RchiOBHm_Chr2g0135431

histidine-containing phosphotransfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014510
Physical Location & Seq
Forward (+)
52475944 .. 52477962
2019 bp
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UTR
Exon/CDS
Intron
PRQ50633

Sequence Viewer

Length: 477 bp
ATGTTGGGGTTGGGTGTTGATCGGTTGCGAGCCGATATGAATCGGTTGCTCGCAATGCTCTTTCATCAGGGAGTATTGGATGAGCAATTCTTGCAACTGCAGCAGCTTCAAGACGAGAGCTCCCCAAACTTTGTTTCTGAAGTTGTCAACATCTACTTCCAAGAGTCGGAGAAGCTCTTGAGAAATCTCAGAGCGCTTCTGATGGATAGGGAGTTCTCAGACTACAAGAAAATGGGAATCCATTTGAATCAGTTCATCGGAAGCAGCTCAAGCATTGGTGCCAAGAGACTCAGAAACGTCTGCGTTGCTTTTCGAGCCGCTTCTGAACAACATAACCGTGCTGGGTGCTTGAGAGCTTTAGAGCTGCTGGAACATGACTATTGCTACCTTAAGAATAAACTGCACGAGTTATTCCAGATAGAGCAGCAACGAGTGTTGGCAGCTGGGGTTCGGTACCCAATACAGCAGATCAATTAA

Protein Analysis

158

Amino Acids

18.32

Weight (kDa)

