Rorug01G0433800

Cytochrome b5-like Heme/Steroid binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004918
Physical Location & Seq
Reverse (-)
53237707 .. 53240642
2936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0433800.1

Sequence Viewer

Length: 903 bp
ATGTTTCCTTTACACCAGGGAAATAAGCTGGTCTTCCAGATTTCATCATCTAATCCTCACCACCAGAATTCATCAGAAGATAGGATTCTCGGATATGCTTCGCCGGATGGATGCAGCAGTACTGTCCTTAGCAACAACAATATCATGGGGAAAAGCCGGCGGCGGAAATTAGTGGTTGCAGCTTTGGATCAGACAATTATTGAAAATTCAAAGGATAACAAGAAGAAGAAAATGATGATTCATAGGGAGAATGAAAGGCAAAGAAGGCAAGAAATGGCTACCTTATACTCCTGTCTTAGATCCCTACTCCCCCTTGAATTCATCAAGGGAAAGCGTTCGACGTCTGATCACATGAACGAAGCTGTGAACTACATAAAACACCTCCAGAATAGGATCAAAACATTAGACGCAAAGAGAGATGAATTGAAGAAATTCCCTAAAATAAGCACTGTTATTGACCGTCGCTGCACTACTGGAAGCTCTTTGAATTCAGACAAGGTGAATTTGCCTAGCTGTTTTACAGTCCAGCCAAGTTCTGGTGGAGTGCAAATTTTGGTGAGTGGCGGCTTCAGAAAGGATGGGTTACCACTCATTTCAATCTCAAGCGTTTTGGAAATTCTGCTTGGACAAGGGCTTACTGTTGTTAGTTGTGTCTCCTCCCAAGTAAATGAAAGACTCCTTCACACTTTCCAGATTGAGGTCGATGATGTGGAAAGTCTGGATCTTTCTGCGCTGGAACAGAAGCTAGCTAAAGCGCTTCCAACATCCAGAAGTACTATCTCCAAGTTCGAACCCCGAAGCTGTCAAAGTGGCCAGACACTGTGCTGCAATCCACAGTTGGAGCATTTCACATGCGCCGAAGGGGTTTATCTTGGGTCTAGGAAGTCTTTGGGTTCCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5 OPT

Protein Analysis

300

Amino Acids

33.35

Weight (kDa)

9.35

Isoelectric Point (pI)

