RchiOBHm_Chr2g0110721

pentatricopeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016599
Physical Location & Seq
Reverse (-)
22285049 .. 22288120
3072 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48435

Sequence Viewer

Length: 2160 bp
ATGGCCTTTGGTTTCCCCAGGCATTTCAATTTGTTTGCAACCTTGGAAATTGTAACACCAACTCTAAACCAACTCTCCCACGTCAAGTCCTTGACATCTCTTGCCCATAACACAAGCCACACAGAGGCCTCAAACTTTTACTCTTCTCTGATTCAACAATGCACACGGACAGAGTCCCTAAGAGGCTTGGGAATAGTTCAAACCCATATGATCAAATCCGGGTACATGCAACTCAGTATAGCCAATAGGCTCGTTGATGGGTGTTTGAAATGTGGTAGCTTAAGTAATGCACGTAAGGTGTTCGACGAATTGCCTGAGAGACATGTAGTCATCTGGAATGCGATGATTTCTTCATACATTGGCCACAAGAAGAGTAAGGAGGCTATTGGGTTGTATGGGAAGATGGTCTTTGATGGAGTTTTGCCGGATGAGTACACGTTTTCGAGTGTTTTCAAGGCTTTTTCGGGTCTGGGTCTTGTATATGAAGCGCGGAGGGCTCATGGGTTGTCAGTGGTTTTGGGTCTGGAGGTGGCAAATGTGTTTGTTGGTAGTGCTCTTGTTGATATGTATGCAAAGTTTGGTAGAATGGGGGATGCAAGGTTGGTGTCGAATCGCGTTGGGGAGAAAGATGTTGTTTTGTTTACAGCGCTGATTGTTGGTTACTCTCAGCATGGTGATGATGGTGAAGCTCTTGAGGTTTTTGGGGACATGATCAACCAAGGAGTTAAGGCTAATGAGTATACTTTTGCTAGCATCTTGATCACTTGTGGGAACTTGGAGGATTTAACTCATGGTAAGTTGGTCCATGGTCTTGTTGTCAAATCTGGTTGTGAAGCTGCTGTGGCGTCACAGACTTCACTTCTAACAATGTATGCTAGGTGTGGATTGATTGATGATTCTCTGAGGATTTTTAAAAGGTTTCATCATCCAAACCTGGTAACTTGGACGTCTCTTATAGTGGCTCTTGTTCGGAATGGTAGAGAAGAGTTGGCTTTGATAAAGTTTAGAAAGATGATTCGGGATTCAGTAGTTCCAAATTCTTTTACTTTATCTAGTGTTCTTCAGGCATGCTCAAGCCTTGCTATGATGGATGAAGGACAGCAAATTCATGCCATGGTCACAAAGTTCGGATTAGATAGACACATATTTGCTGGTGCTGCTCTCATTAACTTGTATGGGAAATGCGGAAATACAGAGATGGCAAGGTCAGTTTTTGATGCCTTGATTGAAATTGATTTGGTCTCCGTGAATTCCATGATATATAGCTATGCGCAGAATGGATTTGGACATGAAGCACTTCAGCTTTTTGATGGGATGAAAGACTTGAGACTTGAACCAAACGATGTTACAATTTTGAGTGTTCTCTTGGCATGCAATAATTCAGGATTGGTGCAACAAGGCTGCCAAATTTTTTCTACCATTAGGAGTAATCACAATATTGAGTTGACAAGGGATCACTATGCTTGTATGGTTGATTTGCTGGGGCGTGCTGGAAGACTTGAAGATGCCGAAATGTTGATAAAGGAAGTCAAAAATCCAGATTTAGTATTATGGAGGGCACTGCTAAGTGCATGTAAACTTCATGGACAGGTTGAGATGGCAGAAAGAGCTCTAAAGAAGGTGCTTGAACTTGCTCCAGGAGACGGAGGATCTCATGTCCTGTTGACAAATCTTTATGCTTCAAATGGTAATTGGAGTCAAGTTATTGATATGAAAAGTACAATGAGGGAGATGAAATTCCAGAAGAACCCGGCAATGAGTTGGGTTGATGTGGACAGGGAGGTTCACACTTTCATGGCTGGAGATTTGTCCCATTCAAGATCTAGAGAGATAAATGAAACCTTAGAGAAATTGTTTGAGGAGGTGCAAATATTGGGTTATGTTCCTGATACAAGATTTGTGTTACAGGATTTGGATGAGGAGAAGAAGAAGAGGTCATTGTACTATCACAGCGAAAAGCTAGCCATTGCCTTTGCTCTATTAATGAGTAGTAAAAAGAATACCATCATCAGGATATTCAAGAACCTTAAAGTTTGTGGCGACTGTCATTCCTGGATAAAATTTGTCACCAGAGTTATTGGGAGAGATATAATTGCTCGGGACGCTAAAAGATTTCATCATTTTAAGGATGGGTGTTGTTCCTGTAGAGATTATTGGTGA

