RchiOBHm_Chr1g0353521

Ripening-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016390
Physical Location & Seq
Forward (+)
46740532 .. 46740996
465 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGAAGTACCGCTTTTGTTCTTCAGGTGCTTTTCTCCTCATCTTCATCATTCTTGTGACTATCTGTTTGTGCATTGAAGCTCGAACCTACAATCCAAGTGGCAAAATAGTTGGTAAAATGCCTCCAATGTCCGCGCATACAAAAGCAACCTTGACGATCAACAGCTTCGAGAAAGGTGGTGGGGGTGGTGGACCGTCCGAATGTGACGGTAAATATCACTCCGATAACACCCTGATCGTGGCATTGTCCACCAGGTGGTATAACAATGGGAAGAGGTGTTCAAACTACATTACCATATATGGTAAAGGAAAGAGCGTGAGAGCCAAGGTTGTTGATGAATGTGACTCAACAAGAGGATGCGATGATGACATCGTTGATGCCTCTAAAGCTGTTTGGAAGGCCTTGGGAGTTAAGGAGAGCAGCAGCGACTGGGGTGAAATGAAAATATTCTGGTCTGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

16.81

Weight (kDa)

8.65

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 38 - 154 3.1e-32 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 255
AccII CGCG 1 cut(s) 134
AciI CCGC 2 cut(s) 10, 132
AcuI CTGAAG 1 cut(s) 6
AdeI CACNNNGTG 1 cut(s) 255
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 238, 255
AgsI TTSAA 2 cut(s) 77, 282
AjnI CCWGG 1 cut(s) 251
AloI GAACNNNNNNTCC 4 cut(s) 76, 108, 262, 294
AluBI AGCT 3 cut(s) 80, 165, 389
AluI AGCT 3 cut(s) 80, 165, 389
AlwNI CAGNNNCTG 1 cut(s) 429
AoxI GGCC 1 cut(s) 399
ApeKI GCWGC 2 cut(s) 420, 423
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
Asp700I GAANNNNTTC 1 cut(s) 446
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 446
AvaII GGWCC 1 cut(s) 191
BbvI GCAGC 2 cut(s) 432, 435
BciT130I CCWGG 1 cut(s) 253
BisI GCNGC 2 cut(s) 421, 424
BlsI GCNGC 2 cut(s) 422, 425
Bme1390I CCNGG 1 cut(s) 253
Bme18I GGWCC 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 191
BmrFI CCNGG 1 cut(s) 253
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 3 cut(s) 347, 367, 448
BmuI ACTGGG 1 cut(s) 439
BsaJI CCNNGG 2 cut(s) 324, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 255
Bse1I ACTGG 1 cut(s) 434
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 2 cut(s) 324, 402
BseGI GGATG 1 cut(s) 362
BseLI CCNNNNNNNGG 2 cut(s) 238, 255
BseNI ACTGG 1 cut(s) 434
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 2 cut(s) 432, 435
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 1 cut(s) 401
BslI CCNNNNNNNGG 2 cut(s) 238, 255
BsnI GGCC 1 cut(s) 401
Bsp143I GATC 2 cut(s) 156, 234
BspACI CCGC 2 cut(s) 10, 132
BspANI GGCC 1 cut(s) 401
BspFNI CGCG 1 cut(s) 134
BsrI ACTGG 1 cut(s) 434
BssECI CCNNGG 2 cut(s) 324, 402
BssMI GATC 2 cut(s) 156, 234
BssT1I CCWWGG 2 cut(s) 324, 402
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 2 cut(s) 195, 209
Bst6I CTCTTC 1 cut(s) 266
BstF5I GGATG 1 cut(s) 362
BstFNI CGCG 1 cut(s) 134
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 2 cut(s) 159, 237
BstMBI GATC 2 cut(s) 156, 234
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 251
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 2 cut(s) 432, 435
BsuRI GGCC 1 cut(s) 401
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 1 cut(s) 362
CaiI CAGNNNCTG 1 cut(s) 429
