Rh1CG155300

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0018953
Physical Location & Seq
Forward (+)
34279026 .. 34279850
825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG155300.1

Sequence Viewer

Length: 747 bp
ATGCCGAAGCTGCCAGCTTTCAATGACACAAACACTGCATCTGCCTTCACTAAGAGCTTCAGAAGCCCTAGAAAAGTCGAAGTCCCAACTGAAATCGACGAGAACCTCTTCTTCACAATCGGTCTCGGACTCAACAAATGCCCCAAGCACTTTCTATCTAGAAGATGCCAAGGCCCCAATGGCACGCGCTTCACTGCTAGCATGAACAATGTCTCATTTGTGCTTCCAAACAACATGTCAATTCTCCAAGCTTACCAGCAAAACATTCCTGGAGTTTACACTAGTGATTTTCCAGCCAACCCGCCACGGAAGTTTGATTACACTGGGAATGTGAGCCGCTCGCTTTGGCAACCCAGTTCTGGAACAAAGGCATACAAGTTGAAGTACGGGTCTAGGGTGCAGGTGGTGTTACAAGACACAAGTATTGTCACACCCGAGAACCATCCCATTCATCTCCACGGTTATGATTTCTACATCCTTGCAGAGGGTTTTGGAAATTTCAACCCCAAGACTGATACTAAAATGTTTAATCTTGTTGATCCACCTCTAAGGAACACGGTCGCGGTGCCCGTAAATGGTTGGGCAGTCATCAGATTTGTAGCTGACAATCCAGGTGCTTGGATGATGCATTGTCACTTGGATGTTCATATCAACTGGGGTTTGGCCATGGTTTTCTTGGTGGACAATGGAGTTGGGGAGCTGCAATCAATAGAGCCACCGCCAGCGGATTATCCTCTGTGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

248

Amino Acids

27.71

Weight (kDa)

