RchiOBHm_Chr1g0376971

Predicted transmembrane protein 161AB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016225
Physical Location & Seq
Reverse (-)
64128600 .. 64130500
1901 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1359 bp
ATGCTGGACCAATTCTTGACCTACAAGAATCTCATACTCCAGCTCTCACTCTCTCTCACCCTCACTCTCCTCCTCACCTTCCTCAAAATCCCCATCTTCTTCCTCCACAGCCTCCACACCTACATCCACCCCGATAACATCCCGCCCGGCGGAACCCGGGCCGCCATCCGCCGACCCGACTCGTCCGATTCTGGACCCGGGTCCAAACCCAACCCGGAGCTCCGAAAGAGAACCAAGTCCAAGTCCAAGAACACCCCGGCCTTCGATGAAAACAACGCCCAGATCTTCCGGCTCAAGCTCGACGAGGCCCACCTCCAGACCCGCCTCTATTTCCCGGACTACTCCAACGCCTTCACCTTCTCCTTCGTCGCCGTCTCTGCCCTCGCGCTCCAAATATATCTAGGTGATTCGCAATCGGATTCTGGAGTTCTGGTCAATGGGGTCTTCGTTCCGGTTCTGCTAGGGTTTACGGGGGTTTCGAAGCTGCTGCTGGTTCTTGCGAAGGTTTCGGCGGAGAAATCGGCGTCGAGGAGGTCGGAGAAGCAGTTGAGTGTGGCGCTGGGGGTTGTGGGGTTGGTGGTGGGGAGTGTAGTGTGCTTTAGATTTAGTCCGTCGGTTTTGGATTTTGATTTCGGGTCGGTTGATGGGAACGGAAGGCTGTTTATTGCAATTTTGATGGGGTGCTTTTCGGGTTTCTTGTTCATGCCTGCGGCGAAGAGTGCGAGGGCGTTTTGGCTCGGGACTGATCAGATTAGGTCCGATTTGTCGATGCTTTCGTGTGAATGGTTTGGGAGAATGGTGCTTTACTTGAATTCTGCTGTGGTTCTGTTTACGGTCTTGTTGTGGATCAATCCGTTTGGTGGAATGCTTGTGAACAAGAATGGGAAAGTTCGAAATGCAGAGGGTTTGATTGGGAATGTAGGGTTTTCTGCTTCGGATTTTGCTAAGTTTCGGCTTTGGTGCTTGCTGGTTTCGAGTTTTCTGCAGCTGGTTGCGTTGCGGCCGAATTTACAAATGTATTTGAATGAGGCATTGTTGTCTTGGTATCAGAGATTGCACGCTGGGAAGGTTCCTGACTTGGAGTTCAGTAGGGCTAAGGTGTTTCTGCACAATCATTACTTATGCCTCGTGGTTCTGCAGTTCCTCGCACCGCCAGTATTGTTGCTTCTGTTTCTTGGGCTGTCTCGGGTTGATGGCATCTCTCCAGAAGATTACCAATATGTTTGTAGTGTGCTACCCTGTTCTGCCTTTGTGAAAGAAGTGGCGGTCTTTATGGCTTGGTGGATTCTCTTTCTTTGGGCCGTTTATAGTTCAGCTACCGTTTTTCTGTATCGTCGCTGCATTTTGTACATGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_161AB

Protein Analysis

452

Amino Acids

50.45

Weight (kDa)

9.31

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_161AB PF10268 219 - 392 6.8e-16 Predicted transmembrane protein 161AB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52180
fragaria_vesca FvH4_7g29210
malus_domestica MD01G1198800.v1.1 MD07G1266600.v1.1
prunus_persica Prupe.2G292200_v2.0.a1
pyrus_communis pycom07g24440
rosa_chinensis RchiOBHm_Chr1g0376971
rosa_laevigata RLG00000026555
rosa_roxburghii Rroxscaffold_4G00281600
rosa_rugosa Rorug01G0400000
rosa_samantha Rh1AG417200 Rh1BG376600 Rh1CG390400 Rh1DG407300
rosa_wichuraiana Rw1G036620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 386
AclWI GGATC 1 cut(s) 854
AcoI YGGCCR 1 cut(s) 1001
AcsI RAATTY 2 cut(s) 811, 1006
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 1 cut(s) 1349
