RchiOBHm_Chr2g0148181

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009960
Physical Location & Seq
Forward (+)
65821474 .. 65823026
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGATGTCCACCCAAGTGTTCATACTTTGTGACAAGCCTAAAGATGCAAATTTCGTATTGGTAAGTTTCCGGGGGACAGAACCTTTTGATGCTGATGATTGGAGTACTGATTTTGACTACTCTTGGTATGAAATTCCAGAATTGGGTAAAGTACACATGGGATTCTTGGAAGCCTTAGGCTTGGGCAACAGAACTGATGTTGTAAACTTCTACAATCAAATTTTTGTATACAATCAACTTCAAGAGAAACACAAGCTCACCCCTCAAAAAGGTGTTGATGGTAGCAAAAGGCCTTCAGAAGGTATTAAAGCACTATCCTCTAATGGCATCAAGATAGTTCCACCAGATATGGTAGAGAAGAGTGCATACTATGCTGTAAGAAAAAAGCTCAAGAGCTTACTAGAGGAGCACAAAAACGCAAAATTCGTTGTTACAGGGCATAGTCTAGGTGGGGCACTTGCGATATTGTTCCCTTGTGTGCTGGTGTTGCATGAGGAGATGGAGTTAATGGATAGGTTGTTGGGTATATATACATTTGGACAGCCAAGGATAGGGAATAGGCAGCTGGGGAGGTACATGGAAGTCCATCTGAATAAGCCGGTCCAAAAGTACTTCAGGGTTGTCTACTCCAACGATCTTGTCCCCAGGTTGCCTTATGATGACAGAACCTTCTTGTTTAAACATTTCGGTGTCTGCCTTTACTACGACAGCCTATACAATGAACATGTAACTAAGGAGGAGCCAAACAGAAATTTCCTTGGACTGAGATTTCTAATACCATTGTATCTGACTGCCGCTTGGGAGCTACTAAGAAGTTTTACAATGGGGTACGTATATGGACCAGAGTACAAAGAAGGGTGGTTTTCCATTCTGCTAAGGATGGTAGGACTTGTTCTTCCTGGTTTCGCTGCACATTCCCCTACAAATTATGTTAACTCGGTGAGGCTTGGAAAGGAACGGCTCGTCCAAAGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

325

Amino Acids

37.49

Weight (kDa)

8.52

Isoelectric Point (pI)