Protein Analysis

719

Amino Acids

80.33

Weight (kDa)

7.84

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 154 6.6e-12 PPR repeat family
PPR_2 PF13041 209 - 254 1.7e-08 PPR repeat family
PPR_3 PF13812 209 - 258 8.9e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 310 - 358 5.9e-08 PPR repeat family
PPR_2 PF13041 412 - 457 1.7e-06 PPR repeat family
PPR PF01535 414 - 442 6.1e-06 PPR repeat
E_motif PF20431 528 - 590 4.8e-14 E motif
DYW_deaminase PF14432 626 - 719 4.2e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016599)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65570
malus_domestica MD15G1309800.v1.1
prunus_persica Prupe.6G204100_v2.0.a1 Prupe.6G204100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0110721
rosa_laevigata RLG00000017836
rosa_multiflora Rmu_sc0000389.1_g000048
rosa_rugosa Rorug02G0168100 Rorug02G0169300
rosa_samantha Rh2AG221300 Rh2BG231500 Rh2CG223800 Rh2DG227500
rosa_wichuraiana Rw2G017090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 950
Acc16I TGCGCA 1 cut(s) 1272
AccI GTMKAC 1 cut(s) 740
AccII CGCG 2 cut(s) 490, 615
AciI CCGC 2 cut(s) 490, 1185
AclWI GGATC 2 cut(s) 1461, 1657
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 6 cut(s) 1036, 1104, 1249, 1407, 1736, 2060
AcuI CTGAAG 2 cut(s) 1046, 1283
AcyI GRCGYC 2 cut(s) 845, 947
AfaI GTAC 4 cut(s) 224, 434, 1720, 1943
AfeI AGCGCT 1 cut(s) 648
AfiI CCNNNNNNNGG 3 cut(s) 124, 1643, 2010
AflII CTTAAG 1 cut(s) 280
AflIII ACRYGT 2 cut(s) 322, 435
AhdI GACNNNNNGTC 1 cut(s) 326
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 4 cut(s) 17, 933, 1636, 2051
AluBI AGCT 7 cut(s) 279, 689, 836, 1266, 1303, 1610, 1960
AluI AGCT 7 cut(s) 279, 689, 836, 1266, 1303, 1610, 1960
Alw21I GWGCWC 2 cut(s) 556, 1612
Alw26I GTCTC 5 cut(s) 313, 954, 1246, 1321, 1635
AlwI GGATC 2 cut(s) 1461, 1657
Ama87I CYCGRG 1 cut(s) 2097
Aor51HI AGCGCT 1 cut(s) 648
AoxI GGCC 3 cut(s) 3, 126, 361
ApeKI GCWGC 3 cut(s) 836, 1157, 1401
ApoI RAATTY 6 cut(s) 1036, 1104, 1249, 1407, 1736, 2060
AseI ATTAAT 1 cut(s) 1982
