RchiOBHm_Chr1g0369021

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018043
Physical Location & Seq
Reverse (-)
58945337 .. 58947325
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
ATGGACCCATCGAGCAAGGAGAAGTATCGTCCCAATTTGGACACAGTTCTAGAGGTCCCTCACAAGATCACAGTTCTAAAGGTCCCTTGCAAGAACACAACAAAGTTATGGAACAGAATGAAATCATTGATGAACCCGAGGGCACTGTCTCCCGATGTCATTGATGAACCCGATGGCAGGGCACTAGCTAGCTTACTTAGAGTTATTGGAGCTCCACTCGACCGCAACCTCATCCTAGGGCAATGGGATCATTGGGATGCCGGGGCTGAATACATTGTGAAGCAATACGTGGCGGCGTTGGGCGGGGAGAAGGCTTTGCCATCAATGGACAGCTTGTGTGCGACGGGCAAGGTGCAGATCACAGTGTCAAATGGGACGAAAGGAAGCTTGAAATCCATTGGCGGTGGGGGAGGAGTCGGAGGACATGGAATGCCGGGGTTTGTGCTCTGGCAAAAGAGGGAGCTATGGTGCTTGGAAGTGATGTTGTCTACTGGTTACAAAGTCAGCACGGGGAGTGATGGCAAGGCGTCTTTGCCTTGGCATCACTCGTACGCTTATCGAGTCCCATCTCTTCCCCTCGGACATCTCCTTCAGGGTCTTAATCCAAGGTCAACAGCAAATTTATTCAGAAACTCCGTTTGTTCTGCGGACAAACCAATAAACAAAGAGGACTGCTTCATACTAAAACTGAAGGTTAAACCTTCAACACTAAGCAGCAGCAACGTAGAGATAGTTCGACAAACCGTTTTGGGCTACTTCAGCCACAAAACAGGCCTTTTGGTGAAGCTAGAAGACTCTCGCCAACTAAGGACGACAGCTTTCGGAAAGCACAGTTATTGGGAATCGACAATGGAGTCAGACTTCCAAGACTACAGAACCATCGACGGCCTCAACATCGCACACGGGGGCAGGACTTGTGTGTCTTTGTGTGGTGAGAGCTCGGAAGGCCATCCCCGGAGAAGAATAGTGAAATTGGTTTGGGCACTTGAAGAAGTAGGTTTTGACATTAAGGGGTTGTCCGTGGAGTGCTTCTTGCCTCCTGGTGACTTAAAGAAAGAGGAAGAAAGCTGCGGGATGGTGAAATCTAAATCTTGTGCAAAATTAACATCCAGAATTCCATCTCCTTTGAGATTTCAAGCTACCAAAGTAGTTCCCAGTGATGATTCTAATCATGATCTAGAAGGTAGCGACATTAGTGACCAGGAGGATCTTCAAGATTTCGAGAGCTGCAACAGTAGTGACCTTGATGATTCTGATCAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

421

Amino Acids

46.25

Weight (kDa)

6.83

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 113 - 347 1.1e-68 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018043)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14750
rosa_chinensis RchiOBHm_Chr1g0369021
rosa_laevigata RLG00000027164
rosa_multiflora Rmu_sc0004250.1_g000030
rosa_roxburghii Rroxscaffold_4G00288080
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG353900 Rh1BG317400 Rh1CG331900 Rh1DG348300
rosa_wichuraiana Rw1G031270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 853, 919
AccI GTMKAC 1 cut(s) 488
AciI CCGC 6 cut(s) 223, 293, 303, 402, 647, 1071
AclWI GGATC 2 cut(s) 255, 1215
AcsI RAATTY 2 cut(s) 619, 1113
AcuI CTGAAG 3 cut(s) 575, 710, 742
AcyI GRCGYC 1 cut(s) 527
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 5 cut(s) 391, 705, 989, 1136, 1214
AjnI CCWGG 2 cut(s) 1039, 1200
Alw21I GWGCWC 3 cut(s) 214, 447, 941
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 2 cut(s) 255, 1215
