RchiOBHm_Chr1g0337361

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0014792
Physical Location & Seq
Reverse (-)
29015662 .. 29022923
7262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGGAAGATGAATGGAAAATTACTGAGGCCATGCCACAGCTCATGGTGGCTTCAGAAAAAGCATTTGAAGCTGCAGATTTCTATGAACTCTTATTTGGAGCACAGAAAACTCGATACAATGAGTTTCCCATGCAGAGTCGAACGTCTGACAGGTACTGCCGGGTTGCATGTGTGGAGCTCACAATAGGTTCCAGCCCTCTTGTGGTGAGGGATTTTGACAGTATAAACAGTAGGGTTGTGAGAGATGTAAATTCATGGGTTTCCACTTCCCTCTGCCTTCAATGCTTGAACCTGGACCTTATGATGGCAAGGGCTGTCAGTATAGGCTGCACAGTTCTTAAAGAAATTTTGGAGGATCCAGTTCGTCCTGGTGTGATGGTCGGCATTATAAGAGATCCATTTGGTCATGTATGGGAGCTCAGCAACTCATCTTATGGTGTCTCAGGAGAGGGTTTCATGAACACTGAGTGCATGAACGTGTTGCTTCAAGGTTGGAGCAAGAGTAAGAGGGAGGAGAACAAAGGGCTGTTTGAAACCTATGATTTCACACCAATGGTTGGGTCCTGTGAGACAAAGAAGAATGCAGCACATATAGTCAGTACGTGCAGACATCAAGGTCCCTTATTTGACCCCATTCTTTTTTATTGCAAATACATGGTAAGCCATCATGGAATCTTATGTATCTTGAAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

231

Amino Acids

26.14

Weight (kDa)

