RchiOBHm_Chr1g0341981

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018953
Physical Location & Seq
Reverse (-)
33860219 .. 33861139
921 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGTCCGTCGGTTGCAACACTACTAGTTGCAAAAAGGGTAAAACAGTAATTAAACCCATAATGCCAAAGCTGCCAGCTTTCAATGACACAAACACTGCATCTGCCTTCACTAAGAGCTTCAGAAGCCCTAGAAAAGTCGAAGTCCCAACTGAAATCGACGAGAACCTCTTCTTCACAATTGGTCTCGGACTCAACAAATGCCCTAAGCACTTTCGATCTAGAAGGTGCCAAGGCCCCAATGGCACACGCTTCACTGCTAGCATGAACAATGTCTCATTTGTGCTTCCAAACAACATGTCAATTCTCCATGCTTACCAGCAAAACATTCCTGGAGTTTACACTAGTGATTTTCCAGCCAACCCACCACGGAAATTTGATTACACTGGGAATGTCAGCAGCTCGCTTTGGCAACCCAGTTCTGGAACAAAGGGATACAAGTTGAAGTATGGGTCTAGAGTGCAGGTGGTGTTACAAGACACAAGTATTGTCACACCAGAGAACCATCCAATTCATCTTCATGGATATGATTTCTACATCCTTGCAGAGGGTTTTGGAAACTTCAATGCCAAGACGGATACTAAAAAGTTTAACCTTGTTGATCCACCTCTGAGGAGCACTGTGTCAGTGCCCGCAAATGGTTGGGCAGTCATCAGATTTGTAGCTGACAATCCAGGTGCTTGGGTGATGCATTGTCACTTGGATGTTCATATCAACTGGGGTCTGGCCATGGTTTTCTTGGTGGACAATGGAGTTGGCGAGCTGAAAACAATAAAGCCTCCGCCTGCAGATTTGCCTCTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

267

Amino Acids

29.53

Weight (kDa)

