RchiOBHm_Chr1g0370871

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016395
Physical Location & Seq
Forward (+)
60251918 .. 60253894
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
ATGGAGAATTCGAAACTGGTCCAAGTCTCGGAGCAAGAGGTCCGAATCGACTTCACCTTAAACCAAAAATGCCGCGCCAACGTCCGCCTGACCTCCCTGAGCTCCACGTGCCCGGTCGCCTTCAAGGTCCAGACCTCCTCCCCTCACAAGTTCCTCGTCAAGCCCCCCACCGGCCTCATCCCTCCCTTATCCTTCGCCACCTTCCAGGTCGTCCTCAAGCCCCAGTCCCACCTCCCCGCCTCCTTCCCCCGCTCCCCCGCCGACCGCTTCCTCATCAAGACCGCCGAGTTCTTCCCCTCCTCCGCCGGCAACTCCGACTCCCTCGACTCCTGGTTCAGAGGGGCCCACACTCGCGACCTCAAGCTCAAGGTCGCCTTCGTGGGCCCAGTTCTTCTGCGGCACGCCGTGCACTGCGGGGATTGCGAGGCGGTCAGGAATATAATCAGGCGGCAGAGGACGATTCTGGCGGAGCTGACGCCAGGGGAGGCCGAGTCGCTGCTCCGAGTCGCGACCGAGTTGGCCGAGCCGGAGAGGATGATTAATTTGCTGCTTGAGGCCGGGTTGAGGATCGAGGCGGGTCTGAGGTTGGATCAGGTGGATGAGGAATTGGTGCGGAACGGGATGGATGAGCTACGCGTGGCGGAGGCTTCTGATCGGTTTGCTAGTGTTCTGGGTTTGAGGGATTCGGGACCGTCGACAGCAGAAGACCCCAGGATAACTAAAGGTCATGGCCATCGTCCACGAGTTGCTACTGATAGTGATAAGCTAGAATTGGGGGAACAAATGTTGATGGCAGCGCGAATCGGGGATTTGAAAGATGTGGAACTGTTGTTTCTTGAAGGAGCAGACATTAGCTACTGTGATCAATATGGCTTGACAGCTCTTCATGCAGCAGCCATTAAAGGGCACAAGGAGGTTGTTCTGATGCTGGTCGAATTTGGGCTAGACTTGGAATCCAGAGACAGTGAAGGCCATGCGCCGCTGCATTTGGCGGTTGTGGGGGGCAACTTGGAGACGGTTGAGATGTTGATACAGAATGGGGCAAATGTGAATGCCAAGAGCAAGAGTGGGGCTACTCCTTTGTACATGGCCACAGCTATGGGGTATGATGATATCACTGAGTTTCTTATCAGCAGAGGAGGCTGA

Protein Analysis

381

Amino Acids

41.64

Weight (kDa)

5.72

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 17 - 111 8.9e-12 MSP (Major sperm protein) domain
Ank_2 PF12796 264 - 353 6.8e-20 Ankyrin repeats (3 copies)
Ank_4 PF13637 266 - 311 5e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 283 - 331 5.5e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 295 - 344 3.7e-09 Ankyrin repeats (many copies)
Ank PF00023 324 - 354 7.4e-08 Ankyrin repeat
Ank_4 PF13637 328 - 377 1.1e-09 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016395)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g24970 FvH4_7g24970
malus_domestica MD01G1157100.v1.1
prunus_persica Prupe.2G254900_v2.0.a1
pyrus_communis pycom01g17390
rosa_chinensis RchiOBHm_Chr1g0370871
rosa_laevigata RLG00000027003
rosa_multiflora Rmu_sc0009474.1_g000017
rosa_roxburghii Rroxscaffold_4G00286170
rosa_rugosa Rorug01G0360000
rosa_samantha Rh1AG369700 Rh1BG333300 Rh1CG346700 Rh1DG364500
