Rorug01G0379400

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013746
Physical Location & Seq
Reverse (-)
49237398 .. 49240122
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0379400.1

Sequence Viewer

Length: 939 bp
ATGAGTGGTGGACTTCACAACAACCCAAAGGGGCTCCCGGTTAATGACCCAGAGAGTACTAGAATTGAAGGGGGAGCTATATTGCAGTCTTTCAGCTTGAAGATCTTCTGCTATAATGAACTGAAAAAAGCCACCAGGAACTTCCGTTTTAAAAATATTGTGGGTTTTGGTGATTCTAATACAGTCTACAAGGGATGTATTGATGAGCATTCACTCAAGGCCACCAAGCCTGGTACCGGCATAGACATTGCCATAAAGATGCCTAGCAGAAAAGAGAGGTTCCGGCTGCAGGATCACCAGGACTTGTTGGTGACAGAATTTAACCGCCTTGGACAGCTTATTCATCCAAATCTTGTAAAAATGATTGGCTACTCTCTAGAGGATAACAAAGGGCTCCTGGTGTACGAATTTATGCCTCGAGGCAGCTTGGGAGTACATCTACATTCAAGCAAAAATGATATACGGGGACTTATTCTTCTTGATTCAAACTATGAAGCCAAAATCTATGGTTATGGCTTGGTAGAGGATGATGAGCTTGAAGGTTATAGAAGGCTTAGAGGCCATGACACTGAATGCTCTCTGGATTCATCGTACGCAGCTCCTGAGTATCTTACGGACACACACAGAGGAGAATATGAGTATAGATTTGAAAGCAGCGATGATGATGAAATGGGCCAGTACTCCAAAGGTGGAGCTATTAGAGCAGCTAAGCTTGTAGCTCAATGCTTATCAATAGATCCCAAGGGAAGGCCAAACATGAATGATGTGGTAAAAGCATTGGAGCAGCTTCAGGAATCCAGTGACATGGAGGGCTTGGGAATTTGTGAAGATGAACCTTCCTGCAGAAATCACAGGAGGAAGTATCAATCAGAAAGGCAATGCAATAACTGCTTCTCATTCCAGGCAATCTGTTTGCGGCAGCCATGCATAAGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

312

Amino Acids

35.37

Weight (kDa)

