RchiOBHm_Chr1g0339791

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0019660
Physical Location & Seq
Forward (+)
31224754 .. 31225837
1084 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGGCTCCAAGTAGGACCGTGCAAACCACCACCCCCTCCGCTAACCCTCCCATCCCTCCTCTTATTGACCCACCACCTCCCCACCAACCTGCCATTACTCAACTAGCTTCTTCCTCATCCCAACCTGATGACCTTCAACACCTCCAGATGGTTGAGTTTCAGCGCTCCATCGACCTCTTGCAAGACGAACTTCATCAGTCCGTCGACTCCATCAATCAAAATCAATTTGCTTTCCAGAACCATATCGCTGCCCAAATCGCGGCGCTTCAAAGCACGTTGGTGCATGCCTTAACCAACCGACCACAACCATTGCCCTCTCCCCCACCTCCAGCCTCTTTGCAATTCCCTTTACCACCAACACCTTCCACTGTCCCAGCCATCACCTTTGGCTCCATTCCTTCACCACATACACCTCCACACACAGCCGCTCCCCTTCACGACTCCACAATCATGTCCATACCCCAGAGCATCCCCACCCCTGATGTACACACCCCATTTCCTACCTTTAATGCCGCCCACCGACCACTGCACCACTATTATAAGGAACACCAAACCCTTCGCCCACCCAAGTTTAACCTCCCTATCTTCACTGGAGACGACGTAATTGGTTGGTTCACCATGGCTGAGCGGTATCTCCGTACACAAAGAATTCCCCCCTCTGAGCACATCTTCACCGTAGCCTCTCATTTCGGCCCTGATGCATGCATGTGGATGAATTCATTCGAGCAACAATTCCCGACAGCCACTTGGGACAAATTTGCCTCCGCGTTTCTGGAGCATCACGGGGCCGGCAACACGATGGAATTCAAAATAGCCCTTTCCAATCTGAAACAGTCAGCCTCCGTGAATGAATTCATCTTAACCTTTACAAAGCTTGCTGGCAGAACAAAAGGGTGGTCGGAGGCCGAGTTGCTACCCATATTTCTCAGCGGGTTGAAACCGGAGATCCAACATGATGTGATTGCATTGGACCCCCAAACACTGGTCTCTGCTCAGCGCCTGGCACGCCGCTTTGAGCTCAAGCTGGCCGACATCCGCTCCTCACGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

349

Amino Acids

38.77

Weight (kDa)

6.4

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 215 - 336 2e-12 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 227 - 314 1e-10 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06281 FvH4_4g00101
rosa_chinensis RchiOBHm_Chr1g0339791 RchiOBHm_Chr4g0426521
rosa_multiflora Rmu_sc0007466.1_g000003
rosa_samantha Rh6CG335600
rosa_wichuraiana Rw0G010860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 97
AccB7I CCANNNNNTGG 1 cut(s) 982
AccBSI CCGCTC 3 cut(s) 428, 628, 1038
AccI GTMKAC 1 cut(s) 204
AccII CGCG 2 cut(s) 260, 767
AciI CCGC 9 cut(s) 39, 260, 426, 513, 628, 765, 930, 1009, 1036
AclWI GGATC 1 cut(s) 940
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 5 cut(s) 648, 715, 755, 803, 851
AfaI GTAC 2 cut(s) 486, 640
AfeI AGCGCT 1 cut(s) 164
AfiI CCNNNNNNNGG 3 cut(s) 148, 259, 982
AgsI TTSAA 4 cut(s) 137, 269, 808, 937
AjnI CCWGG 1 cut(s) 999
AleI CACNNNNGTG 1 cut(s) 278
AluBI AGCT 4 cut(s) 107, 874, 1018, 1024
AluI AGCT 4 cut(s) 107, 874, 1018, 1024
Alw21I GWGCWC 2 cut(s) 666, 1020
Alw26I GTCTC 2 cut(s) 588, 991
AlwI GGATC 1 cut(s) 940
