Rorug01G0433600

Cytochrome b5-like Heme/Steroid binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004918
Physical Location & Seq
Forward (+)
53228429 .. 53231876
3448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0433600.1

Sequence Viewer

Length: 1092 bp
ATGTCCACAACCATCTCCATCCGCGACTCCGCCCCCACCGCCGCTATGGCCGCAGCCATATCCTCCTCCGACATGAATGGCGGATTCTACTCCCCAGCCAAAGCCAAAGCCAAAGCCACCAGACCCCTCTTCAGCCCACGCTTCCAGCCATTTCGGCGTCTCCGCCACCTCTTTTGCCCGCGCAAGCTCAGACTCGTCGCCTCTGTTTTCCTTCTCTGCTTCGTCGTTTTCCTCTCCTCCAGGCTCAGCTCTCTCATGGGCTGGCTCCCTCCCCAACCCAACTCCTCATTTCCAAGGGGTGGTGGATACACTGTTCTGATAAACACATGGAAGCAAAGCTCTGCTTTGAAGCAATCGGTTGCTCACTATGCCACGTGTGATGGGGTTGCTTCTATTCATGTGGTGTGGTCTGATAGTGATCCGCCCTCGGAAACTTTGAAGTCTCGTCTTGAAAAGATGGTGTTTTCAAAGTCAAGAGCAGCTCAAAAACCTGACTTTAGGTTTGGGATGAGGCAGGATGAGGATTTGAACAACAGGTTCAAGCCGATTGAGGGGATGACAACTGATGCTGTGTTTTCGGTTGATGATGATGTGATTGTCTCGTGCGACACATTGGATTTTGCTTTCGGAGTTTGGCAGTCTGCGCCGAATGGTATGGTTGGGTTCACTCCTAGAATGCATTGGATTGATGAGGAGAAGAGTGGCGTGGCGAATTATGAGTATGGGGGATGGTGGTCAGTTTGGTGGATGGGGACATATAGCATGCTGCTTCCGAAAGCTGCATTCTTTCATAGGAAATACTTGGATTTGTATACTTACGATATGGCCTCATCCATTCGTGATTATGTCACCAGGGAAAGAAACTGTGAAGATATTGCTATGTCATTGCTTGTTGCTAATGCCACCGGTGCACCTCCGATTTGGGTGAAAGGGAAGATGTACGAGCTTGGATCTTCAGGAATTAGCGGTCTGAAAAGGCACAGCAAGAGTCGCAACAAGTGCCTCAATGATCTCATCTCCCTCTTCGGAAGAATTCCCCTTGTACAAACTAATGTTAAAGCTGTCGATGCCAGGTTTGAATGGTTCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5 OPT

Protein Analysis

363

Amino Acids

40.88

Weight (kDa)

