RchiOBHm_Chr2g0109671

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0018725
Physical Location & Seq
Reverse (-)
21031864 .. 21038147
6284 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48348

Sequence Viewer

Length: 1917 bp
ATGCCTTGGGAGGTAAAACGCAGCGTTTTACTGTTACTGACGGTTTTGTTGTTTTGGTGCGAATTCAAGTCTGGGGAAGCGAAGGAGACTGACGTTTGGCGACTCTTCAGAGAAGCTCAAAAGAATATTCTGCATTTGAACCAGCATCGGCTTAAGGCCGTAGAGGATCTGAACAAAACAAACAGTGAGAAGCAGCTGCTGATTGATAAGATTGAGCAGTTGGAGCGGGAAAAGCAGGCTTCTGTTGGAAAGCCTCAAGATAGGCTCTCAAGTTGTTGGGAAGTACTCCTTCGGATAGACTCAATGGTCCTCACTAGAATGATTGCTGCCGGGGAGGCATCTGATTTGAGAAGGCTGGTCACGGATCATAAAATGAGTCTAGCTGAAGTTTTTGATGGCACCTTGCAGAAGACAGATGCCGAGCTTCTAGCTGAACTGAGACACTTCTTTGCAAGAAGCAGAAGGAATGGCTTTCACATTGTGCACATATGTACGGAAATGGCACCACTGGTCTCTGTTGGATCTTTGGCATCATATGTGACTGGCTTGTCTCGTGCACTACAGAGGAAGGGGCATTTGGTGGAGGTGATACTTCCAAAGTATTCAAGCCTGGACCTAGATGAAATTCAAGGGCTACAAGAGATTGAGGCAGAGTCCTTTTCATATTTCAATGGTCAACTTCATGGAAACAGAATTTGGACTGGTGTTGTCTATGGCATTGGAGTTACCTTAATTCAACCTCTAGAGTATTCAGAGTTTTTCAATCGTGAAAAGGTGTATGGCTACTCAGATGACTTTGAAAGGTTTACCTATTTCTCTCGTGCTTCATTAGATTATATTGTAAAGTGTGGAAAGCAACCTGATGTGATACATATCCACAACTGGGAGACTGCTATTATTGGGCCACTTTTCTGGGATATTTTTGCTAACCAGGGACTTGAAGGTACGAGAATCTTATTGACATGCCATGACCTGAATTCACAGTGCCTCGAGCATCCGGATAAACTAGCGTTATGCGGACTTGATCCTTCTTCACTTCACCGTCCTGATCGCTTGCAAGATAATACTAAGTCACATTTGGTTAATATTTTGAAGGGTGGAGTTGTTTACTCCAATAATGTTGTCATAATGTCCTCCGTACTATCAAAAGGCAGGGTTATACATACTCTGAGTCATGGGTTGGACCCTACCTTAAACATTCACAAGAACAAGTTGGTTGTTTCTCCTTGTGGGTTTGACAATTCCGCCTGGGATCCATCCAAGGACAGCTTTCTTCCCCGAAATTTCAATGTCGAAGATATGGAAGGGAAAGCAGTTTGCAAAGCTGCATTGCAGCAGCACCTTGGGTTATCGGAGCATGCTTCTACCATTCTTATTGGATGCATTGTGTCTGAAGATGTTGATCTGGAGAAGATGAGGGCAGTAATCATGAAAGCTTGCAAGAGCGATGTACAGTTCATCATCATGGGTACTAGCAAATTATCAAGTATACACAATCTTGGATCATTGCAGGAAACACTGAAGGATGAACAAGTGAAATTCGTAAATGAGTGTGATGCAGCAATAACACATTTGGTTTTTGCCGGATCTGATATTATCTTGTGCCAATCTTTTCATGACCCTGTTCTCCAAGTTCCATTAAAGGCTTTAAAGTACGGAGCTGCTCCAATTGCATTAAACTCCTCCCATGACAACTTCAGGAACTTTGCAGAACATGACTATGAGACTACTAATTTCTCACGTTTCATCAGCTCTACCTTTGGAAATATGTCCATAAGCGAAGTGCTGGATGAGATGAAAAACCACCCCTCGAAGTGGAAGAGAAAAATACTGGACGCCATGGAAATGGACTTCTCATGGGATGCTGAGTGCTGTGACGTCCACTGTTCTGCTTACACTGCTATAAAGAAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

