACACGTGGCCGCATTCAATTACTAGTCACGATATTGATGATGAGAGTGATCAGGGTTCTGGGTATGATGATGAGGATGAGGATGAGGATGAGGATGATGCAAGGCCAGTGAAGCAAGTTGGGTTGTTTAGCTTGTTCAAGTACTCAACAAAATGGGATATGTTTCTTGTGTTTTTGGGTTGTGTTGGTGCTCTTATCAATGGAGGGTCTCTTCCTTGGTATTCTTATCTCTTTGGAAAGTTTGTGAATAAGATTGCTAAAGAATCAAAGGACGTTGACAAAGGCCCCATGATGAGAGATGTAGAGAAGATATGCCTGCTTATGACTGGTTTAGCTGCAATAGTAGTAGTTGGAGCATATATGGATCACTTGTTGGAGAATGGTGGGGGAAGATCTGCTCAGAGAATAAGAAGAGAATATCTAAAAGCAGTTCTGCGACAAGACATTAGCTTTTTTGATACTGAGATTGCTGCTGGTGATATTATGCATGGAATTTCAAGTGATGTTGCTGCAATCCAAGAAGTCATGGGGGAGAAGATGGCACATTTCATTCACCATATATGTACCTTCATATGTGGATACACAGTTGGGTTTCTAAGGTCATGGAAAGTAACTCTAGTAGTCTTCTCAGTCATACCATTGATGATGTTAACTGGTATCGCTTACAAGGCTATTTATGTTGGCTTAACCACAAAAGAGGAGTTTTCTTACAGGACAGCTGGTACTGTAGTCGAGCAAGCTATCAGTTCAATCAGAACAGTATTTTCCTTTGTTGCGGAGGATCATTTGGCTGAAAAATATTCTGCATTAATTGCCAACTTGGTGCCTTTTGGAGCAAAGATTGGCTTTGCTAAGGGTGCAGGAGTCGGAGTTATCTATCTTGTTAGTTATTCTACATGGGCGTTGGCTTTCTGGTACGGTTCTCTTTTGGTTGCTAAGGGTGAGATCACTGGAGGTGCTGCCATTGCTTGTTTCTTTGGTGTAAATGTCGGGGGAAGGGGCTTGGCATTGTCACTGTCATACTTTGCTCAGTTTGCACAAGGGACCGTAGCAGCAGGCCGAGTATTTGAAATTATAGATAGAGTTCCAGAAATAGATCCCTACAGCCCAGTAGGTAGGAAACTGTCAAATGTTCGAGGAAGGATTGAATTTAAAGGCATTTATTTCGCATACCCGTCTCGTCCTGAAGCTCCAATTTTTCATTCGCTTAATCTGGTCATTCCATCTTCAAAGACGCTAGCACTTGTTGGCTCTAGCGGAGGTGGAAAGTCTACGATCTTTGCTCTTATAGAGAGATTCTATGACCCTACCAAAGGTACAATAACATTGGATGGTCATGATTTAAGGTCACTTCAGGAACCAGTGCTCTTTGCCACCAGCATTTTAGAAAACGTGTTGATGGGGAAAGAGAATGCCACCAAGAAAGAAGCAATTACTGCCTGCATTGCTGCCAATGCTCACAGCTTCATTTCTGGACTTCCGCATGGCTATGACACTCAGGTTGGAGACAGAGGAACACTACTCTCAGGTGGTCAGAAACAAAGAATTGCGTTGGCTCGAGCGATGATCAAGGACCCTAGAATCCTTCTCTTGGATGAACCTACCAGTGCCTTAGACCCTGAATCCGAGACTGTAGTCCAACAGGCTATTGACAAAATTTCCTCAGGACGAACAACTATTGTAATTGCTCACAGGCTATCAACTGTAAGAAACTCTCATGCCATTGTTGTTCTTGACAGTGGCTCTGTCATTGAGATTGGTAACCACCGCCAGCTCATGGAAAAAGCTGGGGCGTATTATAGCCTCGTTGAGCTTGCTTCTGCTGGAGTGACAAAACCTTTTTCGGAACAATATGATACTGGAATTAGCCCTCAGTTTTCCAGGTTTGACAAATCAGCTTATGATGTATCAAGATCAACATTCATGCAAGATCGAAACCAAGTAGAAGAGAAAGAGGTCCAAAAACCAATTCCAAGAAAGGTTCAGCTTTCAGATATATGGTTGTTACAGAGGCCTGAGCTCCCAATGCTATTAGTTGGATTTCTTTTGGGCATGCATGCAGGTGCGATTCTATCTATCTTCCCTTATATTCTAGGCCAAGCACTTGTAGTTTATTTTGATAAAGAGCCATCAAAGATCAAAACAGGAATCGCGCCCCTTTGCTTAGTACTTGTTGCACTTGGCTTTGGGAATATACTCTTCATGACAGGACAACAAGGCTTGTGTGGTTGGGCTGGAACAAAGCTCACAATGAGAGTGAGAAACCTTCTTTTCCGTTCCATACTTAAACAAGAACCTGGTTGGTTTGATTTTGAAGAAAACTCAAAAGCGGTACTTGTCTCTAGGATTTCAATGGATTCTGTGGCTTTTCGTGCCGTCCATGTTGATCGATTATCAGTCTTGTTTATGGGGTTGAGTTCAGCTATGGTTGGATTGGGTATATCCTTTTGGCTTGAATGGAGGCTAGCTCTTTTGGCTGCTGCTCTCACTCCTTTCACTCTTGGTGCAAGTTACTTGAGCTTGATCATAAATATAGGGCCCAAACTTGACAATGATGCTTATGCCAAGGCTAGCAATATTGCTTCTGGTGCAGTTTCAAACATAAGGACGGTTGCAACATTTTCTGCTCAAGACCAGTTGATCAAGTCCTTTGATAGTGCTTTATCGGAGCCAAAGACAAAATCAATGAGAAGAACACAAATAATGGGCCTAGCACTCGGGTTCTCTCAAGGTGTTATGTACGGAGCTTACACCTTAACTCTCTTGTTTGGTGCTTACCTTATGAAAGAAAACAAAACTAATTTCGGTGATGTTTACAAAATCTTTCTCATTCTTGTTCTGAGCTCATTTTCTGTGGGACAATTAGCCGGTCTTGCACCAGACACATCTATGGCTGAAACAGCAATTCCAGCAGTTTTTGATATCATGACTCGTAAGCCTTTAATCAGCAGTGGGAGAGGAAAGGATAAGAAGATTGAAAGGTCGAAACGTTTAGATATTCACTTCAAAATGGTGACGTTTGCATATCCATCAAGGCCTGAGGTAACTGTGTTGAGTGAATTTTCCCTCAAGGTCAAAGGTGGTAGCACAGTGGCATTGGTGGGTGGAAGTGGATCGGGAAAATCAACTGTCATATGGTTAATACAAAGGTTTTATGATCCAACTCAAGGGAAGGTGATGATGGGGGGAGTGGATTTGAGGGAGATTGATGTGAAGTGGTTAAGAAGGCAAACAGCTTTAGTTGGTCAAGAGCCTACATTGTTTGCTGGCACTATAAGAGAGAACATTGCTTTTGGTAACCCGGATGCTTCATGGACTGAAATTGAAGATGCTGCAAGAGAAGCTTACATCCACAGCTTCATTAGTGGCCTCCCTCAAGGCTATGAAACTCAGGTTGGTGAGAGTGGGGTTCAGCTATCTGGGGGGCAGAAGCAAAGGATTGCCATAGCTAGAGCTATACTGAAGAAATCAAAGGTACTACTTCTAGATGAAGCAAGTAGTGCACTAGATTTGGAATCTGAGAGACATATACAAGACGCACTTAGGAAAGTCAGCAAAAGGGCCACGACAATCATAGTCGCTCACCGGCTTTCTACTATCAGGGAAGCTGATATGATAGCAGTTATGAAAGATGGTGGCATCACTGAATATGGGAGCCATGATACGCTCATGAAATCTCATCTTAATGGCGTGTATGCCAGCTTGGTGCGTGCAGAAACCGAAGCCAATGCATTTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009966 GO:0009987 GO:0010033 GO:0010218 GO:0010315 GO:0010329 GO:0010540 GO:0010541 GO:0010646 GO:0010817 GO:0010928 GO:0015562 GO:0016020 GO:0016043 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0032501 GO:0032502 GO:0032535 GO:0042221 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044464 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048527 GO:0048528 GO:0048583 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0048859 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080161 GO:0090066 GO:0090567 GO:0090691 GO:0090696 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1501

Amino Acids

164.49

Weight (kDa)

7.48

Isoelectric Point (pI)

36.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	312 - 584	3.9e-52	ABC transporter transmembrane region