9.43

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_64 PF09258 103 - 354 2.8e-68 Glycosyl transferase family 64 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 299
AccI GTMKAC 1 cut(s) 812
AccII CGCG 2 cut(s) 24, 181
AclWI GGATC 2 cut(s) 413, 958
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 1032
AcuI CTGAAG 2 cut(s) 115, 939
AcvI CACGTG 1 cut(s) 375
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 2 cut(s) 941, 1044
AfiI CCNNNNNNNGG 2 cut(s) 299, 551
AflIII ACRYGT 1 cut(s) 374
AgeI ACCGGT 1 cut(s) 905
AgsI TTSAA 7 cut(s) 349, 439, 452, 468, 529, 541, 1079
AjnI CCWGG 3 cut(s) 239, 851, 1070
AloI GAACNNNNNNTCC 2 cut(s) 297, 329
AluBI AGCT 7 cut(s) 187, 249, 339, 482, 779, 946, 1061
AluI AGCT 7 cut(s) 187, 249, 339, 482, 779, 946, 1061
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 3 cut(s) 164, 447, 604
Alw44I GTGCAC 1 cut(s) 909
AlwI GGATC 2 cut(s) 413, 958
AoxI GGCC 2 cut(s) 48, 825
ApaLI GTGCAC 1 cut(s) 909
ApeKI GCWGC 4 cut(s) 53, 479, 766, 779
ApoI RAATTY 1 cut(s) 1032
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AsiGI ACCGGT 1 cut(s) 905
AspLEI GCGC 2 cut(s) 183, 646
AsuHPI GGTGA 2 cut(s) 841, 937
BaeGI GKGCMC 1 cut(s) 913
BauI CACGAG 1 cut(s) 601
BbrPI CACGTG 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 913
BbvI GCAGC 4 cut(s) 65, 491, 753, 766
BccI CCATC 6 cut(s) 20, 26, 374, 451, 723, 742
BciT130I CCWGG 3 cut(s) 241, 853, 1072
BciVI GTATCC 1 cut(s) 299
BcoDI GTCTC 3 cut(s) 164, 447, 604
BfaI CTAG 1 cut(s) 672
BfuI GTATCC 1 cut(s) 299
BglI GCCNNNNNGGC 2 cut(s) 47, 154
BisI GCNGC 6 cut(s) 42, 51, 54, 480, 767, 780
BlpI GCTNAGC 1 cut(s) 245
BlsI GCNGC 6 cut(s) 43, 52, 55, 481, 768, 781
Bme1390I CCNGG 3 cut(s) 241, 853, 1072
BmiI GGNNCC 1 cut(s) 266
BmrFI CCNGG 3 cut(s) 241, 853, 1072
BmsI GCATC 2 cut(s) 556, 1057
BpmI CTGGAG 1 cut(s) 223
Bpu1102I GCTNAGC 1 cut(s) 245
BsaAI YACGTR 1 cut(s) 375
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 157
BsaJI CCNNGG 3 cut(s) 293, 426, 852
BsaWI WCCGGW 1 cut(s) 905
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 551
Bse118I RCCGGY 1 cut(s) 905
Bse3DI GCAATG 1 cut(s) 884
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 3 cut(s) 241, 853, 1072
BseDI CCNNGG 3 cut(s) 293, 426, 852
BseGI GGATG 7 cut(s) 18, 513, 523, 561, 734, 753, 830
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 299, 551
BseMI GCAATG 1 cut(s) 884
BseMII CTCAG 2 cut(s) 202, 259
BseRI GAGGAG 4 cut(s) 55, 226, 274, 707
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 4 cut(s) 65, 491, 753, 766
BseYI CCCAGC 1 cut(s) 94
Bsh1236I CGCG 2 cut(s) 24, 181
BshFI GGCC 2 cut(s) 50, 827
BshTI ACCGGT 1 cut(s) 905
BsiHKAI GWGCWC 1 cut(s) 913
BsiSI CCGG 1 cut(s) 906
BslFI GGGAC 1 cut(s) 766
BslI CCNNNNNNNGG 2 cut(s) 299, 551
BsmAI GTCTC 3 cut(s) 164, 447, 604
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 766
BsmI GAATGC 2 cut(s) 681, 782
BsnI GGCC 2 cut(s) 50, 827
Bsp1286I GDGCHC 1 cut(s) 913
Bsp1407I TGTACA 1 cut(s) 1042
Bsp143I GATC 3 cut(s) 418, 950, 1009
Bsp1720I GCTNAGC 1 cut(s) 245
BspANI GGCC 2 cut(s) 50, 827