638

Amino Acids

72.13

Weight (kDa)

6.06

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 159 - 383 9.3e-40 Starch synthase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66595
rosa_chinensis RchiOBHm_Chr2g0109671
rosa_multiflora Rmu_sc0000332.1_g000062 Rmu_sc0027285.1_g000001
rosa_rugosa Rorug02G0161800
rosa_samantha Rh2AG214100 Rh2BG224400 Rh2CG216400 Rh2DG219700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 305, 547
AatII GACGTC 1 cut(s) 1881
AccB1I GGYRCC 2 cut(s) 398, 502
AccBSI CCGCTC 1 cut(s) 226
AccI GTMKAC 1 cut(s) 1489
AccIII TCCGGA 1 cut(s) 997
AciI CCGC 3 cut(s) 226, 1017, 1245
AclWI GGATC 8 cut(s) 174, 372, 529, 1019, 1247, 1260, 1510, 1594
AcsI RAATTY 6 cut(s) 62, 624, 693, 976, 1282, 1538
AcuI CTGAAG 5 cut(s) 91, 405, 1413, 1541, 1681
AcyI GRCGYC 2 cut(s) 1836, 1878
AdeI CACNNNGTG 1 cut(s) 481
AfaI GTAC 7 cut(s) 285, 493, 946, 1140, 1452, 1471, 1655
AfiI CCNNNNNNNGG 2 cut(s) 883, 1815
AflII CTTAAG 1 cut(s) 152
AjnI CCWGG 3 cut(s) 609, 930, 1247
AjuI GAANNNNNNNTTGG 4 cut(s) 560, 592, 679, 711
Alw21I GWGCWC 2 cut(s) 486, 559
Alw26I GTCTC 6 cut(s) 80, 433, 517, 555, 881, 1718
Alw44I GTGCAC 2 cut(s) 482, 555
AlwI GGATC 8 cut(s) 174, 372, 529, 1019, 1247, 1260, 1510, 1594
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 989
Aor13HI TCCGGA 1 cut(s) 997
AoxI GGCC 2 cut(s) 156, 902
ApaLI GTGCAC 2 cut(s) 482, 555
ApeKI GCWGC 9 cut(s) 21, 193, 196, 326, 1325, 1333, 1336, 1559, 1661
ApoI RAATTY 6 cut(s) 62, 624, 693, 976, 1282, 1538
AspS9I GGNCC 4 cut(s) 307, 613, 902, 1183
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 2 cut(s) 598, 1031
AvaI CYCGRG 1 cut(s) 989
AvaII GGWCC 3 cut(s) 307, 613, 1183
BaeGI GKGCMC 2 cut(s) 486, 559
BamHI GGATCC 1 cut(s) 1252
BanI GGYRCC 2 cut(s) 398, 502
BauI CACGAG 2 cut(s) 552, 819
BbsI GAAGAC 1 cut(s) 416
Bbv12I GWGCWC 2 cut(s) 486, 559
BbvI GCAGC 9 cut(s) 33, 183, 205, 313, 1312, 1345, 1348, 1571, 1648
BccI CCATC 2 cut(s) 389, 1264
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 3 cut(s) 611, 932, 1249
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 6 cut(s) 80, 433, 517, 555, 881, 1718
BfaI CTAG 7 cut(s) 315, 380, 428, 617, 743, 1007, 1473
