RchiOBHm_Chr2g0148191

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017699
Physical Location & Seq
Forward (+)
65826658 .. 65827318
661 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGTCTGTGAAGCAAGGGCTGCTTGGTGTCTACACATTTGCGCAACAAATGATAGGAGATTGCCAGCTGGGGAGGTACGTTGAGGCACATTTGGTTAATCCGGTCCCAAAGTACTTCAGGGTTGTCTACTGCAATGATTTTCTGCCCAGGTTGCCTTATGATGAACGAAACTGCTTCTATAAGCATTTTGGGGTGTGTCTTTACTATGACAGTCTATATATTGAACAAAAAATGGAGGAGAAGGAGGGGCCAAACAGAAATTACTTTGGAATGAGACATCTGATGGCAGAAATTCTGAATGCTGCTTGGGAGCTAATGAGAAGTTTGACAATGGGTTACATATATGGGCCAGATTATGCAGAAGGGTGGTTCTCCATTTTGGCAAGGATAGCAGGAGTACTTGTGTTTCCTCGCATGACGGCACATTCACTTACGGATTACATCAACTCGGTGAGACTTGGGAAGGAGCGCATCATTCAAGCGTCCTCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

164

Amino Acids

19.08

Weight (kDa)

7.66

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 8 - 54 6.9e-06 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017699)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g36220
malus_domestica MD17G1155700.v1.1
rosa_chinensis RchiOBHm_Chr2g0148191
rosa_laevigata RLG00000020325
rosa_multiflora Rmu_co8278977.1_g000001 Rmu_sc0001516.1_g000104
rosa_roxburghii Rroxscaffold_2G00099010
rosa_rugosa Rorug02G0404300.1
rosa_samantha Rh2BG476100 Rh2DG484800 Rh6BG483600 Rh6DG474800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 42
AccI GTMKAC 2 cut(s) 30, 126
AcsI RAATTY 1 cut(s) 291
AcuI CTGAAG 1 cut(s) 100
AfaI GTAC 3 cut(s) 77, 113, 399
AgsI TTSAA 2 cut(s) 224, 479
AjnI CCWGG 1 cut(s) 146
AluBI AGCT 2 cut(s) 67, 313
AluI AGCT 2 cut(s) 67, 313
Alw26I GTCTC 2 cut(s) 268, 448
AoxI GGCC 2 cut(s) 248, 347
ApeKI GCWGC 2 cut(s) 19, 302
ApoI RAATTY 1 cut(s) 291
AspLEI GCGC 2 cut(s) 43, 471
AspS9I GGNCC 3 cut(s) 103, 248, 347
AsuHPI GGTGA 1 cut(s) 463
AvaII GGWCC 1 cut(s) 103
BbvI GCAGC 2 cut(s) 6, 289
BccI CCATC 1 cut(s) 277
BceAI ACGGC 1 cut(s) 435
BciT130I CCWGG 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 268, 448
BisI GCNGC 2 cut(s) 20, 303
BlsI GCNGC 2 cut(s) 21, 304
BmcAI AGTACT 2 cut(s) 113, 399
Bme1390I CCNGG 1 cut(s) 148
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 103, 248, 347
BmiI GGNNCC 2 cut(s) 105, 249
BmrFI CCNGG 1 cut(s) 148
BmsI GCATC 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 387, 417
Bse3DI GCAATG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 139
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 2 cut(s) 6, 289
BseYI CCCAGC 1 cut(s) 67
BshFI GGCC 2 cut(s) 250, 349
BsiSI CCGG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 89
BsmAI GTCTC 2 cut(s) 268, 448
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 2 cut(s) 250, 349
BspANI GGCC 2 cut(s) 250, 349
BspLI GGNNCC 2 cut(s) 105, 249
BsrDI GCAATG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 1 cut(s) 212
BstAPI GCANNNNNTGC 1 cut(s) 19
BstC8I GCNNGC 1 cut(s) 65
BstHHI GCGC 2 cut(s) 43, 471
BstMAI GTCTC 2 cut(s) 268, 448
BstMWI GCNNNNNNNGC 3 cut(s) 19, 151, 389