BspCNI CTCAG 2 cut(s) 201, 258
BspFNI CGCG 2 cut(s) 24, 181
BspLI GGNNCC 1 cut(s) 266
BspPI GGATC 2 cut(s) 413, 958
BsrDI GCAATG 1 cut(s) 884
BsrFI RCCGGY 1 cut(s) 905
BsrGI TGTACA 1 cut(s) 1042
BssAI RCCGGY 1 cut(s) 905
BssECI CCNNGG 3 cut(s) 293, 426, 852
BssMI GATC 3 cut(s) 418, 950, 1009
BssNAI GTATAC 1 cut(s) 813
BssNI GRCGYC 1 cut(s) 157
BssSI CACGAG 1 cut(s) 601
BssT1I CCWWGG 1 cut(s) 293
Bst1107I GTATAC 1 cut(s) 813
Bst2BI CACGAG 1 cut(s) 601
Bst2UI CCWGG 3 cut(s) 241, 853, 1072
Bst4CI ACNGT 2 cut(s) 313, 866
Bst6I CTCTTC 3 cut(s) 134, 692, 1028
BstACI GRCGYC 1 cut(s) 157
BstAPI GCANNNNNTGC 1 cut(s) 999
BstAUI TGTACA 1 cut(s) 1042
BstBAI YACGTR 1 cut(s) 375
BstC8I GCNNGC 4 cut(s) 179, 185, 263, 764
BstDEI CTNAG 2 cut(s) 188, 245
BstF5I GGATG 7 cut(s) 18, 513, 523, 561, 734, 753, 830
BstFNI CGCG 2 cut(s) 24, 181
BstHHI GCGC 2 cut(s) 183, 646
BstKTI GATC 3 cut(s) 421, 953, 1012
BstMAI GTCTC 3 cut(s) 164, 447, 604
BstMBI GATC 3 cut(s) 418, 950, 1009
BstNI CCWGG 3 cut(s) 241, 853, 1072
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 3 cut(s) 239, 851, 1070
BstSLI GKGCMC 1 cut(s) 913
BstUI CGCG 2 cut(s) 24, 181
BstV1I GCAGC 4 cut(s) 65, 491, 753, 766
BstX2I RGATCY 1 cut(s) 950
BstYI RGATCY 1 cut(s) 950
BstZ17I GTATAC 1 cut(s) 813
BsuI GTATCC 1 cut(s) 299
BsuRI GGCC 2 cut(s) 50, 827
BtsCI GGATG 7 cut(s) 18, 513, 523, 561, 734, 753, 830
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 4 cut(s) 179, 185, 263, 764
CfoI GCGC 2 cut(s) 183, 646
Cfr10I RCCGGY 1 cut(s) 905
CseI GACGC 1 cut(s) 146
Csp6I GTAC 2 cut(s) 940, 1043
CspAI ACCGGT 1 cut(s) 905
CspCI CAANNNNNGTGG 2 cut(s) 155, 190
CviAII CATG 5 cut(s) 73, 256, 327, 398, 763
CviQI GTAC 2 cut(s) 940, 1043
DdeI CTNAG 2 cut(s) 188, 245
DpnI GATC 3 cut(s) 420, 952, 1011
DpnII GATC 3 cut(s) 418, 950, 1009
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 3 cut(s) 134, 692, 1028
EarI CTCTTC 3 cut(s) 134, 692, 1028
EciI GGCGGA 4 cut(s) 19, 96, 152, 411
Eco130I CCWWGG 1 cut(s) 293
Eco57I CTGAAG 2 cut(s) 115, 939
Eco72I CACGTG 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 1032
EcoRII CCWGG 3 cut(s) 239, 851, 1070
EcoT14I CCWWGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 293
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 5 cut(s) 76, 259, 330, 401, 766
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FaqI GGGAC 1 cut(s) 766
FatI CATG 5 cut(s) 72, 255, 326, 397, 762
FauI CCCGC 1 cut(s) 186
FblI GTMKAC 1 cut(s) 812
Fnu4HI GCNGC 6 cut(s) 42, 51, 54, 480, 767, 780
FokI GGATG 7 cut(s) 5, 520, 530, 568, 741, 760, 817
Fsp4HI GCNGC 6 cut(s) 42, 51, 54, 480, 767, 780
FspBI CTAG 1 cut(s) 672
GlaI GCGC 2 cut(s) 182, 645
GluI GCNGC 6 cut(s) 42, 51, 54, 480, 767, 780
GsaI CCCAGC 1 cut(s) 98
GsuI CTGGAG 1 cut(s) 223
HaeIII GGCC 2 cut(s) 50, 827
HapII CCGG 1 cut(s) 906
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 183, 646
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 5 cut(s) 76, 259, 330, 401, 766
Hin6I GCGC 2 cut(s) 181, 644
HinP1I GCGC 2 cut(s) 181, 644
HinfI GANTC 4 cut(s) 26, 84, 192, 988
HpaII CCGG 1 cut(s) 906