BfmI CTRYAG 1 cut(s) 560
BfrI CTTAAG 1 cut(s) 152
BglI GCCNNNNNGGC 1 cut(s) 335
BisI GCNGC 9 cut(s) 22, 194, 197, 327, 1326, 1334, 1337, 1560, 1662
BlsI GCNGC 9 cut(s) 23, 195, 198, 328, 1327, 1335, 1338, 1561, 1663
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 4 cut(s) 331, 611, 932, 1249
Bme18I GGWCC 3 cut(s) 307, 613, 1183
BmeT110I CYCGRG 1 cut(s) 989
BmgT120I GGNCC 4 cut(s) 307, 613, 902, 1183
BmiI GGNNCC 4 cut(s) 400, 504, 1185, 1254
BmrFI CCNGG 4 cut(s) 331, 611, 932, 1249
BmrI ACTGGG 1 cut(s) 892
BmsI GCATC 8 cut(s) 154, 347, 406, 539, 1003, 1370, 1546, 1852
BmuI ACTGGG 1 cut(s) 892
BpiI GAAGAC 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 1427
BpuEI CTTGAG 2 cut(s) 240, 253
BpuMI CCSGG 1 cut(s) 331
BsaBI GATNNNNATC 2 cut(s) 872, 1401
BsaHI GRCGYC 2 cut(s) 1836, 1878
BsaI GGTCTC 1 cut(s) 517
BsaJI CCNNGG 7 cut(s) 5, 330, 931, 1248, 1260, 1342, 1839
BsaWI WCCGGW 1 cut(s) 997
Bsc4I CCNNNNNNNGG 2 cut(s) 883, 1815
Bse1I ACTGG 5 cut(s) 513, 547, 706, 887, 1836
Bse3DI GCAATG 2 cut(s) 1328, 1505
Bse8I GATNNNNATC 2 cut(s) 872, 1401
BseAI TCCGGA 1 cut(s) 997
BseBI CCWGG 3 cut(s) 611, 932, 1249
BseDI CCNNGG 7 cut(s) 5, 330, 931, 1248, 1260, 1342, 1839
BseGI GGATG 6 cut(s) 994, 1256, 1385, 1531, 1795, 1867
BseJI GATNNNNATC 2 cut(s) 872, 1401
BseLI CCNNNNNNNGG 2 cut(s) 883, 1815
BseMI GCAATG 2 cut(s) 1328, 1505
BseMII CTCAG 4 cut(s) 428, 801, 1160, 1857
BseNI ACTGG 5 cut(s) 513, 547, 706, 887, 1836
BseRI GAGGAG 1 cut(s) 1672
BseSI GKGCMC 2 cut(s) 486, 559
BseXI GCAGC 9 cut(s) 33, 183, 205, 313, 1312, 1345, 1348, 1571, 1648
BshFI GGCC 2 cut(s) 158, 904
BshNI GGYRCC 2 cut(s) 398, 502
BsiHKAI GWGCWC 2 cut(s) 486, 559
BsiHKCI CYCGRG 1 cut(s) 989
BsiSI CCGG 3 cut(s) 330, 998, 1584
BslFI GGGAC 1 cut(s) 948
BslI CCNNNNNNNGG 2 cut(s) 883, 1815
BsmAI GTCTC 6 cut(s) 80, 433, 517, 555, 881, 1718
BsmFI GGGAC 1 cut(s) 948
BsnI GGCC 2 cut(s) 158, 904
Bso31I GGTCTC 1 cut(s) 517
BsoBI CYCGRG 1 cut(s) 989
Bsp1286I GDGCHC 2 cut(s) 486, 559
Bsp13I TCCGGA 1 cut(s) 997
Bsp1407I TGTACA 1 cut(s) 1450
Bsp143I GATC 9 cut(s) 166, 364, 521, 1024, 1048, 1252, 1402, 1502, 1586
Bsp19I CCATGG 1 cut(s) 1839
BspACI CCGC 3 cut(s) 226, 1017, 1245
