Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0017342

Physical Location & Seq

Genomic Coordinates Reverse (-)

62465372 .. 62470848

Gene Structure 5477 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020211

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4578 bp

ATGGCTGCAGACTCCTCATTCGAGCTGGAAAACTCTATTTCCGGCCACCTCCCACGTTACACCACCCCTATGGCAGGCTCGTCCCGCTCATTCACACCGCTTAGCTCCGCTCACTCGAGAACTCCCAGAAGGTCTCGCCACCCCACTCCAACTACGCCTTTCGCCTCGGACAATGACATGTCGTGGCAAGGCGGGATCTCCTGGCAGTTTGAGCCGACGGGGTGGAACGAAACTAGCAACTTGGGCTCAGCTATGAGCCCATGGGCCACCGCGAGCACGTCCTCAAACCAGGCCCAGCGTGCCCACCGCTCAGCAAATTACTACTACCTCTCCCGCACCACCGGCGGGTTTCGTACCTCACCAAATCAATATTACAACGAGTACTCCAGCTATGGTGCTGTGACAGATGGGAGACTTGAGCTTAAGAGCTACGTTGCCAGGGACAATGAAAGCTCAACTTTTGGGAGGAGTAGTTACAATTATGGGGAGCATAGCAAACCCTTTAAAGCAGTTACTCCAAGGTTGGAGGGTATTAAAGAAGCTGGGAGTGGAAATTTCAGTGGCAGTGGCCCTTTGGCTAAGAAAGATTTGCTTGGTTTGATTGATTATCAAACAGCAGAAGATGATGATGAAGATGAGAGTTTGGGTCATAGTGGACACCATGGTCATGGCCTATATCTTGATCATGGTCATAGGCCATCACACAAAGGACATGATCATGGGTTGTCACCCATGGGTCATCATGGCCATAGTGGGCTGCACCATGGCCATGGGGCAGGATCACATGGTCATGACACGTGGCCGCAATCAGTTACTAGTCACTATATTGATGATGAGAGTGATCAGGGTTCTGGGTATGATGATGATGATGAGGATGAGGATGAGGATGAGGATGATGCAAGGCCAGTGAAGCAAGTTGGGTTGTTTAGCTTGTTCAAGTATTCAACAAAATGGGATATGTTTCTTGTGTTTTTGGGTTGTGTTGGTGCTCTTATCAATGGAGGGTCTCTTCCTTGGTATTCTTATCTCTTTGGAAAGTTTGTGAATAAGATTGCTAAAGAATCAAAGGTTGACAAAGACAACATGATGAGAGATGTAGAGAAGACATGCCTGCTTATGACTGGTTTAGCTGCAATAGTAGTAGTTGGAGCATATATGGAGATCACTTGTTGGAGAATGGTGGGGGAGAGATCTGCTCAGAGAATAAGAAGAGAATATCTAAAAGCAGTTCTGCGACAAGACATTAGCTTTTTTGATACTGAGATTGCTGCTGGTGATATTATGCATGGAATTTCAAGTGATGTTGCTGCAATCCAAGAAGTCATGGGGGAGAAGATGGCACATTTCATTCACCATATATGTACCTTCATATGTGGATACACAGTTGGGTTTCTAAGGTCATGGAAAGTAACTCTAGTAGTCTTCTCAGTCATACCATTGATGATGTTAACTGGTATCGCTTACAAGGCTATTTATGTTGGCCTAACCACAAAAGAGGAGTTGTCTTACAGGACAGCTGGTACTGTAGCCGAGCAAGCTATCAGTTCAATCAGAACAGTATTTTCCTTTGTTGCGGAGGATCATTTGGCTGAAAAATATTCTGCATTAATTGCCAATTTGGTGCCTTTTGGAGCAAAGATTGGCTTTGCTAAGGGTGCAGGAGTCGGAGTTATCTATCTTGTTAGTTATTCTACATGGGCGTTGGCTTTCTGGTACGGTTCTATTTTGGTTGCTAAGGGTGAGATCACTGGAGGTGCTGCCATTGCTTGTTTCTTTGGTGTAAATGTCGGGGGAAGGGGCTTGGCATTGTCACTGTCATACTTTGCTCAGTTTGCACAAGGGACCGTAGCAGCAGGCCGAGTATTTGAAATTATAGATAGAGTTCCAGAAATAGATCCCTACAGCCCAGTAGGTAGGAAACTGTCAAATGTTCGAGGAAGGATTGAATTTAAAGGCATTTATTTCGCATACCCATCTCGTCCTGAAGCTCCAATTTTTCATTCGCTTAATCTGGTCATTCCATCCTCAAAGACACTAGCACTTGTTGGCTCTAGCGGAGGTGGAAAGTCTACGATCTTTGCTCTTATAGAGAGGTTCTATGACCCTACCAAAGGTACAATAACATTGGATGGTCATGATTTAAGGTCACTTCAGGTGAAGTGGCTAAGGGGTCAGATGGGCATGGTTGGTCAGGAGCCAGTGCTCTTTGCCACCAGCATTTTAGAAAACGTGTTGATGGGGAAAGAGAATGCCACCAAGAAAGAAGCAATTACTGCCTGCATTGCTGCCAATGCTCACAGCTTCATTTCTGGACTTCCACATGGCTATGACACTCAGGTTGGAGACAGAGGAACACTACTCTCAGGTGGTCAGAAACAAAGAATTGCATTGGCTCGAGCGATGATCAAGGACCCTAGAATCCTTCTCTTGGATGAGCCTACCAGTGCCTTAGACCCTGAATCCGAGACTGTAGTCCAACAGGCTATTGACAAAATTTCCTCAGGACGAACATCTATTGTAATTGCTCACAGGCTATCAACTGTAAGAAACTCTCATGCCATTGTTGTTCTTGACAGTGGCTCTGTCATTGAGATTGGTAACCACCGCCAGCTCATGGAAAAAGGGGGGGCGTATTATAGCCTCGTTGAGCTTGCTTCTGATGGAGTGACAAAACCTTTTTCGGAACAATATGATACTGGAATTAGCCCTCAGTTTTCCAGGTTTGACAAATCAGCTTGTGATGTATCAAGATCAACATTCATGCAAGATCGAAACCAAGTAGAAGAGAAACAGGTCCAAAAACCAATTCCAAGAAAGGTTCAGCTTTCAGATATATGGTTGTTACAGAGGCCTGAGCTCCCAATGCTATTAGTTGGATTTCTTTTGGGCATGCATGCAGGTGCGATTCTATCTATCTTCCCTTATATTCTAGGCCAAGCACTTGTAGTTTATTTTGATAAAGAGCTATCAAAGATCAAAGCAGGAATCGCGCCCCTTTGCTTAGTACTTGTTGCACTTGGCTTTGGGAATATACTGTTCATGACAGGACAACAAGGCTTGTGTGGCTGGGCTGGAACAAAGCTCACAATGAGAGTGAGAAACCTTCTTTTCCGTTCCATACTTAAACAAGAACCTGGTTGGTTTGATTTTGAAGAAAACTCAAAAGCGGTACTTGTCTCTAGGATTTCAATGGATTCTGTGGCTTTTCGTGCCGTCCATGTTGATCGATTATCAGTCTTGTTTATGGGGTTGAGTTCAGCTATGGTTGGATTGGGTATATCCTTTTGGCTTGAATGGAGGCTAGCTCTTTTGGCTGCTGCTCTCACTCCTTTCACTCTTGGTGCAAGTTACTTGAGCTTGATCATAAATATAGGGCCCAAACTTGACAATGATGCTTATGCCAAGGCTAGCAATATTGCTTCTGGTGCAGTTTCAAACGTAAGGACGGTTGCAACATTTTCTGCTCAAGACCAGTTGATCAAGTCCTTTGATAGTGCTTTATCGGAGCCAAAGACAAAATCAATGAGAAGAACACAAATAATGGGCCTAGCACTCGGGTTCTCTCAAGGTGTTATGTACGGAGCTTACACCTTAACTCTCTTGTTTGGTGCTTACCTTATGAAAGAAAACAAAACTAATTTCGGTGATGTTTACAAAATCTTTCTCATTCTTGTTCTGAGCTCATTTTCTGTGGGACAATTAGCCGGTCTTGCACCAGACACATCTATGGCTGAAACAGCAATTCCAGCAGTTTTTGATATCATGACTCGTAAGCCATTAATCAGCAGTGGCAGAGGAAAAGATAAGAAGATTGAAAGGTCTAAACCTTTAGATATTCACTTCAAAATGGTGACATTTGCATACCCATCAAGGCCTGAGGTAACTGTGTTGAGTAAATTTTCCCTCAAGGTCAAAGGTGGTAGCACAGTGGCATTGGTGGGTGGAAGTGGATCGGGAAAATCAACTGTCATATGGTTAATACAAAGGTTTTATGATCCAACTCAAGGGAAGGTGATGATGGGGGGAGTGGATTTGAGGGAGATTGATGTGAAGTGGTTAAGAAGGCAAACAGCTTTAGTTGGTCAAGAGCCTACGTTGTTTGCTGGCACTATAAGAGAGAACATTGCTTTTGGTAACCCGGATGTTTCATGGGCTGAAATTGAAGGTGCTGCAAGAGAAGCTTACATCCACAGCTTCATTAGTGGCCTCCCTCAAGGCTATCAAACTCAGGTTGGTGAGAGTGGGGTTCAGCTATCTGGGGGGCAGAAGCAAAGGATTGCCATAGCAAGAGCTATACTGAAGAAATCAAAGGTACTACTTCTAGATGAAGCAAGTAGTGCACTAGATTTGGAATCTGAGAGACATATACAAGACGCACTTAGGAAAGTCAGCAAAAGGGCCACAACAATCATAGTCGCTCACCGGCTTTCTACTATCAGGGAAGCTGATATGATAGCAGTTATGAAAGATGGTGGCATCACTGAATATGGGAGCCATGATACTCTCATGAAATCTCATCTTAATGGCGTGTATGCCAGCTTGGTGCGTGCAGAAGCCGAAGCCAATGCATTTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009966 