Rorug02G0161800

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0018725
Physical Location & Seq
Reverse (-)
14473639 .. 14474782
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0161800.1

Sequence Viewer

Length: 681 bp
ATGGTTTCTCCTGGCCAGCAAACATTTTGTGCTACCACTCCCCATTTTTTCAAGGTTATTCTGGAGGACAATTCTAGAGACCTCAAACTGAAAATTCCAAAGAAATTTCTGATAAAATATGGTGAGGATCTCTTAAATACGGTATGTCTTAAACTTCCCAGTGGCTCTGAATGGGAAGTGGAATTGATAAGATGCAAGGGTAAGGCTTGGTTTGAAAAGGGTTGGCCAGAGTTCTCTAGGTTCTGCTGTCTTGACTACGGTAACTTCCTAGTTTTTCGATATGAAGGGAATTCTACATTCGAAGTATGTATATTTGATAGGACTGCCACGGGGATTGATTATCCTATAACTGTGCCCAAGATTTATGAAGATGAAAATGATGATTTATCTATTGAAATCTTGGAGTTTCCACCCTGCCCAAAAACAAGGGAGAAACCTTCATTACTATATCCTGATTATCCTCTTTGCAAGAAAATGAGACCAAGCTCGAGTACTAGATCAGACAGGATTTCTGAAAATGAGAGTGCTCAACCTGATTCCATGGTCTTGTTTGAGAAGTCATTGCCAAACAAAGATTCACATTGCTCTAATTCAGAGATGAAAGGTATAGAAATACTTTGTTTAATGCCAGAACTATTTAACAAGATGCAATGCCATTGTTCTTCTAATATCCTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

226

Amino Acids

26.06

Weight (kDa)