ABC_tran	PF00005	656 - 791	6.4e-25	ABC transporter
SMC_N	PF02463	888 - 1464	4e-07	RecF/RecN/SMC N terminal domain
ABC_membrane	PF00664	932 - 1203	1.7e-43	ABC transporter transmembrane region
ABC_tran	PF00005	1274 - 1421	2.2e-30	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017342)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35260
malus_domestica	MD09G1177100.v1.1 MD17G1149200.v1.1
prunus_persica	Prupe.3G036400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0146621
rosa_laevigata	RLG00000020211
rosa_multiflora	Rmu_sc0001986.1_g000032
rosa_roxburghii	Rroxscaffold_2G00100000
rosa_rugosa	Rorug02G0395800 Rorug02G0395800
rosa_samantha	Rh2BG464800 Rh2DG473800
rosa_wichuraiana	Rw2G036930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2816
AasI	GACNNNNNNGTC	1 cut(s)	678
Acc36I	ACCTGC	1 cut(s)	2816
AccB1I	GGYRCC	1 cut(s)	1588
AccB7I	CCANNNNNTGG	1 cut(s)	2542
AccBSI	CCGCTC	3 cut(s)	99, 122, 321
AccI	GTMKAC	1 cut(s)	2036
AccII	CGCG	1 cut(s)	2918
AclI	AACGTT	1 cut(s)	3754
AclWI	GGATC	5 cut(s)	1137, 1554, 1856, 3886, 3917
AcoI	YGGCCR	2 cut(s)	43, 773
AcsI	RAATTY	5 cut(s)	568, 1257, 1913, 2421, 3824
AcuI	CTGAAG	3 cut(s)	1971, 2102, 4247
AcvI	CACGTG	1 cut(s)	771
AfiI	CCNNNNNNNGG	6 cut(s)	74, 357, 2078, 2356, 2542, 3932
AflII	CTTAAG	1 cut(s)	434
AflIII	ACRYGT	3 cut(s)	189, 768, 2157
AgeI	ACCGGT	1 cut(s)	109
AhlI	ACTAGT	1 cut(s)	788
AjiI	CACGTC	1 cut(s)	291
AjnI	CCWGG	4 cut(s)	212, 300, 2645, 3061
AjuI	GAANNNNNNNTTGG	6 cut(s)	1951, 1983, 2718, 2750, 4143, 4175
AleI	CACNNNNGTG	1 cut(s)	68
Alw21I	GWGCWC	6 cut(s)	290, 958, 2133, 2787, 3611, 4270
Alw26I	GTCTC	8 cut(s)	150, 418, 978, 1947, 2265, 2387, 3109, 4282
Alw44I	GTGCAC	1 cut(s)	4266
AlwI	GGATC	5 cut(s)	1137, 1554, 1856, 3886, 3917
Ama87I	CYCGRG	3 cut(s)	127, 2322, 3482
ApaI	GGGCCC	1 cut(s)	3306
ApaLI	GTGCAC	1 cut(s)	4266
ApoI	RAATTY	5 cut(s)	568, 1257, 1913, 2421, 3824
ArsI	GACNNNNNNTTYG	1 cut(s)	34
AseI	ATTAAT	1 cut(s)	1574
AsiGI	ACCGGT	1 cut(s)	109
AspLEI	GCGC	1 cut(s)	2920
AsuC2I	CCSGG	1 cut(s)	4067
AsuHPI	GGTGA	9 cut(s)	735, 1253, 1310, 1718, 3584, 3790, 3952, 4175, 4340
AsuNHI	GCTAGC	3 cut(s)	2002, 3229, 3335
AvaI	CYCGRG	3 cut(s)	127, 2322, 3482
AvaII	GGWCC	3 cut(s)	1809, 2338, 2722
AxyI	CCTNAGG	2 cut(s)	2428, 3804
BaeGI	GKGCMC	3 cut(s)	316, 3306, 4270
