Rmu_sc0027285.1_g000001

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027285.1
OG0018725
Physical Location & Seq
Reverse (-)
1 .. 2350
2350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027285.1_g000001.1.cds

Sequence Viewer

Length: 911 bp
atgaaggcggcgttgatcagcaccgccattccttggcagcctccgccgcaccataagcctctcaaacccctttgttgctccaaggttaacggagcctcctcctcgccgtcgccgtcgccgtcatcctcctcacaaatcaatttcaggttgcaaggcagcgaaaatgctgagctccctcagcctcggattcaggagactgacgtttggcgactcttcagagaagctcagaagaatattctgcatttgaaccagcatcggcttaaggccgtagaggatctgaacaaaacaaacagtgagaagcagctgctgattgataagattgagcagttggagcgggaaaagcaggcttctgttggaaagcctcaagataggctctcaagttgttgggaagtactccttcggatagactcaatggtcctcactagaatgattgctgccggggaggcatctgatttgagaaggctggtcatggatcataaagttagtctagctgaagtttttgatggcaccttgcagaagacagatgctgagcttctagctgaactgagacacttctttgcaagaagccgaaggaatggctttcacattgtgcacatatgtacggaaatggcaccactggtctctgttggatctttggcatcatatgtgactggcttgtctcgtgcactacagaggaaggggcatttggtggaggtgatacttccaaagtattcaagcctggacctagatgaaattcaagggctacaagagattgaggcagagtccttttcatatttcaatggtcaacttcatggaaacagaatttggactggtgttgtctatggcattggagttaccttaattcaacctctagagtattcagagtttttcaatcgtgaaaaggtgtatggctactcagatgactttgaaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

303

Amino Acids

34.23

Weight (kDa)

