RchiOBHm_Chr2g0152461

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015155
Physical Location & Seq
Reverse (-)
69832635 .. 69839800
7166 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3705 bp
ATGGTGAGGACGAAGAAAGGAGGACGACCGGCCACTCTGATTTCAGAAGAGGCAAGGATCCGAATACGTGACATACTCCAGCAGTTCCTGGAGTCCAATGAAGAAGTTTACACATTTGGGACAGACCTGACGGTTAATGAACGTGCTTACGTCCACGAACAGTGCAGCAAGATGGATATGAAATCCAAGAGCTCCGGGTCGGGTAAAACGCGCAAGGTTTCGGTTTACAGGCTTTTTAGGATGGAGGCAATCAGCAATGGTAAGGAAAGAATCCCTCAGGTGACGTTTTCCGGGGACACAAGAGAAGTTTTGGGGGATTTGTTTACGTGGTACCCACCCGGAGATGGAGAGGAACCCAAAGAAAGGATCCGTGAAGATAGTGACAAGTTTCAGAAACTCCACCGCAAGAAAGACCCGCTGTTCTGCAGACCTTCGATGAGCCAGAACGAAATTGCAAAGAAAGTGGAAATGCAGGCTGCCAAAATGAGAAAAGCCAAGATTACTAAAGAGAGGTCTAACCTACCCATGGCACCCTACAGGGATGTGGTAACGTCAGCAGTTGAATCTCACCAGGTTGTTCTTGTATCTGGCGAGACTGGGTGCGGAAAGACCACACAGGTGCCACAATATCTTTTGGACCATATGTGGGGTAAGGGTGAGGCATGTAAAATAGTGTGCACTCAACCCAGGCGTATCTCAGCCATATCAGTTGCTGAGAGAATCTCTTATGAAAGAGGACAGAATGTTGGGGAGGATATTGGGTACAAGATACGGTTGGAAAGCAAAGGTGGGAGGCACTCATCTGTGGTGTTCTGCACAAATGGGATTCTACTGAGGATTCTAATTTCCAGAGGCGGTGGTAGGTCAAAAACAGATGCATCGAATAAAGAAACCAAACAACAAGATCTCTCTGACATAACTCACATTATTGTGGATGAAATTCATGAAAGGGATCGCCACTCTGATTTCATTCTGGCTATTCTCAGAGACATGCTTCCTTCGCATCCTCATCTGCATCTGATATTGATGAGTGCGACTGTTGATGTGGAACGGTTTTCACAATATTTTGGTGGCTGCCCAATTATCCGTGTCCCAGGGTTCACTTATCCTGTCAAATCTTTCTATTTGGAGGATGTATTGTCTATCTTGAGATCTGTAGAAAATAATCACTTAGATAACCCAGCATTAGGTGCCTCAACTGCAGACCGTGACTTATCAGAGGAAGATAAGATTGCTTTAGATGAAGCAATTAGTTTGGCTTGGTCAGATGATGAATTTGATCCACTTCTAGAATTGGTTTCTACTGAAGGAACCCGTAAAATATTTAATTACCAGCATTCTGTAACTGGACTTACACCATTAATGGTATTTGCTGGAAGGGGTAGAGTAGGAGATGTTTGCATGCTGCTATCATTTGGTGCAGACTGCCAATTACGGGCCAAGGATGGAACCACTGCATTAGAGTTGGCTGAGCAGGTAAACCAATTAGAATCTGCTCAGATGTTGAAAGAGCATATGGAAAGTGCTCGTTCTAACTCTATGGAAGAACAAAATTTGCTCAATAAATATCTCCAATATTTTAATCCTGAGAATATTGATTTCATTCTTATTGAGCAATTGTTAGGGAAAATTTTTAGTGATTCGAAAGATGGAGCTATTCTTGTTTTTCTTCCAGGGTGGGATGATATAAATAAAATGAGGGACAGATTACTCACGAACCCATATTTCAGAGATCCTTCTAAGTGTGTGATTATATCTCTACATTCAATGGTTCCAGCTGCTGAACAGAAGAAGGTCTTCATTCGTCCACCTAGTGGTTGCCGAAAAATTGTTCTTTCAACCAATATTGCTGAAACTGCAGTCACTATTGATGATGTTGTTTATGTTATAAACACGGGTCGGATGAAAGAAAAAAATTATGATCCTTTTAGTAATGTTTCAACTCTTCAATCTTCTTGGGTGTCAAAAGCGAATGCCAAGCAGCGACAGGGGCGTGCGGGCCGTTGTCAGCCTGGAATCTGTTATCATCTTTACTCTAAACTTCGAGCAGCTGCATTGTCAGATTTTCAGGTGCCTGAGATTAGGAGAATGCCAATCGAGGAACTTTGCCTTCAGGTGAAGTTGCTTGATCCGGATTGCAAGATAGACGAGTTCCTAAACAAGACGCTGGATCCTCCTCATTCTGAGACCATACACAATGCGATTTCTGTTCTTCGAGATATCGGGGCTTTGTCGGTGGACGATAAACTTACAGAACTTGGGGAAAAGCTAGGTTCTTTACCTGTTCATCCATTGACAAGCAAGATGCTATTCTTTGCCACACTGATGAACTGTCTCGACCCAGCTTTAACTCTGGCCTGTGCTGCCGACTACAAGGATCCATTCTCTCTTCCTATGATGCCTGAGGAAAAGGAGAAAGCTGCTGCTGCTAAGGCTGAACTGTGTTCATTGTATGGTGGGAACAGTGATCAGCTAGCAGTCATAGCAGCATTTGACTGCTGGAAGATTGCAAAAGAACAGGGTAAAGAGAATCGGTTCTGTGCTAAATACTTTGTCTCTGCAAGTACCATGCAGATGCTATCTGGTATGCGAAAGCAACTCCAAACAGAGCTAATTCGGCTGGGGTTTATTCCTGAGGATGTGTCTAGATGTAGCTTGAATGCACGCAATCCAGGTATAATCCACGCTGTCCTTGTGGCTGGTCTGTATCCCATGTTGGGAAGATTGCTTTCTTCTACTAAGAGTGGGAAAAAAAAAGGATCCTTTGTAGAAATTCCTGATGGTAGTAAAGTTCGTTTGCACCCTCGATCTGCTAATTTTAAAATAGCATTCAAGGCACGTGGTGGCTCTCCTTTGGTTGTATATGATGAGATAACCCGTGGGGATGGGGGTTCGAATATACGAAACTGTACTGTTGTCGGGCCACTTCCATTACTATTGCTGTCGACAGAAATAGCTGTTGCTCCAGCCAATGATGATGAGGGAAGTGATGTCGATGATAATGAGTATGAAAGTGATGAAGATGCAATGAAGATAGATTCTAAGTCGGGTGAACAGGCCGAAGAGAAGATTATGTCATCACCTGATAAATCTGTCATGGTTATAGTGGATGGGTGGCTTTGTTTTGAGTTGAAAGCTCTTGACGTTGCTCAGATCTATTGCTTGAGGGAACGCCTGTCTGCAGCAATTCTATTTACGGTTACTCATCCAAAGGAAGTTTTACCTCCCGATCTTGGGGCATCTATTTATGCTATAGCCTGTGTTCTATCTTATGATGGTCTATCTGGGATTCCATTGCCAAAGCCTATGGATTTGCCGAATTCAGATTTAGAACTTGAGATCGGTAAAGATGGAAAGGCACAGTTTAAAGTGACAGGAAATAAAGCAGCAAAATCAAAGGAGCCATTACGTCAGAGAGTAGTTTGTCAGAGGTCTCTATCGCGAGCTACTGATTTACCTGCCTTAAATGGATTTGCAAAGTTGTGTGACGACCCATCAATTCAGAGCGTTCCCCCACCGGCTTTCGCGAACGCTACTGATTTACCTGCCTTAATAAATGGAGTTGCAAAGTTGAGTGGCGACCGATCAATTCACAGTGCTCCCCCACCAGCTTTTGCGAACACAATCCCGCCGCCCACTTCACAAGTTCCAGCAGGTCCTGTCGCAGGAAACAAGCGGAAGAAGCGCCGGAATCAGGCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007292 GO:0007346 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0009994 GO:0010033 GO:0010467 GO:0012505 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019899 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0032392 GO:0032501 GO:0032502 GO:0032504 GO:0032508 GO:0032991 GO:0034097 GO:0034458 GO:0034612 GO:0034641 GO:0035770 GO:0035821 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043900 GO:0043902 GO:0043903 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044788 GO:0044794 GO:0044827 GO:0044829 GO:0045069 GO:0045070 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0048232 GO:0048468 GO:0048477 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048599 