6.22

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 49 - 154 1.6e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 417
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AccI GTMKAC 1 cut(s) 186
AciI CCGC 2 cut(s) 325, 916
AclWI GGATC 2 cut(s) 300, 731
AcsI RAATTY 3 cut(s) 317, 407, 819
AcuI CTGAAG 1 cut(s) 773
AfaI GTAC 6 cut(s) 58, 235, 404, 435, 593, 680
AfiI CCNNNNNNNGG 3 cut(s) 236, 289, 747
AgsI TTSAA 6 cut(s) 68, 100, 447, 486, 539, 650
AjnI CCWGG 5 cut(s) 134, 229, 297, 396, 900
AlwI GGATC 2 cut(s) 300, 731
AlwNI CAGNNNCTG 1 cut(s) 602
Ama87I CYCGRG 1 cut(s) 417
AoxI GGCC 4 cut(s) 219, 559, 673, 749
ApeKI GCWGC 7 cut(s) 286, 423, 596, 654, 704, 784, 919
ApoI RAATTY 3 cut(s) 317, 407, 819
Asp700I GAANNNNTTC 1 cut(s) 104
Asp718I GGTACC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 673
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 3 cut(s) 182, 287, 322
AvaI CYCGRG 1 cut(s) 417
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 2 cut(s) 36, 396
BbvI GCAGC 7 cut(s) 273, 435, 608, 666, 716, 796, 931
BciT130I CCWGG 5 cut(s) 136, 231, 299, 398, 902
BcnI CCSGG 1 cut(s) 38
BfaI CTAG 3 cut(s) 60, 264, 377
BfmI CTRYAG 2 cut(s) 287, 841
BglII AGATCT 1 cut(s) 102
BisI GCNGC 8 cut(s) 287, 424, 597, 655, 705, 785, 917, 920
BlpI GCTNAGC 1 cut(s) 708
BlsI GCNGC 8 cut(s) 288, 425, 598, 656, 706, 786, 918, 921
BmcAI AGTACT 2 cut(s) 58, 680
Bme1390I CCNGG 6 cut(s) 38, 136, 231, 299, 398, 902
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 673
BmiI GGNNCC 4 cut(s) 35, 235, 281, 395
BmrFI CCNGG 6 cut(s) 38, 136, 231, 299, 398, 902
BmsI GCATC 1 cut(s) 249
Bpu1102I GCTNAGC 1 cut(s) 708
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 328, 741
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 289, 747
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 2 cut(s) 676, 798
Bse3DI GCAATG 2 cut(s) 246, 884
BseBI CCWGG 5 cut(s) 136, 231, 299, 398, 902
BseDI CCNNGG 2 cut(s) 328, 741
BseGI GGATG 3 cut(s) 200, 343, 532
BseLI CCNNNNNNNGG 3 cut(s) 236, 289, 747
BseMI GCAATG 2 cut(s) 246, 884
BseMII CTCAG 1 cut(s) 594
BseNI ACTGG 2 cut(s) 676, 798
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 7 cut(s) 273, 435, 608, 666, 716, 796, 931
BshFI GGCC 4 cut(s) 221, 561, 675, 751
BshNI GGYRCC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 3 cut(s) 38, 237, 283
BsiWI CGTACG 1 cut(s) 591
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 3 cut(s) 236, 289, 747
BsmFI GGGAC 1 cut(s) 480
BsmI GAATGC 2 cut(s) 208, 578
BsnI GGCC 4 cut(s) 221, 561, 675, 751
BsoBI CYCGRG 1 cut(s) 417
Bsp1286I GDGCHC 2 cut(s) 36, 396
Bsp143I GATC 3 cut(s) 102, 292, 736
Bsp1720I GCTNAGC 1 cut(s) 708
BspACI CCGC 2 cut(s) 325, 916
BspANI GGCC 4 cut(s) 221, 561, 675, 751
BspCNI CTCAG 1 cut(s) 595
BspLI GGNNCC 4 cut(s) 35, 235, 281, 395
BspMAI CTGCAG 2 cut(s) 291, 845
BspPI GGATC 2 cut(s) 300, 731
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 2 cut(s) 246, 884
