Rh1AG170300

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0018953
Physical Location & Seq
Forward (+)
32597390 .. 32598250
861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG170300.1

Sequence Viewer

Length: 552 bp
ATGCCAAAGCTGCCAGCTTTCAATGACACAAGCACTGCATCTGCCTTCACTAAGAGCTTCAGAAGCCCTAGAAAAGTCGAAGTCCCAACTGAAATCGACGAGAACCTCTTCTTCACAATCGGTCTCGGACTCAACAAATGCCCTAAGCACTTTCAATCTAGAAGGTGCCAAGGCCCCAATGGCACACGCTTCACTGCTAGCATGAACAATGTCTCATTTGTGCTTCCAAACAACATGTCAATTCTCCAAGCTTACCAGCAAAACATTCCTGGAGTTTACACTAGTGATTTTCCAGCCAACCCGCCACGGAAATTTGATTACACTGGGAATGTCAGCCGCTCGCTTTGGCAACCCAGTTCTGGAACAAAGGGATACAAGTTGAAGTATGGGTCTAGAGTGCAGGTGGTGTTACAAGACACAAGTGCTTGGGTGATGCACTGTCACTTGGATGTTCATATCAACTTGGGTCTGGCCATGGTTTTCTTGGTGGACAATGGAGTTGGGGAGCTGCAAACAATAGAACCTCCGCCTGCAGATTTGCCTCTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

183

Amino Acids

20.32

Weight (kDa)

