Rh2CG216400

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018725
Physical Location & Seq
Reverse (-)
21276308 .. 21282760
6453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG216400.1

Sequence Viewer

Length: 1890 bp
ATGAAGGCGGCGTTGATCAGCACCGCCATTCCTTGGCAGCCTCCGCCGCACCATAAGCCTCTGAAACCCCTTTGTTGCTCCAAGGTTAACGGAGCCTCCTCCTCGCCGTCGCCGTCGCCGTCATCCTCCTCACAAATCAAGTTGCAAGGCAGCGAAAATGCTGAGCTCCCTCAGCCTCGGATTCAGTCTGGGGAAGCGAAGGAGACTGACGTTTGGCGACTCTTCAGAGAAGCTCAAAAGAATATTCTGCATTTGAACCAGCATCGGCTTAAGGCCGTAGAGGATCTGAACAAAACAAACAGTGAGAAGCAGCTGCTGATTGATAAGATTGAGCAGTTGGAGCGGGAAAAGCAGGCTTCTGTTGGAAAGCCTCAAGATAGGCTCTCAAGTTGTTGGGAAGTACTCCTTCGGATAGACTCAATGGTCCTCACTAGAATGATTGCTGCCGGGGAGGCATCTGATTTGAGAAGGCTGGTCACGGATCATAAAATGAGTCTAGCTGAAGTTTTTGATGGCACCTTGCAGAAGACAGATGCCGAGCTTCTAGCTGAACTGAGACACTTCTTTGCAAGAAGCAGAAGGAATGGCTTTCACATTGTGCACATATGTACGGAAATGGCACCACTGGTCTCTGTTGGATCTTTGGCATCATATGTGACTGGCTTGTCTCGTGCACTACAGAGGAAGGGGCATTTGGTGGAGGTGATACTTCCAAAGTATTCAAGCCTGGACCTAGATGAAATTCAAGGGCTACAAGAGATTGAGGCAGAGTCCTTTTCATATTTCAATGGTCAACTTCATGGAAACAGAATTTGGACTGGTGTTGTCTATGGCATTGGAGTTACCTTAATTCAACCTCTAGAGTATTCAGAGTTTTTCAATCGTGAAAAGGTGTATGGCTACTCAGATGACTTTGAAAGGTTTACCTATTTCTCTCGTGCTTCATTAGATTATATTGTAAAGTGTGGAAAGCAACCTGATGTGATACATATCCACAACTGGGAGACTGCTATTATTGGGCCACTTTTCTGGGATATTTTTGCTAACCAGGGACTTGAAGGTACGAGAATCTTATTGACATGCCATGACCTGAATTCACAGTGCCTCGAGCATCCGGATAAACTAGCGTTATGCGGACTTGATCCTTCTTCACTTCACCGTCCTGATCGCTTGCAAGATAATACTAAGTCACATTTGGTTAATATTTTGAAGGGTGGAGTTGTTTACTCCAATAATGTTGTCATAATGTCCTCCGTACTATCAAAAGGCAGGGTTATACATACTCTGAGTCATGGGTTGGACCCTACCTTAAACATTCACAAGAACAAGTTGGTTGTTTCTCCTTGTGGGTTTGACAATTCCGCCTGGGATCCATCCAAGGACAGCTTTCTTCCCCGAAATTTCAATGTCGAAGATATGGAAGGGAAAGCAGTTTGCAAAGCTGCATTGCAGCAGCACCTTGGGTTATCGGAGCATGCTTCTACCATTCTTATTGGATGCATTGTGTCTGAAGATGTTGATCTGGAGAAGATGAGGGCAGTAATCATGAAAGCTTGCAAGAGCGATGTACAGTTCATCATCATGGGTACTAGCAAATTATCAAGTATACACAATCTTGGATCATTGCAGGAAACACTGAAGGATGAACAAGTGAAATTCGTAAATGAGTGTGATGCAGCAATAACACATTTGGTTTTTGCCGGATCTGATATTATCTTGTGCCAATCTTTTCATGACCCTGTTCTCCAAGTTCCAGTTAGCTCTCTCTCTGTCTCTTTCTTAGCTTTATATCATCACTTATTTGATGTCCATACTCATTTTTATCCAAATTATGTAGTTAAAGGCTTTAAAGTACGGAGCTGCTCCAATTGCATTAAACTCCTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

629

Amino Acids

70.34

Weight (kDa)

6.64

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 198 - 422 9e-40 Starch synthase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66595
rosa_chinensis RchiOBHm_Chr2g0109671