rosa_wichuraiana Rw1G032550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 252
AccI GTMKAC 1 cut(s) 695
AccII CGCG 5 cut(s) 75, 354, 509, 636, 799
AclWI GGATC 2 cut(s) 575, 597
AcoI YGGCCR 3 cut(s) 519, 730, 1089
AcsI RAATTY 2 cut(s) 7, 935
AcvI CACGTG 1 cut(s) 108
AcyI GRCGYC 1 cut(s) 476
AdeI CACNNNGTG 1 cut(s) 406
AfaI GTAC 1 cut(s) 1085
AfiI CCNNNNNNNGG 4 cut(s) 28, 170, 564, 903
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 3 cut(s) 124, 814, 839
AjnI CCWGG 4 cut(s) 204, 329, 478, 710
AluBI AGCT 8 cut(s) 102, 364, 472, 631, 766, 855, 881, 1097
AluI AGCT 8 cut(s) 102, 364, 472, 631, 766, 855, 881, 1097
Alw21I GWGCWC 2 cut(s) 104, 411
Alw26I GTCTC 3 cut(s) 31, 954, 1007
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 2 cut(s) 575, 597
AoxI GGCC 9 cut(s) 172, 342, 382, 486, 519, 555, 730, 970, 1089
ApaI GGGCCC 2 cut(s) 346, 386
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 6 cut(s) 496, 547, 794, 890, 893, 982
ApoI RAATTY 2 cut(s) 7, 935
AseI ATTAAT 1 cut(s) 540
AspLEI GCGC 3 cut(s) 77, 799, 979
AspS9I GGNCC 8 cut(s) 19, 40, 127, 342, 343, 382, 383, 689
AsuC2I CCSGG 2 cut(s) 113, 559
AsuHPI GGTGA 1 cut(s) 46
AsuII TTCGAA 1 cut(s) 11
AvaII GGWCC 4 cut(s) 19, 40, 127, 689
BaeGI GKGCMC 5 cut(s) 113, 346, 386, 411, 909
BalI TGGCCA 2 cut(s) 732, 1091
BanII GRGCYC 3 cut(s) 104, 346, 386
BauI CACGAG 1 cut(s) 741
BbrPI CACGTG 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 711
Bbv12I GWGCWC 2 cut(s) 104, 411
BbvI GCAGC 6 cut(s) 483, 534, 806, 902, 905, 969
BccI CCATC 3 cut(s) 616, 741, 784
BceAI ACGGC 1 cut(s) 389
BciT130I CCWGG 4 cut(s) 206, 331, 480, 712
BclI TGATCA 1 cut(s) 862
BcnI CCSGG 2 cut(s) 113, 559
BcoDI GTCTC 3 cut(s) 31, 954, 1007
BfaI CTAG 3 cut(s) 663, 767, 944
Bme1390I CCNGG 6 cut(s) 113, 206, 331, 480, 559, 712
Bme18I GGWCC 4 cut(s) 19, 40, 127, 689
BmgT120I GGNCC 8 cut(s) 19, 40, 127, 342, 343, 382, 383, 689
BmiI GGNNCC 4 cut(s) 343, 344, 384, 690
BmrFI CCNGG 6 cut(s) 113, 206, 331, 480, 559, 712
BmrI ACTGGG 2 cut(s) 217, 380
BmsI GCATC 1 cut(s) 915
BmuI ACTGGG 2 cut(s) 217, 380
BpiI GAAGAC 1 cut(s) 711
Bpu10I CCTNAGC 1 cut(s) 98
Bpu14I TTCGAA 1 cut(s) 11
BpuEI CTTGAG 4 cut(s) 200, 344, 350, 572
BpuMI CCSGG 2 cut(s) 113, 559
BsaAI YACGTR 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 476
BsaJI CCNNGG 2 cut(s) 479, 710