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050792 GO:0050794 GO:0050896 GO:0051276 GO:0051445 GO:0051446 GO:0051702 GO:0051704 GO:0051726 GO:0051728 GO:0051729 GO:0051817 GO:0051851 GO:0060184 GO:0065007 GO:0070035 GO:0070063 GO:0070555 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:0140098 GO:1901360 GO:1901363 GO:1903900 GO:1903902 GO:1990247 GO:1990904 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind R3H

Protein Analysis

1234

Amino Acids

136.56

Weight (kDa)

7.07

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H PF01424 20 - 77 2.3e-08 R3H domain
DEAD PF00270 186 - 348 4.3e-11 DEAD/DEAH box helicase
Helicase_C PF00271 540 - 669 7.4e-11 Helicase conserved C-terminal domain
HA2_C PF21010 764 - 825 1.6e-08 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 894 - 984 7.4e-11 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30800 AT2G30800
fragaria_vesca FvH4_6g38870
malus_domestica MD09G1146500.v1.1
prunus_persica Prupe.3G179000_v2.0.a1
pyrus_communis pycom09g06680
rosa_chinensis RchiOBHm_Chr2g0152461
rosa_laevigata RLG00000020635
rosa_multiflora Rmu_sc0005366.1_g000012
rosa_roxburghii Rroxscaffold_2G00095700
rosa_rugosa Rorug02G0432900
rosa_samantha Rh2AG494700 Rh2BG506900 Rh2CG480900 Rh2DG518100
rosa_wichuraiana Rw2G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1889
Acc36I ACCTGC 4 cut(s) 1465, 3466, 3553, 3644
Acc65I GGTACC 1 cut(s) 330
AccB1I GGYRCC 5 cut(s) 330, 529, 619, 1190, 2071
AccB7I CCANNNNNTGG 1 cut(s) 1814
AccI GTMKAC 1 cut(s) 2945
AccII CGCG 3 cut(s) 211, 3442, 3527
AccIII TCCGGA 1 cut(s) 2131
AciI CCGC 8 cut(s) 403, 416, 603, 855, 1997, 3629, 3632, 3676
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 6 cut(s) 939, 1274, 1552, 1629, 2772, 3319
AcuI CTGAAG 2 cut(s) 1326, 2096
AcvI CACGTG 1 cut(s) 2840
AdeI CACNNNGTG 2 cut(s) 1814, 2843
AfaI GTAC 4 cut(s) 332, 764, 2566, 2911
AgsI TTSAA 9 cut(s) 563, 1507, 1767, 1839, 1941, 1949, 2659, 2833, 3133
AjnI CCWGG 7 cut(s) 87, 570, 686, 1093, 1672, 2011, 2671
AleI CACNNNNGTG 1 cut(s) 617
Alw21I GWGCWC 4 cut(s) 194, 680, 1528, 3601
Alw26I GTCTC 6 cut(s) 587, 981, 2180, 2339, 2560, 3438
Alw44I GTGCAC 1 cut(s) 676
AlwNI CAGNNNCTG 4 cut(s) 88, 713, 1781, 3659
Aor13HI TCCGGA 1 cut(s) 2131
AoxI GGCC 6 cut(s) 30, 1437, 1999, 2355, 2921, 3057
ApaLI GTGCAC 1 cut(s) 676
ApoI RAATTY 6 cut(s) 939, 1274, 1552, 1629, 2772, 3319
