Rh2DG219700

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018725
Physical Location & Seq
Reverse (-)
21111517 .. 21120472
8956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG219700.1

Sequence Viewer

Length: 2034 bp
ATGAAGGCGGCGTTGATCAGCACCGCCATTCCTTGGCAGCCTCCGCCGCACCATAAGCCTCTCAAACCCCTTTGTTGCTCCAAGGTTAACGGAGCCTCCTCCTCGCCGTCGCCGTCGCCGTCATCCTCCTCACAAATCAAGTTGCAAGGCAGCGAAAATGCTGAGCTCCCTCAGCCTCGGATTCAGTCTGGGGAAGCGAAGGAGACTGACGTTTGGCGACTCTTCAGAGAAGCTCAGAAGAATATTCTGCATTTGAACCAGCATCGGCTTAAGGCCGTAGAGGATCTGAACAAAACAAACAGTGAGAAGCAGCTGCTGATTGATAAGATTGAGCAGTTGGAGCGGGAAAAGCAGGCTTCTGTTGGAAAGCCTCAAGATAGGCTCTCAAGTTGTTGGGAAGTACTCCTTCGGATAGACTCAATGGTCCTCACTAGAATGATTGCTGCCGGGGAGGCATCTGATTTGAGAAGGCTGGTCATGGATCATAAAGTTAGTCTAGCTGAAGTTTTTGATGGCACCTTGCAGAAGACAGATGCCGAGCTTCTAGCTGAACTGAGACACTTATTTGCAAGAAGCAGAAGGAATGGCTTTCACATTGTGCACATATGTACGGAAATGGCACCACTGGTCTCTGTTGGATCTTTGGCATCATATGTGACTGGCTTGTCTCGTGCACTACAGAGGAAGGGGCATTTGGTGGAGGTGATACTTCCAAAGTATTCAAGCCTGGACCTTGATGAAATTCAAGGGCTACAAGAGATTGAGGCACAGTCCTTTTCATATTTCAATGGTCAACTTCATGGAAACAGAATTTGGACTGGTGTTGTCTATGGCATTGGAGTTACCTTAATTCAACCTCTAGAGTATTCAGAGTTTTTCAATCGTGAAAAGGTGTATGGCTACTCAGATGACTTTGAAAGGTTTACCTATTTCTCTCGTGCTTCATTAGATTATATTGTAAAGTGTGGAAAGCAACCTGATGTGATACATATCCACAACTGGGAGACTGCTATTATTGGGCCACTTTTCTGGGATATTTTTGCTAACCAGGGACTTGAAGGTACGAGAATCTTATTGACATGCCATGACCTGAATTCACAGTGCCTCGAGCATCCGGATAAACTAGCGTTATGCGGACTTGATCCTTCTTCACTTCACCGTCCTGATCGCTTGCAAGATAATACTAAGTCACATTTGGTTAATATTTTGAAGGGTGGAGTTGTTTACTCCAATAATGTTGTCATAATGTCTTCCATACTATCAAAAGGCAGGGTTATACATGCTCTGAGTCATGGGTTGGACCCTACCTTAAACATTCACAAGAACAAGTTGGTTGTTTCTCCTTGTGGATTTGACAATTTCACCTGGGATCCATCCAAGGACAGTTTTCTTCCCCGAAATTTCAATGTCGAAGATATGGAAGGGAAAGCAGTTTGCAAAGCTGCATTGCAGCAGCACCTTGGGTTATCGGAGCATGCTTCTACCATTCTTATTGGATGCATTGTGTCTGAAGATGTTGATCTGGAGAACATGAGGGCAGTAATCATGAAAGCTTGCAAGAGCGATGTACAGTTCATCATCATGGGTACTAGCAAATTATCAAGTATACACAAGCTTGGATCATTGCAGGAACCACTGAAGGATGAACAAGTGAAATTCGTAAATGAGTGTGATGCAGCAATAACACATTTGGTTTTTGCCGGATCTGATATTATCTTGTGCCAATCTTTTCATGACCCTGTTCTCCAAGTTCCATTAAAGGCTTTAAAGTACGGAGCTGCTCCAATTGCATTAAACTCCTCCCATGACAACTTCAGGAACTTTGCAGAACATGACTATGAGACTACTAATTTCTCACGTTTCATCAGCTCTACCTTTGGAAATATGTCCATAAGCGAAGTGCTGGATGAGATGAAAAACCACCCCTCGAAGTGGAAGAGAAAAATACTGGACGCCATGGAAATGGACTTCTCATGGGATGCTGAGTGCTGTGACGTCCACTGTTCTGCTTACACTGCTATAAAGAAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

677

Amino Acids

75.85

Weight (kDa)

6.34

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 198 - 422 7.9e-40 Starch synthase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66595