32.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 21 - 220 5.7e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 228, 624
AciI CCGC 1 cut(s) 795
AcsI RAATTY 5 cut(s) 49, 132, 219, 422, 751
AcuI CTGAAG 2 cut(s) 279, 598
AfaI GTAC 6 cut(s) 106, 153, 575, 611, 830, 848
AfiI CCNNNNNNNGG 4 cut(s) 143, 269, 299, 551
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 1 cut(s) 242
AjnI CCWGG 2 cut(s) 644, 898
AleI CACNNNNGTG 1 cut(s) 14
AloI GAACNNNNNNTCC 1 cut(s) 948
AluBI AGCT 5 cut(s) 256, 388, 396, 565, 805
AluI AGCT 5 cut(s) 256, 388, 396, 565, 805
Alw21I GWGCWC 1 cut(s) 411
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 2 cut(s) 562, 908
ApoI RAATTY 5 cut(s) 49, 132, 219, 422, 751
AspS9I GGNCC 2 cut(s) 601, 839
AsuC2I CCSGG 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 250, 952
AvaII GGWCC 2 cut(s) 601, 839
AxyI CCTNAGG 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 457
BaeI ACNNNNGTAYC 2 cut(s) 767, 800
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 2 cut(s) 574, 895
BccI CCATC 4 cut(s) 272, 493, 594, 874
BceAI ACGGC 1 cut(s) 974
BciT130I CCWGG 2 cut(s) 646, 900
BcnI CCSGG 1 cut(s) 71
BfaI CTAG 2 cut(s) 401, 446
BisI GCNGC 3 cut(s) 563, 795, 909
BlsI GCNGC 3 cut(s) 564, 796, 910
BmcAI AGTACT 2 cut(s) 106, 611
Bme1390I CCNGG 3 cut(s) 71, 646, 900
Bme18I GGWCC 2 cut(s) 601, 839
BmgT120I GGNCC 2 cut(s) 601, 839
BmiI GGNNCC 1 cut(s) 741
BmrFI CCNGG 3 cut(s) 71, 646, 900
BmsI GCATC 3 cut(s) 34, 79, 336
Bpu10I CCTNAGC 1 cut(s) 875
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 71
BsaAI YACGTR 1 cut(s) 832
BsaJI CCNNGG 4 cut(s) 70, 545, 644, 757
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 269, 299, 551
Bse118I RCCGGY 1 cut(s) 598
Bse21I CCTNAGG 1 cut(s) 175
BseBI CCWGG 2 cut(s) 646, 900
BseDI CCNNGG 4 cut(s) 70, 545, 644, 757
BseGI GGATG 1 cut(s) 885
BseLI CCNNNNNNNGG 4 cut(s) 143, 269, 299, 551
BseMII CTCAG 1 cut(s) 755
BseRI GAGGAG 3 cut(s) 419, 509, 752
BseSI GKGCMC 1 cut(s) 457
BseXI GCAGC 2 cut(s) 574, 895
BseYI CCCAGC 1 cut(s) 565
BsgI GTGCAG 1 cut(s) 894
BshFI GGCC 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 411
BsiSI CCGG 2 cut(s) 70, 599
BslFI GGGAC 2 cut(s) 88, 626
BslI CCNNNNNNNGG 4 cut(s) 143, 269, 299, 551
BsmFI GGGAC 2 cut(s) 88, 626
BsnI GGCC 1 cut(s) 292
Bsp1286I GDGCHC 2 cut(s) 411, 457
Bsp143I GATC 1 cut(s) 634
BspACI CCGC 1 cut(s) 795
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 741
BsrFI RCCGGY 1 cut(s) 598
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 4 cut(s) 70, 545, 644, 757
BssMI GATC 1 cut(s) 634
BssNAI GTATAC 1 cut(s) 229
BssT1I CCWWGG 2 cut(s) 545, 757
Bst1107I GTATAC 1 cut(s) 229
Bst2UI CCWGG 2 cut(s) 646, 900
Bst6I CTCTTC 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 371
BstBAI YACGTR 1 cut(s) 832
BstDEI CTNAG 5 cut(s) 175, 732, 764, 809, 875
BstENI CCTNNNNNAGG 2 cut(s) 267, 297
BstF5I GGATG 1 cut(s) 885
BstKTI GATC 1 cut(s) 637
BstMBI GATC 1 cut(s) 634
BstMWI GCNNNNNNNGC 2 cut(s) 371, 487
BstNI CCWGG 2 cut(s) 646, 900
BstNSI RCATGY 1 cut(s) 728
BstSCI CCNGG 3 cut(s) 69, 644, 898
BstSLI GKGCMC 1 cut(s) 457
BstSNI TACGTA 1 cut(s) 832
BstV1I GCAGC 2 cut(s) 574, 895
BstZ17I GTATAC 1 cut(s) 229
Bsu36I CCTNAGG 1 cut(s) 175
BsuRI GGCC 1 cut(s) 292
BtsCI GGATG 1 cut(s) 885
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 2 cut(s) 601, 839
Csp6I GTAC 6 cut(s) 105, 152, 574, 610, 829, 847
CviAII CATG 4 cut(s) 157, 491, 577, 725
CviQI GTAC 6 cut(s) 105, 152, 574, 610, 829, 847
DdeI CTNAG 5 cut(s) 175, 732, 764, 809, 875
DpnI GATC 1 cut(s) 636
DpnII GATC 1 cut(s) 634
DraI TTTAAA 1 cut(s) 679
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco105I TACGTA 1 cut(s) 832
Eco130I CCWWGG 2 cut(s) 545, 757
Eco147I AGGCCT 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 601, 839
Eco57I CTGAAG 2 cut(s) 279, 598
Eco81I CCTNAGG 1 cut(s) 175
EcoNI CCTNNNNNAGG 2 cut(s) 267, 297
EcoRII CCWGG 2 cut(s) 644, 898