rosa_multiflora Rmu_sc0000332.1_g000062 Rmu_sc0027285.1_g000001
rosa_rugosa Rorug02G0161800
rosa_samantha Rh2AG214100 Rh2BG224400 Rh2CG216400 Rh2DG219700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 422, 664
AccB1I GGYRCC 2 cut(s) 515, 619
AccB7I CCANNNNNTGG 1 cut(s) 33
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 1606
AccIII TCCGGA 1 cut(s) 1114
AciI CCGC 7 cut(s) 8, 24, 44, 47, 343, 1134, 1362
AclWI GGATC 8 cut(s) 291, 489, 646, 1136, 1364, 1377, 1627, 1711
AcsI RAATTY 5 cut(s) 741, 810, 1093, 1399, 1655
AcuI CTGAAG 4 cut(s) 208, 522, 1530, 1658
AdeI CACNNNGTG 1 cut(s) 598
AfaI GTAC 7 cut(s) 402, 610, 1063, 1257, 1569, 1588, 1854
AfiI CCNNNNNNNGG 2 cut(s) 33, 1000
AflII CTTAAG 1 cut(s) 269
AjnI CCWGG 3 cut(s) 726, 1047, 1364
AjuI GAANNNNNNNTTGG 4 cut(s) 677, 709, 796, 828
Alw21I GWGCWC 3 cut(s) 168, 603, 676
Alw26I GTCTC 6 cut(s) 197, 550, 634, 672, 998, 1777
Alw44I GTGCAC 2 cut(s) 599, 672
AlwI GGATC 8 cut(s) 291, 489, 646, 1136, 1364, 1377, 1627, 1711
AlwNI CAGNNNCTG 1 cut(s) 316
Ama87I CYCGRG 1 cut(s) 1106
Aor13HI TCCGGA 1 cut(s) 1114
AoxI GGCC 2 cut(s) 273, 1019
ApaLI GTGCAC 2 cut(s) 599, 672
ApoI RAATTY 5 cut(s) 741, 810, 1093, 1399, 1655
AspS9I GGNCC 4 cut(s) 424, 730, 1019, 1300
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 2 cut(s) 715, 1148
AvaI CYCGRG 1 cut(s) 1106
AvaII GGWCC 3 cut(s) 424, 730, 1300
BaeGI GKGCMC 2 cut(s) 603, 676
BamHI GGATCC 1 cut(s) 1369
BanI GGYRCC 2 cut(s) 515, 619
BanII GRGCYC 1 cut(s) 168
BauI CACGAG 2 cut(s) 669, 936
BbsI GAAGAC 1 cut(s) 533
Bbv12I GWGCWC 3 cut(s) 168, 603, 676
BbvCI CCTCAGC 1 cut(s) 171
BccI CCATC 2 cut(s) 506, 1381
BceAI ACGGC 4 cut(s) 91, 97, 103, 260
BciT130I CCWGG 3 cut(s) 728, 1049, 1366
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 6 cut(s) 197, 550, 634, 672, 998, 1777
BfaI CTAG 7 cut(s) 432, 497, 545, 734, 860, 1124, 1590
BfmI CTRYAG 1 cut(s) 677
BfrI CTTAAG 1 cut(s) 269
BglI GCCNNNNNGGC 1 cut(s) 452
BlpI GCTNAGC 1 cut(s) 162
BmcAI AGTACT 1 cut(s) 402
Bme1390I CCNGG 4 cut(s) 448, 728, 1049, 1366
Bme18I GGWCC 3 cut(s) 424, 730, 1300
BmeT110I CYCGRG 1 cut(s) 1106
BmgT120I GGNCC 4 cut(s) 424, 730, 1019, 1300
BmiI GGNNCC 5 cut(s) 94, 517, 621, 1302, 1371
BmrFI CCNGG 4 cut(s) 448, 728, 1049, 1366
BmrI ACTGGG 1 cut(s) 1009
BmsI GCATC 7 cut(s) 271, 464, 523, 656, 1120, 1487, 1663
BmuI ACTGGG 1 cut(s) 1009
BpiI GAAGAC 1 cut(s) 533
BpmI CTGGAG 1 cut(s) 1544
Bpu10I CCTNAGC 1 cut(s) 171
Bpu1102I GCTNAGC 1 cut(s) 162