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 3 cut(s) 772, 886, 946
ApeKI GCWGC 4 cut(s) 714, 717, 1068, 1227
ApoI RAATTY 2 cut(s) 619, 1113
AspA2I CCTAGG 1 cut(s) 235
AspS9I GGNCC 3 cut(s) 4, 55, 82
AsuC2I CCSGG 3 cut(s) 262, 435, 955
AsuHPI GGTGA 4 cut(s) 793, 944, 1055, 1090
AsuNHI GCTAGC 1 cut(s) 188
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 3 cut(s) 4, 55, 82
AvrII CCTAGG 1 cut(s) 235
BaeGI GKGCMC 3 cut(s) 145, 184, 985
BanII GRGCYC 2 cut(s) 214, 941
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 3 cut(s) 214, 447, 941
BbvI GCAGC 4 cut(s) 726, 729, 1055, 1214
BccI CCATC 9 cut(s) 16, 167, 328, 512, 574, 887, 957, 1069, 1126
BceAI ACGGC 1 cut(s) 901
BciT130I CCWGG 2 cut(s) 1041, 1202
BclI TGATCA 1 cut(s) 1255
BcnI CCSGG 3 cut(s) 262, 435, 955
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 6 cut(s) 50, 185, 189, 236, 788, 1178
BfmI CTRYAG 1 cut(s) 871
BisI GCNGC 5 cut(s) 294, 715, 718, 1069, 1228
BlnI CCTAGG 1 cut(s) 235
BlsI GCNGC 5 cut(s) 295, 716, 719, 1070, 1229
Bme1390I CCNGG 5 cut(s) 262, 435, 955, 1041, 1202
Bme18I GGWCC 3 cut(s) 4, 55, 82
BmeT110I CYCGRG 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 4, 55, 82
BmiI GGNNCC 3 cut(s) 6, 57, 84
BmrFI CCNGG 5 cut(s) 262, 435, 955, 1041, 1202
BmrI ACTGGG 1 cut(s) 1149
BmsI GCATC 2 cut(s) 247, 550
BmtI GCTAGC 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 1149
BpiI GAAGAC 1 cut(s) 798
BplI GAGNNNNNCTC 2 cut(s) 201, 233
BpuMI CCSGG 3 cut(s) 262, 435, 955
BsaAI YACGTR 1 cut(s) 289
BsaBI GATNNNNATC 2 cut(s) 1167, 1254
BsaHI GRCGYC 1 cut(s) 527
BsaJI CCNNGG 9 cut(s) 137, 235, 261, 434, 536, 577, 605, 953, 1020
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 496, 1155
Bse3DI GCAATG 1 cut(s) 248
Bse8I GATNNNNATC 2 cut(s) 1167, 1254
BseBI CCWGG 2 cut(s) 1041, 1202
BseDI CCNNGG 9 cut(s) 137, 235, 261, 434, 536, 577, 605, 953, 1020
BseGI GGATG 5 cut(s) 231, 262, 949, 1080, 1106
BseJI GATNNNNATC 2 cut(s) 1167, 1254
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMI GCAATG 1 cut(s) 248
BseNI ACTGG 2 cut(s) 496, 1155
BseRI GAGGAG 1 cut(s) 426
BseSI GKGCMC 3 cut(s) 145, 184, 985
BseXI GCAGC 4 cut(s) 726, 729, 1055, 1214
BsgI GTGCAG 1 cut(s) 374
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 3 cut(s) 774, 888, 948
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 3 cut(s) 214, 447, 941
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 3 cut(s) 261, 434, 955
BsiWI CGTACG 1 cut(s) 549
BslFI GGGAC 5 cut(s) 15, 41, 68, 388, 548
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 1 cut(s) 153