6.57

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66595
rosa_chinensis RchiOBHm_Chr2g0109671
rosa_multiflora Rmu_sc0000332.1_g000062 Rmu_sc0027285.1_g000001
rosa_rugosa Rorug02G0161800
rosa_samantha Rh2AG214100 Rh2BG224400 Rh2CG216400 Rh2DG219700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 413, 655
AccB1I GGYRCC 2 cut(s) 506, 610
AccB7I CCANNNNNTGG 1 cut(s) 33
AccBSI CCGCTC 1 cut(s) 334
AciI CCGC 5 cut(s) 8, 24, 44, 47, 334
AclWI GGATC 3 cut(s) 282, 480, 637
AcsI RAATTY 2 cut(s) 732, 801
AcuI CTGAAG 2 cut(s) 199, 513
AdeI CACNNNGTG 1 cut(s) 589
AfaI GTAC 2 cut(s) 393, 601
AfiI CCNNNNNNNGG 1 cut(s) 33
AflII CTTAAG 1 cut(s) 260
AgsI TTSAA 7 cut(s) 247, 714, 737, 778, 845, 871, 908
AjnI CCWGG 1 cut(s) 717
AjuI GAANNNNNNNTTGG 4 cut(s) 668, 700, 787, 819
AluBI AGCT 6 cut(s) 172, 224, 304, 491, 532, 539
AluI AGCT 6 cut(s) 172, 224, 304, 491, 532, 539
Alw21I GWGCWC 3 cut(s) 174, 594, 667
Alw26I GTCTC 4 cut(s) 188, 541, 625, 663
Alw44I GTGCAC 2 cut(s) 590, 663
AlwI GGATC 3 cut(s) 282, 480, 637
AlwNI CAGNNNCTG 2 cut(s) 307, 527
AoxI GGCC 1 cut(s) 264
ApaLI GTGCAC 2 cut(s) 590, 663
ApeKI GCWGC 5 cut(s) 37, 156, 301, 304, 434
ApoI RAATTY 2 cut(s) 732, 801
AspS9I GGNCC 2 cut(s) 415, 721
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 1 cut(s) 706
AvaII GGWCC 2 cut(s) 415, 721
BaeGI GKGCMC 2 cut(s) 594, 667
BanI GGYRCC 2 cut(s) 506, 610
BanII GRGCYC 1 cut(s) 174
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 3 cut(s) 174, 594, 667
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 5 cut(s) 49, 168, 291, 313, 421
BccI CCATC 1 cut(s) 497
BceAI ACGGC 4 cut(s) 91, 97, 103, 251
BciT130I CCWGG 1 cut(s) 719
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 1 cut(s) 439
BcoDI GTCTC 4 cut(s) 188, 541, 625, 663
BfaI CTAG 5 cut(s) 423, 488, 536, 725, 851
BfmI CTRYAG 1 cut(s) 668
BfrI CTTAAG 1 cut(s) 260
BglI GCCNNNNNGGC 1 cut(s) 443
BisI GCNGC 7 cut(s) 9, 38, 47, 157, 302, 305, 435
BlpI GCTNAGC 2 cut(s) 168, 528
BlsI GCNGC 7 cut(s) 10, 39, 48, 158, 303, 306, 436
BmcAI AGTACT 1 cut(s) 393
Bme1390I CCNGG 2 cut(s) 439, 719
Bme18I GGWCC 2 cut(s) 415, 721
BmgT120I GGNCC 2 cut(s) 415, 721
BmiI GGNNCC 3 cut(s) 94, 508, 612
BmrFI CCNGG 2 cut(s) 439, 719
BmsI GCATC 4 cut(s) 262, 455, 514, 647
BpiI GAAGAC 1 cut(s) 524
Bpu10I CCTNAGC 1 cut(s) 177
Bpu1102I GCTNAGC 2 cut(s) 168, 528
BpuEI CTTGAG 2 cut(s) 348, 361
BpuMI CCSGG 1 cut(s) 439
BsaI GGTCTC 1 cut(s) 625
BsaJI CCNNGG 4 cut(s) 32, 81, 182, 438
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse1I ACTGG 3 cut(s) 621, 655, 814
BseBI CCWGG 1 cut(s) 719
BseDI CCNNGG 4 cut(s) 32, 81, 182, 438
BseGI GGATG 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMII CTCAG 6 cut(s) 159, 191, 239, 519, 536, 909
BseNI ACTGG 3 cut(s) 621, 655, 814
BseRI GAGGAG 3 cut(s) 88, 91, 118
BseSI GKGCMC 2 cut(s) 594, 667
BseXI GCAGC 5 cut(s) 49, 168, 291, 313, 421
BshFI GGCC 1 cut(s) 266
BshNI GGYRCC 2 cut(s) 506, 610
BsiHKAI GWGCWC 3 cut(s) 174, 594, 667
BsiSI CCGG 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 4 cut(s) 188, 541, 625, 663
BsnI GGCC 1 cut(s) 266
Bso31I GGTCTC 1 cut(s) 625
Bsp1286I GDGCHC 3 cut(s) 174, 594, 667
Bsp143I GATC 4 cut(s) 15, 274, 472, 629
Bsp1720I GCTNAGC 2 cut(s) 168, 528
BspACI CCGC 5 cut(s) 8, 24, 44, 47, 334