BaeI	ACNNNNGTAYC	2 cut(s)	1479, 1512
BanI	GGYRCC	1 cut(s)	1588
BanII	GRGCYC	5 cut(s)	260, 272, 2787, 3306, 3611
BbrPI	CACGTG	1 cut(s)	771
BbsI	GAAGAC	1 cut(s)	1381
Bbv12I	GWGCWC	6 cut(s)	290, 958, 2133, 2787, 3611, 4270
BceAI	ACGGC	1 cut(s)	3125
BcgI	CGANNNNNNTGC	5 cut(s)	63, 97, 1912, 1946, 4475
BciT130I	CCWGG	4 cut(s)	214, 302, 2647, 3063
BciVI	GTATCC	1 cut(s)	1337
BclI	TGATCA	6 cut(s)	697, 730, 814, 2331, 3288, 3405
BcnI	CCSGG	1 cut(s)	4067
BcoDI	GTCTC	8 cut(s)	150, 418, 978, 1947, 2265, 2387, 3109, 4282
BcuI	ACTAGT	1 cut(s)	788
BfmI	CTRYAG	3 cut(s)	1491, 1867, 2397
BfrI	CTTAAG	1 cut(s)	434
BfuAI	ACCTGC	1 cut(s)	2816
BfuI	GTATCC	1 cut(s)	1337
BglII	AGATCT	2 cut(s)	207, 1157
BlpI	GCTNAGC	2 cut(s)	259, 322
BmcAI	AGTACT	3 cut(s)	395, 908, 2934
Bme1390I	CCNGG	5 cut(s)	214, 302, 2647, 3063, 4067
Bme18I	GGWCC	3 cut(s)	1809, 2338, 2722
BmeT110I	CYCGRG	3 cut(s)	127, 2322, 3482
BmgBI	CACGTC	1 cut(s)	291
BmiI	GGNNCC	8 cut(s)	1051, 1590, 1810, 2124, 2340, 3304, 3435, 4421
BmrFI	CCNGG	5 cut(s)	214, 302, 2647, 3063, 4067
BmrI	ACTGGG	1 cut(s)	1868
BmsI	GCATC	5 cut(s)	853, 3310, 4060, 4084, 4413
BmtI	GCTAGC	3 cut(s)	2006, 3233, 3339
BmuI	ACTGGG	1 cut(s)	1868
BoxI	GACNNNNGTC	1 cut(s)	2399
BpiI	GAAGAC	1 cut(s)	1381
BplI	GAGNNNNNCTC	2 cut(s)	3217, 3249
BpmI	CTGGAG	3 cut(s)	382, 1737, 2610
Bpu10I	CCTNAGC	3 cut(s)	1617, 1701, 2781
Bpu1102I	GCTNAGC	2 cut(s)	259, 322
BpuEI	CTTGAG	7 cut(s)	449, 3301, 3378, 3477, 3818, 3915, 4125
BpuMI	CCSGG	1 cut(s)	4067
Bsa29I	ATCGAT	2 cut(s)	618, 3154
BsaAI	YACGTR	1 cut(s)	771
BsaI	GGTCTC	2 cut(s)	150, 978
BsaJI	CCNNGG	7 cut(s)	177, 272, 530, 676, 747, 980, 3330
BsaWI	WCCGGW	2 cut(s)	109, 153
BsaXI	ACNNNNNCTCC	8 cut(s)	326, 356, 380, 410, 469, 499, 1620, 1650
Bsc4I	CCNNNNNNNGG	6 cut(s)	74, 357, 2078, 2356, 2542, 3932
Bse118I	RCCGGY	3 cut(s)	109, 3632, 4350
Bse21I	CCTNAGG	2 cut(s)	2428, 3804
Bse3DI	GCAATG	3 cut(s)	1728, 2208, 4050
BseBI	CCWGG	4 cut(s)	214, 302, 2647, 3063
BseCI	ATCGAT	2 cut(s)	618, 3154
BseDI	CCNNGG	7 cut(s)	177, 272, 530, 676, 747, 980, 3330
BseGI	GGATG	9 cut(s)	10, 847, 853, 859, 865, 2101, 2365, 4075, 4113
BseLI	CCNNNNNNNGG	6 cut(s)	74, 357, 2078, 2356, 2542, 3932
BseMI	GCAATG	3 cut(s)	1728, 2208, 4050
BseRI	GAGGAG	3 cut(s)	4, 493, 1478
BseSI	GKGCMC	3 cut(s)	316, 3306, 4270
BseYI	CCCAGC	3 cut(s)	306, 557, 2552
