RchiOBHm_Chr1g0342181

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018953
Physical Location & Seq
Reverse (-)
34137842 .. 34138475
634 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56883

Sequence Viewer

Length: 546 bp
ATGAACAATGTCTCATTTGTGCTTCCAAACAACATGTCAATTCTCCAAGCTTACCAGCAAAACATTCCTGGAGTTTACACTAGTGATTTTCCAGCCAACCCGCCACGGAAGTTTGATTACAATGGGAATGTGAGCCGCTCGCTTTGGCAACCCAGTTCTGGAACAAAGGCATACAAGTTGAAGTACGGGTCTAGGGTGCAGGTGGTGTTACAAGACAGAAGTATTGTCACACCCGAGAACCATCCCATTCATCTCCACGGTTATGATTTCTACATCCTTGCAGAGGGTTTTGGAAATTTCAACCCCAAGACTGATACTAAAATGTTTAATCTTGTTGATCCACCTCTAAGGAACACGGTCGCGGTGCCCGCAAATGGTTGGGCAGTCATCAGATTTGTAGCTGACAATCCAGGTGCTTGGATGATGCATTGTCACTTGGATGTTCATATCAACTGGGGTTTGGCCATGGTTTTCTTGGTGGACAATGGAGTTGGGGAGCTGCAATCAATAGAGCCACCGCCAGCGGATTATCCTCTGTGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

181

Amino Acids

20.28

Weight (kDa)