BsaXI ACNNNNNCTCC 4 cut(s) 302, 332, 476, 506
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 170, 564, 903
Bse118I RCCGGY 2 cut(s) 170, 305
Bse1I ACTGG 3 cut(s) 21, 223, 386
BseBI CCWGG 4 cut(s) 206, 331, 480, 712
BseDI CCNNGG 2 cut(s) 479, 710
BseGI GGATG 5 cut(s) 177, 540, 604, 627, 631
BseLI CCNNNNNNNGG 4 cut(s) 28, 170, 564, 903
BseMII CTCAG 3 cut(s) 89, 572, 1110
BseNI ACTGG 3 cut(s) 21, 223, 386
BseRI GAGGAG 2 cut(s) 127, 289
BseSI GKGCMC 5 cut(s) 113, 346, 386, 411, 909
BseXI GCAGC 6 cut(s) 483, 534, 806, 902, 905, 969
Bsh1236I CGCG 5 cut(s) 75, 354, 509, 636, 799
Bsh1285I CGRYCG 3 cut(s) 117, 265, 513
BshFI GGCC 9 cut(s) 174, 344, 384, 488, 521, 557, 732, 972, 1091
BsiEI CGRYCG 3 cut(s) 117, 265, 513
BsiHKAI GWGCWC 2 cut(s) 104, 411
BsiSI CCGG 5 cut(s) 113, 171, 306, 527, 558
BslFI GGGAC 2 cut(s) 211, 702
BslI CCNNNNNNNGG 4 cut(s) 28, 170, 564, 903
BsmAI GTCTC 3 cut(s) 31, 954, 1007
BsmBI CGTCTC 1 cut(s) 1007
BsmFI GGGAC 2 cut(s) 211, 702
BsmI GAATGC 1 cut(s) 1057
BsnI GGCC 9 cut(s) 174, 344, 384, 488, 521, 557, 732, 972, 1091
Bsp119I TTCGAA 1 cut(s) 11
Bsp120I GGGCCC 2 cut(s) 342, 382
Bsp1286I GDGCHC 6 cut(s) 104, 113, 346, 386, 411, 909
Bsp1407I TGTACA 1 cut(s) 1083
Bsp143I GATC 4 cut(s) 567, 589, 652, 862
Bsp68I TCGCGA 2 cut(s) 354, 509
BspANI GGCC 9 cut(s) 174, 344, 384, 488, 521, 557, 732, 972, 1091
BspCNI CTCAG 3 cut(s) 90, 573, 1111
BspFNI CGCG 5 cut(s) 75, 354, 509, 636, 799
BspLI GGNNCC 4 cut(s) 343, 344, 384, 690
BspPI GGATC 2 cut(s) 575, 597
BspQI GCTCTTC 1 cut(s) 888
BspT104I TTCGAA 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 252
BsrFI RCCGGY 2 cut(s) 170, 305
BsrGI TGTACA 1 cut(s) 1083
BsrI ACTGG 3 cut(s) 21, 223, 386
BssAI RCCGGY 2 cut(s) 170, 305
BssECI CCNNGG 2 cut(s) 479, 710
BssMI GATC 4 cut(s) 567, 589, 652, 862
BssNI GRCGYC 1 cut(s) 476
BssSI CACGAG 1 cut(s) 741
Bst2BI CACGAG 1 cut(s) 741
Bst2UI CCWGG 4 cut(s) 206, 331, 480, 712
Bst4CI ACNGT 5 cut(s) 693, 828, 860, 965, 1018
Bst6I CTCTTC 1 cut(s) 888
BstACI GRCGYC 1 cut(s) 476
BstAPI GCANNNNNTGC 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 1083
BstBAI YACGTR 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 307, 402
BstDEI CTNAG 3 cut(s) 98, 581, 1119
BstF5I GGATG 5 cut(s) 177, 540, 604, 627, 631
BstFNI CGCG 5 cut(s) 75, 354, 509, 636, 799
BstHHI GCGC 3 cut(s) 77, 799, 979