Asp700I GAANNNNTTC 1 cut(s) 1296
AspLEI GCGC 3 cut(s) 490, 649, 1273
AspS9I GGNCC 1 cut(s) 802
AsuC2I CCSGG 2 cut(s) 220, 1751
AsuHPI GGTGA 3 cut(s) 686, 695, 2059
AsuNHI GCTAGC 2 cut(s) 749, 1960
AvaI CYCGRG 1 cut(s) 2097
AvaII GGWCC 1 cut(s) 802
BaeGI GKGCMC 1 cut(s) 1561
BalI TGGCCA 1 cut(s) 363
BanII GRGCYC 2 cut(s) 499, 1612
BbsI GAAGAC 1 cut(s) 1501
Bbv12I GWGCWC 2 cut(s) 556, 1612
BbvI GCAGC 3 cut(s) 823, 1144, 1388
BciT130I CCWGG 4 cut(s) 19, 935, 1638, 2053
BclI TGATCA 3 cut(s) 210, 711, 759
BcnI CCSGG 2 cut(s) 220, 1751
BcoDI GTCTC 5 cut(s) 313, 954, 1246, 1321, 1635
BfaI CTAG 5 cut(s) 750, 876, 1053, 1824, 1961
BfmI CTRYAG 1 cut(s) 2143
BfoI RGCGCY 1 cut(s) 650
BfrI CTTAAG 1 cut(s) 280
BglII AGATCT 1 cut(s) 1820
BisI GCNGC 3 cut(s) 837, 1158, 1402
BlsI GCNGC 3 cut(s) 838, 1159, 1403
Bme1390I CCNGG 6 cut(s) 19, 220, 935, 1638, 1751, 2053
Bme18I GGWCC 1 cut(s) 802
BmeRI GACNNNNNGTC 1 cut(s) 326
BmeT110I CYCGRG 1 cut(s) 2097
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 802
BmrFI CCNGG 6 cut(s) 19, 220, 935, 1638, 1751, 2053
BmsI GCATC 4 cut(s) 583, 762, 1207, 1495
BmtI GCTAGC 2 cut(s) 753, 1964
BpiI GAAGAC 1 cut(s) 1501
BpmI CTGGAG 3 cut(s) 545, 1620, 1821
BpuEI CTTGAG 3 cut(s) 713, 1057, 1345
BpuMI CCSGG 2 cut(s) 220, 1751
BsaAI YACGTR 1 cut(s) 293
BsaHI GRCGYC 2 cut(s) 845, 947
BsaI GGTCTC 1 cut(s) 1246
BsaJI CCNNGG 5 cut(s) 17, 42, 718, 805, 1113
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 1643, 2010
Bse3DI GCAATG 2 cut(s) 1761, 1965
BseBI CCWGG 4 cut(s) 19, 935, 1638, 2053
BseDI CCNNGG 5 cut(s) 17, 42, 718, 805, 1113
BseGI GGATG 7 cut(s) 433, 598, 925, 1096, 1320, 1921, 2134
BseLI CCNNNNNNNGG 3 cut(s) 124, 1643, 2010
BseMI GCAATG 2 cut(s) 1761, 1965
BseMII CTCAG 4 cut(s) 247, 306, 680, 893
BseRI GAGGAG 2 cut(s) 1874, 1934
BseSI GKGCMC 1 cut(s) 1561
BseXI GCAGC 3 cut(s) 823, 1144, 1388
BseYI CCCAGC 1 cut(s) 1480
Bsh1236I CGCG 2 cut(s) 490, 615
BshFI GGCC 3 cut(s) 5, 128, 363
BsiHKAI GWGCWC 2 cut(s) 556, 1612
BsiHKCI CYCGRG 1 cut(s) 2097
BsiSI CCGG 3 cut(s) 219, 425, 1751
BslFI GGGAC 4 cut(s) 160, 719, 1795, 2114
BslI CCNNNNNNNGG 3 cut(s) 124, 1643, 2010
BsmAI GTCTC 5 cut(s) 313, 954, 1246, 1321, 1635
BsmBI CGTCTC 2 cut(s) 954, 1635
BsmFI GGGAC 4 cut(s) 160, 719, 1795, 2114
