Rmu_sc0003413.1_g000021

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003413.1
OG0019336
Physical Location & Seq
Reverse (-)
94191 .. 97970
3780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003413.1_g000021.1.cds

Sequence Viewer

Length: 468 bp
atgtttacatatcctgttaaggatgggaatacatctcgggtagatggtgatgatgttcactgggatactgatgatgagcttgagattgaaaacttcactttatcttcttcagctaatccaggctctggggaggctcttgaaagtagtcctccggagcaacattctctgccagagctgcagtctcctccagaacagtgctctcctcaagaacagcaacaaattgactcagatctcttttcttcagattatgatgggagctttctagatggtttttcagatctcgatagctctgatgatgaggtaaccatcgatcctacatctgatgcggagagaaaacttatgtccttggttaaaatggggtacaatgaattggaggcatcagtggccatagaaagatgcgatgtcagctcggaaactgggacttatggtgacttcctgacagttggccctggcattgagaactcgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

155

Amino Acids

16.89

Weight (kDa)

4.05

Isoelectric Point (pI)

61.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019336)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0321571 RchiOBHm_Chr1g0328931 RchiOBHm_Chr2g0172481
rosa_multiflora Rmu_sc0003413.1_g000021 Rmu_sc0033684.1_g000002
rosa_roxburghii Rroxscaffold_2G00115020
rosa_samantha Rh1AG052900 Rh3AG281200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 125
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 305
AcoI YGGCCR 1 cut(s) 384
AcuI CTGAAG 2 cut(s) 93, 225
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 1 cut(s) 125
AgsI TTSAA 2 cut(s) 89, 140
AjnI CCWGG 2 cut(s) 118, 448
AluBI AGCT 6 cut(s) 79, 113, 175, 258, 288, 408
AluI AGCT 6 cut(s) 79, 113, 175, 258, 288, 408
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 186
AlwI GGATC 1 cut(s) 305
AlwNI CAGNNNCTG 1 cut(s) 125
Ama87I CYCGRG 1 cut(s) 36
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 2 cut(s) 384, 445
ApeKI GCWGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 446
AsuHPI GGTGA 2 cut(s) 59, 440
AvaI CYCGRG 1 cut(s) 36
BalI TGGCCA 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 162
BccI CCATC 5 cut(s) 17, 38, 245, 260, 314
BciT130I CCWGG 2 cut(s) 120, 450
BciVI GTATCC 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 176
BfuI GTATCC 1 cut(s) 58
BglII AGATCT 2 cut(s) 229, 277
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme1390I CCNGG 2 cut(s) 120, 450
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 446
BmrFI CCNGG 2 cut(s) 120, 450
BmrI ACTGGG 2 cut(s) 70, 426
BmsI GCATC 3 cut(s) 313, 386, 386
BmuI ACTGGG 2 cut(s) 70, 426
BpmI CTGGAG 1 cut(s) 171
BpuEI CTTGAG 2 cut(s) 101, 189
Bsa29I ATCGAT 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 345, 448
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 65, 421
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 2 cut(s) 120, 450
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 345, 448
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 1 cut(s) 125
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 2 cut(s) 65, 421
BseRI GAGGAG 2 cut(s) 174, 192
BseXI GCAGC 1 cut(s) 162
BshFI GGCC 2 cut(s) 386, 447
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 1 cut(s) 125
BsmAI GTCTC 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 433
BsnI GGCC 2 cut(s) 386, 447
BsoBI CYCGRG 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 200
Bsp13I TCCGGA 1 cut(s) 151
Bsp143I GATC 3 cut(s) 229, 277, 310
BspACI CCGC 1 cut(s) 326
BspANI GGCC 2 cut(s) 386, 447
BspCNI CTCAG 1 cut(s) 239
BspDI ATCGAT 1 cut(s) 309
BspEI TCCGGA 1 cut(s) 151
BspMAI CTGCAG 1 cut(s) 180
BspPI GGATC 1 cut(s) 305
BsrI ACTGG 2 cut(s) 65, 421
BssECI CCNNGG 2 cut(s) 345, 448
BssMI GATC 3 cut(s) 229, 277, 310
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 2 cut(s) 120, 450
Bst4CI ACNGT 2 cut(s) 195, 442
BstDEI CTNAG 1 cut(s) 226
BstEII GGTNACC 1 cut(s) 301
BstF5I GGATG 1 cut(s) 28
BstKTI GATC 3 cut(s) 232, 280, 313
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 3 cut(s) 229, 277, 310
BstMWI GCNNNNNNNGC 3 cut(s) 175, 383, 405