HphI GGTGA 2 cut(s) 841, 937
Hpy166II GTNNAC 4 cut(s) 6, 666, 813, 911
Hpy188III TCNNGA 4 cut(s) 449, 474, 839, 957
Hpy8I GTNNAC 4 cut(s) 6, 666, 813, 911
Hpy99I CGWCG 2 cut(s) 200, 227
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 2 cut(s) 313, 866
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 3 cut(s) 679, 782, 911
HpyF3I CTNAG 2 cut(s) 188, 245
HpySE526I ACGT 1 cut(s) 374
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 5 cut(s) 76, 259, 330, 401, 766
HspAI GCGC 2 cut(s) 181, 644
Kzo9I GATC 3 cut(s) 418, 950, 1009
LmnI GCTCC 1 cut(s) 270
Lsp1109I GCAGC 4 cut(s) 65, 491, 753, 766
LweI GCATC 2 cut(s) 556, 1057
MaeI CTAG 1 cut(s) 672
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 847
MalI GATC 3 cut(s) 420, 952, 1011
MboI GATC 3 cut(s) 418, 950, 1009
MboII GAAGA 7 cut(s) 121, 709, 881, 945, 946, 1015, 1041
MflI RGATCY 1 cut(s) 950
MhlI GDGCHC 1 cut(s) 913
MluCI AATT 3 cut(s) 712, 960, 1032
MlyI GAGTC 3 cut(s) 20, 186, 997
MmeI TCCRAC 1 cut(s) 93
Mph1103I ATGCAT 1 cut(s) 681
MseI TTAA 1 cut(s) 1056
MspI CCGG 1 cut(s) 906
MspR9I CCNGG 3 cut(s) 241, 853, 1072
Mva1269I GAATGC 2 cut(s) 681, 782
MvaI CCWGG 3 cut(s) 241, 853, 1072
MvnI CGCG 2 cut(s) 24, 181
NdeII GATC 3 cut(s) 418, 950, 1009
NlaIII CATG 5 cut(s) 76, 259, 330, 401, 766
NlaIV GGNNCC 1 cut(s) 266
NmuCI GTSAC 1 cut(s) 847
NsiI ATGCAT 1 cut(s) 681
NspI RCATGY 1 cut(s) 766
PaeI GCATGC 1 cut(s) 766
PctI GAATGC 2 cut(s) 681, 782
PfeI GAWTC 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 299
PinAI ACCGGT 1 cut(s) 905
PkrI GCNGC 6 cut(s) 43, 52, 55, 481, 768, 781
PleI GAGTC 3 cut(s) 20, 186, 996
PmaCI CACGTG 1 cut(s) 375
PmlI CACGTG 1 cut(s) 375
PpsI GAGTC 3 cut(s) 20, 186, 996
Ppu21I YACGTR 1 cut(s) 375
Psp6I CCWGG 3 cut(s) 239, 851, 1070
PspCI CACGTG 1 cut(s) 375
PspFI CCCAGC 1 cut(s) 94
PspGI CCWGG 3 cut(s) 239, 851, 1070
PspN4I GGNNCC 1 cut(s) 266
PsuI RGATCY 1 cut(s) 950
RsaI GTAC 2 cut(s) 941, 1044
RsaNI GTAC 2 cut(s) 940, 1043
SaqAI TTAA 1 cut(s) 1056
SatI GCNGC 6 cut(s) 42, 51, 54, 480, 767, 780
Sau3AI GATC 3 cut(s) 418, 950, 1009
SchI GAGTC 3 cut(s) 20, 186, 997
ScrFI CCNGG 3 cut(s) 241, 853, 1072
SduI GDGCHC 1 cut(s) 913
SfaNI GCATC 2 cut(s) 556, 1057
SgrAI CRCCGGYG 1 cut(s) 905
SphI GCATGC 1 cut(s) 766
Sse9I AATT 3 cut(s) 712, 960, 1032
SspMI CTAG 1 cut(s) 672
StyD4I CCNGG 3 cut(s) 239, 851, 1070
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 313, 866
TaiI ACGT 1 cut(s) 377
TaqI TCGA 1 cut(s) 1065
TasI AATT 3 cut(s) 712, 960, 1032
TatI WGTACW 1 cut(s) 1042
TauI GCSGC 2 cut(s) 44, 53
TfiI GAWTC 1 cut(s) 84
Tru1I TTAA 1 cut(s) 1056
Tru9I TTAA 1 cut(s) 1056
TscAI CASTG 1 cut(s) 316
TseFI GTSAC 1 cut(s) 847
TseI GCWGC 4 cut(s) 53, 479, 766, 779
Tsp45I GTSAC 1 cut(s) 847
TspDTI ATGAA 3 cut(s) 89, 386, 779
TspRI CASTG 1 cut(s) 316
Van91I CCANNNNNTGG 1 cut(s) 299
VneI GTGCAC 1 cut(s) 909
XapI RAATTY 1 cut(s) 1032
XceI RCATGY 1 cut(s) 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 43
XmiI GTMKAC 1 cut(s) 812
XspI CTAG 1 cut(s) 672
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.