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 3 cut(s) 5, 128, 363
Bso31I GGTCTC 1 cut(s) 1246
BsoBI CYCGRG 1 cut(s) 2097
Bsp1286I GDGCHC 4 cut(s) 499, 556, 1561, 1612
Bsp143I GATC 6 cut(s) 210, 711, 759, 1453, 1649, 1820
Bsp19I CCATGG 2 cut(s) 805, 1113
BspACI CCGC 2 cut(s) 490, 1185
BspANI GGCC 3 cut(s) 5, 128, 363
BspCNI CTCAG 4 cut(s) 246, 307, 679, 894
BspFNI CGCG 2 cut(s) 490, 615
BspOI GCTAGC 2 cut(s) 753, 1964
BspPI GGATC 2 cut(s) 1461, 1657
BspTI CTTAAG 1 cut(s) 280
BspTNI GGTCTC 1 cut(s) 1246
BsrDI GCAATG 2 cut(s) 1761, 1965
BssECI CCNNGG 5 cut(s) 17, 42, 718, 805, 1113
BssMI GATC 6 cut(s) 210, 711, 759, 1453, 1649, 1820
BssNAI GTATAC 1 cut(s) 741
BssNI GRCGYC 2 cut(s) 845, 947
BssT1I CCWWGG 4 cut(s) 42, 718, 805, 1113
Bst1107I GTATAC 1 cut(s) 741
Bst2UI CCWGG 4 cut(s) 19, 935, 1638, 2053
Bst4CI ACNGT 1 cut(s) 2045
Bst6I CTCTTC 4 cut(s) 148, 365, 978, 1925
BstACI GRCGYC 2 cut(s) 845, 947
BstAFI CTTAAG 1 cut(s) 280
BstBAI YACGTR 1 cut(s) 293
BstC8I GCNNGC 5 cut(s) 751, 1069, 1372, 1488, 1962
BstDEI CTNAG 7 cut(s) 179, 233, 315, 666, 902, 1565, 1843
BstDSI CCRYGG 2 cut(s) 805, 1113
BstF5I GGATG 7 cut(s) 433, 598, 925, 1096, 1320, 1921, 2134
BstFNI CGCG 2 cut(s) 490, 615
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 3 cut(s) 490, 649, 1273
BstKTI GATC 6 cut(s) 213, 714, 762, 1456, 1652, 1823
BstMAI GTCTC 5 cut(s) 313, 954, 1246, 1321, 1635
BstMBI GATC 6 cut(s) 210, 711, 759, 1453, 1649, 1820
BstMWI GCNNNNNNNGC 5 cut(s) 494, 842, 1157, 1607, 2102
BstNI CCWGG 4 cut(s) 19, 935, 1638, 2053
BstNSI RCATGY 5 cut(s) 229, 326, 1071, 1374, 1575
BstSCI CCNGG 6 cut(s) 17, 218, 933, 1636, 1749, 2051
BstSFI CTRYAG 1 cut(s) 2143
BstSLI GKGCMC 1 cut(s) 1561
BstUI CGCG 2 cut(s) 490, 615
BstV1I GCAGC 3 cut(s) 823, 1144, 1388
BstV2I GAAGAC 1 cut(s) 1501
BstX2I RGATCY 2 cut(s) 1649, 1820
BstYI RGATCY 2 cut(s) 1649, 1820
BstZ17I GTATAC 1 cut(s) 741
BsuRI GGCC 3 cut(s) 5, 128, 363
BtgI CCRYGG 2 cut(s) 805, 1113
BtgZI GCGATG 1 cut(s) 356
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 7 cut(s) 433, 598, 925, 1096, 1320, 1921, 2134
BtsI GCAGTG 1 cut(s) 1559
BtsIMutI CAGTG 2 cut(s) 516, 1559
Cac8I GCNNGC 5 cut(s) 751, 1069, 1372, 1488, 1962
CfoI GCGC 3 cut(s) 490, 649, 1273