5.59

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 13 - 71 1.8e-06 Glyoxalase At5g48480-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AasI GACNNNNNNGTC 1 cut(s) 615
AccB7I CCANNNNNTGG 1 cut(s) 557
AclWI GGATC 3 cut(s) 350, 363, 389
AcsI RAATTY 2 cut(s) 250, 345
AcuI CTGAAG 1 cut(s) 36
AdeI CACNNNGTG 1 cut(s) 468
AfaI GTAC 2 cut(s) 155, 601
AfiI CCNNNNNNNGG 3 cut(s) 202, 304, 557
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 6 cut(s) 68, 281, 289, 488, 533, 688
AjnI CCWGG 2 cut(s) 291, 367
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 4 cut(s) 40, 71, 178, 418
AluI AGCT 4 cut(s) 40, 71, 178, 418
Alw21I GWGCWC 3 cut(s) 103, 180, 420
Alw26I GTCTC 2 cut(s) 445, 563
AlwI GGATC 3 cut(s) 350, 363, 389
AlwNI CAGNNNCTG 1 cut(s) 156
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 3 cut(s) 71, 327, 584
ApoI RAATTY 2 cut(s) 250, 345
AspS9I GGNCC 3 cut(s) 295, 561, 617
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 3 cut(s) 295, 561, 617
BamHI GGATCC 1 cut(s) 355
BanII GRGCYC 2 cut(s) 180, 420
Bbv12I GWGCWC 3 cut(s) 103, 180, 420
BbvI GCAGC 3 cut(s) 58, 314, 596
BccI CCATC 3 cut(s) 298, 370, 672
BciT130I CCWGG 2 cut(s) 293, 369
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 2 cut(s) 445, 563
BfmI CTRYAG 1 cut(s) 72
BisI GCNGC 3 cut(s) 72, 328, 585
BlpI GCTNAGC 1 cut(s) 419
BlsI GCNGC 3 cut(s) 73, 329, 586
Bme1390I CCNGG 3 cut(s) 161, 293, 369
Bme18I GGWCC 3 cut(s) 295, 561, 617
BmgT120I GGNCC 3 cut(s) 295, 561, 617
BmiI GGNNCC 4 cut(s) 190, 357, 562, 619
BmrFI CCNGG 3 cut(s) 161, 293, 369
Bpu1102I GCTNAGC 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 603
BsaXI ACNNNNNCTCC 2 cut(s) 487, 517
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 304, 557
Bse1I ACTGG 1 cut(s) 359
BseBI CCWGG 2 cut(s) 293, 369
BseLI CCNNNNNNNGG 3 cut(s) 202, 304, 557
BseMII CTCAG 4 cut(s) 15, 433, 456, 456
BseNI ACTGG 1 cut(s) 359
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 3 cut(s) 58, 314, 596
BsgI GTGCAG 2 cut(s) 313, 625
BshFI GGCC 1 cut(s) 29
BsiHKAI GWGCWC 3 cut(s) 103, 180, 420
BsiSI CCGG 1 cut(s) 160
BslFI GGGAC 1 cut(s) 603
BslI CCNNNNNNNGG 3 cut(s) 202, 304, 557
BsmAI GTCTC 2 cut(s) 445, 563
BsmFI GGGAC 1 cut(s) 603
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 3 cut(s) 103, 180, 420
Bsp143I GATC 2 cut(s) 355, 394
Bsp1720I GCTNAGC 1 cut(s) 419
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 4 cut(s) 16, 432, 455, 457
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 4 cut(s) 190, 357, 562, 619
BspMAI CTGCAG 1 cut(s) 76
BspPI GGATC 3 cut(s) 350, 363, 389
BsrI ACTGG 1 cut(s) 359
BssMI GATC 2 cut(s) 355, 394
Bst2UI CCWGG 2 cut(s) 293, 369
Bst4CI ACNGT 3 cut(s) 221, 230, 334
BstBAI YACGTR 1 cut(s) 603
BstDEI CTNAG 4 cut(s) 24, 419, 442, 465
BstKTI GATC 2 cut(s) 358, 397
BstMAI GTCTC 2 cut(s) 445, 563
BstMBI GATC 2 cut(s) 355, 394
BstMWI GCNNNNNNNGC 2 cut(s) 68, 282
BstNI CCWGG 2 cut(s) 293, 369
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 3 cut(s) 159, 291, 367
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 3 cut(s) 58, 314, 596
BstX2I RGATCY 2 cut(s) 355, 394
BstYI RGATCY 2 cut(s) 355, 394
BsuRI GGCC 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 462
CaiI CAGNNNCTG 1 cut(s) 156
CciI TCATGA 1 cut(s) 456
Cfr13I GGNCC 3 cut(s) 295, 561, 617
Csp6I GTAC 2 cut(s) 154, 600
CviQI GTAC 2 cut(s) 154, 600
DdeI CTNAG 4 cut(s) 24, 419, 442, 465
DpnI GATC 2 cut(s) 357, 396
DpnII GATC 2 cut(s) 355, 394
DraIII CACNNNGTG 1 cut(s) 468
