Rh1AG458400

Cytochrome b5-like Heme/Steroid binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004918
Physical Location & Seq
Forward (+)
67702727 .. 67707558
4832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG458400.1

Sequence Viewer

Length: 2019 bp
ATGGGGAACACACGCAAGGATGAAACCATGGAGATTGAGAATTTCGAGAGAGAAAATGTGGAGCAAAATGGAGTTGTTGACCCTTTGGAGATGAACAGAATCCCTCCATGGAAGAAACAGATCACAATTCGGGGACTTGTGGCGAGTGTGGTCATTGGGATCATTTACAGTGTCATAGTGCAGAAGCTGAACCTCACAACTGGTTTGATTCCCAATCTCAATGTCTCAGCTGCTCTTCTGGCCTTTGTGTTTGTGAAGACTTGGACTACGCTACTATCGAAGGCTGGAATTGTGTCCACTCCTTTCACAAGGCAAGAGAATACCGTGATTCAGACTTGTGCTGTTGCTTGTTATAGCATTGCTGTTGGAGGAGGGTTTGGGTCTTACCTGTTGGGTTTGAACAGGAAGACTTATGAGCTAGCTGGAGTTGATACTGAAGGGAACACTCCAGGGAGCACCAAGGAACCTCAACTTGGTTGGATGACTGGTTTTCTCTTTGTTACTAGCTTTGTTGGTCTTTTGGCTTTGGTTCCTCTCAGGAAGATTATGATAATAGACTATAAACTAGCTTACCCGAGTGGAACTGCTACTGCTGTTCTTATTAATGGGTTCCATACTCCAAAAGGAGACAAAATGGCCAAGAAGCAGGTTCATGGGTTTATGAAGTACTTTTCATTTAGTTTCTTGTGGGCTGGCTTTCAGTGGTTTTATGCCGGTGGGGAGCAATGTGGGTTTGTTCAGTTTCCAACATTTGGATTGAAAGCTTGGAAGAATTCATTCTACTTTGATTTCAGTATGACATATATTGGAGCAGGGATGATCTGTTCCCATCTTGTGAACTTATCTCTGCTTCTTGGGGCCGTGCTCTCTTGGGGAATAATGTGGCCCATATTAAGGGGCCTTAAAGGAGAATGGTTTCCTGCAACTTTATCAGAAAGTAGTATGAAGAGTGTAAACGGTTACAAGGTTTTTATTTCCATTTCCCTGATACTAGGAGATGGGCTGTACAATTTTCTCAAGATACTATACTTTACCGGCTCAAGTATGCACACCAGACTGAACAGGAAGGACCTCACAAAAGATGATATTTCAATTGACCCGAATCAGCCTCTTGATGATCGCCGACGAAATGAGGTCTTCATAAGAGATAAGATTCCTATTTGGGTAGCATGTGTAGGGTACACACTGTTCTCCATCATCTCCATTGTTGTTATCCCTCTCATGTTTCCTCAGCTGAAATGGTATTATGTAGTCGTTGCCTACATTCTTGCACCGTCTCTAAGCTTCTGCAATGCCTACGGTGCGGGTTTAACAGACATGAACATGGCCTACAACTATGGGAAAGTAGCTCTTTTCGTGCTAGCTGCTGTGACTGGGAAAAATGATGGTGTTGTTGCAGGGCTTGTTGGCTGTGGTTTGATCAAGTCCATAGTTTCTATCTCTTCTGACCTGATGCATGATTTGAAGACGGGCCATCTAACTCTCACATCTCCTCGATCAATGATTGTTAGTCAGGCTATTGGGACAGCTATTGGCTGTGTGGTAGCTCCTCTGACATTCTTTCTCTTCTACAAGGCTTTTGATGTTGGGAACCCAAATGGTCAATACAAAGCCCCTTACGCCATCATTTACCGAAACATGGCAATTCTCGGTGTGGAAGGCTTCTCTGCACTTCCCCAACATTGCTTGCAGATATGCTATGGTTTTTTCGGCTTTGCTGTAGCAGCCAATTTGCTGAGAGATCTCGCTCCTAAGAAAATCGGCAAATATGTCCCCCTTCCAATGGCTATGGCTGTTCCTTTCCTGGTTGGAGCTTACTTTGCAATTGACATGTGCGTAGGGAGTTTGATCGTGTTTGTGTGGCACAAATTGAAACATAATAATGCCAGTTTGATGGTTCCTGCAGTTGCTTCTGGTTTGATCTGTGGAGATGGATTGTGGATTCTCCCTTCATCGATTCTTGCATTGGCCAAGCTCGATTTTTTCACGTTGTGGATGAGATCAAGGTGCCTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5 OPT