BpuEI CTTGAG 2 cut(s) 357, 370
BpuMI CCSGG 1 cut(s) 448
BsaBI GATNNNNATC 2 cut(s) 989, 1518
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 8 cut(s) 32, 81, 176, 447, 1048, 1365, 1377, 1459
BsaWI WCCGGW 1 cut(s) 1114
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 1000
Bse1I ACTGG 5 cut(s) 630, 664, 823, 1004, 1754
Bse3DI GCAATG 2 cut(s) 1445, 1622
Bse8I GATNNNNATC 2 cut(s) 989, 1518
BseAI TCCGGA 1 cut(s) 1114
BseBI CCWGG 3 cut(s) 728, 1049, 1366
BseDI CCNNGG 8 cut(s) 32, 81, 176, 447, 1048, 1365, 1377, 1459
BseGI GGATG 5 cut(s) 122, 1111, 1373, 1502, 1648
BseJI GATNNNNATC 2 cut(s) 989, 1518
BseLI CCNNNNNNNGG 2 cut(s) 33, 1000
BseMI GCAATG 2 cut(s) 1445, 1622
BseMII CTCAG 5 cut(s) 153, 185, 545, 918, 1277
BseNI ACTGG 5 cut(s) 630, 664, 823, 1004, 1754
BseRI GAGGAG 4 cut(s) 88, 91, 118, 1871
BseSI GKGCMC 2 cut(s) 603, 676
BshFI GGCC 2 cut(s) 275, 1021
BshNI GGYRCC 2 cut(s) 515, 619
BsiHKAI GWGCWC 3 cut(s) 168, 603, 676
BsiHKCI CYCGRG 1 cut(s) 1106
BsiSI CCGG 3 cut(s) 447, 1115, 1701
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 2 cut(s) 33, 1000
BsmAI GTCTC 6 cut(s) 197, 550, 634, 672, 998, 1777
BsmFI GGGAC 1 cut(s) 1065
BsnI GGCC 2 cut(s) 275, 1021
Bso31I GGTCTC 1 cut(s) 634
BsoBI CYCGRG 1 cut(s) 1106
Bsp1286I GDGCHC 3 cut(s) 168, 603, 676
Bsp13I TCCGGA 1 cut(s) 1114
Bsp1407I TGTACA 1 cut(s) 1567
Bsp1720I GCTNAGC 1 cut(s) 162
BspACI CCGC 7 cut(s) 8, 24, 44, 47, 343, 1134, 1362
BspANI GGCC 2 cut(s) 275, 1021
BspCNI CTCAG 5 cut(s) 154, 184, 546, 917, 1278
BspEI TCCGGA 1 cut(s) 1114
BspHI TCATGA 2 cut(s) 1545, 1732
BspLI GGNNCC 5 cut(s) 94, 517, 621, 1302, 1371
BspPI GGATC 8 cut(s) 291, 489, 646, 1136, 1364, 1377, 1627, 1711
BspT107I GGYRCC 2 cut(s) 515, 619
BspTI CTTAAG 1 cut(s) 269
BspTNI GGTCTC 1 cut(s) 634
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 1445, 1622
BsrGI TGTACA 1 cut(s) 1567
BsrI ACTGG 5 cut(s) 630, 664, 823, 1004, 1754
BssECI CCNNGG 8 cut(s) 32, 81, 176, 447, 1048, 1365, 1377, 1459
BssNAI GTATAC 1 cut(s) 1607
BssSI CACGAG 2 cut(s) 669, 936
BssT1I CCWWGG 4 cut(s) 32, 81, 1377, 1459
Bst1107I GTATAC 1 cut(s) 1607
Bst2BI CACGAG 2 cut(s) 669, 936
Bst2UI CCWGG 3 cut(s) 728, 1049, 1366
Bst4CI ACNGT 4 cut(s) 302, 1101, 1160, 1572
Bst6I CTCTTC 1 cut(s) 227
BstAFI CTTAAG 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 1567
BstC8I GCNNGC 4 cut(s) 354, 1172, 1476, 1555
BstDEI CTNAG 7 cut(s) 162, 171, 554, 904, 1185, 1286, 1780
BstF5I GGATG 5 cut(s) 122, 1111, 1373, 1502, 1648