AfiI CCNNNNNNNGG 2 cut(s) 149, 860
AflIII ACRYGT 1 cut(s) 1350
AgsI TTSAA 2 cut(s) 811, 1024
AluBI AGCT 6 cut(s) 43, 220, 298, 484, 988, 1316
AluI AGCT 6 cut(s) 43, 220, 298, 484, 988, 1316
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 379, 1188
AlwI GGATC 1 cut(s) 854
Ama87I CYCGRG 4 cut(s) 156, 197, 737, 1185
AoxI GGCC 5 cut(s) 159, 258, 306, 1001, 1299
ApeKI GCWGC 4 cut(s) 484, 487, 985, 1338
ApoI RAATTY 2 cut(s) 811, 1006
AspLEI GCGC 2 cut(s) 388, 559
AspS9I GGNCC 7 cut(s) 7, 159, 194, 201, 307, 756, 1299
AsuC2I CCSGG 8 cut(s) 147, 157, 158, 198, 199, 215, 257, 335
AsuHPI GGTGA 4 cut(s) 49, 67, 346, 416
AsuII TTCGAA 2 cut(s) 479, 892
AvaI CYCGRG 4 cut(s) 156, 197, 737, 1185
AvaII GGWCC 4 cut(s) 7, 194, 201, 756
BanII GRGCYC 1 cut(s) 222
BarI GAAGNNNNNNTAC 2 cut(s) 1149, 1181
BauI CACGAG 1 cut(s) 1127
BbsI GAAGAC 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 4 cut(s) 471, 474, 997, 1325
BccI CCATC 5 cut(s) 101, 173, 638, 670, 1187
BceAI ACGGC 2 cut(s) 356, 1286
BcgI CGANNNNNNTGC 4 cut(s) 469, 503, 964, 998
BclI TGATCA 1 cut(s) 745
BcnI CCSGG 8 cut(s) 147, 157, 158, 198, 199, 215, 257, 335
BcoDI GTCTC 2 cut(s) 379, 1188
BfaI CTAG 2 cut(s) 401, 461
BfmI CTRYAG 2 cut(s) 983, 1136
BfoI RGCGCY 1 cut(s) 560
BglII AGATCT 1 cut(s) 282
BisI GCNGC 7 cut(s) 162, 485, 488, 711, 986, 1001, 1339
BlsI GCNGC 7 cut(s) 163, 486, 489, 712, 987, 1002, 1340
Bme1390I CCNGG 8 cut(s) 147, 157, 158, 198, 199, 215, 257, 335
Bme18I GGWCC 4 cut(s) 7, 194, 201, 756
BmeT110I CYCGRG 4 cut(s) 156, 197, 737, 1185
BmgT120I GGNCC 7 cut(s) 7, 159, 194, 201, 307, 756, 1299
BmiI GGNNCC 4 cut(s) 154, 196, 202, 1071
BmrFI CCNGG 8 cut(s) 147, 157, 158, 198, 199, 215, 257, 335
BmsI GCATC 2 cut(s) 759, 1206
BoxI GACNNNNGTC 1 cut(s) 199
BpiI GAAGAC 1 cut(s) 436
BpmI CTGGAG 4 cut(s) 23, 299, 444, 1188
Bpu10I CCTNAGC 1 cut(s) 1095
Bpu14I TTCGAA 2 cut(s) 479, 892
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 8 cut(s) 147, 157, 158, 198, 199, 215, 257, 335
BsaHI GRCGYC 1 cut(s) 524
BsaJI CCNNGG 3 cut(s) 156, 197, 255
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 4 cut(s) 417, 447, 530, 560
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 860
Bse1I ACTGG 1 cut(s) 1154
BseDI CCNNGG 3 cut(s) 156, 197, 255
BseGI GGATG 3 cut(s) 123, 138, 165
BseLI CCNNNNNNNGG 2 cut(s) 149, 860
BseNI ACTGG 1 cut(s) 1154
BseRI GAGGAG 3 cut(s) 59, 62, 544
BseX3I CGGCCG 1 cut(s) 1001
BseXI GCAGC 4 cut(s) 471, 474, 997, 1325
