RchiOBHm_Chr1g0373731

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013746
Physical Location & Seq
Reverse (-)
61813372 .. 61816950
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
ATGAAAACAATCAACACCAAACTCCATGGCTACCAAAACTCTGAGCTCCACCTGTTTCTTGACCCTTACATTTTTACTTCTCTTCCTGGTAAATACAACATCACAGAATTTAGTTTCACAGATAAAGCTATCAGCTTTACTGTTTTTGCTTTTTTCAGAAAGACCCAATTCTATTTCAAACAGTTCTATCGTGCTTCTTCATTACAAGTATGCTTGATTAAAGATGGTTGCTTTTTCATGGGATATCAGGTTACAGGGCACCAAACTTTGAATTCTGGGCCATATCCTGTGGTGGTGATCACTTGGCCTTTCGTAGAGGCTGTCAGAGCTGCTTGGAGGGTTGTTGATAATGGACTTTCTGCAGTTGATGCAGTTGTTGAGGGCTGTTCGACTTGTGAGGAACTCAGATGTGATGGTACAGTTGGGCCTGGTGGGAGTCCTGATGAGAATGGAGAGACGACAATCGATGCTCTGGTCATGTATGGGGACATCCATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

166

Amino Acids

18.61

Weight (kDa)

5.49

Isoelectric Point (pI)