BsgI	GTGCAG	3 cut(s)	1644, 3375, 4497
Bsh1236I	CGCG	1 cut(s)	2918
BshNI	GGYRCC	1 cut(s)	1588
BshTI	ACCGGT	1 cut(s)	109
BshVI	ATCGAT	2 cut(s)	618, 3154
BsiHKAI	GWGCWC	6 cut(s)	290, 958, 2133, 2787, 3611, 4270
BsiHKCI	CYCGRG	3 cut(s)	127, 2322, 3482
BsiSI	CCGG	6 cut(s)	42, 110, 154, 3633, 4067, 4351
BslFI	GGGAC	3 cut(s)	467, 1822, 3636
BslI	CCNNNNNNNGG	6 cut(s)	74, 357, 2078, 2356, 2542, 3932
BsmAI	GTCTC	8 cut(s)	150, 418, 978, 1947, 2265, 2387, 3109, 4282
BsmBI	CGTCTC	1 cut(s)	1947
BsmFI	GGGAC	3 cut(s)	467, 1822, 3636
BsmI	GAATGC	2 cut(s)	778, 2182
Bso31I	GGTCTC	2 cut(s)	150, 978
BsoBI	CYCGRG	3 cut(s)	127, 2322, 3482
Bsp120I	GGGCCC	1 cut(s)	3302
Bsp1720I	GCTNAGC	2 cut(s)	259, 322
Bsp19I	CCATGG	2 cut(s)	676, 747
BspDI	ATCGAT	2 cut(s)	618, 3154
BspFNI	CGCG	1 cut(s)	2918
BspHI	TCATGA	5 cut(s)	763, 2101, 2967, 3690, 4434
BspLI	GGNNCC	8 cut(s)	1051, 1590, 1810, 2124, 2340, 3304, 3435, 4421
BspMI	ACCTGC	1 cut(s)	2816
BspOI	GCTAGC	3 cut(s)	2006, 3233, 3339
BspPI	GGATC	5 cut(s)	1137, 1554, 1856, 3886, 3917
BspT107I	GGYRCC	1 cut(s)	1588
BspTI	CTTAAG	1 cut(s)	434
BspTNI	GGTCTC	2 cut(s)	150, 978
BsrBI	CCGCTC	3 cut(s)	99, 122, 321
BsrDI	GCAATG	3 cut(s)	1728, 2208, 4050
BsrFI	RCCGGY	3 cut(s)	109, 3632, 4350
BssAI	RCCGGY	3 cut(s)	109, 3632, 4350
BssECI	CCNNGG	7 cut(s)	177, 272, 530, 676, 747, 980, 3330
BssT1I	CCWWGG	5 cut(s)	530, 676, 747, 980, 3330
Bst2UI	CCWGG	4 cut(s)	214, 302, 2647, 3063
Bst6I	CTCTTC	4 cut(s)	981, 1171, 2706, 2969
BstAFI	CTTAAG	1 cut(s)	434
BstAPI	GCANNNNNTGC	3 cut(s)	1577, 2201, 4265
BstBAI	YACGTR	1 cut(s)	771
BstDSI	CCRYGG	3 cut(s)	272, 676, 747
BstEII	GGTNACC	2 cut(s)	2525, 4061
BstENI	CCTNNNNNAGG	2 cut(s)	72, 2076
BstF5I	GGATG	9 cut(s)	10, 847, 853, 859, 865, 2101, 2365, 4075, 4113
BstFNI	CGCG	1 cut(s)	2918
BstHHI	GCGC	1 cut(s)	2920
BstMAI	GTCTC	8 cut(s)	150, 418, 978, 1947, 2265, 2387, 3109, 4282
BstNI	CCWGG	4 cut(s)	214, 302, 2647, 3063
BstNSI	RCATGY	3 cut(s)	193, 2821, 2825
BstPAI	GACNNNNGTC	1 cut(s)	2399
BstPI	GGTNACC	2 cut(s)	2525, 4061
BstSCI	CCNGG	5 cut(s)	212, 300, 2645, 3061, 4065
BstSFI	CTRYAG	3 cut(s)	1491, 1867, 2397
BstSLI	GKGCMC	3 cut(s)	316, 3306, 4270
BstUI	CGCG	1 cut(s)	2918
BstV2I	GAAGAC	1 cut(s)	1381
BstX2I	RGATCY	3 cut(s)	207, 1157, 1861
BstXI	CCANNNNNNTGG	2 cut(s)	70, 683
BstYI	RGATCY	3 cut(s)	207, 1157, 1861