AlwNI CAGNNNCTG 1 cut(s) 1000
Aor51HI AGCGCT 1 cut(s) 164
AoxI GGCC 4 cut(s) 691, 786, 903, 1026
ApeKI GCWGC 1 cut(s) 248
ApoI RAATTY 5 cut(s) 648, 715, 755, 803, 851
ArsI GACNNNNNNTTYG 2 cut(s) 881, 913
Asp700I GAANNNNTTC 2 cut(s) 719, 851
AspLEI GCGC 3 cut(s) 165, 265, 999
AspS9I GGNCC 4 cut(s) 15, 692, 786, 970
AsuHPI GGTGA 4 cut(s) 373, 393, 607, 664
AvaII GGWCC 2 cut(s) 15, 970
BanII GRGCYC 1 cut(s) 1020
Bbv12I GWGCWC 2 cut(s) 666, 1020
BbvI GCAGC 1 cut(s) 235
BccI CCATC 6 cut(s) 59, 142, 176, 218, 386, 793
BciT130I CCWGG 1 cut(s) 1001
BcoDI GTCTC 2 cut(s) 588, 991
BfaI CTAG 1 cut(s) 104
BfoI RGCGCY 3 cut(s) 166, 266, 1000
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 5 cut(s) 249, 261, 426, 513, 1009
BlpI GCTNAGC 2 cut(s) 624, 993
BlsI GCNGC 5 cut(s) 250, 262, 427, 514, 1010
Bme1390I CCNGG 1 cut(s) 1001
Bme18I GGWCC 2 cut(s) 15, 970
BmgT120I GGNCC 4 cut(s) 15, 692, 786, 970
BmiI GGNNCC 4 cut(s) 6, 391, 787, 972
BmrFI CCNGG 1 cut(s) 1001
BmsI GCATC 3 cut(s) 477, 688, 787
BpmI CTGGAG 4 cut(s) 128, 312, 612, 794
Bpu1102I GCTNAGC 2 cut(s) 624, 993
BpuEI CTTGAG 1 cut(s) 1004
BsaI GGTCTC 1 cut(s) 991
BsaJI CCNNGG 1 cut(s) 618
BsaWI WCCGGW 1 cut(s) 940
BsaXI ACNNNNNCTCC 7 cut(s) 412, 442, 585, 615, 893, 923, 1022
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 259, 982
Bse118I RCCGGY 1 cut(s) 788
Bse1I ACTGG 2 cut(s) 595, 987
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 1 cut(s) 1001
BseDI CCNNGG 1 cut(s) 618
BseGI GGATG 5 cut(s) 51, 116, 468, 717, 1032
BseLI CCNNNNNNNGG 3 cut(s) 148, 259, 982
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 4 cut(s) 615, 651, 940, 1007
BseNI ACTGG 2 cut(s) 595, 987
BseRI GAGGAG 2 cut(s) 48, 1030
BseXI GCAGC 1 cut(s) 235
BseYI CCCAGC 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 512
Bsh1236I CGCG 2 cut(s) 260, 767
BshFI GGCC 4 cut(s) 693, 788, 905, 1028
BsiHKAI GWGCWC 2 cut(s) 666, 1020
BsiSI CCGG 2 cut(s) 789, 941
BslFI GGGAC 2 cut(s) 356, 764
BslI CCNNNNNNNGG 3 cut(s) 148, 259, 982
BsmAI GTCTC 2 cut(s) 588, 991
BsmBI CGTCTC 1 cut(s) 588
BsmFI GGGAC 2 cut(s) 356, 764
BsnI GGCC 4 cut(s) 693, 788, 905, 1028
Bso31I GGTCTC 1 cut(s) 991
Bsp1286I GDGCHC 2 cut(s) 666, 1020
Bsp1407I TGTACA 1 cut(s) 484
Bsp143I GATC 1 cut(s) 945
Bsp1720I GCTNAGC 2 cut(s) 624, 993
Bsp19I CCATGG 1 cut(s) 618
BspACI CCGC 9 cut(s) 39, 260, 426, 513, 628, 765, 930, 1009, 1036
BspANI GGCC 4 cut(s) 693, 788, 905, 1028