Protein Analysis

672

Amino Acids

73.98

Weight (kDa)

9.17

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 40 - 654 7.6e-158 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 2007
AccB7I CCANNNNNTGG 1 cut(s) 752
AciI CCGC 1 cut(s) 1304
AclWI GGATC 1 cut(s) 167
AcoI YGGCCR 2 cut(s) 636, 1968
AcsI RAATTY 2 cut(s) 40, 772
AcuI CTGAAG 1 cut(s) 456
AdeI CACNNNGTG 1 cut(s) 1992
AfaI GTAC 3 cut(s) 668, 1007, 1181
AfiI CCNNNNNNNGG 5 cut(s) 473, 752, 894, 1639, 1783
AflIII ACRYGT 1 cut(s) 1830
AgsI TTSAA 5 cut(s) 400, 760, 1092, 1465, 1873
AjnI CCWGG 3 cut(s) 448, 1803, 2010
AjuI GAANNNNNNNTTGG 2 cut(s) 456, 488
AloI GAACNNNNNNTCC 2 cut(s) 808, 840
Alw21I GWGCWC 2 cut(s) 458, 867
Alw26I GTCTC 3 cut(s) 229, 621, 1281
AlwI GGATC 1 cut(s) 167
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 8 cut(s) 240, 636, 858, 884, 898, 1326, 1471, 1968
ApeKI GCWGC 3 cut(s) 230, 1364, 1724
ApoI RAATTY 2 cut(s) 40, 772
AseI ATTAAT 1 cut(s) 603
Asp700I GAANNNNTTC 2 cut(s) 776, 915
AspS9I GGNCC 5 cut(s) 858, 885, 898, 1069, 1471
AsuNHI GCTAGC 2 cut(s) 418, 1360
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 1 cut(s) 1069
BalI TGGCCA 2 cut(s) 638, 1970
BanI GGYRCC 1 cut(s) 2007
BbsI GAAGAC 4 cut(s) 263, 413, 1129, 1472
Bbv12I GWGCWC 2 cut(s) 458, 867
BbvCI CCTCAGC 1 cut(s) 1230
BbvI GCAGC 3 cut(s) 217, 1351, 1736
BccI CCATC 8 cut(s) 837, 992, 1202, 1379, 1482, 1631, 1888, 1925
BceAI ACGGC 1 cut(s) 845
BciT130I CCWGG 3 cut(s) 450, 1805, 2012
BclI TGATCA 1 cut(s) 1419
BcoDI GTCTC 3 cut(s) 229, 621, 1281
BfaI CTAG 5 cut(s) 419, 504, 566, 992, 1361
BfmI CTRYAG 2 cut(s) 1719, 1902
BfuAI ACCTGC 1 cut(s) 637
BglII AGATCT 1 cut(s) 1741
BisI GCNGC 3 cut(s) 231, 1365, 1725
BlsI GCNGC 3 cut(s) 232, 1366, 1726
BmcAI AGTACT 1 cut(s) 668
Bme1390I CCNGG 3 cut(s) 450, 1805, 2012
Bme18I GGWCC 1 cut(s) 1069
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 5 cut(s) 858, 885, 898, 1069, 1471
BmiI GGNNCC 8 cut(s) 465, 531, 611, 859, 899, 1592, 1899, 2009