GO:0009987 GO:0010033 GO:0010218 GO:0010315 GO:0010329 GO:0010540 GO:0010541 GO:0010646 GO:0010817 GO:0010928 GO:0015562 GO:0016020 GO:0016043 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0032501 GO:0032502 GO:0032535 GO:0042221 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044464 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048527 GO:0048528 GO:0048583 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0048859 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080161 GO:0090066 GO:0090567 GO:0090691 GO:0090696 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1526

Amino Acids

166.56

Weight (kDa)

8.06

Isoelectric Point (pI)

34.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	323 - 595	8.1e-56	ABC transporter transmembrane region
ABC_tran	PF00005	667 - 815	1.3e-31	ABC transporter
ABC_membrane	PF00664	956 - 1227	4.9e-42	ABC transporter transmembrane region
SMC_N	PF02463	1152 - 1488	1.4e-06	RecF/RecN/SMC N terminal domain
ABC_tran	PF00005	1298 - 1445	3.4e-30	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017342)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g35260
malus_domestica	MD09G1177100.v1.1 MD17G1149200.v1.1
prunus_persica	Prupe.3G036400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0146621
rosa_laevigata	RLG00000020211
rosa_multiflora	Rmu_sc0001986.1_g000032
rosa_roxburghii	Rroxscaffold_2G00100000
rosa_rugosa	Rorug02G0395800 Rorug02G0395800
rosa_samantha	Rh2BG464800 Rh2DG473800
rosa_wichuraiana	Rw2G036930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2888
AasI	GACNNNNNNGTC	1 cut(s)	663
Acc36I	ACCTGC	1 cut(s)	2888
AccB1I	GGYRCC	1 cut(s)	1621
AccB7I	CCANNNNNTGG	1 cut(s)	2614
AccBSI	CCGCTC	3 cut(s)	87, 110, 309
AccI	GTMKAC	1 cut(s)	2069
AccII	CGCG	2 cut(s)	272, 2990
AclWI	GGATC	6 cut(s)	203, 787, 1587, 1889, 3958, 3989
AcoI	YGGCCR	4 cut(s)	43, 745, 766, 800
AcsI	RAATTY	5 cut(s)	553, 1290, 1946, 2493, 3896
AcuI	CTGAAG	3 cut(s)	2004, 2135, 4319
AcvI	CACGTG	1 cut(s)	798
AfiI	CCNNNNNNNGG	6 cut(s)	74, 345, 2111, 2428, 2614, 4004
AflII	CTTAAG	1 cut(s)	422
AflIII	ACRYGT	3 cut(s)	177, 795, 2229
AhlI	ACTAGT	1 cut(s)	815
AjiI	CACGTC	1 cut(s)	279
AjnI	CCWGG	5 cut(s)	200, 288, 437, 2717, 3133
AjuI	GAANNNNNNNTTGG	4 cut(s)	1984, 2016, 2790, 2822