Bsu15I	ATCGAT	2 cut(s)	618, 3154
Bsu36I	CCTNAGG	2 cut(s)	2428, 3804
BsuI	GTATCC	1 cut(s)	1337
BsuTUI	ATCGAT	2 cut(s)	618, 3154
BtgI	CCRYGG	3 cut(s)	272, 676, 747
BtgZI	GCGATG	1 cut(s)	2342
BtrI	CACGTC	1 cut(s)	291
BtsCI	GGATG	9 cut(s)	10, 847, 853, 859, 865, 2101, 2365, 4075, 4113
BtsI	GCAGTG	2 cut(s)	586, 3721
BveI	ACCTGC	1 cut(s)	2816
CciI	TCATGA	5 cut(s)	763, 2101, 2967, 3690, 4434
CfoI	GCGC	1 cut(s)	2920
Cfr10I	RCCGGY	3 cut(s)	109, 3632, 4350
ClaI	ATCGAT	2 cut(s)	618, 3154
CseI	GACGC	2 cut(s)	2008, 4310
CsiI	ACCWGGT	1 cut(s)	3061
CspAI	ACCGGT	1 cut(s)	109
DraI	TTTAAA	1 cut(s)	1918
DrdI	GACNNNNNNGTC	1 cut(s)	678
DseDI	GACNNNNNNGTC	1 cut(s)	678
EaeI	YGGCCR	2 cut(s)	43, 773
Eam1104I	CTCTTC	4 cut(s)	981, 1171, 2706, 2969
EarI	CTCTTC	4 cut(s)	981, 1171, 2706, 2969
Ecl136II	GAGCTC	2 cut(s)	2785, 3609
Eco130I	CCWWGG	5 cut(s)	530, 676, 747, 980, 3330
Eco147I	AGGCCT	2 cut(s)	2779, 3802
Eco24I	GRGCYC	5 cut(s)	260, 272, 2787, 3306, 3611
Eco31I	GGTCTC	2 cut(s)	150, 978
Eco32I	GATATC	1 cut(s)	3688
Eco47I	GGWCC	3 cut(s)	1809, 2338, 2722
Eco53kI	GAGCTC	2 cut(s)	2785, 3609
Eco57I	CTGAAG	3 cut(s)	1971, 2102, 4247
Eco72I	CACGTG	1 cut(s)	771
Eco81I	CCTNAGG	2 cut(s)	2428, 3804
Eco88I	CYCGRG	3 cut(s)	127, 2322, 3482
Eco91I	GGTNACC	2 cut(s)	2525, 4061
EcoICRI	GAGCTC	2 cut(s)	2785, 3609
EcoNI	CCTNNNNNAGG	2 cut(s)	72, 2076
EcoO109I	RGGNCCY	3 cut(s)	1049, 2338, 3302
EcoO65I	GGTNACC	2 cut(s)	2525, 4061
EcoRII	CCWGG	4 cut(s)	212, 300, 2645, 3061
EcoRV	GATATC	1 cut(s)	3688
EcoT14I	CCWWGG	5 cut(s)	530, 676, 747, 980, 3330
EcoT22I	ATGCAT	3 cut(s)	1254, 2823, 4498
EcoT38I	GRGCYC	5 cut(s)	260, 272, 2787, 3306, 3611
ErhI	CCWWGG	5 cut(s)	530, 676, 747, 980, 3330
Esp3I	CGTCTC	1 cut(s)	1947
FaqI	GGGAC	3 cut(s)	467, 1822, 3636
FauI	CCCGC	3 cut(s)	104, 350, 353
FauNDI	CATATG	2 cut(s)	1337, 3899
FbaI	TGATCA	6 cut(s)	697, 730, 814, 2331, 3288, 3405
FblI	GTMKAC	1 cut(s)	2036
FokI	GGATG	9 cut(s)	17, 854, 860, 866, 872, 2108, 2372, 4082, 4100
FriOI	GRGCYC	5 cut(s)	260, 272, 2787, 3306, 3611
GlaI	GCGC	1 cut(s)	2919
GsaI	CCCAGC	3 cut(s)	310, 561, 2556
GsuI	CTGGAG	3 cut(s)	382, 1737, 2610
HapII	CCGG	6 cut(s)	42, 110, 154, 3633, 4067, 4351
HgaI	GACGC	2 cut(s)	2008, 4310
HhaI	GCGC	1 cut(s)	2920
Hin6I	GCGC	1 cut(s)	2918
HinP1I	GCGC	1 cut(s)	2918
HincII	GTYRAC	2 cut(s)	1042, 1416