AseI ATTAAT 1 cut(s) 1361
Asp700I GAANNNNTTC 2 cut(s) 1796, 2534
Asp718I GGTACC 1 cut(s) 330
AspLEI GCGC 2 cut(s) 213, 3687
AspS9I GGNCC 5 cut(s) 637, 1437, 1999, 2921, 3656
AsuC2I CCSGG 3 cut(s) 196, 292, 339
AsuHPI GGTGA 7 cut(s) 16, 292, 560, 668, 2128, 3062, 3072
AsuII TTCGAA 2 cut(s) 1643, 2894
AsuNHI GCTAGC 1 cut(s) 2473
AvaII GGWCC 2 cut(s) 637, 3656
AxyI CCTNAGG 3 cut(s) 276, 2403, 2634
BaeGI GKGCMC 1 cut(s) 680
BamHI GGATCC 5 cut(s) 57, 366, 2170, 2377, 2759
BanI GGYRCC 5 cut(s) 330, 529, 619, 1190, 2071
BanII GRGCYC 1 cut(s) 194
BbrPI CACGTG 1 cut(s) 2840
BbsI GAAGAC 1 cut(s) 1789
Bbv12I GWGCWC 4 cut(s) 194, 680, 1528, 3601
BceAI ACGGC 1 cut(s) 1986
BciT130I CCWGG 7 cut(s) 89, 572, 688, 1095, 1674, 2013, 2673
BciVI GTATCC 1 cut(s) 2718
BclI TGATCA 1 cut(s) 2467
BcnI CCSGG 3 cut(s) 196, 292, 339
BcoDI GTCTC 6 cut(s) 587, 981, 2180, 2339, 2560, 3438
BfaI CTAG 5 cut(s) 1289, 1812, 2270, 2474, 2646
BfmI CTRYAG 7 cut(s) 424, 535, 1155, 1200, 1857, 3180, 3252
BfoI RGCGCY 1 cut(s) 3688
BfuAI ACCTGC 4 cut(s) 1465, 3466, 3553, 3644
BfuI GTATCC 1 cut(s) 2718
BglII AGATCT 3 cut(s) 904, 1151, 3153
BlpI GCTNAGC 1 cut(s) 1470
Bme18I GGWCC 2 cut(s) 637, 3656
BmgT120I GGNCC 5 cut(s) 637, 1437, 1999, 2921, 3656
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 9 cut(s) 865, 887, 1012, 1024, 2295, 2388, 2565, 3013, 3248
BmtI GCTAGC 1 cut(s) 2477
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 1789
BplI GAGNNNNNCTC 2 cut(s) 707, 739
BpmI CTGGAG 3 cut(s) 62, 110, 2949
Bpu10I CCTNAGC 1 cut(s) 2430
Bpu1102I GCTNAGC 1 cut(s) 1470
Bpu14I TTCGAA 2 cut(s) 1643, 2894
BpuEI CTTGAG 3 cut(s) 1168, 3184, 3356
BpuMI CCSGG 3 cut(s) 196, 292, 339
BsaAI YACGTR 3 cut(s) 68, 327, 2840
BsaI GGTCTC 2 cut(s) 2180, 3438
BsaJI CCNNGG 8 cut(s) 291, 525, 686, 1093, 1094, 1440, 1673, 2878
BsaWI WCCGGW 1 cut(s) 2131
Bse118I RCCGGY 2 cut(s) 28, 3517
Bse1I ACTGG 2 cut(s) 601, 1351
Bse21I CCTNAGG 3 cut(s) 276, 2403, 2634
Bse3DI GCAATG 3 cut(s) 262, 3033, 3293
BseAI TCCGGA 1 cut(s) 2131
BseBI CCWGG 7 cut(s) 89, 572, 688, 1095, 1674, 2013, 2673
BseDI CCNNGG 8 cut(s) 291, 525, 686, 1093, 1094, 1440, 1673, 2878
BseMI GCAATG 3 cut(s) 262, 3033, 3293
BseNI ACTGG 2 cut(s) 601, 1351
BseRI GAGGAG 1 cut(s) 2166
BseSI GKGCMC 1 cut(s) 680
BseYI CCCAGC 3 cut(s) 1180, 2341, 2620
BsgI GTGCAG 3 cut(s) 184, 799, 1440
Bsh1236I CGCG 3 cut(s) 211, 3442, 3527