Alw21I	GWGCWC	6 cut(s)	278, 991, 2205, 2859, 3683, 4342
Alw26I	GTCTC	7 cut(s)	138, 406, 1011, 2337, 2459, 3181, 4354
Alw44I	GTGCAC	1 cut(s)	4338
AlwI	GGATC	6 cut(s)	203, 787, 1587, 1889, 3958, 3989
Ama87I	CYCGRG	3 cut(s)	115, 2394, 3554
ApaI	GGGCCC	1 cut(s)	3378
ApaLI	GTGCAC	1 cut(s)	4338
ApoI	RAATTY	5 cut(s)	553, 1290, 1946, 2493, 3896
ArsI	GACNNNNNNTTYG	1 cut(s)	34
AseI	ATTAAT	2 cut(s)	1607, 3779
AspLEI	GCGC	1 cut(s)	2992
AsuC2I	CCSGG	1 cut(s)	4139
AsuNHI	GCTAGC	2 cut(s)	3301, 3407
AvaI	CYCGRG	3 cut(s)	115, 2394, 3554
AvaII	GGWCC	3 cut(s)	1842, 2410, 2794
AxyI	CCTNAGG	2 cut(s)	2500, 3876
BaeGI	GKGCMC	3 cut(s)	304, 3378, 4342
BalI	TGGCCA	2 cut(s)	747, 768
BanI	GGYRCC	1 cut(s)	1621
BanII	GRGCYC	5 cut(s)	248, 260, 2859, 3378, 3683
BbrPI	CACGTG	1 cut(s)	798
BbsI	GAAGAC	2 cut(s)	1109, 1414
Bbv12I	GWGCWC	6 cut(s)	278, 991, 2205, 2859, 3683, 4342
BceAI	ACGGC	1 cut(s)	3197
BcgI	CGANNNNNNTGC	3 cut(s)	1945, 1979, 4547
BciT130I	CCWGG	5 cut(s)	202, 290, 439, 2719, 3135
BciVI	GTATCC	1 cut(s)	1370
BclI	TGATCA	6 cut(s)	682, 715, 841, 2403, 3360, 3477
BcnI	CCSGG	1 cut(s)	4139
BcoDI	GTCTC	7 cut(s)	138, 406, 1011, 2337, 2459, 3181, 4354
BcuI	ACTAGT	1 cut(s)	815
BfmI	CTRYAG	4 cut(s)	6, 1524, 1900, 2469
BfrI	CTTAAG	1 cut(s)	422
BfuAI	ACCTGC	1 cut(s)	2888
BfuI	GTATCC	1 cut(s)	1370
BglII	AGATCT	1 cut(s)	1190
BlpI	GCTNAGC	3 cut(s)	101, 247, 310
BmcAI	AGTACT	2 cut(s)	383, 3006
Bme1390I	CCNGG	6 cut(s)	202, 290, 439, 2719, 3135, 4139
Bme18I	GGWCC	3 cut(s)	1842, 2410, 2794
BmeT110I	CYCGRG	3 cut(s)	115, 2394, 3554
BmgBI	CACGTC	1 cut(s)	279
BmiI	GGNNCC	7 cut(s)	1623, 1843, 2196, 2412, 3376, 3507, 4493
BmrFI	CCNGG	6 cut(s)	202, 290, 439, 2719, 3135, 4139
BmrI	ACTGGG	1 cut(s)	1901
BmsI	GCATC	3 cut(s)	886, 3382, 4485
BmtI	GCTAGC	2 cut(s)	3305, 3411
BmuI	ACTGGG	1 cut(s)	1901
BoxI	GACNNNNGTC	1 cut(s)	2471
BpiI	GAAGAC	2 cut(s)	1109, 1414
BplI	GAGNNNNNCTC	4 cut(s)	1180, 1212, 3289, 3321
BpmI	CTGGAG	2 cut(s)	370, 1770
Bpu10I	CCTNAGC	4 cut(s)	1650, 1734, 2165, 2853
Bpu1102I	GCTNAGC	3 cut(s)	101, 247, 310
BpuEI	CTTGAG	7 cut(s)	437, 3373, 3450, 3549, 3890, 3987, 4197
BpuMI	CCSGG	1 cut(s)	4139
Bsa29I	ATCGAT	1 cut(s)	3226
BsaAI	YACGTR	1 cut(s)	798
BsaI	GGTCTC	2 cut(s)	138, 1011
BsaXI	ACNNNNNCTCC	8 cut(s)	314, 344, 368, 398, 457, 487, 1653, 1683
Bsc4I	CCNNNNNNNGG	6 cut(s)	74, 345, 2111, 2428, 2614, 4004
Bse118I	RCCGGY	3 cut(s)	341, 3704, 4422
Bse1I	ACTGG	9 cut(s)	905, 1126, 1456, 1753, 1907, 2198, 2442, 2701, 3472