5.39

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 16 - 107 2.7e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66595
rosa_chinensis RchiOBHm_Chr2g0109671
rosa_multiflora Rmu_sc0000332.1_g000062 Rmu_sc0027285.1_g000001
rosa_rugosa Rorug02G0161800
rosa_samantha Rh2AG214100 Rh2BG224400 Rh2CG216400 Rh2DG219700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 135
AcoI YGGCCR 2 cut(s) 13, 224
AcsI RAATTY 3 cut(s) 93, 104, 289
AfaI GTAC 1 cut(s) 493
AgsI TTSAA 3 cut(s) 52, 215, 395
AjnI CCWGG 1 cut(s) 10
AjuI GAANNNNNNNTTGG 2 cut(s) 559, 591
AluBI AGCT 1 cut(s) 486
AluI AGCT 1 cut(s) 486
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 2 cut(s) 72, 472
AlwI GGATC 1 cut(s) 135
Ama87I CYCGRG 1 cut(s) 487
AoxI GGCC 2 cut(s) 13, 224
ApoI RAATTY 3 cut(s) 93, 104, 289
AsuHPI GGTGA 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 300
AvaI CYCGRG 1 cut(s) 487
BaeGI GKGCMC 1 cut(s) 357
BalI TGGCCA 2 cut(s) 15, 226
Bbv12I GWGCWC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 72, 472
BfaI CTAG 4 cut(s) 75, 237, 269, 495
BmcAI AGTACT 1 cut(s) 493
Bme1390I CCNGG 1 cut(s) 12
BmeT110I CYCGRG 1 cut(s) 487
BmrFI CCNGG 1 cut(s) 12
BmrI ACTGGG 1 cut(s) 153
BmsI GCATC 2 cut(s) 182, 636
BmuI ACTGGG 1 cut(s) 153
BpmI CTGGAG 1 cut(s) 83
Bpu14I TTCGAA 1 cut(s) 300
BsaI GGTCTC 2 cut(s) 72, 472
BsaJI CCNNGG 2 cut(s) 327, 540
Bse1I ACTGG 1 cut(s) 159
Bse3DI GCAATG 3 cut(s) 560, 580, 656
BseBI CCWGG 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 327, 540
BseMI GCAATG 3 cut(s) 560, 580, 656
BseNI ACTGG 1 cut(s) 159
BseSI GKGCMC 1 cut(s) 357
BshFI GGCC 2 cut(s) 15, 226
BsiHKAI GWGCWC 1 cut(s) 529
BsiHKCI CYCGRG 1 cut(s) 487
BsmAI GTCTC 2 cut(s) 72, 472
BsnI GGCC 2 cut(s) 15, 226
Bso31I GGTCTC 2 cut(s) 72, 472
BsoBI CYCGRG 1 cut(s) 487
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 2 cut(s) 357, 529
Bsp143I GATC 2 cut(s) 127, 497
Bsp19I CCATGG 1 cut(s) 540
BspANI GGCC 2 cut(s) 15, 226
BspPI GGATC 1 cut(s) 135
BspT104I TTCGAA 1 cut(s) 300
BspTNI GGTCTC 2 cut(s) 72, 472
BsrDI GCAATG 3 cut(s) 560, 580, 656
BsrI ACTGG 1 cut(s) 159
BssECI CCNNGG 2 cut(s) 327, 540
BssMI GATC 2 cut(s) 127, 497
BssT1I CCWWGG 1 cut(s) 540
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 142, 260, 352
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 17
BstDSI CCRYGG 2 cut(s) 327, 540
BstKTI GATC 2 cut(s) 130, 500
BstMAI GTCTC 2 cut(s) 72, 472
BstMBI GATC 2 cut(s) 127, 497
BstNI CCWGG 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 10
BstSLI GKGCMC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BsuRI GGCC 2 cut(s) 15, 226
BtgI CCRYGG 2 cut(s) 327, 540
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 492
CspCI CAANNNNNGTGG 2 cut(s) 316, 351
CviAII CATG 1 cut(s) 541
CviJI RGCY 5 cut(s) 15, 165, 206, 226, 486
CviKI_1 RGCY 5 cut(s) 15, 165, 206, 226, 486
CviQI GTAC 1 cut(s) 492
DpnI GATC 2 cut(s) 129, 499
DpnII GATC 2 cut(s) 127, 497
EaeI YGGCCR 2 cut(s) 13, 224
Eco130I CCWWGG 1 cut(s) 540
Eco31I GGTCTC 2 cut(s) 72, 472
Eco88I CYCGRG 1 cut(s) 487
EcoRI GAATTC 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 1 cut(s) 544
FatI CATG 1 cut(s) 540
FspBI CTAG 4 cut(s) 75, 237, 269, 495
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 2 cut(s) 15, 226
Hin1II CATG 1 cut(s) 544
HinfI GANTC 2 cut(s) 536, 575
HphI GGTGA 1 cut(s) 134
Hpy188I TCNGA 5 cut(s) 111, 169, 502, 514, 595
Hpy188III TCNNGA 4 cut(s) 62, 75, 251, 452
HpyAV CCTTC 2 cut(s) 278, 447
HpyCH4III ACNGT 3 cut(s) 142, 260, 352
HpyCH4V TGCA 3 cut(s) 195, 468, 649
Hsp92II CATG 1 cut(s) 544
Kzo9I GATC 2 cut(s) 127, 497
LweI GCATC 2 cut(s) 182, 636
MaeI CTAG 4 cut(s) 75, 237, 269, 495
MaeIII GTNAC 1 cut(s) 260
MalI GATC 2 cut(s) 129, 499
MboI GATC 2 cut(s) 127, 497
MboII GAAGA 2 cut(s) 380, 654
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 2 cut(s) 357, 529
MlsI TGGCCA 2 cut(s) 15, 226
MluCI AATT 6 cut(s) 70, 93, 104, 182, 289, 589
MluNI TGGCCA 2 cut(s) 15, 226
MnlI CCTC 4 cut(s) 58, 92, 118, 471
Mox20I TGGCCA 2 cut(s) 15, 226
MscI TGGCCA 2 cut(s) 15, 226
MseI TTAA 4 cut(s) 134, 150, 623, 639
Msp20I TGGCCA 2 cut(s) 15, 226
MspR9I CCNGG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 12
NcoI CCATGG 1 cut(s) 540
NdeII GATC 2 cut(s) 127, 497
NlaIII CATG 1 cut(s) 544
NspV TTCGAA 1 cut(s) 300
PaeR7I CTCGAG 1 cut(s) 487
PfeI GAWTC 2 cut(s) 536, 575
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspXI VCTCGAGB 1 cut(s) 487
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
SaqAI TTAA 4 cut(s) 134, 150, 623, 639
Sau3AI GATC 2 cut(s) 127, 497
ScaI AGTACT 1 cut(s) 493
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 2 cut(s) 357, 529
SetI ASST 7 cut(s) 57, 84, 242, 439, 488, 535, 607
SfaNI GCATC 2 cut(s) 182, 636
Sfr274I CTCGAG 1 cut(s) 487
SfuI TTCGAA 1 cut(s) 300
SlaI CTCGAG 1 cut(s) 487
SmlI CTYRAG 1 cut(s) 487
SmoI CTYRAG 1 cut(s) 487
Sse9I AATT 6 cut(s) 70, 93, 104, 182, 289, 589
SspMI CTAG 4 cut(s) 75, 237, 269, 495
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 3 cut(s) 142, 260, 352
TaqI TCGA 3 cut(s) 277, 300, 488
TasI AATT 6 cut(s) 70, 93, 104, 182, 289, 589
TatI WGTACW 1 cut(s) 491
TfiI GAWTC 2 cut(s) 536, 575
Tru1I TTAA 4 cut(s) 134, 150, 623, 639
Tru9I TTAA 4 cut(s) 134, 150, 623, 639
TscAI CASTG 1 cut(s) 166
TspDTI ATGAA 5 cut(s) 297, 381, 387, 429, 614
TspRI CASTG 1 cut(s) 166
XapI RAATTY 3 cut(s) 93, 104, 289
XbaI TCTAGA 1 cut(s) 74
XhoI CTCGAG 1 cut(s) 487
XspI CTAG 4 cut(s) 75, 237, 269, 495
ZrmI AGTACT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.