BmrFI CCNGG 3 cut(s) 450, 1805, 2012
BmrI ACTGGG 1 cut(s) 1383
BmsI GCATC 1 cut(s) 1443
BmtI GCTAGC 2 cut(s) 422, 1364
BmuI ACTGGG 1 cut(s) 1383
BpiI GAAGAC 4 cut(s) 263, 413, 1129, 1472
BpmI CTGGAG 2 cut(s) 432, 444
Bpu10I CCTNAGC 1 cut(s) 1230
BpuEI CTTGAG 2 cut(s) 1001, 1024
Bsa29I ATCGAT 1 cut(s) 1955
BsaJI CCNNGG 4 cut(s) 27, 107, 449, 459
Bsc4I CCNNNNNNNGG 5 cut(s) 473, 752, 894, 1639, 1783
Bse118I RCCGGY 2 cut(s) 713, 1034
Bse1I ACTGG 4 cut(s) 205, 490, 1378, 1887
Bse3DI GCAATG 4 cut(s) 357, 731, 1297, 1681
BseBI CCWGG 3 cut(s) 450, 1805, 2012
BseCI ATCGAT 1 cut(s) 1955
BseDI CCNNGG 4 cut(s) 27, 107, 449, 459
BseGI GGATG 4 cut(s) 25, 486, 822, 2001
BseLI CCNNNNNNNGG 5 cut(s) 473, 752, 894, 1639, 1783
BseMI GCAATG 4 cut(s) 357, 731, 1297, 1681
BseMII CTCAG 4 cut(s) 240, 550, 1244, 1727
BseNI ACTGG 4 cut(s) 205, 490, 1378, 1887
BseRI GAGGAG 3 cut(s) 384, 1482, 1539
BseXI GCAGC 3 cut(s) 217, 1351, 1736
BsgI GTGCAG 2 cut(s) 200, 1653
BshFI GGCC 8 cut(s) 242, 638, 860, 886, 900, 1328, 1473, 1970
BshNI GGYRCC 1 cut(s) 2007
BshVI ATCGAT 1 cut(s) 1955
BsiHKAI GWGCWC 2 cut(s) 458, 867
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 2 cut(s) 714, 1035
BslFI GGGAC 3 cut(s) 147, 1537, 1757
BslI CCNNNNNNNGG 5 cut(s) 473, 752, 894, 1639, 1783
BsmAI GTCTC 3 cut(s) 229, 621, 1281
BsmBI CGTCTC 1 cut(s) 1281
BsmFI GGGAC 3 cut(s) 147, 1537, 1757
BsnI GGCC 8 cut(s) 242, 638, 860, 886, 900, 1328, 1473, 1970
BsoBI CYCGRG 1 cut(s) 574
Bsp1286I GDGCHC 2 cut(s) 458, 867
Bsp1407I TGTACA 1 cut(s) 1005
Bsp19I CCATGG 2 cut(s) 27, 107
BspACI CCGC 1 cut(s) 1304
BspANI GGCC 8 cut(s) 242, 638, 860, 886, 900, 1328, 1473, 1970
BspCNI CTCAG 4 cut(s) 239, 549, 1243, 1728
BspDI ATCGAT 1 cut(s) 1955
BspLI GGNNCC 8 cut(s) 465, 531, 611, 859, 899, 1592, 1899, 2009
BspMAI CTGCAG 1 cut(s) 1906
BspMI ACCTGC 1 cut(s) 637
BspOI GCTAGC 2 cut(s) 422, 1364
BspPI GGATC 1 cut(s) 167
BspQI GCTCTTC 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 2007
BsrDI GCAATG 4 cut(s) 357, 731, 1297, 1681
BsrFI RCCGGY 2 cut(s) 713, 1034
BsrGI TGTACA 1 cut(s) 1005
BsrI ACTGG 4 cut(s) 205, 490, 1378, 1887
BssAI RCCGGY 2 cut(s) 713, 1034