22.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 97 - 163 1.9e-13 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 258
AcsI RAATTY 2 cut(s) 107, 271
AfaI GTAC 1 cut(s) 418
AgsI TTSAA 2 cut(s) 178, 271
AjnI CCWGG 2 cut(s) 85, 427
AluBI AGCT 4 cut(s) 46, 128, 135, 329
AluI AGCT 4 cut(s) 46, 128, 135, 329
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 1 cut(s) 449
AoxI GGCC 3 cut(s) 278, 305, 425
ApeKI GCWGC 1 cut(s) 329
ApoI RAATTY 2 cut(s) 107, 271
AspS9I GGNCC 2 cut(s) 278, 425
AsuHPI GGTGA 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 261
BanI GGYRCC 1 cut(s) 258
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 316
BccI CCATC 2 cut(s) 218, 407
BciT130I CCWGG 2 cut(s) 87, 429
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 360
BisI GCNGC 1 cut(s) 330
BlsI GCNGC 1 cut(s) 331
Bme1390I CCNGG 2 cut(s) 87, 429
BmgT120I GGNCC 2 cut(s) 278, 425
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 2 cut(s) 87, 429
BmsI GCATC 2 cut(s) 358, 457
Bsa29I ATCGAT 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 25
BseBI CCWGG 2 cut(s) 87, 429
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 1 cut(s) 25
BseGI GGATG 1 cut(s) 489
BseMII CTCAG 2 cut(s) 33, 418
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 1 cut(s) 316
BshFI GGCC 3 cut(s) 280, 307, 427
BshNI GGYRCC 1 cut(s) 258
BshVI ATCGAT 1 cut(s) 465
BsiHKAI GWGCWC 1 cut(s) 48
BsmAI GTCTC 1 cut(s) 449
BsmBI CGTCTC 1 cut(s) 449
BsnI GGCC 3 cut(s) 280, 307, 427
Bsp1286I GDGCHC 2 cut(s) 48, 261
Bsp143I GATC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 25
BspANI GGCC 3 cut(s) 280, 307, 427
BspCNI CTCAG 2 cut(s) 34, 417
BspDI ATCGAT 1 cut(s) 465
BspLI GGNNCC 1 cut(s) 260
BspMAI CTGCAG 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 25
Bst2UI CCWGG 2 cut(s) 87, 429
Bst4CI ACNGT 3 cut(s) 142, 183, 421
Bst6I CTCTTC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 42, 404
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 1 cut(s) 489
BstKTI GATC 1 cut(s) 300
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 1 cut(s) 297
BstMWI GCNNNNNNNGC 2 cut(s) 326, 368
BstNI CCWGG 2 cut(s) 87, 429
BstSCI CCNGG 2 cut(s) 85, 427
BstSFI CTRYAG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 465
BsuRI GGCC 3 cut(s) 280, 307, 427
BsuTUI ATCGAT 1 cut(s) 465
BtgI CCRYGG 1 cut(s) 25
BtsCI GGATG 1 cut(s) 489
Cfr13I GGNCC 2 cut(s) 278, 425
ClaI ATCGAT 1 cut(s) 465
Csp6I GTAC 1 cut(s) 417
CviAII CATG 3 cut(s) 26, 238, 478
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 2 cut(s) 42, 404
DpnI GATC 1 cut(s) 299
DpnII GATC 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 48
Eco32I GATATC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 85, 427
EcoRV GATATC 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 25
Esp3I CGTCTC 1 cut(s) 449
FaeI CATG 3 cut(s) 29, 241, 481
FaiI YATR 7 cut(s) 27, 211, 239, 283, 479, 483, 495
FatI CATG 3 cut(s) 25, 237, 477
FbaI TGATCA 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 330
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 330
GluI GCNGC 1 cut(s) 330
HaeIII GGCC 3 cut(s) 280, 307, 427
Hin1II CATG 3 cut(s) 29, 241, 481
HinfI GANTC 1 cut(s) 436
HphI GGTGA 1 cut(s) 307
Hpy188I TCNGA 4 cut(s) 43, 158, 326, 407
Hpy188III TCNNGA 2 cut(s) 59, 440
HpyCH4III ACNGT 3 cut(s) 142, 183, 421
HpyCH4V TGCA 2 cut(s) 362, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 368
HpyF3I CTNAG 2 cut(s) 42, 404
Hsp92II CATG 3 cut(s) 29, 241, 481
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 51
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 2 cut(s) 358, 457
MaeIII GTNAC 1 cut(s) 250
MalI GATC 1 cut(s) 299
MboI GATC 1 cut(s) 297
MboII GAAGA 2 cut(s) 74, 189
MhlI GDGCHC 2 cut(s) 48, 261
MluCI AATT 3 cut(s) 107, 167, 271
MlyI GAGTC 1 cut(s) 445
MnlI CCTC 4 cut(s) 310, 330, 373, 391
MseI TTAA 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 87, 429
MvaI CCWGG 2 cut(s) 87, 429
MwoI GCNNNNNNNGC 2 cut(s) 326, 368
NcoI CCATGG 1 cut(s) 25
NdeII GATC 1 cut(s) 297
NlaIII CATG 3 cut(s) 29, 241, 481
NlaIV GGNNCC 1 cut(s) 260
PkrI GCNGC 1 cut(s) 331
PleI GAGTC 1 cut(s) 444
PpsI GAGTC 1 cut(s) 444
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 2 cut(s) 85, 427
PspGI CCWGG 2 cut(s) 85, 427
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 2 cut(s) 278, 425
PstI CTGCAG 1 cut(s) 364
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
SacI GAGCTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 1 cut(s) 330
Sau3AI GATC 1 cut(s) 297
Sau96I GGNCC 2 cut(s) 278, 425
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 2 cut(s) 87, 429
SduI GDGCHC 2 cut(s) 48, 261
SetI ASST 7 cut(s) 48, 54, 130, 137, 252, 331, 500
SfaNI GCATC 2 cut(s) 358, 457
SfcI CTRYAG 1 cut(s) 360
Sse9I AATT 3 cut(s) 107, 167, 271
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 85, 427
StyI CCWWGG 1 cut(s) 25
TaaI ACNGT 3 cut(s) 142, 183, 421
TaqI TCGA 2 cut(s) 389, 465
TasI AATT 3 cut(s) 107, 167, 271
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseI GCWGC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 17, 189, 226
XapI RAATTY 2 cut(s) 107, 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.