BstKTI GATC 4 cut(s) 570, 592, 655, 865
BstMAI GTCTC 3 cut(s) 31, 954, 1007
BstMBI GATC 4 cut(s) 567, 589, 652, 862
BstMCI CGRYCG 3 cut(s) 117, 265, 513
BstMWI GCNNNNNNNGC 5 cut(s) 108, 406, 420, 887, 1140
BstNI CCWGG 4 cut(s) 206, 331, 480, 712
BstSCI CCNGG 6 cut(s) 111, 204, 329, 478, 557, 710
BstSLI GKGCMC 5 cut(s) 113, 346, 386, 411, 909
BstUI CGCG 5 cut(s) 75, 354, 509, 636, 799
BstV1I GCAGC 6 cut(s) 483, 534, 806, 902, 905, 969
BstV2I GAAGAC 1 cut(s) 711
BstXI CCANNNNNNTGG 1 cut(s) 1099
BsuRI GGCC 9 cut(s) 174, 344, 384, 488, 521, 557, 732, 972, 1091
BtsCI GGATG 5 cut(s) 177, 540, 604, 627, 631
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 3 cut(s) 409, 970, 1116
BtuMI TCGCGA 2 cut(s) 354, 509
Cac8I GCNNGC 2 cut(s) 307, 402
CfoI GCGC 3 cut(s) 77, 799, 979
Cfr10I RCCGGY 2 cut(s) 170, 305
Cfr13I GGNCC 8 cut(s) 19, 40, 127, 342, 343, 382, 383, 689
CseI GACGC 1 cut(s) 484
Csp6I GTAC 1 cut(s) 1084
CviAII CATG 4 cut(s) 728, 887, 974, 1087
CviQI GTAC 1 cut(s) 1084
DdeI CTNAG 3 cut(s) 98, 581, 1119
DpnI GATC 4 cut(s) 569, 591, 654, 864
DpnII GATC 4 cut(s) 567, 589, 652, 862
DraIII CACNNNGTG 1 cut(s) 406
EaeI YGGCCR 3 cut(s) 519, 730, 1089
Eam1104I CTCTTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 888
EciI GGCGGA 4 cut(s) 74, 292, 482, 656
Ecl136II GAGCTC 1 cut(s) 102
Eco24I GRGCYC 3 cut(s) 104, 346, 386
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 4 cut(s) 19, 40, 127, 689
Eco53kI GAGCTC 1 cut(s) 102
Eco72I CACGTG 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 342
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 4 cut(s) 204, 329, 478, 710
EcoRV GATATC 1 cut(s) 1114
EcoT38I GRGCYC 3 cut(s) 104, 346, 386
Esp3I CGTCTC 1 cut(s) 1007
FaeI CATG 4 cut(s) 731, 890, 977, 1090
FaiI YATR 8 cut(s) 440, 729, 870, 888, 975, 1088, 1100, 1107
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 2 cut(s) 211, 702
FatI CATG 4 cut(s) 727, 886, 973, 1086
FauI CCCGC 5 cut(s) 244, 257, 265, 407, 568
FbaI TGATCA 1 cut(s) 862
FblI GTMKAC 1 cut(s) 695
FokI GGATG 5 cut(s) 164, 547, 611, 634, 638
FriOI GRGCYC 3 cut(s) 104, 346, 386
FspBI CTAG 3 cut(s) 663, 767, 944
GlaI GCGC 3 cut(s) 76, 798, 978
HaeIII GGCC 9 cut(s) 174, 344, 384, 488, 521, 557, 732, 972, 1091
HapII CCGG 5 cut(s) 113, 171, 306, 527, 558
HgaI GACGC 1 cut(s) 484
HhaI GCGC 3 cut(s) 77, 799, 979