HindII	GTYRAC	2 cut(s)	1042, 1416
HindIII	AAGCTT	1 cut(s)	4107
HpaI	GTTAAC	1 cut(s)	1416
HpaII	CCGG	6 cut(s)	42, 110, 154, 3633, 4067, 4351
HphI	GGTGA	9 cut(s)	735, 1253, 1310, 1718, 3584, 3790, 3952, 4175, 4340
Hpy166II	GTNNAC	6 cut(s)	671, 1042, 1416, 2037, 3580, 4268
Hpy8I	GTNNAC	6 cut(s)	671, 1042, 1416, 2037, 3580, 4268
Hpy99I	CGWCG	2 cut(s)	85, 232
HpyAV	CCTTC	9 cut(s)	59, 135, 1342, 1755, 1899, 2360, 3041, 3931, 3984
HpyCH4IV	ACGT	8 cut(s)	55, 290, 444, 770, 1038, 2157, 3754, 3782
HpySE526I	ACGT	8 cut(s)	55, 290, 444, 770, 1038, 2157, 3754, 3782
HspAI	GCGC	1 cut(s)	2918
Ksp22I	TGATCA	6 cut(s)	697, 730, 814, 2331, 3288, 3405
KspAI	GTTAAC	1 cut(s)	1416
LmnI	GCTCC	9 cut(s)	122, 499, 1118, 1598, 1960, 2790, 3433, 3509, 4419
LweI	GCATC	5 cut(s)	853, 3310, 4060, 4084, 4413
MabI	ACCWGGT	1 cut(s)	3061
MaeII	ACGT	8 cut(s)	55, 290, 444, 770, 1038, 2157, 3754, 3782
MbiI	CCGCTC	3 cut(s)	99, 122, 321
MflI	RGATCY	3 cut(s)	207, 1157, 1861
MlyI	GAGTC	5 cut(s)	5, 86, 151, 1638, 3688
Mph1103I	ATGCAT	3 cut(s)	1254, 2823, 4498
MslI	CAYNNNNRTG	7 cut(s)	68, 681, 762, 2253, 2826, 3653, 4449
MspA1I	CMGCKG	2 cut(s)	557, 1484
MspCI	CTTAAG	1 cut(s)	434
MspI	CCGG	6 cut(s)	42, 110, 154, 3633, 4067, 4351
MspR9I	CCNGG	5 cut(s)	214, 302, 2647, 3063, 4067
Mva1269I	GAATGC	2 cut(s)	778, 2182
MvaI	CCWGG	4 cut(s)	214, 302, 2647, 3063
MvnI	CGCG	1 cut(s)	2918
NciI	CCSGG	1 cut(s)	4067
NcoI	CCATGG	2 cut(s)	676, 747
NdeI	CATATG	2 cut(s)	1337, 3899
NheI	GCTAGC	3 cut(s)	2002, 3229, 3335
NlaIV	GGNNCC	8 cut(s)	1051, 1590, 1810, 2124, 2340, 3304, 3435, 4421
NmeAIII	GCCGAG	1 cut(s)	1850
NmuCI	GTSAC	7 cut(s)	412, 741, 791, 1776, 2112, 2593, 3778
NsiI	ATGCAT	3 cut(s)	1254, 2823, 4498
NspI	RCATGY	3 cut(s)	193, 2821, 2825
OliI	CACNNNNGTG	1 cut(s)	68
PaeI	GCATGC	2 cut(s)	2821, 2825
PaeR7I	CTCGAG	2 cut(s)	127, 2322
PagI	TCATGA	5 cut(s)	763, 2101, 2967, 3690, 4434
PaqCI	CACCTGC	1 cut(s)	2816
PceI	AGGCCT	2 cut(s)	2779, 3802
PciI	ACATGT	1 cut(s)	189
PctI	GAATGC	2 cut(s)	778, 2182
PfeI	GAWTC	8 cut(s)	1028, 2061, 2346, 2387, 2833, 2913, 3122, 4280
PflMI	CCANNNNNTGG	1 cut(s)	2542
PinAI	ACCGGT	1 cut(s)	109
PleI	GAGTC	5 cut(s)	5, 86, 151, 1637, 3688
PmaCI	CACGTG	1 cut(s)	771
PmlI	CACGTG	1 cut(s)	771
PpsI	GAGTC	5 cut(s)	5, 86, 151, 1637, 3688
Ppu21I	YACGTR	1 cut(s)	771
PpuMI	RGGWCCY	1 cut(s)	2338
