RLG00000029135

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018953
Physical Location & Seq
Reverse (-)
33565058 .. 33565682
625 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029135

Sequence Viewer

Length: 510 bp
ATGTCAATTCTCCAAGCTTACCAGCAAAACATTCCTGGAGTTTACACTAGTGATTTTCCAGCCAACCTGCCACAGAAATTTGATTACACTGGGAATGTCAGCCTATCGCTTTGGCAACCCAGTTCTGGAACAAAGGGATACAAGTTGAAGTACGGGTCTAGAGTGCAGGTGGTGTTACAAGACACAAGTATTGTCACACCCGACGACCATCCTATTCATCTCCACGGATATGATTTCTACATCCTTGCAGAGGGTTTTGGAAATTTCAATGCCAAGACTGATACTAAAAAGCTTAACCTTGTTGATCCACCTCTGAGGAACACAGTGTTGGTGCCCTCAAATGGTTGGGCAGTCATCAGATTTGTAGCTGACAATCCAGGTGCTTGGCTGATGCATTGTCACTTGGATGTACATATCAACTGGGCTTTGGCTATGGTTTTCTTGGTGGACAATGGAGTTGGGGAGCTGCAGGCAATAAAGCCTCCGCCTGCAGATTTGCCTATGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

170

Amino Acids

18.75

Weight (kDa)

