Rh2BG224400

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018725
Physical Location & Seq
Reverse (-)
21750962 .. 21757557
6596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG224400.1

Sequence Viewer

Length: 1884 bp
ATGAAGGCGGCGTTGATCAGCACCGCCATTCCTTGGCAGCCTCCGCCGCACCATAAGCCTCTGAAACCCCTTTGTTGCTCCAAGGTTAACGGAGCCTCCTCCTCGCCGTCGCCGTCATCCTCCTCACAAATCAAGTTGCAAGGCAGCGAAAATGCTGAGCTCCCTCAGCCTCGGATTCAGTCTGGGGAAGCGAAGGAGACTGACGTTTGGCGACTCTTCAGAGAAGCTCAGAAGAATATTCTGCATTTGAACCAGCATCGGCTTAAGGCCGTAGAGGATCTGAACAAAACAAACAGTGAGAAGCAGCTGCTGATTGATAGGATTGAGCAGTTGGAGCGGGAAAAGCAGGCTTCTGTTGGAAAGCCTCAAGATAGGCTCTCAAGTTGTTGGGAAGTACTCCTTCGGATAGACTCAATGGTCCTCACTAGAATGATTGCTGCTGGGGAGGCATCTGATTTGAGAAGGCTGGTCACAGATCACAAAATGAGTCTAGCTGAAGTTTTTGATGGCACCTTGCAGAAGACAGATGCCGAGCTTCTAGCTGAACTGAGACACTTCTTTGCAAGAAGCAGAAGGAATGGCTTTCACATTGTGCACATATGTACGGAAATGGCACCATTGGTCTCTGTTGGATCTTTGGCATCATATGTGACTGGCTTGTCTCGTGCACTACAGAGGAAGGGGCATTTGGTGGAGGTGATACTTCCAAAGTATTCAAGCCTGGACCTAGATGAAATTCAAGGGCTACAAGAGATTGAGGCAGAGTCCTTTTCATATTTCAATGGTCAACTTCATGGAAACAGAATTTGGACTGGTGTTGTCTATGGCATTGGAGTTACCTTAATTCAACCTCTAGAGTATTCAGAGTTTTTCAATCGTGAAAAGGTGTATGGCTACTCAGATGACTTTGAAAGGTTTACCTATTTCTCTCGTGCTTCATTAGATTATATTGTAAAGTGTGGAAAGCAACCTGATGTGATACATATCCACAACTGGGAGACTGCTATTATTGGGCCACTTTTCTGGGATATTTTTGCTAACCAGGGACTTGAAGGTACGAGAATCTTATTGACATGCCATGACCTGAATTCACAGTGCCTCGAGCATCCGGATAAACTAGCGTTATGCGGACTTGATCCTTCTTCACTTCACCGTCCTGATCGCTTGCAAGATAATACTAAGTCACATTTGGTTAATATTTTGAAGGGTGGAGTTGTTTACTCCAATAATGTTGTCATAATGTCCTCCATACTATCAAAAGGCAGGGTTATACATACTCTGAGTCATGGGTTGGACCCTACCTTAAACATTCACAAGAACAAGTTGGTTGTTTCTCCTTGTGGATTTGACAATTCCACCTGGGATCCATCCAAGGACAGATTTCTTCCCCGAAATTTCAATGTCGAAGATATGGAAGGGAAAGCAGTTTGCAAAGCTGCATTGCAGCAGCACCTTGGGTTATTGGAGCATGCTTCTACCATTCTTATTGGATGCATTGTGTCTGAAGATGTTGATCTGGAGAACATGAGGGCAGTAATCATGAAAGCTTGCAAGAGCGATGTACAGTTCATCATCATGGGTACTAGCAAATTATCAAGTATACACAAGCTTGGATCATTGCAGGAACCACTGAAGGATGAACAAGTGAAATTCGTAAATGAGTGTGATGCAGCAATAACACATTTGGTTTTTGCCGGATCTGATATTATCTTGTGCCAATCTTTTCATGACCCTGTTCTCCAAGTTCCAGTTAGCTCTCTCTCTGTCTCTTTCTTAGCTTTATATCATCACTTATTTGATGTCCATACTCATTTTTATCCAAATTATGTAGTTAAAGGCTTTAAAGTACGGAGCTGCTCCAATTGCATTAAACTCCTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

627

Amino Acids

70.32

Weight (kDa)

6.73

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 196 - 418 1.1e-39 Starch synthase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66595