BstNI CCWGG 2 cut(s) 120, 450
BstPI GGTNACC 1 cut(s) 301
BstSCI CCNGG 2 cut(s) 118, 448
BstSFI CTRYAG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 162
BstX2I RGATCY 2 cut(s) 229, 277
BstYI RGATCY 2 cut(s) 229, 277
Bsu15I ATCGAT 1 cut(s) 309
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 2 cut(s) 386, 447
BsuTUI ATCGAT 1 cut(s) 309
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 1 cut(s) 28
BtsIMutI CAGTG 3 cut(s) 58, 200, 387
CaiI CAGNNNCTG 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 446
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 1 cut(s) 361
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 1 cut(s) 226
DpnI GATC 3 cut(s) 231, 279, 312
DpnII GATC 3 cut(s) 229, 277, 310
EaeI YGGCCR 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 345
Eco57I CTGAAG 2 cut(s) 93, 225
Eco88I CYCGRG 1 cut(s) 36
Eco91I GGTNACC 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 301
EcoRII CCWGG 2 cut(s) 118, 448
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaiI YATR 5 cut(s) 10, 249, 341, 389, 426
FaqI GGGAC 1 cut(s) 433
Fnu4HI GCNGC 1 cut(s) 176
FokI GGATG 1 cut(s) 35
Fsp4HI GCNGC 1 cut(s) 176
FspBI CTAG 1 cut(s) 263
GluI GCNGC 1 cut(s) 176
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 386, 447
HapII CCGG 1 cut(s) 152
HinfI GANTC 1 cut(s) 224
HpaII CCGG 1 cut(s) 152
HphI GGTGA 2 cut(s) 59, 440
Hpy166II GTNNAC 2 cut(s) 6, 58
Hpy188I TCNGA 6 cut(s) 229, 244, 277, 292, 322, 412
Hpy188III TCNNGA 7 cut(s) 137, 152, 188, 206, 263, 281, 436
Hpy8I GTNNAC 2 cut(s) 6, 58
HpyCH4III ACNGT 2 cut(s) 195, 442
HpyCH4V TGCA 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 383, 405
HpyF3I CTNAG 1 cut(s) 226
Kpn2I TCCGGA 1 cut(s) 151
Kzo9I GATC 3 cut(s) 229, 277, 310
LmnI GCTCC 2 cut(s) 154, 255
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 3 cut(s) 313, 386, 386
MaeI CTAG 1 cut(s) 263
MaeIII GTNAC 2 cut(s) 301, 428
MalI GATC 3 cut(s) 231, 279, 312
MboI GATC 3 cut(s) 229, 277, 310
MboII GAAGA 3 cut(s) 96, 99, 231
MflI RGATCY 2 cut(s) 229, 277
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 2 cut(s) 219, 368
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 6 cut(s) 124, 159, 195, 213, 292, 367
Mox20I TGGCCA 1 cut(s) 386
MroI TCCGGA 1 cut(s) 151
MscI TGGCCA 1 cut(s) 386
MseI TTAA 2 cut(s) 18, 351
Msp20I TGGCCA 1 cut(s) 386
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 2 cut(s) 120, 450
MvaI CCWGG 2 cut(s) 120, 450
MwoI GCNNNNNNNGC 3 cut(s) 175, 383, 405
NdeII GATC 3 cut(s) 229, 277, 310
NmuCI GTSAC 1 cut(s) 428
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 1 cut(s) 177
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp6I CCWGG 2 cut(s) 118, 448
PspEI GGTNACC 1 cut(s) 301
PspGI CCWGG 2 cut(s) 118, 448
PspPI GGNCC 1 cut(s) 446
PstI CTGCAG 1 cut(s) 180
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 2 cut(s) 229, 277
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SaqAI TTAA 2 cut(s) 18, 351
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 3 cut(s) 229, 277, 310
Sau96I GGNCC 1 cut(s) 446
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 2 cut(s) 120, 450
SduI GDGCHC 1 cut(s) 200
SetI ASST 7 cut(s) 81, 115, 177, 260, 290, 303, 410
SfaNI GCATC 3 cut(s) 313, 386, 386
SfcI CTRYAG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 80, 204
SmoI CTYRAG 2 cut(s) 80, 204
Sse9I AATT 2 cut(s) 219, 368
SsiI CCGC 1 cut(s) 326
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 118, 448
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 195, 442
TaqI TCGA 2 cut(s) 282, 309
TasI AATT 2 cut(s) 219, 368
Tru1I TTAA 2 cut(s) 18, 351
Tru9I TTAA 2 cut(s) 18, 351
TscAI CASTG 3 cut(s) 65, 200, 387
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 1 cut(s) 381
TspRI CASTG 3 cut(s) 65, 200, 387
Van91I CCANNNNNTGG 1 cut(s) 125
XbaI TCTAGA 1 cut(s) 262
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.