RLG00000028243

Ripening-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016390
Physical Location & Seq
Forward (+)
20489711 .. 20490148
438 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028243

Sequence Viewer

Length: 438 bp
ATGATTTCATTGTGCAGGGGAACAGCACTCCTTGTGACAATCGGCTTGATCATTGAAGCTCGAACCTACAATCCAAGTGGCAAAATAGTTGGTAAAAAGCCTCCGGTGTCCGGGCGTACAAAAGGAACCTTGACGATCAACAGCTTCGAGAAAGGTGGTGGCCCGTCCGAATGTGACGGTAAATACCACTCCAATAACACCCCGATCGTGGCATTGTCCACCAGGTGGTACAATAATGGGAAGAGATGGTGTTCAAACTACATTACCATATATGGTAATGGAAAGAGTGTGAAAGCCAAGGTTGTTGATGAGTGTGACTCAACAAGAGGATGTGATGATGACATCGTTGATGCCTCTAAAGCTGTTTGGAAGGCCTTGGGAGTTGAGGAAAGCGGCAGCGACTGGGGTGAAATGAAAATATTCCGGTCTGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.68

Weight (kDa)

8.8

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 31 - 144 3e-26 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 225
AciI CCGC 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 225
AfaI GTAC 2 cut(s) 118, 230
AfiI CCNNNNNNNGG 3 cut(s) 110, 208, 225
AgsI TTSAA 2 cut(s) 56, 255
AjnI CCWGG 1 cut(s) 221
AloI GAACNNNNNNTCC 2 cut(s) 55, 87
AluBI AGCT 3 cut(s) 59, 144, 362
AluI AGCT 3 cut(s) 59, 144, 362
AlwNI CAGNNNCTG 1 cut(s) 402
AoxI GGCC 2 cut(s) 160, 372
ApeKI GCWGC 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 161
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 419
BbvI GCAGC 1 cut(s) 408
BccI CCATC 1 cut(s) 240
BciT130I CCWGG 1 cut(s) 223
BclI TGATCA 1 cut(s) 48
BcnI CCSGG 1 cut(s) 112
BisI GCNGC 2 cut(s) 394, 397
BlsI GCNGC 2 cut(s) 395, 398
Bme1390I CCNGG 2 cut(s) 112, 223
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 112, 223
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 2 cut(s) 340, 421
BmuI ACTGGG 1 cut(s) 412
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 297, 375
BsaWI WCCGGW 2 cut(s) 103, 423
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 208, 225
Bse1I ACTGG 1 cut(s) 407
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 2 cut(s) 297, 375
BseGI GGATG 1 cut(s) 335
BseLI CCNNNNNNNGG 3 cut(s) 110, 208, 225
BseNI ACTGG 1 cut(s) 407
BseXI GCAGC 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 2 cut(s) 162, 374
BsiEI CGRYCG 1 cut(s) 207
BsiSI CCGG 3 cut(s) 104, 111, 424
BslI CCNNNNNNNGG 3 cut(s) 110, 208, 225
BsnI GGCC 2 cut(s) 162, 374
Bsp143I GATC 3 cut(s) 48, 135, 204
BspACI CCGC 1 cut(s) 393
BspANI GGCC 2 cut(s) 162, 374
BspLI GGNNCC 1 cut(s) 127
BsrI ACTGG 1 cut(s) 407
BssECI CCNNGG 2 cut(s) 297, 375
BssMI GATC 3 cut(s) 48, 135, 204
BssT1I CCWWGG 2 cut(s) 297, 375
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 1 cut(s) 179
Bst6I CTCTTC 1 cut(s) 236
BstF5I GGATG 1 cut(s) 335
BstKTI GATC 3 cut(s) 51, 138, 207
BstMBI GATC 3 cut(s) 48, 135, 204
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 1 cut(s) 223
BstSCI CCNGG 2 cut(s) 110, 221
BstV1I GCAGC 1 cut(s) 408
BsuRI GGCC 2 cut(s) 162, 374
BtsCI GGATG 1 cut(s) 335
CaiI CAGNNNCTG 1 cut(s) 402
Cfr13I GGNCC 1 cut(s) 161
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 2 cut(s) 117, 229
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviJI RGCY 8 cut(s) 45, 59, 100, 144, 162, 296, 362, 374
CviKI_1 RGCY 8 cut(s) 45, 59, 100, 144, 162, 296, 362, 374
CviQI GTAC 2 cut(s) 117, 229
DpnI GATC 3 cut(s) 50, 137, 206
DpnII GATC 3 cut(s) 48, 135, 204
DraIII CACNNNGTG 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Eco130I CCWWGG 2 cut(s) 297, 375
Eco147I AGGCCT 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 221
EcoT14I CCWWGG 2 cut(s) 297, 375
ErhI CCWWGG 2 cut(s) 297, 375
FaiI YATR 3 cut(s) 269, 271, 273
FbaI TGATCA 1 cut(s) 48
Fnu4HI GCNGC 2 cut(s) 394, 397
FokI GGATG 1 cut(s) 342
Fsp4HI GCNGC 2 cut(s) 394, 397
GluI GCNGC 2 cut(s) 394, 397
HaeIII GGCC 2 cut(s) 162, 374
HapII CCGG 3 cut(s) 104, 111, 424
HinfI GANTC 1 cut(s) 317
HpaII CCGG 3 cut(s) 104, 111, 424
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 2 cut(s) 169, 430
Hpy188III TCNNGA 1 cut(s) 148
Hpy8I GTNNAC 1 cut(s) 219
HpyAV CCTTC 1 cut(s) 364
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 1 cut(s) 15
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 3 cut(s) 48, 135, 204
LpnPI CCDG 5 cut(s) 117, 124, 208, 235, 388
Lsp1109I GCAGC 1 cut(s) 408
LweI GCATC 2 cut(s) 340, 421
MabI ACCWGGT 1 cut(s) 221
MaeIII GTNAC 3 cut(s) 34, 173, 314
MalI GATC 3 cut(s) 50, 137, 206
MboI GATC 3 cut(s) 48, 135, 204
MboII GAAGA 1 cut(s) 253
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 4 cut(s) 111, 320, 364, 379
MroXI GAANNNNTTC 1 cut(s) 419
MspI CCGG 3 cut(s) 104, 111, 424
MspR9I CCNGG 2 cut(s) 112, 223
MvaI CCWGG 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 359
NciI CCSGG 1 cut(s) 112
NdeII GATC 3 cut(s) 48, 135, 204
NlaIV GGNNCC 1 cut(s) 127
NmuCI GTSAC 3 cut(s) 34, 173, 314
PceI AGGCCT 1 cut(s) 374
PdmI GAANNNNTTC 1 cut(s) 419
PflMI CCANNNNNTGG 1 cut(s) 225
PkrI GCNGC 2 cut(s) 395, 398
Ple19I CGATCG 1 cut(s) 207
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 161
PstNI CAGNNNCTG 1 cut(s) 402
PvuI CGATCG 1 cut(s) 207
RsaI GTAC 2 cut(s) 118, 230
RsaNI GTAC 2 cut(s) 117, 229
SatI GCNGC 2 cut(s) 394, 397
Sau3AI GATC 3 cut(s) 48, 135, 204
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 112, 223
SetI ASST 8 cut(s) 61, 68, 131, 146, 157, 227, 303, 364
SexAI ACCWGGT 1 cut(s) 221
SfaNI GCATC 2 cut(s) 340, 421
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 1 cut(s) 393
SspI AATATT 1 cut(s) 420
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 2 cut(s) 110, 221
StyI CCWWGG 2 cut(s) 297, 375
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 61, 147
TauI GCSGC 1 cut(s) 396
TseFI GTSAC 3 cut(s) 34, 173, 314
TseI GCWGC 1 cut(s) 396
Tsp45I GTSAC 3 cut(s) 34, 173, 314
TspDTI ATGAA 1 cut(s) 428
Van91I CCANNNNNTGG 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.