BstNI CCWGG 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 6, 289
BsuRI GGCC 2 cut(s) 250, 349
Cac8I GCNNGC 1 cut(s) 65
CfoI GCGC 2 cut(s) 43, 471
Cfr13I GGNCC 3 cut(s) 103, 248, 347
CseI GACGC 1 cut(s) 471
Csp6I GTAC 3 cut(s) 76, 112, 398
CviAII CATG 1 cut(s) 415
CviJI RGCY 5 cut(s) 19, 67, 250, 313, 349
CviKI_1 RGCY 5 cut(s) 19, 67, 250, 313, 349
CviQI GTAC 3 cut(s) 76, 112, 398
Eco47I GGWCC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 146
FaeI CATG 1 cut(s) 418
FalI AAGNNNNNCTT 1 cut(s) 38
FaqI GGGAC 1 cut(s) 89
FatI CATG 1 cut(s) 414
FblI GTMKAC 2 cut(s) 30, 126
Fnu4HI GCNGC 2 cut(s) 20, 303
Fsp4HI GCNGC 2 cut(s) 20, 303
FspI TGCGCA 1 cut(s) 42
GlaI GCGC 2 cut(s) 42, 470
GluI GCNGC 2 cut(s) 20, 303
GsaI CCCAGC 1 cut(s) 71
HaeIII GGCC 2 cut(s) 250, 349
HapII CCGG 1 cut(s) 101
HgaI GACGC 1 cut(s) 471
HhaI GCGC 2 cut(s) 43, 471
Hin1II CATG 1 cut(s) 418
Hin6I GCGC 2 cut(s) 41, 469
HinP1I GCGC 2 cut(s) 41, 469
HpaII CCGG 1 cut(s) 101
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 2 cut(s) 31, 127
Hpy188I TCNGA 2 cut(s) 282, 297
Hpy8I GTNNAC 2 cut(s) 31, 127
HpyAV CCTTC 3 cut(s) 235, 356, 457
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 2 cut(s) 132, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 151, 389
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 1 cut(s) 418
HspAI GCGC 2 cut(s) 41, 469
LmnI GCTCC 2 cut(s) 310, 466
LpnPI CCDG 8 cut(s) 53, 77, 103, 114, 133, 160, 363, 378
Lsp1109I GCAGC 2 cut(s) 6, 289
LweI GCATC 1 cut(s) 480
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 335
MboII GAAGA 1 cut(s) 480
MluCI AATT 2 cut(s) 259, 291
MnlI CCTC 5 cut(s) 66, 76, 229, 238, 420
MseI TTAA 1 cut(s) 96
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 1 cut(s) 148
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 1 cut(s) 148
MwoI GCNNNNNNNGC 3 cut(s) 19, 151, 389
NlaIII CATG 1 cut(s) 418
NlaIV GGNNCC 2 cut(s) 105, 249
NsbI TGCGCA 1 cut(s) 42
PctI GAATGC 1 cut(s) 304
PkrI GCNGC 2 cut(s) 21, 304
Psp6I CCWGG 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 2 cut(s) 105, 249
PspPI GGNCC 3 cut(s) 103, 248, 347
PvuII CAGCTG 1 cut(s) 67
RsaI GTAC 3 cut(s) 77, 113, 399
RsaNI GTAC 3 cut(s) 76, 112, 398
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 2 cut(s) 20, 303
Sau96I GGNCC 3 cut(s) 103, 248, 347
ScaI AGTACT 2 cut(s) 113, 399
ScrFI CCNGG 1 cut(s) 148
SetI ASST 5 cut(s) 69, 77, 81, 152, 315
SfaNI GCATC 1 cut(s) 480
SinI GGWCC 1 cut(s) 103
Sse9I AATT 2 cut(s) 259, 291
StyD4I CCNGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 212
TaiI ACGT 1 cut(s) 81
TasI AATT 2 cut(s) 259, 291
TatI WGTACW 2 cut(s) 111, 397
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseI GCWGC 2 cut(s) 19, 302
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 103
XapI RAATTY 1 cut(s) 291
XmiI GTMKAC 2 cut(s) 30, 126
ZrmI AGTACT 2 cut(s) 113, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.