BspANI GGCC 1 cut(s) 266
BspCNI CTCAG 6 cut(s) 160, 190, 238, 520, 537, 908
BspLI GGNNCC 3 cut(s) 94, 508, 612
BspPI GGATC 3 cut(s) 282, 480, 637
BspT107I GGYRCC 2 cut(s) 506, 610
BspTI CTTAAG 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 625
BsrBI CCGCTC 1 cut(s) 334
BsrI ACTGG 3 cut(s) 621, 655, 814
BssECI CCNNGG 4 cut(s) 32, 81, 182, 438
BssMI GATC 4 cut(s) 15, 274, 472, 629
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 2 cut(s) 32, 81
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 1 cut(s) 719
Bst4CI ACNGT 1 cut(s) 293
Bst6I CTCTTC 1 cut(s) 218
BstAFI CTTAAG 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 6 cut(s) 168, 177, 225, 528, 545, 895
BstF5I GGATG 1 cut(s) 122
BstKTI GATC 4 cut(s) 18, 277, 475, 632
BstMAI GTCTC 4 cut(s) 188, 541, 625, 663
BstMBI GATC 4 cut(s) 15, 274, 472, 629
BstMWI GCNNNNNNNGC 7 cut(s) 43, 46, 55, 178, 331, 340, 443
BstNI CCWGG 1 cut(s) 719
BstSCI CCNGG 2 cut(s) 437, 717
BstSFI CTRYAG 1 cut(s) 668
BstSLI GKGCMC 2 cut(s) 594, 667
BstV1I GCAGC 5 cut(s) 49, 168, 291, 313, 421
BstV2I GAAGAC 1 cut(s) 524
BstX2I RGATCY 2 cut(s) 274, 629
BstYI RGATCY 2 cut(s) 274, 629
BsuRI GGCC 1 cut(s) 266
BtsCI GGATG 1 cut(s) 122
BtsIMutI CAGTG 2 cut(s) 298, 614
Cac8I GCNNGC 1 cut(s) 345
CaiI CAGNNNCTG 2 cut(s) 307, 527
Cfr13I GGNCC 2 cut(s) 415, 721
Csp6I GTAC 2 cut(s) 392, 600
CviAII CATG 2 cut(s) 469, 791
CviQI GTAC 2 cut(s) 392, 600
DdeI CTNAG 6 cut(s) 168, 177, 225, 528, 545, 895
DpnI GATC 4 cut(s) 17, 276, 474, 631
DpnII GATC 4 cut(s) 15, 274, 472, 629
DraIII CACNNNGTG 1 cut(s) 589
DrdI GACNNNNNNGTC 2 cut(s) 413, 655
DseDI GACNNNNNNGTC 2 cut(s) 413, 655
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 32, 81
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 625
Eco47I GGWCC 2 cut(s) 415, 721
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 199, 513
EcoICRI GAGCTC 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 717
EcoT14I CCWWGG 2 cut(s) 32, 81
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 32, 81
FaeI CATG 2 cut(s) 472, 794
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 2 cut(s) 468, 790
FauI CCCGC 1 cut(s) 327
FauNDI CATATG 2 cut(s) 596, 643
FbaI TGATCA 1 cut(s) 15
Fnu4HI GCNGC 7 cut(s) 9, 38, 47, 157, 302, 305, 435
FokI GGATG 1 cut(s) 109
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 7 cut(s) 9, 38, 47, 157, 302, 305, 435
FspBI CTAG 5 cut(s) 423, 488, 536, 725, 851
GluI GCNGC 7 cut(s) 9, 38, 47, 157, 302, 305, 435
HaeIII GGCC 1 cut(s) 266
HapII CCGG 1 cut(s) 438
Hin1II CATG 2 cut(s) 472, 794
HincII GTYRAC 2 cut(s) 88, 785
HindII GTYRAC 2 cut(s) 88, 785
HinfI GANTC 4 cut(s) 187, 210, 407, 761
HpaI GTTAAC 1 cut(s) 88
HpaII CCGG 1 cut(s) 438
HphI GGTGA 1 cut(s) 706
Hpy166II GTNNAC 4 cut(s) 88, 592, 665, 785
Hpy188I TCNGA 8 cut(s) 186, 218, 228, 279, 402, 451, 862, 898
Hpy188III TCNNGA 4 cut(s) 191, 365, 851, 875
Hpy8I GTNNAC 4 cut(s) 88, 592, 665, 785
Hpy99I CGWCG 2 cut(s) 112, 118
HpyAV CCTTC 4 cut(s) 407, 453, 564, 670
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 6 cut(s) 151, 241, 514, 560, 592, 665
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 46, 55, 178, 331, 340, 443
HpyF3I CTNAG 6 cut(s) 168, 177, 225, 528, 545, 895