BseYI CCCAGC 2 cut(s) 559, 1061
BsgI GTGCAG 1 cut(s) 1091
Bsh1236I CGCG 1 cut(s) 386
Bsh1285I CGRYCG 1 cut(s) 1004
BshFI GGCC 5 cut(s) 161, 260, 308, 1003, 1301
BsiEI CGRYCG 1 cut(s) 1004
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 4 cut(s) 156, 197, 737, 1185
BsiSI CCGG 8 cut(s) 147, 157, 198, 215, 257, 289, 335, 452
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 2 cut(s) 149, 860
BsmAI GTCTC 2 cut(s) 379, 1188
BsmBI CGTCTC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 754
BsmI GAATGC 1 cut(s) 870
BsnI GGCC 5 cut(s) 161, 260, 308, 1003, 1301
BsoBI CYCGRG 4 cut(s) 156, 197, 737, 1185
Bsp119I TTCGAA 2 cut(s) 479, 892
Bsp1286I GDGCHC 1 cut(s) 222
Bsp1407I TGTACA 1 cut(s) 1347
Bsp143I GATC 3 cut(s) 282, 745, 846
BspANI GGCC 5 cut(s) 161, 260, 308, 1003, 1301
BspFNI CGCG 1 cut(s) 386
BspLI GGNNCC 4 cut(s) 154, 196, 202, 1071
BspMAI CTGCAG 2 cut(s) 987, 1140
BspPI GGATC 1 cut(s) 854
BspT104I TTCGAA 2 cut(s) 479, 892
BsrGI TGTACA 1 cut(s) 1347
BsrI ACTGG 1 cut(s) 1154
BssECI CCNNGG 3 cut(s) 156, 197, 255
BssMI GATC 3 cut(s) 282, 745, 846
BssNI GRCGYC 1 cut(s) 524
BssSI CACGAG 1 cut(s) 1127
Bst2BI CACGAG 1 cut(s) 1127
Bst4CI ACNGT 2 cut(s) 835, 1321
Bst6I CTCTTC 1 cut(s) 710
BstACI GRCGYC 1 cut(s) 524
BstAUI TGTACA 1 cut(s) 1347
BstBI TTCGAA 2 cut(s) 479, 892
BstC8I GCNNGC 3 cut(s) 708, 965, 1059
BstDEI CTNAG 2 cut(s) 945, 1095
BstF5I GGATG 3 cut(s) 123, 138, 165
BstFNI CGCG 1 cut(s) 386
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 2 cut(s) 388, 559
BstKTI GATC 3 cut(s) 285, 748, 849
BstMAI GTCTC 2 cut(s) 379, 1188
BstMBI GATC 3 cut(s) 282, 745, 846
BstMCI CGRYCG 1 cut(s) 1004
BstMWI GCNNNNNNNGC 2 cut(s) 377, 719
BstNSI RCATGY 1 cut(s) 1354
BstPAI GACNNNNGTC 1 cut(s) 199
BstSCI CCNGG 8 cut(s) 145, 155, 156, 196, 197, 213, 255, 333
BstSFI CTRYAG 2 cut(s) 983, 1136
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 4 cut(s) 471, 474, 997, 1325
BstV2I GAAGAC 1 cut(s) 436
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BstZI CGGCCG 1 cut(s) 1001
BsuRI GGCC 5 cut(s) 161, 260, 308, 1003, 1301
BtsCI GGATG 3 cut(s) 123, 138, 165
Cac8I GCNNGC 3 cut(s) 708, 965, 1059
CfoI GCGC 2 cut(s) 388, 559
Cfr13I GGNCC 7 cut(s) 7, 159, 194, 201, 307, 756, 1299
Cfr9I CCCGGG 2 cut(s) 156, 197
CseI GACGC 1 cut(s) 513
Csp6I GTAC 1 cut(s) 1348
CviAII CATG 2 cut(s) 703, 1351
CviQI GTAC 1 cut(s) 1348
DdeI CTNAG 2 cut(s) 945, 1095
DpnI GATC 3 cut(s) 284, 747, 848
DpnII GATC 3 cut(s) 282, 745, 846
EaeI YGGCCR 1 cut(s) 1001