8.32

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 96 - 229 3.3e-44 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018953)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0341981 RchiOBHm_Chr1g0342181
rosa_laevigata RLG00000029135
rosa_multiflora Rmu_co8426429.1_g000001 Rmu_sc0002161.1_g000002
rosa_samantha Rh1AG170300 Rh1BG135100 Rh1CG155300 Rh1CG158500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 565
AccBSI CCGCTC 1 cut(s) 339
AccII CGCG 2 cut(s) 187, 563
AciI CCGC 5 cut(s) 302, 337, 563, 719, 725
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 1 cut(s) 496
AcuI CTGAAG 1 cut(s) 43
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 3 cut(s) 359, 484, 575
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 3 cut(s) 22, 382, 502
AhlI ACTAGT 1 cut(s) 281
AjnI CCWGG 2 cut(s) 268, 610
AluBI AGCT 6 cut(s) 10, 17, 57, 251, 602, 700
AluI AGCT 6 cut(s) 10, 17, 57, 251, 602, 700
Alw26I GTCTC 2 cut(s) 128, 217
AlwI GGATC 1 cut(s) 533
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 2 cut(s) 172, 663
ApeKI GCWGC 2 cut(s) 10, 700
ApoI RAATTY 1 cut(s) 496
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 1 cut(s) 173
AsuNHI GCTAGC 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 570
BalI TGGCCA 1 cut(s) 665
BanI GGYRCC 1 cut(s) 565
BarI GAAGNNNNNNTAC 2 cut(s) 302, 334
BbvI GCAGC 1 cut(s) 687
BccI CCATC 1 cut(s) 450
BciT130I CCWGG 2 cut(s) 270, 612
BcoDI GTCTC 2 cut(s) 128, 217
BcuI ACTAGT 1 cut(s) 281
BfaI CTAG 5 cut(s) 69, 159, 198, 282, 393
BfuAI ACCTGC 1 cut(s) 391
BglI GCCNNNNNGGC 1 cut(s) 180
BisI GCNGC 3 cut(s) 11, 337, 701
BlsI GCNGC 3 cut(s) 12, 338, 702
Bme1390I CCNGG 2 cut(s) 270, 612
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 175, 567
BmrFI CCNGG 2 cut(s) 270, 612
BmrI ACTGGG 3 cut(s) 333, 348, 664
BmsI GCATC 3 cut(s) 47, 155, 615
BmtI GCTAGC 1 cut(s) 201
BmuI ACTGGG 3 cut(s) 333, 348, 664
BpmI CTGGAG 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 4 cut(s) 169, 305, 457, 666
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 484, 575
Bse1I ACTGG 3 cut(s) 328, 354, 659
BseBI CCWGG 2 cut(s) 270, 612
BseDI CCNNGG 4 cut(s) 169, 305, 457, 666
BseGI GGATG 4 cut(s) 442, 474, 627, 646
BseLI CCNNNNNNNGG 3 cut(s) 359, 484, 575
BseNI ACTGG 3 cut(s) 328, 354, 659
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 1 cut(s) 687
BsgI GTGCAG 1 cut(s) 419
Bsh1236I CGCG 2 cut(s) 187, 563
Bsh1285I CGRYCG 1 cut(s) 561
BshFI GGCC 2 cut(s) 174, 665
BshNI GGYRCC 1 cut(s) 565
BsiEI CGRYCG 1 cut(s) 561
BsiHKCI CYCGRG 1 cut(s) 434
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 3 cut(s) 359, 484, 575
BsmAI GTCTC 2 cut(s) 128, 217
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 174, 665
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 1 cut(s) 570
Bsp143I GATC 1 cut(s) 538
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 5 cut(s) 302, 337, 563, 719, 725
BspANI GGCC 2 cut(s) 174, 665
BspFNI CGCG 2 cut(s) 187, 563
BspLI GGNNCC 2 cut(s) 175, 567
BspMI ACCTGC 1 cut(s) 391
BspOI GCTAGC 1 cut(s) 201
BspPI GGATC 1 cut(s) 533
BspT107I GGYRCC 1 cut(s) 565
BspTNI GGTCTC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 339
BsrI ACTGG 3 cut(s) 328, 354, 659
BssECI CCNNGG 4 cut(s) 169, 305, 457, 666
BssMI GATC 1 cut(s) 538
BssT1I CCWWGG 2 cut(s) 169, 666
Bst2UI CCWGG 2 cut(s) 270, 612
Bst4CI ACNGT 2 cut(s) 461, 559
Bst6I CTCTTC 1 cut(s) 113
BstC8I GCNNGC 5 cut(s) 15, 185, 199, 341, 723
BstDEI CTNAG 2 cut(s) 51, 548
BstDSI CCRYGG 3 cut(s) 305, 457, 666
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 4 cut(s) 442, 474, 627, 646
BstFNI CGCG 2 cut(s) 187, 563
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 1 cut(s) 541
BstMAI GTCTC 2 cut(s) 128, 217
BstMBI GATC 1 cut(s) 538
BstMCI CGRYCG 1 cut(s) 561
BstMWI GCNNNNNNNGC 3 cut(s) 10, 63, 180
BstNI CCWGG 2 cut(s) 270, 612
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 268, 610
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 2 cut(s) 187, 563
BstV1I GCAGC 1 cut(s) 687
BstXI CCANNNNNNTGG 1 cut(s) 618
BsuRI GGCC 2 cut(s) 174, 665
BtgI CCRYGG 3 cut(s) 305, 457, 666
BtsCI GGATG 4 cut(s) 442, 474, 627, 646
BtsI GCAGTG 2 cut(s) 33, 192
BtsIMutI CAGTG 3 cut(s) 33, 192, 321
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 5 cut(s) 15, 185, 199, 341, 723
