RchiOBHm_Chr1g0382361

Cytochrome b5-like Heme/Steroid binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004918
Physical Location & Seq
Forward (+)
67291701 .. 67296358
4658 bp
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UTR
Exon/CDS
Intron
PRQ60535

Sequence Viewer

Length: 2025 bp
ATGGGGAACACACACAAGGATGAAAGTGAAACCATGGAGATTGAGAATTTCGAGAGAGAAAATGTGGAGCAAAATGGAGTTGTTGACCTTTTGGAGATGAACAGAATCCCTCCATGGAAGAAACAGATCACAGCTCGGGGACTTGTGGCGAGTGTGGTCATTGGGATCATTTACAGTGTCATAGTGCAGAAGCTGAACCTCACAACTGGTTTGATTCCCAATCTCAATGTCTCAGCTGCTCTTCTGGCCTTTGTGTTTGTGAAGACTTGGACTACGCTGCTATCGAAGGCTGGAATTGTGTCCACTCCTTTCACAAGGCAAGAGAATACCGTGATTCAGACTTGTGCTGTTGCTTGTTATAGCATTGCTGTTGGAGGAGGGTTTGGGTCTTACCTGTTGGGTTTGAACAGGAAGACTTATGAGCTAGCTGGAGTTGATACTGAAGGGAACACTCCAGGGAGCACCAAGGAGCCTCAACTTGGTTGGATGACTGGTTTTCTCTTTGTTACTAGCTTTGTTGGTCTTTTGGCTTTGGTTCCTCTCAGAAAGATTATGATAATAGACTATAAACTAGCGTACCCGAGTGGAACTGCTACTGCTGTTCTTATTAATGGGTTCCATACTCCAAAAGGAGACAAAATGGCCAAGAAGCAGGTTCATGGGTTTATGAAGTACTTTTCATTTAGTTTCTTGTGGGCTGGCTTTCAGTGGTTTTATGCCGGCGGGGAGCAATGTGGATTTGTTCAGTTTCCAACATTTGGATTGAAAGCTTGGAAGAATTCATTCTACTTTGATTTCAGTATGACATATATTGGAGCAGGAATGATCTGTTCCCATCTTGTGAACTTATCTCTGCTTCTTGGGGCCGTGCTCTCTTGGGGAATAATGTGGCCTATATTAAGGGGCCTTAAAGGAGAATGGTTTCCTGCAACTTTATCAGAAAGTAGTATGAAGAGTGTAAACGGTTACAAGGTTTTTATTTCCATTTCCCTGATACTAGGAGATGGTCTGTACAATTTTCTCAAGATACTATACTTTACCGGCTCAAGTATGCACACCAGACTGAACAGGAAGGACCTCACAAAAGATGATATTTCAATTGAGCCGAATCAGCCTCTTGATGATCGCCGACGAAATGAGGTCTTCATAAGAGATAAGATTCCTATTTGGGTAGCATGTGTAGGGTACACACTGTTCTCCATCATCTCCATCATTGTTATCCCTCTCATGTTTCCTCAGCTGAAATGGTATTATGTAGTCGTTGCCTACATTCTTGCACCATCTCTAAGCTTCTGCAATGCCTACGGTGCGGGTTTAACAGACATGAACATGGCCTACAACTATGGGAAAGTAGCTCTTTTCGTGCTAGCTGCTGTGACTGGGAAAAATGATGGTGTTGTTGCAGGACTTGTTGGCTGTGGTTTGATCAAGTCCATAGTTTCTATCTCTTCTGACCTGATGCATGATTTGAAGACGGGCCATCTAACTCTCACATCTCCTCGATCAATGATTGTTAGTCAGGCTATTGGGACAGCTATTGGCTGTGTGGTTGCTCCTCTGACATTCTTTCTCTTCTACAAGGCTTTTGATGTTGGGAACCCAAATGGTCAATACAAAGCCCCTTATGCCATCATTTACCGAAACATGGCAATTCTCGGTGTGGAAGGCTTCTCTGCACTTCCCCAACATTGCTTGCAGATATGCTATGGTTTTTTTGGCTTTGCTGTAGCAGCCAATTTGCTGAGAGATCTCGCTCCTAAGAAAATCGGCAAATATGTCCCCCTTCCAATGGCTATGGCTGTTCCTTTCCTGGTTGGAGCTTACTTTGCAATTGACATGTGCGTAGGGAGTTTGATCGTGTTTGTGTGGCACAAATTGAAACATAATAAGGCCAGTTTGATGGTTCCTGCAGTTGCTTCTGGTTTGATCTGTGGAGATGGATTGTGGATTCTCCCTTCGTCGATTCTTGCATTGGCCAAGCTCGATTTTTTCACGTCCTGGATGAGATCAAGGTGCCTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5 OPT