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 191
CsiI ACCWGGT 1 cut(s) 251
Csp6I GTAC 1 cut(s) 7
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviJI RGCY 5 cut(s) 80, 165, 323, 389, 401
CviKI_1 RGCY 5 cut(s) 80, 165, 323, 389, 401
CviQI GTAC 1 cut(s) 7
DpnI GATC 2 cut(s) 158, 236
DpnII GATC 2 cut(s) 156, 234
DraIII CACNNNGTG 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Eco130I CCWWGG 2 cut(s) 324, 402
Eco147I AGGCCT 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 251
EcoT14I CCWWGG 2 cut(s) 324, 402
ErhI CCWWGG 2 cut(s) 324, 402
FaiI YATR 5 cut(s) 138, 261, 296, 298, 300
FalI AAGNNNNNCTT 1 cut(s) 28
Fnu4HI GCNGC 2 cut(s) 421, 424
FokI GGATG 1 cut(s) 369
Fsp4HI GCNGC 2 cut(s) 421, 424
GlaI GCGC 1 cut(s) 135
GluI GCNGC 2 cut(s) 421, 424
HaeIII GGCC 1 cut(s) 401
HhaI GCGC 1 cut(s) 136
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HinfI GANTC 1 cut(s) 344
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 2 cut(s) 191, 249
Hpy188I TCNGA 3 cut(s) 199, 223, 457
Hpy188III TCNNGA 1 cut(s) 169
Hpy8I GTNNAC 2 cut(s) 191, 249
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 2 cut(s) 195, 209
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HspAI GCGC 1 cut(s) 134
Kzo9I GATC 2 cut(s) 156, 234
LpnPI CCDG 6 cut(s) 9, 238, 245, 265, 415, 436
Lsp1109I GCAGC 2 cut(s) 432, 435
LweI GCATC 3 cut(s) 347, 367, 448
MabI ACCWGGT 1 cut(s) 251
MaeIII GTNAC 3 cut(s) 55, 203, 341
MalI GATC 2 cut(s) 158, 236
MboI GATC 2 cut(s) 156, 234
MboII GAAGA 3 cut(s) 12, 34, 283
MlyI GAGTC 1 cut(s) 338
MnlI CCTC 5 cut(s) 47, 132, 267, 347, 391
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 53
MspR9I CCNGG 1 cut(s) 253
MvaI CCWGG 1 cut(s) 253
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 386
NdeII GATC 2 cut(s) 156, 234
NmuCI GTSAC 3 cut(s) 55, 203, 341
PceI AGGCCT 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 446
PflMI CCANNNNNTGG 1 cut(s) 255
PkrI GCNGC 2 cut(s) 422, 425
PleI GAGTC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 251
PspGI CCWGG 1 cut(s) 251
PspPI GGNCC 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 429
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 1 cut(s) 53
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 2 cut(s) 421, 424
Sau3AI GATC 2 cut(s) 156, 234
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 253
SexAI ACCWGGT 1 cut(s) 251
SfaNI GCATC 3 cut(s) 347, 367, 448
SinI GGWCC 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 53
SseBI AGGCCT 1 cut(s) 401
SsiI CCGC 2 cut(s) 10, 132
SspI AATATT 1 cut(s) 447
StuI AGGCCT 1 cut(s) 401
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 2 cut(s) 324, 402
TaaI ACNGT 2 cut(s) 195, 209
TaqI TCGA 2 cut(s) 82, 168
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TseFI GTSAC 3 cut(s) 55, 203, 341
TseI GCWGC 2 cut(s) 420, 423
Tsp45I GTSAC 3 cut(s) 55, 203, 341
TspDTI ATGAA 4 cut(s) 17, 34, 351, 455
Van91I CCANNNNNTGG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 191
XmnI GAANNNNTTC 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.