BsmFI GGGAC 5 cut(s) 15, 41, 68, 388, 548
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 3 cut(s) 774, 888, 948
BsoBI CYCGRG 1 cut(s) 136
Bsp1286I GDGCHC 6 cut(s) 145, 184, 214, 447, 941, 985
Bsp143I GATC 6 cut(s) 66, 247, 357, 1174, 1207, 1255
BspACI CCGC 6 cut(s) 223, 293, 303, 402, 647, 1071
BspANI GGCC 3 cut(s) 774, 888, 948
BspHI TCATGA 1 cut(s) 1171
BspLI GGNNCC 3 cut(s) 6, 57, 84
BspOI GCTAGC 1 cut(s) 192
BspPI GGATC 2 cut(s) 255, 1215
BsrDI GCAATG 1 cut(s) 248
BsrI ACTGG 2 cut(s) 496, 1155
BssECI CCNNGG 9 cut(s) 137, 235, 261, 434, 536, 577, 605, 953, 1020
BssMI GATC 6 cut(s) 66, 247, 357, 1174, 1207, 1255
BssNI GRCGYC 1 cut(s) 527
BssT1I CCWWGG 3 cut(s) 235, 536, 605
Bst2UI CCWGG 2 cut(s) 1041, 1202
Bst4CI ACNGT 7 cut(s) 46, 73, 147, 364, 745, 833, 1235
Bst6I CTCTTC 1 cut(s) 576
BstACI GRCGYC 1 cut(s) 527
BstBAI YACGTR 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 3 cut(s) 197, 710, 806
BstDSI CCRYGG 1 cut(s) 1020
BstF5I GGATG 5 cut(s) 231, 262, 949, 1080, 1106
BstKTI GATC 6 cut(s) 69, 250, 360, 1177, 1210, 1258
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 6 cut(s) 66, 247, 357, 1174, 1207, 1255
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 2 cut(s) 759, 945
BstNI CCWGG 2 cut(s) 1041, 1202
BstSCI CCNGG 5 cut(s) 260, 433, 953, 1039, 1200
BstSFI CTRYAG 1 cut(s) 871
BstSLI GKGCMC 3 cut(s) 145, 184, 985
BstV1I GCAGC 4 cut(s) 726, 729, 1055, 1214
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 1 cut(s) 1207
BstYI RGATCY 1 cut(s) 1207
BsuRI GGCC 3 cut(s) 774, 888, 948
BtgI CCRYGG 1 cut(s) 1020
BtgZI GCGATG 1 cut(s) 880
BtsCI GGATG 5 cut(s) 231, 262, 949, 1080, 1106
BtsIMutI CAGTG 3 cut(s) 143, 369, 1162
Cac8I GCNNGC 1 cut(s) 190
CciI TCATGA 1 cut(s) 1171
Cfr13I GGNCC 3 cut(s) 4, 55, 82
CseI GACGC 1 cut(s) 516
Csp6I GTAC 1 cut(s) 550
CviAII CATG 2 cut(s) 425, 1172
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 3 cut(s) 197, 710, 806
DpnI GATC 6 cut(s) 68, 249, 359, 1176, 1209, 1257
DpnII GATC 6 cut(s) 66, 247, 357, 1174, 1207, 1255
DrdI GACNNNNNNGTC 2 cut(s) 853, 919
DseDI GACNNNNNNGTC 2 cut(s) 853, 919
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Ecl136II GAGCTC 2 cut(s) 212, 939
Eco130I CCWWGG 3 cut(s) 235, 536, 605
Eco147I AGGCCT 1 cut(s) 774
Eco24I GRGCYC 2 cut(s) 214, 941
Eco47I GGWCC 3 cut(s) 4, 55, 82
Eco53kI GAGCTC 2 cut(s) 212, 939
Eco57I CTGAAG 3 cut(s) 575, 710, 742
Eco88I CYCGRG 1 cut(s) 136
EcoICRI GAGCTC 2 cut(s) 212, 939
EcoO109I RGGNCCY 2 cut(s) 55, 82
EcoRI GAATTC 1 cut(s) 1113