rosa_chinensis RchiOBHm_Chr2g0109671
rosa_multiflora Rmu_sc0000332.1_g000062 Rmu_sc0027285.1_g000001
rosa_rugosa Rorug02G0161800
rosa_samantha Rh2AG214100 Rh2BG224400 Rh2CG216400 Rh2DG219700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 422, 664
AatII GACGTC 1 cut(s) 1998
AccB1I GGYRCC 2 cut(s) 515, 619
AccB7I CCANNNNNTGG 1 cut(s) 33
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 1606
AccIII TCCGGA 1 cut(s) 1114
AciI CCGC 6 cut(s) 8, 24, 44, 47, 343, 1134
AclWI GGATC 8 cut(s) 291, 489, 646, 1136, 1364, 1377, 1627, 1711
AcsI RAATTY 5 cut(s) 741, 810, 1093, 1399, 1655
AcuI CTGAAG 5 cut(s) 208, 522, 1530, 1658, 1798
AcyI GRCGYC 2 cut(s) 1953, 1995
AdeI CACNNNGTG 1 cut(s) 598
AfaI GTAC 6 cut(s) 402, 610, 1063, 1569, 1588, 1772
AfiI CCNNNNNNNGG 3 cut(s) 33, 1000, 1932
AflII CTTAAG 1 cut(s) 269
AjnI CCWGG 3 cut(s) 726, 1047, 1364
AjuI GAANNNNNNNTTGG 4 cut(s) 677, 709, 796, 828
Alw21I GWGCWC 3 cut(s) 168, 603, 676
Alw26I GTCTC 6 cut(s) 197, 550, 634, 672, 998, 1835
Alw44I GTGCAC 2 cut(s) 599, 672
AlwI GGATC 8 cut(s) 291, 489, 646, 1136, 1364, 1377, 1627, 1711
AlwNI CAGNNNCTG 1 cut(s) 316
Ama87I CYCGRG 1 cut(s) 1106
Aor13HI TCCGGA 1 cut(s) 1114
AoxI GGCC 2 cut(s) 273, 1019
ApaLI GTGCAC 2 cut(s) 599, 672
ApoI RAATTY 5 cut(s) 741, 810, 1093, 1399, 1655
AspS9I GGNCC 4 cut(s) 424, 730, 1019, 1300
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 3 cut(s) 715, 1148, 1354
AvaI CYCGRG 1 cut(s) 1106
AvaII GGWCC 3 cut(s) 424, 730, 1300
BaeGI GKGCMC 2 cut(s) 603, 676
BamHI GGATCC 1 cut(s) 1369
BanI GGYRCC 2 cut(s) 515, 619
BanII GRGCYC 1 cut(s) 168
BauI CACGAG 2 cut(s) 669, 936
BbsI GAAGAC 2 cut(s) 533, 1242
Bbv12I GWGCWC 3 cut(s) 168, 603, 676
BbvCI CCTCAGC 1 cut(s) 171
BccI CCATC 2 cut(s) 506, 1381
BceAI ACGGC 4 cut(s) 91, 97, 103, 260
BciT130I CCWGG 3 cut(s) 728, 1049, 1366
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 6 cut(s) 197, 550, 634, 672, 998, 1835
BfaI CTAG 6 cut(s) 432, 497, 545, 860, 1124, 1590
BfmI CTRYAG 1 cut(s) 677
BfrI CTTAAG 1 cut(s) 269
BglI GCCNNNNNGGC 1 cut(s) 452
BlpI GCTNAGC 1 cut(s) 162
BmcAI AGTACT 1 cut(s) 402
Bme1390I CCNGG 4 cut(s) 448, 728, 1049, 1366
Bme18I GGWCC 3 cut(s) 424, 730, 1300
BmeT110I CYCGRG 1 cut(s) 1106
BmgT120I GGNCC 4 cut(s) 424, 730, 1019, 1300
BmiI GGNNCC 6 cut(s) 94, 517, 621, 1302, 1371, 1632
BmrFI CCNGG 4 cut(s) 448, 728, 1049, 1366
BmrI ACTGGG 1 cut(s) 1009
BmsI GCATC 8 cut(s) 271, 464, 523, 656, 1120, 1487, 1663, 1969
BmuI ACTGGG 1 cut(s) 1009