EagI CGGCCG 1 cut(s) 1001
Eam1104I CTCTTC 1 cut(s) 710
EarI CTCTTC 1 cut(s) 710
EciI GGCGGA 3 cut(s) 158, 165, 527
Ecl136II GAGCTC 1 cut(s) 220
EclXI CGGCCG 1 cut(s) 1001
Eco24I GRGCYC 1 cut(s) 222
Eco47I GGWCC 4 cut(s) 7, 194, 201, 756
Eco52I CGGCCG 1 cut(s) 1001
Eco53kI GAGCTC 1 cut(s) 220
Eco88I CYCGRG 4 cut(s) 156, 197, 737, 1185
EcoICRI GAGCTC 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 811
EcoT38I GRGCYC 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 379
FaeI CATG 2 cut(s) 706, 1354
FaiI YATR 8 cut(s) 35, 397, 704, 1123, 1221, 1274, 1308, 1352
FaqI GGGAC 1 cut(s) 754
FatI CATG 2 cut(s) 702, 1350
FauI CCCGC 2 cut(s) 150, 329
FbaI TGATCA 1 cut(s) 745
Fnu4HI GCNGC 7 cut(s) 162, 485, 488, 711, 986, 1001, 1339
FokI GGATG 3 cut(s) 110, 125, 152
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 7 cut(s) 162, 485, 488, 711, 986, 1001, 1339
FspBI CTAG 2 cut(s) 401, 461
GlaI GCGC 2 cut(s) 387, 558
GluI GCNGC 7 cut(s) 162, 485, 488, 711, 986, 1001, 1339
GsaI CCCAGC 2 cut(s) 563, 1065
GsuI CTGGAG 4 cut(s) 23, 299, 444, 1188
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 5 cut(s) 161, 260, 308, 1003, 1301
HapII CCGG 8 cut(s) 147, 157, 198, 215, 257, 289, 335, 452
HgaI GACGC 1 cut(s) 513
HhaI GCGC 2 cut(s) 388, 559
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 2 cut(s) 706, 1354
Hin6I GCGC 2 cut(s) 386, 557
HinP1I GCGC 2 cut(s) 386, 557
HinfI GANTC 6 cut(s) 28, 179, 188, 407, 419, 1285
HpaII CCGG 8 cut(s) 147, 157, 198, 215, 257, 289, 335, 452
HphI GGTGA 4 cut(s) 49, 67, 346, 416
Hpy166II GTNNAC 3 cut(s) 468, 831, 874
Hpy188I TCNGA 8 cut(s) 187, 224, 418, 538, 750, 760, 937, 1050
Hpy188III TCNNGA 8 cut(s) 16, 192, 316, 423, 739, 1073, 1205, 1356
Hpy8I GTNNAC 3 cut(s) 468, 831, 874
Hpy99I CGWCG 5 cut(s) 305, 371, 529, 616, 1338
HpyAV CCTTC 8 cut(s) 88, 271, 361, 367, 373, 496, 648, 1060
HpyCH4III ACNGT 2 cut(s) 835, 1321
HpyCH4V TGCA 7 cut(s) 668, 899, 985, 1057, 1108, 1138, 1341
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 719
HpyF3I CTNAG 2 cut(s) 945, 1095
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 2 cut(s) 706, 1354
HspAI GCGC 2 cut(s) 386, 557
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 3 cut(s) 282, 745, 846
LmnI GCTCC 3 cut(s) 217, 225, 393
Lsp1109I GCAGC 4 cut(s) 471, 474, 997, 1325
LweI GCATC 2 cut(s) 759, 1206
MaeI CTAG 2 cut(s) 401, 461
MalI GATC 3 cut(s) 284, 747, 848
MboI GATC 3 cut(s) 282, 745, 846
MboII GAAGA 6 cut(s) 88, 91, 277, 436, 727, 1220