6.01

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 18 - 151 4.5e-42 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018953)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0341981 RchiOBHm_Chr1g0342181
rosa_laevigata RLG00000029135
rosa_multiflora Rmu_co8426429.1_g000001 Rmu_sc0002161.1_g000002
rosa_samantha Rh1AG170300 Rh1BG135100 Rh1CG155300 Rh1CG158500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 2 cut(s) 75, 157
AccB1I GGYRCC 1 cut(s) 331
AciI CCGC 1 cut(s) 485
AclWI GGATC 1 cut(s) 299
AcsI RAATTY 2 cut(s) 77, 262
AfaI GTAC 2 cut(s) 152, 411
AfiI CCNNNNNNNGG 3 cut(s) 125, 250, 341
AgsI TTSAA 2 cut(s) 148, 268
AhlI ACTAGT 1 cut(s) 47
AjnI CCWGG 2 cut(s) 34, 376
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 4 cut(s) 17, 292, 368, 466
AluI AGCT 4 cut(s) 17, 292, 368, 466
AlwI GGATC 1 cut(s) 299
ApeKI GCWGC 1 cut(s) 466
ApoI RAATTY 2 cut(s) 77, 262
BaeGI GKGCMC 1 cut(s) 336
BanI GGYRCC 1 cut(s) 331
BbvI GCAGC 1 cut(s) 453
BccI CCATC 1 cut(s) 216
BciT130I CCWGG 2 cut(s) 36, 378
BciVI GTATCC 1 cut(s) 131
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 2 cut(s) 48, 159
BfmI CTRYAG 2 cut(s) 467, 489
BfuAI ACCTGC 2 cut(s) 75, 157
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 1 cut(s) 467
BlsI GCNGC 1 cut(s) 468
Bme1390I CCNGG 2 cut(s) 36, 378
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 2 cut(s) 36, 378
BmrI ACTGGG 3 cut(s) 99, 114, 430
BmsI GCATC 1 cut(s) 381
BmuI ACTGGG 3 cut(s) 99, 114, 430
BpmI CTGGAG 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 250, 341
Bse1I ACTGG 3 cut(s) 94, 120, 425
BseBI CCWGG 2 cut(s) 36, 378
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 3 cut(s) 208, 240, 412
BseLI CCNNNNNNNGG 3 cut(s) 125, 250, 341
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 3 cut(s) 94, 120, 425
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 1 cut(s) 453
BsgI GTGCAG 1 cut(s) 185
BshNI GGYRCC 1 cut(s) 331
BslI CCNNNNNNNGG 3 cut(s) 125, 250, 341
Bsp1286I GDGCHC 1 cut(s) 336
Bsp1407I TGTACA 1 cut(s) 409
Bsp143I GATC 1 cut(s) 304
BspACI CCGC 1 cut(s) 485
BspCNI CTCAG 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 333
BspMAI CTGCAG 2 cut(s) 471, 493
BspMI ACCTGC 2 cut(s) 75, 157
BspPI GGATC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 331
BsrGI TGTACA 1 cut(s) 409
BsrI ACTGG 3 cut(s) 94, 120, 425
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 1 cut(s) 304
Bst2UI CCWGG 2 cut(s) 36, 378
Bst4CI ACNGT 1 cut(s) 325
BstAUI TGTACA 1 cut(s) 409
BstC8I GCNNGC 2 cut(s) 471, 489
BstDEI CTNAG 1 cut(s) 314
BstDSI CCRYGG 1 cut(s) 223
BstENI CCTNNNNNAGG 1 cut(s) 248
BstF5I GGATG 3 cut(s) 208, 240, 412
BstKTI GATC 1 cut(s) 307
BstMBI GATC 1 cut(s) 304
BstNI CCWGG 2 cut(s) 36, 378
BstSCI CCNGG 2 cut(s) 34, 376
BstSFI CTRYAG 2 cut(s) 467, 489
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 453
BstXI CCANNNNNNTGG 1 cut(s) 384
BsuI GTATCC 1 cut(s) 131
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 3 cut(s) 208, 240, 412
BtsIMutI CAGTG 2 cut(s) 87, 330
BveI ACCTGC 2 cut(s) 75, 157
Cac8I GCNNGC 2 cut(s) 471, 489
Csp6I GTAC 2 cut(s) 151, 410
CviQI GTAC 2 cut(s) 151, 410
DdeI CTNAG 1 cut(s) 314
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
EciI GGCGGA 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 34, 376
EcoT22I ATGCAT 1 cut(s) 396
FaiI YATR 4 cut(s) 231, 414, 434, 503
Fnu4HI GCNGC 1 cut(s) 467
FokI GGATG 3 cut(s) 195, 227, 419
Fsp4HI GCNGC 1 cut(s) 467
FspBI CTAG 2 cut(s) 48, 159
GluI GCNGC 1 cut(s) 467
GsuI CTGGAG 1 cut(s) 57
HindIII AAGCTT 2 cut(s) 15, 290
Hpy166II GTNNAC 2 cut(s) 43, 448
Hpy188I TCNGA 2 cut(s) 315, 359
Hpy188III TCNNGA 2 cut(s) 126, 159
Hpy8I GTNNAC 2 cut(s) 43, 448
Hpy99I CGWCG 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4V TGCA 5 cut(s) 166, 248, 394, 469, 491
HpyF3I CTNAG 1 cut(s) 314
Kzo9I GATC 1 cut(s) 304
LmnI GCTCC 1 cut(s) 463
Lsp1109I GCAGC 1 cut(s) 453
LweI GCATC 1 cut(s) 381
MaeI CTAG 2 cut(s) 48, 159
MaeIII GTNAC 3 cut(s) 174, 193, 398
MalI GATC 1 cut(s) 306
MboI GATC 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 3 cut(s) 6, 77, 262
MnlI CCTC 5 cut(s) 244, 309, 321, 346, 492
Mph1103I ATGCAT 1 cut(s) 396
MseI TTAA 2 cut(s) 294, 508
MslI CAYNNNNRTG 2 cut(s) 228, 405
MspR9I CCNGG 2 cut(s) 36, 378
MvaI CCWGG 2 cut(s) 36, 378
NdeII GATC 1 cut(s) 304
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 2 cut(s) 193, 398
NsiI ATGCAT 1 cut(s) 396
PaqCI CACCTGC 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 34
PkrI GCNGC 1 cut(s) 468
Psp6I CCWGG 2 cut(s) 34, 376
PspGI CCWGG 2 cut(s) 34, 376
PspN4I GGNNCC 1 cut(s) 333
PstI CTGCAG 2 cut(s) 471, 493
RsaI GTAC 2 cut(s) 152, 411
RsaNI GTAC 2 cut(s) 151, 410
RseI CAYNNNNRTG 2 cut(s) 228, 405
SaqAI TTAA 2 cut(s) 294, 508
SatI GCNGC 1 cut(s) 467
Sau3AI GATC 1 cut(s) 304
ScrFI CCNGG 2 cut(s) 36, 378
SduI GDGCHC 1 cut(s) 336
SetI ASST 9 cut(s) 19, 69, 171, 294, 300, 313, 370, 382, 468
SfaNI GCATC 1 cut(s) 381
SfcI CTRYAG 2 cut(s) 467, 489
SmiMI CAYNNNNRTG 2 cut(s) 228, 405
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 3 cut(s) 6, 77, 262
SsiI CCGC 1 cut(s) 485
SspMI CTAG 2 cut(s) 48, 159
StyD4I CCNGG 2 cut(s) 34, 376
TaaI ACNGT 1 cut(s) 325
TasI AATT 3 cut(s) 6, 77, 262
TatI WGTACW 1 cut(s) 409
Tru1I TTAA 2 cut(s) 294, 508
Tru9I TTAA 2 cut(s) 294, 508
TscAI CASTG 2 cut(s) 94, 330
TseFI GTSAC 2 cut(s) 193, 398
TseI GCWGC 1 cut(s) 466
Tsp45I GTSAC 2 cut(s) 193, 398
TspDTI ATGAA 1 cut(s) 206
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 2 cut(s) 94, 330
XagI CCTNNNNNAGG 1 cut(s) 248
XapI RAATTY 2 cut(s) 77, 262
XbaI TCTAGA 1 cut(s) 158
XspI CTAG 2 cut(s) 48, 159
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.