Bsh1285I CGRYCG 2 cut(s) 29, 3583
BshFI GGCC 6 cut(s) 32, 1439, 2001, 2357, 2923, 3059
BshNI GGYRCC 5 cut(s) 330, 529, 619, 1190, 2071
BsiEI CGRYCG 2 cut(s) 29, 3583
BsiHKAI GWGCWC 4 cut(s) 194, 680, 1528, 3601
BsiSI CCGG 7 cut(s) 29, 195, 291, 339, 2132, 3518, 3688
BslFI GGGAC 4 cut(s) 133, 308, 1076, 1715
BsmAI GTCTC 6 cut(s) 587, 981, 2180, 2339, 2560, 3438
BsmFI GGGAC 4 cut(s) 133, 308, 1076, 1715
BsmI GAATGC 5 cut(s) 1336, 1978, 2094, 2665, 2828
BsnI GGCC 6 cut(s) 32, 1439, 2001, 2357, 2923, 3059
Bso31I GGTCTC 2 cut(s) 2180, 3438
Bsp119I TTCGAA 2 cut(s) 1643, 2894
Bsp1286I GDGCHC 4 cut(s) 194, 680, 1528, 3601
Bsp13I TCCGGA 1 cut(s) 2131
Bsp1720I GCTNAGC 1 cut(s) 1470
Bsp19I CCATGG 1 cut(s) 525
Bsp68I TCGCGA 2 cut(s) 3442, 3527
BspACI CCGC 8 cut(s) 403, 416, 603, 855, 1997, 3629, 3632, 3676
BspANI GGCC 6 cut(s) 32, 1439, 2001, 2357, 2923, 3059
BspEI TCCGGA 1 cut(s) 2131
BspFNI CGCG 3 cut(s) 211, 3442, 3527
BspHI TCATGA 1 cut(s) 943
BspMAI CTGCAG 4 cut(s) 428, 1204, 1861, 3184
BspMI ACCTGC 4 cut(s) 1465, 3466, 3553, 3644
BspOI GCTAGC 1 cut(s) 2477
BspT104I TTCGAA 2 cut(s) 1643, 2894
BspT107I GGYRCC 5 cut(s) 330, 529, 619, 1190, 2071
BspTNI GGTCTC 2 cut(s) 2180, 3438
BsrDI GCAATG 3 cut(s) 262, 3033, 3293
BsrFI RCCGGY 2 cut(s) 28, 3517
BsrI ACTGG 2 cut(s) 601, 1351
BssAI RCCGGY 2 cut(s) 28, 3517
BssECI CCNNGG 8 cut(s) 291, 525, 686, 1093, 1094, 1440, 1673, 2878
BssT1I CCWWGG 2 cut(s) 525, 1440
Bst2UI CCWGG 7 cut(s) 89, 572, 688, 1095, 1674, 2013, 2673
Bst6I CTCTTC 4 cut(s) 42, 1950, 2394, 3057
BstAPI GCANNNNNTGC 1 cut(s) 1190
BstBAI YACGTR 3 cut(s) 68, 327, 2840
BstBI TTCGAA 2 cut(s) 1643, 2894
BstC8I GCNNGC 7 cut(s) 474, 1403, 1995, 1999, 2475, 2665, 3444
BstDSI CCRYGG 2 cut(s) 525, 2878
BstENI CCTNNNNNAGG 1 cut(s) 3663
BstFNI CGCG 3 cut(s) 211, 3442, 3527
BstH2I RGCGCY 1 cut(s) 3688
BstHHI GCGC 2 cut(s) 213, 3687
BstMAI GTCTC 6 cut(s) 587, 981, 2180, 2339, 2560, 3438
BstMCI CGRYCG 2 cut(s) 29, 3583
BstNI CCWGG 7 cut(s) 89, 572, 688, 1095, 1674, 2013, 2673
BstNSI RCATGY 3 cut(s) 666, 994, 1405
BstSFI CTRYAG 7 cut(s) 424, 535, 1155, 1200, 1857, 3180, 3252
BstSLI GKGCMC 1 cut(s) 680
BstUI CGCG 3 cut(s) 211, 3442, 3527
BstV2I GAAGAC 1 cut(s) 1789
BstX2I RGATCY 9 cut(s) 57, 366, 904, 1151, 1732, 2170, 2377, 2759, 3153
BstYI RGATCY 9 cut(s) 57, 366, 904, 1151, 1732, 2170, 2377, 2759, 3153