Cfr13I GGNCC 1 cut(s) 802
CseI GACGC 2 cut(s) 834, 2111
CsiI ACCWGGT 1 cut(s) 933
Csp6I GTAC 4 cut(s) 223, 433, 1719, 1942
CviQI GTAC 4 cut(s) 223, 433, 1719, 1942
DdeI CTNAG 7 cut(s) 179, 233, 315, 666, 902, 1565, 1843
DpnI GATC 6 cut(s) 212, 713, 761, 1455, 1651, 1822
DpnII GATC 6 cut(s) 210, 711, 759, 1453, 1649, 1820
DraI TTTAAA 1 cut(s) 913
DriI GACNNNNNGTC 1 cut(s) 326
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 4 cut(s) 148, 365, 978, 1925
Eam1105I GACNNNNNGTC 1 cut(s) 326
EarI CTCTTC 4 cut(s) 148, 365, 978, 1925
Ecl136II GAGCTC 1 cut(s) 1610
Eco130I CCWWGG 4 cut(s) 42, 718, 805, 1113
Eco147I AGGCCT 1 cut(s) 128
Eco24I GRGCYC 2 cut(s) 499, 1612
Eco31I GGTCTC 1 cut(s) 1246
Eco47I GGWCC 1 cut(s) 802
Eco47III AGCGCT 1 cut(s) 648
Eco53kI GAGCTC 1 cut(s) 1610
Eco57I CTGAAG 2 cut(s) 1046, 1283
Eco88I CYCGRG 1 cut(s) 2097
EcoICRI GAGCTC 1 cut(s) 1610
EcoRI GAATTC 1 cut(s) 1249
EcoRII CCWGG 4 cut(s) 17, 933, 1636, 2051
EcoT14I CCWWGG 4 cut(s) 42, 718, 805, 1113
EcoT38I GRGCYC 2 cut(s) 499, 1612
ErhI CCWWGG 4 cut(s) 42, 718, 805, 1113
Esp3I CGTCTC 2 cut(s) 954, 1635
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 4 cut(s) 160, 719, 1795, 2114
FauNDI CATATG 1 cut(s) 207
FbaI TGATCA 3 cut(s) 210, 711, 759
FblI GTMKAC 1 cut(s) 740
Fnu4HI GCNGC 3 cut(s) 837, 1158, 1402
FokI GGATG 7 cut(s) 440, 605, 912, 1103, 1327, 1928, 2141
FriOI GRGCYC 2 cut(s) 499, 1612
Fsp4HI GCNGC 3 cut(s) 837, 1158, 1402
FspBI CTAG 5 cut(s) 750, 876, 1053, 1824, 1961
FspI TGCGCA 1 cut(s) 1272
GlaI GCGC 3 cut(s) 489, 648, 1272
GluI GCNGC 3 cut(s) 837, 1158, 1402
GsaI CCCAGC 1 cut(s) 1484
GsuI CTGGAG 3 cut(s) 545, 1620, 1821
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 3 cut(s) 5, 128, 363
HapII CCGG 3 cut(s) 219, 425, 1751
HgaI GACGC 2 cut(s) 834, 2111
HhaI GCGC 3 cut(s) 490, 649, 1273
Hin1I GRCGYC 2 cut(s) 845, 947
Hin6I GCGC 3 cut(s) 488, 647, 1271
HinP1I GCGC 3 cut(s) 488, 647, 1271
HincII GTYRAC 2 cut(s) 1446, 1665
HindII GTYRAC 2 cut(s) 1446, 1665
HinfI GANTC 7 cut(s) 151, 173, 610, 896, 1015, 1022, 1696
HpaII CCGG 3 cut(s) 219, 425, 1751
HphI GGTGA 3 cut(s) 686, 695, 2059
Hpy166II GTNNAC 8 cut(s) 435, 642, 741, 1446, 1577, 1665, 1774, 1786
Hpy188I TCNGA 4 cut(s) 150, 903, 972, 1130