6.27

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 29 - 162 4.9e-44 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018953)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0341981 RchiOBHm_Chr1g0342181
rosa_laevigata RLG00000029135
rosa_multiflora Rmu_co8426429.1_g000001 Rmu_sc0002161.1_g000002
rosa_samantha Rh1AG170300 Rh1BG135100 Rh1CG155300 Rh1CG158500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 364
AccBSI CCGCTC 1 cut(s) 138
AccII CGCG 1 cut(s) 362
AciI CCGC 6 cut(s) 101, 136, 362, 369, 518, 524
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 1 cut(s) 295
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 158, 283, 374
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 2 cut(s) 181, 301
AhlI ACTAGT 1 cut(s) 80
AjnI CCWGG 2 cut(s) 67, 409
AluBI AGCT 3 cut(s) 50, 401, 499
AluI AGCT 3 cut(s) 50, 401, 499
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 1 cut(s) 499
ApoI RAATTY 1 cut(s) 295
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
AvaI CYCGRG 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 369
BalI TGGCCA 1 cut(s) 464
BanI GGYRCC 1 cut(s) 364
BarI GAAGNNNNNNTAC 2 cut(s) 101, 133
BbvI GCAGC 1 cut(s) 486
BccI CCATC 1 cut(s) 249
BciT130I CCWGG 2 cut(s) 69, 411
BcoDI GTCTC 1 cut(s) 16
BcuI ACTAGT 1 cut(s) 80
BfaI CTAG 2 cut(s) 81, 192
BfuAI ACCTGC 1 cut(s) 190
BisI GCNGC 2 cut(s) 136, 500
BlsI GCNGC 2 cut(s) 137, 501
Bme1390I CCNGG 2 cut(s) 69, 411
BmeT110I CYCGRG 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 2 cut(s) 69, 411
BmrI ACTGGG 2 cut(s) 147, 463
BmsI GCATC 1 cut(s) 414
BmuI ACTGGG 2 cut(s) 147, 463
BpmI CTGGAG 1 cut(s) 90
BsaJI CCNNGG 3 cut(s) 104, 256, 465
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 283, 374
Bse1I ACTGG 2 cut(s) 153, 458
BseBI CCWGG 2 cut(s) 69, 411
BseDI CCNNGG 3 cut(s) 104, 256, 465
BseGI GGATG 4 cut(s) 241, 273, 426, 445
BseLI CCNNNNNNNGG 3 cut(s) 158, 283, 374
BseNI ACTGG 2 cut(s) 153, 458
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 1 cut(s) 486
BsgI GTGCAG 1 cut(s) 218
Bsh1236I CGCG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 1 cut(s) 464
BshNI GGYRCC 1 cut(s) 364
BsiEI CGRYCG 1 cut(s) 360
BsiHKCI CYCGRG 1 cut(s) 233
BslI CCNNNNNNNGG 3 cut(s) 158, 283, 374
BsmAI GTCTC 1 cut(s) 16
BsnI GGCC 1 cut(s) 464
BsoBI CYCGRG 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 1 cut(s) 337
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 6 cut(s) 101, 136, 362, 369, 518, 524
BspANI GGCC 1 cut(s) 464
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 1 cut(s) 366
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 364
BsrBI CCGCTC 1 cut(s) 138
BsrI ACTGG 2 cut(s) 153, 458
BssECI CCNNGG 3 cut(s) 104, 256, 465
BssMI GATC 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 69, 411
Bst4CI ACNGT 2 cut(s) 260, 358
BstC8I GCNNGC 3 cut(s) 140, 369, 522
BstDEI CTNAG 1 cut(s) 347
BstDSI CCRYGG 3 cut(s) 104, 256, 465
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 4 cut(s) 241, 273, 426, 445
BstFNI CGCG 1 cut(s) 362
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 1 cut(s) 337
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 2 cut(s) 69, 411
BstNSI RCATGY 1 cut(s) 37
BstSCI CCNGG 2 cut(s) 67, 409
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 1 cut(s) 486
BstXI CCANNNNNNTGG 1 cut(s) 417
BsuRI GGCC 1 cut(s) 464
BtgI CCRYGG 3 cut(s) 104, 256, 465
BtsCI GGATG 4 cut(s) 241, 273, 426, 445
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 3 cut(s) 140, 369, 522
Csp6I GTAC 1 cut(s) 184
CviAII CATG 2 cut(s) 34, 466
CviJI RGCY 7 cut(s) 50, 95, 135, 401, 464, 499, 514
CviKI_1 RGCY 7 cut(s) 50, 95, 135, 401, 464, 499, 514
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 1 cut(s) 347
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
EaeI YGGCCR 1 cut(s) 462
Eco130I CCWWGG 1 cut(s) 465
Eco88I CYCGRG 1 cut(s) 233
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 67, 409
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 2 cut(s) 37, 469
FaiI YATR 5 cut(s) 35, 172, 264, 447, 467
FatI CATG 2 cut(s) 33, 465
FauI CCCGC 2 cut(s) 108, 376
Fnu4HI GCNGC 2 cut(s) 136, 500
FokI GGATG 4 cut(s) 228, 260, 433, 452
Fsp4HI GCNGC 2 cut(s) 136, 500
FspBI CTAG 2 cut(s) 81, 192
GluI GCNGC 2 cut(s) 136, 500
GsuI CTGGAG 1 cut(s) 90
HaeIII GGCC 1 cut(s) 464
Hin1II CATG 2 cut(s) 37, 469
HindIII AAGCTT 1 cut(s) 48
Hpy166II GTNNAC 2 cut(s) 76, 481
Hpy188I TCNGA 1 cut(s) 392
Hpy188III TCNNGA 1 cut(s) 159
Hpy8I GTNNAC 2 cut(s) 76, 481
HpyCH4III ACNGT 2 cut(s) 260, 358
HpyCH4V TGCA 4 cut(s) 199, 281, 427, 502
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 1 cut(s) 347
Hsp92II CATG 2 cut(s) 37, 469
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 1 cut(s) 486
LweI GCATC 1 cut(s) 414
MaeI CTAG 2 cut(s) 81, 192
MaeIII GTNAC 3 cut(s) 207, 226, 431
MalI GATC 1 cut(s) 339
MbiI CCGCTC 1 cut(s) 138
MboI GATC 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 369
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 2 cut(s) 39, 295
MluNI TGGCCA 1 cut(s) 464
MnlI CCTC 3 cut(s) 277, 354, 543
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 1 cut(s) 429
MscI TGGCCA 1 cut(s) 464
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 2 cut(s) 261, 438
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 524
MspR9I CCNGG 2 cut(s) 69, 411
MvaI CCWGG 2 cut(s) 69, 411
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 1 cut(s) 368
NcoI CCATGG 1 cut(s) 465
NdeII GATC 1 cut(s) 337
NlaIII CATG 2 cut(s) 37, 469
NlaIV GGNNCC 1 cut(s) 366
NmuCI GTSAC 2 cut(s) 226, 431
NsiI ATGCAT 1 cut(s) 429
NspI RCATGY 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 190
PciI ACATGT 1 cut(s) 33
PfoI TCCNGGA 1 cut(s) 67
PkrI GCNGC 2 cut(s) 137, 501
PscI ACATGT 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 67, 409
PspGI CCWGG 2 cut(s) 67, 409
PspN4I GGNNCC 1 cut(s) 366
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
RseI CAYNNNNRTG 2 cut(s) 261, 438
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 2 cut(s) 136, 500
Sau3AI GATC 1 cut(s) 337
ScrFI CCNGG 2 cut(s) 69, 411
SduI GDGCHC 1 cut(s) 369
SetI ASST 6 cut(s) 52, 204, 346, 403, 415, 501
SfaNI GCATC 1 cut(s) 414
SmiMI CAYNNNNRTG 2 cut(s) 261, 438
SpeI ACTAGT 1 cut(s) 80
Sse9I AATT 2 cut(s) 39, 295
SsiI CCGC 6 cut(s) 101, 136, 362, 369, 518, 524
SspMI CTAG 2 cut(s) 81, 192
StyD4I CCNGG 2 cut(s) 67, 409
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 2 cut(s) 260, 358
TasI AATT 2 cut(s) 39, 295
TauI GCSGC 1 cut(s) 138
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseFI GTSAC 2 cut(s) 226, 431
TseI GCWGC 1 cut(s) 499
Tsp45I GTSAC 2 cut(s) 226, 431
TspDTI ATGAA 3 cut(s) 17, 239, 434
TspGWI ACGGA 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 281
XapI RAATTY 1 cut(s) 295
XceI RCATGY 1 cut(s) 37
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XspI CTAG 2 cut(s) 81, 192
Zsp2I ATGCAT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.