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 2 cut(s) 472, 794
Ksp22I TGATCA 1 cut(s) 15
KspAI GTTAAC 1 cut(s) 88
Kzo9I GATC 4 cut(s) 15, 274, 472, 629
LmnI GCTCC 4 cut(s) 83, 92, 177, 331
Lsp1109I GCAGC 5 cut(s) 49, 168, 291, 313, 421
LweI GCATC 4 cut(s) 262, 455, 514, 647
MaeI CTAG 5 cut(s) 423, 488, 536, 725, 851
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 646, 832
MalI GATC 4 cut(s) 17, 276, 474, 631
MbiI CCGCTC 1 cut(s) 334
MboI GATC 4 cut(s) 15, 274, 472, 629
MboII GAAGA 3 cut(s) 205, 241, 529
MflI RGATCY 2 cut(s) 274, 629
MhlI GDGCHC 3 cut(s) 174, 594, 667
MluCI AATT 4 cut(s) 139, 732, 801, 840
MlyI GAGTC 3 cut(s) 204, 401, 770
MmeI TCCRAC 3 cut(s) 309, 334, 607
MseI TTAA 3 cut(s) 87, 261, 839
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 304
MspCI CTTAAG 1 cut(s) 260
MspI CCGG 1 cut(s) 438
MspR9I CCNGG 2 cut(s) 439, 719
MvaI CCWGG 1 cut(s) 719
MwoI GCNNNNNNNGC 7 cut(s) 43, 46, 55, 178, 331, 340, 443
NciI CCSGG 1 cut(s) 439
NdeI CATATG 2 cut(s) 596, 643
NdeII GATC 4 cut(s) 15, 274, 472, 629
NlaIII CATG 2 cut(s) 472, 794
NlaIV GGNNCC 3 cut(s) 94, 508, 612
NmuCI GTSAC 1 cut(s) 646
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 116
PfeI GAWTC 1 cut(s) 187
PflMI CCANNNNNTGG 1 cut(s) 33
PkrI GCNGC 7 cut(s) 10, 39, 48, 158, 303, 306, 436
PleI GAGTC 3 cut(s) 204, 401, 769
PpsI GAGTC 3 cut(s) 204, 401, 769
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 717
PspGI CCWGG 1 cut(s) 717
PspN4I GGNNCC 3 cut(s) 94, 508, 612
PspPI GGNCC 2 cut(s) 415, 721
PstNI CAGNNNCTG 2 cut(s) 307, 527
PsuI RGATCY 2 cut(s) 274, 629
PvuII CAGCTG 1 cut(s) 304
RsaI GTAC 2 cut(s) 393, 601
RsaNI GTAC 2 cut(s) 392, 600
RseI CAYNNNNRTG 1 cut(s) 425
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 3 cut(s) 87, 261, 839
SatI GCNGC 7 cut(s) 9, 38, 47, 157, 302, 305, 435
Sau3AI GATC 4 cut(s) 15, 274, 472, 629
Sau96I GGNCC 2 cut(s) 415, 721
ScaI AGTACT 1 cut(s) 393
SchI GAGTC 3 cut(s) 204, 401, 770
ScrFI CCNGG 2 cut(s) 439, 719
SduI GDGCHC 3 cut(s) 174, 594, 667
SfaNI GCATC 4 cut(s) 262, 455, 514, 647
SfcI CTRYAG 1 cut(s) 668
SinI GGWCC 2 cut(s) 415, 721
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 3 cut(s) 260, 363, 376
SmoI CTYRAG 3 cut(s) 260, 363, 376
Sse9I AATT 4 cut(s) 139, 732, 801, 840
SsiI CCGC 5 cut(s) 8, 24, 44, 47, 334
SspI AATATT 1 cut(s) 235
SspMI CTAG 5 cut(s) 423, 488, 536, 725, 851
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 2 cut(s) 437, 717
StyI CCWWGG 2 cut(s) 32, 81
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 1 cut(s) 204
TasI AATT 4 cut(s) 139, 732, 801, 840
TatI WGTACW 1 cut(s) 391
TauI GCSGC 2 cut(s) 11, 49
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 3 cut(s) 87, 261, 839
Tru9I TTAA 3 cut(s) 87, 261, 839
TscAI CASTG 2 cut(s) 298, 621
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 5 cut(s) 37, 156, 301, 304, 434
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 4 cut(s) 17, 744, 759, 779
TspGWI ACGGA 2 cut(s) 105, 617
TspRI CASTG 2 cut(s) 298, 621
Van91I CCANNNNNTGG 1 cut(s) 33
Vha464I CTTAAG 1 cut(s) 260
VneI GTGCAC 2 cut(s) 590, 663
VpaK11BI GGWCC 2 cut(s) 415, 721
XapI RAATTY 2 cut(s) 732, 801
XbaI TCTAGA 1 cut(s) 850
XspI CTAG 5 cut(s) 423, 488, 536, 725, 851
ZrmI AGTACT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.