BssECI CCNNGG 4 cut(s) 27, 107, 449, 459
BssT1I CCWWGG 3 cut(s) 27, 107, 459
Bst2UI CCWGG 3 cut(s) 450, 1805, 2012
Bst4CI ACNGT 6 cut(s) 170, 325, 959, 1188, 1275, 1301
Bst6I CTCTTC 4 cut(s) 240, 941, 1447, 1571
BstAUI TGTACA 1 cut(s) 1005
BstC8I GCNNGC 4 cut(s) 420, 694, 1362, 1688
BstDEI CTNAG 6 cut(s) 226, 536, 1230, 1280, 1736, 1752
BstDSI CCRYGG 2 cut(s) 27, 107
BstF5I GGATG 4 cut(s) 25, 486, 822, 2001
BstMAI GTCTC 3 cut(s) 229, 621, 1281
BstMWI GCNNNNNNNGC 5 cut(s) 239, 1301, 1619, 1724, 1820
BstNI CCWGG 3 cut(s) 450, 1805, 2012
BstNSI RCATGY 2 cut(s) 1173, 1834
BstSCI CCNGG 3 cut(s) 448, 1803, 2010
BstSFI CTRYAG 2 cut(s) 1719, 1902
BstV1I GCAGC 3 cut(s) 217, 1351, 1736
BstV2I GAAGAC 4 cut(s) 263, 413, 1129, 1472
BstX2I RGATCY 1 cut(s) 1741
BstXI CCANNNNNNTGG 1 cut(s) 1894
BstYI RGATCY 1 cut(s) 1741
Bsu15I ATCGAT 1 cut(s) 1955
BsuRI GGCC 8 cut(s) 242, 638, 860, 886, 900, 1328, 1473, 1970
BsuTUI ATCGAT 1 cut(s) 1955
BtgI CCRYGG 2 cut(s) 27, 107
BtsCI GGATG 4 cut(s) 25, 486, 822, 2001
BtsIMutI CAGTG 3 cut(s) 175, 707, 1184
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 4 cut(s) 420, 694, 1362, 1688
CaiI CAGNNNCTG 1 cut(s) 187
Cfr10I RCCGGY 2 cut(s) 713, 1034
Cfr13I GGNCC 5 cut(s) 858, 885, 898, 1069, 1471
ClaI ATCGAT 1 cut(s) 1955
Csp6I GTAC 3 cut(s) 667, 1006, 1180
CviQI GTAC 3 cut(s) 667, 1006, 1180
DdeI CTNAG 6 cut(s) 226, 536, 1230, 1280, 1736, 1752
DraIII CACNNNGTG 1 cut(s) 1992
EaeI YGGCCR 2 cut(s) 636, 1968
Eam1104I CTCTTC 4 cut(s) 240, 941, 1447, 1571
EarI CTCTTC 4 cut(s) 240, 941, 1447, 1571
Eco130I CCWWGG 3 cut(s) 27, 107, 459
Eco47I GGWCC 1 cut(s) 1069
Eco57I CTGAAG 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 574
EcoO109I RGGNCCY 2 cut(s) 898, 1069
EcoRI GAATTC 1 cut(s) 772
EcoRII CCWGG 3 cut(s) 448, 1803, 2010
EcoT14I CCWWGG 3 cut(s) 27, 107, 459
EcoT22I ATGCAT 1 cut(s) 1458
ErhI CCWWGG 3 cut(s) 27, 107, 459
Esp3I CGTCTC 1 cut(s) 1281
FalI AAGNNNNNCTT 2 cut(s) 1335, 1367
FaqI GGGAC 3 cut(s) 147, 1537, 1757
FauI CCCGC 1 cut(s) 1297
FbaI TGATCA 1 cut(s) 1419
Fnu4HI GCNGC 3 cut(s) 231, 1365, 1725
FokI GGATG 4 cut(s) 32, 493, 829, 2008
Fsp4HI GCNGC 3 cut(s) 231, 1365, 1725