Hpy8I GTNNAC 8 cut(s) 435, 642, 741, 1446, 1577, 1665, 1774, 1786
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 2 cut(s) 1088, 1612
HpyCH4III ACNGT 1 cut(s) 2045
HpyCH4IV ACGT 4 cut(s) 81, 292, 437, 947
HpyF10VI GCNNNNNNNGC 5 cut(s) 494, 842, 1157, 1607, 2102
HpyF3I CTNAG 7 cut(s) 179, 233, 315, 666, 902, 1565, 1843
HpySE526I ACGT 4 cut(s) 81, 292, 437, 947
Hsp92I GRCGYC 2 cut(s) 845, 947
HspAI GCGC 3 cut(s) 488, 647, 1271
Ksp22I TGATCA 3 cut(s) 210, 711, 759
Kzo9I GATC 6 cut(s) 210, 711, 759, 1453, 1649, 1820
LmnI GCTCC 1 cut(s) 1639
Lsp1109I GCAGC 3 cut(s) 823, 1144, 1388
LweI GCATC 4 cut(s) 583, 762, 1207, 1495
MabI ACCWGGT 1 cut(s) 933
MaeI CTAG 5 cut(s) 750, 876, 1053, 1824, 1961
MaeII ACGT 4 cut(s) 81, 292, 437, 947
MaeIII GTNAC 8 cut(s) 52, 659, 846, 937, 1117, 1345, 1902, 2065
MalI GATC 6 cut(s) 212, 713, 761, 1455, 1651, 1822
MboI GATC 6 cut(s) 210, 711, 759, 1453, 1649, 1820
MflI RGATCY 2 cut(s) 1649, 1820
MhlI GDGCHC 4 cut(s) 499, 556, 1561, 1612
MlsI TGGCCA 1 cut(s) 363
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 2 cut(s) 182, 1705
Mox20I TGGCCA 1 cut(s) 363
MroXI GAANNNNTTC 1 cut(s) 1296
MscI TGGCCA 1 cut(s) 363
MseI TTAA 8 cut(s) 281, 726, 785, 912, 1167, 1982, 2028, 2124
MslI CAYNNNNRTG 2 cut(s) 675, 1793
Msp20I TGGCCA 1 cut(s) 363
MspCI CTTAAG 1 cut(s) 280
MspI CCGG 3 cut(s) 219, 425, 1751
MspR9I CCNGG 6 cut(s) 19, 220, 935, 1638, 1751, 2053
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 4 cut(s) 19, 935, 1638, 2053
MvnI CGCG 2 cut(s) 490, 615
MwoI GCNNNNNNNGC 5 cut(s) 494, 842, 1157, 1607, 2102
NciI CCSGG 2 cut(s) 220, 1751
NcoI CCATGG 2 cut(s) 805, 1113
NdeI CATATG 1 cut(s) 207
NdeII GATC 6 cut(s) 210, 711, 759, 1453, 1649, 1820
NheI GCTAGC 2 cut(s) 749, 1960
NmuCI GTSAC 3 cut(s) 846, 1117, 2065
NsbI TGCGCA 1 cut(s) 1272
NspI RCATGY 5 cut(s) 229, 326, 1071, 1374, 1575
PaeI GCATGC 2 cut(s) 1071, 1374
PceI AGGCCT 1 cut(s) 128
PciI ACATGT 1 cut(s) 322
PctI GAATGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 1296
PfeI GAWTC 5 cut(s) 151, 610, 896, 1015, 1022
PflFI GACNNNGTC 1 cut(s) 172
PfoI TCCNGGA 2 cut(s) 1636, 2051
PkrI GCNGC 3 cut(s) 838, 1159, 1403
PleI GAGTC 2 cut(s) 181, 1704
PpsI GAGTC 2 cut(s) 181, 1704
Ppu21I YACGTR 1 cut(s) 293