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 2 cut(s) 676, 798
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 328, 741
BssMI GATC 3 cut(s) 102, 292, 736
BssT1I CCWWGG 2 cut(s) 328, 741
Bst2UI CCWGG 5 cut(s) 136, 231, 299, 398, 902
Bst4CI ACNGT 1 cut(s) 184
BstAPI GCANNNNNTGC 1 cut(s) 888
BstDEI CTNAG 3 cut(s) 554, 603, 708
BstF5I GGATG 3 cut(s) 200, 343, 532
BstKTI GATC 3 cut(s) 105, 295, 739
BstMBI GATC 3 cut(s) 102, 292, 736
BstMWI GCNNNNNNNGC 2 cut(s) 701, 888
BstNI CCWGG 5 cut(s) 136, 231, 299, 398, 902
BstSCI CCNGG 6 cut(s) 36, 134, 229, 297, 396, 900
BstSFI CTRYAG 2 cut(s) 287, 841
BstV1I GCAGC 7 cut(s) 273, 435, 608, 666, 716, 796, 931
BstX2I RGATCY 2 cut(s) 102, 736
BstXI CCANNNNNNTGG 1 cut(s) 805
BstYI RGATCY 2 cut(s) 102, 736
BsuRI GGCC 4 cut(s) 221, 561, 675, 751
BtgZI GCGATG 1 cut(s) 672
BtsCI GGATG 3 cut(s) 200, 343, 532
BtsIMutI CAGTG 2 cut(s) 567, 805
CaiI CAGNNNCTG 1 cut(s) 602
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 673
Csp6I GTAC 6 cut(s) 57, 234, 403, 434, 592, 679
CviAII CATG 4 cut(s) 563, 757, 805, 924
CviQI GTAC 6 cut(s) 57, 234, 403, 434, 592, 679
DdeI CTNAG 3 cut(s) 554, 603, 708
DpnI GATC 3 cut(s) 104, 294, 738
DpnII GATC 3 cut(s) 102, 292, 736
DraI TTTAAA 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 328, 741
Eco24I GRGCYC 2 cut(s) 36, 396
Eco57I CTGAAG 1 cut(s) 773
Eco88I CYCGRG 1 cut(s) 417
EcoRII CCWGG 5 cut(s) 134, 229, 297, 396, 900
EcoT14I CCWWGG 2 cut(s) 328, 741
EcoT22I ATGCAT 1 cut(s) 929
EcoT38I GRGCYC 2 cut(s) 36, 396
ErhI CCWWGG 2 cut(s) 328, 741
FaeI CATG 4 cut(s) 566, 760, 808, 927
FaqI GGGAC 1 cut(s) 480
FatI CATG 4 cut(s) 562, 756, 804, 923
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 8 cut(s) 287, 424, 597, 655, 705, 785, 917, 920
FokI GGATG 3 cut(s) 207, 330, 539
FriOI GRGCYC 2 cut(s) 36, 396
Fsp4HI GCNGC 8 cut(s) 287, 424, 597, 655, 705, 785, 917, 920
FspBI CTAG 3 cut(s) 60, 264, 377
GluI GCNGC 8 cut(s) 287, 424, 597, 655, 705, 785, 917, 920
HaeIII GGCC 4 cut(s) 221, 561, 675, 751
HapII CCGG 3 cut(s) 38, 237, 283
Hin1II CATG 4 cut(s) 566, 760, 808, 927
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 4 cut(s) 173, 482, 584, 794
HpaII CCGG 3 cut(s) 38, 237, 283
HphI GGTGA 3 cut(s) 182, 287, 322
Hpy166II GTNNAC 3 cut(s) 11, 187, 403
Hpy188I TCNGA 1 cut(s) 871
Hpy188III TCNNGA 5 cut(s) 377, 479, 581, 602, 791
Hpy8I GTNNAC 3 cut(s) 11, 187, 403
HpyAV CCTTC 5 cut(s) 62, 533, 543, 741, 846
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 5 cut(s) 85, 289, 843, 882, 927
HpyF10VI GCNNNNNNNGC 2 cut(s) 701, 888
HpyF3I CTNAG 3 cut(s) 554, 603, 708
Hsp92II CATG 4 cut(s) 566, 760, 808, 927
KpnI GGTACC 1 cut(s) 237
Kzo9I GATC 3 cut(s) 102, 292, 736
LmnI GCTCC 6 cut(s) 39, 74, 399, 604, 692, 781
Lsp1109I GCAGC 7 cut(s) 273, 435, 608, 666, 716, 796, 931
LweI GCATC 1 cut(s) 249
MaeI CTAG 3 cut(s) 60, 264, 377
MaeIII GTNAC 2 cut(s) 310, 800