Protein Analysis

674

Amino Acids

74.16

Weight (kDa)

9.12

Isoelectric Point (pI)

29.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 42 - 656 1e-157 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 643
AccB1I GGYRCC 1 cut(s) 2013
AccB7I CCANNNNNTGG 1 cut(s) 758
AciI CCGC 2 cut(s) 723, 1310
AclWI GGATC 1 cut(s) 173
AcoI YGGCCR 2 cut(s) 642, 1974
AcsI RAATTY 2 cut(s) 46, 778
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 4 cut(s) 578, 674, 1013, 1187
AfiI CCNNNNNNNGG 4 cut(s) 479, 758, 1645, 1789
AflIII ACRYGT 1 cut(s) 1836
AgsI TTSAA 5 cut(s) 406, 766, 1098, 1471, 1879
AjiI CACGTC 1 cut(s) 1995
AjnI CCWGG 4 cut(s) 454, 1809, 1997, 2016
Alw21I GWGCWC 2 cut(s) 464, 873
Alw26I GTCTC 2 cut(s) 235, 627
AlwI GGATC 1 cut(s) 173
AlwNI CAGNNNCTG 1 cut(s) 193
Ama87I CYCGRG 2 cut(s) 135, 580
AoxI GGCC 9 cut(s) 246, 642, 864, 890, 904, 1332, 1477, 1890, 1974
ApeKI GCWGC 4 cut(s) 236, 277, 1370, 1730
ApoI RAATTY 2 cut(s) 46, 778
AseI ATTAAT 1 cut(s) 609
Asp700I GAANNNNTTC 2 cut(s) 782, 921
AspS9I GGNCC 4 cut(s) 864, 904, 1075, 1477
AsuNHI GCTAGC 2 cut(s) 424, 1366
AvaI CYCGRG 2 cut(s) 135, 580
AvaII GGWCC 1 cut(s) 1075
BaeI ACNNNNGTAYC 2 cut(s) 560, 593
BalI TGGCCA 2 cut(s) 644, 1976
BanI GGYRCC 1 cut(s) 2013
BbsI GAAGAC 4 cut(s) 269, 419, 1135, 1478
Bbv12I GWGCWC 2 cut(s) 464, 873
BbvCI CCTCAGC 1 cut(s) 1236
BbvI GCAGC 4 cut(s) 223, 264, 1357, 1742
BceAI ACGGC 1 cut(s) 851
BciT130I CCWGG 4 cut(s) 456, 1811, 1999, 2018
BclI TGATCA 1 cut(s) 1425
BcoDI GTCTC 2 cut(s) 235, 627
BfaI CTAG 5 cut(s) 425, 510, 572, 998, 1367
BfmI CTRYAG 2 cut(s) 1725, 1908
BfuAI ACCTGC 1 cut(s) 643
BglII AGATCT 1 cut(s) 1747
BisI GCNGC 4 cut(s) 237, 278, 1371, 1731
BlsI GCNGC 4 cut(s) 238, 279, 1372, 1732
BmcAI AGTACT 1 cut(s) 674
Bme1390I CCNGG 4 cut(s) 456, 1811, 1999, 2018
Bme18I GGWCC 1 cut(s) 1075
BmeT110I CYCGRG 2 cut(s) 135, 580
BmgBI CACGTC 1 cut(s) 1995
BmgT120I GGNCC 4 cut(s) 864, 904, 1075, 1477
BmiI GGNNCC 8 cut(s) 471, 537, 617, 865, 905, 1598, 1905, 2015
BmrFI CCNGG 4 cut(s) 456, 1811, 1999, 2018
BmrI ACTGGG 1 cut(s) 1389
BmsI GCATC 1 cut(s) 1449
BmtI GCTAGC 2 cut(s) 428, 1370
BmuI ACTGGG 1 cut(s) 1389
BpiI GAAGAC 4 cut(s) 269, 419, 1135, 1478
