Rh1DG383400

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0013746
Physical Location & Seq
Reverse (-)
60416204 .. 60417315
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG383400.1

Sequence Viewer

Length: 369 bp
ATGGCTACCAAAACTCTGAGCTCCACCTGTTTCTTGACCCTTACATTTTTACTTCTCTTCCTGGTTACAGGGCACCAAGCGTTGAATTCTGGGCCATACCCTGTGGTGGTGAGCACTTGGCCTTTCGTAGAGGCTGTCAGAACTGCTTGGAGGGCTGTTGATAATGGGCTCTCTGCAGTTGATGCAGTTGTTGAGGGCTGTTCGGCTTGTGAGGAACTCAGATGTGATGGTACAGTTGGGCCTGGTGGGAGTCCTGATGAGAATGGAGAGACGACAATCGATGCTCTGGTCATGGATGGGGTGACGATGGAGGTTGGAGCAGTTGCTGCCATGAGGTATGTCAAAGATGGAATCAAAGCTGCTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

122

Amino Acids

12.67

Weight (kDa)

4.39

Isoelectric Point (pI)

16.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 32 - 121 4.1e-22 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 72
AcsI RAATTY 1 cut(s) 85
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 1 cut(s) 85
AjnI CCWGG 2 cut(s) 60, 241
AluBI AGCT 2 cut(s) 21, 359
AluI AGCT 2 cut(s) 21, 359
Alw21I GWGCWC 2 cut(s) 23, 116
Alw26I GTCTC 1 cut(s) 263
AlwNI CAGNNNCTG 1 cut(s) 326
AoxI GGCC 3 cut(s) 92, 119, 239
ApeKI GCWGC 2 cut(s) 326, 359
ApoI RAATTY 1 cut(s) 85
AspS9I GGNCC 2 cut(s) 92, 239
AsuHPI GGTGA 2 cut(s) 121, 313
BaeGI GKGCMC 1 cut(s) 75
BanI GGYRCC 1 cut(s) 72
BanII GRGCYC 2 cut(s) 23, 171
Bbv12I GWGCWC 2 cut(s) 23, 116
BbvI GCAGC 2 cut(s) 313, 346
BccI CCATC 4 cut(s) 221, 290, 301, 341
BciT130I CCWGG 2 cut(s) 62, 243
BcoDI GTCTC 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 2 cut(s) 327, 360
BlsI GCNGC 2 cut(s) 328, 361
Bme1390I CCNGG 2 cut(s) 62, 243
BmgT120I GGNCC 2 cut(s) 92, 239
BmiI GGNNCC 1 cut(s) 74
BmrFI CCNGG 2 cut(s) 62, 243
BmsI GCATC 2 cut(s) 172, 271
Bsa29I ATCGAT 1 cut(s) 279
Bsc4I CCNNNNNNNGG 1 cut(s) 106
BseBI CCWGG 2 cut(s) 62, 243
BseCI ATCGAT 1 cut(s) 279
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMII CTCAG 2 cut(s) 8, 232
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 2 cut(s) 313, 346
BseYI CCCAGC 1 cut(s) 362
BshFI GGCC 3 cut(s) 94, 121, 241
BshNI GGYRCC 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 279
BsiHKAI GWGCWC 2 cut(s) 23, 116
BslI CCNNNNNNNGG 1 cut(s) 106
BsmAI GTCTC 1 cut(s) 263
BsmBI CGTCTC 1 cut(s) 263
BsnI GGCC 3 cut(s) 94, 121, 241
Bsp1286I GDGCHC 4 cut(s) 23, 75, 116, 171
BspANI GGCC 3 cut(s) 94, 121, 241
BspCNI CTCAG 2 cut(s) 9, 231
BspDI ATCGAT 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 74
BspMAI CTGCAG 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 62, 243
Bst4CI ACNGT 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 62
BstAPI GCANNNNNTGC 2 cut(s) 182, 326
BstDEI CTNAG 2 cut(s) 17, 218
BstF5I GGATG 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 263
BstMWI GCNNNNNNNGC 3 cut(s) 152, 182, 326
BstNI CCWGG 2 cut(s) 62, 243
BstSCI CCNGG 2 cut(s) 60, 241