BspANI GGCC 2 cut(s) 158, 904
BspCNI CTCAG 4 cut(s) 429, 800, 1161, 1858
BspEI TCCGGA 1 cut(s) 997
BspHI TCATGA 3 cut(s) 1428, 1615, 1913
BspLI GGNNCC 4 cut(s) 400, 504, 1185, 1254
BspPI GGATC 8 cut(s) 174, 372, 529, 1019, 1247, 1260, 1510, 1594
BspT107I GGYRCC 2 cut(s) 398, 502
BspTI CTTAAG 1 cut(s) 152
BspTNI GGTCTC 1 cut(s) 517
BsrBI CCGCTC 1 cut(s) 226
BsrDI GCAATG 2 cut(s) 1328, 1505
BsrGI TGTACA 1 cut(s) 1450
BsrI ACTGG 5 cut(s) 513, 547, 706, 887, 1836
BssECI CCNNGG 7 cut(s) 5, 330, 931, 1248, 1260, 1342, 1839
BssMI GATC 9 cut(s) 166, 364, 521, 1024, 1048, 1252, 1402, 1502, 1586
BssNAI GTATAC 1 cut(s) 1490
BssNI GRCGYC 2 cut(s) 1836, 1878
BssSI CACGAG 2 cut(s) 552, 819
BssT1I CCWWGG 4 cut(s) 5, 1260, 1342, 1839
Bst1107I GTATAC 1 cut(s) 1490
Bst2BI CACGAG 2 cut(s) 552, 819
Bst2UI CCWGG 3 cut(s) 611, 932, 1249
Bst4CI ACNGT 7 cut(s) 33, 43, 185, 984, 1043, 1455, 1886
Bst6I CTCTTC 2 cut(s) 110, 1814
BstACI GRCGYC 2 cut(s) 1836, 1878
BstAFI CTTAAG 1 cut(s) 152
BstAUI TGTACA 1 cut(s) 1450
BstC8I GCNNGC 4 cut(s) 237, 1055, 1359, 1438
BstDEI CTNAG 5 cut(s) 437, 787, 1068, 1169, 1866
BstDSI CCRYGG 1 cut(s) 1839
BstF5I GGATG 6 cut(s) 994, 1256, 1385, 1531, 1795, 1867
BstKTI GATC 9 cut(s) 169, 367, 524, 1027, 1051, 1255, 1405, 1505, 1589
BstMAI GTCTC 6 cut(s) 80, 433, 517, 555, 881, 1718
BstMBI GATC 9 cut(s) 166, 364, 521, 1024, 1048, 1252, 1402, 1502, 1586
BstMWI GCNNNNNNNGC 5 cut(s) 223, 232, 335, 1670, 1898
BstNI CCWGG 3 cut(s) 611, 932, 1249
BstNSI RCATGY 2 cut(s) 966, 1361
BstSCI CCNGG 4 cut(s) 329, 609, 930, 1247
BstSFI CTRYAG 1 cut(s) 560
BstSLI GKGCMC 2 cut(s) 486, 559
BstV1I GCAGC 9 cut(s) 33, 183, 205, 313, 1312, 1345, 1348, 1571, 1648
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 4 cut(s) 166, 521, 1252, 1586
BstXI CCANNNNNNTGG 2 cut(s) 912, 1846
BstYI RGATCY 4 cut(s) 166, 521, 1252, 1586
BstZ17I GTATAC 1 cut(s) 1490
BsuRI GGCC 2 cut(s) 158, 904
BtgI CCRYGG 1 cut(s) 1839
BtgZI GCGATG 1 cut(s) 1461
BtsCI GGATG 6 cut(s) 994, 1256, 1385, 1531, 1795, 1867
BtsI GCAGTG 1 cut(s) 1896
BtsIMutI CAGTG 6 cut(s) 190, 506, 989, 1517, 1882, 1896
Cac8I GCNNGC 4 cut(s) 237, 1055, 1359, 1438
CaiI CAGNNNCTG 1 cut(s) 199