BpmI CTGGAG 2 cut(s) 438, 450
Bpu10I CCTNAGC 1 cut(s) 1236
BpuEI CTTGAG 2 cut(s) 1007, 1030
BsaJI CCNNGG 4 cut(s) 33, 113, 455, 465
Bsc4I CCNNNNNNNGG 4 cut(s) 479, 758, 1645, 1789
Bse118I RCCGGY 2 cut(s) 719, 1040
Bse1I ACTGG 4 cut(s) 211, 496, 1384, 1893
Bse3DI GCAATG 4 cut(s) 363, 737, 1303, 1687
BseBI CCWGG 4 cut(s) 456, 1811, 1999, 2018
BseDI CCNNGG 4 cut(s) 33, 113, 455, 465
BseGI GGATG 3 cut(s) 25, 492, 2007
BseLI CCNNNNNNNGG 4 cut(s) 479, 758, 1645, 1789
BseMI GCAATG 4 cut(s) 363, 737, 1303, 1687
BseMII CTCAG 4 cut(s) 246, 556, 1250, 1733
BseNI ACTGG 4 cut(s) 211, 496, 1384, 1893
BseRI GAGGAG 3 cut(s) 390, 1488, 1545
BseXI GCAGC 4 cut(s) 223, 264, 1357, 1742
BsgI GTGCAG 2 cut(s) 206, 1659
BshFI GGCC 9 cut(s) 248, 644, 866, 892, 906, 1334, 1479, 1892, 1976
BshNI GGYRCC 1 cut(s) 2013
BsiHKAI GWGCWC 2 cut(s) 464, 873
BsiHKCI CYCGRG 2 cut(s) 135, 580
BsiSI CCGG 2 cut(s) 720, 1041
BslFI GGGAC 3 cut(s) 153, 1543, 1763
BslI CCNNNNNNNGG 4 cut(s) 479, 758, 1645, 1789
BsmAI GTCTC 2 cut(s) 235, 627
BsmFI GGGAC 3 cut(s) 153, 1543, 1763
BsnI GGCC 9 cut(s) 248, 644, 866, 892, 906, 1334, 1479, 1892, 1976
BsoBI CYCGRG 2 cut(s) 135, 580
Bsp1286I GDGCHC 2 cut(s) 464, 873
Bsp1407I TGTACA 1 cut(s) 1011
Bsp19I CCATGG 2 cut(s) 33, 113
BspACI CCGC 2 cut(s) 723, 1310
BspANI GGCC 9 cut(s) 248, 644, 866, 892, 906, 1334, 1479, 1892, 1976
BspCNI CTCAG 4 cut(s) 245, 555, 1249, 1734
BspLI GGNNCC 8 cut(s) 471, 537, 617, 865, 905, 1598, 1905, 2015
BspMAI CTGCAG 1 cut(s) 1912
BspMI ACCTGC 1 cut(s) 643
BspOI GCTAGC 2 cut(s) 428, 1370
BspPI GGATC 1 cut(s) 173
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 2013
BsrDI GCAATG 4 cut(s) 363, 737, 1303, 1687
BsrFI RCCGGY 2 cut(s) 719, 1040
BsrGI TGTACA 1 cut(s) 1011
BsrI ACTGG 4 cut(s) 211, 496, 1384, 1893
BssAI RCCGGY 2 cut(s) 719, 1040
BssECI CCNNGG 4 cut(s) 33, 113, 455, 465
BssT1I CCWWGG 3 cut(s) 33, 113, 465
Bst2UI CCWGG 4 cut(s) 456, 1811, 1999, 2018
Bst4CI ACNGT 5 cut(s) 176, 331, 965, 1194, 1307
Bst6I CTCTTC 4 cut(s) 246, 947, 1453, 1577
BstAUI TGTACA 1 cut(s) 1011
BstC8I GCNNGC 5 cut(s) 426, 700, 721, 1368, 1694
BstDEI CTNAG 6 cut(s) 232, 542, 1236, 1286, 1742, 1758
BstDSI CCRYGG 2 cut(s) 33, 113