Bsu36I CCTNAGG 3 cut(s) 276, 2403, 2634
BsuI GTATCC 1 cut(s) 2718
BsuRI GGCC 6 cut(s) 32, 1439, 2001, 2357, 2923, 3059
BtgI CCRYGG 2 cut(s) 525, 2878
BtsI GCAGTG 1 cut(s) 1452
BtsIMutI CAGTG 5 cut(s) 167, 1452, 2321, 2470, 3601
BtuMI TCGCGA 2 cut(s) 3442, 3527
BveI ACCTGC 4 cut(s) 1465, 3466, 3553, 3644
Cac8I GCNNGC 7 cut(s) 474, 1403, 1995, 1999, 2475, 2665, 3444
CaiI CAGNNNCTG 4 cut(s) 88, 713, 1781, 3659
CciI TCATGA 1 cut(s) 943
CfoI GCGC 2 cut(s) 213, 3687
Cfr10I RCCGGY 2 cut(s) 28, 3517
Cfr13I GGNCC 5 cut(s) 637, 1437, 1999, 2921, 3656
CseI GACGC 1 cut(s) 2173
CsiI ACCWGGT 1 cut(s) 570
Csp6I GTAC 4 cut(s) 331, 763, 2565, 2910
CspCI CAANNNNNGTGG 4 cut(s) 444, 479, 2821, 2856
CviAII CATG 8 cut(s) 526, 663, 944, 991, 1402, 2569, 2713, 3097
CviQI GTAC 4 cut(s) 331, 763, 2565, 2910
DraI TTTAAA 2 cut(s) 2821, 3367
DraIII CACNNNGTG 2 cut(s) 1814, 2843
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 4 cut(s) 42, 1950, 2394, 3057
EarI CTCTTC 4 cut(s) 42, 1950, 2394, 3057
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 525, 1440
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 2 cut(s) 2180, 3438
Eco32I GATATC 1 cut(s) 2221
Eco47I GGWCC 2 cut(s) 637, 3656
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 2 cut(s) 1326, 2096
Eco72I CACGTG 1 cut(s) 2840
Eco81I CCTNAGG 3 cut(s) 276, 2403, 2634
EcoICRI GAGCTC 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 3663
EcoO109I RGGNCCY 1 cut(s) 3656
EcoRI GAATTC 1 cut(s) 3319
EcoRII CCWGG 7 cut(s) 87, 570, 686, 1093, 1672, 2011, 2671
EcoRV GATATC 1 cut(s) 2221
EcoT14I CCWWGG 2 cut(s) 525, 1440
EcoT22I ATGCAT 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 525, 1440
FaeI CATG 8 cut(s) 529, 666, 947, 994, 1405, 2572, 2716, 3100
FalI AAGNNNNNCTT 2 cut(s) 1781, 1813
FaqI GGGAC 4 cut(s) 133, 308, 1076, 1715
FatI CATG 8 cut(s) 525, 662, 943, 990, 1401, 2568, 2712, 3096
FauI CCCGC 3 cut(s) 423, 1990, 3636
FauNDI CATATG 2 cut(s) 642, 1515
FbaI TGATCA 1 cut(s) 2467
FblI GTMKAC 1 cut(s) 2945
FriOI GRGCYC 1 cut(s) 194
FspBI CTAG 5 cut(s) 1289, 1812, 2270, 2474, 2646
GlaI GCGC 2 cut(s) 212, 3686
GsaI CCCAGC 3 cut(s) 1184, 2345, 2624
GsuI CTGGAG 3 cut(s) 62, 110, 2949
HaeII RGCGCY 1 cut(s) 3688
HaeIII GGCC 6 cut(s) 32, 1439, 2001, 2357, 2923, 3059
HapII CCGG 7 cut(s) 29, 195, 291, 339, 2132, 3518, 3688
HgaI GACGC 1 cut(s) 2173
HhaI GCGC 2 cut(s) 213, 3687