rosa_chinensis RchiOBHm_Chr2g0109671
rosa_multiflora Rmu_sc0000332.1_g000062 Rmu_sc0027285.1_g000001
rosa_rugosa Rorug02G0161800
rosa_samantha Rh2AG214100 Rh2BG224400 Rh2CG216400 Rh2DG219700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 416, 658
AccB1I GGYRCC 2 cut(s) 509, 613
AccB7I CCANNNNNTGG 1 cut(s) 33
AccBSI CCGCTC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 1600
AccIII TCCGGA 1 cut(s) 1108
AciI CCGC 6 cut(s) 8, 24, 44, 47, 337, 1128
AclWI GGATC 7 cut(s) 285, 640, 1130, 1358, 1371, 1621, 1705
AcsI RAATTY 5 cut(s) 735, 804, 1087, 1393, 1649
AcuI CTGAAG 4 cut(s) 202, 516, 1524, 1652
AdeI CACNNNGTG 1 cut(s) 592
AfaI GTAC 6 cut(s) 396, 604, 1057, 1563, 1582, 1848
AfiI CCNNNNNNNGG 2 cut(s) 33, 994
AflII CTTAAG 1 cut(s) 263
AjnI CCWGG 3 cut(s) 720, 1041, 1358
AjuI GAANNNNNNNTTGG 4 cut(s) 671, 703, 790, 822
Alw21I GWGCWC 3 cut(s) 162, 597, 670
Alw26I GTCTC 6 cut(s) 191, 544, 628, 666, 992, 1771
Alw44I GTGCAC 2 cut(s) 593, 666
AlwI GGATC 7 cut(s) 285, 640, 1130, 1358, 1371, 1621, 1705
AlwNI CAGNNNCTG 1 cut(s) 310
Ama87I CYCGRG 1 cut(s) 1100
Aor13HI TCCGGA 1 cut(s) 1108
AoxI GGCC 2 cut(s) 267, 1013
ApaLI GTGCAC 2 cut(s) 593, 666
ApoI RAATTY 5 cut(s) 735, 804, 1087, 1393, 1649
AspS9I GGNCC 4 cut(s) 418, 724, 1013, 1294
AsuHPI GGTGA 2 cut(s) 709, 1142
AvaI CYCGRG 1 cut(s) 1100
AvaII GGWCC 3 cut(s) 418, 724, 1294
BaeGI GKGCMC 2 cut(s) 597, 670
BamHI GGATCC 1 cut(s) 1363
BanI GGYRCC 2 cut(s) 509, 613
BanII GRGCYC 1 cut(s) 162
BauI CACGAG 2 cut(s) 663, 930
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 3 cut(s) 162, 597, 670
BbvCI CCTCAGC 1 cut(s) 165
BccI CCATC 2 cut(s) 500, 1375
BceAI ACGGC 3 cut(s) 91, 97, 254
BciT130I CCWGG 3 cut(s) 722, 1043, 1360
BclI TGATCA 1 cut(s) 15
BcoDI GTCTC 6 cut(s) 191, 544, 628, 666, 992, 1771
BfaI CTAG 7 cut(s) 426, 491, 539, 728, 854, 1118, 1584
BfmI CTRYAG 1 cut(s) 671
BfrI CTTAAG 1 cut(s) 263
BlpI GCTNAGC 1 cut(s) 156
BmcAI AGTACT 1 cut(s) 396
Bme1390I CCNGG 3 cut(s) 722, 1043, 1360
Bme18I GGWCC 3 cut(s) 418, 724, 1294
BmeT110I CYCGRG 1 cut(s) 1100
BmgT120I GGNCC 4 cut(s) 418, 724, 1013, 1294
BmiI GGNNCC 6 cut(s) 94, 511, 615, 1296, 1365, 1626
BmrFI CCNGG 3 cut(s) 722, 1043, 1360
BmrI ACTGGG 1 cut(s) 1003
BmsI GCATC 7 cut(s) 265, 458, 517, 650, 1114, 1481, 1657