BpiI GAAGAC 2 cut(s) 533, 1242
BpmI CTGGAG 1 cut(s) 1544
Bpu10I CCTNAGC 1 cut(s) 171
Bpu1102I GCTNAGC 1 cut(s) 162
BpuEI CTTGAG 2 cut(s) 357, 370
BpuMI CCSGG 1 cut(s) 448
BsaBI GATNNNNATC 2 cut(s) 989, 1518
BsaHI GRCGYC 2 cut(s) 1953, 1995
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 9 cut(s) 32, 81, 176, 447, 1048, 1365, 1377, 1459, 1956
BsaWI WCCGGW 1 cut(s) 1114
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 1000, 1932
Bse1I ACTGG 5 cut(s) 630, 664, 823, 1004, 1953
Bse3DI GCAATG 2 cut(s) 1445, 1622
Bse8I GATNNNNATC 2 cut(s) 989, 1518
BseAI TCCGGA 1 cut(s) 1114
BseBI CCWGG 3 cut(s) 728, 1049, 1366
BseDI CCNNGG 9 cut(s) 32, 81, 176, 447, 1048, 1365, 1377, 1459, 1956
BseGI GGATG 7 cut(s) 122, 1111, 1373, 1502, 1648, 1912, 1984
BseJI GATNNNNATC 2 cut(s) 989, 1518
BseLI CCNNNNNNNGG 3 cut(s) 33, 1000, 1932
BseMI GCAATG 2 cut(s) 1445, 1622
BseMII CTCAG 7 cut(s) 153, 185, 248, 545, 918, 1277, 1974
BseNI ACTGG 5 cut(s) 630, 664, 823, 1004, 1953
BseRI GAGGAG 4 cut(s) 88, 91, 118, 1789
BseSI GKGCMC 2 cut(s) 603, 676
BshFI GGCC 2 cut(s) 275, 1021
BshNI GGYRCC 2 cut(s) 515, 619
BsiHKAI GWGCWC 3 cut(s) 168, 603, 676
BsiHKCI CYCGRG 1 cut(s) 1106
BsiSI CCGG 3 cut(s) 447, 1115, 1701
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 3 cut(s) 33, 1000, 1932
BsmAI GTCTC 6 cut(s) 197, 550, 634, 672, 998, 1835
BsmFI GGGAC 1 cut(s) 1065
BsnI GGCC 2 cut(s) 275, 1021
Bso31I GGTCTC 1 cut(s) 634
BsoBI CYCGRG 1 cut(s) 1106
Bsp1286I GDGCHC 3 cut(s) 168, 603, 676
Bsp13I TCCGGA 1 cut(s) 1114
Bsp1407I TGTACA 1 cut(s) 1567
Bsp1720I GCTNAGC 1 cut(s) 162
Bsp19I CCATGG 1 cut(s) 1956
BspACI CCGC 6 cut(s) 8, 24, 44, 47, 343, 1134
BspANI GGCC 2 cut(s) 275, 1021
BspCNI CTCAG 7 cut(s) 154, 184, 247, 546, 917, 1278, 1975
BspEI TCCGGA 1 cut(s) 1114
BspHI TCATGA 3 cut(s) 1545, 1732, 2030
BspLI GGNNCC 6 cut(s) 94, 517, 621, 1302, 1371, 1632
BspPI GGATC 8 cut(s) 291, 489, 646, 1136, 1364, 1377, 1627, 1711
BspT107I GGYRCC 2 cut(s) 515, 619
BspTI CTTAAG 1 cut(s) 269
BspTNI GGTCTC 1 cut(s) 634
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 1445, 1622
BsrGI TGTACA 1 cut(s) 1567
BsrI ACTGG 5 cut(s) 630, 664, 823, 1004, 1953
BssECI CCNNGG 9 cut(s) 32, 81, 176, 447, 1048, 1365, 1377, 1459, 1956
BssNAI GTATAC 1 cut(s) 1607
BssNI GRCGYC 2 cut(s) 1953, 1995
BssSI CACGAG 2 cut(s) 669, 936
BssT1I CCWWGG 5 cut(s) 32, 81, 1377, 1459, 1956
Bst1107I GTATAC 1 cut(s) 1607
Bst2BI CACGAG 2 cut(s) 669, 936
Bst2UI CCWGG 3 cut(s) 728, 1049, 1366
Bst4CI ACNGT 7 cut(s) 302, 771, 1101, 1160, 1385, 1572, 2003