Hin1II CATG 8 cut(s) 529, 666, 947, 994, 1405, 2572, 2716, 3100
Hin6I GCGC 2 cut(s) 211, 3685
HinP1I GCGC 2 cut(s) 211, 3685
HincII GTYRAC 1 cut(s) 2946
HindII GTYRAC 1 cut(s) 2946
HpaII CCGG 7 cut(s) 29, 195, 291, 339, 2132, 3518, 3688
HphI GGTGA 7 cut(s) 16, 292, 560, 668, 2128, 3062, 3072
HpyAV CCTTC 7 cut(s) 441, 1008, 1301, 1371, 1746, 1786, 2120
HpyCH4IV ACGT 9 cut(s) 67, 142, 150, 284, 326, 551, 2839, 3144, 3409
HpySE526I ACGT 9 cut(s) 67, 142, 150, 284, 326, 551, 2839, 3144, 3409
Hsp92II CATG 8 cut(s) 529, 666, 947, 994, 1405, 2572, 2716, 3100
HspAI GCGC 2 cut(s) 211, 3685
Kpn2I TCCGGA 1 cut(s) 2131
KpnI GGTACC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 2467
LmnI GCTCC 5 cut(s) 197, 1652, 2968, 3400, 3604
LweI GCATC 9 cut(s) 865, 887, 1012, 1024, 2295, 2388, 2565, 3013, 3248
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 5 cut(s) 1289, 1812, 2270, 2474, 2646
MaeII ACGT 9 cut(s) 67, 142, 150, 284, 326, 551, 2839, 3144, 3409
MfeI CAATTG 1 cut(s) 1616
MflI RGATCY 9 cut(s) 57, 366, 904, 1151, 1732, 2170, 2377, 2759, 3153
MhlI GDGCHC 4 cut(s) 194, 680, 1528, 3601
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 2 cut(s) 756, 1880
Mph1103I ATGCAT 1 cut(s) 880
MroI TCCGGA 1 cut(s) 2131
MroXI GAANNNNTTC 2 cut(s) 1796, 2534
MslI CAYNNNNRTG 4 cut(s) 617, 929, 2324, 2573
MspA1I CMGCKG 3 cut(s) 418, 1778, 2051
MspI CCGG 7 cut(s) 29, 195, 291, 339, 2132, 3518, 3688
MunI CAATTG 1 cut(s) 1616
Mva1269I GAATGC 5 cut(s) 1336, 1978, 2094, 2665, 2828
MvaI CCWGG 7 cut(s) 89, 572, 688, 1095, 1674, 2013, 2673
MvnI CGCG 3 cut(s) 211, 3442, 3527
NciI CCSGG 3 cut(s) 196, 292, 339
NcoI CCATGG 1 cut(s) 525
NdeI CATATG 2 cut(s) 642, 1515
NheI GCTAGC 1 cut(s) 2473
NlaIII CATG 8 cut(s) 529, 666, 947, 994, 1405, 2572, 2716, 3100
NmuCI GTSAC 7 cut(s) 68, 280, 380, 1208, 1861, 3370, 3485
NruI TCGCGA 2 cut(s) 3442, 3527
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 3 cut(s) 666, 994, 1405
NspV TTCGAA 2 cut(s) 1643, 2894
OliI CACNNNNGTG 1 cut(s) 617
PaeI GCATGC 1 cut(s) 1405
PagI TCATGA 1 cut(s) 943
PasI CCCWGGG 1 cut(s) 1094
PctI GAATGC 5 cut(s) 1336, 1978, 2094, 2665, 2828
PdmI GAANNNNTTC 2 cut(s) 1796, 2534
PflMI CCANNNNNTGG 1 cut(s) 1814
PfoI TCCNGGA 1 cut(s) 87
PleI GAGTC 1 cut(s) 100
PmaCI CACGTG 1 cut(s) 2840
PmlI CACGTG 1 cut(s) 2840
PpsI GAGTC 1 cut(s) 100
Ppu21I YACGTR 3 cut(s) 68, 327, 2840