BstF5I GGATG 3 cut(s) 25, 492, 2007
BstMAI GTCTC 2 cut(s) 235, 627
BstMWI GCNNNNNNNGC 6 cut(s) 245, 1111, 1307, 1625, 1730, 1826
BstNI CCWGG 4 cut(s) 456, 1811, 1999, 2018
BstNSI RCATGY 2 cut(s) 1179, 1840
BstSCI CCNGG 4 cut(s) 454, 1809, 1997, 2016
BstSFI CTRYAG 2 cut(s) 1725, 1908
BstV1I GCAGC 4 cut(s) 223, 264, 1357, 1742
BstV2I GAAGAC 4 cut(s) 269, 419, 1135, 1478
BstX2I RGATCY 1 cut(s) 1747
BstXI CCANNNNNNTGG 1 cut(s) 1900
BstYI RGATCY 1 cut(s) 1747
BsuRI GGCC 9 cut(s) 248, 644, 866, 892, 906, 1334, 1479, 1892, 1976
BtgI CCRYGG 2 cut(s) 33, 113
BtrI CACGTC 1 cut(s) 1995
BtsCI GGATG 3 cut(s) 25, 492, 2007
BtsIMutI CAGTG 3 cut(s) 181, 713, 1190
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 5 cut(s) 426, 700, 721, 1368, 1694
CaiI CAGNNNCTG 1 cut(s) 193
Cfr10I RCCGGY 2 cut(s) 719, 1040
Cfr13I GGNCC 4 cut(s) 864, 904, 1075, 1477
Csp6I GTAC 4 cut(s) 577, 673, 1012, 1186
CviQI GTAC 4 cut(s) 577, 673, 1012, 1186
DdeI CTNAG 6 cut(s) 232, 542, 1236, 1286, 1742, 1758
EaeI YGGCCR 2 cut(s) 642, 1974
Eam1104I CTCTTC 4 cut(s) 246, 947, 1453, 1577
EarI CTCTTC 4 cut(s) 246, 947, 1453, 1577
Eco130I CCWWGG 3 cut(s) 33, 113, 465
Eco47I GGWCC 1 cut(s) 1075
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 2 cut(s) 135, 580
EcoO109I RGGNCCY 2 cut(s) 904, 1075
EcoRI GAATTC 1 cut(s) 778
EcoRII CCWGG 4 cut(s) 454, 1809, 1997, 2016
EcoT14I CCWWGG 3 cut(s) 33, 113, 465
EcoT22I ATGCAT 1 cut(s) 1464
ErhI CCWWGG 3 cut(s) 33, 113, 465
FalI AAGNNNNNCTT 2 cut(s) 1341, 1373
FaqI GGGAC 3 cut(s) 153, 1543, 1763
FauI CCCGC 2 cut(s) 716, 1303
FbaI TGATCA 1 cut(s) 1425
Fnu4HI GCNGC 4 cut(s) 237, 278, 1371, 1731
FokI GGATG 3 cut(s) 32, 499, 2014
Fsp4HI GCNGC 4 cut(s) 237, 278, 1371, 1731
FspBI CTAG 5 cut(s) 425, 510, 572, 998, 1367
GluI GCNGC 4 cut(s) 237, 278, 1371, 1731
GsuI CTGGAG 2 cut(s) 438, 450
HaeIII GGCC 9 cut(s) 248, 644, 866, 892, 906, 1334, 1479, 1892, 1976
HapII CCGG 2 cut(s) 720, 1041
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HindIII AAGCTT 2 cut(s) 768, 1288
HinfI GANTC 7 cut(s) 105, 214, 334, 1108, 1159, 1948, 1963
HpaII CCGG 2 cut(s) 720, 1041
Hpy166II GTNNAC 5 cut(s) 85, 303, 844, 961, 1188
Hpy188I TCNGA 5 cut(s) 339, 545, 940, 1453, 1560