Hin1I GRCGYC 1 cut(s) 476
Hin1II CATG 4 cut(s) 731, 890, 977, 1090
Hin6I GCGC 3 cut(s) 75, 797, 977
HinP1I GCGC 3 cut(s) 75, 797, 977
HincII GTYRAC 1 cut(s) 696
HindII GTYRAC 1 cut(s) 696
HinfI GANTC 9 cut(s) 45, 317, 326, 460, 491, 504, 683, 801, 953
HpaII CCGG 5 cut(s) 113, 171, 306, 527, 558
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 3 cut(s) 409, 696, 740
Hpy188I TCNGA 8 cut(s) 31, 44, 316, 338, 503, 582, 652, 924
Hpy188III TCNNGA 8 cut(s) 130, 277, 353, 433, 508, 687, 836, 957
Hpy8I GTNNAC 3 cut(s) 409, 696, 740
Hpy99I CGWCG 1 cut(s) 697
HpyAV CCTTC 7 cut(s) 130, 202, 211, 253, 385, 833, 962
HpyCH4III ACNGT 5 cut(s) 693, 828, 860, 965, 1018
HpyCH4IV ACGT 2 cut(s) 81, 107
HpyCH4V TGCA 3 cut(s) 409, 890, 985
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 406, 420, 887, 1140
HpyF3I CTNAG 3 cut(s) 98, 581, 1119
HpySE526I ACGT 2 cut(s) 81, 107
Hsp92I GRCGYC 1 cut(s) 476
Hsp92II CATG 4 cut(s) 731, 890, 977, 1090
HspAI GCGC 3 cut(s) 75, 797, 977
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 862
Kzo9I GATC 4 cut(s) 567, 589, 652, 862
LguI GCTCTTC 1 cut(s) 888
LmnI GCTCC 6 cut(s) 31, 107, 257, 469, 504, 842
Lsp1109I GCAGC 6 cut(s) 483, 534, 806, 902, 905, 969
LweI GCATC 1 cut(s) 915
MaeI CTAG 3 cut(s) 663, 767, 944
MaeII ACGT 2 cut(s) 81, 107
MalI GATC 4 cut(s) 569, 591, 654, 864
MbiI CCGCTC 1 cut(s) 252
MboI GATC 4 cut(s) 567, 589, 652, 862
MboII GAAGA 4 cut(s) 283, 383, 716, 875
MhlI GDGCHC 6 cut(s) 104, 113, 346, 386, 411, 909
MlsI TGGCCA 2 cut(s) 732, 1091
MluCI AATT 5 cut(s) 7, 541, 605, 770, 935
MluI ACGCGT 1 cut(s) 634
MluNI TGGCCA 2 cut(s) 732, 1091
MlyI GAGTC 4 cut(s) 311, 320, 500, 513
MmeI TCCRAC 2 cut(s) 339, 567
Mox20I TGGCCA 2 cut(s) 732, 1091
MroNI GCCGGC 1 cut(s) 305
MscI TGGCCA 2 cut(s) 732, 1091
MseI TTAA 3 cut(s) 59, 540, 900
MslI CAYNNNNRTG 1 cut(s) 1097
Msp20I TGGCCA 2 cut(s) 732, 1091
MspA1I CMGCKG 1 cut(s) 982
MspI CCGG 5 cut(s) 113, 171, 306, 527, 558
MspR9I CCNGG 6 cut(s) 113, 206, 331, 480, 559, 712
Mva1269I GAATGC 1 cut(s) 1057
MvaI CCWGG 4 cut(s) 206, 331, 480, 712
MvnI CGCG 5 cut(s) 75, 354, 509, 636, 799
MwoI GCNNNNNNNGC 5 cut(s) 108, 406, 420, 887, 1140
NaeI GCCGGC 1 cut(s) 307
NciI CCSGG 2 cut(s) 113, 559
NdeII GATC 4 cut(s) 567, 589, 652, 862
NgoMIV GCCGGC 1 cut(s) 305
NlaIII CATG 4 cut(s) 731, 890, 977, 1090