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 75
BstV1I GCAGC 2 cut(s) 313, 346
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 3 cut(s) 94, 121, 241
BsuTUI ATCGAT 1 cut(s) 279
BtsCI GGATG 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 92, 239
ClaI ATCGAT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 231
CviAII CATG 2 cut(s) 292, 331
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 2 cut(s) 17, 218
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 23, 171
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 60, 241
EcoT38I GRGCYC 2 cut(s) 23, 171
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 2 cut(s) 295, 334
FaiI YATR 4 cut(s) 97, 293, 332, 339
FatI CATG 2 cut(s) 291, 330
Fnu4HI GCNGC 2 cut(s) 327, 360
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 2 cut(s) 23, 171
Fsp4HI GCNGC 2 cut(s) 327, 360
GluI GCNGC 2 cut(s) 327, 360
GsaI CCCAGC 1 cut(s) 366
HaeIII GGCC 3 cut(s) 94, 121, 241
Hin1II CATG 2 cut(s) 295, 334
HinfI GANTC 2 cut(s) 250, 351
HphI GGTGA 2 cut(s) 121, 313
Hpy188I TCNGA 3 cut(s) 18, 140, 221
Hpy188III TCNNGA 2 cut(s) 34, 254
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 2 cut(s) 176, 185
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 182, 326
HpyF3I CTNAG 2 cut(s) 17, 218
Hsp92II CATG 2 cut(s) 295, 334
LmnI GCTCC 2 cut(s) 26, 317
Lsp1109I GCAGC 2 cut(s) 313, 346
LweI GCATC 2 cut(s) 172, 271
MaeIII GTNAC 2 cut(s) 64, 301
MboII GAAGA 1 cut(s) 49
MhlI GDGCHC 4 cut(s) 23, 75, 116, 171
MluCI AATT 1 cut(s) 85
MlyI GAGTC 1 cut(s) 259
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 6 cut(s) 124, 144, 187, 205, 304, 327
MspR9I CCNGG 2 cut(s) 62, 243
MvaI CCWGG 2 cut(s) 62, 243
MwoI GCNNNNNNNGC 3 cut(s) 152, 182, 326
NlaIII CATG 2 cut(s) 295, 334
NlaIV GGNNCC 1 cut(s) 74
NmuCI GTSAC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 351
PkrI GCNGC 2 cut(s) 328, 361
PleI GAGTC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 60, 241
PspFI CCCAGC 1 cut(s) 362
PspGI CCWGG 2 cut(s) 60, 241
PspN4I GGNNCC 1 cut(s) 74
PspPI GGNCC 2 cut(s) 92, 239
PstI CTGCAG 1 cut(s) 178
PstNI CAGNNNCTG 1 cut(s) 326
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SacI GAGCTC 1 cut(s) 23
SatI GCNGC 2 cut(s) 327, 360
Sau96I GGNCC 2 cut(s) 92, 239
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 2 cut(s) 62, 243
SduI GDGCHC 4 cut(s) 23, 75, 116, 171
SetI ASST 5 cut(s) 23, 29, 315, 338, 361
SfaNI GCATC 2 cut(s) 172, 271
SfcI CTRYAG 1 cut(s) 174
Sse9I AATT 1 cut(s) 85
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 60, 241
TaaI ACNGT 1 cut(s) 235
TaqI TCGA 1 cut(s) 279
TasI AATT 1 cut(s) 85
TfiI GAWTC 1 cut(s) 351
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 2 cut(s) 326, 359
Tsp45I GTSAC 1 cut(s) 301
XapI RAATTY 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.