BspCNI CTCAG 4 cut(s) 616, 652, 939, 1006
BspFNI CGCG 2 cut(s) 260, 767
BspLI GGNNCC 4 cut(s) 6, 391, 787, 972
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 1 cut(s) 940
BspTNI GGTCTC 1 cut(s) 991
BsrBI CCGCTC 3 cut(s) 428, 628, 1038
BsrDI GCAATG 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 788
BsrGI TGTACA 1 cut(s) 484
BsrI ACTGG 2 cut(s) 595, 987
BssAI RCCGGY 1 cut(s) 788
BssECI CCNNGG 1 cut(s) 618
BssMI GATC 1 cut(s) 945
BssT1I CCWWGG 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 1001
Bst4CI ACNGT 4 cut(s) 19, 370, 676, 834
BstAUI TGTACA 1 cut(s) 484
BstC8I GCNNGC 7 cut(s) 285, 703, 790, 876, 880, 1006, 1026
BstDEI CTNAG 4 cut(s) 624, 660, 926, 993
BstDSI CCRYGG 1 cut(s) 618
BstF5I GGATG 5 cut(s) 51, 116, 468, 717, 1032
BstFNI CGCG 2 cut(s) 260, 767
BstH2I RGCGCY 3 cut(s) 166, 266, 1000
BstHHI GCGC 3 cut(s) 165, 265, 999
BstKTI GATC 1 cut(s) 948
BstMAI GTCTC 2 cut(s) 588, 991
BstMBI GATC 1 cut(s) 945
BstMWI GCNNNNNNNGC 2 cut(s) 257, 1005
BstNI CCWGG 1 cut(s) 1001
BstNSI RCATGY 3 cut(s) 287, 705, 709
BstSCI CCNGG 1 cut(s) 999
BstUI CGCG 2 cut(s) 260, 767
BstV1I GCAGC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 945
BstYI RGATCY 1 cut(s) 945
BsuRI GGCC 4 cut(s) 693, 788, 905, 1028
BtgI CCRYGG 1 cut(s) 618
BtsCI GGATG 5 cut(s) 51, 116, 468, 717, 1032
BtsI GCAGTG 1 cut(s) 524
BtsIMutI CAGTG 4 cut(s) 366, 524, 588, 980
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 7 cut(s) 285, 703, 790, 876, 880, 1006, 1026
CaiI CAGNNNCTG 1 cut(s) 1000
CfoI GCGC 3 cut(s) 165, 265, 999
Cfr10I RCCGGY 1 cut(s) 788
Cfr13I GGNCC 4 cut(s) 15, 692, 786, 970
Csp6I GTAC 2 cut(s) 485, 639
CspCI CAANNNNNGTGG 2 cut(s) 291, 326
CviAII CATG 6 cut(s) 284, 451, 619, 702, 706, 953
CviQI GTAC 2 cut(s) 485, 639
DdeI CTNAG 4 cut(s) 624, 660, 926, 993
DpnI GATC 1 cut(s) 947
DpnII GATC 1 cut(s) 945
EaeI YGGCCR 1 cut(s) 1026
Ecl136II GAGCTC 1 cut(s) 1018
Eco130I CCWWGG 1 cut(s) 618
Eco24I GRGCYC 1 cut(s) 1020
Eco31I GGTCTC 1 cut(s) 991
Eco47I GGWCC 2 cut(s) 15, 970
Eco47III AGCGCT 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 1018
EcoICRI GAGCTC 1 cut(s) 1018
EcoRI GAATTC 4 cut(s) 648, 715, 803, 851
EcoRII CCWGG 1 cut(s) 999
EcoT14I CCWWGG 1 cut(s) 618
EcoT22I ATGCAT 2 cut(s) 703, 707
EcoT38I GRGCYC 1 cut(s) 1020
ErhI CCWWGG 1 cut(s) 618
Esp3I CGTCTC 1 cut(s) 588
FaeI CATG 6 cut(s) 287, 454, 622, 705, 709, 956
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 2 cut(s) 356, 764