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 4 cut(s) 11, 669, 811, 1006
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 2 cut(s) 369, 516
MspA1I CMGCKG 1 cut(s) 988
MspI CCGG 8 cut(s) 147, 157, 198, 215, 257, 289, 335, 452
MspR9I CCNGG 8 cut(s) 147, 157, 158, 198, 199, 215, 257, 335
Mva1269I GAATGC 1 cut(s) 870
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 377, 719
NciI CCSGG 8 cut(s) 147, 157, 158, 198, 199, 215, 257, 335
NdeII GATC 3 cut(s) 282, 745, 846
NlaIII CATG 2 cut(s) 706, 1354
NlaIV GGNNCC 4 cut(s) 154, 196, 202, 1071
NspI RCATGY 1 cut(s) 1354
NspV TTCGAA 2 cut(s) 479, 892
PciI ACATGT 1 cut(s) 1350
PcsI WCGNNNNNNNCGW 2 cut(s) 476, 773
PctI GAATGC 1 cut(s) 870
PfeI GAWTC 5 cut(s) 28, 188, 407, 419, 1285
PfoI TCCNGGA 1 cut(s) 333
PkrI GCNGC 7 cut(s) 163, 486, 489, 712, 987, 1002, 1340
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PscI ACATGT 1 cut(s) 1350
PshAI GACNNNNGTC 1 cut(s) 199
Psp124BI GAGCTC 1 cut(s) 222
PspFI CCCAGC 2 cut(s) 559, 1061
PspN4I GGNNCC 4 cut(s) 154, 196, 202, 1071
PspPI GGNCC 7 cut(s) 7, 159, 194, 201, 307, 756, 1299
PstI CTGCAG 2 cut(s) 987, 1140
PsuI RGATCY 1 cut(s) 282
PvuII CAGCTG 1 cut(s) 988
RsaI GTAC 1 cut(s) 1349
RsaNI GTAC 1 cut(s) 1348
SacI GAGCTC 1 cut(s) 222
SatI GCNGC 7 cut(s) 162, 485, 488, 711, 986, 1001, 1339
Sau3AI GATC 3 cut(s) 282, 745, 846
Sau96I GGNCC 7 cut(s) 7, 159, 194, 201, 307, 756, 1299
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 8 cut(s) 147, 157, 158, 198, 199, 215, 257, 335
SduI GDGCHC 1 cut(s) 222
SfaNI GCATC 2 cut(s) 759, 1206
SfcI CTRYAG 2 cut(s) 983, 1136
SfuI TTCGAA 2 cut(s) 479, 892
SinI GGWCC 4 cut(s) 7, 194, 201, 756
SmaI CCCGGG 2 cut(s) 158, 199
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 4 cut(s) 11, 669, 811, 1006
SspMI CTAG 2 cut(s) 401, 461
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 8 cut(s) 145, 155, 156, 196, 197, 213, 255, 333
TaaI ACNGT 2 cut(s) 835, 1321
TaqI TCGA 7 cut(s) 264, 300, 479, 527, 767, 892, 974
TasI AATT 4 cut(s) 11, 669, 811, 1006
TatI WGTACW 1 cut(s) 1347
TauI GCSGC 3 cut(s) 164, 713, 1003
TfiI GAWTC 5 cut(s) 28, 188, 407, 419, 1285
TseI GCWGC 4 cut(s) 484, 487, 985, 1338
TspDTI ATGAA 2 cut(s) 282, 691
TspGWI ACGGA 3 cut(s) 600, 666, 843
TspMI CCCGGG 2 cut(s) 156, 197
VpaK11BI GGWCC 4 cut(s) 7, 194, 201, 756
XapI RAATTY 2 cut(s) 811, 1006
XceI RCATGY 1 cut(s) 1354
XmaI CCCGGG 2 cut(s) 156, 197
XspI CTAG 2 cut(s) 401, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.