Bse21I	CCTNAGG	2 cut(s)	2500, 3876
Bse3DI	GCAATG	3 cut(s)	1761, 2280, 4122
BseBI	CCWGG	5 cut(s)	202, 290, 439, 2719, 3135
BseCI	ATCGAT	1 cut(s)	3226
BseGI	GGATG	9 cut(s)	880, 886, 892, 898, 2021, 2134, 2437, 4147, 4185
BseLI	CCNNNNNNNGG	6 cut(s)	74, 345, 2111, 2428, 2614, 4004
BseMI	GCAATG	3 cut(s)	1761, 2280, 4122
BseNI	ACTGG	9 cut(s)	905, 1126, 1456, 1753, 1907, 2198, 2442, 2701, 3472
BseRI	GAGGAG	3 cut(s)	4, 481, 1511
BseSI	GKGCMC	3 cut(s)	304, 3378, 4342
BseYI	CCCAGC	3 cut(s)	294, 542, 3066
BsgI	GTGCAG	4 cut(s)	743, 1677, 3447, 4569
Bsh1236I	CGCG	2 cut(s)	272, 2990
BshNI	GGYRCC	1 cut(s)	1621
BshVI	ATCGAT	1 cut(s)	3226
BsiHKAI	GWGCWC	6 cut(s)	278, 991, 2205, 2859, 3683, 4342
BsiHKCI	CYCGRG	3 cut(s)	115, 2394, 3554
BsiSI	CCGG	5 cut(s)	42, 342, 3705, 4139, 4423
BslFI	GGGAC	4 cut(s)	67, 455, 1855, 3708
BslI	CCNNNNNNNGG	6 cut(s)	74, 345, 2111, 2428, 2614, 4004
BsmAI	GTCTC	7 cut(s)	138, 406, 1011, 2337, 2459, 3181, 4354
BsmFI	GGGAC	4 cut(s)	67, 455, 1855, 3708
BsmI	GAATGC	1 cut(s)	2254
Bso31I	GGTCTC	2 cut(s)	138, 1011
BsoBI	CYCGRG	3 cut(s)	115, 2394, 3554
Bsp120I	GGGCCC	1 cut(s)	3374
Bsp1720I	GCTNAGC	3 cut(s)	101, 247, 310
Bsp19I	CCATGG	5 cut(s)	260, 661, 732, 763, 769
BspDI	ATCGAT	1 cut(s)	3226
BspFNI	CGCG	2 cut(s)	272, 2990
BspHI	TCATGA	5 cut(s)	790, 2134, 3039, 3762, 4506
BspLI	GGNNCC	7 cut(s)	1623, 1843, 2196, 2412, 3376, 3507, 4493
BspMAI	CTGCAG	1 cut(s)	10
BspMI	ACCTGC	1 cut(s)	2888
BspOI	GCTAGC	2 cut(s)	3305, 3411
BspPI	GGATC	6 cut(s)	203, 787, 1587, 1889, 3958, 3989
BspT107I	GGYRCC	1 cut(s)	1621
BspTI	CTTAAG	1 cut(s)	422
BspTNI	GGTCTC	2 cut(s)	138, 1011
BsrBI	CCGCTC	3 cut(s)	87, 110, 309
BsrDI	GCAATG	3 cut(s)	1761, 2280, 4122
BsrFI	RCCGGY	3 cut(s)	341, 3704, 4422
BsrI	ACTGG	9 cut(s)	905, 1126, 1456, 1753, 1907, 2198, 2442, 2701, 3472
BssAI	RCCGGY	3 cut(s)	341, 3704, 4422
BssT1I	CCWWGG	8 cut(s)	260, 518, 661, 732, 763, 769, 1013, 3402
Bst2UI	CCWGG	5 cut(s)	202, 290, 439, 2719, 3135
Bst6I	CTCTTC	3 cut(s)	1014, 1204, 2778
BstAFI	CTTAAG	1 cut(s)	422
BstAPI	GCANNNNNTGC	3 cut(s)	1610, 2273, 4337
BstBAI	YACGTR	1 cut(s)	798
BstDSI	CCRYGG	5 cut(s)	260, 661, 732, 763, 769
BstEII	GGTNACC	2 cut(s)	2597, 4133
BstENI	CCTNNNNNAGG	2 cut(s)	72, 2109
BstF5I	GGATG	9 cut(s)	880, 886, 892, 898, 2021, 2134, 2437, 4147, 4185
BstFNI	CGCG	2 cut(s)	272, 2990
BstHHI	GCGC	1 cut(s)	2992
BstMAI	GTCTC	7 cut(s)	138, 406, 1011, 2337, 2459, 3181, 4354
BstNI	CCWGG	5 cut(s)	202, 290, 439, 2719, 3135