FspBI CTAG 5 cut(s) 419, 504, 566, 992, 1361
GluI GCNGC 3 cut(s) 231, 1365, 1725
GsuI CTGGAG 2 cut(s) 432, 444
HaeIII GGCC 8 cut(s) 242, 638, 860, 886, 900, 1328, 1473, 1970
HapII CCGG 2 cut(s) 714, 1035
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 2 cut(s) 762, 1282
HinfI GANTC 7 cut(s) 99, 208, 328, 1102, 1153, 1942, 1957
HpaII CCGG 2 cut(s) 714, 1035
Hpy166II GTNNAC 5 cut(s) 79, 297, 838, 955, 1182
Hpy188I TCNGA 4 cut(s) 333, 934, 1447, 1554
Hpy188III TCNNGA 4 cut(s) 46, 538, 1018, 1112
Hpy8I GTNNAC 5 cut(s) 79, 297, 838, 955, 1182
Hpy99I CGWCG 1 cut(s) 1128
HpyAV CCTTC 6 cut(s) 274, 431, 1060, 1652, 1787, 1959
HpyCH4III ACNGT 6 cut(s) 170, 325, 959, 1188, 1275, 1301
HpyCH4IV ACGT 1 cut(s) 1988
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 1301, 1619, 1724, 1820
HpyF3I CTNAG 6 cut(s) 226, 536, 1230, 1280, 1736, 1752
HpySE526I ACGT 1 cut(s) 1988
Ksp22I TGATCA 1 cut(s) 1419
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 7 cut(s) 61, 453, 721, 809, 1552, 1753, 1811
Lsp1109I GCAGC 3 cut(s) 217, 1351, 1736
LweI GCATC 1 cut(s) 1443
MaeI CTAG 5 cut(s) 419, 504, 566, 992, 1361
MaeII ACGT 1 cut(s) 1988
MaeIII GTNAC 3 cut(s) 499, 959, 1369
MfeI CAATTG 2 cut(s) 1092, 1824
MflI RGATCY 1 cut(s) 1741
MhlI GDGCHC 2 cut(s) 458, 867
MlsI TGGCCA 2 cut(s) 638, 1970
MluNI TGGCCA 2 cut(s) 638, 1970
MmeI TCCRAC 4 cut(s) 346, 458, 770, 1789
Mox20I TGGCCA 2 cut(s) 638, 1970
Mph1103I ATGCAT 1 cut(s) 1458
MroXI GAANNNNTTC 2 cut(s) 776, 915
MscI TGGCCA 2 cut(s) 638, 1970
MseI TTAA 5 cut(s) 603, 893, 903, 1310, 2017
MslI CAYNNNNRTG 2 cut(s) 1322, 1881
Msp20I TGGCCA 2 cut(s) 638, 1970
MspA1I CMGCKG 2 cut(s) 230, 1234
MspI CCGG 2 cut(s) 714, 1035
MspR9I CCNGG 3 cut(s) 450, 1805, 2012
MunI CAATTG 2 cut(s) 1092, 1824
MvaI CCWGG 3 cut(s) 450, 1805, 2012
MwoI GCNNNNNNNGC 5 cut(s) 239, 1301, 1619, 1724, 1820
NcoI CCATGG 2 cut(s) 27, 107
NheI GCTAGC 2 cut(s) 418, 1360
NlaIV GGNNCC 8 cut(s) 465, 531, 611, 859, 899, 1592, 1899, 2009
NmuCI GTSAC 1 cut(s) 1369
NsiI ATGCAT 1 cut(s) 1458
NspI RCATGY 2 cut(s) 1173, 1834
PciI ACATGT 1 cut(s) 1830
PciSI GCTCTTC 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PdmI GAANNNNTTC 2 cut(s) 776, 915
