RchiOBHm_Chr1g0328931

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0019336
Physical Location & Seq
Reverse (-)
18394869 .. 18401134
6266 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 450 bp
ATGGATGGGAATACATCTCGGGTAGATGGTGATGATGTTGACTGGGATACTGATGATGAGCTTGAGATTGAAAACTTCACTTTATCTTCTTCAGCTAATCCAGGCTCTGGGGAGGCTCTTGAAAGTAGTCCTCCGGAGCAACATTCTCTGCCAGAGCTGCAGTCTCCTCCAGAACAGTGGTCTCCTCAAGAACAGCAACAAATTGACTCAGATCTCTTTTCTTCAGATTATGATGGGAGCTTTCTAGATGGTTTTTCAGATCTCGATAGCTCTGATGATGAGGTAACCATCGATCCTACATCTGAGGCGGAGAGAAAACTTATGTCCTTGGTTAAAATGGGGTACAATGAATTGGAGGCATCAGTGGCAATAGAAAGATGCGATGTCAGCTCGGAAACTGGGACTTATGGTGACTTCCTGACAGTTGGCCCTGGCATTGAGAACTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

149

Amino Acids

16.22

Weight (kDa)

4.05

Isoelectric Point (pI)

62.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019336)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0321571 RchiOBHm_Chr1g0328931 RchiOBHm_Chr2g0172481
rosa_multiflora Rmu_sc0003413.1_g000021 Rmu_sc0033684.1_g000002
rosa_roxburghii Rroxscaffold_2G00115020
rosa_samantha Rh1AG052900 Rh3AG281200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 107
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 1 cut(s) 308
AclWI GGATC 1 cut(s) 287
AcuI CTGAAG 2 cut(s) 75, 207
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 1 cut(s) 107
AgsI TTSAA 2 cut(s) 71, 122
AjnI CCWGG 2 cut(s) 100, 430
AluBI AGCT 6 cut(s) 61, 95, 157, 240, 270, 390
AluI AGCT 6 cut(s) 61, 95, 157, 240, 270, 390
Alw26I GTCTC 2 cut(s) 168, 186
AlwI GGATC 1 cut(s) 287
AlwNI CAGNNNCTG 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 18
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 428
AsuHPI GGTGA 2 cut(s) 41, 422
AvaI CYCGRG 1 cut(s) 18
BbvI GCAGC 1 cut(s) 144
BccI CCATC 4 cut(s) 20, 227, 242, 296
BciT130I CCWGG 2 cut(s) 102, 432
BciVI GTATCC 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 168, 186
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 1 cut(s) 158
BfuI GTATCC 1 cut(s) 40
BglII AGATCT 2 cut(s) 211, 259
BisI GCNGC 1 cut(s) 158
BlsI GCNGC 1 cut(s) 159
Bme1390I CCNGG 2 cut(s) 102, 432
BmeT110I CYCGRG 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 428
BmrFI CCNGG 2 cut(s) 102, 432
BmrI ACTGGG 2 cut(s) 52, 408
BmsI GCATC 2 cut(s) 368, 368
BmuI ACTGGG 2 cut(s) 52, 408
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 2 cut(s) 83, 171
Bsa29I ATCGAT 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 327, 430
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse1I ACTGG 2 cut(s) 47, 403
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 2 cut(s) 102, 432
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 327, 430
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMII CTCAG 2 cut(s) 222, 294
BseNI ACTGG 2 cut(s) 47, 403
BseRI GAGGAG 2 cut(s) 156, 174
BseXI GCAGC 1 cut(s) 144
BshFI GGCC 1 cut(s) 429
BshVI ATCGAT 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 2 cut(s) 168, 186
BsmFI GGGAC 1 cut(s) 415
BsnI GGCC 1 cut(s) 429
Bso31I GGTCTC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 18
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 3 cut(s) 211, 259, 292
BspACI CCGC 1 cut(s) 308
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 2 cut(s) 221, 295
BspDI ATCGAT 1 cut(s) 291
BspEI TCCGGA 1 cut(s) 133
BspMAI CTGCAG 1 cut(s) 162
BspPI GGATC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 186
BsrI ACTGG 2 cut(s) 47, 403
BssECI CCNNGG 2 cut(s) 327, 430
BssMI GATC 3 cut(s) 211, 259, 292
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 102, 432
Bst4CI ACNGT 2 cut(s) 177, 424
BstDEI CTNAG 2 cut(s) 208, 303
BstEII GGTNACC 1 cut(s) 283
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 3 cut(s) 214, 262, 295
BstMAI GTCTC 2 cut(s) 168, 186
BstMBI GATC 3 cut(s) 211, 259, 292
BstMWI GCNNNNNNNGC 3 cut(s) 157, 365, 387
BstNI CCWGG 2 cut(s) 102, 432