Hpy188III TCNNGA 3 cut(s) 52, 1024, 1118
Hpy8I GTNNAC 5 cut(s) 85, 303, 844, 961, 1188
Hpy99I CGWCG 2 cut(s) 1134, 1963
HpyAV CCTTC 6 cut(s) 280, 437, 1066, 1658, 1793, 1965
HpyCH4III ACNGT 5 cut(s) 176, 331, 965, 1194, 1307
HpyCH4IV ACGT 1 cut(s) 1994
HpyF10VI GCNNNNNNNGC 6 cut(s) 245, 1111, 1307, 1625, 1730, 1826
HpyF3I CTNAG 6 cut(s) 232, 542, 1236, 1286, 1742, 1758
HpySE526I ACGT 1 cut(s) 1994
KroI GCCGGC 1 cut(s) 719
KroNI GCCGGC 1 cut(s) 721
Ksp22I TGATCA 1 cut(s) 1425
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 8 cut(s) 67, 459, 469, 727, 815, 1558, 1759, 1817
Lsp1109I GCAGC 4 cut(s) 223, 264, 1357, 1742
LweI GCATC 1 cut(s) 1449
MaeI CTAG 5 cut(s) 425, 510, 572, 998, 1367
MaeII ACGT 1 cut(s) 1994
MaeIII GTNAC 3 cut(s) 505, 965, 1375
MfeI CAATTG 2 cut(s) 1098, 1830
MflI RGATCY 1 cut(s) 1747
MhlI GDGCHC 2 cut(s) 464, 873
MlsI TGGCCA 2 cut(s) 644, 1976
MluCI AATT 9 cut(s) 46, 294, 778, 1015, 1098, 1650, 1735, 1830, 1874
MluNI TGGCCA 2 cut(s) 644, 1976
MmeI TCCRAC 4 cut(s) 352, 464, 776, 1795
Mox20I TGGCCA 2 cut(s) 644, 1976
Mph1103I ATGCAT 1 cut(s) 1464
MroNI GCCGGC 1 cut(s) 719
MroXI GAANNNNTTC 2 cut(s) 782, 921
MscI TGGCCA 2 cut(s) 644, 1976
MseI TTAA 5 cut(s) 609, 899, 909, 1316, 2023
MslI CAYNNNNRTG 2 cut(s) 18, 1328
Msp20I TGGCCA 2 cut(s) 644, 1976
MspA1I CMGCKG 2 cut(s) 236, 1240
MspI CCGG 2 cut(s) 720, 1041
MspR9I CCNGG 4 cut(s) 456, 1811, 1999, 2018
MunI CAATTG 2 cut(s) 1098, 1830
MvaI CCWGG 4 cut(s) 456, 1811, 1999, 2018
MwoI GCNNNNNNNGC 6 cut(s) 245, 1111, 1307, 1625, 1730, 1826
NaeI GCCGGC 1 cut(s) 721
NcoI CCATGG 2 cut(s) 33, 113
NgoMIV GCCGGC 1 cut(s) 719
NheI GCTAGC 2 cut(s) 424, 1366
NlaIV GGNNCC 8 cut(s) 471, 537, 617, 865, 905, 1598, 1905, 2015
NmuCI GTSAC 1 cut(s) 1375
NsiI ATGCAT 1 cut(s) 1464
NspI RCATGY 2 cut(s) 1179, 1840
PciI ACATGT 1 cut(s) 1836
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 281
PdiI GCCGGC 1 cut(s) 721
PdmI GAANNNNTTC 2 cut(s) 782, 921
PfeI GAWTC 7 cut(s) 105, 214, 334, 1108, 1159, 1948, 1963
PflMI CCANNNNNTGG 1 cut(s) 758
PfoI TCCNGGA 1 cut(s) 1997
PkrI GCNGC 4 cut(s) 238, 279, 1372, 1732
PpuMI RGGWCCY 1 cut(s) 1075
PscI ACATGT 1 cut(s) 1836