Bst6I CTCTTC 2 cut(s) 227, 1931
BstACI GRCGYC 2 cut(s) 1953, 1995
BstAFI CTTAAG 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 1567
BstC8I GCNNGC 4 cut(s) 354, 1172, 1476, 1555
BstDEI CTNAG 8 cut(s) 162, 171, 234, 554, 904, 1185, 1286, 1983
BstDSI CCRYGG 1 cut(s) 1956
BstF5I GGATG 7 cut(s) 122, 1111, 1373, 1502, 1648, 1912, 1984
BstMAI GTCTC 6 cut(s) 197, 550, 634, 672, 998, 1835
BstMWI GCNNNNNNNGC 9 cut(s) 43, 46, 55, 172, 340, 349, 452, 1787, 2015
BstNI CCWGG 3 cut(s) 728, 1049, 1366
BstNSI RCATGY 3 cut(s) 1083, 1283, 1478
BstSCI CCNGG 4 cut(s) 446, 726, 1047, 1364
BstSFI CTRYAG 1 cut(s) 677
BstSLI GKGCMC 2 cut(s) 603, 676
BstV2I GAAGAC 2 cut(s) 533, 1242
BstX2I RGATCY 4 cut(s) 283, 638, 1369, 1703
BstXI CCANNNNNNTGG 2 cut(s) 1029, 1963
BstYI RGATCY 4 cut(s) 283, 638, 1369, 1703
BstZ17I GTATAC 1 cut(s) 1607
BsuRI GGCC 2 cut(s) 275, 1021
BtgI CCRYGG 1 cut(s) 1956
BtgZI GCGATG 1 cut(s) 1578
BtsCI GGATG 7 cut(s) 122, 1111, 1373, 1502, 1648, 1912, 1984
BtsI GCAGTG 1 cut(s) 2013
BtsIMutI CAGTG 6 cut(s) 307, 623, 1106, 1634, 1999, 2013
Cac8I GCNNGC 4 cut(s) 354, 1172, 1476, 1555
CaiI CAGNNNCTG 1 cut(s) 316
CciI TCATGA 3 cut(s) 1545, 1732, 2030
Cfr13I GGNCC 4 cut(s) 424, 730, 1019, 1300
CseI GACGC 1 cut(s) 1961
Csp6I GTAC 6 cut(s) 401, 609, 1062, 1568, 1587, 1771
CviQI GTAC 6 cut(s) 401, 609, 1062, 1568, 1587, 1771
DdeI CTNAG 8 cut(s) 162, 171, 234, 554, 904, 1185, 1286, 1983
DraI TTTAAA 1 cut(s) 1767
DraIII CACNNNGTG 1 cut(s) 598
DrdI GACNNNNNNGTC 2 cut(s) 422, 664
DseDI GACNNNNNNGTC 2 cut(s) 422, 664
Eam1104I CTCTTC 2 cut(s) 227, 1931
EarI CTCTTC 2 cut(s) 227, 1931
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 5 cut(s) 32, 81, 1377, 1459, 1956
Eco24I GRGCYC 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 634
Eco47I GGWCC 3 cut(s) 424, 730, 1300
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 5 cut(s) 208, 522, 1530, 1658, 1798
Eco88I CYCGRG 1 cut(s) 1106
EcoICRI GAGCTC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 1093
EcoRII CCWGG 3 cut(s) 726, 1047, 1364
EcoT14I CCWWGG 5 cut(s) 32, 81, 1377, 1459, 1956
EcoT22I ATGCAT 1 cut(s) 1502
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 5 cut(s) 32, 81, 1377, 1459, 1956
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 1 cut(s) 1065
FauI CCCGC 1 cut(s) 336
FauNDI CATATG 2 cut(s) 605, 652
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 1 cut(s) 1606
FokI GGATG 7 cut(s) 109, 1098, 1360, 1509, 1655, 1919, 1991
