Rorug01G0379300

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013746
Physical Location & Seq
Reverse (-)
49235378 .. 49236277
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0379300.1

Sequence Viewer

Length: 900 bp
ATGAGGGCCAACGCCGTTGCAAGAGCCACAAAACCTCCTCGTCCTCGTCGGAGAGCAGACCTCTGTTACAATTATCCCAGCTTTCGGCATTCTCATTATCAAACACCAGTTCTTGCAGACAACACTTCATTTTCAAAGGACAATGAAGATGATGATCCATTGCCAGGTATAGACTGCCTCCAAATTCCAGGTGAAGCTCTTCCGGGATCCGAACTTCAAGCATGTGGATACCTAACTAAGGGAGCAACCAGTTGCAACTTTCAGTGGGTGCGCCATCATCCGGATGGATCATTTCATTACATTAATGGAGAAACAAAACCAAAATATCTCATTACTGCTGACGATGTTGATGCATACCTTGCTATTGAAGTTGAGCCTCTAGATGATCAAAGGAACAGAAAGGGAGAGCTTGAAAAAGTCTTTGCCAATAAGCACAACAAGATCAGTTTTGGCCCTAAAATGCAATACCAGATACACAAAATACTTTTCGAGGGTGAGAAATCACTTCGAGTGTTTCATTTCACTCGATGTCTAAATAAATGGCAATCTGCTATGTTGGTCATTAAGAGGAGATCTTACCGAGTTGAAGACCTTGTCTTTGGTGGAGTTAGTGTTATACTCCCTGACCAAAAGTATTCGCTGCACACAGTTGTTTCGATTCCTTATGGAAGTCCTGTAGAATTCATGATAACTGATTTTGATAGTAGCTGCGGTGAAGTTGGTGATCATCTATTCAAATTAATGGACACATCAATTTATTGGTCTAGAGATGTAGTTGTGCTCATACTGAGATCATTTATCCAAAGGGTTGTTCAAAAAAGCTCAAGCGAAGGAAAAGGAAAGCTGGAGGAGAAGGAGAAAACAAAAGATTTTTCTTTCTTTCCTGAAAATCCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

299

Amino Acids

34.2

Weight (kDa)