PfeI GAWTC 7 cut(s) 99, 208, 328, 1102, 1153, 1942, 1957
PflMI CCANNNNNTGG 1 cut(s) 752
PkrI GCNGC 3 cut(s) 232, 1366, 1726
PpuMI RGGWCCY 1 cut(s) 1069
PscI ACATGT 1 cut(s) 1830
PshBI ATTAAT 1 cut(s) 603
Psp5II RGGWCCY 1 cut(s) 1069
Psp6I CCWGG 3 cut(s) 448, 1803, 2010
PspGI CCWGG 3 cut(s) 448, 1803, 2010
PspN4I GGNNCC 8 cut(s) 465, 531, 611, 859, 899, 1592, 1899, 2009
PspPI GGNCC 5 cut(s) 858, 885, 898, 1069, 1471
PspPPI RGGWCCY 1 cut(s) 1069
PsrI GAACNNNNNNTAC 4 cut(s) 1172, 1204, 1313, 1345
PstI CTGCAG 1 cut(s) 1906
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 1741
PvuII CAGCTG 2 cut(s) 230, 1234
RsaI GTAC 3 cut(s) 668, 1007, 1181
RsaNI GTAC 3 cut(s) 667, 1006, 1180
RseI CAYNNNNRTG 2 cut(s) 1322, 1881
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 5 cut(s) 603, 893, 903, 1310, 2017
SatI GCNGC 3 cut(s) 231, 1365, 1725
Sau96I GGNCC 5 cut(s) 858, 885, 898, 1069, 1471
ScaI AGTACT 1 cut(s) 668
ScrFI CCNGG 3 cut(s) 450, 1805, 2012
SduI GDGCHC 2 cut(s) 458, 867
SfaNI GCATC 1 cut(s) 1443
SfcI CTRYAG 2 cut(s) 1719, 1902
SinI GGWCC 1 cut(s) 1069
SmiMI CAYNNNNRTG 2 cut(s) 1322, 1881
SmlI CTYRAG 2 cut(s) 1016, 1039
SmoI CTYRAG 2 cut(s) 1016, 1039
SsiI CCGC 1 cut(s) 1304
SspMI CTAG 5 cut(s) 419, 504, 566, 992, 1361
StyD4I CCNGG 3 cut(s) 448, 1803, 2010
StyI CCWWGG 3 cut(s) 27, 107, 459
TaaI ACNGT 6 cut(s) 170, 325, 959, 1188, 1275, 1301
TaiI ACGT 1 cut(s) 1991
TaqI TCGA 5 cut(s) 45, 278, 1495, 1955, 1977
TatI WGTACW 2 cut(s) 666, 1005
TfiI GAWTC 7 cut(s) 99, 208, 328, 1102, 1153, 1942, 1957
Tru1I TTAA 5 cut(s) 603, 893, 903, 1310, 2017
Tru9I TTAA 5 cut(s) 603, 893, 903, 1310, 2017
TscAI CASTG 3 cut(s) 175, 707, 1191
TseFI GTSAC 1 cut(s) 1369
TseI GCWGC 3 cut(s) 230, 1364, 1724
Tsp45I GTSAC 1 cut(s) 1369
TspRI CASTG 3 cut(s) 175, 707, 1191
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 1 cut(s) 1069
VspI ATTAAT 1 cut(s) 603
XapI RAATTY 2 cut(s) 40, 772
XceI RCATGY 2 cut(s) 1173, 1834
XmnI GAANNNNTTC 2 cut(s) 776, 915
XspI CTAG 5 cut(s) 419, 504, 566, 992, 1361
ZrmI AGTACT 1 cut(s) 668
Zsp2I ATGCAT 1 cut(s) 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.