BstMAI GTCTC 6 cut(s) 197, 550, 634, 672, 998, 1777
BstMWI GCNNNNNNNGC 8 cut(s) 43, 46, 55, 172, 340, 349, 452, 1869
BstNI CCWGG 3 cut(s) 728, 1049, 1366
BstNSI RCATGY 2 cut(s) 1083, 1478
BstSCI CCNGG 4 cut(s) 446, 726, 1047, 1364
BstSFI CTRYAG 1 cut(s) 677
BstSLI GKGCMC 2 cut(s) 603, 676
BstV2I GAAGAC 1 cut(s) 533
BstX2I RGATCY 4 cut(s) 283, 638, 1369, 1703
BstXI CCANNNNNNTGG 1 cut(s) 1029
BstYI RGATCY 4 cut(s) 283, 638, 1369, 1703
BstZ17I GTATAC 1 cut(s) 1607
BsuRI GGCC 2 cut(s) 275, 1021
BtgZI GCGATG 1 cut(s) 1578
BtsCI GGATG 5 cut(s) 122, 1111, 1373, 1502, 1648
BtsIMutI CAGTG 4 cut(s) 307, 623, 1106, 1634
Cac8I GCNNGC 4 cut(s) 354, 1172, 1476, 1555
CaiI CAGNNNCTG 1 cut(s) 316
CciI TCATGA 2 cut(s) 1545, 1732
Cfr13I GGNCC 4 cut(s) 424, 730, 1019, 1300
Csp6I GTAC 7 cut(s) 401, 609, 1062, 1256, 1568, 1587, 1853
CviAII CATG 9 cut(s) 800, 1080, 1085, 1292, 1475, 1546, 1582, 1733, 1887
CviQI GTAC 7 cut(s) 401, 609, 1062, 1256, 1568, 1587, 1853
DdeI CTNAG 7 cut(s) 162, 171, 554, 904, 1185, 1286, 1780
DraI TTTAAA 1 cut(s) 1849
DraIII CACNNNGTG 1 cut(s) 598
DrdI GACNNNNNNGTC 2 cut(s) 422, 664
DseDI GACNNNNNNGTC 2 cut(s) 422, 664
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
EciI GGCGGA 2 cut(s) 33, 1351
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 4 cut(s) 32, 81, 1377, 1459
Eco24I GRGCYC 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 634
Eco47I GGWCC 3 cut(s) 424, 730, 1300
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 4 cut(s) 208, 522, 1530, 1658
Eco88I CYCGRG 1 cut(s) 1106
EcoICRI GAGCTC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 1093
EcoRII CCWGG 3 cut(s) 726, 1047, 1364
EcoT14I CCWWGG 4 cut(s) 32, 81, 1377, 1459
EcoT22I ATGCAT 1 cut(s) 1502
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 4 cut(s) 32, 81, 1377, 1459
FaeI CATG 9 cut(s) 803, 1083, 1088, 1295, 1478, 1549, 1585, 1736, 1890
FalI AAGNNNNNCTT 4 cut(s) 390, 422, 1370, 1402
FaqI GGGAC 1 cut(s) 1065
FatI CATG 9 cut(s) 799, 1079, 1084, 1291, 1474, 1545, 1581, 1732, 1886
FauI CCCGC 1 cut(s) 336
FauNDI CATATG 2 cut(s) 605, 652
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 1 cut(s) 1606
FokI GGATG 5 cut(s) 109, 1098, 1360, 1509, 1655
FriOI GRGCYC 1 cut(s) 168
FspBI CTAG 7 cut(s) 432, 497, 545, 734, 860, 1124, 1590
GsuI CTGGAG 1 cut(s) 1544
HaeIII GGCC 2 cut(s) 275, 1021
HapII CCGG 3 cut(s) 447, 1115, 1701
Hin1II CATG 9 cut(s) 803, 1083, 1088, 1295, 1478, 1549, 1585, 1736, 1890