FriOI GRGCYC 1 cut(s) 168
FspBI CTAG 6 cut(s) 432, 497, 545, 860, 1124, 1590
GsuI CTGGAG 1 cut(s) 1544
HaeIII GGCC 2 cut(s) 275, 1021
HapII CCGG 3 cut(s) 447, 1115, 1701
HgaI GACGC 1 cut(s) 1961
Hin1I GRCGYC 2 cut(s) 1953, 1995
HincII GTYRAC 2 cut(s) 88, 794
HindII GTYRAC 2 cut(s) 88, 794
HindIII AAGCTT 2 cut(s) 1551, 1613
HinfI GANTC 5 cut(s) 181, 219, 416, 1068, 1288
HpaI GTTAAC 1 cut(s) 88
HpaII CCGG 3 cut(s) 447, 1115, 1701
HphI GGTGA 3 cut(s) 715, 1148, 1354
Hpy166II GTNNAC 8 cut(s) 88, 601, 674, 794, 924, 1225, 1607, 1999
Hpy8I GTNNAC 8 cut(s) 88, 601, 674, 794, 924, 1225, 1607, 1999
Hpy99I CGWCG 2 cut(s) 112, 118
HpyCH4III ACNGT 7 cut(s) 302, 771, 1101, 1160, 1385, 1572, 2003
HpyCH4IV ACGT 3 cut(s) 210, 1858, 1995
HpyF10VI GCNNNNNNNGC 9 cut(s) 43, 46, 55, 172, 340, 349, 452, 1787, 2015
HpyF3I CTNAG 8 cut(s) 162, 171, 234, 554, 904, 1185, 1286, 1983
HpySE526I ACGT 3 cut(s) 210, 1858, 1995
Hsp92I GRCGYC 2 cut(s) 1953, 1995
Kpn2I TCCGGA 1 cut(s) 1114
Ksp22I TGATCA 1 cut(s) 15
KspAI GTTAAC 1 cut(s) 88
LmnI GCTCC 7 cut(s) 83, 92, 171, 340, 1471, 1775, 1786
LweI GCATC 8 cut(s) 271, 464, 523, 656, 1120, 1487, 1663, 1969
MaeI CTAG 6 cut(s) 432, 497, 545, 860, 1124, 1590
MaeII ACGT 3 cut(s) 210, 1858, 1995
MaeIII GTNAC 4 cut(s) 655, 841, 1188, 1991
MbiI CCGCTC 1 cut(s) 343
MboII GAAGA 9 cut(s) 214, 250, 538, 1140, 1242, 1382, 1424, 1523, 1948
MfeI CAATTG 1 cut(s) 1785
MflI RGATCY 4 cut(s) 283, 638, 1369, 1703
MhlI GDGCHC 3 cut(s) 168, 603, 676
MlyI GAGTC 3 cut(s) 213, 410, 1297
MmeI TCCRAC 4 cut(s) 318, 343, 616, 1278
Mph1103I ATGCAT 1 cut(s) 1502
MroI TCCGGA 1 cut(s) 1114
MseI TTAA 8 cut(s) 87, 270, 848, 1200, 1310, 1757, 1766, 1793
MslI CAYNNNNRTG 4 cut(s) 434, 1580, 1836, 1961
MspA1I CMGCKG 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 269
MspI CCGG 3 cut(s) 447, 1115, 1701
MspR9I CCNGG 4 cut(s) 448, 728, 1049, 1366
MunI CAATTG 1 cut(s) 1785
MvaI CCWGG 3 cut(s) 728, 1049, 1366
MwoI GCNNNNNNNGC 9 cut(s) 43, 46, 55, 172, 340, 349, 452, 1787, 2015
NciI CCSGG 1 cut(s) 448
NcoI CCATGG 1 cut(s) 1956
NdeI CATATG 2 cut(s) 605, 652
NlaIV GGNNCC 6 cut(s) 94, 517, 621, 1302, 1371, 1632
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 3 cut(s) 655, 1188, 1991
NsiI ATGCAT 1 cut(s) 1502
NspI RCATGY 3 cut(s) 1083, 1283, 1478
PaeI GCATGC 1 cut(s) 1478
PaeR7I CTCGAG 1 cut(s) 1106
PagI TCATGA 3 cut(s) 1545, 1732, 2030
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 116
PfeI GAWTC 2 cut(s) 181, 1068
PflMI CCANNNNNTGG 1 cut(s) 33