PpuMI RGGWCCY 1 cut(s) 3656
PshBI ATTAAT 1 cut(s) 1361
PsiI TTATAA 1 cut(s) 1889
Psp124BI GAGCTC 1 cut(s) 194
Psp5II RGGWCCY 1 cut(s) 3656
Psp6I CCWGG 7 cut(s) 87, 570, 686, 1093, 1672, 2011, 2671
PspCI CACGTG 1 cut(s) 2840
PspFI CCCAGC 3 cut(s) 1180, 2341, 2620
PspGI CCWGG 7 cut(s) 87, 570, 686, 1093, 1672, 2011, 2671
PspPI GGNCC 5 cut(s) 637, 1437, 1999, 2921, 3656
PspPPI RGGWCCY 1 cut(s) 3656
PstI CTGCAG 4 cut(s) 428, 1204, 1861, 3184
PstNI CAGNNNCTG 4 cut(s) 88, 713, 1781, 3659
PsuI RGATCY 9 cut(s) 57, 366, 904, 1151, 1732, 2170, 2377, 2759, 3153
PvuII CAGCTG 2 cut(s) 1778, 2051
RruI TCGCGA 2 cut(s) 3442, 3527
RsaI GTAC 4 cut(s) 332, 764, 2566, 2911
RsaNI GTAC 4 cut(s) 331, 763, 2565, 2910
RseI CAYNNNNRTG 4 cut(s) 617, 929, 2324, 2573
SacI GAGCTC 1 cut(s) 194
SalI GTCGAC 1 cut(s) 2944
Sau96I GGNCC 5 cut(s) 637, 1437, 1999, 2921, 3656
SchI GAGTC 1 cut(s) 101
SduI GDGCHC 4 cut(s) 194, 680, 1528, 3601
SexAI ACCWGGT 1 cut(s) 570
SfaNI GCATC 9 cut(s) 865, 887, 1012, 1024, 2295, 2388, 2565, 3013, 3248
SfcI CTRYAG 7 cut(s) 424, 535, 1155, 1200, 1857, 3180, 3252
SfuI TTCGAA 2 cut(s) 1643, 2894
SinI GGWCC 2 cut(s) 637, 3656
SmiMI CAYNNNNRTG 4 cut(s) 617, 929, 2324, 2573
SmlI CTYRAG 3 cut(s) 1147, 3163, 3335
SmoI CTYRAG 3 cut(s) 1147, 3163, 3335
SphI GCATGC 1 cut(s) 1405
SsiI CCGC 8 cut(s) 403, 416, 603, 855, 1997, 3629, 3632, 3676
SspI AATATT 5 cut(s) 1064, 1323, 1577, 1594, 1846
SspMI CTAG 5 cut(s) 1289, 1812, 2270, 2474, 2646
SstI GAGCTC 1 cut(s) 194
StyI CCWWGG 2 cut(s) 525, 1440
TaiI ACGT 9 cut(s) 70, 145, 153, 287, 329, 554, 2842, 3147, 3412
TaqII GACCGA 1 cut(s) 3597
TatI WGTACW 1 cut(s) 2909
TauI GCSGC 1 cut(s) 3634
TscAI CASTG 5 cut(s) 167, 1459, 2328, 2470, 3601
TseFI GTSAC 7 cut(s) 68, 280, 380, 1208, 1861, 3370, 3485
Tsp45I GTSAC 7 cut(s) 68, 280, 380, 1208, 1861, 3370, 3485
TspGWI ACGGA 2 cut(s) 359, 1076
TspRI CASTG 5 cut(s) 167, 1459, 2328, 2470, 3601
Van91I CCANNNNNTGG 1 cut(s) 1814
VneI GTGCAC 1 cut(s) 676
VpaK11BI GGWCC 2 cut(s) 637, 3656
VspI ATTAAT 1 cut(s) 1361
XagI CCTNNNNNAGG 1 cut(s) 3663
XapI RAATTY 6 cut(s) 939, 1274, 1552, 1629, 2772, 3319
XbaI TCTAGA 2 cut(s) 1288, 2645
XceI RCATGY 3 cut(s) 666, 994, 1405
XmiI GTMKAC 1 cut(s) 2945
XmnI GAANNNNTTC 2 cut(s) 1796, 2534
XspI CTAG 5 cut(s) 1289, 1812, 2270, 2474, 2646
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.