PscI	ACATGT	1 cut(s)	189
PshAI	GACNNNNGTC	1 cut(s)	2399
PshBI	ATTAAT	1 cut(s)	1574
Psp124BI	GAGCTC	2 cut(s)	2787, 3611
Psp1406I	AACGTT	1 cut(s)	3754
Psp5II	RGGWCCY	1 cut(s)	2338
Psp6I	CCWGG	4 cut(s)	212, 300, 2645, 3061
PspCI	CACGTG	1 cut(s)	771
PspEI	GGTNACC	2 cut(s)	2525, 4061
PspFI	CCCAGC	3 cut(s)	306, 557, 2552
PspGI	CCWGG	4 cut(s)	212, 300, 2645, 3061
PspN4I	GGNNCC	8 cut(s)	1051, 1590, 1810, 2124, 2340, 3304, 3435, 4421
PspOMI	GGGCCC	1 cut(s)	3302
PspPPI	RGGWCCY	1 cut(s)	2338
PspXI	VCTCGAGB	1 cut(s)	2322
PsuI	RGATCY	3 cut(s)	207, 1157, 1861
PvuII	CAGCTG	2 cut(s)	557, 1484
RseI	CAYNNNNRTG	7 cut(s)	68, 681, 762, 2253, 2826, 3653, 4449
SacI	GAGCTC	2 cut(s)	2787, 3611
ScaI	AGTACT	3 cut(s)	395, 908, 2934
SchI	GAGTC	5 cut(s)	5, 86, 151, 1638, 3688
ScrFI	CCNGG	5 cut(s)	214, 302, 2647, 3063, 4067
SexAI	ACCWGGT	1 cut(s)	3061
SfaNI	GCATC	5 cut(s)	853, 3310, 4060, 4084, 4413
SfcI	CTRYAG	3 cut(s)	1491, 1867, 2397
Sfr274I	CTCGAG	2 cut(s)	127, 2322
SinI	GGWCC	3 cut(s)	1809, 2338, 2722
SlaI	CTCGAG	2 cut(s)	127, 2322
SmiMI	CAYNNNNRTG	7 cut(s)	68, 681, 762, 2253, 2826, 3653, 4449
SpeI	ACTAGT	1 cut(s)	788
SphI	GCATGC	2 cut(s)	2821, 2825
SseBI	AGGCCT	2 cut(s)	2779, 3802
SspI	AATATT	3 cut(s)	383, 1565, 3343
SstI	GAGCTC	2 cut(s)	2787, 3611
StuI	AGGCCT	2 cut(s)	2779, 3802
StyD4I	CCNGG	5 cut(s)	212, 300, 2645, 3061, 4065
StyI	CCWWGG	5 cut(s)	530, 676, 747, 980, 3330
TaiI	ACGT	8 cut(s)	58, 293, 447, 773, 1041, 2160, 3757, 3785
TaqI	TCGA	9 cut(s)	21, 128, 618, 1497, 1900, 2323, 2698, 3154, 3749
TatI	WGTACW	3 cut(s)	393, 906, 2932
TauI	GCSGC	1 cut(s)	778
TfiI	GAWTC	8 cut(s)	1028, 2061, 2346, 2387, 2833, 2913, 3122, 4280
TseFI	GTSAC	7 cut(s)	412, 741, 791, 1776, 2112, 2593, 3778
Tsp45I	GTSAC	7 cut(s)	412, 741, 791, 1776, 2112, 2593, 3778
TspGWI	ACGGA	2 cut(s)	3029, 3522
Van91I	CCANNNNNTGG	1 cut(s)	2542
Vha464I	CTTAAG	1 cut(s)	434
VneI	GTGCAC	1 cut(s)	4266
VpaK11BI	GGWCC	3 cut(s)	1809, 2338, 2722
VspI	ATTAAT	1 cut(s)	1574
XagI	CCTNNNNNAGG	2 cut(s)	72, 2076
XapI	RAATTY	5 cut(s)	568, 1257, 1913, 2421, 3824
XbaI	TCTAGA	1 cut(s)	4249
XceI	RCATGY	3 cut(s)	193, 2821, 2825
XhoI	CTCGAG	2 cut(s)	127, 2322
XmiI	GTMKAC	1 cut(s)	2036
ZrmI	AGTACT	3 cut(s)	395, 908, 2934
Zsp2I	ATGCAT	3 cut(s)	1254, 2823, 4498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.