CciI TCATGA 3 cut(s) 1428, 1615, 1913
Cfr13I GGNCC 4 cut(s) 307, 613, 902, 1183
CseI GACGC 1 cut(s) 1844
Csp6I GTAC 7 cut(s) 284, 492, 945, 1139, 1451, 1470, 1654
CviQI GTAC 7 cut(s) 284, 492, 945, 1139, 1451, 1470, 1654
DdeI CTNAG 5 cut(s) 437, 787, 1068, 1169, 1866
DpnI GATC 9 cut(s) 168, 366, 523, 1026, 1050, 1254, 1404, 1504, 1588
DpnII GATC 9 cut(s) 166, 364, 521, 1024, 1048, 1252, 1402, 1502, 1586
DraI TTTAAA 1 cut(s) 1650
DraIII CACNNNGTG 1 cut(s) 481
DrdI GACNNNNNNGTC 2 cut(s) 305, 547
DseDI GACNNNNNNGTC 2 cut(s) 305, 547
Eam1104I CTCTTC 2 cut(s) 110, 1814
EarI CTCTTC 2 cut(s) 110, 1814
EciI GGCGGA 1 cut(s) 1234
Eco130I CCWWGG 4 cut(s) 5, 1260, 1342, 1839
Eco31I GGTCTC 1 cut(s) 517
Eco47I GGWCC 3 cut(s) 307, 613, 1183
Eco57I CTGAAG 5 cut(s) 91, 405, 1413, 1541, 1681
Eco88I CYCGRG 1 cut(s) 989
EcoRI GAATTC 2 cut(s) 62, 976
EcoRII CCWGG 3 cut(s) 609, 930, 1247
EcoT14I CCWWGG 4 cut(s) 5, 1260, 1342, 1839
EcoT22I ATGCAT 1 cut(s) 1385
ErhI CCWWGG 4 cut(s) 5, 1260, 1342, 1839
FalI AAGNNNNNCTT 4 cut(s) 273, 305, 1253, 1285
FaqI GGGAC 1 cut(s) 948
FauI CCCGC 1 cut(s) 219
FauNDI CATATG 2 cut(s) 488, 535
FblI GTMKAC 1 cut(s) 1489
Fnu4HI GCNGC 9 cut(s) 22, 194, 197, 327, 1326, 1334, 1337, 1560, 1662
FokI GGATG 6 cut(s) 981, 1243, 1392, 1538, 1802, 1874
Fsp4HI GCNGC 9 cut(s) 22, 194, 197, 327, 1326, 1334, 1337, 1560, 1662
FspBI CTAG 7 cut(s) 315, 380, 428, 617, 743, 1007, 1473
GluI GCNGC 9 cut(s) 22, 194, 197, 327, 1326, 1334, 1337, 1560, 1662
GsuI CTGGAG 1 cut(s) 1427
HaeIII GGCC 2 cut(s) 158, 904
HapII CCGG 3 cut(s) 330, 998, 1584
HgaI GACGC 1 cut(s) 1844
Hin1I GRCGYC 2 cut(s) 1836, 1878
HincII GTYRAC 1 cut(s) 677
HindII GTYRAC 1 cut(s) 677
HindIII AAGCTT 1 cut(s) 1434
HinfI GANTC 6 cut(s) 102, 299, 376, 653, 951, 1171
HpaII CCGG 3 cut(s) 330, 998, 1584
HphI GGTGA 2 cut(s) 598, 1031
Hpy166II GTNNAC 7 cut(s) 484, 557, 677, 807, 1108, 1490, 1882
Hpy8I GTNNAC 7 cut(s) 484, 557, 677, 807, 1108, 1490, 1882
HpyCH4III ACNGT 7 cut(s) 33, 43, 185, 984, 1043, 1455, 1886
HpyCH4IV ACGT 3 cut(s) 93, 1741, 1878
HpyF10VI GCNNNNNNNGC 5 cut(s) 223, 232, 335, 1670, 1898
HpyF3I CTNAG 5 cut(s) 437, 787, 1068, 1169, 1866
HpySE526I ACGT 3 cut(s) 93, 1741, 1878