BmuI ACTGGG 1 cut(s) 1003
BpiI GAAGAC 1 cut(s) 527
BpmI CTGGAG 1 cut(s) 1538
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 156
BpuEI CTTGAG 2 cut(s) 351, 364
BsaBI GATNNNNATC 2 cut(s) 983, 1512
BsaI GGTCTC 1 cut(s) 628
BsaJI CCNNGG 7 cut(s) 32, 81, 170, 1042, 1359, 1371, 1453
BsaWI WCCGGW 1 cut(s) 1108
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 994
Bse1I ACTGG 4 cut(s) 658, 817, 998, 1748
Bse3DI GCAATG 2 cut(s) 1439, 1616
Bse8I GATNNNNATC 2 cut(s) 983, 1512
BseAI TCCGGA 1 cut(s) 1108
BseBI CCWGG 3 cut(s) 722, 1043, 1360
BseDI CCNNGG 7 cut(s) 32, 81, 170, 1042, 1359, 1371, 1453
BseGI GGATG 5 cut(s) 116, 1105, 1367, 1496, 1642
BseJI GATNNNNATC 2 cut(s) 983, 1512
BseLI CCNNNNNNNGG 2 cut(s) 33, 994
BseMI GCAATG 2 cut(s) 1439, 1616
BseMII CTCAG 6 cut(s) 147, 179, 242, 539, 912, 1271
BseNI ACTGG 4 cut(s) 658, 817, 998, 1748
BseRI GAGGAG 4 cut(s) 88, 91, 112, 1865
BseSI GKGCMC 2 cut(s) 597, 670
BseYI CCCAGC 1 cut(s) 440
BshFI GGCC 2 cut(s) 269, 1015
BshNI GGYRCC 2 cut(s) 509, 613
BsiHKAI GWGCWC 3 cut(s) 162, 597, 670
BsiHKCI CYCGRG 1 cut(s) 1100
BsiSI CCGG 2 cut(s) 1109, 1695
BslFI GGGAC 1 cut(s) 1059
BslI CCNNNNNNNGG 2 cut(s) 33, 994
BsmAI GTCTC 6 cut(s) 191, 544, 628, 666, 992, 1771
BsmFI GGGAC 1 cut(s) 1059
BsnI GGCC 2 cut(s) 269, 1015
Bso31I GGTCTC 1 cut(s) 628
BsoBI CYCGRG 1 cut(s) 1100
Bsp1286I GDGCHC 3 cut(s) 162, 597, 670
Bsp13I TCCGGA 1 cut(s) 1108
Bsp1407I TGTACA 1 cut(s) 1561
Bsp1720I GCTNAGC 1 cut(s) 156
BspACI CCGC 6 cut(s) 8, 24, 44, 47, 337, 1128
BspANI GGCC 2 cut(s) 269, 1015
BspCNI CTCAG 6 cut(s) 148, 178, 241, 540, 911, 1272
BspEI TCCGGA 1 cut(s) 1108
BspHI TCATGA 2 cut(s) 1539, 1726
BspLI GGNNCC 6 cut(s) 94, 511, 615, 1296, 1365, 1626
BspPI GGATC 7 cut(s) 285, 640, 1130, 1358, 1371, 1621, 1705
BspT107I GGYRCC 2 cut(s) 509, 613
BspTI CTTAAG 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 628
BsrBI CCGCTC 1 cut(s) 337
BsrDI GCAATG 2 cut(s) 1439, 1616
BsrGI TGTACA 1 cut(s) 1561
BsrI ACTGG 4 cut(s) 658, 817, 998, 1748
BssECI CCNNGG 7 cut(s) 32, 81, 170, 1042, 1359, 1371, 1453
BssNAI GTATAC 1 cut(s) 1601
BssSI CACGAG 2 cut(s) 663, 930
BssT1I CCWWGG 4 cut(s) 32, 81, 1371, 1453
Bst1107I GTATAC 1 cut(s) 1601
Bst2BI CACGAG 2 cut(s) 663, 930
Bst2UI CCWGG 3 cut(s) 722, 1043, 1360
Bst4CI ACNGT 4 cut(s) 296, 1095, 1154, 1566