9.37

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 116 - 249 4.3e-43 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018953)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0341981 RchiOBHm_Chr1g0342181
rosa_laevigata RLG00000029135
rosa_multiflora Rmu_co8426429.1_g000001 Rmu_sc0002161.1_g000002
rosa_samantha Rh1AG170300 Rh1BG135100 Rh1CG155300 Rh1CG158500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 451
Acc36I ACCTGC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 225
AciI CCGC 2 cut(s) 630, 779
AclWI GGATC 1 cut(s) 593
AcoI YGGCCR 1 cut(s) 723
AcsI RAATTY 1 cut(s) 371
AcuI CTGAAG 1 cut(s) 103
AfiI CCNNNNNNNGG 3 cut(s) 419, 544, 635
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 3 cut(s) 82, 442, 562
AhlI ACTAGT 2 cut(s) 23, 341
AjnI CCWGG 2 cut(s) 328, 670
AluBI AGCT 6 cut(s) 70, 77, 117, 399, 662, 760
AluI AGCT 6 cut(s) 70, 77, 117, 399, 662, 760
Alw21I GWGCWC 1 cut(s) 617
Alw26I GTCTC 2 cut(s) 188, 277
AlwI GGATC 1 cut(s) 593
AoxI GGCC 2 cut(s) 232, 723
ApeKI GCWGC 2 cut(s) 70, 396
ApoI RAATTY 1 cut(s) 371
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
Asp700I GAANNNNTTC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 694
AsuNHI GCTAGC 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 630
BalI TGGCCA 1 cut(s) 725
BanI GGYRCC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 617
BbvI GCAGC 2 cut(s) 57, 408
BccI CCATC 1 cut(s) 510
BciT130I CCWGG 2 cut(s) 330, 672
BciVI GTATCC 2 cut(s) 425, 568
BcoDI GTCTC 2 cut(s) 188, 277
BcuI ACTAGT 2 cut(s) 23, 341
BfaI CTAG 6 cut(s) 24, 129, 219, 258, 342, 453
BfmI CTRYAG 1 cut(s) 783
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 2 cut(s) 425, 568
BglI GCCNNNNNGGC 1 cut(s) 240
BisI GCNGC 2 cut(s) 71, 397
BlsI GCNGC 2 cut(s) 72, 398
Bme1390I CCNGG 2 cut(s) 330, 672
BmgT120I GGNCC 1 cut(s) 233
BmiI GGNNCC 2 cut(s) 227, 235
BmrFI CCNGG 2 cut(s) 330, 672
BmrI ACTGGG 3 cut(s) 393, 408, 724
BmsI GCATC 2 cut(s) 107, 675
BmtI GCTAGC 1 cut(s) 261
BmuI ACTGGG 3 cut(s) 393, 408, 724
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 229, 365, 726
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 544, 635
Bse1I ACTGG 3 cut(s) 388, 414, 719
BseBI CCWGG 2 cut(s) 330, 672
BseDI CCNNGG 3 cut(s) 229, 365, 726
BseGI GGATG 3 cut(s) 502, 534, 706
BseLI CCNNNNNNNGG 3 cut(s) 419, 544, 635
BseMII CTCAG 1 cut(s) 599
BseNI ACTGG 3 cut(s) 388, 414, 719
BseRI GAGGAG 1 cut(s) 625
BseSI GKGCMC 1 cut(s) 630
BseXI GCAGC 2 cut(s) 57, 408
BsgI GTGCAG 1 cut(s) 479
BshFI GGCC 2 cut(s) 234, 725
BshNI GGYRCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 617
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 3 cut(s) 419, 544, 635
BsmAI GTCTC 2 cut(s) 188, 277
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 2 cut(s) 234, 725
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 617, 630
Bsp143I GATC 2 cut(s) 215, 598
Bsp19I CCATGG 1 cut(s) 726
BspACI CCGC 2 cut(s) 630, 779
BspANI GGCC 2 cut(s) 234, 725
BspCNI CTCAG 1 cut(s) 600
BspLI GGNNCC 2 cut(s) 227, 235
BspMAI CTGCAG 1 cut(s) 787
BspMI ACCTGC 1 cut(s) 451
BspOI GCTAGC 1 cut(s) 261
BspPI GGATC 1 cut(s) 593
BspT107I GGYRCC 1 cut(s) 225
BspTNI GGTCTC 1 cut(s) 188
BsrI ACTGG 3 cut(s) 388, 414, 719
BssECI CCNNGG 3 cut(s) 229, 365, 726
BssMI GATC 2 cut(s) 215, 598
BssT1I CCWWGG 2 cut(s) 229, 726
Bst2UI CCWGG 2 cut(s) 330, 672
Bst4CI ACNGT 2 cut(s) 46, 619
Bst6I CTCTTC 1 cut(s) 173
BstC8I GCNNGC 6 cut(s) 75, 259, 401, 630, 758, 783
BstDEI CTNAG 3 cut(s) 111, 204, 608
BstDSI CCRYGG 2 cut(s) 365, 726
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 3 cut(s) 502, 534, 706
BstKTI GATC 2 cut(s) 218, 601
BstMAI GTCTC 2 cut(s) 188, 277
BstMBI GATC 2 cut(s) 215, 598
BstMWI GCNNNNNNNGC 3 cut(s) 70, 123, 240
BstNI CCWGG 2 cut(s) 330, 672
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 2 cut(s) 328, 670
BstSFI CTRYAG 1 cut(s) 783
BstSLI GKGCMC 1 cut(s) 630
BstV1I GCAGC 2 cut(s) 57, 408
BstXI CCANNNNNNTGG 1 cut(s) 678
BsuI GTATCC 2 cut(s) 425, 568
BsuRI GGCC 2 cut(s) 234, 725
BtgI CCRYGG 2 cut(s) 365, 726
BtsCI GGATG 3 cut(s) 502, 534, 706
BtsI GCAGTG 2 cut(s) 93, 252
BtsIMutI CAGTG 5 cut(s) 93, 252, 381, 615, 630
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 6 cut(s) 75, 259, 401, 630, 758, 783