BstNSI	RCATGY	4 cut(s)	181, 1110, 2893, 2897
BstPAI	GACNNNNGTC	1 cut(s)	2471
BstPI	GGTNACC	2 cut(s)	2597, 4133
BstSCI	CCNGG	6 cut(s)	200, 288, 437, 2717, 3133, 4137
BstSFI	CTRYAG	4 cut(s)	6, 1524, 1900, 2469
BstSLI	GKGCMC	3 cut(s)	304, 3378, 4342
BstUI	CGCG	2 cut(s)	272, 2990
BstV2I	GAAGAC	2 cut(s)	1109, 1414
BstX2I	RGATCY	3 cut(s)	195, 1190, 1894
BstXI	CCANNNNNNTGG	3 cut(s)	70, 668, 770
BstYI	RGATCY	3 cut(s)	195, 1190, 1894
Bsu15I	ATCGAT	1 cut(s)	3226
Bsu36I	CCTNAGG	2 cut(s)	2500, 3876
BsuI	GTATCC	1 cut(s)	1370
BsuTUI	ATCGAT	1 cut(s)	3226
BtgI	CCRYGG	5 cut(s)	260, 661, 732, 763, 769
BtgZI	GCGATG	1 cut(s)	2414
BtrI	CACGTC	1 cut(s)	279
BtsCI	GGATG	9 cut(s)	880, 886, 892, 898, 2021, 2134, 2437, 4147, 4185
BtsI	GCAGTG	2 cut(s)	571, 3793
BveI	ACCTGC	1 cut(s)	2888
CciI	TCATGA	5 cut(s)	790, 2134, 3039, 3762, 4506
CfoI	GCGC	1 cut(s)	2992
Cfr10I	RCCGGY	3 cut(s)	341, 3704, 4422
ClaI	ATCGAT	1 cut(s)	3226
CseI	GACGC	1 cut(s)	4382
CsiI	ACCWGGT	1 cut(s)	3133
DraI	TTTAAA	2 cut(s)	505, 1951
DrdI	GACNNNNNNGTC	1 cut(s)	663
DseDI	GACNNNNNNGTC	1 cut(s)	663
EaeI	YGGCCR	4 cut(s)	43, 745, 766, 800
Eam1104I	CTCTTC	3 cut(s)	1014, 1204, 2778
EarI	CTCTTC	3 cut(s)	1014, 1204, 2778
Ecl136II	GAGCTC	2 cut(s)	2857, 3681
Eco130I	CCWWGG	8 cut(s)	260, 518, 661, 732, 763, 769, 1013, 3402
Eco147I	AGGCCT	2 cut(s)	2851, 3874
Eco24I	GRGCYC	5 cut(s)	248, 260, 2859, 3378, 3683
Eco31I	GGTCTC	2 cut(s)	138, 1011
Eco32I	GATATC	1 cut(s)	3760
Eco47I	GGWCC	3 cut(s)	1842, 2410, 2794
Eco53kI	GAGCTC	2 cut(s)	2857, 3681
Eco57I	CTGAAG	3 cut(s)	2004, 2135, 4319
Eco72I	CACGTG	1 cut(s)	798
Eco81I	CCTNAGG	2 cut(s)	2500, 3876
Eco88I	CYCGRG	3 cut(s)	115, 2394, 3554
Eco91I	GGTNACC	2 cut(s)	2597, 4133
EcoICRI	GAGCTC	2 cut(s)	2857, 3681
EcoNI	CCTNNNNNAGG	2 cut(s)	72, 2109
EcoO109I	RGGNCCY	2 cut(s)	2410, 3374
EcoO65I	GGTNACC	2 cut(s)	2597, 4133
EcoRII	CCWGG	5 cut(s)	200, 288, 437, 2717, 3133
EcoRV	GATATC	1 cut(s)	3760
EcoT14I	CCWWGG	8 cut(s)	260, 518, 661, 732, 763, 769, 1013, 3402
EcoT22I	ATGCAT	3 cut(s)	1287, 2895, 4570
EcoT38I	GRGCYC	5 cut(s)	248, 260, 2859, 3378, 3683
ErhI	CCWWGG	8 cut(s)	260, 518, 661, 732, 763, 769, 1013, 3402
FaqI	GGGAC	4 cut(s)	67, 455, 1855, 3708
FauI	CCCGC	4 cut(s)	92, 185, 338, 341
FauNDI	CATATG	2 cut(s)	1370, 3971
FbaI	TGATCA	6 cut(s)	682, 715, 841, 2403, 3360, 3477
FblI	GTMKAC	1 cut(s)	2069
FokI	GGATG	9 cut(s)	887, 893, 899, 905, 2008, 2141, 2444, 4154, 4172