7.65

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IG_AIR9 PF23197 60 - 129 9e-07 AIR9 A9 domain
DUF7046 PF23080 173 - 275 1.4e-08 Domain of unknown function (DUF7046)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 280
AciI CCGC 1 cut(s) 711
AclWI GGATC 4 cut(s) 149, 201, 214, 295
AcsI RAATTY 2 cut(s) 183, 680
AfiI CCNNNNNNNGG 4 cut(s) 84, 164, 238, 280
AgsI TTSAA 7 cut(s) 135, 218, 368, 413, 587, 736, 815
AjnI CCWGG 2 cut(s) 163, 187
AjuI GAANNNNNNNTTGG 2 cut(s) 795, 827
AluBI AGCT 6 cut(s) 81, 197, 409, 708, 822, 844
AluI AGCT 6 cut(s) 81, 197, 409, 708, 822, 844
Alw21I GWGCWC 1 cut(s) 783
AlwI GGATC 4 cut(s) 149, 201, 214, 295
Aor13HI TCCGGA 1 cut(s) 280
AoxI GGCC 2 cut(s) 6, 451
ApeKI GCWGC 2 cut(s) 640, 708
ApoI RAATTY 2 cut(s) 183, 680
ArsI GACNNNNNNTTYG 2 cut(s) 581, 613
AseI ATTAAT 2 cut(s) 303, 740
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 2 cut(s) 6, 452
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 4 cut(s) 203, 506, 725, 734
BamHI GGATCC 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 783
BbvI GCAGC 2 cut(s) 627, 695
BccI CCATC 2 cut(s) 278, 282
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 2 cut(s) 165, 189
BciVI GTATCC 1 cut(s) 221
BclI TGATCA 2 cut(s) 385, 724
BcnI CCSGG 1 cut(s) 204
BfaI CTAG 2 cut(s) 380, 765
BfmI CTRYAG 1 cut(s) 675
BfuI GTATCC 1 cut(s) 221
BglII AGATCT 1 cut(s) 572
BisI GCNGC 2 cut(s) 641, 709
BlsI GCNGC 2 cut(s) 642, 710
Bme1390I CCNGG 3 cut(s) 165, 189, 204
BmgT120I GGNCC 2 cut(s) 6, 452
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 3 cut(s) 165, 189, 204
BmsI GCATC 1 cut(s) 340
BpiI GAAGAC 1 cut(s) 594
BplI GAGNNNNNCTC 2 cut(s) 45, 77
BpmI CTGGAG 1 cut(s) 866
BpuEI CTTGAG 1 cut(s) 808
BpuMI CCSGG 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 597, 627
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 164, 238, 280
Bse1I ACTGG 2 cut(s) 107, 249
Bse3DI GCAATG 1 cut(s) 158
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 280
BseBI CCWGG 2 cut(s) 165, 189
BseGI GGATG 2 cut(s) 277, 289
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 4 cut(s) 84, 164, 238, 280
BseMI GCAATG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 779
BseNI ACTGG 2 cut(s) 107, 249
BseRI GAGGAG 3 cut(s) 27, 583, 863
BseXI GCAGC 2 cut(s) 627, 695
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 1 cut(s) 626
BshFI GGCC 2 cut(s) 8, 453
BsiHKAI GWGCWC 1 cut(s) 783
BsiSI CCGG 2 cut(s) 203, 281
BslI CCNNNNNNNGG 4 cut(s) 84, 164, 238, 280
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 2 cut(s) 8, 453
Bsp1286I GDGCHC 1 cut(s) 783
Bsp13I TCCGGA 1 cut(s) 280
Bsp143I GATC 8 cut(s) 154, 206, 287, 385, 441, 572, 724, 791
BspACI CCGC 1 cut(s) 711
BspANI GGCC 2 cut(s) 8, 453
BspCNI CTCAG 1 cut(s) 780
BspEI TCCGGA 1 cut(s) 280
BspHI TCATGA 1 cut(s) 684
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 4 cut(s) 149, 201, 214, 295
BspQI GCTCTTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 158
BsrI ACTGG 2 cut(s) 107, 249
BssMI GATC 8 cut(s) 154, 206, 287, 385, 441, 572, 724, 791
Bst2UI CCWGG 2 cut(s) 165, 189
Bst4CI ACNGT 1 cut(s) 649
Bst6I CTCTTC 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 359
BstDEI CTNAG 2 cut(s) 237, 788
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 2 cut(s) 277, 289
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 8 cut(s) 157, 209, 290, 388, 444, 575, 727, 794
BstMBI GATC 8 cut(s) 154, 206, 287, 385, 441, 572, 724, 791
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 2 cut(s) 165, 189
BstNSI RCATGY 1 cut(s) 225
BstSCI CCNGG 3 cut(s) 163, 187, 202
BstSFI CTRYAG 1 cut(s) 675
BstV1I GCAGC 2 cut(s) 627, 695
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 2 cut(s) 206, 572
BstYI RGATCY 2 cut(s) 206, 572
BsuI GTATCC 1 cut(s) 221
BsuRI GGCC 2 cut(s) 8, 453
BtsCI GGATG 2 cut(s) 277, 289
BtsIMutI CAGTG 1 cut(s) 269
CciI TCATGA 1 cut(s) 684
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 2 cut(s) 6, 452
CviAII CATG 2 cut(s) 222, 685