61.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 81 - 129 8e-12 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 344
AccB7I CCANNNNNTGG 1 cut(s) 536
AciI CCGC 3 cut(s) 160, 163, 564
AclWI GGATC 4 cut(s) 195, 294, 401, 729
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 8 cut(s) 67, 205, 317, 431, 487, 502, 549, 615
AcuI CTGAAG 1 cut(s) 553
AcyI GRCGYC 1 cut(s) 341
AfaI GTAC 2 cut(s) 121, 775
AfeI AGCGCT 1 cut(s) 756
AfiI CCNNNNNNNGG 2 cut(s) 536, 697
AgsI TTSAA 6 cut(s) 203, 210, 317, 427, 487, 597
AjnI CCWGG 1 cut(s) 15
AjuI GAANNNNNNNTTGG 2 cut(s) 606, 638
AluBI AGCT 8 cut(s) 28, 182, 362, 480, 513, 745, 749, 801
AluI AGCT 8 cut(s) 28, 182, 362, 480, 513, 745, 749, 801
Alw26I GTCTC 1 cut(s) 658
AlwI GGATC 4 cut(s) 195, 294, 401, 729
AlwNI CAGNNNCTG 1 cut(s) 820
Aor51HI AGCGCT 1 cut(s) 756
AoxI GGCC 1 cut(s) 811
ApeKI GCWGC 4 cut(s) 114, 179, 465, 825
ApoI RAATTY 8 cut(s) 67, 205, 317, 431, 487, 502, 549, 615
Asp700I GAANNNNTTC 2 cut(s) 334, 431
AspLEI GCGC 3 cut(s) 733, 757, 857
AsuHPI GGTGA 3 cut(s) 50, 511, 568
AsuII TTCGAA 1 cut(s) 789
AsuNHI GCTAGC 1 cut(s) 745
BalI TGGCCA 1 cut(s) 813
BbsI GAAGAC 1 cut(s) 25
BbvI GCAGC 4 cut(s) 126, 191, 452, 812
BccI CCATC 2 cut(s) 101, 572
BciT130I CCWGG 1 cut(s) 17
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 658
BfaI CTAG 3 cut(s) 510, 746, 879
BfoI RGCGCY 1 cut(s) 758
BisI GCNGC 6 cut(s) 115, 161, 180, 466, 565, 826
BlsI GCNGC 6 cut(s) 116, 162, 181, 467, 566, 827
BmcAI AGTACT 2 cut(s) 121, 775
Bme1390I CCNGG 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 895
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 1 cut(s) 101
BmtI GCTAGC 1 cut(s) 749
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 128
Bpu14I TTCGAA 1 cut(s) 789
BpuEI CTTGAG 1 cut(s) 586
BsaHI GRCGYC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 16
Bsc4I CCNNNNNNNGG 2 cut(s) 536, 697
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 478
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 16
BseGI GGATG 4 cut(s) 112, 116, 583, 764
BseLI CCNNNNNNNGG 2 cut(s) 536, 697
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 646
BseXI GCAGC 4 cut(s) 126, 191, 452, 812
BsgI GTGCAG 1 cut(s) 451
BshFI GGCC 1 cut(s) 813
BsiSI CCGG 2 cut(s) 104, 157
BslI CCNNNNNNNGG 2 cut(s) 536, 697
BsmAI GTCTC 1 cut(s) 658
BsnI GGCC 1 cut(s) 813
Bsp119I TTCGAA 1 cut(s) 789
Bsp143I GATC 5 cut(s) 187, 299, 346, 393, 721
BspACI CCGC 3 cut(s) 160, 163, 564
BspANI GGCC 1 cut(s) 813
BspLI GGNNCC 1 cut(s) 895
BspOI GCTAGC 1 cut(s) 749
BspPI GGATC 4 cut(s) 195, 294, 401, 729
BspT104I TTCGAA 1 cut(s) 789
BsrFI RCCGGY 1 cut(s) 156
BsrI ACTGG 1 cut(s) 478
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 1 cut(s) 16
BssMI GATC 5 cut(s) 187, 299, 346, 393, 721
BssNI GRCGYC 1 cut(s) 341
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 7 cut(s) 124, 451, 461, 523, 640, 822, 837
BstACI GRCGYC 1 cut(s) 341
BstBI TTCGAA 1 cut(s) 789
BstC8I GCNNGC 2 cut(s) 158, 747
BstDEI CTNAG 2 cut(s) 128, 296
BstEII GGTNACC 1 cut(s) 582
BstF5I GGATG 4 cut(s) 112, 116, 583, 764
BstH2I RGCGCY 1 cut(s) 758
BstHHI GCGC 3 cut(s) 733, 757, 857
BstKTI GATC 5 cut(s) 190, 302, 349, 396, 724
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 5 cut(s) 187, 299, 346, 393, 721
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 855
BstPI GGTNACC 1 cut(s) 582
BstSCI CCNGG 1 cut(s) 15
BstV1I GCAGC 4 cut(s) 126, 191, 452, 812
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 299, 721
BstYI RGATCY 2 cut(s) 299, 721
BsuRI GGCC 1 cut(s) 813
BtsCI GGATG 4 cut(s) 112, 116, 583, 764
BtsIMutI CAGTG 2 cut(s) 447, 818
Cac8I GCNNGC 2 cut(s) 158, 747
CaiI CAGNNNCTG 1 cut(s) 820
CfoI GCGC 3 cut(s) 733, 757, 857
Cfr10I RCCGGY 1 cut(s) 156
CseI GACGC 1 cut(s) 416
Csp6I GTAC 2 cut(s) 120, 774
CviAII CATG 3 cut(s) 145, 352, 852
CviQI GTAC 2 cut(s) 120, 774
DdeI CTNAG 2 cut(s) 128, 296
DpnI GATC 5 cut(s) 189, 301, 348, 395, 723
DpnII GATC 5 cut(s) 187, 299, 346, 393, 721
EaeI YGGCCR 1 cut(s) 811
EciI GGCGGA 1 cut(s) 178
Eco47III AGCGCT 1 cut(s) 756
Eco57I CTGAAG 1 cut(s) 553
Eco91I GGTNACC 1 cut(s) 582
EcoO65I GGTNACC 1 cut(s) 582
EcoRI GAATTC 3 cut(s) 67, 317, 487