FriOI	GRGCYC	5 cut(s)	248, 260, 2859, 3378, 3683
GlaI	GCGC	1 cut(s)	2991
GsaI	CCCAGC	3 cut(s)	298, 546, 3070
GsuI	CTGGAG	2 cut(s)	370, 1770
HapII	CCGG	5 cut(s)	42, 342, 3705, 4139, 4423
HgaI	GACGC	1 cut(s)	4382
HhaI	GCGC	1 cut(s)	2992
Hin6I	GCGC	1 cut(s)	2990
HinP1I	GCGC	1 cut(s)	2990
HincII	GTYRAC	2 cut(s)	1072, 1449
HindII	GTYRAC	2 cut(s)	1072, 1449
HindIII	AAGCTT	1 cut(s)	4179
HpaI	GTTAAC	1 cut(s)	1449
HpaII	CCGG	5 cut(s)	42, 342, 3705, 4139, 4423
Hpy166II	GTNNAC	6 cut(s)	656, 1072, 1449, 2070, 3652, 4340
Hpy8I	GTNNAC	6 cut(s)	656, 1072, 1449, 2070, 3652, 4340
Hpy99I	CGWCG	1 cut(s)	220
HpyAV	CCTTC	9 cut(s)	123, 1375, 1788, 1932, 2432, 3113, 4003, 4056, 4157
HpyCH4IV	ACGT	7 cut(s)	55, 278, 432, 797, 2229, 3438, 4094
HpySE526I	ACGT	7 cut(s)	55, 278, 432, 797, 2229, 3438, 4094
HspAI	GCGC	1 cut(s)	2990
Ksp22I	TGATCA	6 cut(s)	682, 715, 841, 2403, 3360, 3477
KspAI	GTTAAC	1 cut(s)	1449
LweI	GCATC	3 cut(s)	886, 3382, 4485
MabI	ACCWGGT	1 cut(s)	3133
MaeII	ACGT	7 cut(s)	55, 278, 432, 797, 2229, 3438, 4094
MbiI	CCGCTC	3 cut(s)	87, 110, 309
MflI	RGATCY	3 cut(s)	195, 1190, 1894
MlsI	TGGCCA	2 cut(s)	747, 768
MluNI	TGGCCA	2 cut(s)	747, 768
MlyI	GAGTC	3 cut(s)	5, 1671, 3760
Mox20I	TGGCCA	2 cut(s)	747, 768
Mph1103I	ATGCAT	3 cut(s)	1287, 2895, 4570
MscI	TGGCCA	2 cut(s)	747, 768
MslI	CAYNNNNRTG	9 cut(s)	68, 666, 717, 768, 789, 2325, 2898, 3725, 4521
Msp20I	TGGCCA	2 cut(s)	747, 768
MspA1I	CMGCKG	1 cut(s)	1517
MspCI	CTTAAG	1 cut(s)	422
MspI	CCGG	5 cut(s)	42, 342, 3705, 4139, 4423
MspR9I	CCNGG	6 cut(s)	202, 290, 439, 2719, 3135, 4139
Mva1269I	GAATGC	1 cut(s)	2254
MvaI	CCWGG	5 cut(s)	202, 290, 439, 2719, 3135
MvnI	CGCG	2 cut(s)	272, 2990
NciI	CCSGG	1 cut(s)	4139
NcoI	CCATGG	5 cut(s)	260, 661, 732, 763, 769
NdeI	CATATG	2 cut(s)	1370, 3971
NheI	GCTAGC	2 cut(s)	3301, 3407
NlaIV	GGNNCC	7 cut(s)	1623, 1843, 2196, 2412, 3376, 3507, 4493
NmeAIII	GCCGAG	2 cut(s)	1555, 1883
NmuCI	GTSAC	7 cut(s)	400, 726, 818, 1809, 2145, 2665, 3850
NsiI	ATGCAT	3 cut(s)	1287, 2895, 4570
NspI	RCATGY	4 cut(s)	181, 1110, 2893, 2897
PaeI	GCATGC	2 cut(s)	2893, 2897
PaeR7I	CTCGAG	2 cut(s)	115, 2394
PagI	TCATGA	5 cut(s)	790, 2134, 3039, 3762, 4506
PaqCI	CACCTGC	1 cut(s)	2888
PceI	AGGCCT	2 cut(s)	2851, 3874
PciI	ACATGT	1 cut(s)	177
PctI	GAATGC	1 cut(s)	2254
PfeI	GAWTC	7 cut(s)	1061, 2418, 2459, 2905, 2985, 3194, 4352
PflMI	CCANNNNNTGG	1 cut(s)	2614
PleI	GAGTC	3 cut(s)	5, 1670, 3760
PmaCI	CACGTG	1 cut(s)	798