Bst6I CTCTTC 1 cut(s) 221
BstAFI CTTAAG 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 1561
BstC8I GCNNGC 4 cut(s) 348, 1166, 1470, 1549
BstDEI CTNAG 8 cut(s) 156, 165, 228, 548, 898, 1179, 1280, 1774
BstF5I GGATG 5 cut(s) 116, 1105, 1367, 1496, 1642
BstMAI GTCTC 6 cut(s) 191, 544, 628, 666, 992, 1771
BstMWI GCNNNNNNNGC 8 cut(s) 43, 46, 55, 166, 334, 343, 446, 1863
BstNI CCWGG 3 cut(s) 722, 1043, 1360
BstNSI RCATGY 2 cut(s) 1077, 1472
BstSCI CCNGG 3 cut(s) 720, 1041, 1358
BstSFI CTRYAG 1 cut(s) 671
BstSLI GKGCMC 2 cut(s) 597, 670
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 4 cut(s) 277, 632, 1363, 1697
BstXI CCANNNNNNTGG 1 cut(s) 1023
BstYI RGATCY 4 cut(s) 277, 632, 1363, 1697
BstZ17I GTATAC 1 cut(s) 1601
BsuRI GGCC 2 cut(s) 269, 1015
BtgZI GCGATG 1 cut(s) 1572
BtsCI GGATG 5 cut(s) 116, 1105, 1367, 1496, 1642
BtsIMutI CAGTG 3 cut(s) 301, 1100, 1628
Cac8I GCNNGC 4 cut(s) 348, 1166, 1470, 1549
CaiI CAGNNNCTG 1 cut(s) 310
CciI TCATGA 2 cut(s) 1539, 1726
Cfr13I GGNCC 4 cut(s) 418, 724, 1013, 1294
Csp6I GTAC 6 cut(s) 395, 603, 1056, 1562, 1581, 1847
CviQI GTAC 6 cut(s) 395, 603, 1056, 1562, 1581, 1847
DdeI CTNAG 8 cut(s) 156, 165, 228, 548, 898, 1179, 1280, 1774
DraI TTTAAA 1 cut(s) 1843
DraIII CACNNNGTG 1 cut(s) 592
DrdI GACNNNNNNGTC 2 cut(s) 416, 658
DseDI GACNNNNNNGTC 2 cut(s) 416, 658
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 4 cut(s) 32, 81, 1371, 1453
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 628
Eco47I GGWCC 3 cut(s) 418, 724, 1294
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 4 cut(s) 202, 516, 1524, 1652
Eco88I CYCGRG 1 cut(s) 1100
EcoICRI GAGCTC 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 1087
EcoRII CCWGG 3 cut(s) 720, 1041, 1358
EcoT14I CCWWGG 4 cut(s) 32, 81, 1371, 1453
EcoT22I ATGCAT 1 cut(s) 1496
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 4 cut(s) 32, 81, 1371, 1453
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 1059
FauI CCCGC 1 cut(s) 330
FauNDI CATATG 2 cut(s) 599, 646
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 1 cut(s) 1600
FokI GGATG 5 cut(s) 103, 1092, 1354, 1503, 1649
FriOI GRGCYC 1 cut(s) 162
FspBI CTAG 7 cut(s) 426, 491, 539, 728, 854, 1118, 1584
GsaI CCCAGC 1 cut(s) 444
GsuI CTGGAG 1 cut(s) 1538
HaeIII GGCC 2 cut(s) 269, 1015
HapII CCGG 2 cut(s) 1109, 1695