MalI GATC 3 cut(s) 104, 294, 738
MboI GATC 3 cut(s) 102, 292, 736
MboII GAAGA 4 cut(s) 97, 112, 467, 839
MflI RGATCY 2 cut(s) 102, 736
MhlI GDGCHC 2 cut(s) 36, 396
MluCI AATT 4 cut(s) 63, 317, 407, 819
MnlI CCTC 9 cut(s) 270, 373, 413, 426, 517, 551, 620, 802, 849
Mph1103I ATGCAT 1 cut(s) 929
MroXI GAANNNNTTC 1 cut(s) 104
MseI TTAA 3 cut(s) 42, 150, 321
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 3 cut(s) 38, 237, 283
MspR9I CCNGG 6 cut(s) 38, 136, 231, 299, 398, 902
Mva1269I GAATGC 2 cut(s) 208, 578
MvaI CCWGG 5 cut(s) 136, 231, 299, 398, 902
MwoI GCNNNNNNNGC 2 cut(s) 701, 888
NciI CCSGG 1 cut(s) 38
NdeII GATC 3 cut(s) 102, 292, 736
NlaIII CATG 4 cut(s) 566, 760, 808, 927
NlaIV GGNNCC 4 cut(s) 35, 235, 281, 395
NmuCI GTSAC 2 cut(s) 310, 800
NsiI ATGCAT 1 cut(s) 929
PaeR7I CTCGAG 1 cut(s) 417
PctI GAATGC 2 cut(s) 208, 578
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 4 cut(s) 173, 482, 584, 794
Pfl23II CGTACG 1 cut(s) 591
PkrI GCNGC 8 cut(s) 288, 425, 598, 656, 706, 786, 918, 921
Psp6I CCWGG 5 cut(s) 134, 229, 297, 396, 900
PspGI CCWGG 5 cut(s) 134, 229, 297, 396, 900
PspLI CGTACG 1 cut(s) 591
PspN4I GGNNCC 4 cut(s) 35, 235, 281, 395
PspPI GGNCC 1 cut(s) 673
PspXI VCTCGAGB 1 cut(s) 417
PstI CTGCAG 2 cut(s) 291, 845
PstNI CAGNNNCTG 1 cut(s) 602
PsuI RGATCY 2 cut(s) 102, 736
RsaI GTAC 6 cut(s) 58, 235, 404, 435, 593, 680
RsaNI GTAC 6 cut(s) 57, 234, 403, 434, 592, 679
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 3 cut(s) 42, 150, 321
SatI GCNGC 8 cut(s) 287, 424, 597, 655, 705, 785, 917, 920
Sau3AI GATC 3 cut(s) 102, 292, 736
Sau96I GGNCC 1 cut(s) 673
ScaI AGTACT 2 cut(s) 58, 680
ScrFI CCNGG 6 cut(s) 38, 136, 231, 299, 398, 902
SduI GDGCHC 2 cut(s) 36, 396
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 2 cut(s) 287, 841
Sfr274I CTCGAG 1 cut(s) 417
SlaI CTCGAG 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 2 cut(s) 215, 417
SmoI CTYRAG 2 cut(s) 215, 417
Sse9I AATT 4 cut(s) 63, 317, 407, 819
SsiI CCGC 2 cut(s) 325, 916
SspI AATATT 1 cut(s) 157
SspMI CTAG 3 cut(s) 60, 264, 377
StyD4I CCNGG 6 cut(s) 36, 134, 229, 297, 396, 900
StyI CCWWGG 2 cut(s) 328, 741
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 418
TasI AATT 4 cut(s) 63, 317, 407, 819
TatI WGTACW 3 cut(s) 56, 433, 678
TauI GCSGC 1 cut(s) 919
TfiI GAWTC 4 cut(s) 173, 482, 584, 794
Tru1I TTAA 3 cut(s) 42, 150, 321
Tru9I TTAA 3 cut(s) 42, 150, 321
TscAI CASTG 2 cut(s) 574, 805
TseFI GTSAC 2 cut(s) 310, 800
TseI GCWGC 7 cut(s) 286, 423, 596, 654, 704, 784, 919
Tsp45I GTSAC 2 cut(s) 310, 800
TspDTI ATGAA 7 cut(s) 132, 332, 507, 576, 681, 773, 846
TspGWI ACGGA 2 cut(s) 134, 629
TspRI CASTG 2 cut(s) 574, 805
XapI RAATTY 3 cut(s) 317, 407, 819
XbaI TCTAGA 1 cut(s) 376
XhoI CTCGAG 1 cut(s) 417
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 3 cut(s) 60, 264, 377
ZrmI AGTACT 2 cut(s) 58, 680
Zsp2I ATGCAT 1 cut(s) 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.