NlaIV GGNNCC 4 cut(s) 343, 344, 384, 690
NmeAIII GCCGAG 3 cut(s) 310, 514, 547
NruI TCGCGA 2 cut(s) 354, 509
NspV TTCGAA 1 cut(s) 11
PciSI GCTCTTC 1 cut(s) 888
PcsI WCGNNNNNNNCGW 1 cut(s) 692
PctI GAATGC 1 cut(s) 1057
PdiI GCCGGC 1 cut(s) 307
PfeI GAWTC 5 cut(s) 45, 460, 683, 801, 953
PleI GAGTC 4 cut(s) 311, 320, 499, 512
PmaCI CACGTG 1 cut(s) 108
PmlI CACGTG 1 cut(s) 108
PpsI GAGTC 4 cut(s) 311, 320, 499, 512
Ppu21I YACGTR 1 cut(s) 108
PshBI ATTAAT 1 cut(s) 540
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 4 cut(s) 204, 329, 478, 710
PspCI CACGTG 1 cut(s) 108
PspGI CCWGG 4 cut(s) 204, 329, 478, 710
PspN4I GGNNCC 4 cut(s) 343, 344, 384, 690
PspOMI GGGCCC 2 cut(s) 342, 382
PspPI GGNCC 8 cut(s) 19, 40, 127, 342, 343, 382, 383, 689
RruI TCGCGA 2 cut(s) 354, 509
RsaI GTAC 1 cut(s) 1085
RsaNI GTAC 1 cut(s) 1084
RseI CAYNNNNRTG 1 cut(s) 1097
SacI GAGCTC 1 cut(s) 104
SalI GTCGAC 1 cut(s) 694
SapI GCTCTTC 1 cut(s) 888
SaqAI TTAA 3 cut(s) 59, 540, 900
Sau3AI GATC 4 cut(s) 567, 589, 652, 862
Sau96I GGNCC 8 cut(s) 19, 40, 127, 342, 343, 382, 383, 689
SchI GAGTC 4 cut(s) 311, 320, 500, 513
ScrFI CCNGG 6 cut(s) 113, 206, 331, 480, 559, 712
SduI GDGCHC 6 cut(s) 104, 113, 346, 386, 411, 909
SfaNI GCATC 1 cut(s) 915
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 4 cut(s) 19, 40, 127, 689
SmiMI CAYNNNNRTG 1 cut(s) 1097
SmlI CTYRAG 4 cut(s) 215, 359, 365, 551
SmoI CTYRAG 4 cut(s) 215, 359, 365, 551
Sse9I AATT 5 cut(s) 7, 541, 605, 770, 935
SspMI CTAG 3 cut(s) 663, 767, 944
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 6 cut(s) 111, 204, 329, 478, 557, 710
TaaI ACNGT 5 cut(s) 693, 828, 860, 965, 1018
TaiI ACGT 2 cut(s) 84, 110
TaqI TCGA 6 cut(s) 11, 48, 324, 570, 695, 933
TaqII GACCGA 1 cut(s) 527
TasI AATT 5 cut(s) 7, 541, 605, 770, 935
TatI WGTACW 1 cut(s) 1083
TauI GCSGC 4 cut(s) 75, 400, 451, 982
TfiI GAWTC 5 cut(s) 45, 460, 683, 801, 953
Tru1I TTAA 3 cut(s) 59, 540, 900
Tru9I TTAA 3 cut(s) 59, 540, 900
TscAI CASTG 3 cut(s) 416, 970, 1123
TseI GCWGC 6 cut(s) 496, 547, 794, 890, 893, 982
TspDTI ATGAA 1 cut(s) 875
TspRI CASTG 3 cut(s) 416, 970, 1123
VneI GTGCAC 1 cut(s) 407
VpaK11BI GGWCC 4 cut(s) 19, 40, 127, 689
VspI ATTAAT 1 cut(s) 540
XapI RAATTY 2 cut(s) 7, 935
XmiI GTMKAC 1 cut(s) 695
XspI CTAG 3 cut(s) 663, 767, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.