EcoRII CCWGG 2 cut(s) 1039, 1200
EcoT14I CCWWGG 3 cut(s) 235, 536, 605
EcoT38I GRGCYC 2 cut(s) 214, 941
ErhI CCWWGG 3 cut(s) 235, 536, 605
FaeI CATG 2 cut(s) 428, 1175
FaiI YATR 5 cut(s) 109, 426, 466, 680, 1173
FaqI GGGAC 5 cut(s) 15, 41, 68, 388, 548
FatI CATG 2 cut(s) 424, 1171
FauI CCCGC 2 cut(s) 296, 1064
FbaI TGATCA 1 cut(s) 1255
FblI GTMKAC 1 cut(s) 488
Fnu4HI GCNGC 5 cut(s) 294, 715, 718, 1069, 1228
FokI GGATG 5 cut(s) 218, 269, 936, 1087, 1093
FriOI GRGCYC 2 cut(s) 214, 941
Fsp4HI GCNGC 5 cut(s) 294, 715, 718, 1069, 1228
FspBI CTAG 6 cut(s) 50, 185, 189, 236, 788, 1178
GluI GCNGC 5 cut(s) 294, 715, 718, 1069, 1228
HaeIII GGCC 3 cut(s) 774, 888, 948
HapII CCGG 3 cut(s) 261, 434, 955
HgaI GACGC 1 cut(s) 516
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 2 cut(s) 428, 1175
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HindIII AAGCTT 1 cut(s) 385
HinfI GANTC 7 cut(s) 414, 561, 794, 842, 854, 1163, 1250
HpaII CCGG 3 cut(s) 261, 434, 955
HphI GGTGA 4 cut(s) 793, 944, 1055, 1090
Hpy166II GTNNAC 2 cut(s) 489, 612
Hpy188I TCNGA 7 cut(s) 419, 581, 629, 824, 859, 943, 1255
Hpy188III TCNNGA 7 cut(s) 50, 152, 1110, 1172, 1178, 1214, 1222
Hpy8I GTNNAC 2 cut(s) 489, 612
Hpy99I CGWCG 2 cut(s) 346, 887
HpyAV CCTTC 6 cut(s) 304, 599, 685, 711, 938, 1175
HpyCH4III ACNGT 7 cut(s) 46, 73, 147, 364, 745, 833, 1235
HpyCH4IV ACGT 2 cut(s) 288, 723
HpyCH4V TGCA 4 cut(s) 90, 355, 1097, 1230
HpyF10VI GCNNNNNNNGC 2 cut(s) 759, 945
HpyF3I CTNAG 3 cut(s) 197, 710, 806
HpySE526I ACGT 2 cut(s) 288, 723
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 2 cut(s) 428, 1175
Ksp22I TGATCA 1 cut(s) 1255
Kzo9I GATC 6 cut(s) 66, 247, 357, 1174, 1207, 1255
LmnI GCTCC 3 cut(s) 209, 217, 460
Lsp1109I GCAGC 4 cut(s) 726, 729, 1055, 1214
LweI GCATC 2 cut(s) 247, 550
MaeI CTAG 6 cut(s) 50, 185, 189, 236, 788, 1178
MaeII ACGT 2 cut(s) 288, 723
MaeIII GTNAC 4 cut(s) 494, 1043, 1196, 1238
MalI GATC 6 cut(s) 68, 249, 359, 1176, 1209, 1257
MboI GATC 6 cut(s) 66, 247, 357, 1174, 1207, 1255
MboII GAAGA 6 cut(s) 563, 803, 972, 1001, 1073, 1202
MflI RGATCY 1 cut(s) 1207
MhlI GDGCHC 6 cut(s) 145, 184, 214, 447, 941, 985
MluCI AATT 5 cut(s) 34, 619, 971, 1100, 1113
MlyI GAGTC 4 cut(s) 423, 570, 788, 863
MmeI TCCRAC 1 cut(s) 397
MseI TTAA 6 cut(s) 600, 696, 1008, 1049, 1103, 1264
MslI CAYNNNNRTG 1 cut(s) 255
MspI CCGG 3 cut(s) 261, 434, 955
MspR9I CCNGG 5 cut(s) 262, 435, 955, 1041, 1202
Mva1269I GAATGC 1 cut(s) 435
MvaI CCWGG 2 cut(s) 1041, 1202
MwoI GCNNNNNNNGC 2 cut(s) 759, 945
NciI CCSGG 3 cut(s) 262, 435, 955