DrdI GACNNNNNNGTC 1 cut(s) 615
DseDI GACNNNNNNGTC 1 cut(s) 615
Ecl136II GAGCTC 2 cut(s) 178, 418
Eco24I GRGCYC 2 cut(s) 180, 420
Eco47I GGWCC 3 cut(s) 295, 561, 617
Eco53kI GAGCTC 2 cut(s) 178, 418
Eco57I CTGAAG 1 cut(s) 36
EcoICRI GAGCTC 2 cut(s) 178, 418
EcoO109I RGGNCCY 2 cut(s) 561, 617
EcoRII CCWGG 2 cut(s) 291, 367
EcoT38I GRGCYC 2 cut(s) 180, 420
FaqI GGGAC 1 cut(s) 603
Fnu4HI GCNGC 3 cut(s) 72, 328, 585
FriOI GRGCYC 2 cut(s) 180, 420
Fsp4HI GCNGC 3 cut(s) 72, 328, 585
GluI GCNGC 3 cut(s) 72, 328, 585
HaeIII GGCC 1 cut(s) 29
HapII CCGG 1 cut(s) 160
HinfI GANTC 2 cut(s) 136, 672
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 217
Hpy188I TCNGA 2 cut(s) 55, 148
Hpy188III TCNNGA 3 cut(s) 444, 457, 685
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 3 cut(s) 221, 230, 334
HpyCH4IV ACGT 3 cut(s) 143, 477, 602
HpyCH4V TGCA 8 cut(s) 74, 133, 167, 330, 471, 584, 606, 648
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 282
HpyF3I CTNAG 4 cut(s) 24, 419, 442, 465
HpySE526I ACGT 3 cut(s) 143, 477, 602
Kzo9I GATC 2 cut(s) 355, 394
LmnI GCTCC 4 cut(s) 98, 175, 415, 495
Lsp1109I GCAGC 3 cut(s) 58, 314, 596
MaeII ACGT 3 cut(s) 143, 477, 602
MalI GATC 2 cut(s) 357, 396
MboI GATC 2 cut(s) 355, 394
MboII GAAGA 2 cut(s) 17, 589
MflI RGATCY 2 cut(s) 355, 394
MhlI GDGCHC 3 cut(s) 103, 180, 420
MluCI AATT 3 cut(s) 18, 250, 345
MlyI GAGTC 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 473
MnlI CCTC 8 cut(s) 19, 201, 207, 281, 346, 442, 501, 505
MseI TTAA 2 cut(s) 339, 694
MslI CAYNNNNRTG 2 cut(s) 476, 551
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 3 cut(s) 161, 293, 369
Mva1269I GAATGC 1 cut(s) 586
MvaI CCWGG 2 cut(s) 293, 369
MwoI GCNNNNNNNGC 2 cut(s) 68, 282
NciI CCSGG 1 cut(s) 161
NdeII GATC 2 cut(s) 355, 394
NlaIV GGNNCC 4 cut(s) 190, 357, 562, 619
NspI RCATGY 1 cut(s) 171
PagI TCATGA 1 cut(s) 456
PctI GAATGC 1 cut(s) 586
PfeI GAWTC 1 cut(s) 672
PflMI CCANNNNNTGG 1 cut(s) 557
PkrI GCNGC 3 cut(s) 73, 329, 586
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
Ppu21I YACGTR 1 cut(s) 603
PpuMI RGGWCCY 2 cut(s) 561, 617
PsiI TTATAA 1 cut(s) 389
Psp124BI GAGCTC 2 cut(s) 180, 420
Psp5II RGGWCCY 2 cut(s) 561, 617
Psp6I CCWGG 2 cut(s) 291, 367
PspGI CCWGG 2 cut(s) 291, 367
PspN4I GGNNCC 4 cut(s) 190, 357, 562, 619
PspPI GGNCC 3 cut(s) 295, 561, 617
PspPPI RGGWCCY 2 cut(s) 561, 617
PstI CTGCAG 1 cut(s) 76
PstNI CAGNNNCTG 1 cut(s) 156
PsuI RGATCY 2 cut(s) 355, 394
RsaI GTAC 2 cut(s) 155, 601
RsaNI GTAC 2 cut(s) 154, 600
RseI CAYNNNNRTG 2 cut(s) 476, 551
SacI GAGCTC 2 cut(s) 180, 420
SaqAI TTAA 2 cut(s) 339, 694
SatI GCNGC 3 cut(s) 72, 328, 585
Sau3AI GATC 2 cut(s) 355, 394
Sau96I GGNCC 3 cut(s) 295, 561, 617
SchI GAGTC 1 cut(s) 145
ScrFI CCNGG 3 cut(s) 161, 293, 369
SduI GDGCHC 3 cut(s) 103, 180, 420
SfcI CTRYAG 1 cut(s) 72
SinI GGWCC 3 cut(s) 295, 561, 617
SmiMI CAYNNNNRTG 2 cut(s) 476, 551
Sse9I AATT 3 cut(s) 18, 250, 345
SstI GAGCTC 2 cut(s) 180, 420
StyD4I CCNGG 3 cut(s) 159, 291, 367
TaaI ACNGT 3 cut(s) 221, 230, 334
TaiI ACGT 3 cut(s) 146, 480, 605
TaqI TCGA 2 cut(s) 112, 139
TasI AATT 3 cut(s) 18, 250, 345
TfiI GAWTC 1 cut(s) 672
Tru1I TTAA 2 cut(s) 339, 694
Tru9I TTAA 2 cut(s) 339, 694
TscAI CASTG 1 cut(s) 469
TseI GCWGC 3 cut(s) 71, 327, 584
TspDTI ATGAA 6 cut(s) 24, 99, 243, 445, 473, 488
TspRI CASTG 1 cut(s) 469
Van91I CCANNNNNTGG 1 cut(s) 557
VpaK11BI GGWCC 3 cut(s) 295, 561, 617
XapI RAATTY 2 cut(s) 250, 345
XceI RCATGY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.