HincII GTYRAC 2 cut(s) 88, 788
HindII GTYRAC 2 cut(s) 88, 788
HindIII AAGCTT 2 cut(s) 1545, 1607
HinfI GANTC 7 cut(s) 175, 213, 410, 487, 764, 1062, 1282
HpaI GTTAAC 1 cut(s) 88
HpaII CCGG 2 cut(s) 1109, 1695
HphI GGTGA 2 cut(s) 709, 1142
Hpy166II GTNNAC 7 cut(s) 88, 595, 668, 788, 918, 1219, 1601
Hpy188III TCNNGA 8 cut(s) 368, 854, 878, 1109, 1157, 1517, 1540, 1727
Hpy8I GTNNAC 7 cut(s) 88, 595, 668, 788, 918, 1219, 1601
Hpy99I CGWCG 1 cut(s) 112
HpyCH4III ACNGT 4 cut(s) 296, 1095, 1154, 1566
HpyCH4IV ACGT 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 8 cut(s) 43, 46, 55, 166, 334, 343, 446, 1863
HpyF3I CTNAG 8 cut(s) 156, 165, 228, 548, 898, 1179, 1280, 1774
HpySE526I ACGT 1 cut(s) 204
Kpn2I TCCGGA 1 cut(s) 1108
Ksp22I TGATCA 1 cut(s) 15
KspAI GTTAAC 1 cut(s) 88
LmnI GCTCC 7 cut(s) 83, 92, 165, 334, 1465, 1851, 1862
LweI GCATC 7 cut(s) 265, 458, 517, 650, 1114, 1481, 1657
MaeI CTAG 7 cut(s) 426, 491, 539, 728, 854, 1118, 1584
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 4 cut(s) 469, 649, 835, 1182
MbiI CCGCTC 1 cut(s) 337
MboII GAAGA 7 cut(s) 208, 244, 532, 1134, 1376, 1418, 1517
MfeI CAATTG 1 cut(s) 1861
MflI RGATCY 4 cut(s) 277, 632, 1363, 1697
MhlI GDGCHC 3 cut(s) 162, 597, 670
MlyI GAGTC 5 cut(s) 207, 404, 496, 773, 1291
MmeI TCCRAC 4 cut(s) 312, 337, 610, 1272
Mph1103I ATGCAT 1 cut(s) 1496
MroI TCCGGA 1 cut(s) 1108
MseI TTAA 8 cut(s) 87, 264, 842, 1194, 1304, 1833, 1842, 1869
MslI CAYNNNNRTG 2 cut(s) 428, 1574
MspA1I CMGCKG 1 cut(s) 307
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 2 cut(s) 1109, 1695
MspR9I CCNGG 3 cut(s) 722, 1043, 1360
MunI CAATTG 1 cut(s) 1861
MvaI CCWGG 3 cut(s) 722, 1043, 1360
MwoI GCNNNNNNNGC 8 cut(s) 43, 46, 55, 166, 334, 343, 446, 1863
NdeI CATATG 2 cut(s) 599, 646
NlaIV GGNNCC 6 cut(s) 94, 511, 615, 1296, 1365, 1626
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 3 cut(s) 469, 649, 1182
NsiI ATGCAT 1 cut(s) 1496
NspI RCATGY 2 cut(s) 1077, 1472
PaeI GCATGC 1 cut(s) 1472
PaeR7I CTCGAG 1 cut(s) 1100
PagI TCATGA 2 cut(s) 1539, 1726
PcsI WCGNNNNNNNCGW 1 cut(s) 110
PfeI GAWTC 2 cut(s) 175, 1062
PflMI CCANNNNNTGG 1 cut(s) 33
PleI GAGTC 5 cut(s) 207, 404, 495, 772, 1290
PpsI GAGTC 5 cut(s) 207, 404, 495, 772, 1290
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 3 cut(s) 720, 1041, 1358