NdeII GATC 6 cut(s) 66, 247, 357, 1174, 1207, 1255
NheI GCTAGC 1 cut(s) 188
NlaIII CATG 2 cut(s) 428, 1175
NlaIV GGNNCC 3 cut(s) 6, 57, 84
NmuCI GTSAC 3 cut(s) 1043, 1196, 1238
PagI TCATGA 1 cut(s) 1171
PceI AGGCCT 1 cut(s) 774
PctI GAATGC 1 cut(s) 435
PfeI GAWTC 3 cut(s) 842, 1163, 1250
Pfl23II CGTACG 1 cut(s) 549
PkrI GCNGC 5 cut(s) 295, 716, 719, 1070, 1229
PleI GAGTC 4 cut(s) 422, 569, 788, 862
PpsI GAGTC 4 cut(s) 422, 569, 788, 862
Ppu21I YACGTR 1 cut(s) 289
PpuMI RGGWCCY 2 cut(s) 55, 82
Psp124BI GAGCTC 2 cut(s) 214, 941
Psp5II RGGWCCY 2 cut(s) 55, 82
Psp6I CCWGG 2 cut(s) 1039, 1200
PspGI CCWGG 2 cut(s) 1039, 1200
PspLI CGTACG 1 cut(s) 549
PspN4I GGNNCC 3 cut(s) 6, 57, 84
PspPI GGNCC 3 cut(s) 4, 55, 82
PspPPI RGGWCCY 2 cut(s) 55, 82
PsrI GAACNNNNNNTAC 2 cut(s) 717, 749
PsuI RGATCY 1 cut(s) 1207
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 2 cut(s) 214, 941
SaqAI TTAA 6 cut(s) 600, 696, 1008, 1049, 1103, 1264
SatI GCNGC 5 cut(s) 294, 715, 718, 1069, 1228
Sau3AI GATC 6 cut(s) 66, 247, 357, 1174, 1207, 1255
Sau96I GGNCC 3 cut(s) 4, 55, 82
SchI GAGTC 4 cut(s) 423, 570, 788, 863
ScrFI CCNGG 5 cut(s) 262, 435, 955, 1041, 1202
SduI GDGCHC 6 cut(s) 145, 184, 214, 447, 941, 985
SfaNI GCATC 2 cut(s) 247, 550
SfcI CTRYAG 1 cut(s) 871
SinI GGWCC 3 cut(s) 4, 55, 82
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 5 cut(s) 34, 619, 971, 1100, 1113
SseBI AGGCCT 1 cut(s) 774
SsiI CCGC 6 cut(s) 223, 293, 303, 402, 647, 1071
SspMI CTAG 6 cut(s) 50, 185, 189, 236, 788, 1178
SstI GAGCTC 2 cut(s) 214, 941
StuI AGGCCT 1 cut(s) 774
StyD4I CCNGG 5 cut(s) 260, 433, 953, 1039, 1200
StyI CCWWGG 3 cut(s) 235, 536, 605
TaaI ACNGT 7 cut(s) 46, 73, 147, 364, 745, 833, 1235
TaiI ACGT 2 cut(s) 291, 726
TaqI TCGA 7 cut(s) 11, 219, 559, 736, 845, 882, 1221
TasI AATT 5 cut(s) 34, 619, 971, 1100, 1113
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 3 cut(s) 842, 1163, 1250
Tru1I TTAA 6 cut(s) 600, 696, 1008, 1049, 1103, 1264
Tru9I TTAA 6 cut(s) 600, 696, 1008, 1049, 1103, 1264
TscAI CASTG 3 cut(s) 150, 369, 1162
TseFI GTSAC 3 cut(s) 1043, 1196, 1238
TseI GCWGC 4 cut(s) 714, 717, 1068, 1227
Tsp45I GTSAC 3 cut(s) 1043, 1196, 1238
TspDTI ATGAA 4 cut(s) 134, 146, 180, 667
TspGWI ACGGA 2 cut(s) 625, 1009
TspRI CASTG 3 cut(s) 150, 369, 1162
VpaK11BI GGWCC 3 cut(s) 4, 55, 82
XapI RAATTY 2 cut(s) 619, 1113
XbaI TCTAGA 2 cut(s) 49, 1177
XmaJI CCTAGG 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 488
XspI CTAG 6 cut(s) 50, 185, 189, 236, 788, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.