PleI GAGTC 3 cut(s) 213, 410, 1296
PpsI GAGTC 3 cut(s) 213, 410, 1296
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 3 cut(s) 726, 1047, 1364
PspGI CCWGG 3 cut(s) 726, 1047, 1364
PspN4I GGNNCC 6 cut(s) 94, 517, 621, 1302, 1371, 1632
PspPI GGNCC 4 cut(s) 424, 730, 1019, 1300
PspXI VCTCGAGB 1 cut(s) 1106
PstNI CAGNNNCTG 1 cut(s) 316
PsuI RGATCY 4 cut(s) 283, 638, 1369, 1703
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 6 cut(s) 402, 610, 1063, 1569, 1588, 1772
RsaNI GTAC 6 cut(s) 401, 609, 1062, 1568, 1587, 1771
RseI CAYNNNNRTG 4 cut(s) 434, 1580, 1836, 1961
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 8 cut(s) 87, 270, 848, 1200, 1310, 1757, 1766, 1793
Sau96I GGNCC 4 cut(s) 424, 730, 1019, 1300
ScaI AGTACT 1 cut(s) 402
SchI GAGTC 3 cut(s) 213, 410, 1297
ScrFI CCNGG 4 cut(s) 448, 728, 1049, 1366
SduI GDGCHC 3 cut(s) 168, 603, 676
SfaNI GCATC 8 cut(s) 271, 464, 523, 656, 1120, 1487, 1663, 1969
SfcI CTRYAG 1 cut(s) 677
Sfr274I CTCGAG 1 cut(s) 1106
SinI GGWCC 3 cut(s) 424, 730, 1300
SlaI CTCGAG 1 cut(s) 1106
SmiMI CAYNNNNRTG 4 cut(s) 434, 1580, 1836, 1961
SmlI CTYRAG 4 cut(s) 269, 372, 385, 1106
SmoI CTYRAG 4 cut(s) 269, 372, 385, 1106
SphI GCATGC 1 cut(s) 1478
SsiI CCGC 6 cut(s) 8, 24, 44, 47, 343, 1134
SspI AATATT 2 cut(s) 244, 1204
SspMI CTAG 6 cut(s) 432, 497, 545, 860, 1124, 1590
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 4 cut(s) 446, 726, 1047, 1364
StyI CCWWGG 5 cut(s) 32, 81, 1377, 1459, 1956
TaaI ACNGT 7 cut(s) 302, 771, 1101, 1160, 1385, 1572, 2003
TaiI ACGT 3 cut(s) 213, 1861, 1998
TaqI TCGA 3 cut(s) 1107, 1410, 1928
TatI WGTACW 2 cut(s) 400, 1567
TauI GCSGC 2 cut(s) 11, 49
TfiI GAWTC 2 cut(s) 181, 1068
Tru1I TTAA 8 cut(s) 87, 270, 848, 1200, 1310, 1757, 1766, 1793
Tru9I TTAA 8 cut(s) 87, 270, 848, 1200, 1310, 1757, 1766, 1793
TscAI CASTG 6 cut(s) 307, 630, 1106, 1641, 2006, 2020
TseFI GTSAC 3 cut(s) 655, 1188, 1991
Tsp45I GTSAC 3 cut(s) 655, 1188, 1991
TspGWI ACGGA 3 cut(s) 105, 626, 1788
TspRI CASTG 6 cut(s) 307, 630, 1106, 1641, 2006, 2020
Van91I CCANNNNNTGG 1 cut(s) 33
Vha464I CTTAAG 1 cut(s) 269
VneI GTGCAC 2 cut(s) 599, 672
VpaK11BI GGWCC 3 cut(s) 424, 730, 1300
XapI RAATTY 5 cut(s) 741, 810, 1093, 1399, 1655
XbaI TCTAGA 1 cut(s) 859
XceI RCATGY 3 cut(s) 1083, 1283, 1478
XhoI CTCGAG 1 cut(s) 1106
XmiI GTMKAC 1 cut(s) 1606
XspI CTAG 6 cut(s) 432, 497, 545, 860, 1124, 1590
ZraI GACGTC 1 cut(s) 1996
ZrmI AGTACT 1 cut(s) 402
Zsp2I ATGCAT 1 cut(s) 1502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.