FatI CATG 6 cut(s) 283, 450, 618, 701, 705, 952
FauI CCCGC 1 cut(s) 923
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 5 cut(s) 249, 261, 426, 513, 1009
FokI GGATG 5 cut(s) 38, 103, 455, 724, 1019
FriOI GRGCYC 1 cut(s) 1020
Fsp4HI GCNGC 5 cut(s) 249, 261, 426, 513, 1009
FspBI CTAG 1 cut(s) 104
GlaI GCGC 3 cut(s) 164, 264, 998
GluI GCNGC 5 cut(s) 249, 261, 426, 513, 1009
GsaI CCCAGC 1 cut(s) 377
GsuI CTGGAG 4 cut(s) 128, 312, 612, 794
HaeII RGCGCY 3 cut(s) 166, 266, 1000
HaeIII GGCC 4 cut(s) 693, 788, 905, 1028
HapII CCGG 2 cut(s) 789, 941
HhaI GCGC 3 cut(s) 165, 265, 999
Hin1II CATG 6 cut(s) 287, 454, 622, 705, 709, 956
Hin6I GCGC 3 cut(s) 163, 263, 997
HinP1I GCGC 3 cut(s) 163, 263, 997
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 2 cut(s) 206, 440
HpaII CCGG 2 cut(s) 789, 941
HphI GGTGA 4 cut(s) 373, 393, 607, 664
Hpy166II GTNNAC 4 cut(s) 205, 487, 615, 641
Hpy188I TCNGA 3 cut(s) 661, 828, 901
Hpy188III TCNNGA 5 cut(s) 145, 235, 437, 736, 773
Hpy8I GTNNAC 4 cut(s) 205, 487, 615, 641
Hpy99I CGWCG 2 cut(s) 206, 602
HpyAV CCTTC 5 cut(s) 143, 372, 408, 443, 566
HpyCH4III ACNGT 4 cut(s) 19, 370, 676, 834
HpyCH4IV ACGT 3 cut(s) 275, 600, 1045
HpyCH4V TGCA 8 cut(s) 22, 181, 283, 340, 529, 701, 705, 965
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 1005
HpyF3I CTNAG 4 cut(s) 624, 660, 926, 993
HpySE526I ACGT 3 cut(s) 275, 600, 1045
Hsp92II CATG 6 cut(s) 287, 454, 622, 705, 709, 956
HspAI GCGC 3 cut(s) 163, 263, 997
KroI GCCGGC 1 cut(s) 788
KroNI GCCGGC 1 cut(s) 790
Kzo9I GATC 1 cut(s) 945
LmnI GCTCC 6 cut(s) 10, 170, 395, 433, 775, 1043
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 3 cut(s) 477, 688, 787
MaeI CTAG 1 cut(s) 104
MaeII ACGT 3 cut(s) 275, 600, 1045
MalI GATC 1 cut(s) 947
MbiI CCGCTC 3 cut(s) 428, 628, 1038
MboI GATC 1 cut(s) 945
MboII GAAGA 3 cut(s) 102, 577, 661
MflI RGATCY 1 cut(s) 945
MhlI GDGCHC 2 cut(s) 666, 1020
MluCI AATT 9 cut(s) 224, 341, 603, 648, 715, 731, 755, 803, 851
MlyI GAGTC 2 cut(s) 200, 434
MmeI TCCRAC 2 cut(s) 879, 973
Mph1103I ATGCAT 2 cut(s) 703, 707
MroNI GCCGGC 1 cut(s) 788
MroXI GAANNNNTTC 2 cut(s) 719, 851
MseI TTAA 4 cut(s) 290, 507, 573, 860
MslI CAYNNNNRTG 4 cut(s) 278, 449, 706, 710
MspA1I CMGCKG 1 cut(s) 930
MspI CCGG 2 cut(s) 789, 941
MspR9I CCNGG 1 cut(s) 1001
MvaI CCWGG 1 cut(s) 1001
MvnI CGCG 2 cut(s) 260, 767
MwoI GCNNNNNNNGC 2 cut(s) 257, 1005
NaeI GCCGGC 1 cut(s) 790
NcoI CCATGG 1 cut(s) 618