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 3 cut(s) 720, 1041, 1358
PspN4I GGNNCC 6 cut(s) 94, 511, 615, 1296, 1365, 1626
PspPI GGNCC 4 cut(s) 418, 724, 1013, 1294
PspXI VCTCGAGB 1 cut(s) 1100
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 4 cut(s) 277, 632, 1363, 1697
PvuII CAGCTG 1 cut(s) 307
RsaI GTAC 6 cut(s) 396, 604, 1057, 1563, 1582, 1848
RsaNI GTAC 6 cut(s) 395, 603, 1056, 1562, 1581, 1847
RseI CAYNNNNRTG 2 cut(s) 428, 1574
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 8 cut(s) 87, 264, 842, 1194, 1304, 1833, 1842, 1869
Sau96I GGNCC 4 cut(s) 418, 724, 1013, 1294
ScaI AGTACT 1 cut(s) 396
SchI GAGTC 5 cut(s) 207, 404, 496, 773, 1291
ScrFI CCNGG 3 cut(s) 722, 1043, 1360
SduI GDGCHC 3 cut(s) 162, 597, 670
SfaNI GCATC 7 cut(s) 265, 458, 517, 650, 1114, 1481, 1657
SfcI CTRYAG 1 cut(s) 671
Sfr274I CTCGAG 1 cut(s) 1100
SinI GGWCC 3 cut(s) 418, 724, 1294
SlaI CTCGAG 1 cut(s) 1100
SmiMI CAYNNNNRTG 2 cut(s) 428, 1574
SmlI CTYRAG 4 cut(s) 263, 366, 379, 1100
SmoI CTYRAG 4 cut(s) 263, 366, 379, 1100
SphI GCATGC 1 cut(s) 1472
SsiI CCGC 6 cut(s) 8, 24, 44, 47, 337, 1128
SspI AATATT 2 cut(s) 238, 1198
SspMI CTAG 7 cut(s) 426, 491, 539, 728, 854, 1118, 1584
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 720, 1041, 1358
StyI CCWWGG 4 cut(s) 32, 81, 1371, 1453
TaaI ACNGT 4 cut(s) 296, 1095, 1154, 1566
TaiI ACGT 1 cut(s) 207
TaqI TCGA 2 cut(s) 1101, 1404
TatI WGTACW 2 cut(s) 394, 1561
TauI GCSGC 2 cut(s) 11, 49
TfiI GAWTC 2 cut(s) 175, 1062
Tru1I TTAA 8 cut(s) 87, 264, 842, 1194, 1304, 1833, 1842, 1869
Tru9I TTAA 8 cut(s) 87, 264, 842, 1194, 1304, 1833, 1842, 1869
TscAI CASTG 3 cut(s) 301, 1100, 1635
TseFI GTSAC 3 cut(s) 469, 649, 1182
Tsp45I GTSAC 3 cut(s) 469, 649, 1182
TspDTI ATGAA 9 cut(s) 17, 747, 762, 782, 927, 1556, 1558, 1653, 1715
TspGWI ACGGA 3 cut(s) 105, 620, 1864
TspRI CASTG 3 cut(s) 301, 1100, 1635
Van91I CCANNNNNTGG 1 cut(s) 33
Vha464I CTTAAG 1 cut(s) 263
VneI GTGCAC 2 cut(s) 593, 666
VpaK11BI GGWCC 3 cut(s) 418, 724, 1294
XapI RAATTY 5 cut(s) 735, 804, 1087, 1393, 1649
XbaI TCTAGA 1 cut(s) 853
XceI RCATGY 2 cut(s) 1077, 1472
XhoI CTCGAG 1 cut(s) 1100
XmiI GTMKAC 1 cut(s) 1600
XspI CTAG 7 cut(s) 426, 491, 539, 728, 854, 1118, 1584
ZrmI AGTACT 1 cut(s) 396
Zsp2I ATGCAT 1 cut(s) 1496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.