PscI ACATGT 1 cut(s) 322
PshBI ATTAAT 1 cut(s) 1982
Psp124BI GAGCTC 1 cut(s) 1612
Psp6I CCWGG 4 cut(s) 17, 933, 1636, 2051
PspFI CCCAGC 1 cut(s) 1480
PspGI CCWGG 4 cut(s) 17, 933, 1636, 2051
PspPI GGNCC 1 cut(s) 802
PsuI RGATCY 2 cut(s) 1649, 1820
PsyI GACNNNGTC 1 cut(s) 172
RsaI GTAC 4 cut(s) 224, 434, 1720, 1943
RsaNI GTAC 4 cut(s) 223, 433, 1719, 1942
RseI CAYNNNNRTG 2 cut(s) 675, 1793
SacI GAGCTC 1 cut(s) 1612
SaqAI TTAA 8 cut(s) 281, 726, 785, 912, 1167, 1982, 2028, 2124
SatI GCNGC 3 cut(s) 837, 1158, 1402
Sau3AI GATC 6 cut(s) 210, 711, 759, 1453, 1649, 1820
Sau96I GGNCC 1 cut(s) 802
SchI GAGTC 2 cut(s) 182, 1705
ScrFI CCNGG 6 cut(s) 19, 220, 935, 1638, 1751, 2053
SduI GDGCHC 4 cut(s) 499, 556, 1561, 1612
SexAI ACCWGGT 1 cut(s) 933
SfaNI GCATC 4 cut(s) 583, 762, 1207, 1495
SfcI CTRYAG 1 cut(s) 2143
SinI GGWCC 1 cut(s) 802
SmiMI CAYNNNNRTG 2 cut(s) 675, 1793
SmlI CTYRAG 4 cut(s) 280, 692, 1072, 1324
SmoI CTYRAG 4 cut(s) 280, 692, 1072, 1324
SphI GCATGC 2 cut(s) 1071, 1374
SseBI AGGCCT 1 cut(s) 128
SsiI CCGC 2 cut(s) 490, 1185
SspI AATATT 2 cut(s) 1438, 1872
SspMI CTAG 5 cut(s) 750, 876, 1053, 1824, 1961
SstI GAGCTC 1 cut(s) 1612
StuI AGGCCT 1 cut(s) 128
StyD4I CCNGG 6 cut(s) 17, 218, 933, 1636, 1749, 2051
StyI CCWWGG 4 cut(s) 42, 718, 805, 1113
TaaI ACNGT 1 cut(s) 2045
TaiI ACGT 4 cut(s) 84, 295, 440, 950
TaqI TCGA 3 cut(s) 303, 443, 608
TatI WGTACW 3 cut(s) 432, 1718, 1941
TfiI GAWTC 5 cut(s) 151, 610, 896, 1015, 1022
Tru1I TTAA 8 cut(s) 281, 726, 785, 912, 1167, 1982, 2028, 2124
Tru9I TTAA 8 cut(s) 281, 726, 785, 912, 1167, 1982, 2028, 2124
TscAI CASTG 2 cut(s) 516, 1566
TseFI GTSAC 3 cut(s) 846, 1117, 2065
TseI GCWGC 3 cut(s) 836, 1157, 1401
Tsp45I GTSAC 3 cut(s) 846, 1117, 2065
TspGWI ACGGA 3 cut(s) 181, 1234, 1659
TspRI CASTG 2 cut(s) 516, 1566
Tth111I GACNNNGTC 1 cut(s) 172
Vha464I CTTAAG 1 cut(s) 280
VpaK11BI GGWCC 1 cut(s) 802
VspI ATTAAT 1 cut(s) 1982
XapI RAATTY 6 cut(s) 1036, 1104, 1249, 1407, 1736, 2060
XbaI TCTAGA 1 cut(s) 1823
XceI RCATGY 5 cut(s) 229, 326, 1071, 1374, 1575
XmiI GTMKAC 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 1296
XspI CTAG 5 cut(s) 750, 876, 1053, 1824, 1961
ZraI GACGTC 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.