PmlI	CACGTG	1 cut(s)	798
PpsI	GAGTC	3 cut(s)	5, 1670, 3760
Ppu21I	YACGTR	1 cut(s)	798
PpuMI	RGGWCCY	1 cut(s)	2410
PscI	ACATGT	1 cut(s)	177
PshAI	GACNNNNGTC	1 cut(s)	2471
PshBI	ATTAAT	2 cut(s)	1607, 3779
Psp124BI	GAGCTC	2 cut(s)	2859, 3683
Psp5II	RGGWCCY	1 cut(s)	2410
Psp6I	CCWGG	5 cut(s)	200, 288, 437, 2717, 3133
PspCI	CACGTG	1 cut(s)	798
PspEI	GGTNACC	2 cut(s)	2597, 4133
PspFI	CCCAGC	3 cut(s)	294, 542, 3066
PspGI	CCWGG	5 cut(s)	200, 288, 437, 2717, 3133
PspN4I	GGNNCC	7 cut(s)	1623, 1843, 2196, 2412, 3376, 3507, 4493
PspOMI	GGGCCC	1 cut(s)	3374
PspPPI	RGGWCCY	1 cut(s)	2410
PspXI	VCTCGAGB	1 cut(s)	2394
PstI	CTGCAG	1 cut(s)	10
PsuI	RGATCY	3 cut(s)	195, 1190, 1894
PvuII	CAGCTG	1 cut(s)	1517
RseI	CAYNNNNRTG	9 cut(s)	68, 666, 717, 768, 789, 2325, 2898, 3725, 4521
SacI	GAGCTC	2 cut(s)	2859, 3683
ScaI	AGTACT	2 cut(s)	383, 3006
SchI	GAGTC	3 cut(s)	5, 1671, 3760
ScrFI	CCNGG	6 cut(s)	202, 290, 439, 2719, 3135, 4139
SexAI	ACCWGGT	1 cut(s)	3133
SfaNI	GCATC	3 cut(s)	886, 3382, 4485
SfcI	CTRYAG	4 cut(s)	6, 1524, 1900, 2469
Sfr274I	CTCGAG	2 cut(s)	115, 2394
SgrAI	CRCCGGYG	1 cut(s)	341
SinI	GGWCC	3 cut(s)	1842, 2410, 2794
SlaI	CTCGAG	2 cut(s)	115, 2394
SmiMI	CAYNNNNRTG	9 cut(s)	68, 666, 717, 768, 789, 2325, 2898, 3725, 4521
SpeI	ACTAGT	1 cut(s)	815
SphI	GCATGC	2 cut(s)	2893, 2897
SseBI	AGGCCT	2 cut(s)	2851, 3874
SspI	AATATT	3 cut(s)	371, 1598, 3415
SstI	GAGCTC	2 cut(s)	2859, 3683
StuI	AGGCCT	2 cut(s)	2851, 3874
StyD4I	CCNGG	6 cut(s)	200, 288, 437, 2717, 3133, 4137
StyI	CCWWGG	8 cut(s)	260, 518, 661, 732, 763, 769, 1013, 3402
TaiI	ACGT	7 cut(s)	58, 281, 435, 800, 2232, 3441, 4097
TaqI	TCGA	6 cut(s)	21, 116, 1933, 2395, 2770, 3226
TatI	WGTACW	2 cut(s)	381, 3004
TauI	GCSGC	1 cut(s)	805
TfiI	GAWTC	7 cut(s)	1061, 2418, 2459, 2905, 2985, 3194, 4352
TseFI	GTSAC	7 cut(s)	400, 726, 818, 1809, 2145, 2665, 3850
Tsp45I	GTSAC	7 cut(s)	400, 726, 818, 1809, 2145, 2665, 3850
TspGWI	ACGGA	2 cut(s)	3101, 3594
Van91I	CCANNNNNTGG	1 cut(s)	2614
Vha464I	CTTAAG	1 cut(s)	422
VneI	GTGCAC	1 cut(s)	4338
VpaK11BI	GGWCC	3 cut(s)	1842, 2410, 2794
VspI	ATTAAT	2 cut(s)	1607, 3779
XagI	CCTNNNNNAGG	2 cut(s)	72, 2109
XapI	RAATTY	5 cut(s)	553, 1290, 1946, 2493, 3896
XbaI	TCTAGA	1 cut(s)	4321
XceI	RCATGY	4 cut(s)	181, 1110, 2893, 2897
XhoI	CTCGAG	2 cut(s)	115, 2394
XmiI	GTMKAC	1 cut(s)	2069
ZrmI	AGTACT	2 cut(s)	383, 3006
Zsp2I	ATGCAT	3 cut(s)	1287, 2895, 4570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.