EcoRII CCWGG 1 cut(s) 15
FaeI CATG 3 cut(s) 148, 355, 855
FaiI YATR 7 cut(s) 96, 146, 243, 286, 353, 374, 853
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FatI CATG 3 cut(s) 144, 351, 851
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 6 cut(s) 115, 161, 180, 466, 565, 826
FokI GGATG 4 cut(s) 119, 123, 590, 751
Fsp4HI GCNGC 6 cut(s) 115, 161, 180, 466, 565, 826
FspBI CTAG 3 cut(s) 510, 746, 879
GlaI GCGC 3 cut(s) 732, 756, 856
GluI GCNGC 6 cut(s) 115, 161, 180, 466, 565, 826
GsuI CTGGAG 1 cut(s) 368
HaeII RGCGCY 1 cut(s) 758
HaeIII GGCC 1 cut(s) 813
HapII CCGG 2 cut(s) 104, 157
HgaI GACGC 1 cut(s) 416
HhaI GCGC 3 cut(s) 733, 757, 857
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 3 cut(s) 148, 355, 855
Hin6I GCGC 3 cut(s) 731, 755, 855
HinP1I GCGC 3 cut(s) 731, 755, 855
HinfI GANTC 3 cut(s) 85, 238, 675
HpaII CCGG 2 cut(s) 104, 157
HphI GGTGA 3 cut(s) 50, 511, 568
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 6 cut(s) 76, 92, 192, 346, 493, 572
Hpy188III TCNNGA 5 cut(s) 37, 385, 691, 719, 768
Hpy8I GTNNAC 1 cut(s) 367
Hpy99I CGWCG 2 cut(s) 343, 465
HpyAV CCTTC 3 cut(s) 258, 689, 854
HpyCH4III ACNGT 7 cut(s) 124, 451, 461, 523, 640, 822, 837
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 5 cut(s) 114, 179, 468, 547, 828
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
HpyF3I CTNAG 2 cut(s) 128, 296
HpySE526I ACGT 1 cut(s) 341
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 3 cut(s) 148, 355, 855
HspAI GCGC 3 cut(s) 731, 755, 855
KroI GCCGGC 1 cut(s) 156
KroNI GCCGGC 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 5 cut(s) 187, 299, 346, 393, 721
LmnI GCTCC 1 cut(s) 841
Lsp1109I GCAGC 4 cut(s) 126, 191, 452, 812
LweI GCATC 1 cut(s) 101
MaeI CTAG 3 cut(s) 510, 746, 879
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 1 cut(s) 582
MalI GATC 5 cut(s) 189, 301, 348, 395, 723
MboI GATC 5 cut(s) 187, 299, 346, 393, 721
MboII GAAGA 5 cut(s) 25, 89, 235, 238, 439
MflI RGATCY 2 cut(s) 299, 721
MlsI TGGCCA 1 cut(s) 813
MluNI TGGCCA 1 cut(s) 813
MlyI GAGTC 1 cut(s) 669
MmeI TCCRAC 2 cut(s) 785, 819
MnlI CCTC 4 cut(s) 66, 392, 667, 691
Mox20I TGGCCA 1 cut(s) 813
MroNI GCCGGC 1 cut(s) 156
MroXI GAANNNNTTC 2 cut(s) 334, 431
MscI TGGCCA 1 cut(s) 813
Msp20I TGGCCA 1 cut(s) 813
MspI CCGG 2 cut(s) 104, 157
MspR9I CCNGG 1 cut(s) 17
MvaI CCWGG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 265
NaeI GCCGGC 1 cut(s) 158
NdeII GATC 5 cut(s) 187, 299, 346, 393, 721
NgoMIV GCCGGC 1 cut(s) 156
NheI GCTAGC 1 cut(s) 745
NlaIII CATG 3 cut(s) 148, 355, 855
NlaIV GGNNCC 1 cut(s) 895
NspI RCATGY 1 cut(s) 855
NspV TTCGAA 1 cut(s) 789
PdiI GCCGGC 1 cut(s) 158
PdmI GAANNNNTTC 2 cut(s) 334, 431
PfeI GAWTC 2 cut(s) 85, 238
PflMI CCANNNNNTGG 1 cut(s) 536
PkrI GCNGC 6 cut(s) 116, 162, 181, 467, 566, 827
PleI GAGTC 1 cut(s) 669
PpsI GAGTC 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 15
PspEI GGTNACC 1 cut(s) 582
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 895
PstNI CAGNNNCTG 1 cut(s) 820
PsuI RGATCY 2 cut(s) 299, 721
RsaI GTAC 2 cut(s) 121, 775
RsaNI GTAC 2 cut(s) 120, 774
SatI GCNGC 6 cut(s) 115, 161, 180, 466, 565, 826
Sau3AI GATC 5 cut(s) 187, 299, 346, 393, 721
ScaI AGTACT 2 cut(s) 121, 775
SchI GAGTC 1 cut(s) 669
ScrFI CCNGG 1 cut(s) 17
SfaNI GCATC 1 cut(s) 101
SfuI TTCGAA 1 cut(s) 789
SmlI CTYRAG 1 cut(s) 601
SmoI CTYRAG 1 cut(s) 601
SsiI CCGC 3 cut(s) 160, 163, 564
SspMI CTAG 3 cut(s) 510, 746, 879
StyD4I CCNGG 1 cut(s) 15
TaaI ACNGT 7 cut(s) 124, 451, 461, 523, 640, 822, 837
TaiI ACGT 1 cut(s) 344
TaqI TCGA 3 cut(s) 338, 702, 789
TatI WGTACW 2 cut(s) 119, 773
TauI GCSGC 2 cut(s) 163, 567
TfiI GAWTC 2 cut(s) 85, 238
TscAI CASTG 2 cut(s) 454, 825
TseI GCWGC 4 cut(s) 114, 179, 465, 825
TspDTI ATGAA 8 cut(s) 33, 60, 230, 267, 310, 368, 435, 684
TspRI CASTG 2 cut(s) 454, 825
Van91I CCANNNNNTGG 1 cut(s) 536
XapI RAATTY 8 cut(s) 67, 205, 317, 431, 487, 502, 549, 615
XceI RCATGY 1 cut(s) 855
XcmI CCANNNNNNNNNTGG 1 cut(s) 533
XmnI GAANNNNTTC 2 cut(s) 334, 431
XspI CTAG 3 cut(s) 510, 746, 879
ZraI GACGTC 1 cut(s) 342
ZrmI AGTACT 2 cut(s) 121, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.