8.95

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018953)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0341981 RchiOBHm_Chr1g0342181
rosa_laevigata RLG00000029135
rosa_multiflora Rmu_co8426429.1_g000001 Rmu_sc0002161.1_g000002
rosa_samantha Rh1AG170300 Rh1BG135100 Rh1CG155300 Rh1CG158500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 165
AccBSI CCGCTC 1 cut(s) 339
AciI CCGC 3 cut(s) 302, 337, 527
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 3 cut(s) 22, 155, 382
AhlI ACTAGT 1 cut(s) 281
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 5 cut(s) 10, 17, 57, 251, 508
AluI AGCT 5 cut(s) 10, 17, 57, 251, 508
Alw26I GTCTC 2 cut(s) 128, 217
AoxI GGCC 2 cut(s) 172, 471
ApeKI GCWGC 2 cut(s) 10, 508
ApoI RAATTY 1 cut(s) 311
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
Asp700I GAANNNNTTC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 442
AsuNHI GCTAGC 1 cut(s) 197
BalI TGGCCA 1 cut(s) 473
BanI GGYRCC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 495
BciT130I CCWGG 1 cut(s) 270
BciVI GTATCC 1 cut(s) 365
BcoDI GTCTC 2 cut(s) 128, 217
BcuI ACTAGT 1 cut(s) 281
BfaI CTAG 5 cut(s) 69, 159, 198, 282, 393
BfmI CTRYAG 1 cut(s) 531
BfuAI ACCTGC 1 cut(s) 391
BfuI GTATCC 1 cut(s) 365
BglI GCCNNNNNGGC 1 cut(s) 180
BisI GCNGC 3 cut(s) 11, 337, 509
BlsI GCNGC 3 cut(s) 12, 338, 510
Bme1390I CCNGG 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 167, 175
BmrFI CCNGG 1 cut(s) 270
BmrI ACTGGG 2 cut(s) 333, 348
BmsI GCATC 2 cut(s) 47, 423
BmtI GCTAGC 1 cut(s) 201
BmuI ACTGGG 2 cut(s) 333, 348
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 169, 305, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 328, 354
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 3 cut(s) 169, 305, 474
BseGI GGATG 1 cut(s) 454
BseLI CCNNNNNNNGG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 328, 354
BseXI GCAGC 1 cut(s) 495
BsgI GTGCAG 1 cut(s) 419
BshFI GGCC 2 cut(s) 174, 473
BshNI GGYRCC 1 cut(s) 165
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 2 cut(s) 128, 217
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 174, 473
Bso31I GGTCTC 1 cut(s) 128
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 3 cut(s) 302, 337, 527
BspANI GGCC 2 cut(s) 174, 473
BspLI GGNNCC 2 cut(s) 167, 175
BspMAI CTGCAG 1 cut(s) 535
BspMI ACCTGC 1 cut(s) 391
BspOI GCTAGC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 165
BspTNI GGTCTC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 339
BsrI ACTGG 2 cut(s) 328, 354
BssECI CCNNGG 3 cut(s) 169, 305, 474
BssT1I CCWWGG 2 cut(s) 169, 474
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 440
Bst6I CTCTTC 1 cut(s) 113
BstC8I GCNNGC 4 cut(s) 15, 199, 341, 531
BstDEI CTNAG 2 cut(s) 51, 144
BstDSI CCRYGG 2 cut(s) 305, 474
BstF5I GGATG 1 cut(s) 454
BstMAI GTCTC 2 cut(s) 128, 217
BstMWI GCNNNNNNNGC 3 cut(s) 10, 63, 180
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 531
BstV1I GCAGC 1 cut(s) 495
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 174, 473
BtgI CCRYGG 2 cut(s) 305, 474
BtsCI GGATG 1 cut(s) 454
BtsI GCAGTG 2 cut(s) 33, 192
BtsIMutI CAGTG 4 cut(s) 33, 192, 321, 436
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 4 cut(s) 15, 199, 341, 531
Cfr13I GGNCC 1 cut(s) 173
CviAII CATG 3 cut(s) 202, 235, 475
DdeI CTNAG 2 cut(s) 51, 144
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 516
Eco130I CCWWGG 2 cut(s) 169, 474
Eco31I GGTCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 2 cut(s) 169, 474
ErhI CCWWGG 2 cut(s) 169, 474
FaeI CATG 3 cut(s) 205, 238, 478
FaiI YATR 5 cut(s) 203, 236, 387, 456, 476
FaqI GGGAC 1 cut(s) 68
FatI CATG 3 cut(s) 201, 234, 474
FauI CCCGC 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 11, 337, 509
FokI GGATG 1 cut(s) 461
Fsp4HI GCNGC 3 cut(s) 11, 337, 509
FspBI CTAG 5 cut(s) 69, 159, 198, 282, 393
GluI GCNGC 3 cut(s) 11, 337, 509
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 2 cut(s) 174, 473
Hin1II CATG 3 cut(s) 205, 238, 478
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 1 cut(s) 129
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 2 cut(s) 277, 490
Hpy188I TCNGA 2 cut(s) 62, 128
Hpy188III TCNNGA 3 cut(s) 159, 360, 393
Hpy8I GTNNAC 2 cut(s) 277, 490
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 55, 156
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4V TGCA 5 cut(s) 38, 400, 436, 511, 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 63, 180
HpyF3I CTNAG 2 cut(s) 51, 144
Hsp92II CATG 3 cut(s) 205, 238, 478
LmnI GCTCC 1 cut(s) 505
Lsp1109I GCAGC 1 cut(s) 495
LweI GCATC 2 cut(s) 47, 423
MaeI CTAG 5 cut(s) 69, 159, 198, 282, 393
MaeIII GTNAC 2 cut(s) 408, 440
MbiI CCGCTC 1 cut(s) 339
MboII GAAGA 2 cut(s) 100, 103
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 2 cut(s) 240, 311
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 1 cut(s) 123
MnlI CCTC 3 cut(s) 116, 534, 552
Mox20I TGGCCA 1 cut(s) 473
MroXI GAANNNNTTC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 473
MseI TTAA 1 cut(s) 550
MslI CAYNNNNRTG 1 cut(s) 447
Msp20I TGGCCA 1 cut(s) 473
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 10, 63, 180
NcoI CCATGG 1 cut(s) 474
NheI GCTAGC 1 cut(s) 197
NlaIII CATG 3 cut(s) 205, 238, 478
NlaIV GGNNCC 2 cut(s) 167, 175
NmuCI GTSAC 1 cut(s) 440
NspI RCATGY 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 391
PciI ACATGT 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 268
PkrI GCNGC 3 cut(s) 12, 338, 510
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
PscI ACATGT 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 167, 175
PspPI GGNCC 1 cut(s) 173
PstI CTGCAG 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 447
SaqAI TTAA 1 cut(s) 550
SatI GCNGC 3 cut(s) 11, 337, 509
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 1 cut(s) 270
SetI ASST 9 cut(s) 12, 19, 59, 108, 167, 253, 405, 510, 526
SfaNI GCATC 2 cut(s) 47, 423
SfcI CTRYAG 1 cut(s) 531
SmiMI CAYNNNNRTG 1 cut(s) 447
SpeI ACTAGT 1 cut(s) 281
Sse9I AATT 2 cut(s) 240, 311
SsiI CCGC 3 cut(s) 302, 337, 527
SspMI CTAG 5 cut(s) 69, 159, 198, 282, 393
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 2 cut(s) 169, 474
TaaI ACNGT 1 cut(s) 440
TaqI TCGA 2 cut(s) 78, 96
TaqII GACCGA 1 cut(s) 110
TasI AATT 2 cut(s) 240, 311
TauI GCSGC 1 cut(s) 339
Tru1I TTAA 1 cut(s) 550
Tru9I TTAA 1 cut(s) 550
TscAI CASTG 4 cut(s) 40, 199, 328, 443
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 10, 508
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 218, 443
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 4 cut(s) 40, 199, 328, 443
XapI RAATTY 1 cut(s) 311
XbaI TCTAGA 2 cut(s) 158, 392
XceI RCATGY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 481
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 5 cut(s) 69, 159, 198, 282, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.