CfoI GCGC 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 173
Csp6I GTAC 1 cut(s) 385
CviAII CATG 3 cut(s) 202, 235, 667
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 51, 548
DpnI GATC 1 cut(s) 540
DpnII GATC 1 cut(s) 538
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 169, 666
Eco31I GGTCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 434
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 173
EcoRII CCWGG 2 cut(s) 268, 610
EcoT14I CCWWGG 2 cut(s) 169, 666
EcoT22I ATGCAT 1 cut(s) 630
ErhI CCWWGG 2 cut(s) 169, 666
FaeI CATG 3 cut(s) 205, 238, 670
FaiI YATR 6 cut(s) 203, 236, 373, 465, 648, 668
FaqI GGGAC 1 cut(s) 68
FatI CATG 3 cut(s) 201, 234, 666
FauI CCCGC 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 11, 337, 701
FokI GGATG 4 cut(s) 429, 461, 634, 653
Fsp4HI GCNGC 3 cut(s) 11, 337, 701
FspBI CTAG 5 cut(s) 69, 159, 198, 282, 393
GlaI GCGC 1 cut(s) 188
GluI GCNGC 3 cut(s) 11, 337, 701
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 2 cut(s) 174, 665
HhaI GCGC 1 cut(s) 189
Hin1II CATG 3 cut(s) 205, 238, 670
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 1 cut(s) 129
Hpy166II GTNNAC 2 cut(s) 277, 682
Hpy188I TCNGA 3 cut(s) 62, 128, 593
Hpy188III TCNNGA 2 cut(s) 159, 360
Hpy8I GTNNAC 2 cut(s) 277, 682
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 2 cut(s) 461, 559
HpyCH4V TGCA 5 cut(s) 38, 400, 482, 628, 703
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 63, 180
HpyF3I CTNAG 2 cut(s) 51, 548
Hsp92II CATG 3 cut(s) 205, 238, 670
HspAI GCGC 1 cut(s) 187
Kzo9I GATC 1 cut(s) 538
LmnI GCTCC 1 cut(s) 697
Lsp1109I GCAGC 1 cut(s) 687
LweI GCATC 3 cut(s) 47, 155, 615
MaeI CTAG 5 cut(s) 69, 159, 198, 282, 393
MaeIII GTNAC 3 cut(s) 408, 427, 632
MalI GATC 1 cut(s) 540
MbiI CCGCTC 1 cut(s) 339
MboI GATC 1 cut(s) 538
MboII GAAGA 3 cut(s) 100, 103, 174
MhlI GDGCHC 1 cut(s) 570
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 2 cut(s) 240, 496
MluNI TGGCCA 1 cut(s) 665
MlyI GAGTC 1 cut(s) 123
MnlI CCTC 4 cut(s) 116, 478, 555, 744
Mox20I TGGCCA 1 cut(s) 665
Mph1103I ATGCAT 1 cut(s) 630
MroXI GAANNNNTTC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 665
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 2 cut(s) 462, 639
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 1 cut(s) 725
MspR9I CCNGG 2 cut(s) 270, 612
MvaI CCWGG 2 cut(s) 270, 612
MvnI CGCG 2 cut(s) 187, 563
MwoI GCNNNNNNNGC 3 cut(s) 10, 63, 180
NcoI CCATGG 1 cut(s) 666
NdeII GATC 1 cut(s) 538
NheI GCTAGC 1 cut(s) 197
NlaIII CATG 3 cut(s) 205, 238, 670
NlaIV GGNNCC 2 cut(s) 175, 567
NmuCI GTSAC 2 cut(s) 427, 632
NsiI ATGCAT 1 cut(s) 630
NspI RCATGY 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 391
PciI ACATGT 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 567
PdmI GAANNNNTTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 268
PkrI GCNGC 3 cut(s) 12, 338, 702
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
PscI ACATGT 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 268, 610
PspGI CCWGG 2 cut(s) 268, 610
PspN4I GGNNCC 2 cut(s) 175, 567
PspPI GGNCC 1 cut(s) 173
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 2 cut(s) 462, 639
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 3 cut(s) 11, 337, 701
Sau3AI GATC 1 cut(s) 538
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 2 cut(s) 270, 612
SduI GDGCHC 1 cut(s) 570
SfaNI GCATC 3 cut(s) 47, 155, 615
SmiMI CAYNNNNRTG 2 cut(s) 462, 639
SpeI ACTAGT 1 cut(s) 281
Sse9I AATT 2 cut(s) 240, 496
SsiI CCGC 5 cut(s) 302, 337, 563, 719, 725
SspMI CTAG 5 cut(s) 69, 159, 198, 282, 393
StyD4I CCNGG 2 cut(s) 268, 610
StyI CCWWGG 2 cut(s) 169, 666
TaaI ACNGT 2 cut(s) 461, 559
TaqI TCGA 2 cut(s) 78, 96
TaqII GACCGA 1 cut(s) 110
TasI AATT 2 cut(s) 240, 496
TauI GCSGC 1 cut(s) 339
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 3 cut(s) 40, 199, 328
TseFI GTSAC 2 cut(s) 427, 632
TseI GCWGC 2 cut(s) 10, 700
Tsp45I GTSAC 2 cut(s) 427, 632
TspDTI ATGAA 3 cut(s) 218, 440, 635
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 3 cut(s) 40, 199, 328
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 496
XbaI TCTAGA 1 cut(s) 158
XceI RCATGY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 673
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 5 cut(s) 69, 159, 198, 282, 393
Zsp2I ATGCAT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.