HincII GTYRAC 2 cut(s) 88, 794
HindII GTYRAC 2 cut(s) 88, 794
HindIII AAGCTT 1 cut(s) 1551
HinfI GANTC 7 cut(s) 181, 219, 416, 493, 770, 1068, 1288
HpaI GTTAAC 1 cut(s) 88
HpaII CCGG 3 cut(s) 447, 1115, 1701
HphI GGTGA 2 cut(s) 715, 1148
Hpy166II GTNNAC 7 cut(s) 88, 601, 674, 794, 924, 1225, 1607
Hpy188III TCNNGA 8 cut(s) 374, 860, 884, 1115, 1163, 1523, 1546, 1733
Hpy8I GTNNAC 7 cut(s) 88, 601, 674, 794, 924, 1225, 1607
Hpy99I CGWCG 2 cut(s) 112, 118
HpyCH4III ACNGT 4 cut(s) 302, 1101, 1160, 1572
HpyCH4IV ACGT 1 cut(s) 210
HpyF10VI GCNNNNNNNGC 8 cut(s) 43, 46, 55, 172, 340, 349, 452, 1869
HpyF3I CTNAG 7 cut(s) 162, 171, 554, 904, 1185, 1286, 1780
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 9 cut(s) 803, 1083, 1088, 1295, 1478, 1549, 1585, 1736, 1890
Kpn2I TCCGGA 1 cut(s) 1114
Ksp22I TGATCA 1 cut(s) 15
KspAI GTTAAC 1 cut(s) 88
LmnI GCTCC 7 cut(s) 83, 92, 171, 340, 1471, 1857, 1868
LweI GCATC 7 cut(s) 271, 464, 523, 656, 1120, 1487, 1663
MaeI CTAG 7 cut(s) 432, 497, 545, 734, 860, 1124, 1590
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 4 cut(s) 475, 655, 841, 1188
MbiI CCGCTC 1 cut(s) 343
MboII GAAGA 7 cut(s) 214, 538, 1140, 1382, 1424, 1523, 1540
MfeI CAATTG 1 cut(s) 1867
MflI RGATCY 4 cut(s) 283, 638, 1369, 1703
MhlI GDGCHC 3 cut(s) 168, 603, 676
MlyI GAGTC 5 cut(s) 213, 410, 502, 779, 1297
MmeI TCCRAC 4 cut(s) 318, 343, 616, 1278
Mph1103I ATGCAT 1 cut(s) 1502
MroI TCCGGA 1 cut(s) 1114
MseI TTAA 8 cut(s) 87, 270, 848, 1200, 1310, 1839, 1848, 1875
MslI CAYNNNNRTG 2 cut(s) 434, 1580
MspA1I CMGCKG 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 269
MspI CCGG 3 cut(s) 447, 1115, 1701
MspR9I CCNGG 4 cut(s) 448, 728, 1049, 1366
MunI CAATTG 1 cut(s) 1867
MvaI CCWGG 3 cut(s) 728, 1049, 1366
MwoI GCNNNNNNNGC 8 cut(s) 43, 46, 55, 172, 340, 349, 452, 1869
NciI CCSGG 1 cut(s) 448
NdeI CATATG 2 cut(s) 605, 652
NlaIII CATG 9 cut(s) 803, 1083, 1088, 1295, 1478, 1549, 1585, 1736, 1890
NlaIV GGNNCC 5 cut(s) 94, 517, 621, 1302, 1371
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 3 cut(s) 475, 655, 1188
NsiI ATGCAT 1 cut(s) 1502
NspI RCATGY 2 cut(s) 1083, 1478
PaeI GCATGC 1 cut(s) 1478
PaeR7I CTCGAG 1 cut(s) 1106
PagI TCATGA 2 cut(s) 1545, 1732
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 116
PfeI GAWTC 2 cut(s) 181, 1068
PflMI CCANNNNNTGG 1 cut(s) 33
PleI GAGTC 5 cut(s) 213, 410, 501, 778, 1296
PpsI GAGTC 5 cut(s) 213, 410, 501, 778, 1296