NdeII GATC 1 cut(s) 945
NgoMIV GCCGGC 1 cut(s) 788
NlaIII CATG 6 cut(s) 287, 454, 622, 705, 709, 956
NlaIV GGNNCC 4 cut(s) 6, 391, 787, 972
NmeAIII GCCGAG 1 cut(s) 931
NsiI ATGCAT 2 cut(s) 703, 707
NspI RCATGY 3 cut(s) 287, 705, 709
OliI CACNNNNGTG 1 cut(s) 278
PaeI GCATGC 2 cut(s) 287, 705
PdiI GCCGGC 1 cut(s) 790
PdmI GAANNNNTTC 2 cut(s) 719, 851
PflMI CCANNNNNTGG 1 cut(s) 982
PkrI GCNGC 5 cut(s) 250, 262, 427, 514, 1010
PleI GAGTC 2 cut(s) 200, 434
PpsI GAGTC 2 cut(s) 200, 434
PsiI TTATAA 1 cut(s) 540
Psp124BI GAGCTC 1 cut(s) 1020
Psp6I CCWGG 1 cut(s) 999
PspFI CCCAGC 1 cut(s) 373
PspGI CCWGG 1 cut(s) 999
PspN4I GGNNCC 4 cut(s) 6, 391, 787, 972
PspPI GGNCC 4 cut(s) 15, 692, 786, 970
PstNI CAGNNNCTG 1 cut(s) 1000
PsuI RGATCY 1 cut(s) 945
RsaI GTAC 2 cut(s) 486, 640
RsaNI GTAC 2 cut(s) 485, 639
RseI CAYNNNNRTG 4 cut(s) 278, 449, 706, 710
SacI GAGCTC 1 cut(s) 1020
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 4 cut(s) 290, 507, 573, 860
SatI GCNGC 5 cut(s) 249, 261, 426, 513, 1009
Sau3AI GATC 1 cut(s) 945
Sau96I GGNCC 4 cut(s) 15, 692, 786, 970
SchI GAGTC 2 cut(s) 200, 434
ScrFI CCNGG 1 cut(s) 1001
SduI GDGCHC 2 cut(s) 666, 1020
SfaNI GCATC 3 cut(s) 477, 688, 787
SinI GGWCC 2 cut(s) 15, 970
SmiMI CAYNNNNRTG 4 cut(s) 278, 449, 706, 710
SmlI CTYRAG 1 cut(s) 1019
SmoI CTYRAG 1 cut(s) 1019
SphI GCATGC 2 cut(s) 287, 705
Sse9I AATT 9 cut(s) 224, 341, 603, 648, 715, 731, 755, 803, 851
SsiI CCGC 9 cut(s) 39, 260, 426, 513, 628, 765, 930, 1009, 1036
SspMI CTAG 1 cut(s) 104
SstI GAGCTC 1 cut(s) 1020
StyD4I CCNGG 1 cut(s) 999
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 4 cut(s) 19, 370, 676, 834
TaiI ACGT 3 cut(s) 278, 603, 1048
TaqI TCGA 3 cut(s) 171, 204, 723
TasI AATT 9 cut(s) 224, 341, 603, 648, 715, 731, 755, 803, 851
TatI WGTACW 1 cut(s) 484
TauI GCSGC 4 cut(s) 263, 428, 515, 1011
Tru1I TTAA 4 cut(s) 290, 507, 573, 860
Tru9I TTAA 4 cut(s) 290, 507, 573, 860
TscAI CASTG 4 cut(s) 373, 531, 595, 987
TseI GCWGC 1 cut(s) 248
TspDTI ATGAA 5 cut(s) 182, 708, 728, 844, 864
TspGWI ACGGA 3 cut(s) 190, 626, 832
TspRI CASTG 4 cut(s) 373, 531, 595, 987
Van91I CCANNNNNTGG 1 cut(s) 982
VpaK11BI GGWCC 2 cut(s) 15, 970
XapI RAATTY 5 cut(s) 648, 715, 755, 803, 851
XceI RCATGY 3 cut(s) 287, 705, 709
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 2 cut(s) 719, 851
XspI CTAG 1 cut(s) 104
Zsp2I ATGCAT 2 cut(s) 703, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.