Cfr13I GGNCC 1 cut(s) 233
CviAII CATG 5 cut(s) 262, 295, 308, 518, 727
DdeI CTNAG 3 cut(s) 111, 204, 608
DpnI GATC 2 cut(s) 217, 600
DpnII GATC 2 cut(s) 215, 598
EaeI YGGCCR 1 cut(s) 723
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
EciI GGCGGA 1 cut(s) 768
Eco130I CCWWGG 2 cut(s) 229, 726
Eco31I GGTCTC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 103
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 328, 670
EcoT14I CCWWGG 2 cut(s) 229, 726
EcoT22I ATGCAT 1 cut(s) 690
ErhI CCWWGG 2 cut(s) 229, 726
FaeI CATG 5 cut(s) 265, 298, 311, 521, 730
FaiI YATR 9 cut(s) 59, 263, 296, 309, 447, 519, 525, 708, 728
FaqI GGGAC 1 cut(s) 128
FatI CATG 5 cut(s) 261, 294, 307, 517, 726
FauI CCCGC 1 cut(s) 637
Fnu4HI GCNGC 2 cut(s) 71, 397
FokI GGATG 3 cut(s) 489, 521, 713
Fsp4HI GCNGC 2 cut(s) 71, 397
FspBI CTAG 6 cut(s) 24, 129, 219, 258, 342, 453
GluI GCNGC 2 cut(s) 71, 397
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 2 cut(s) 234, 725
Hin1II CATG 5 cut(s) 265, 298, 311, 521, 730
HinfI GANTC 1 cut(s) 189
HphI GGTGA 1 cut(s) 694
Hpy166II GTNNAC 2 cut(s) 337, 742
Hpy188I TCNGA 4 cut(s) 122, 188, 609, 653
Hpy188III TCNNGA 3 cut(s) 219, 420, 453
Hpy8I GTNNAC 2 cut(s) 337, 742
Hpy99I CGWCG 2 cut(s) 11, 161
HpyAV CCTTC 2 cut(s) 115, 216
HpyCH4III ACNGT 2 cut(s) 46, 619
HpyCH4V TGCA 7 cut(s) 15, 30, 98, 460, 542, 688, 785
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 123, 240
HpyF3I CTNAG 3 cut(s) 111, 204, 608
Hsp92II CATG 5 cut(s) 265, 298, 311, 521, 730
Kzo9I GATC 2 cut(s) 215, 598
LmnI GCTCC 1 cut(s) 612
Lsp1109I GCAGC 2 cut(s) 57, 408
LweI GCATC 2 cut(s) 107, 675
MaeI CTAG 6 cut(s) 24, 129, 219, 258, 342, 453
MaeIII GTNAC 3 cut(s) 468, 487, 692
MalI GATC 2 cut(s) 217, 600
MboI GATC 2 cut(s) 215, 598
MboII GAAGA 3 cut(s) 160, 163, 506
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 2 cut(s) 617, 630
MlsI TGGCCA 1 cut(s) 725
MluCI AATT 5 cut(s) 48, 177, 300, 371, 507
MluNI TGGCCA 1 cut(s) 725
MlyI GAGTC 1 cut(s) 183
MnlI CCTC 6 cut(s) 176, 538, 603, 615, 786, 804
Mox20I TGGCCA 1 cut(s) 725
Mph1103I ATGCAT 1 cut(s) 690
MroXI GAANNNNTTC 1 cut(s) 167
MscI TGGCCA 1 cut(s) 725
MseI TTAA 3 cut(s) 51, 588, 802
MslI CAYNNNNRTG 3 cut(s) 516, 522, 699
Msp20I TGGCCA 1 cut(s) 725
MspR9I CCNGG 2 cut(s) 330, 672
MunI CAATTG 1 cut(s) 177
MvaI CCWGG 2 cut(s) 330, 672
MwoI GCNNNNNNNGC 3 cut(s) 70, 123, 240
NcoI CCATGG 1 cut(s) 726
NdeII GATC 2 cut(s) 215, 598
NheI GCTAGC 1 cut(s) 257
NlaIII CATG 5 cut(s) 265, 298, 311, 521, 730
NlaIV GGNNCC 2 cut(s) 227, 235
NmuCI GTSAC 2 cut(s) 487, 692
NsiI ATGCAT 1 cut(s) 690
NspI RCATGY 1 cut(s) 298
PaqCI CACCTGC 1 cut(s) 451
PciI ACATGT 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 328
PkrI GCNGC 2 cut(s) 72, 398
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PscI ACATGT 1 cut(s) 294
Psp6I CCWGG 2 cut(s) 328, 670
PspGI CCWGG 2 cut(s) 328, 670
PspN4I GGNNCC 2 cut(s) 227, 235
PspPI GGNCC 1 cut(s) 233
PstI CTGCAG 1 cut(s) 787
RseI CAYNNNNRTG 3 cut(s) 516, 522, 699
SaqAI TTAA 3 cut(s) 51, 588, 802
SatI GCNGC 2 cut(s) 71, 397
Sau3AI GATC 2 cut(s) 215, 598
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 2 cut(s) 330, 672
SduI GDGCHC 2 cut(s) 617, 630
SfaNI GCATC 2 cut(s) 107, 675
SfcI CTRYAG 1 cut(s) 783
SmiMI CAYNNNNRTG 3 cut(s) 516, 522, 699
SpeI ACTAGT 2 cut(s) 23, 341
Sse9I AATT 5 cut(s) 48, 177, 300, 371, 507
SsiI CCGC 2 cut(s) 630, 779
SspMI CTAG 6 cut(s) 24, 129, 219, 258, 342, 453
StyD4I CCNGG 2 cut(s) 328, 670
StyI CCWWGG 2 cut(s) 229, 726
TaaI ACNGT 2 cut(s) 46, 619
TaqI TCGA 3 cut(s) 138, 156, 214
TasI AATT 5 cut(s) 48, 177, 300, 371, 507
Tru1I TTAA 3 cut(s) 51, 588, 802
Tru9I TTAA 3 cut(s) 51, 588, 802
TscAI CASTG 5 cut(s) 100, 259, 388, 622, 630
TseFI GTSAC 2 cut(s) 487, 692
TseI GCWGC 2 cut(s) 70, 396
Tsp45I GTSAC 2 cut(s) 487, 692
TspDTI ATGAA 4 cut(s) 278, 500, 506, 695
TspGWI ACGGA 2 cut(s) 382, 587
TspRI CASTG 5 cut(s) 100, 259, 388, 622, 630
XagI CCTNNNNNAGG 1 cut(s) 542
XapI RAATTY 1 cut(s) 371
XbaI TCTAGA 2 cut(s) 218, 452
XceI RCATGY 1 cut(s) 298
XcmI CCANNNNNNNNNTGG 2 cut(s) 236, 733
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 6 cut(s) 24, 129, 219, 258, 342, 453
Zsp2I ATGCAT 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.