PshBI ATTAAT 1 cut(s) 609
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 4 cut(s) 454, 1809, 1997, 2016
PspGI CCWGG 4 cut(s) 454, 1809, 1997, 2016
PspN4I GGNNCC 8 cut(s) 471, 537, 617, 865, 905, 1598, 1905, 2015
PspPI GGNCC 4 cut(s) 864, 904, 1075, 1477
PspPPI RGGWCCY 1 cut(s) 1075
PsrI GAACNNNNNNTAC 4 cut(s) 1178, 1210, 1319, 1351
PstI CTGCAG 1 cut(s) 1912
PstNI CAGNNNCTG 1 cut(s) 193
PsuI RGATCY 1 cut(s) 1747
PvuII CAGCTG 2 cut(s) 236, 1240
RsaI GTAC 4 cut(s) 578, 674, 1013, 1187
RsaNI GTAC 4 cut(s) 577, 673, 1012, 1186
RseI CAYNNNNRTG 2 cut(s) 18, 1328
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 5 cut(s) 609, 899, 909, 1316, 2023
SatI GCNGC 4 cut(s) 237, 278, 1371, 1731
Sau96I GGNCC 4 cut(s) 864, 904, 1075, 1477
ScaI AGTACT 1 cut(s) 674
ScrFI CCNGG 4 cut(s) 456, 1811, 1999, 2018
SduI GDGCHC 2 cut(s) 464, 873
SfaNI GCATC 1 cut(s) 1449
SfcI CTRYAG 2 cut(s) 1725, 1908
SinI GGWCC 1 cut(s) 1075
SmiMI CAYNNNNRTG 2 cut(s) 18, 1328
SmlI CTYRAG 2 cut(s) 1022, 1045
SmoI CTYRAG 2 cut(s) 1022, 1045
Sse9I AATT 9 cut(s) 46, 294, 778, 1015, 1098, 1650, 1735, 1830, 1874
SsiI CCGC 2 cut(s) 723, 1310
SspMI CTAG 5 cut(s) 425, 510, 572, 998, 1367
StyD4I CCNGG 4 cut(s) 454, 1809, 1997, 2016
StyI CCWWGG 3 cut(s) 33, 113, 465
TaaI ACNGT 5 cut(s) 176, 331, 965, 1194, 1307
TaiI ACGT 1 cut(s) 1997
TaqI TCGA 5 cut(s) 51, 284, 1501, 1961, 1983
TasI AATT 9 cut(s) 46, 294, 778, 1015, 1098, 1650, 1735, 1830, 1874
TatI WGTACW 2 cut(s) 672, 1011
TfiI GAWTC 7 cut(s) 105, 214, 334, 1108, 1159, 1948, 1963
Tru1I TTAA 5 cut(s) 609, 899, 909, 1316, 2023
Tru9I TTAA 5 cut(s) 609, 899, 909, 1316, 2023
TscAI CASTG 3 cut(s) 181, 713, 1197
TseFI GTSAC 1 cut(s) 1375
TseI GCWGC 4 cut(s) 236, 277, 1370, 1730
Tsp45I GTSAC 1 cut(s) 1375
TspDTI ATGAA 9 cut(s) 36, 113, 647, 669, 683, 771, 965, 1135, 1340
TspRI CASTG 3 cut(s) 181, 713, 1197
Van91I CCANNNNNTGG 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 1075
VspI ATTAAT 1 cut(s) 609
XapI RAATTY 2 cut(s) 46, 778
XceI RCATGY 2 cut(s) 1179, 1840
XmnI GAANNNNTTC 2 cut(s) 782, 921
XspI CTAG 5 cut(s) 425, 510, 572, 998, 1367
ZrmI AGTACT 1 cut(s) 674
Zsp2I ATGCAT 1 cut(s) 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.