BstPI GGTNACC 1 cut(s) 283
BstSCI CCNGG 2 cut(s) 100, 430
BstSFI CTRYAG 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 144
BstX2I RGATCY 2 cut(s) 211, 259
BstXI CCANNNNNNTGG 1 cut(s) 177
BstYI RGATCY 2 cut(s) 211, 259
Bsu15I ATCGAT 1 cut(s) 291
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 1 cut(s) 429
BsuTUI ATCGAT 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 2 cut(s) 182, 369
CaiI CAGNNNCTG 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 428
ClaI ATCGAT 1 cut(s) 291
Csp6I GTAC 1 cut(s) 343
CviJI RGCY 9 cut(s) 61, 95, 105, 116, 157, 240, 270, 390, 429
CviKI_1 RGCY 9 cut(s) 61, 95, 105, 116, 157, 240, 270, 390, 429
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 2 cut(s) 208, 303
DpnI GATC 3 cut(s) 213, 261, 294
DpnII GATC 3 cut(s) 211, 259, 292
EciI GGCGGA 1 cut(s) 323
Eco130I CCWWGG 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 186
Eco57I CTGAAG 2 cut(s) 75, 207
Eco88I CYCGRG 1 cut(s) 18
Eco91I GGTNACC 1 cut(s) 283
EcoO65I GGTNACC 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 100, 430
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaiI YATR 3 cut(s) 231, 323, 408
FaqI GGGAC 1 cut(s) 415
Fnu4HI GCNGC 1 cut(s) 158
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 158
FspBI CTAG 1 cut(s) 245
GluI GCNGC 1 cut(s) 158
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 1 cut(s) 429
HapII CCGG 1 cut(s) 134
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 1 cut(s) 206
HpaII CCGG 1 cut(s) 134
HphI GGTGA 2 cut(s) 41, 422
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 6 cut(s) 211, 226, 259, 274, 304, 394
Hpy188III TCNNGA 7 cut(s) 119, 134, 170, 188, 245, 263, 418
Hpy8I GTNNAC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 177, 424
HpyCH4V TGCA 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 365, 387
HpyF3I CTNAG 2 cut(s) 208, 303
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 3 cut(s) 211, 259, 292
LmnI GCTCC 2 cut(s) 136, 237
Lsp1109I GCAGC 1 cut(s) 144
LweI GCATC 2 cut(s) 368, 368
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 2 cut(s) 283, 410
MalI GATC 3 cut(s) 213, 261, 294
MboI GATC 3 cut(s) 211, 259, 292
MboII GAAGA 3 cut(s) 78, 81, 213
MflI RGATCY 2 cut(s) 211, 259
MluCI AATT 2 cut(s) 201, 350
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 7 cut(s) 106, 141, 177, 195, 274, 298, 349
MroI TCCGGA 1 cut(s) 133
MseI TTAA 1 cut(s) 333
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 2 cut(s) 102, 432
MvaI CCWGG 2 cut(s) 102, 432
MwoI GCNNNNNNNGC 3 cut(s) 157, 365, 387
NdeII GATC 3 cut(s) 211, 259, 292
NmuCI GTSAC 1 cut(s) 410
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 1 cut(s) 159
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 2 cut(s) 100, 430
PspEI GGTNACC 1 cut(s) 283
PspGI CCWGG 2 cut(s) 100, 430
PspPI GGNCC 1 cut(s) 428
PstI CTGCAG 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 107
PsuI RGATCY 2 cut(s) 211, 259
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 1 cut(s) 158
Sau3AI GATC 3 cut(s) 211, 259, 292
Sau96I GGNCC 1 cut(s) 428
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 102, 432
SetI ASST 7 cut(s) 63, 97, 159, 242, 272, 285, 392
SfaNI GCATC 2 cut(s) 368, 368
SfcI CTRYAG 1 cut(s) 158
SmlI CTYRAG 2 cut(s) 62, 186
SmoI CTYRAG 2 cut(s) 62, 186
Sse9I AATT 2 cut(s) 201, 350
SsiI CCGC 1 cut(s) 308
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 2 cut(s) 100, 430
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 177, 424
TaqI TCGA 2 cut(s) 264, 291
TasI AATT 2 cut(s) 201, 350
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 2 cut(s) 182, 369
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 1 cut(s) 157
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 1 cut(s) 363
TspRI CASTG 2 cut(s) 182, 369
Van91I CCANNNNNTGG 1 cut(s) 107
XbaI TCTAGA 1 cut(s) 244
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.