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 3 cut(s) 726, 1047, 1364
PspGI CCWGG 3 cut(s) 726, 1047, 1364
PspN4I GGNNCC 5 cut(s) 94, 517, 621, 1302, 1371
PspPI GGNCC 4 cut(s) 424, 730, 1019, 1300
PspXI VCTCGAGB 1 cut(s) 1106
PstNI CAGNNNCTG 1 cut(s) 316
PsuI RGATCY 4 cut(s) 283, 638, 1369, 1703
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 7 cut(s) 402, 610, 1063, 1257, 1569, 1588, 1854
RsaNI GTAC 7 cut(s) 401, 609, 1062, 1256, 1568, 1587, 1853
RseI CAYNNNNRTG 2 cut(s) 434, 1580
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 8 cut(s) 87, 270, 848, 1200, 1310, 1839, 1848, 1875
Sau96I GGNCC 4 cut(s) 424, 730, 1019, 1300
ScaI AGTACT 1 cut(s) 402
SchI GAGTC 5 cut(s) 213, 410, 502, 779, 1297
ScrFI CCNGG 4 cut(s) 448, 728, 1049, 1366
SduI GDGCHC 3 cut(s) 168, 603, 676
SfaNI GCATC 7 cut(s) 271, 464, 523, 656, 1120, 1487, 1663
SfcI CTRYAG 1 cut(s) 677
Sfr274I CTCGAG 1 cut(s) 1106
SinI GGWCC 3 cut(s) 424, 730, 1300
SlaI CTCGAG 1 cut(s) 1106
SmiMI CAYNNNNRTG 2 cut(s) 434, 1580
SmlI CTYRAG 4 cut(s) 269, 372, 385, 1106
SmoI CTYRAG 4 cut(s) 269, 372, 385, 1106
SphI GCATGC 1 cut(s) 1478
SsiI CCGC 7 cut(s) 8, 24, 44, 47, 343, 1134, 1362
SspI AATATT 2 cut(s) 244, 1204
SspMI CTAG 7 cut(s) 432, 497, 545, 734, 860, 1124, 1590
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 4 cut(s) 446, 726, 1047, 1364
StyI CCWWGG 4 cut(s) 32, 81, 1377, 1459
TaaI ACNGT 4 cut(s) 302, 1101, 1160, 1572
TaiI ACGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 1107, 1410
TatI WGTACW 2 cut(s) 400, 1567
TauI GCSGC 2 cut(s) 11, 49
TfiI GAWTC 2 cut(s) 181, 1068
Tru1I TTAA 8 cut(s) 87, 270, 848, 1200, 1310, 1839, 1848, 1875
Tru9I TTAA 8 cut(s) 87, 270, 848, 1200, 1310, 1839, 1848, 1875
TscAI CASTG 4 cut(s) 307, 630, 1106, 1641
TseFI GTSAC 3 cut(s) 475, 655, 1188
Tsp45I GTSAC 3 cut(s) 475, 655, 1188
TspDTI ATGAA 9 cut(s) 17, 753, 768, 788, 933, 1562, 1564, 1659, 1721
TspGWI ACGGA 5 cut(s) 105, 494, 626, 1243, 1870
TspRI CASTG 4 cut(s) 307, 630, 1106, 1641
Van91I CCANNNNNTGG 1 cut(s) 33
Vha464I CTTAAG 1 cut(s) 269
VneI GTGCAC 2 cut(s) 599, 672
VpaK11BI GGWCC 3 cut(s) 424, 730, 1300
XapI RAATTY 5 cut(s) 741, 810, 1093, 1399, 1655
XbaI TCTAGA 1 cut(s) 859
XceI RCATGY 2 cut(s) 1083, 1478
XhoI CTCGAG 1 cut(s) 1106
XmiI GTMKAC 1 cut(s) 1606
XspI CTAG 7 cut(s) 432, 497, 545, 734, 860, 1124, 1590
ZrmI AGTACT 1 cut(s) 402
Zsp2I ATGCAT 1 cut(s) 1502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.