7.77

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hpt PF01627 47 - 126 1.2e-07 Hpt domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014510)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80100 AT1G80100 AT1G80100
fragaria_vesca FvH4_6g29740
malus_domestica MD17G1211900.v1.1
prunus_persica Prupe.3G087100_v2.0.a1 Prupe.3G087100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0135431
rosa_laevigata RLG00000019464
rosa_roxburghii Rroxscaffold_2G00109250 Rroxscaffold_2G00116740
rosa_rugosa Rorug02G0324700
rosa_samantha Rh2AG370100 Rh2BG382000 Rh2CG360700 Rh2DG398300
rosa_wichuraiana Rw2G030680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 453
AccB1I GGYRCC 2 cut(s) 278, 453
AciI CCGC 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 1 cut(s) 455
AfeI AGCGCT 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 166
AflII CTTAAG 1 cut(s) 389
AgsI TTSAA 2 cut(s) 110, 247
AjuI GAANNNNNNNTTGG 2 cut(s) 118, 150
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 7 cut(s) 106, 120, 175, 267, 356, 364, 443
AluI AGCT 7 cut(s) 106, 120, 175, 267, 356, 364, 443
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 1 cut(s) 280
Aor51HI AGCGCT 1 cut(s) 195
ApeKI GCWGC 6 cut(s) 100, 103, 264, 364, 424, 440
Asp700I GAANNNNTTC 1 cut(s) 251
Asp718I GGTACC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 196
BanI GGYRCC 2 cut(s) 278, 453
BanII GRGCYC 1 cut(s) 122
BauI CACGAG 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 6 cut(s) 112, 115, 276, 351, 436, 452
BccI CCATC 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 280
BfmI CTRYAG 1 cut(s) 98
BfoI RGCGCY 1 cut(s) 197
BfrI CTTAAG 1 cut(s) 389
BisI GCNGC 7 cut(s) 101, 104, 265, 318, 365, 425, 441
BlsI GCNGC 7 cut(s) 102, 105, 266, 319, 366, 426, 442
BmiI GGNNCC 2 cut(s) 280, 455
BpuEI CTTGAG 3 cut(s) 199, 253, 370
BsaBI GATNNNNATC 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 39
BseGI GGATG 1 cut(s) 85
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 3 cut(s) 202, 231, 304
BseXI GCAGC 6 cut(s) 112, 115, 276, 351, 436, 452
BseYI CCCAGC 2 cut(s) 341, 443
BsgI GTGCAG 1 cut(s) 386
BshNI GGYRCC 2 cut(s) 278, 453
BsiHKAI GWGCWC 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 122
Bsp143I GATC 2 cut(s) 19, 468
BspACI CCGC 1 cut(s) 318
BspCNI CTCAG 3 cut(s) 201, 230, 303
BspLI GGNNCC 2 cut(s) 280, 455
BspMAI CTGCAG 1 cut(s) 102
BspT107I GGYRCC 2 cut(s) 278, 453
BspTI CTTAAG 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 60
BssMI GATC 2 cut(s) 19, 468
BssSI CACGAG 1 cut(s) 404
Bst2BI CACGAG 1 cut(s) 404
Bst4CI ACNGT 1 cut(s) 338
BstAFI CTTAAG 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 91
BstC8I GCNNGC 2 cut(s) 30, 51
BstDEI CTNAG 3 cut(s) 188, 217, 290
BstF5I GGATG 1 cut(s) 85
BstH2I RGCGCY 1 cut(s) 197
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 2 cut(s) 22, 471
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 2 cut(s) 19, 468
BstMWI GCNNNNNNNGC 5 cut(s) 55, 91, 100, 270, 314
BstSFI CTRYAG 1 cut(s) 98
BstV1I GCAGC 6 cut(s) 112, 115, 276, 351, 436, 452
BtsCI GGATG 1 cut(s) 85
Cac8I GCNNGC 2 cut(s) 30, 51
CfoI GCGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 454
CviAII CATG 1 cut(s) 374
CviJI RGCY 9 cut(s) 32, 106, 120, 175, 267, 317, 356, 364, 443
CviKI_1 RGCY 9 cut(s) 32, 106, 120, 175, 267, 317, 356, 364, 443
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 3 cut(s) 188, 217, 290
DpnI GATC 2 cut(s) 21, 470
DpnII GATC 2 cut(s) 19, 468
Ecl136II GAGCTC 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 122
Eco47III AGCGCT 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 122
FaeI CATG 1 cut(s) 377
FaiI YATR 3 cut(s) 38, 333, 375
FatI CATG 1 cut(s) 373
Fnu4HI GCNGC 7 cut(s) 101, 104, 265, 318, 365, 425, 441
FokI GGATG 1 cut(s) 92
FriOI GRGCYC 1 cut(s) 122
Fsp4HI GCNGC 7 cut(s) 101, 104, 265, 318, 365, 425, 441
GlaI GCGC 1 cut(s) 195
GluI GCNGC 7 cut(s) 101, 104, 265, 318, 365, 425, 441
GsaI CCCAGC 2 cut(s) 345, 447
HaeII RGCGCY 1 cut(s) 197
HhaI GCGC 1 cut(s) 196
Hin1II CATG 1 cut(s) 377
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 5 cut(s) 40, 164, 237, 247, 288
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 8 cut(s) 139, 169, 191, 201, 220, 260, 293, 325
Hpy188III TCNNGA 3 cut(s) 110, 178, 415
Hpy8I GTNNAC 1 cut(s) 148
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 3 cut(s) 94, 100, 403
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 91, 100, 270, 314
HpyF3I CTNAG 3 cut(s) 188, 217, 290
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 1 cut(s) 377
HspAI GCGC 1 cut(s) 194
KpnI GGTACC 1 cut(s) 457
Kzo9I GATC 2 cut(s) 19, 468
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 5 cut(s) 53, 327, 353, 428, 429
Lsp1109I GCAGC 6 cut(s) 112, 115, 276, 351, 436, 452
MaeII ACGT 1 cut(s) 297
MalI GATC 2 cut(s) 21, 470
MboI GATC 2 cut(s) 19, 468
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 2 cut(s) 86, 472
MlyI GAGTC 2 cut(s) 173, 282
MmeI TCCRAC 1 cut(s) 147
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 2 cut(s) 390, 475
MslI CAYNNNNRTG 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 443
MspCI CTTAAG 1 cut(s) 389
MwoI GCNNNNNNNGC 5 cut(s) 55, 91, 100, 270, 314
NdeII GATC 2 cut(s) 19, 468
NlaIII CATG 1 cut(s) 377
NlaIV GGNNCC 2 cut(s) 280, 455
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 3 cut(s) 40, 237, 247
PkrI GCNGC 7 cut(s) 102, 105, 266, 319, 366, 426, 442
PleI GAGTC 2 cut(s) 172, 282
PpsI GAGTC 2 cut(s) 172, 282
Psp124BI GAGCTC 1 cut(s) 122
PspFI CCCAGC 2 cut(s) 341, 443
PspN4I GGNNCC 2 cut(s) 280, 455
PstI CTGCAG 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 336
SacI GAGCTC 1 cut(s) 122
SaqAI TTAA 2 cut(s) 390, 475
SatI GCNGC 7 cut(s) 101, 104, 265, 318, 365, 425, 441
Sau3AI GATC 2 cut(s) 19, 468
SchI GAGTC 2 cut(s) 173, 282
SduI GDGCHC 1 cut(s) 122
SetI ASST 9 cut(s) 108, 122, 177, 269, 300, 358, 366, 390, 445
SfcI CTRYAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 4 cut(s) 178, 268, 349, 389
SmoI CTYRAG 4 cut(s) 178, 268, 349, 389
Sse9I AATT 2 cut(s) 86, 472
SsiI CCGC 1 cut(s) 318
SstI GAGCTC 1 cut(s) 122
TaaI ACNGT 1 cut(s) 338
TaiI ACGT 1 cut(s) 300
TaqI TCGA 1 cut(s) 313
TasI AATT 2 cut(s) 86, 472
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 3 cut(s) 40, 237, 247
Tru1I TTAA 2 cut(s) 390, 475
Tru9I TTAA 2 cut(s) 390, 475
TseI GCWGC 6 cut(s) 100, 103, 264, 364, 424, 440
TspDTI ATGAA 3 cut(s) 53, 53, 244
Vha464I CTTAAG 1 cut(s) 389
XmnI GAANNNNTTC 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.