Hsp92I GRCGYC 2 cut(s) 1836, 1878
Kpn2I TCCGGA 1 cut(s) 997
Kzo9I GATC 9 cut(s) 166, 364, 521, 1024, 1048, 1252, 1402, 1502, 1586
LmnI GCTCC 4 cut(s) 223, 1354, 1658, 1669
Lsp1109I GCAGC 9 cut(s) 33, 183, 205, 313, 1312, 1345, 1348, 1571, 1648
LweI GCATC 8 cut(s) 154, 347, 406, 539, 1003, 1370, 1546, 1852
MaeI CTAG 7 cut(s) 315, 380, 428, 617, 743, 1007, 1473
MaeII ACGT 3 cut(s) 93, 1741, 1878
MaeIII GTNAC 6 cut(s) 33, 358, 538, 724, 1071, 1874
MalI GATC 9 cut(s) 168, 366, 523, 1026, 1050, 1254, 1404, 1504, 1588
MbiI CCGCTC 1 cut(s) 226
MboI GATC 9 cut(s) 166, 364, 521, 1024, 1048, 1252, 1402, 1502, 1586
MboII GAAGA 8 cut(s) 97, 421, 1023, 1265, 1307, 1406, 1423, 1831
MfeI CAATTG 1 cut(s) 1668
MflI RGATCY 4 cut(s) 166, 521, 1252, 1586
MhlI GDGCHC 2 cut(s) 486, 559
MlyI GAGTC 5 cut(s) 96, 293, 385, 662, 1180
MmeI TCCRAC 4 cut(s) 201, 226, 499, 1161
Mph1103I ATGCAT 1 cut(s) 1385
MroI TCCGGA 1 cut(s) 997
MseI TTAA 7 cut(s) 153, 731, 1083, 1193, 1640, 1649, 1676
MslI CAYNNNNRTG 4 cut(s) 317, 1463, 1719, 1844
MspA1I CMGCKG 1 cut(s) 196
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 3 cut(s) 330, 998, 1584
MspR9I CCNGG 4 cut(s) 331, 611, 932, 1249
MunI CAATTG 1 cut(s) 1668
MvaI CCWGG 3 cut(s) 611, 932, 1249
MwoI GCNNNNNNNGC 5 cut(s) 223, 232, 335, 1670, 1898
NciI CCSGG 1 cut(s) 331
NcoI CCATGG 1 cut(s) 1839
NdeI CATATG 2 cut(s) 488, 535
NdeII GATC 9 cut(s) 166, 364, 521, 1024, 1048, 1252, 1402, 1502, 1586
NlaIV GGNNCC 4 cut(s) 400, 504, 1185, 1254
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 4 cut(s) 358, 538, 1071, 1874
NsiI ATGCAT 1 cut(s) 1385
NspI RCATGY 2 cut(s) 966, 1361
PaeI GCATGC 1 cut(s) 1361
PaeR7I CTCGAG 1 cut(s) 989
PagI TCATGA 3 cut(s) 1428, 1615, 1913
PfeI GAWTC 1 cut(s) 951
PkrI GCNGC 9 cut(s) 23, 195, 198, 328, 1327, 1335, 1338, 1561, 1663
PleI GAGTC 5 cut(s) 96, 293, 384, 661, 1179
PpsI GAGTC 5 cut(s) 96, 293, 384, 661, 1179
Psp6I CCWGG 3 cut(s) 609, 930, 1247
PspGI CCWGG 3 cut(s) 609, 930, 1247
PspN4I GGNNCC 4 cut(s) 400, 504, 1185, 1254
PspPI GGNCC 4 cut(s) 307, 613, 902, 1183
PspXI VCTCGAGB 1 cut(s) 989
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 4 cut(s) 166, 521, 1252, 1586