DdeI CTNAG 2 cut(s) 237, 788
DpnI GATC 8 cut(s) 156, 208, 289, 387, 443, 574, 726, 793
DpnII GATC 8 cut(s) 154, 206, 287, 385, 441, 572, 724, 791
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 163, 187
EcoT22I ATGCAT 1 cut(s) 355
FaeI CATG 2 cut(s) 225, 688
FaiI YATR 8 cut(s) 170, 223, 355, 554, 617, 666, 686, 785
FatI CATG 2 cut(s) 221, 684
FbaI TGATCA 2 cut(s) 385, 724
Fnu4HI GCNGC 2 cut(s) 641, 709
FokI GGATG 2 cut(s) 264, 296
Fsp4HI GCNGC 2 cut(s) 641, 709
FspBI CTAG 2 cut(s) 380, 765
GlaI GCGC 1 cut(s) 272
GluI GCNGC 2 cut(s) 641, 709
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 2 cut(s) 8, 453
HapII CCGG 2 cut(s) 203, 281
HhaI GCGC 1 cut(s) 273
Hin1II CATG 2 cut(s) 225, 688
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HinfI GANTC 1 cut(s) 658
HpaII CCGG 2 cut(s) 203, 281
HphI GGTGA 4 cut(s) 203, 506, 725, 734
Hpy188I TCNGA 2 cut(s) 51, 211
Hpy188III TCNNGA 6 cut(s) 281, 380, 685, 765, 884, 897
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 2 cut(s) 824, 847
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4V TGCA 6 cut(s) 20, 116, 255, 353, 463, 643
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 2 cut(s) 237, 788
Hsp92II CATG 2 cut(s) 225, 688
HspAI GCGC 1 cut(s) 271
Kpn2I TCCGGA 1 cut(s) 280
Ksp22I TGATCA 2 cut(s) 385, 724
Kzo9I GATC 8 cut(s) 154, 206, 287, 385, 441, 572, 724, 791
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 2 cut(s) 627, 695
LweI GCATC 1 cut(s) 340
MaeI CTAG 2 cut(s) 380, 765
MaeIII GTNAC 1 cut(s) 65
MalI GATC 8 cut(s) 156, 208, 289, 387, 443, 574, 726, 793
MboI GATC 8 cut(s) 154, 206, 287, 385, 441, 572, 724, 791
MboII GAAGA 3 cut(s) 158, 191, 599
MflI RGATCY 2 cut(s) 206, 572
MhlI GDGCHC 1 cut(s) 783
MluCI AATT 5 cut(s) 70, 183, 680, 737, 753
MmeI TCCRAC 1 cut(s) 29
MnlI CCTC 9 cut(s) 45, 48, 54, 71, 188, 387, 484, 561, 841
Mph1103I ATGCAT 1 cut(s) 355
MroI TCCGGA 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 3 cut(s) 303, 564, 740
MslI CAYNNNNRTG 1 cut(s) 282
MspI CCGG 2 cut(s) 203, 281
MspR9I CCNGG 3 cut(s) 165, 189, 204
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 2 cut(s) 165, 189
MwoI GCNNNNNNNGC 1 cut(s) 359
NciI CCSGG 1 cut(s) 204
NdeII GATC 8 cut(s) 154, 206, 287, 385, 441, 572, 724, 791
NlaIII CATG 2 cut(s) 225, 688
NlaIV GGNNCC 1 cut(s) 208
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 1 cut(s) 225
PagI TCATGA 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 204
PctI GAATGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 658
PflFI GACNNNGTC 1 cut(s) 593
PfoI TCCNGGA 1 cut(s) 202
PkrI GCNGC 2 cut(s) 642, 710
PshBI ATTAAT 2 cut(s) 303, 740
Psp6I CCWGG 2 cut(s) 163, 187
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 163, 187
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 6, 452
PsuI RGATCY 2 cut(s) 206, 572
PsyI GACNNNGTC 1 cut(s) 593
RseI CAYNNNNRTG 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 3 cut(s) 303, 564, 740
SatI GCNGC 2 cut(s) 641, 709
Sau3AI GATC 8 cut(s) 154, 206, 287, 385, 441, 572, 724, 791
Sau96I GGNCC 2 cut(s) 6, 452
ScrFI CCNGG 3 cut(s) 165, 189, 204
SduI GDGCHC 1 cut(s) 783
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 675
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
Sse9I AATT 5 cut(s) 70, 183, 680, 737, 753
SsiI CCGC 1 cut(s) 711
SspMI CTAG 2 cut(s) 380, 765
StyD4I CCNGG 3 cut(s) 163, 187, 202
TaaI ACNGT 1 cut(s) 649
TaqI TCGA 4 cut(s) 489, 508, 526, 656
TasI AATT 5 cut(s) 70, 183, 680, 737, 753
TfiI GAWTC 1 cut(s) 658
Tru1I TTAA 3 cut(s) 303, 564, 740
Tru9I TTAA 3 cut(s) 303, 564, 740
TscAI CASTG 1 cut(s) 269
TseI GCWGC 2 cut(s) 640, 708
TspDTI ATGAA 5 cut(s) 117, 159, 284, 506, 673
TspRI CASTG 1 cut(s) 269
Tth111I GACNNNGTC 1 cut(s) 593
VspI ATTAAT 2 cut(s) 303, 740
XagI CCTNNNNNAGG 1 cut(s) 236
XapI RAATTY 2 cut(s) 183, 680
XbaI TCTAGA 2 cut(s) 379, 764
XceI RCATGY 1 cut(s) 225
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 2 cut(s) 380, 765
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.