EcoT14I CCWWGG 2 cut(s) 545, 757
ErhI CCWWGG 2 cut(s) 545, 757
FaeI CATG 4 cut(s) 160, 494, 580, 728
FaqI GGGAC 2 cut(s) 88, 626
FatI CATG 4 cut(s) 156, 490, 576, 724
FblI GTMKAC 2 cut(s) 228, 624
Fnu4HI GCNGC 3 cut(s) 563, 795, 909
FokI GGATG 1 cut(s) 892
Fsp4HI GCNGC 3 cut(s) 563, 795, 909
FspBI CTAG 2 cut(s) 401, 446
GluI GCNGC 3 cut(s) 563, 795, 909
GsaI CCCAGC 1 cut(s) 569
HaeIII GGCC 1 cut(s) 292
HapII CCGG 2 cut(s) 70, 599
Hin1II CATG 4 cut(s) 160, 494, 580, 728
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HinfI GANTC 1 cut(s) 162
HpaI GTTAAC 1 cut(s) 934
HpaII CCGG 2 cut(s) 70, 599
HphI GGTGA 2 cut(s) 250, 952
Hpy166II GTNNAC 6 cut(s) 9, 154, 205, 229, 625, 934
Hpy188I TCNGA 3 cut(s) 298, 591, 789
Hpy188III TCNNGA 4 cut(s) 137, 242, 331, 391
Hpy8I GTNNAC 6 cut(s) 9, 154, 205, 229, 625, 934
HpyAV CCTTC 4 cut(s) 293, 303, 679, 848
HpyCH4IV ACGT 1 cut(s) 831
HpyCH4V TGCA 4 cut(s) 47, 365, 490, 911
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 487
HpyF3I CTNAG 5 cut(s) 175, 732, 764, 809, 875
HpySE526I ACGT 1 cut(s) 831
Hsp92II CATG 4 cut(s) 160, 494, 580, 728
KspAI GTTAAC 1 cut(s) 934
Kzo9I GATC 1 cut(s) 634
LmnI GCTCC 3 cut(s) 406, 739, 802
Lsp1109I GCAGC 2 cut(s) 574, 895
LweI GCATC 3 cut(s) 34, 79, 336
MaeI CTAG 2 cut(s) 401, 446
MaeII ACGT 1 cut(s) 831
MaeIII GTNAC 3 cut(s) 29, 430, 727
MalI GATC 1 cut(s) 636
MboI GATC 1 cut(s) 634
MboII GAAGA 2 cut(s) 370, 887
MhlI GDGCHC 2 cut(s) 411, 457
MluCI AATT 7 cut(s) 49, 132, 140, 219, 422, 751, 925
MmeI TCCRAC 1 cut(s) 654
MnlI CCTC 7 cut(s) 273, 328, 397, 487, 564, 730, 936
MseI TTAA 5 cut(s) 306, 506, 678, 933, 976
MslI CAYNNNNRTG 2 cut(s) 14, 687
MspA1I CMGCKG 1 cut(s) 565
MspI CCGG 2 cut(s) 70, 599
MspR9I CCNGG 3 cut(s) 71, 646, 900
MssI GTTTAAAC 1 cut(s) 679
MvaI CCWGG 2 cut(s) 646, 900
MwoI GCNNNNNNNGC 2 cut(s) 371, 487
NciI CCSGG 1 cut(s) 71
NdeII GATC 1 cut(s) 634
NlaIII CATG 4 cut(s) 160, 494, 580, 728
NlaIV GGNNCC 1 cut(s) 741
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 728
OliI CACNNNNGTG 1 cut(s) 14
PceI AGGCCT 1 cut(s) 292
PciI ACATGT 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 3 cut(s) 564, 796, 910
PmeI GTTTAAAC 1 cut(s) 679
Ppu21I YACGTR 1 cut(s) 832
PscI ACATGT 1 cut(s) 724
Psp6I CCWGG 2 cut(s) 644, 898
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 2 cut(s) 644, 898
PspN4I GGNNCC 1 cut(s) 741
PspPI GGNCC 2 cut(s) 601, 839
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PvuII CAGCTG 1 cut(s) 565
RsaI GTAC 6 cut(s) 106, 153, 575, 611, 830, 848
RsaNI GTAC 6 cut(s) 105, 152, 574, 610, 829, 847
RseI CAYNNNNRTG 2 cut(s) 14, 687
SaqAI TTAA 5 cut(s) 306, 506, 678, 933, 976
SatI GCNGC 3 cut(s) 563, 795, 909
Sau3AI GATC 1 cut(s) 634
Sau96I GGNCC 2 cut(s) 601, 839
ScaI AGTACT 2 cut(s) 106, 611
ScrFI CCNGG 3 cut(s) 71, 646, 900
SduI GDGCHC 2 cut(s) 411, 457
SfaNI GCATC 3 cut(s) 34, 79, 336
SinI GGWCC 2 cut(s) 601, 839
SmiMI CAYNNNNRTG 2 cut(s) 14, 687
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
SnaBI TACGTA 1 cut(s) 832
Sse9I AATT 7 cut(s) 49, 132, 140, 219, 422, 751, 925
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 1 cut(s) 795
SspMI CTAG 2 cut(s) 401, 446
StuI AGGCCT 1 cut(s) 292
StyD4I CCNGG 3 cut(s) 69, 644, 898
StyI CCWWGG 2 cut(s) 545, 757
TaiI ACGT 1 cut(s) 834
TasI AATT 7 cut(s) 49, 132, 140, 219, 422, 751, 925
TatI WGTACW 4 cut(s) 104, 151, 609, 846
TauI GCSGC 1 cut(s) 797
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 5 cut(s) 306, 506, 678, 933, 976
Tru9I TTAA 5 cut(s) 306, 506, 678, 933, 976
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 2 cut(s) 562, 908
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 10, 144, 735
VpaK11BI GGWCC 2 cut(s) 601, 839
XagI CCTNNNNNAGG 2 cut(s) 267, 297
XapI RAATTY 5 cut(s) 49, 132, 219, 422, 751
XceI RCATGY 1 cut(s) 728
XmiI GTMKAC 2 cut(s) 228, 624
XspI CTAG 2 cut(s) 401, 446
ZrmI AGTACT 2 cut(s) 106, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.