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 7 cut(s) 285, 493, 946, 1140, 1452, 1471, 1655
RsaNI GTAC 7 cut(s) 284, 492, 945, 1139, 1451, 1470, 1654
RseI CAYNNNNRTG 4 cut(s) 317, 1463, 1719, 1844
SaqAI TTAA 7 cut(s) 153, 731, 1083, 1193, 1640, 1649, 1676
SatI GCNGC 9 cut(s) 22, 194, 197, 327, 1326, 1334, 1337, 1560, 1662
Sau3AI GATC 9 cut(s) 166, 364, 521, 1024, 1048, 1252, 1402, 1502, 1586
Sau96I GGNCC 4 cut(s) 307, 613, 902, 1183
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 5 cut(s) 96, 293, 385, 662, 1180
ScrFI CCNGG 4 cut(s) 331, 611, 932, 1249
SduI GDGCHC 2 cut(s) 486, 559
SfaNI GCATC 8 cut(s) 154, 347, 406, 539, 1003, 1370, 1546, 1852
SfcI CTRYAG 1 cut(s) 560
Sfr274I CTCGAG 1 cut(s) 989
SinI GGWCC 3 cut(s) 307, 613, 1183
SlaI CTCGAG 1 cut(s) 989
SmiMI CAYNNNNRTG 4 cut(s) 317, 1463, 1719, 1844
SmlI CTYRAG 4 cut(s) 152, 255, 268, 989
SmoI CTYRAG 4 cut(s) 152, 255, 268, 989
SphI GCATGC 1 cut(s) 1361
SsiI CCGC 3 cut(s) 226, 1017, 1245
SspI AATATT 2 cut(s) 127, 1087
SspMI CTAG 7 cut(s) 315, 380, 428, 617, 743, 1007, 1473
StyD4I CCNGG 4 cut(s) 329, 609, 930, 1247
StyI CCWWGG 4 cut(s) 5, 1260, 1342, 1839
TaaI ACNGT 7 cut(s) 33, 43, 185, 984, 1043, 1455, 1886
TaiI ACGT 3 cut(s) 96, 1744, 1881
TaqI TCGA 3 cut(s) 990, 1293, 1811
TatI WGTACW 2 cut(s) 283, 1450
TfiI GAWTC 1 cut(s) 951
Tru1I TTAA 7 cut(s) 153, 731, 1083, 1193, 1640, 1649, 1676
Tru9I TTAA 7 cut(s) 153, 731, 1083, 1193, 1640, 1649, 1676
TscAI CASTG 6 cut(s) 190, 513, 989, 1524, 1889, 1903
TseFI GTSAC 4 cut(s) 358, 538, 1071, 1874
TseI GCWGC 9 cut(s) 21, 193, 196, 326, 1325, 1333, 1336, 1559, 1661
Tsp45I GTSAC 4 cut(s) 358, 538, 1071, 1874
TspGWI ACGGA 4 cut(s) 377, 509, 1126, 1671
TspRI CASTG 6 cut(s) 190, 513, 989, 1524, 1889, 1903
Vha464I CTTAAG 1 cut(s) 152
VneI GTGCAC 2 cut(s) 482, 555
VpaK11BI GGWCC 3 cut(s) 307, 613, 1183
XapI RAATTY 6 cut(s) 62, 624, 693, 976, 1282, 1538
XbaI TCTAGA 1 cut(s) 742
XceI RCATGY 2 cut(s) 966, 1361
XhoI CTCGAG 1 cut(s) 989
XmiI GTMKAC 1 cut(s) 1489
XspI CTAG 7 cut(s) 315, 380, 428, 617, 743, 1007, 1473
ZraI GACGTC 1 cut(s) 1879
ZrmI AGTACT 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 1385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.